| a tissue culture assay for direct detection of sodium channel blocking toxins in bacterial culture supernates. | a quantitative assay for sodium channel blocking toxins such as tetrodotoxin and saxitoxin has been developed for use with a microtitre plate reader. mouse neuroblastoma cells, which die rapidly in the presence of ouabain and veratridine, were protected by tetrodotoxin; surviving cells were detected by their uptake of the vital dye neutral red which was quantified with a microtitre plate reader at 540 nm. a sigmoidal dose response curve was obtained and tetrodotoxin concentrations were readily m ... | 1992 | 1320584 |
| structure of a colitose-containing o-specific polysaccharide of the marine bacterium pseudoalteromonas tetraodonis iam 14160(t). | o-specific polysaccharide was isolated by mild acid degradation of the lipopolysaccharide of pseudoalteromonas tetraodonis type strain iam 14160(t) and studied by sugar and methylation analyses along with 1h and 13c nmr spectroscopy, including 2d cosy, tocsy, 1h,(13)c hmqc and hmbc experiments. the polysaccharide was found to consist of hexasaccharide repeating units containing one residue each of d-gal, d-glca, d-galnac and d-glcnac and two residues of 3,6-dideoxy-l-xylo-hexose (colitose, col) ... | 2001 | 11423109 |
| the structure of gdp-4-keto-6-deoxy-d-mannose-3-dehydratase: a unique coenzyme b6-dependent enzyme. | l-colitose is a 3,6-dideoxysugar found in the o-antigens of some gram-negative bacteria such as escherichia coli and in marine bacteria such as pseudoalteromonas tetraodonis. the focus of this investigation, gdp-4-keto-6-deoxy-d-mannose-3-dehydratase, catalyzes the third step in colitose production, which is the removal of the hydroxyl group at c3' of gdp-4-keto-6-deoxymannose. it is an especially intriguing plp-dependent enzyme in that it acts as both a transaminase and a dehydratase. here we p ... | 2006 | 16943443 |
| identification of the pathogens associated with skin ulceration and peristome tumescence in cultured sea cucumbers apostichopus japonicus (selenka). | the aquaculture of sea cucumbers apostichopus japonicus (selenka) has developed rapidly in china in recent years, but is increasingly affected by diseases such as skin ulceration and peristome tumescence. previous studies on the pathogens causing these diseases focused largely on bacterial causes. in december 2008, we isolated four dominant bacterial species from lesions present in a. japonicus with the aforementioned diseases, from a farm in yangkou (qingdao, china). with two of these bacterial ... | 2010 | 20561977 |
| phenotypic and genetic characterization of bacteria isolated from diseased cultured sea cucumber apostichopus japonicus in northeastern china. | during the winter-spring from 2004 to 2006 in northeastern china cultured japanese sea cucumber apostichopus japonicus suffered from a serious disease. clinical signs included swollen mouth, skin ulceration and massive mortality. clinical samples taken during this period were studied. thirty-one bacterial samples were isolated from diseased sea cucumbers and identified through biochemical tests, 16s rrna gene sequence analysis and pcr amplification, followed by pathogenicity determination. the r ... | 2010 | 21133322 |
| [enrichment and diversity analyis of arsenite-resistant bacteria in deep sea sediment samples from southwest indian ocean ridge]. | the aim of this study was to assess the diversity of arsenite-resistant bacteria in deep sea sediment of the southwest indian ocean ridge. | 2008 | 19160816 |
| retrieval of the species alteromonas tetraodonis simidu et al. 1990 as pseudoalteromonas tetraodonis comb. nov. and emendation of description. | a polyphasic taxonomy study was undertaken of three strains of pseudoalteromonas haloplanktis subsp. tetraodonis (simidu et al. 1990) gauthier et al. 1995. dna was prepared from each of the strains and genomic relatedness was measured by dna-dna hybridization. strains kmm 458t and iam 14160t shared 99% genetic relatedness, but were only 48-49% related to the type strain of pseudoalteromonas haloplanktis subsp. haloplanktis, iam 12915t. the third strain, p. haloplanktis subsp. tetraodonis a-m, sh ... | 2001 | 11411675 |
