| [correlation of the excretion of mycoplasma and kinetics of antibodies detected by complement fixation, passive hemagglutination and rapid seroagglutination in mycoplasma mycoides subsp. mycoides sc experimental infection in cattle]. | during an experimental reproduction of cbpp, 5 inoculated cows and 5 contacts cows were bled twice a week and antibodies research was performed using complement fixation test (cft), passive haemagglutination test (phat) and slide agglutination test (sat). in the same period, trachobronchial washings were performed weekly to detect and to count mycoplasma mycoides subsp. mycoides sc. for four months these tests were used to compare antibodies kinetics with kinetics of mycoplasmas excretion. with ... | 1989 | 2485541 |
| sequence analysis of 16s rrna from mycoplasmas by direct solid-phase dna sequencing. | automated solid-phase dna sequencing was used for determination of partial 16s ribosomal dna sequences of mycoplasmas. the sequence information was used to establish phylogenetic relationships of 11 different mycoplasmas whose 16s rrna sequences had not been determined earlier. a biotinylated fragment corresponding to positions 344 to 939 in the escherichia coli sequence was generated by pcr. the pcr product was immobilized onto streptavidin-coated paramagnetic beads, and direct sequencing was p ... | 1994 | 7521158 |
| identification and characterization of is1296 in mycoplasma mycoides subsp. mycoides sc and presence in related mycoplasmas. | is1296, a new insertion sequence belonging to the is3 family of insertion elements has been identified in mycoplasma mycoides subsp. mycoides (mmm) biotype small colony (sc), the agent of contagious bovine pleuropneumonia (cbpp). is1296 is 1485-bp long and has 30-bp inverted repeats. it contains two open reading frames, orfa and orfb, which show significant similarities to the orfs which encode the transposase function of is elements of the is3 family, in particular is150 of escherichia coli. is ... | 1995 | 7628725 |
| [the epidemiology of contagious lung disease in switzerland]. | although contagious bovine pleuropneumonia (cbpp) has not been diagnosed in switzerland since 1895, anti-cbpp antibodies were detected in several cattle that had been imported from switzerland to italy. in order to clarify the current disease situation in switzerland, detailed epidemiological investigations were performed, including the control of 200,000 slaughter cattle in 108 abattoirs and serological testing of more than 2200 animals from 134 farms. the data collected in the slaughterhouses ... | 1995 | 7660094 |
| selective acylation of plasma membrane proteins of mycoplasma mycoides subsp. mycoides sc, the contagious bovine pleuropneumonia agent. | the plasma membrane of mycoplasma mycoides subsp. mycoides sc (strain kh3j) contains over 160 polypeptides with apparent molecular masses ranging from 14 to 125 kda and isoelectric point values (pis) from 5 to 9. in vivo labeling with [14c]-fatty acids revealed about 35 acylated polypeptides including the two major components p42 and p65 and displaying pis between 5.5 and 9.0, with a majority between 6.5 and 8. the amphiphilic nature of most of these acyl proteins was confirmed by triton x-114 p ... | 1996 | 8555942 |
| surveillance of contagious bovine pleuropneumonia in switzerland. | an active surveillance system for contagious bovine pleuropneumonia (cbpp) has been established in switzerland. the system is based on the detection of typical gross pathological lesions in the lungs of slaughtered cattle, followed by microbiological analysis of the sampled organs and sero-epidemiological investigation of the herd of origin for each suspect case. the programme was tested over six months. the prevalence of lung lesions detected in carcasses during this period in the 108 participa ... | 1995 | 8593396 |
| [complement fixation reaction for the serologic diagnosis of bovine contagious pleuropneumonia: application and interpretation of the results]. | contagious bovine pleuropneumonia (cbpp), caused by mycoplasma mycoides subsp. mycoides sc (small colony variant), is a disease which has been recognised for a long time. serological testing is used most often for diagnosing cbpp in live animals. this method is based on a complement fixation test (cft), the technique recommended by the office international des epizooties, which has an important role in detecting infected herds in regions known to be affected by the disease. although this serolog ... | 1995 | 8593397 |
