| taxonomic study of the genus brachybacterium: brachybacterium nesterenkovii sp. nov. | a new species, brachybacterium nesterenkovii, is proposed for a group of coryneform bacteria that were isolated from milk products. these organisms have morphological, biochemical, and chemotaxonomic characteristics that are peculiar to the genus brachybacterium. in contrast to strains of the only previously described species of the genus, brachybacterium faecium, the representatives of the new species lack glycine in their peptidoglycan, although the peptidoglycan is of the same general type, a ... | 1992 | 1736971 |
| characteristics of cdc group 3 and group 5 coryneform bacteria isolated from clinical specimens and assignment to the genus dermabacter. | over a 1-year period, 11 isolates (including 5 from blood cultures) of the recently described cdc group 3 and group 5 coryneform bacteria were derived from clinical specimens and compared with reference strains. biochemical characteristics indicated a very close relationship between cdc group 3 and group 5 coryneform bacteria. the ability of cdc group 3 and the inability of cdc group 5 coryneform bacteria to ferment xylose were the only reactions that were different for the two taxa. chemotaxono ... | 1994 | 8051248 |
| arthrobacter, brachybacterium and planococcus isolates identified from antarctic sea ice brine. description of planococcus mcmeekinii, sp. nov. | three facultative psychrophilic gram-positive bacterial strains were isolated from brine samples from the sea ice community in antarctica. all strains were coccoid to rod-shaped and exhibited broad salinity and temperature ranges for growth. the three strains were subjected to 16s rdna sequencing and subsequent phylogenetic analysis. all possess unique 16s rdna sequences indicating they are new, previously unreported organisms. phylogenetic analyses coupled with phenotypic characterization indic ... | 1998 | 9704115 |
| novel actinobacteria from marine sponges. | actinobacteria exclusively within the sub-class acidimicrobidae were shown by 16s rdna community analysis to be major components of the bacterial community associated with two sponge species in the genus xestospongia. four groups of actinobacteria were identified in xestospongia spp., with three of these four groups being found in both xestospongia muta from key largo, florida and xestospongia testudinaria from manado, indonesia. this suggests that these groups are true symbionts in these sponge ... | 2005 | 15726288 |
| biochemical evidence for the proteolytic degradation of infectious prion protein prpsc in hamster brain homogenates by foodborne bacteria. | prp(sc) is a general term to describe the infectious agent causing transmissible spongiform encephalopathy (tse), and the protease-resistant form of cellular prp(c). in this study, we have identified several protease-secreting bacteria able to degrade prp(sc) under more or less native conditions (30 degrees c, ph 8), focusing on strains isolated mainly from cheese. one hundred and ninty-nine protease-secreting isolates belonging to the actinomycetales and bacillales were screened for the express ... | 2006 | 16464698 |
| rapid analysis of two food-borne microbial communities at the species level by fourier-transform infrared microspectroscopy. | the species composition of microbial communities in natural habitats may be extremely complex and therefore a quantitative analysis of the fraction each species contributes to the consortium has proven to be difficult. during recent years, the identification of bacterial pure cultures based on their infrared spectra has been established. fourier-transform infrared microspectroscopy now proceeds a step further and allows identification of microorganisms directly plated from community dilutions. i ... | 2006 | 16623742 |
| biodiversity of bacterial ecosystems in traditional egyptian domiati cheese. | bacterial biodiversity occurring in traditional egyptian soft domiati cheese was studied by pcr-temporal temperature gel electrophoresis (ttge) and pcr-denaturing gradient gel electrophoresis (dgge). bands were identified using a reference species database (j.-c. ogier et al., appl. environ. microbiol. 70:5628-5643, 2004); de novo bands having nonidentified migration patterns were identified by dna sequencing. results reveal a novel bacterial profile and extensive bacterial biodiversity in domia ... | 2007 | 17189434 |
| stability of microbial communities in goat milk during a lactation year: molecular approaches. | the microbial communities in milks from one herd were evaluated during 1-year of lactation, using molecular methods to evaluate their stability and the effect of breeding conditions on their composition. the diversity of microbial communities was measured using two approaches: molecular identification by 16s and 18s rdna sequencing of isolates from counting media (two milks), and direct identification using 16s rdna from clone libraries (six milks). the stability of these communities was evaluat ... | 2007 | 17604934 |
