| aflp fingerprint analysis of bradyrhizobium strains isolated from faidherbia albida and aeschynomene species. | the diversity of bradyrhizobium isolates from faidherbia albida and aeschynomenee species was assessed using aflp analysis, a high-resolution genomic fingerprinting technique. reference strains from bradyrhizobium japonicum, bradyrhizobium elkanii and bradyrhizobium liaoningense were included for comparison. at a similarity level of 50%, a total of 34 different groups were obtained by cluster analysis of the genomic fingerprints. four of these clusters correspond to the three reference species, ... | 2000 | 10879988 |
| bradyrhizobium elkanii, bradyrhizobium yuanmingense and bradyrhizobium japonicum are the main rhizobia associated with vigna unguiculata and vigna radiata in the subtropical region of china. | cowpea (vigna unguiculata) and mung bean (vigna radiata) are important legume crops yet their rhizobia have not been well characterized. in the present study, 62 rhizobial strains isolated from the root nodules of these plants grown in the subtropical region of china were analyzed via a polyphasic approach. the results showed that 90% of the analyzed strains belonged to or were related to bradyrhizobium japonicum, bradyrhizobium liaoningense, bradyrhizobium yuanmingense and bradyrhizobium elkani ... | 2008 | 18564340 |
| phylogeny of bradyrhizobia from chinese cowpea miscellany inferred from 16s rrna, atpd, glnii, and 16s-23s intergenic spacer sequences. | the cowpea (vigna unguiculata l.), peanut (arachis hypogaea l.), and mung bean (vigna radiata l.) belong to a group of plants known as the "cowpea miscellany" plants, which are widely cultivated throughout the tropic and subtropical zones of africa and asia. however, the phylogeny of the rhizobial strains that nodulate these plants is poorly understood. previous studies have isolated a diversity of rhizobial strains from cowpea miscellany hosts and have suggested that, phylogenetically, they are ... | 2011 | 21491983 |
| bradyrhizobium huanghuaihaiense sp. nov., an effective symbiotic bacterium isolated from soybean (glycine max l.) nodules in northern (huang-huai-hai) plain of china. | in a survey of biodiversity and biogeography of rhizobia associated with soybean (glycine max l.) in different sites of northern (huang-huai-hai) plain of china, ten strains were defined as a novel genomic species in the genus of bradyrhizobium. they were distinguished from defined species in rflp (restriction fragment length polymorphism) of 16s rrna gene and intergenic spacer (igs) between 16s and 23s rrna genes. in box-pcr, these strains presented two patterns sharing 94% similarities, demons ... | 2011 | 22003042 |
| bradyrhizobium ottawaense sp. nov., a symbiotic nitrogen fixing bacterium from root nodules of soybeans in canada. | sixteen strains of symbiotic bacteria from root nodules of glycine max grown in ottawa, canada, were previously characterized and placed in a novel group within the genus bradyrhizobium. to verify their taxonomic status, these strains were further characterized using a polyphasic approach. all strains possessed identical 16s rrna gene sequences that were 99.79 % similar to the closest relative, bradyrhizobium liaoningense lmg 18230(t). phylogenetic analysis of concatenated atpd, glnii, reca, gyr ... | 2014 | 24969302 |
| genetic divergence of bradyrhizobium strains nodulating soybeans as revealed by multilocus sequence analysis of genes inside and outside the symbiosis island. | the genus bradyrhizobium has been considered to be a taxonomically difficult group. in this study, phylogenetics and evolutionary genetics analyses were used to investigate divergence levels among bradyrhizobium strains nodulating soybeans in china. eleven genospecies were identified by sequence analysis of three phylogenetic and taxonomic markers (smc00019, thra, and trua). this was also supported by analyses of eight genes outside the symbiosis island ("off-island" genes; smc00019, thra, trua, ... | 2014 | 24632260 |
| genetic characterization of soybean rhizobia isolated from different ecological zones in north-eastern afghanistan. | seventy rhizobial isolates were obtained from the root nodules of two soybean (glycine max) cultivars: japanese cultivar enrei and usa cultivar stine3300, which were inoculated with different soil samples from afghanistan. in order to study the genetic properties of the isolates, the dna sequences of the 16s rrna gene and symbiotic genes (nodd1 and nifd) were elucidated. furthermore, the isolates were inoculated into the roots of two soybean cultivars, and root nodule numbers and nitrogen fixati ... | 2017 | 28321006 |