| virulence of mycoplasma mycoides mycoides (sc) | | 1997 | 9106979 |
| development of a selective polymerase chain reaction assay for the detection of mycoplasma mycoides subsp. mycoides s.c. (contagious bovine pleuropneumonia agent). | a new selective assay for the detection of mycoplasma mycoides subsp. mycoides sc (mmmsc) via the polymerase chain reaction (pcr) has been developed. this test used two pcr assays: a control-pcr (myc-pcr) identifying the pathogen as a member of the mycoides cluster and the msc-pcr which is specific for mmmsc. the myc primers targeted a dna sequence of about 460 bp from all the 59 mycoides cluster-strains tested. no amplification occurred with bovine genomic dna or with the 11 other bacterial spe ... | 1994 | 9133058 |
| development of a sensitive nested pcr method for the specific detection of mycoplasma mycoides subsp. mycoides sc. | a specific and sensitive test for the detection of mycoplasma mycoides subsp. mycoides small colony type (sc), the aetiological agent of contagious bovine pleuropneumonia (cbpp) was developed using two nested pcr reactions. the pcr reactions are based on the nucleotide sequence of lipoprotein p72 of m. mycoides subsp. mycoides sc. the two specific oligonucleotide primer pairs were chosen to match those sequence segments of the p72 gene which differ most from the gene of the closely related lipop ... | 1997 | 9160324 |
| preliminary comparative studies on complement fixation, dot enzyme immunoassay, and western blotting for the detection of antibodies to mycoplasma mycoides subspecies mycoides sc in nigerian camel (camelus dromedarius). | the serological prevalence of antibodies to mycoplasma mycoides subsp. mycoides sc, the aetiological agent of contagious bovine pleuropneumonia (cbpp), was investigated in nigerian camels. fifty-eight serum samples were collected from slaughtered camels and examined by complement fixation (cft), dot enzyme immunoassay and western blots. fourteen of the slaughtered camels examined had pneumonic lesions. all sera examined were negative by cft but 7 (12.1%) and 4 (6.8%) were positive by dot enzyme ... | 1997 | 9270134 |
| a competitive elisa for the specific diagnosis of contagious bovine pleuropneumonia (cbpp). | a competitive elisa, using a specific monoclonal antibody, was designed to detect antibodies to mycoplasma mycoides subsp. mycoides sc, the agent of contagious bovine pleuropneumonia. one monoclonal antibody was found suitable for such a test, '117/5', it does not cross-react with any of the other mycoplasma species tested, furthermore, its binding is inhibited by positive sera. the cutoff, 50% of inhibition, was determined using a set of negative sera from cbpp-free areas. the sensitivity was c ... | 1998 | 9646449 |
| pcr and rflp methods for the specific detection and identification of mycoplasma mycoides subsp. mycoides sc. | | 1998 | 9711652 |
| [improvement and acceleration of the diagnosis of contagious bovine pleuropneumonia by direct detection of the microbe using polymerase chain reaction (pcr)]. | a diagnostic procedure is described for the detection of mycoplasma mycoides subsp. mycoides sc, the causative agent of contagious bovine pleuropneumonia. dna extracted from clinical samples was investigated by the polymerase chain reaction (pcr) using at least 2 different primer pairs, one species-specific and another one specific for the class of mollicutes. using this method, the time required for detection of the pathogen was reduced to 2 days, whereas with traditional diagnostic methods (cu ... | 1998 | 9741182 |
| genetic and serological analysis of the immunogenic 67-kda lipoprotein of mycoplasma sp. bovine group 7. | the gene encoding a lipoprotein of 67 kda, named p67, was cloned from mycoplasma sp. bovine group 7 strain pg50 and expressed in escherichia coli k12. analysis of the amino acid sequence derived from the dna sequence of the p67 gene revealed a typical prokaryotic signal peptidase ii membrane lipoprotein lipid attachment site and a transmembrane structure domain in the leader sequence at the amino-terminal end of the protein. protein p67 showed 91% identical amino acid residues to the lipoprotein ... | 1998 | 9766210 |