| phenotypic diversity of pseudoalteromonas citrea from different marine habitats and emendation of the description. | four strains of marine, aerobic, agar-decomposing bacteria with one polar flagellum and with dna g + c contents of 38.9-40.2 mol% were isolated from the far-eastern mussels crenomytilus grayanus and patinopecten yessoensis. these four strains were identified as pseudoalteromonas; however, they were phenotypically different from species described previously according to carbon compound utilization tests and the biolog identification system. high agar-decomposing activity was found in two strains, ... | 1998 | 9542094 |
| effect of phosphate concentration on production of tetrodotoxin by alteromonas tetraodonis. | tetrodotoxin production by alteromonas tetraodonis occurred during the stationary phase of growth and was regulated by phosphate concentration; toxin production was repressed if phosphate was added at the onset of stationary phase and was over 100-fold greater in phosphate-limited cultures than in cultures in which phosphate was not limiting. | 1993 | 8285702 |
| phylogenetic analysis of the genera alteromonas, shewanella, and moritella using genes coding for small-subunit rrna sequences and division of the genus alteromonas into two genera, alteromonas (emended) and pseudoalteromonas gen. nov., and proposal of twelve new species combinations. | small-subunit ribosomal dna sequences were determined for 17 strains belonging to the genera alteromonas, shewanella, vibrio, and pseudomonas, and these sequences were analyzed by phylogenetic methods. the resulting data confirmed the existence of the genera shewanella and moritella, but suggested that the genus alteromonas should be split into two genera. we propose that a new genus, the genus pseudoalteromonas, should be created to accommodate 11 species that were previously alteromonas specie ... | 1995 | 7547295 |
| taxonomy of four marine bacterial strains that produce tetrodotoxin. | four strains of tetrodotoxin-producing bacteria isolated from a red alga and from pufferfish were characterized. two of these strains are members of the genus listonella macdonell and colwell. the phenotypic characteristics, guanine-plus-cytosine contents, and base sequences of the 16s rrnas of these organisms indicated that they are members of listonella pelagia (vibrio pelagius) biovar ii. the other two strains are members of the genus alteromonas baumann et al. and the genus shewanella macdon ... | 1990 | 2275851 |
| genetic and biochemical characterization of the pseudoalteromonas tetraodonis alkaline κ-carrageenase. | an alkaline κ-carrageenase, cgk-k142, was found in the culture broth of a deep-sea bacterium, pseudoalteromonas tetraodonis jam-k142. a gene for the enzyme was cloned and expressed. purified recombinant cgk-k142 (rcgk-k142) showed an optimal ph of about 8.8 in glycine-naoh buffer at 30 °c and of about 8.0 in mops buffer at 50 °c. the optimal temperature for the enzyme was 55 °c at ph 8.0. rcgk-k142 was unstable, but λ- and ι-carrageenans, non-degradative substrate homologs, extensively enhanced ... | 2012 | 22451392 |
| draft genome sequence of pseudoalteromonas tetraodonis strain ucd-sed8 (phylum gammaproteobacteria). | here, we present the draft genome sequence of pseudoalteromonas tetraodonis ucd-sed8, a marine bacterium normally associated with the production of tetrodotoxin in pufferfish. this strain was isolated from sediment samples surrounding zostera marina roots collected from bodega marine, california. the assembly consists of 4,017,727 bp contained in 35 contigs. | 2015 | 26543114 |
| draft genome sequence of pseudoalteromonas tetraodonis strain mqs005, a bacterium with potential quorum-sensing regulation. | we present here the draft genome sequence of pseudoalteromonas tetraodonis strain mqs005, a bacterium possessing potential quorum-sensing regulatory activity. this strain was isolated from water from the south china sea, people's republic of china. the assembly consists of 4,252,538 bp and contains 144 contigs, with a g+c content of 41.85%. | 2016 | 27491986 |
| pseudoalteromonas aestuariivivens sp. nov., isolated from a tidal flat. | a gram-stain-negative, aerobic, motile, rod-shaped or ovoid bacterial strain, designated db-2t, was isolated from a tidal flat of the yellow sea in south korea, and subjected to a taxonomic study using a polyphasic approach. strain db-2t grew optimally at 30 °c, at ph 7.0-8.0 and in the presence of 2.0-3.0 % (w/v) nacl. phylogenetic trees based on 16s rrna gene sequences showed that strain db-2t belonged to the genus pseudoalteromonas. strain db-2t exhibited 16s rrna gene sequence similarities o ... | 2016 | 26921230 |