| phenol hydroxylase from trichosporon cutaneum: gene cloning, sequence analysis, and functional expression in escherichia coli. | a cdna clone encoding phenol hydroxylase from the soil yeast trichosporon cutaneum was isolated and characterized. the clone was identified by hybridization screening of a bacteriophage lambda zap-based cdna library with an oligonucleotide probe which corresponded to the n-terminal amino acid sequence of the purified enzyme. the cdna encodes a protein consisting of 664 amino acids. amino acid sequences of a number of peptides obtained by edman degradation of various cleavage products of the puri ... | 1992 | 1429434 |
| the yeasts of cheese brines. | a total of 365 yeasts were isolated from the brines of soft, semihard and hard cheeses from different manufacturers. identification was based on 131 characteristics, primarily employing a method with microtitration plates. most brines exhibited a characteristic yeast flora. the predominant strains proved to be mainly debaryomyces hansenii and candida versatilis. in a few brines trichosporon beigelii, c. rugosa, c. intermedia, kluyveromyces marxianus, saccharomyces sp. and c. tenuis/polymorpha we ... | 1990 | 2282287 |
| biotransformation of polycyclic aromatic hydrocarbons by yeasts isolated from coastal sediments. | yeast abundance in the sediments of 13 coastal sites in massachusetts was quantified, and the potential of yeast isolates to biotransform polycyclic aromatic hydrocarbons (pahs) was determined. plate counts of yeasts varied between 10(2) to 10(7) cfu g (dry weight) of sediment-1. the most abundant genera isolated and identified included candida, cryptococcus, rhodotorula, torulopsis, and trichosporon. more than 50% of the isolates from heavily contaminated sites transformed phenanthrene, as dete ... | 1993 | 8517753 |
| purification and characterization of three extracellular protopectinases with polygalacturonase activities from trichosporon penicillatum. | in a culture filtrate of trichosporan penicillatum b2, which is a gamma-ray irradiation mutant induced from t. penicillatum sno3, we found three kinds of pectin-releasing enzymes, protopectinases se1, se2, and se3, that have endo-polygalacturonase activity. these enzymes were purified to homogeneity with cation-exchange and size exclusion chromatographies. the major ppase in the culture filtrate was ppase se1, which accounted for 75% of total activities in the culture filtrate, and the two other ... | 1996 | 8829524 |
| cloning of a protopectinase gene of trichosporon penicillatum and its expression in saccharomyces cerevisiae. | a protopectinase (ppase)-encoding gene, pse3, from trichosporon penicillatum was cloned by colony hybridization using two oligonucleotide probes synthesized from the n-terminal amino acid sequences of native ppase se1 and one peptide from a lysyl endopeptidase digest. nucleotide sequencing revealed that pse3 contains an orf encoding a 367 amino acid protein. mature ppase se3 is composed of 340 amino acids and the n-terminus of the orf appeared to correspond to a signal peptide and a propeptide p ... | 1997 | 9168614 |
| isolation and expression of the gene which encodes a novel enzyme with polymethoxygalacturonate-degrading activity in trichosporon penicillatum. | the novel gene named psx1, encoding a new protopectinase with the polymethoxygalacturonase activity, was isolated from trichosporon penicillatum. nucleotide sequencing revealed that the psx1 gene is composed of 1080 bases (360 amino acids, 38,747 da). the n-terminal amino acid sequences of the open reading frame correspond to a signal peptide and propeptide processed by a kex2-like proteinase. mature ppase sx1 was composed of 334 amino acids (36,121 da). ppase sx1 produced by a s. cerevisiae tra ... | 1997 | 9315736 |
| biodiversity of killer activity in yeasts isolated from the brazilian rain forest. | the occurrence of killer activity against a panel composed of 22 industrially and (or) medically important yeasts was investigated in 438 yeast and yeast-like cultures belonging to 96 species, isolated from different environments of the brazilian rain forest. altogether, 26% of ascomycetes, 56% of basidiomycetes, and 42% of yeast-like cultures exhibited killer activity against at least one of the panel yeasts. more than 15 species never reported before as toxin producers were found, with pseudoz ... | 2000 | 10932353 |
| crystallization and preliminary x-ray analysis of endopolygalacturonase se1 from trichosporon penicillatum. | endopolygalacturonase se1 from trichosporon penicillatum has been crystallized by the hanging-drop method of vapour diffusion using ammonium sulfate as a precipitant. the crystals belong to the hexagonal space group p6(1) or p6(5), with unit-cell parameters a = b = 135.0, c = 70.7 a, gamma = 120 degrees. the calculated v(m) based on one molecule per asymmetric unit is 3.09 a(3) da(-1). a native data set from a crystal has been collected to 2.0 a resolution on a cu kalpha rotating-anode x-ray sou ... | 2000 | 11092941 |
| crystallization and preliminary x-ray analysis of a novel pectolytic enzyme, polymethoxygalacturonase sx1 from trichosporon penicillatum. | a novel pectolytic enzyme, polymethoxygalacturonase sx1 from trichosporon penicillatum, with a molecular weight of 36 kda was crystallized by the hanging-drop vapour-diffusion method using polyethylene glycol 1000 as a precipitant. the crystals belonged to the monoclinic space group c2, with unit-cell parameters a = 165.6, b = 61.0, c = 48.7 a, beta = 93.1 degrees. the calculated v(m) based on one molecule per asymmetric unit was 3.40 a(3) da(-1). a native data set was collected to 2.08 a resolu ... | 2001 | 11223531 |
| trichosporon penicillatum sp.n. | | 1965 | 14315634 |
| microbial production of pectin from citrus peel. | a new method for the production of pectin from citrus peel was developed. for this purpose, a microorganism which produces a protopectin-solubilizing enzyme was isolated and identified as a variety of trichosporon penicillatum. the most suitable conditions for the pectin production were determined as follows. citrus (citrus unshiu) peel was suspended in water (1:2, wt/vol), the organism was added, and fermentation proceeded over 15 to 20 h at 30 degrees c. during the fermentation, the pectin in ... | 1980 | 16345556 |
| fungi isolated from the excreta of wild birds in screening centers in pelotas, rs, brazil. | the identification of the fungal species belonging to the healthy microflora in animals is a precondition for the recognition of pathological processes causing them. the aim of this study was to investigate the presence of potentially pathogenic fungi in the feces of wild birds collected in screening centers. samples were collected from the feces of 50 cages with different species of birds. the samples were processed according to the modified method staib and the plates incubated at 32 °c for up ... | 2017 | 25351548 |
| multicenter study evaluating the vitek ms system for identification of medically important yeasts. | the optimal management of fungal infections is correlated with timely organism identification. matrix-assisted laser desorption ionization-time of flight (maldi-tof) mass spectrometry (ms) is revolutionizing the identification of yeasts isolated from clinical specimens. we present a multicenter study assessing the performance of the vitek ms system (biomérieux) in identifying medically important yeasts. a collection of 852 isolates was tested, including 20 candida species (626 isolates, includin ... | 2013 | 23658267 |
| the use of fame analyses to discriminate between different strains of geotrichum klebahnii with different viabilities. | a considerable decline in viability of spray dried cells of geotrichum klebahnii was observed and was attributed to an undefined alteration of the used strain. as common techniques were not able to distinguish the altered from the still viable strains, we used the fatty acid methyl ester (fame) analysis. on the basis of fame data we were able to discriminate the three strains under investigation. especially the ratios of cis/trans fatty acid ratios and of saturated/unsaturated fatty acid were si ... | 2012 | 22806872 |