studies on mycoplasma mycoides subsp. mycoides (lc) in lambs and calves.six cesarean-derived lambs were inoculated either with 4.5 x 10(4), 4.5 x 10(6) or 4.5 x 10(8) mycoplasma mycoides subsp. mycoides intratracheally. one animal receiving the intermediate dose died four days post-inoculation, the two receiving the high dose died six days postinoculation, while one receiving the low dose died eight days postinoculation. the two surviving lambs were challenged on day 20 postinoculation with 1 x 10(8) organisms subcutaneously and 2 x 10(9) organisms intravenously. on ...19853893663
mycoplasma mycoides subsp mycoides, lc type, from goats in italy.a mycoplasma identified as mycoplasma mycoides subsp mycoides lc type was isolated from an outbreak of caprine pleuropneumonia and serofibrinous peritonitis. this isolate was the first to be reported in italy. experimentally, a rapidly fatal condition followed its inoculation into goats and sheep but inoculation of calves did not lead to clinically apparent infection. although the organism was recovered for up to 45 days following experimental inoculation of calves, pathological changes were lim ...19826760527
the identification of mycoplasma mycoides mycoides lc as the aetiological agent of balanoposthitis and vulvovaginitis in sheep in south africa.clinical ulcerative balanoposthitis and vulvovaginitis was experimentally reproduced in 14 sheep infected with a mycoplasma mycoides mycoides lc field strain, isolated from the straussheim dorper stud. the study encompassed a series of field observations, a therapeutic trial and experimental investigations. a wide range of bacteria and various mycoplasma spp., but no viruses, were isolated from a large number of infected animals.19938392679
genetic and serological analysis of lipoprotein lppa in mycoplasma mycoides subsp. mycoides lc and mycoplasma mycoides subsp. capri.the genes encoding the 62-kda lipoproteins from the mycoplasma mycoides subsp. mycoides large-colony type (lc) strain y-goat and the m. mycoides subsp. capri strain pg3 were cloned and analyzed by sequencing. these two lipoproteins have been named lppa[mmymylc] and lppa[mmyca], and their corresponding genes have been named lppa[mmymylc] and lppa[mmyca], respectively. the nucleotide and deduced amino acid sequences of these two lipoproteins showed a very high degree of similarity between these tw ...199910066658
host specificity of mollicutes oric plasmids: functional analysis of replication origin.recently, artificial oric plasmids containing the chromosomal dnaa gene and surrounding dnaa box sequences were obtained for the mollicutes spiroplasma citri and mycoplasma pulmonis. in order to study the specificity of these plasmids among mollicutes, a set of similar oric plasmids was developed for three mycoplasmas belonging to the mycoides cluster, mycoplasma mycoides subsp. mycoides lc (mmmlc), m.mycoides subsp. mycoides sc (mmmsc) and mycoplasma capricolum subsp. capricolum. mycoplasmas fr ...200314602921
characterisation of protein and antigen variability among mycoplasma mycoides subsp. mycoides (lc) and mycoplasma agalactiae field strains by sds-page and immunoblotting.mycoplasma mycoides subsp. mycoides (lc) (mmm lc) and mycoplasma agalactiae are the most important mycoplasma species involved in the contagious agalactia syndrome. a total of 25 field strains from spain and the two type strains were analysed by sds-page and immunoblotting. two polyclonal antisera (pabs) raised against a pool of strains of each mycoplasma species were used. the results revealed a high degree of protein variability among the field strains. the type strain of mmm lc appeared to be ...200616624721
mycoplasma mycoides subsp. capri and mycoplasma mycoides subsp. mycoides lc can be grouped into a single subspecies.mycoplasma mycoides subsp. capri and mycoplasma mycoides subsp. mycoides lc can be combined into one taxon on the basis of several contributions on both dna sequence and protein analyses reported in the literature. moreover, for the differentiation and identification of mycoplasmas of the "mycoides cluster", we investigated the rpob gene, encoding the beta-subunit of the rna polymerase. a segment of 527 bp of the rpob gene was amplified from 31 strains of ruminant mycoplasmas by pcr. the nucleot ...200616973118
field trial of two dual vaccines against mycoplasma agalactiae and mycoplasma mycoides subsp. mycoides (large colony type) in goats.two vaccines against mycoplasma agalactiae and mycoplasma mycoides subsp. mycoides (lc type) were developed using inactivated strains selected in previous characterization studies. the vaccines differed in terms of the adjuvants used: aluminium hydroxide (vaccine a) or aluminium hydroxide plus purified saponin (vaccine b). these vaccines were tested on 60 pregnant goats and 60 seronegative kids that were challenged by placing in a herd with a history of caprine contagious agalactia (cca). our fi ...200717239497
