| increased protein content of chickpea (cicer arietinum l.) inoculated with arbuscular mycorrhizal fungi and nitrogen-fixing bacteria under water deficit conditions. | chickpea (cicer arietinum l.) is a widely cropped pulse and an important source of proteins for humans. in mediterranean regions it is predicted that drought will reduce soil moisture and become a major issue in agricultural practice. nitrogen (n)-fixing bacteria and arbuscular mycorrhizal (am) fungi have the potential to improve plant growth and drought tolerance. the aim of the study was to assess the effects of n-fixing bacteria and am fungi on the growth, grain yield and protein content of c ... | 2017 | 28071807 |
| survey of chickpea rhizobia diversity in portugal reveals the predominance of species distinct from mesorhizobium ciceri and mesorhizobium mediterraneum. | several mesorhizobium species are able to induce effective nodules in chickpea, one of the most important legumes worldwide. our aims were to examine the biogeography of chickpea rhizobia, to search for a predominant species, and to identify the most efficient microsymbiont, considering portugal as a case study. one hundred and ten isolates were obtained from continental portugal and madeira island. the 16s ribosomal rna gene phylogeny revealed that isolates are highly diverse, grouping with mos ... | 2009 | 19468700 |
| mesorhizobium metallidurans sp. nov., a metal-resistant symbiont of anthyllis vulneraria growing on metallicolous soil in languedoc, france. | a polyphasic taxonomic approach was used to characterize 31 rhizobial isolates obtained from anthyllis vulneraria, a metallicolous legume species, growing close to a zinc mine in the south of france (saint laurent le minier). comparative analysis of nearly full-length 16s rrna gene sequences showed that these gram-negative bacteria belonged to the genus mesorhizobium and that they were related most closely to mesorhizobium tianshanense ors 2640(t). the phylogenetic relationships of these isolate ... | 2009 | 19329619 |
| mesorhizobium caraganae sp. nov., a novel rhizobial species nodulated with caragana spp. in china. | five rhizobial strains representing the largest group in the genus mesorhizobium associated with caragana spp. in china were characterized taxonomically. phylogenetic analysis based on 16s rrna gene sequences indicated that these microsymbionts belonged to the genus mesorhizobium, with mesorhizobium tianshanense usda 3592(t), mesorhizobium temperatum sdw018(t) and mesorhizobium mediterraneum upm-ca36(t) as the closest neighbours (>/=99.5 % 16s rrna gene sequence similarity). genotypic fingerprin ... | 2008 | 18984708 |
| strains of mesorhizobium amorphae and mesorhizobium tianshanense, carrying symbiotic genes of common chickpea endosymbiotic species, constitute a novel biovar (ciceri) capable of nodulating cicer arietinum. | to identify several strains of mesorhizobium amorphae and mesorhizobium tianshanense nodulating cicer arietinum in spain and portugal, and to study the symbiotic genes carried by these strains. | 2007 | 17397480 |
| rhizobial strain involvement in plant growth, nodule protein composition and antioxidant enzyme activities of chickpea-rhizobia symbioses: modulation by salt stress. | mesorhizobium ciceri, mesorhizobium mediterraneum and sinorhizobium medicae strains showed different symbiotic performances when inoculated to chickpea (cicer arietinum l., cv. chetoui) at unstressed conditions and under salt stress. the analysis of nodular proteic composition and antioxidant enzyme activities revealed a polymorphism of patterns on sds and native page suggesting a potential dependence on the bacterial partner. salt effect was analysed on plant growth, nitrogen fixation and antio ... | 2004 | 15474377 |
| genotypic alteration and competitive nodulation of mesorhizobium muleiense against exotic chickpea rhizobia in alkaline soils. | mesorhizobium muleiense, mesorhizobium mediterraneum and mesorhizobium ciceri are chickpea (cicer arietinum l.) rhizobia that share a high similarity of the symbiotic genes nodc and nifh, but they have different geographic distributions. m. muleiense has been isolated and found only in alkaline soils of xinjiang, china, whereas the other two strains have been found in the mediterranean and india. to investigate the species stability of m. muleiense during natural evolution and its capability of ... | 2014 | 25123757 |
| genomic heterogeneity of strains nodulating chickpeas (cicer arietinum l.) and description of rhizobium mediterraneum sp. nov. | the genetic diversity of chickpea strains was studied by using 30 isolates obtained from nodules on chickpeas growing in uninoculated fields over a wide geographic range. the following taxonomic approaches were used: dna-dna relatedness analysis, restriction fragment length polymorphism analysis of the amplified 16s ribosomal dna (rdna) intergenic spacer (igs), and total 16s rrna sequence analysis. the division of chickpea-infective strains into two major phylogenetic groups (groups a and b) tha ... | 1995 | 7547282 |