| phylogenetic multilocus sequence analysis of indigenous slow-growing rhizobia nodulating cowpea (vigna unguiculata l.) in greece. | cowpea (vigna unguiculata) is a promiscuous grain legume, capable of establishing efficient symbiosis with diverse symbiotic bacteria, mainly slow-growing rhizobial species belonging to the genus bradyrhizobium. although much research has been done on cowpea-nodulating bacteria in various countries around the world, little is known about the genetic and symbiotic diversity of indigenous cowpea rhizobia in european soils. in the present study, the genetic and symbiotic diversity of indigenous rhi ... | 2017 | 28214058 |
| analysis of cellular fatty acids and phenotypic relationships of agrobacterium, bradyrhizobium, mesorhizobium, rhizobium and sinorhizobium species using the sherlock microbial identification system. | previous studies have demonstrated that cellular fatty acid analysis is a useful tool for identifying unknown strains of rhizobia and establishing taxonomic relationships between the species. in this study, the fatty acid profiles of over 600 strains belonging to the genera agrobacterium, bradyrhizobium, mesorhizobium, rhizobium and sinorhizobium were evaluated using the gaschromatography-based sherlock microbial identification system (mis). data collected with the mis showed that the three phyl ... | 2000 | 10758890 |
| bradyrhizobium liaoningense sp. nov., isolated from the root nodules of soybeans. | seventeen strains of extra-slowly growing (esg) soybean rhizobia isolated from root nodules of glycine soja and glycine max growing in five provinces (liaoning, heilongjiang, shanxi, hubei, and anhui) in the people's republic of china were compared with 48 reference strains belonging to the genera bradyrhizobium, rhizobium, and agrobacterium by performing a numerical analysis of 191 phenotypic features. our results showed that all of the esg strains examined clustered closely in the genus bradyr ... | 1995 | 7547289 |
| diversity and biogeography of rhizobia isolated from root nodules of glycine max grown in hebei province, china. | a total of 215 rhizobial strains were isolated and analyzed with 16s rrna gene, 16s-23s intergenic spacer, housekeeping genes atpd, reca, and glnii, and symbiotic genes nifh and nodc to understand the genetic diversity of soybean rhizobia in hebei province, china. all the strains except one were symbiotic bacteria classified into nine genospecies in the genera of bradyrhizobium and sinorhizobium. surveys on the distribution of these rhizobia in different regions showed that bradyrhizobium japoni ... | 2011 | 21340735 |
| genetic diversity of nodulating and non-nodulating rhizobia associated with wild soybean (glycine soja sieb. & zucc.) in different ecoregions of china. | a total of 99 bacterial isolates that originated from root nodules of glycine soja were characterized with restriction analyses of amplified 16s ribosomal dna and 16s-23s rdna intergenic spacers (its), and sequence analyses of 16s rrna, rpob, atpd, reca and nodc genes. when tested for nodulation of g. soja, 72 of the isolates were effective symbionts, and these belonged to five species: bradyrhizobium japonicum, bradyrhizobium elkanii, bradyrhizobium yuanmingense, bradyrhizobium liaoningense and ... | 2011 | 21303397 |
| genetic diversity and distribution of bradyrhizobium and azorhizobium strains associated with the herb legume zornia glochidiata sampled from across senegal. | herb legumes have great potential for rehabilitation of semi-arid degraded soils in sahelian ecosystems as they establish mutualistic symbiosis with n(2)-fixing rhizobia. a phylogenetic analysis was performed for 78 root nodule bacteria associated with the common sahelian herb legume zornia glochidiata reichb ex dc in senegal. based on its (rdna16s-23s) and reca sequences, these strains were shown to belong to the two genera bradyrhizobium and azorhizobium. strains of this latter, although frequ ... | 2009 | 19493641 |