in vitro antimycoplasmal activity of six jordanian medicinal plants against three mycoplasma species.the in vitro effect of six jordanian traditional medicine plant methanolic extracts were tested against 32 isolates of mycoplasma species; mycoplasma mycoides subsp. mycoides lc (6), mycoplasma capricolum subsp. capricolum (8) and m. putrefaciens (18), all isolated from either nasal swabs or milk, from sheep and goats in different regions in jordan. all mycoplasma species showed susceptibility to artemisia herba-alba and artemisia arborescens with mic ranges from 3.125-12.5 mg/ml. allium sativum ...200717969714
mycoplasma leachii sp. nov. as a new species designation for mycoplasma sp. bovine group 7 of leach, and reclassification of mycoplasma mycoides subsp. mycoides lc as a serovar of mycoplasma mycoides subsp. capri.the mycoplasma mycoides cluster consists of six pathogenic mycoplasmas causing disease in ruminants, which share many genotypic and phenotypic traits. the m. mycoides cluster comprises five recognized taxa: mycoplasma mycoides subsp. mycoides small colony (mmmsc), m. mycoides subsp. mycoides large colony (mmmlc), m. mycoides subsp. capri (mmc), mycoplasma capricolum subsp. capricolum (mcc) and m. capricolum subsp. capripneumoniae (mccp). the group of strains known as mycoplasma sp. bovine group ...200919502315
isolation and characterisation of an indian strain of mycoplasma mycoides subsp. mycoides type lc from a case of caprine arthritis.isolation and characterisation of an indian strain of mycoplasma mycoides subsp. mycoides lc from a case of caprine arthritis is reported in the study. the isolate was identified based on biochemical, digitonin sensitivity and growth inhibition tests. the virulence of the organism was studied by pathogenicity test in mice and goat. the antigenic and genomic profile of the isolate was compared with that of the standard strain (y-goat). using different sets of primers, polymerase chain reaction wa ...200415178001
inactivation of mycoplasma species involved in contagious agalactia.the suitability of 5 agents for the inactivation of different field strains of the four mycoplasma species associated with contagious agalactia syndrome in goats, i.e. mycoplasma agalactiae, mycoplasma mycoides subsp. mycoides lc, mycoplasma capricolum subsp. capricolum and mycoplasma putrefaciens, was investigated. immunoprophylaxis of this syndrome is still based on inactivated vaccines, which traditionally use formalin as the inactivating agent. moreover, the limited information existing abou ...200414964115
disease caused by mycoplasma mycoides subspecies mycoides lc in hungarian goat herds.the occurrence of a goat disease caused by mycoplasma mycoides subsp. mycoides lc in hungary is reported. the disease occurred in two goat herds in the spring of 1999. in one herd 25% of the 4-12 weeks old kids (10 animals) while in the other herd 33% of the 6-12 weeks old kids (20 animals) became affected. the goat kids developed polyarthritis. the most severe lesions developed in the carpal joints. all animals died after 3-8 days of disease. four dead kids were necropsied. all of them had sero ...200011402711
caprine mycoplasmal mastitis in a study to investigate the current status of intramammary mycoplasmosis in caprine udders in nigeria, a total of 57 and 24 milk samples were collected from udders of goats affected by mastitis and from apparently normal goats' udders, respectively. acute and chronic mastitis were more commonly observed in goats between 1 and 3 years old. mycoplasma agalactiae and mycoplasma capricolum occurred at a significantly higher rate (p<0.05) in udders affected by mastitis than in normal healthy udders ...200111163719
relationship between mycoplasma mycoides subsp. mycoides ('large-colony' strains) and m. mycoides subsp. capri, as indicated by numerical analysis of one-dimensional sds-page protein patterns.twenty-five strains classified as mycoplasma mycoides subsp. mycoides lc or subsp. capri have been compared by one-dimensional sds-page of their cellular proteins. a computerized numerical analysis revealed that the protein patterns of all but two aberrant strains formed one large phenon that separated clearly from representatives of the four other members of the 'm. mycoides cluster' at a similarity level (s) of 66% and which remained undivided at up to 78% s. at higher similarity levels, these ...19892693591