| purification and characterization of a maltooligosaccharide-forming amylase that improves product selectivity in water-miscible organic solvents, from dimethylsulfoxide-tolerant brachybacterium sp. strain lb25. | a bacterium that secretes maltooligosaccharide-forming amylase in a medium containing 12.5% (vol/vol) dimethylsulfoxide (dmso) was isolated and identified as brachybacterium sp. strain lb25. the amylase of the strain was purified from the culture supernatant, and its molecular mass was 60 kda. the enzyme was stable at ph 7.0-8.5 and active at ph 6.0-7.5. the optimum temperature at ph 7.0 was 35 degrees c in the presence of 5 mm cacl(2). the enzyme hydrolyzed starch to produce maltotriose primari ... | 2007 | 17619813 |
| production of volatile aroma compounds by bacterial strains isolated from different surface-ripened french cheeses. | twelve bacterial strains belonging to eight taxonomic groups: brevibacterium linens, microbacterium foliorum, arthrobacter arilaitensis, staphylococcus cohnii, staphylococcus equorum, brachybacterium sp., proteus vulgaris and psychrobacter sp., isolated from different surface-ripened french cheeses, were investigated for their abilities to generate volatile aroma compounds. out of 104 volatile compounds, 54 volatile compounds (identified using dynamic headspace technique coupled with gas chromat ... | 2007 | 17701035 |
| cultivable bacteria associated with larval gut of prothiofos-resistant, prothiofos-susceptible and field-caught populations of diamondback moth, plutella xylostella and their potential for, antagonism towards entomopathogenic fungi and host insect nutrition. | to evaluate whether the gut bacteria of insecticide-resistant, insecticide-susceptible and field-caught populations of the lepidopteran insect pest diamondback moth (dbm)--plutella xylostella (l.)--are variable and their role in host protection and nutrition. | 2007 | 17973916 |
| identities of arthrobacter spp. and arthrobacter-like bacteria encountered in human clinical specimens. | after the initial description of arthrobacter spp. isolated from clinical specimens in the mid-1990s, very few further reports on arthrobacter spp. have appeared in the clinical microbiology literature. the aim of the present study was to elucidate the distribution of arthrobacter spp. and arthrobacter-like bacteria encountered in clinical specimens by studying 50 consecutively isolated or received strains of large-colony-forming, whiteish-grayish, non-cheese-like-smelling, nonfermentative gram- ... | 2008 | 18650355 |
| a maltooligosaccharide-forming amylase gene from brachybacterium sp. strain lb25: cloning and expression in escherichia coli. | brachybacterium sp. strain lb25 produces a maltooligosaccharide-forming amylase that improves product selectivity in water-miscible organic solvents. the enzyme hydrolyzed starch to produce maltotriose primarily. the structural gene encoding the amylase from strain lb25 was cloned and sequenced. the amino acid sequence of the product showed significant similarity (45 to 49%) to amylases from the genus streptomyces. the amylase gene was expressed in escherichia coli, but the specific activity of ... | 2008 | 18776669 |
| cross-utilization and expression of outer membrane receptor proteins for siderophore uptake by diamondback moth plutella xylostella (lepidoptera: plutellidae) gut bacteria. | siderophore production by entomo- and phytopathogens, plus the cross-utilization of these siderophores and expression of outer membrane receptor proteins (omrps) by diamondback moth (dbm) gut bacterial strains, were all examined. all the tested strains grew in the presence of 2, 2'-dipyridyl, and the brachybacterium sp. psgb10, pseudomonas sp. prgb06, and serratia marcescens flgb16 strains were found to cross-utilize the siderophores of various entomopathogens, including bacillus thuringiensis. ... | 2008 | 19054090 |
| [phylogenetic diversity of cultivable bacteria associated with a sea anemone from coast of the naozhou island in zhanjiang, china]. | to understand the diversity of cultivable bacteria isolated from a sea anemone collected from the coastal water of the naozbou island in the leizhou bay on south china sea. | 2009 | 19445182 |
| molecular identification of airborne bacteria associated with aerial spraying of bovine slurry waste employing 16s rrna gene pcr and gene sequencing techniques. | polymerase chain reaction amplification of the universal 16s ribosomal rna (rrna) gene was performed on a collection of 38 bacterial isolates, originating from air sampled immediately adjacent to the agricultural spreading of bovine slurry. a total of 16 bacterial genera were identified including both gram-positive and gram-negative genera. gram-positive organisms accounted for 34/38 (89.5%) of total bacterial numbers consisting of 12 genera and included staphylococcus (most common genus isolate ... | 2010 | 20018377 |
