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identification and composition of the tonsillar and anal enterococcal and streptococcal flora of dogs and cats.enterococcus faecalis was the most frequently isolated enterococcal species from anal swabs and tonsils of dogs and cats, although in the anal samples from dogs ent. hirae was found almost as often as ent. faecalis. most ent. faecium strains from dog tonsils differed from those associated with humans and other animals in that they fermented sorbitol. typical ent. avium as well as atypical ent. avium-like strains were seen in dogs, while the related species ent. raffinosus was associated with cat ...19921447058
enterococcus columbae, a species from pigeon intestines.gram-positive cocci which dominate in the intestinal flora of domestic pigeons were found to constitute a new species of the genus enterococcus. the strains were most closely related to enterococcus cecorum, originally described as streptococcus cecorum, a carboxyphilic species from chicken intestines, and to enterococcus avium. the pigeon strains resemble e. cecorum and also many e. avium strains in their lack of group d antigen and in being more sensitive to nacl than other enterococci. the ty ...19902272492
enterococcus cecorum septicemia in a malnourished adult patient.enterococcus cecorum, a species typically isolated from chicken, pigs, calves, horses, ducks, cats, dogs, and canaries, was isolated from the blood of a patient with severe septicemia. the isolate was identified by conventional biochemical tests. identity as enterococcus cecorum was confirmed by sds-page analysis of whole cell protein. this is the first report of the isolation of enterococcus cecorum in a clinical setting.19979323472
humic acid reduction by propionibacterium freudenreichii and other fermenting bacteriairon-reducing bacteria have been reported to reduce humic acids and low-molecular-weight quinones with electrons from acetate or hydrogen oxidation. due to the rapid chemical reaction of amorphous ferric iron with the reduced reaction products, humic acids and low-molecular-weight redox mediators may play an important role in biological iron reduction. since many anaerobic bacteria that are not able to reduce amorphous ferric iron directly are known to transfer electrons to other external accept ...19989797315
sequencing the gene encoding manganese-dependent superoxide dismutase for rapid species identification of enterococci.simple pcr and sequencing assays that utilize a single pair of degenerate primers were used to characterize a 438-bp-long dna fragment internal (soda(int)) to the soda gene encoding the manganese-dependent superoxide dismutase in 19 enterococcal type strains (enterococcus avium, enterococcus casseliflavus, enterococcus cecorum, enterococcus columbae, enterococcus dispar, enterococcus durans, enterococcus faecalis, enterococcus faecium, enterococcus flavescens, enterococcus gallinarum, enterococc ...200010618129
recurrent bacteremic peritonitis caused by enterococcus cecorum in a patient with liver cirrhosis.enterococcus cecorum (formerly streptococcus cecorum), originally isolated from poultry intestines, has rarely been encountered in human diseases. a 60-year-old man with liver cirrhosis and hepatocellular carcinoma developed peritonitis on the seventh day of his hospitalization. cultures of one blood sample and one ascites fluid sample obtained on that day both grew e. cecorum. the patient received intravenous cefoxitin therapy and initially responded well. unfortunately, another episode of peri ...200010835030
continuous ambulatory peritoneal dialysis peritonitis due to enterococcus cecorum.enterococcus cecorum was isolated as the etiologic agent of a continuous ambulatory peritoneal dialysis peritonitis episode in an alcoholic patient. to date, this is only the third infection due to this bacterium, found in the intestinal tract of many domestic animals, that has been reported in humans.200010970419
identification of enterococcus species and phenotypically similar lactococcus and vagococcus species by reverse checkerboard hybridization to chaperonin 60 gene sequences.data from four recent studies (s. h. goh et al., j. clin. microbiol. 36:2164-2166, 1998; s. h. goh et al., j. clin. microbiol. 34:818-823, 1996; s. h. goh et al., j. clin. microbiol. 35:3116-3121, 1997; a. y. c. kwok et al., int. j. syst. bacteriol. 49:1181-1192, 1999) suggest that an approximately 600-bp region of the chaperonin 60 (cpn60) gene, amplified by pcr with a single pair of degenerate primers, has utility as a potentially universal target for bacterial identification (id). this cpn60 ...200011060051