| antigen heterogeneity among mycoplasma mycoides subsp. mycoides sc isolates: discrimination of major surface proteins. | the protein and antigen profiles of 60 isolates, strains and the type strain pg1 of mycoplasma mycoides subsp. mycoides s.c. were compared by sodium dodecyl sulphate polyacrylamide gel electrophoresis and immunoblot analysis. analysis using contagious bovine pleuropneumonia antisera and hyperimmune rabbit sera against several representative strains revealed some differences in protein profiles and variability in antigens among strains from different geographic regions. the most common antigenic ... | 1998 | 9810618 |
| detection of mycoplasma mycoides subspecies mycoides sc in clinical material by a rapid colorimetric pcr. | a colorimetric polymerase chain reaction (pcr) test for the detection of mycoplasma mycoides subsp. mycoides sc (mmm sc), the cause of contagious bovine pleuropneumonia (cbpp), was used to test clinical samples from suspect and known cases of the disease. a commercially available detection kit, pleuro trap from amrad, australia, which captures amplified double stranded dna in the wells of a microtitre plate was used. tissues tested from animals ranging from a variety of anatomical locations incl ... | 1999 | 10024429 |
| mycoplasma mycoides subsp. mycoides sc identification by pcr in sperm of seminal vesiculitis-affected bulls. | in this study, by using the polymerase chain reaction (pcr) diagnosis for the detection and identification of mycoplasma, we investigated mycoplasmas contaminating the semen of yearling bulls affected by seminal vesiculitis. the bulls presented neither subclinical nor clinical contagious bovine pleuropneumonia signs and the complement fixation test for specific antibodies was negative. furthermore, we have investigated mycoplasmas isolated from semen of healthy breeding bulls of several breeds a ... | 1999 | 10543380 |
| diagnosis of contagious bovine pleuropneumonia by pcr-laser- induced fluorescence and pcr-restriction endonuclease analysis based on the 16s rrna genes of mycoplasma mycoides subsp. mycoides sc. | as contagious bovine pleuropneumonia (cbpp) is spreading fast in many african countries, there is an increasing demand for rapid and sensitive diagnostic methods that can be used to confirm the initial diagnosis based on clinical symptoms or pathological findings. two pcr-based diagnostic systems for identification of the infectious agent, mycoplasma mycoides subsp. mycoides sc (m. mycoides sc), in various samples are presented. both systems involve group-specific amplification of the two 16s rr ... | 1999 | 10565890 |
| genomic and antigenic differences between the european and african/australian clusters of mycoplasma mycoides subsp. mycoides sc. | mycoplasma mycoides subsp. mycoides small-colony type (sc), the aetiological agent of contagious bovine pleuropneumonia (cbpp), can be grouped into two major, epidemiologically distinct, clusters. one cluster contains strains isolated from different european countries since 1980 and a second cluster contains african and australian strains collected over the last 50 years. genetic analysis of representative strains from the two clusters revealed a genomic segment of 8.84 kb, located close to a co ... | 2000 | 10708386 |
| phylogeny of the mycoplasma mycoides cluster as shown by sequencing of a putative membrane protein gene. | the mycoplasma mycoides cluster is made of six species that are closely related both genetically and phenotypically. two are of particular importance, m. mycoides subsp. mycoides sc causing contagious bovine pleuropneumonia and m. capricolum subsp. capripneumoniae causing contagious caprine pleuropneumonia. the sequences of a putative membrane protein gene and partial flanking open reading frames have been obtained from various strains in this cluster, including all reference strains. sequence a ... | 2000 | 10727835 |
| antigenic and genetic characterization of lipoprotein lppq from mycoplasma mycoides subsp. mycoides sc. | lipoprotein lppq, a predominant 48-kda antigen, and its corresponding gene, lppq, were characterized in mycoplasma mycoides subsp. mycoides sc, the etiological agent of contagious bovine pleuropneumonia. the lppq gene is specific to m. mycoides subsp. mycoides sc and was found in the type strain and in field strains isolated in europe, africa, and australia, as well as in vaccinal strains. lppq is encoded as a precursor with a consensus sequence for prokaryotic signal peptidase ii and a lipid at ... | 2000 | 10882657 |