demonstration of cross-reactive antigens in f38 and related mycoplasmas by enzyme-linked immunosorbent assay (elisa) and immunoblotting.the elisa and an immunoblotting technique were used to study f38-type mycoplasmas - an important cause of contagious caprine pleuropneumonia - and a number of related mycoplasma species, subspecies, types or serogroups. two-way elisa cross-reactivity was demonstrated between five mycoplasmas, namely strain f38, mycoplasma mycoides subsp. mycoides (lc strain), m. equigenitalium, m. primatum and bovine serogroup 7. in addition one-way cross-reactivity was demonstrated between f38 and each of the f ...19852410491
experimental infection of goats with an unusual strain of mycoplasma mycoides subsp. capri isolated in jordan.goats were infected experimentally with a mycoplasma (the "irbid" strain) isolated previously from a goat with contagious agalactia in northern jordan. the strain was unusual in that, although it had been identified by molecular methods as mycoplasma mycoides subsp. mycoides lc/mycoplasma mycoides subsp. capri, it showed no inhibition of growth by any of the hyperimmune rabbit antisera conventionally used to speciate members of the mycoplasma mycoides cluster. animals were infected either intrat ...200919446837
effects on goat milk quality of the presence of mycoplasma spp. in herds without symptoms of contagious agalactia.this study was designed to assess the possible effects of mycoplasmas on the quality of milk produced by goat herds in a contagious agalactia (ca) endemic area with absence of classical symptoms. several factors related to milk quality (percentages of fat, total protein, lactose and total solids, standard plate counts (spc) and presence of staphylococcus aureus) were compared in mycoplasma-infected and non-infected herds. to define the ca status of 26 herds on the island of lanzarote (spain), wh ...200918922196
a pcr for the detection of mycoplasmas belonging to the mycoplasma mycoides cluster: application to the diagnosis of contagious agalactia.contagious agalactia is a mycoplasmal infection caused by mycoplasma agalactiae, mycoplasma mycoides subsp. mycoides lc, m. mycoides subsp. capri, mycoplasma capricolum subsp. capricolum and mycoplasma putrefaciens. identification of the causative organisms is usually performed by isolation and classical biochemical and serological tests, though this is a lengthy and cumbersome process for mycoplasmas. specific pcr assays have been developed for the identification of mycoplasma agalactiae and m. ...200717606362
the in vitro effect of six antimicrobials against mycoplasma putrefaciens, mycoplasma mycoides subsp. mycoides lc and mycoplasma capricolum subsp. capricolum isolated from sheep and goats in jordan.respiratory disease in sheep and goats is a major problem in jordan and is often associated with mycoplasma species. without effective vaccines, control is mainly by chemotherapy, but the uncontrolled use of antimicrobials has led to concerns about the potential development of antimicrobial resistance. the in vitro effect of chloramphenicol, florfenicol, enrofloxacin, tylosin, erythromycin and oxytetracycline was determined against 32 isolates of mycoplasma species-m. mycoides subsp. mycoides lc ...200617405622
isolation of mycoplasma mycoides subsp. mycoides (lc variant, y-goat) from naturally aborted bovine strains of mycoplasma mycoides subsp. mycoides (lc, y-goat) were isolated from 4 of 85 (4.7%) cases of bovine abortion. the biochemical characters of all the isolates were homogenous (except for one sucrose negative isolate), but variation in sensitivity to neomycin, kanamycin and streptomycin were noted. the only gross lesions of internal organs in aborted fetuses were congestion of liver and lungs, and hemorrhagic patches on the heart surface. significant microscopic lesions were encount ...198916726715
cloning and characterization of the lipase operon from mycoplasma mycoides subspecies mycoides lc.lipases, serine esterase enzymes, play an essential role in the mycoplasmal nutritional requirement for long-chain fatty acids. although the lipase(s) activity in different mycoplasma species has been investigated, the molecular biology of the corresponding genes has not been studied. using a single-primer pcr technique combined to more classical cloning systems, an operon containing three open reading frames (orf), each of which could encode a lipase protein of 264, 264 or 269 amino acids (aa), ...19957789792
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