evidence for horizontal gene transfer in evolution of elongation factor tu in enterococci.the elongation factor tu, encoded by tuf genes, is a gtp binding protein that plays a central role in protein synthesis. one to three tuf genes per genome are present, depending on the bacterial species. most low-g+c-content gram-positive bacteria carry only one tuf gene. we have designed degenerate pcr primers derived from consensus sequences of the tuf gene to amplify partial tuf sequences from 17 enterococcal species and other phylogenetically related species. the amplified dna fragments were ...200011092850
lactobacillus agilis is an important component of the pigeon crop flora.aims: to examine the presence of lactic acid bacteria (lab: enterococci, streptococci and lactobacilli) in the pigeon crop. methods and results: the crops of 10 pigeons were sampled and inoculated on agar plates for isolation of streptococci, enterococci and lactobacilli. the isolates were identified using tdna-pcr. lactobacillus agilis, a species described in 1981 from municipal sewage, was the dominant component in eight of these pigeon crop sacs. a lactobacillus species related to l. fermentu ...200111556914
isolation of enterococcus cecorum from bone lesions in broiler chickens. 200211822370
enterococcus cecorum empyema thoracis successfully treated with cefotaxime.we report the first case of enterococcus cecorum empyema thoracis and spontaneous bacterial peritonitis in a 44-year-old man with underlying cirrhosis. the patient responded to cefotaxime (mic, 0.25 microg/ml) treatment and drainage of the empyema. susceptibility of e. cecorum to expanded-spectrum cephalosporins could be due to its production of types of penicillin-binding proteins similar to those produced by streptococcus species rather than to those produced by enterococcus species (as predic ...200414766891
high prevalence of tetracycline resistance in enterococcus isolates from broilers carrying the erm(b) gene.a total of 73 isolates of enterococcus spp. carrying the erm(b) gene were obtained from cloacal swabs of broiler chickens derived from 13 different farms in belgium. the erm(b) gene encodes resistance to macrolides, lincosamides and streptogramin b antibiotics (mls(b)). the isolates belonged to eight different species: enterococcus avium (eight isolates), enterococcus casseliflavus (11 isolates), enterococcus cecorum (eight isolates), enterococcus durans (seven isolates), enterococcus faecalis ( ...200717899464
prokaryotic microbiota of recycled paper mills with low or zero effluent.the tendency in the paper industry is to close all water loops to save water. this leads to higher process temperatures and may increase the colloidal and dissolved material in the process circulation. increase of nutrients in the water circuits may favor microbiological growth and fouling. in this paper the chemical and microbial compositions of water circuits and deposits were studied of two closed cycled paper/board mills, one mill totally closed (0 m(3) waste water t(-1)), and the other low ...200818651188
molecular analysis of bacterial populations in the ileum of broiler chickens and comparison with bacteria in the cecum.bacterial populations in the ileum of broiler chickens were analyzed by molecular analysis of 16s rrna genes and compared to those in the cecum. bacteria found in the ileal mucosa were mainly gram-positive with low g+c content. there were 15 molecular species among 51 cloned sequences. more than 70% of the cloned sequences were related to lactobacilli and enterococcus cecorum. two sequences had 95% or less homology to existing database sequences. terminal restriction fragment length polymorphism ...200219709251
bacteremia caused by non-faecalis and non-faecium enterococcus species at a medical center in taiwan, 2000 to 2008.summary objectives: human infections due to non-faecalis and non-faecium enterococcus species are emerging but data on the characteristics of these infections are limited.201020433865
arthritis and osteomyelitis associated with enterococcus cecorum infection in broiler and broiler breeder chickens in ontario, canada.in august 2008, an ontario broiler chicken flock experienced an outbreak of lameness in 4-week-old birds, with morbidity reaching 7% by day 3. necropsy examination and histopathology revealed arthritis of the hock, stifle, and coxofemoral joints, and femoral and vertebral osteomyelitis. enterococcus cecorum was isolated from the lesions and identified by 16s ribosomal rna sequencing. in october 2008, a second case of e. cecorum osteomyelitis involved a flock of 9-week-old broiler breeder chicken ...201020622242