| specific pcr identification of the t1 vaccine strains for contagious bovine pleuropneumonia. | a specific pcr test for the identification of the vaccine strains t1, t1/44 and t1sr, was developed. this pcr reaction is based on variations of dna sequences in a region flanking one is1296 copy. the specific primer pair mmmscp1-t1m2 amplifies a 700-bp long dna fragment in the t1 vaccine strains and gives no amplification with the 60 other mycoplasma mycoides subsp. mycoides sc strains tested. this pcr will permit to distinguish the t1 strain from all other vaccine strains and therefore avoid p ... | 2000 | 10970724 |
| genetic and biochemical characterization of glycerol uptake in mycoplasma mycoides subsp. mycoides sc: its impact on h(2)o(2) production and virulence. | highly virulent strains of mycoplasma mycoides subsp. mycoides sc belonging to the african cluster contain an operon with the genes gtsa, gtsb, and gtsc, encoding membrane atp binding cassette transporter proteins gtsa, gtsb, and gtsc, which are involved in glycerol transport. strain afadé from the african cluster incorporated [u-(14)c]glycerol with a time-dependent increase. the less virulent strain l2 of the european cluster, which lacks gtsb and gtsc, failed to incorporate glycerol. antibodie ... | 2001 | 11139200 |
| effect of hepes buffer systems upon the ph, growth and survival of mycoplasma mycoides subsp. mycoides small colony (mmmsc) vaccine cultures. | the use of a buffer system based on n-[2-hydroxyethyl]piperazine-n'-[2-ethanesulfonic acid] (hepes), in conjunction with standard gourlay's culture medium was investigated for the growth and maintenance of mycoplasma mycoides subsp. mycoides sc vaccine strain t(1)44. when the initial ph of the culture medium was adjusted to 8.0, 0.075 m hepes-naoh was found to be sufficient to prevent the ph falling below 7.1 at any stage during the growth cycle, even in the presence of 0.5% glucose. compared to ... | 2001 | 11470376 |
| serodiagnosis and monitoring of contagious bovine pleuropneumonia (cbpp) with an indirect elisa based on the specific lipoprotein lppq of mycoplasma mycoides subsp. mycoides sc. | an indirect elisa, based on the specific and strongly antigenic recombinant peptide of the n'-terminal half of the lipoprotein lppq from mycoplasma mycoides subsp. mycoides small colony type (sc) was developed for the detection of antibodies to m. mycoides subsp. mycoides sc. it was evaluated for its suitability for serodiagnosis and monitoring of contagious bovine pleuropneumonia (cbpp). the recombinant peptide containing poly-histidine residue tails was expressed in escherichia coli and subseq ... | 2002 | 11731172 |
| novel deoxynucleoside-phosphorylating enzymes in mycoplasmas: evidence for efficient utilization of deoxynucleosides. | mycoplasmas are unable to synthesize purine and pyrimidine bases de novo. therefore, salvage of existing nucleosides and bases is essential for their survival. four mycoplasma species were studied with regard to their ability to phosphorylate deoxynucleosides. high levels of thymidine kinase (tk), deoxycytidine kinase (dck), deoxyguanosine kinase (dgk) and deoxyadenosine kinase (dak) activities were detected in extracts from mycoplasma pneumoniae, mycoplasma mycoides subsp. mycoides sc (m. mymys ... | 2001 | 11737647 |
| re-suspension of t(1)44 vaccine cultures of mycoplasma mycoides subsp mycoides sc in 1 molar mgso(4) causes a drop in ph and a rapid reduction in titre. | the effect of reconstituting freeze-dried vaccine cultures of mycoplasma mycoides subsp. mycoides small colony biotype (mmmsc) strain t(1)44 grown in standard vaccine medium using variable quantities of un-buffered solutions of 1 m mgso(4) (the current o.i.e.-recommended procedure) has been investigated. compared to the culture ph prior to desiccation, a drop of up to 2.2 ph units was observed, dependent upon the volume and ph of 1 m mgso(4) used (1-30 x original culture volume, using 1 m mgso(4 ... | 2002 | 12381459 |
| genetic and antigenic characterisation of elongation factor tu from mycoplasma mycoides subsp. mycoides sc. | specific serodiagnosis of contagious bovine pleuropneumonia (cbpp) is hampered by the low antibody titers against mycoplasma mycoides subsp. mycoides small-colony type (mmmsc) antigens in calf serum due to persistent infections and by the existence of cross-reactions among the members of the mycoides cluster. in order to identify potential diagnostic antigens, we have constructed a genomic library from mmmsc which was screened with antibodies from naturally-infected animals. using this strategy, ... | 2002 | 12383637 |