| a polyphasic study on the species diversity of the sediment microbiota of lake hévíz. | lake hévíz is the largest natural thermal lake of europe, harboring special bacterial communities. the aim of the present study was to gain information about the distribution and species diversity of the sediment microbiota, with special focus on actinobacteria, by using cultivation-based and -independent molecular methods. samples from two depths were taken in two different locations in october 2007. 245 strains were isolated, grouped to 85 otus by ardra, and identified by 16s rdna sequencing. ... | 2009 | 20038486 |
| isolation and characterization of bacteria resistant to metallic copper surfaces. | metallic copper alloys have recently attracted attention as a new antimicrobial weapon for areas where surface hygiene is paramount. currently it is not understood on a molecular level how metallic copper kills microbes, but previous studies have demonstrated that a wide variety of bacteria, including escherichia coli, staphylococcus aureus, and clostridium difficile, are inactivated within minutes or a few hours of exposure. in this study, we show that bacteria isolated from copper alloy coins ... | 2010 | 20048058 |
| [diversity of culturable bacteria associated with the sea urchin hemicentrotus pulcherrimus from naozhou island]. | to investigate the diversity of culturable bacteria isolated from the sea urchin hemicentrotus pulcherrimus collected from a tidal flat of naozhou island (20 degrees 52'n - 20 degrees 56'n 110 degrees 33'e - 110 degrees 38'e), leizhou bay, south china sea, china. | 2009 | 20112668 |
| gene diversity of cyp153a and alkb alkane hydroxylases in oil-degrading bacteria isolated from the atlantic ocean. | alkane hydroxylases, including the integral-membrane non-haem iron monooxygenase (alkb) and cytochrome p450 cyp153 family, are key enzymes in bacterial alkane oxidation. although both genes have been detected in a number of bacteria and environments, knowledge about the diversity of these genes in marine alkane-degrading bacteria is still limited, especially in pelagic areas. in this report, 177 bacterial isolates, comprising 43 genera, were obtained from 18 oil-degrading consortia enriched from ... | 2010 | 20148932 |
| [actinobacterial diversity of marine sediment samples from chile]. | the aim of this study was to investigate actinobacterial diversity in chilean marine sediments. | 2010 | 20815231 |
| [diversity of halophilic and halotolerant bacteria isolated from non-saline soil collected from xiaoxi national natural reserve, hunan province]. | to investigate the diversity of cultivable halophilic and halotolerant bacteria isolated from ordinary non-saline soil samples collected from xiaoxi national natural reserve (28 degrees 42' 15"-28 degrees 53' 15" n, 110 degrees 6' 50"-110 degrees 21' 35" e), hunan province, china. | 2010 | 21268889 |
| complete genome sequence of brachybacterium faecium type strain (schefferle 6-10). | brachybacterium faecium collins et al. 1988 is the type species of the genus, and is of phylogenetic interest because of its location in the dermabacteraceae, a rather isolated family within the actinobacterial suborder micrococcineae. b. faecium is known for its rod-coccus growth cycle and the ability to degrade uric acid. it grows aerobically or weakly anaerobically. the strain described in this report is a free-living, nonmotile, gram-positive bacterium, originally isolated from poultry deep ... | 2009 | 21304631 |
| brachybacterium squillarum sp. nov., isolated from salt-fermented seafood. | a gram-positive bacterium, strain m-6-3(t), was isolated from salt-fermented seafood in korea. the organism grew in 0-10 % (w/v) nacl and at 25-37 °c, with optimal growth occurring in 5 % nacl and at 28-30 °c. the peptidoglycan type was variation a4γ with meso-diaminopimelic acid as the diagnostic cell-wall diamino acid. the polar lipid profile of strain m-6-3(t) consisted of diphosphatidylglycerol, phosphatidylglycerol, an unidentified phospholipid and an unknown glycolipid. strain m-6-3(t) con ... | 2011 | 20543157 |
| removal of multi-heavy metals using biogenic manganese oxides generated by a deep-sea sedimentary bacterium - brachybacterium sp. strain mn32. | a deep-sea manganese-oxidizing bacterium, brachybacterium sp. strain mn32, showed high mn(ii) resistance (mic 55 mm) and mn(ii)-oxidizing/removing abilities. strain mn32 removed mn(ii) by two pathways: (1) oxidizing soluble mn(ii) to insoluble biogenic mn oxides - birnessite (delta-mno(2) group) and manganite (gamma-mnooh); (2) the biogenic mn oxides further adsorb more mn(ii) from the culture. the generated biogenic mn oxides surround the cell surfaces of strain mn32 and provide a high capacity ... | 2009 | 19383675 |
| devriesea agamarum gen. nov., sp. nov., a novel actinobacterium associated with dermatitis and septicaemia in agamid lizards. | five bacterial isolates were recovered from dermatitis or organ lesions of five agamid lizards. three strains were recovered from uromastyx species with dermatitis or septicaemia. all five isolates were gram-positive small rods that could not be identified using conventional phenotypic systems. they grew on sheep blood agar as small haemolytic colonies after 24 h of incubation at temperatures between 25 and 42 degrees c under aerobic, microaerophilic or anaerobic conditions. they were catalase-p ... | 2008 | 18768630 |