association of enterococcus cecorum with vertebral osteomyelitis and spondylolisthesis in broiler parent chicks.enterococcus cecorum is the most frequently occurring enterococcal species in the intestine of chickens of over 12 weeks of age, and there are few reports on its isolation from the skeleton of broiler parent chicks. in the present study, observations on vertebral osteomyelitis and spondylolisthesis ('kinky back syndrome') showing high incidence in 8 broiler parent flocks in different parts of hungary are summarised. clinical signs were seen only in roosters between 5 and 13 weeks of age. disease ...201121354938
experimental reproduction of enterococcal spondylitis in male broiler breeder chickens.there has been a recent emergence of epidemic spinal infections with necrosis causing lameness and mortality in male broilers and broiler breeders. mortality in affected flocks may be as high as 15%. the disease has been called enterococcal spondylitis (es), based on the frequent isolation of enterococcus cecorum from the lesions and necrosis and inflammation observed in the free thoracic vertebrae (ftv) of affected birds. male broiler breeders in an experimental setting were challenged with pur ...201121793445
review of antimicrobial therapy of selected bacterial diseases in broiler chickens in canada.this paper reviews common therapeutic applications of antimicrobials in broiler chicken production in relation to canadian guidelines, surveillance data, and emerging public health concerns about antimicrobial use (amu). escherichia coli, clostridium perfringens, and staphylococcus spp., were reviewed because of their animal health and economic significance. enterococcus cecorum and salmonella were included because of their importance in antimicrobial resistance (amr) surveillance. this review i ...201223729827
an outbreak and source investigation of enterococcal spondylitis in broilers caused by enterococcus cecorum.enterococcus cecorum was isolated from spondylitis lesions in broilers from two flocks in north carolina that were experiencing increased mortality. affected birds showed paresis and paralysis, clinical signs characteristic of enterococcal spondylitis (es). affected birds rested on their hocks and caudal abdomens with legs extended forward and were unable to stand or walk. necropsy examination of affected birds revealed firm to hard inflammatory masses involving the vertebral bodies at the level ...201223397855
investigation of microbial communities of chinese sourdoughs using culture-dependent and dgge approaches.sourdough is typically characterized by the complex microbial communities mainly comprising of yeasts and lactic acid bacteria (lab). the objective of this study was to explore the microbiota of chinese traditional sourdoughs collected from different areas of china using culture-dependent and denaturing gradient gel electrophoresis (dgge) methods. a total of 131 yeasts, 2 molds, and 106 lab strains were isolated and identified. based on the culture-dependent analysis, the populations of yeasts a ...201526461081
changes of poultry faecal microbiota associated with clostridium difficile colonisation.bacterial, fungal and archaeal microbiota was analysed in 143 chicken faecal samples from a single poultry farm. after dhplc (denaturing high performance liquid chromatography) 15 bacterial groups, 10 fungal groups and a single archaeal species were differentiated. samples were grouped into two clusters with significantly different frequencies of c. difficile positive and negative samples in each cluster. acidaminococcus intestini, described here for the first time as a part of poultry faecal mi ...201323664184
culture methods impact recovery of antibiotic-resistant enterococci including enterococcus cecorum from pre- and postharvest chicken.pathogenic strains of enterococcus cecorum (ec) expressing multidrug resistance have emerged. in national antimicrobial resistance monitoring system (narms) data, ec is rarely recovered from chickens. two narms methodologies (fda and usda) were compared with standard culture (sc) techniques for recovery of ec. narms methods failed to detect ec in 58 caecal samples, 20 chicken breast or six whole broiler samples. ec was recovered from 1 of 38 (2·6%) and 2 of 38 (5·2%) preharvest spinal lesions (u ...201727987523