| rapid detection of contagious bovine pleuropneumonia by a mycoplasma mycoides subsp. mycoides sc capsular polysaccharide-specific antigen detection latex agglutination test. | a latex agglutination test (lat) has been developed for the diagnosis of contagious bovine pleuropneumonia (cbpp). the latex microspheres were coated with mmmsc polyclonal immunoglobulin g antiserum and detected mmmsc antigen in the serum of cattle infected with cbpp and in growth medium containing mmmsc. the specific antigen recognized by this test appeared to be the capsular polysaccharide (cps). the lat recognized all 23 strains of mmmsc examined in this study, with a sensitivity level of 2 n ... | 2003 | 12626448 |
| molecular epidemiology of contagious bovine pleuropneumonia by multilocus sequence analysis of mycoplasma mycoides subspecies mycoides biotype sc strains. | contagious bovine pleuropneumonia is a bacterial disease caused by mycoplasma mycoides subsp. mycoides sc (mmmsc), and included in list a of the office international des epizooties. it is one of the major constraints to cattle raising in sub-saharan and south-western africa and also a threat to all countries currently free of the disease. mmmsc strains were considered very homogeneous until 1995, when various techniques such as enzymatic restriction of whole dna or southern blotting showed that ... | 2003 | 12713894 |
| antigenic and genetic characterisation of lipoprotein lppc from mycoplasma mycoides subsp. mycoides sc. | lipoprotein lppc, an immunodominant antigen, and its corresponding gene lppc were characterised in mycoplasma mycoides subspecies mycoides small colony (sc) type, the etiological agent of contagious bovine pleuropneumonia (cbpp). the lppc gene was found in the type strain of m. mycoides subsp. mycoides sc and in field strains isolated in europe, africa, and australia, as well as in vaccine strains. southern blot analysis indicated the presence of at least four copies of lppc in the genome of m. ... | 2003 | 14746771 |
| the genome sequence of mycoplasma mycoides subsp. mycoides sc type strain pg1t, the causative agent of contagious bovine pleuropneumonia (cbpp). | mycoplasma mycoides subsp. mycoidessc (mmymysc)is the etiological agent of contagious bovine pleuropneumonia (cbpp), a highly contagious respiratory disease in cattle. the genome of mmymy sc type strain pg1(t) has been sequenced to map all the genes and to facilitate further studies regarding the cell function of the organism and cbpp. the genome is characterized by a single circular chromosome of 1211703 bp with the lowest g+c content (24 mole%)and the highest density of insertion sequences (13 ... | 2004 | 14762060 |
| contagious bovine pleuropneumonia (cbpp) caused by vaccine strain t1/44 of mycoplasma mycoides subsp. mycoides sc. | a study was carried out on four adult cattle to assess the pathogenicity of mycoplasma mycoides subsp. mycoides sc strain t1/44, currently used as a vaccine for the control of contagious bovine pleuropneumonia (cbpp) in namibia. post mortem examination 9 weeks after endobronchial inoculation of the vaccine strain to three of the four animals revealed unilateral pleuropneumonic lesions, pleuritis and well-developed sequesters in two of the three inoculated animals and several small sequesters sur ... | 2004 | 15036531 |
| differential clustering of mycoplasma mycoides subsp. mycoides sc strains by pcr-rea of the bgl locus. | in order to develop a specific tool differentiating the african field strains of mycoplasma mycoides subsp. mycoides sc from other potentially less virulent strains, including the vaccine strains, we have developed a pcr followed by a restriction enzyme analysis (pcr-rea). this approach also differentiates the african field strains from the australian strains and the type strain pg1. the genomic marker detected by the pcr-rea is based on a single nucleotide change in the bgl gene that codes for ... | 2004 | 15145506 |