| brachybacterium phenoliresistens sp. nov., isolated from oil-contaminated coastal sand. | a coccoid- to ovoid-shaped, gram-positive, non-motile bacterial strain, designated phenol-at, was isolated from an oil-contaminated coastal sand sample collected from pingtung county, southern taiwan, and characterized by use of a polyphasic approach. phylogenetic analyses based on 16s rrna gene sequences showed that the novel strain formed a monophyletic branch at the periphery of the evolutionary radiation occupied by the genus brachybacterium in the family dermabacteraceae, class actinobacter ... | 2007 | 17978239 |
| brachybacterium zhongshanense sp. nov., a cellulose-decomposing bacterium from sediment along the qijiang river, zhongshan city, china. | a cellulose-decomposing bacterium, strain jbt, was isolated from sediments along the qijiang river, zhongshan city, china. results of morphological, biochemical and chemotaxonomic characterization and 16s rrna gene sequence analysis revealed that strain jbt belonged to the genus brachybacterium. insertion sequence-pcr fingerprinting patterns, dna base ratio analysis and dna-dna hybridization data showed that strain jbt differed from recognized species of the genus brachybacterium. based on polyp ... | 2007 | 17978212 |
| employment of 16 s rdna gene sequencing techniques to identify culturable environmental eubacteria in a tertiary referral hospital. | universal or 'broad-range' eubacterial polymerase chain reaction (pcr) was performed on 53 isolates from environmental water-associated sites in a haematology unit (n = 22) and the outer surfaces of cleaning lotion containers sited throughout a tertiary referral hospital (n = 31) 16 s rdna pcr was performed using two sets of universal primers, including the novel reverse primer, xb4, to generate a composite amplicon of 1068 bp, which was sequenced to obtain each isolate's identity. sequence anal ... | 2004 | 15142716 |
| brachybacterium muris sp. nov., isolated from the liver of a laboratory mouse strain. | a coccoid- to ovoid-shaped, gram-positive bacterial strain, designated c3h-21(t), was isolated from the liver of the laboratory mouse strain c3h/he and characterized by a polyphasic approach. the peptidoglycan type was variation a4gamma with meso-diaminopimelic acid as the diagnostic cell-wall diamino acid and an interpeptide bridge of d-asp-d-glu. the isolate contained menaquinone mk-7 (88 %) as the major component of the quinone system and minor amounts of menaquinone mk-8 (9 %) and menaquinon ... | 2003 | 14657129 |
| brachybacterium fresconis sp. nov. and brachybacterium sacelli sp. nov., isolated from deteriorated parts of a medieval wall painting of the chapel of castle herberstein (austria). | from two samples of microbial biofilms, damaging the mural paintings at the saint-catherine chapel of castle herberstein (austria), four and nine coryneform bacteria were isolated, respectively. a polyphasic taxonomic study of these isolates, including morphological, biochemical and chemotaxonomic characterization, rep-pcr fingerprinting, 16s rdna sequence analysis, dna base ratio and dna-dna hybridizations, allocated them to the genus brachybacterium. the isolates of the two samples both repres ... | 2002 | 12361268 |
| two coryneform bacteria isolated from the surface of french gruyère and beaufort cheeses are new species of the genus brachybacterium: brachybacterium alimentarium sp. nov. and brachybacterium tyrofermentans sp. nov. | new species names, brachybacterium alimentarium and brachybacterium tyrofermentans, are proposed for two coryneform bacteria isolated from the surfaces of gruyère and beaufort cheeses. these two species are similar in their biochemical and chemotaxonomic characteristics but distinct from previously described bacteria. the most distinctive characteristics are the presence of meso-diaminopimelic acid-containing peptidoglycan with a d-glu-d-asp interpeptide bridge and the presence of erythritol tei ... | 1996 | 8573524 |
| Genome sequence of Brachybacterium squillarum M-6-3(T), isolated from salt-fermented seafood. | Brachybacterium squillarum M-6-3(T) was isolated from salt-fermented seafood in Korea and belongs to the Dermabacteraceae, a rather isolated family within the actinobacterial suborder Micrococcineae. Here, we present the draft genome sequence of the type strain Brachybacterium squillarum M-6-3(T) (3,191,479 bp), a Gram-positive bacterium with high (72.8%) G+C content. | 2011 | 22038973 |
| brachybacterium saurashtrense sp. nov., a halotolerant root-associated bacterium with plant growth promoting potential. | a gram-positive, aerobic, non-motile, coccoid shaped, halotolerant bacterium (jg 06 t) was isolated from roots of salicornia brachiata, an extreme halophyte. phylogenetic analysis based on 16s rrna gene sequence showed sequence similarities of 99.2% to brachybacterium paraconglomeratum, 99.0% to b. conglomeratum, and 98.2% to b. faecium. dna-dna hybridization with the close relatives brachybacterium paraconglomeratum (dsm 46341t), b. conglomeratum (dsm 10241 t), b. faecium (dsm 4810t), b. tyrofe ... | 2011 | 21216918 |