multilevel selection of bcrabdr-mediated bacitracin resistance in enterococcus faecalis from chicken farms.in this study we isolated 109 enterococcus faecalis from chicken faecal samples in 6 provinces of china to investigate the prevalence and transmission mechanism of the bacitracin resistance locus bcrabdr in e. faecalis. thirty-seven bcrabdr-positive e. faecalis were detected with 26 different pfge clusters. the mlst of 14 positive strains belonged to st16 and we also detected three new sequence types. s1-pfge analysis indicated that the locus was located on plasmids presenting different sizes, w ...201627731342
enterococcus cecorum human infection, france.enterococcus cecorum is a bacterium of the intestinal tract of many domestic animals that is rarely reported as human pathogen. here we report the first case of incisional hernia plate infection and the first case of urinary tract colonization due to e. cecorum from patients in marseille, france.201526199733
draft genome sequences of five clinical enterococcus cecorum strains isolated from different poultry species in poland.here, we report five draft genome sequences of enterococcus cecorum strains that were isolated from different bird species of affected poultry flocks (commercial broilers [cb], broiler breeders [bb], commercial layers [cl], ducks [d], and geese [g]) in poland.201526383654
draft genome sequences of two commensal enterococcus cecorum strains isolated from chickens in belgium.here, we report the draft genome sequences of two commensal enterococcus cecorum strains (1710s23 and 1711s24), cultivated from the ceca of healthy laying hens originating from different farms in belgium.201526404586
enterococcus cecorum sepsis in a patient with liver cirrhosis, successfully treated with ceftriaxone.enterococcus cecorum septicemia is rare in humans. this case describes a man with underlying liver cirrhosis, a comorbidity that contributes to half of the e. cecorum infections described in the current literature. the patient was successfully treated with ceftriaxone. identification of this species was accurately made with matrix-assisted laser desorption-ionization time-of-flight mass spectrometry and confirmed by 16s rdna sequencing.201627112579
pathogenesis of enterococcal spondylitis caused by enterococcus cecorum in broiler chickens.enterococcal spondylitis (es) is a disease of commercial broiler chickens, with a worldwide distribution. symmetrical hind limb paralysis typical of es results from infection of the free thoracic vertebra (ftv) by pathogenic strains of enterococcus cecorum . to determine the pathogenesis of es, birds with natural and experimental es were studied over time. in natural disease, case birds (n = 150) from an affected farm and control birds (n = 100) from an unaffected farm were evaluated at weeks 1- ...201727511310
colonization patterns of enterococcus cecorum in two different broiler production cycles detected with a newly developed quantitative real-time pcr.although enterococcus cecorum (ec) infection is one of the most important bacterial diseases in modern broiler chickens today, many aspects of epidemiology and pathogenesis are still unknown. there is a need for better detection methods for ec than classical cultivation. in the present study, we describe the validation and application of a newly developed quantitative taqman real-time pcr (qpcr) assay based on the 16s-rrna-gene for the detection of ec.201728476097
phenotypic and genotypic characterization of enterococcus cecorum strains associated with infections in poultry.from the beginning of the 21(st) century enterococcus cecorum has emerged as a significant health problem for poultry raised under intensive production systems. to obtain new insights into this bacterial species, we investigated 82 clinical isolates originating from different poultry flocks in poland between 2011 and 2014.201627350248
comparison of pathogenic and non-pathogenic enterococcus cecorum strains from different animal species.enterococcus cecorum (ec) infection currently is one of the most important bacterial diseases of modern broiler chickens but can also affect ducks or other avian species. however, little is known concerning pathogenesis of ec and most studies concentrate on examinations of ec strains from broilers only. the objective of this study was to compare pathogenic and commensal ec strains from different animal species concerning different phenotypic and genotypic traits.201728193172