| analysis of cellular fatty acids and phenotypic relationships of agrobacterium, bradyrhizobium, mesorhizobium, rhizobium and sinorhizobium species using the sherlock microbial identification system. | previous studies have demonstrated that cellular fatty acid analysis is a useful tool for identifying unknown strains of rhizobia and establishing taxonomic relationships between the species. in this study, the fatty acid profiles of over 600 strains belonging to the genera agrobacterium, bradyrhizobium, mesorhizobium, rhizobium and sinorhizobium were evaluated using the gaschromatography-based sherlock microbial identification system (mis). data collected with the mis showed that the three phyl ... | 2000 | 10758890 |
| phenotypic and molecular characterization of chickpea rhizobia isolated from different areas of tunisia. | several phenotypic markers were used in this study to determine the biodiversity of rhizobial strains nodulating cicer arietinum l. in various areas of tunisia. they include symbiotic traits, the use of 21 biochemical substrates, and tolerance to salinity and ph. in addition, restriction fragment length polymorphisms (rflps) of pcr-amplified 16s rdna were compared with those of reference strains. numeric analysis of the phenotypic characteristics showed that the 48 strains studied fell into thre ... | 2007 | 17538653 |
| invasive robinia pseudoacacia in china is nodulated by mesorhizobium and sinorhizobium species that share similar nodulation genes with native american symbionts. | the aim of this work is to describe the diversity of potentially symbiotic bacteria associated with the invasive introduced legume robinia pseudoacacia in china. thirty-three isolates from 33 separate trees and nodules were characterized using restriction length fragment polymorphism and sequencing of 16s rrna, noda, nodc and nifh genes. their 16s rrna gene patterns and sequences placed them in three clades: 85% of isolates were related to the mesorhizobium mediterraneum/temperatum group, wherea ... | 2009 | 19416352 |
| mesorhizobium robiniae sp. nov., isolated from root nodules of robinia pseudoacacia. | previously, five rhizobial strains isolated from root nodules of robinia pseudoacacia were assigned to the same genospecies on the basis of identical 16s rrna gene sequences and phylogenetic analyses of the noda, nodc and nifh genes, in which the five isolates formed a well-supported group that excluded other sequences found in public databases. in this study, the 16s rrna gene sequence similarities between the isolates and mesorhizobium mediterraneum upm-ca36(t) and mesorhizobium temperatum sdw ... | 2010 | 20008111 |
| aurantimonas coralicida gen. nov., sp. nov., the causative agent of white plague type ii on caribbean scleractinian corals. | a bacterium previously isolated from a diseased colony of the scleractinian coral dichocoenia stokesi (common name elliptical star coral) was subjected to a detailed polyphasic taxonomic characterization. the isolate, designated wp1t, was halophilic and strictly aerobic and formed golden-orange-pigmented colonies after prolonged incubation. cells of wp1t were gram-negative, rod-shaped and showed a characteristic branching rod morphology. chemotaxonomically, wp1t was characterized by having q-10 ... | 2003 | 12892136 |
| genetic diversity and host range of rhizobia nodulating lotus tenuis in typical soils of the salado river basin (argentina). | a total of 103 root nodule isolates were used to estimate the diversity of bacteria nodulating lotus tenuis in typical soils of the salado river basin. a high level of genetic diversity was revealed by repetitive extragenic palindromic pcr, and 77 isolates with unique genomic fingerprints were further differentiated into two clusters, clusters a and b, after 16s rrna restriction fragment length polymorphism analysis. cluster a strains appeared to be related to the genus mesorhizobium, whereas cl ... | 2009 | 19074602 |
| different mesorhizobium species associated with caragana carry similar symbiotic genes and have common host ranges. | fourteen strains representing 11 caragana-nodulating mesorhizobium genomic species were identified as representing mesorhizobium amorphae, mesorhizobium huakuii, mesorhizobium septentrionale and groups related to mesorhizobium plurifarium, mesorhizobium temperatum, mesorhizobium tianshanense and mesorhizobium mediterraneum by sequencing of the 16s rrna gene, 16s-23s internal transcribed spacer, partial housekeeping reca gene, and previously performed sodium dodecyl sulfate polyacrylamide gel ele ... | 2008 | 18422620 |
| the symbiotic performance of chickpea rhizobia can be improved by additional copies of the clpb chaperone gene. | the clpb chaperone is known to be involved in bacterial stress response. moreover, recent studies suggest that this protein has also a role in the chickpea-rhizobia symbiosis. in order to improve both stress tolerance and symbiotic performance of a chickpea microsymbiont, the mesorhizobium mediterraneum upm-ca36t strain was genetically transformed with pphu231 containing an extra-copy of the clpb gene. to investigate if the clpb-transformed strain displays an improved stress tolerance, bacterial ... | 2016 | 26845770 |