| mycoplasma bovis shares insertion sequences with mycoplasma agalactiae and mycoplasma mycoides subsp. mycoides sc: evolutionary and developmental aspects. | three new insertion elements, ismbov1, ismbov2 and ismbov3, which are closely related to ismag1 (mycoplasma agalactiae), ismmy1 and is1634 (both mycoplasma mycoides subsp. mycoides sc), respectively, have been discovered in mycoplasma bovis, an important pathogen of cattle. southern blotting showed that the genome of m. bovis harbours 6-12 copies of ismbov1, 11-15 copies of ismbov2 and 4-10 copies of ismbov3, depending on the strain. a fourth insertion element, the is30-like element, is present ... | 2005 | 15837379 |
| gamma interferon-producing cd4 t-cells correlate with resistance to mycoplasma mycoides subsp. mycoides s.c. infection in cattle. | contagious bovine pleuropneumonia (cbpp), caused by mycoplasma mycoides subsp. mycoides sc (mmmsc), is one of the most significant cattle disease in africa. the control measures, which led to eradication from numerous countries are not feasible in africa where the only prophylaxis relies on vaccination. however, the attenuated vaccines, used up to now in africa, are of low efficiency. the development of an improved vaccine is, therefore, a necessity. the purpose of this study was to compare some ... | 2005 | 15946743 |
| [expression, purification and antigen activity analysis of the n-terminal domain of lipoprotein lppq of mycoplasma mycoide subsp. mycoides sc]. | the gene sequence coding the n-terminal domain of lppq was amplified from mycoplasma mycoides subsp. mycoides sc (mmmsc) hvri x strain by pcr using special primers and was cloned into the ecor i /sal i sites of pet32a vector to construct the expression recombinant plasmids. the recombinant plasmids were indentified by restriction digestion, pcr and sequence analysis. the gene was overexpressed in escherichia coli bl21 (de3) host cell and the soluble protein was purified with ni-nta his. bind pur ... | 2005 | 16342778 |
| biofilm formation by mycoplasma species and its role in environmental persistence and survival. | although mycoplasmas possess a very limited genome, little is known about their virulence mechanisms and methods of persistence in the host. examination of a wide range of mycoplasma species found considerable variation in their ability to form a biofilm. mycoplasma putrefaciens, m. cottewii, m. yeatsii, m. agalactiae and m. bovis produced prolific biofilms. conversely, the highly pathogenic mycoplasma and causative agent of contagious bovine pleuropneumonia, mycoplasma mycoides subsp. mycoides ... | 2006 | 16549656 |
| danofloxacin (advocin) reduces the spread of contagious bovine pleuropneumonia to healthy in-contact cattle. | contagious bovine pleuropneumonia (cbpp), caused by mycoplasma mycoides subsp. mycoides sc (mmmsc), is one of the most important diseases of cattle in sub-saharan africa. the live t1/44 vaccine is normally used for its control but produces only transient protection and gives rise to adverse reactions. the present study evaluated the efficacy of danofloxacin (2.5% advocintrade mark, pfizer ltd.) for the treatment of naturally infected cattle and in the prevention of cbpp transmission to in-contac ... | 2006 | 16624356 |
| pulmonary and serum antibody responses elicited in zebu cattle experimentally infected with mycoplasma mycoides subsp. mycoides sc by contact exposure. | the purpose of the present study was to characterize the mycoplasma mycoides subsp. mycoides small colony (mmmsc)-specific humoral immune response at both systemic and local levels in cattle experimentally infected with mmmsc, for a better understanding of the protective immune mechanisms against the disease. the disease was experimentally reproduced in zebu cattle by contact. clinical signs, postmortem and microbiological findings were used to evaluate the degree of infection. serum and bronchi ... | 2006 | 16820137 |
| molecular mechanisms of pathogenicity of mycoplasma mycoides subsp. mycoides sc. | mycoplasma mycoides subsp. mycoides sc, the aetiological agent of contagious bovine pleuropneumonia (cbpp), is considered the most pathogenic of the mycoplasma species. its virulence is probably the result of a coordinated action of various components of an antigenically and functionally dynamic surface architecture. the different virulence attributes allow the pathogen to evade the host's immune defence, adhere tightly to the host cell surface, persist and disseminate in the host causing mycopl ... | 2007 | 17157043 |