enterococcus cecorum infection in a racing pigeon.until now, enterococcus cecorum (ec) has been known as a pathogen for broilers, broiler breeders, and pekin ducks. in the present report, we describe a fatal systemic ec infection in a young racing pigeon (columba livia forma domestica). ec was isolated from the heart, liver, spleen, and intestine of the bird in pure culture. in the pathologic examination, the pigeon showed enteritis and an ulcerative gastritis, which may have been predisposing factors for the development of the generalized ec i ...201425619014
experimental reproduction of an enterococcus cecorum infection in pekin ducks.enterococcus cecorum (ec) was thus far only known as a pathogen for broilers and broiler breeders. recently there was evidence of ec field outbreaks in pekin duck flocks in germany. in this study we experimentally reproduced an ec infection in pekin ducks. at 12 days post hatch, groups of pekin ducks were infected orally, via the thoracic air sac or intravenously with 1.5 × 10(9) colony-forming units (cfu) of ec per bird or via the air sac with 8.5 × 10(5) or 8.5 × 10(7) cfu per bird. ducks of t ...201324164051
[relevance and diagnostics of selected bacterial pathogens of poultry].this paper provides an overview of diseases caused by bordetella avium, gallibacterium anatis, ornithobacterium rhinotracheale, riemerella anatipestifer and enterococcus cecorum in poultry flocks. these bacterial species are almost exclusively found in birds. their identification with biochemical methods is described and alternative molecular biological methods are discussed.201222526722
diversity of enterococcus cecorum from chickens.enterococcus cecorum is a normal inhabitant of the intestine of birds and other vertebrates. it has recently emerged in canada and other countries as an important cause of arthritis and osteomyelitis in chickens. the objectives of this study were to assess if this emergence was caused by a particular clone of e. cecorum and to assess the antimicrobial susceptibility of this organism. one hundred and thirteen e. cecorum isolates from infections in canadian chickens (cases) and from the ceca of co ...201222266160
enterococcus cecorum infections in broiler breeders and their offspring: molecular epidemiology.increased mortality and problems with lameness were reported in dutch broiler flocks from the year 2008 onwards. therefore, a field inventory, including 10 affected broiler flocks, nine corresponding broiler breeder flocks and five hatcheries, was carried out. the onset of clinical signs (lameness and increased mortality) started at about 2 weeks of age. the flock mortality varied from 3.1 to 8.1% at slaughter. post-mortem lesions of broiler flocks were characterized by the occurrence of pericar ...201122107095
isolation and characterization of vana genotype vancomycin-resistant enterococcus cecorum from retail poultry in japan.the isolation rate of high-level vancomycin-resistant enterococci (vre) from poultry samples in japan has increased in recent years. as this raises concerns for the potential spread of genes encoding vancomycin resistance, poultry is routinely screened for vre. here, we report the isolation and characterization of a vana genotype vancomycin-resistant enterococcus cecorum strain (e. cecorum ipha84) from retail domestic poultry in september 2009. the species identification was performed by biochem ...201122192623
enterococcus cecorum aortic valve endocarditis.we report a case of human aortic valve infective endocarditis caused by enterococcus cecorum. the organism was recovered from blood and identified by 16s ribosomal gene polymerase chain reaction (pcr). to the best of our knowledge, this is the first documented case of e. cecorum endocarditis. we wish to highlight the use of pcr for organism identification and to describe the initial misidentification of the pathogen.201121767709
molecular epidemiology of enterococcus cecorum isolates recovered from enterococcal spondylitis outbreaks in the southeastern united states.enterococcus cecorum, a normal intestinal inhabitant, is increasingly responsible for outbreaks of arthritis and osteomyelitis in chickens worldwide. enterococcal spondylitis (es) is a specific manifestation of e. cecorum-associated disease in which increased flock morbidity and mortality result from chronic infection involving the free thoracic vertebra. in this study the genetic relatedness and antimicrobial resistance of isolates recovered from es-affected flocks in the southeastern united st ...201222978557