| evidence from internally transcribed spacer sequence analysis of soybean strains that extant bradyrhizobium spp. are likely the products of reticulate evolutionary events. | the internally transcribed spacer (its) sequences of several members within each of 17 soybean bradyrhizobial serogroups were determined to establish whether the regions within all members of each serogroup were identical. the rationale was to provide a sequence-based alternative to serology. the objective also was to link the extensive older literature on soybean symbiosis based on serology with its sequence data for more recent isolates from both soybean and other legumes nodulated by rhizobia ... | 2009 | 19011081 |
| genetic diversity of native bradyrhizobia isolated from soybeans (glycine max l.) in different agricultural-ecological-climatic regions of india. | fifty isolates from root nodules of soybean plants sampled in five agricultural-ecological-climatic regions of india were analyzed by pcr-restriction fragment length polymorphism analysis of the 16s rrna gene, the intergenic spacer region between the 16s and 23s rrna genes (igs), and the nifh and nodc genes. eight haplotypes assigned to the bradyrhizobium genus were identified, and the genetic diversity was conserved across regions. sequence analyses of the igs and the dnak, glnii, reca, and nif ... | 2008 | 18676699 |
| bradyrhizobium betae sp. nov., isolated from roots of beta vulgaris affected by tumour-like deformations. | some varieties of sugar beet, beta vulgaris, cultivated in northern spain have large deformations that resemble the tumours produced by agrobacterium species. in an attempt to isolate the agent responsible for these deformations, several endophytic slow-growing bacterial strains were isolated, the macroscopic morphology of which resembled that of bradyrhizobium species. these strains were not able to produce tumours in nicotiana tabacum plants and, based on phylogenetic analysis of their 16s rrn ... | 2004 | 15280302 |
| dna-dna hybridization study of bradyrhizobium strains. | dna-dna hybridizations were performed between bradyrhizobium strains, isolated mainly from faidherbia albida and aeschynomene species, as well as bradyrhizobium reference strains. results indicated that the genus bradyrhizobium consists of at least 11 genospecies, i to xi. the genospecies formed four subgeneric groups that were more closely related to each other (>40% dna hybridization) than to other genospecies (<40% dna hybridization): (i) genospecies i (bradyrhizobium japonicum), iii (bradyrh ... | 2001 | 11491327 |
| specific detection of bradyrhizobium and rhizobium strains colonizing rice (oryza sativa) roots by 16s-23s ribosomal dna intergenic spacer-targeted pcr. | in addition to forming symbiotic nodules on legumes, rhizobial strains are members of soil or rhizosphere communities or occur as endophytes, e.g., in rice. two rhizobial strains which have been isolated from root nodules of the aquatic legumes aeschynomene fluminensis (irbg271) and sesbania aculeata (irbg74) were previously found to promote rice growth. in addition to analyzing their phylogenetic positions, we assessed the suitability of the 16s-23s ribosomal dna (rdna) intergenic spacer (igs) ... | 2001 | 11472944 |
| comparison of sequence analysis of 16s-23s rdna spacer regions, aflp analysis and dna-dna hybridizations in bradyrhizobium. | the sequences of the 16s-23s rdna intergenic spacer region of 62 strains of bradyrhizobium, including representatives of the three valid species, were determined. the majority of strains had a single rrna operon type and all contained a trna(ala) and a trna(ile) gene. analysis of the sequence data produced groupings in line with previously obtained aflp data. dna-dna hybridizations were performed to determine to what extent spacer sequences and aflp profiles reflected the overall genomic similar ... | 2001 | 11321108 |
| phylogenetic and dna-dna hybridization analyses of bradyrhizobium species. | the 16s rdna sequence of bradyrhizobium liaoningense was determined and analysed together with sequences of other bradyrhizobium species and related taxa. in addition, dna-dna hybridizations were performed between representative strains of the three bradyrhizobium species. bradyrhizobium liaoningense is genotypically highly related to bradyrhizobium japonicum, whereas bradyrhizobium elkanii is more distantly related to these two species. the fact that afipia, agromonas, blastobacter, nitrobacter ... | 2001 | 11211247 |