| beta-d-glucoside utilization by mycoplasma mycoides subsp. mycoides sc: possible involvement in the control of cytotoxicity towards bovine lung cells. | contagious bovine pleuropneumonia (cbpp) caused by mycoplasma mycoides subsp. mycoides small-colony type (sc) is among the most serious threats for livestock producers in africa. glycerol metabolism-associated h2o2 production seems to play a crucial role in virulence of this mycoplasma. a wide number of attenuated strains of m. mycoides subsp. mycoides sc are currently used in africa as live vaccines. glycerol metabolism is not affected in these vaccine strains and therefore it does not seem to ... | 2007 | 17439646 |
| domain analysis of lipoprotein lppq in mycoplasma mycoides subsp. mycoides sc. | the lipoprotein lppq is the most prominent antigen of mycoplasma mycoides subsp. mycoides small colony type (sc) during infection of cattle. this pathogen causes contagious bovine pleuropneumonia (cbpp), a devastating disease of considerable socio-economic importance in many countries worldwide. the dominant antigenicity and high specificity for m. mycoides subsp. mycoides sc of lipoprotein lppq have been exploited for serological diagnosis and for epidemiological investigations of cbpp. scannin ... | 2008 | 17674137 |
| biofilm-grown mycoplasma mycoides subsp. mycoides sc exhibit both phenotypic and genotypic variation compared with planktonic cells. | biofilm formation where bacterial cells adhere to a surface and surround themselves in a polysaccharide matrix is thought to be an important factor in disease initiation and persistence for many bacterial species. we have examined biofilm formation by mycoplasma mycoides subsp. mycoides small colony using a simple model without an air/liquid interface and have found that adherent mmm sc was more resistant to many stresses, including heat, osmotic shock and oxidative stress. biofilms of mmm sc al ... | 2008 | 18191921 |
| expression and immunogenicity of six putative variable surface proteins in mycoplasma mycoides subsp. mycoides sc. | variable surface protein vmm and five vmm-type proteins from mycoplasma mycoides subsp. mycoides sc were analysed to determine whether these proteins are expressed in vivo in animals affected by contagious bovine pleuropneumonia (cbpp) and in vitro. recombinant versions of these proteins were constructed and expressed in escherichia coli after mutation of the tga trp codons to tgg. these proteins were then analysed by dot and western blotting with sera from cbpp-affected cattle. furthermore, aff ... | 2008 | 18227258 |
| genotyping of mycoplasma mycoides subsp. mycoides sc by multilocus sequence analysis allows molecular epidemiology of contagious bovine pleuropneumonia. | mycoplasma mycoides subsp. mycoides sc (mmmsc) is the etiological agent of contagious bovine pleuropneumonia (cbpp). although eradicated in most developed countries, the disease reappeared in europe in the 1990s. this reappearance may have been caused either by importation from sub-saharan africa, where cbpp is still endemic, or by the reemergence of virulent strains in europe, as suggested by earlier studies. a multilocus sequence analysis scheme has been developed to address this issue and, mo ... | 2008 | 18258170 |
| unmarked insertional mutagenesis in the bovine pathogen mycoplasma mycoides subsp. mycoides sc: characterization of a lppq mutant. | mycoplasma mycoides subspecies mycoides small colony (sc) is the aetiologic agent of contagious bovine pleuropneumonia (cbpp), a respiratory disease causing important losses in cattle production. the publication of the genome sequence of m. mycoides subsp. mycoides sc should facilitate the identification of putative virulence factors. however, real progress in the study of molecular mechanisms of pathogenicity also requires efficient molecular tools for gene inactivation. in the present study, w ... | 2008 | 18667575 |
| specific real-time pcr assays for the detection and quantification of mycoplasma mycoides subsp. mycoides sc and mycoplasma capricolum subsp. capripneumoniae. | contagious bovine pleuropneumonia and contagious caprine pleuropneumonia are two severe respiratory infections of ruminants due to infection by mycoplasma mycoides subsp. mycoides sc (mmmsc) and mycoplasma capricolum subsp. capripneumoniae (mccp), respectively. they are included in the oie list of notifiable diseases. here we describe the development of rapid, sensitive, and specific real-time pcr assays for the detection and quantification of mmmsc and mccp dna. mmmsc pcr primers were designed ... | 2008 | 18678244 |