fingerprinting of poultry isolates of enterococcus cecorum using three molecular typing methods.enterococcus cecorum is an emerging challenge to the broiler industry. the organism has been implicated in septicemia, spondylitis, arthritis, and osteomyelitis in commercial broilers and broiler breeders, which lead to economic losses attributed to increased mortality and culling rates, decreased average processing weights, and increased feed conversion ratios. the current study evaluated the genetic variability of 30 clinical isolates of e. cecorum from outbreaks in pennsylvania, using 3 molec ...201223104952
[infection with the capnophilic bacteria enterococcus cecorum in broiler chickens]. 201424867243
antimicrobial resistance, virulence determinants and genetic profiles of clinical and nonclinical enterococcus cecorum from poultry.enterococcus cecorum has been implicated as a possible cause of disease in poultry. however, the characteristics that contribute to pathogenesis of ent. cecorum in poultry have not been defined. in this study, ent. cecorum from carcass rinsates (n = 75) and diseased broilers and broiler breeders (n = 30) were compared based upon antimicrobial resistance phenotype, the presence of virulence determinants and genetic relatedness using pulsed-field gel electrophoresis (pfge). of the 16 antimicrobial ...201525470229
comprehensive report of an enterococcus cecorum infection in a broiler flock in northern germany.enterococcus cecorum is considered as an emerging pathogen in poultry and can cause substantial losses in broiler flocks. femoral head necrosis and spondylitis were described as the main pathological changes in infected chickens. nevertheless, little is known about the pathogenesis of enterococcus cecorum infection in broilers. this report shows for the first time the whole course of disease over an entire growing period including repeated necropsies and subsequent microbiological investigations ...201425539747
enterococcal-related vertebral osteoarthritis in south african broiler breeders: a case report.infections in broilers and broiler breeders by enterococcus cecorum, causing clinical disease, have increasingly been described in various countries in the northern hemisphere over the past decade. this case report describes an outbreak of enterococcal-associated vertebral osteoarthritis (evoa) in male broiler breeders in several flocks in south africa. male birds aged 4 and 9 weeks displayed the common presentation of lameness, paresis or complete paralysis. autopsies of culled birds revealed m ...201425686375
comparative genomic analysis identifies divergent genomic features of pathogenic enterococcus cecorum including a type ic crispr-cas system, a capsule locus, an epa-like locus, and putative host tissue binding proteins.enterococcus cecorum (ec) is the dominant enteric commensal of adult chickens and contributes to the gut consortia of many avian and mammalian species. while ec infection is an uncommon zoonosis, like other enterococcal species it can cause life-threating nosocomial infection in people. in contrast to other enterococci which are considered opportunistic pathogens, emerging pathogenic strains of ec cause outbreaks of musculoskeletal disease in broiler chickens. typical morbidity and mortality is ...201525860249
nosocomial infection due to enterococcus cecorum identified by maldi-tof ms and vitek 2 from a blood culture of a septic patient.we report the case of a nosocomial infection due to enterococcus cecorum isolated from a blood culture of a 75-year-old septic male patient. matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (maldi-tof ms) and vitek 2 succeeded in identification of the isolate.201526185687
a chicken embryo lethality assay for pathogenic enterococcus cecorum.pathogenic strains of enterococcus cecorum cause outbreaks of arthritis and osteomyelitis in chickens worldwide. enterococcal spondylitis (es) is a specific manifestation of e. cecorum-associated disease of broilers and broiler breeders characterized by increased flock mortality, resulting from unresolved infection of the free thoracic vertebra by pathogenic e. cecorum. a study of 22 es outbreaks in the southeast united states revealed that pathogenic e. cecorum strains isolated from spinal lesi ...201425055628
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