| evolutionary relationships among the soybean bradyrhizobia reconstructed from 16s rrna gene and internally transcribed spacer region sequence divergence. | from sequence divergence of 16s rrna genes and the internally transcribed spacer (its) region it is reported that variation in phylogenetic placement exists among the 17 different serotype strains of bradyrhizobium that have been isolated from nodules of soybean. evolutionary relationships among the bradyrhizobia were more resolved using reconstructions derived from its than from 16s rrna gene sequence divergence. strain usda 129 was placed together with usda 62, 110, 122 and 126, but did not cl ... | 2000 | 11155993 |
| pongamia pinnata inoculated with bradyrhizobium liaoningense pzhk1 shows potential for phytoremediation of mine tailings. | mine tailings contain high concentrations of metal contaminants and only little nutrients, making the tailings barren for decades after the mining has been terminated. effective phytoremediation of mine tailings calls for deep-rooted, metal accumulating, and soil fertility increasing plants with tolerance against harsh environmental conditions. we assessed the potential of the biofuel leguminous tree pongamia pinnata inoculated with plant growth promoting rhizobia to remediate iron-vanadium-tita ... | 2017 | 27858136 |
| bradyrhizobium arachidis sp. nov., isolated from effective nodules of arachis hypogaea grown in china. | twenty-three bacterial strains isolated from root nodules of arachis hypogaea and lablab purpureus grown in five provinces of china were classified as a novel group within the genus bradyrhizobium by analyses of pcr-based rflp of the 16s rrna gene and 16s-23s igs. to determine their taxonomic position, four representative strains were further characterized. the comparative sequence analyses of 16s rrna and six housekeeping genes clustered the four strains into a distinctive group closely related ... | 2013 | 23295123 |
| bradyrhizobium daqingense sp. nov., isolated from soybean nodules. | thirteen slow-growing rhizobial strains isolated from root nodules of soybean (glycine max l.) grown in daqing city in china were classified in the genus bradyrhizobium based on 16s rrna gene sequence analysis. multilocus sequence analysis of igs, atpd, glnii and reca genes revealed that the isolates represented a novel clade in this genus. dna-dna relatedness lower than 42.5 % between the representative strain ccbau 15774(t) and the type strains of the closely related species bradyrhizobium lia ... | 2013 | 22544787 |
| functional characterization of a csor-cuea divergon in bradyrhizobium liaoningense ccnwsx0360, involved in copper, zinc and cadmium cotolerance. | random mutagenesis in a symbiotic nitrogen-fixing bradyrhizobium liaoningense ccnwsx0360 (bln0360) using tn5 identified five copper (cu) resistance-related genes. they were functionally sorted into three groups: transmembrane transport (cuea and tolc); oxidation (copa); and protection of the membrane barrier (lpte and ctpa). the gene cuea, together with the upstream csor (cu-sensitive operon repressor), constituted a csor-cuea divergon which plays a crucial role in cu homeostasis. deletion of cu ... | 2016 | 27725778 |
| nodulation characterization and proteomic profiling of bradyrhizobium liaoningense ccbau05525 in response to water-soluble humic materials. | the lignite biodegradation procedure to produce water-soluble humic materials (wshm) with a penicillium stain was established by previous studies in our laboratory. this study researched the effects of wshm on the growth of bradyrhizobium liaoningense ccbau05525 and its nodulation on soybean. results showed that wshm enhanced the cell density of ccbau05525 in culture, and increased the nodule number, nodule fresh weight and nitrogenase activity of the inoculated soybean plants. then the chemical ... | 2015 | 26054030 |
| [biodiversity of rhizobia associated with acacia melanoxylon grown in south china]. | this study aimed to investigate the genetic diversity of 174 isolates of symbiotic bacteria associated with acacia melanoxylon obtained from 15 sampling sites in guangdong, fujian and jiangxi provinces of china. | 2012 | 23457793 |
| characterization of cyclic ╬▓-glucans of bradyrhizobium by maldi-tof mass spectrometry. | periplasmic, cyclic ╬▓-glucans isolated from bradyrhizobium elkanii, bradyrhizobium liaoningense, and bradyrhizobium yuanmingense strains have been investigated by means of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof ms), 1d and 2d nuclear magnetic resonance (nmr), as well as standard chemical methods. these compounds are built of 10-13 d-glucose residues. the main fractions contain molecules assembled of 12 hexose units (m(w)=1945.363da). glucose mono ... | 2011 | 21665197 |