| functional and antigenic properties of glpo from mycoplasma mycoides subsp. mycoides sc: characterization of a flavin adenine dinucleotide-binding site deletion mutant. | l-alpha-glycerophosphate oxidase (glpo) plays a central role in virulence of mycoplasma mycoides subsp. mycoides sc, a severe bacterial pathogen causing contagious bovine pleuropneumonia (cbpp). it is involved in production and translocation of toxic h(2)o(2) into the host cell, causing inflammation and cell death. the binding site on glpo for the cofactor flavin adenine dinucleotide (fad) has been identified as gly(12)-gly(13)-gly(14)-ile(15)-ile(16)-gly(17). recombinant glpo lacking these six ... | 2009 | 19364458 |
| identification of genes coding for b cell antigens of mycoplasma mycoides subsp. mycoides small colony (mmmsc) by using phage display. | contagious bovine pleuropneumonia (cbpp) is a mycoplasmal disease caused by mycoplasma mycoides subsp. mycoides sc (mmmsc). since the disease is a serious problem that can affect cattle production in parts of africa, there is a need for an effective and economical vaccine. identifying which of the causative agent's proteins trigger potentially protective immune responses is an important step towards developing a subunit vaccine. accordingly, the purpose of this study was to determine whether pha ... | 2009 | 19818124 |
| detection of mycoplasma mycoides subsp. mycoides sc in bronchoalveolar lavage fluids of cows based on a taqman real-time pcr discriminating wild type strains from an lppq(-) mutant vaccine strain used for diva-strategies. | contagious bovine pleuropneumonia (cbpp) is the most serious cattle disease in africa, caused by mycoplasma mycoides subsp. mycoides small-colony type (sc). cbpp control strategies currently rely on vaccination with a vaccine based on live attenuated strains of the organism. recently, an lppq(-) mutant of the existing vaccine strain t1/44 has been developed (janis et al., 2008). this t1lppq(-) mutant strain is devoid of lipoprotein lppq, a potential virulence attribute of m. mycoides subsp. myco ... | 2010 | 20381545 |
| thymidylate synthases of mycoplasma mycoides subsp. mycoides sc and ureaplasma parvum are flavin-dependent. | mycoplasma mycoides subsp. mycoides small colony type (m. mycoides subsp. mycoides sc) is the causative agent of contagious bovine pleuropneumonia (cbpp), one of the most serious bacterial diseases in cattle and buffalo. ureaplasma parvum (u. parvum) colonizes the human urogenital tract, and has been associated with urethritis and premature birth. the de novo synthesis of thymidylate (dtmp) is essential and catalyzed by thymidylate synthase (ts), encoded by either the thya or the thyx genes. no ... | 2010 | 20413228 |
| molecular diversity assessed by vntr and is1296 typing of historical mycoplasma mycoides subsp. mycoides sc strains. | the last case of contagious bovine pleuropneumonia (cbpp), caused by mycoplasma mycoides subsp. mycoides sc (mmmsc), in europe was reported in portugal in 1999. however, in view of its insidious nature, it is still possible that cbpp could re-emerge. despite differences in animal host and geographical origin, most of the european mmmsc field isolates were traditionally considered to be very homogeneous. in the present study we performed a retrospective variable-number tandem repeat (vntr) and is ... | 2010 | 20542647 |
| development of a bead-based multiplex pcr assay for the simultaneous detection of multiple mycoplasma species. | we describe the development and analytical validation of a 7-plex polymerase chain reaction assay coupled to a bead-based liquid suspension array for detection of multiple ruminant mycoplasma spp. the assay employs a combination of newly designed and previously validated primer-probe sets that target genetic loci specific for mycoplasma bovis, mycoplasma mycoides cluster, mycoplasma mycoides subsp. mycoides sc (mmmsc) and mycoplasma capricolum subspecies capripneumoniae (mccp). analytical sensit ... | 2011 | 21726966 |
| assessment of a novel multiplex real-time pcr assay for the detection of the cbpp agent mycoplasma mycoides subsp. mycoides sc through experimental infection in cattle. | abstract: | 2011 | 21838878 |