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adaptation and evaluation of the bottle assay for monitoring insecticide resistance in disease vector mosquitoes in the peruvian amazon.the purpose of this study was to establish whether the "bottle assay", a tool for monitoring insecticide resistance in mosquitoes, can complement and augment the capabilities of the established who assay, particularly in resource-poor, logistically challenging environments.200919728871
intraespecific variation on the aedeagus of anopheles oswaldoi (peryassú) (diptera: culicidae).anopheles (nyssorhynchus) oswaldoi (peryassú) comprises a species complex in south america. specimens from two localities in east mata atlântica were characterized both at the morphological and molecular level as an. oswaldoi s.s. intraspecific variation of the shape of the apex of the aedeagus of the male genitalia of specimens of anopheles (nyssorhynchus) oswaldoi s.s. from vale do ribeira, mata atlântica, são paulo state, brazil, was observed. distinctive aedeagus of the specimens from vale d ...200919347109
morphological analysis of three populations of anopheles (nyssorhynchus) nuneztovari gabaldón (diptera: culicidae) from colombia.based on the results of comparative analyses of 1,039 specimens of several progenies of anopheles nuneztovarifrom three localities in colombia, eight costal wing spot patterns were observed. patterns i and iii were the most frequent: 77.96% and 11.36%, respectively. using the diagnostic characters ratio of the length of the basal dark area of hind tarsomere ii/length of hind tarsomere ii, ratio of the length of the humeral pale spot/length of the pre-humeral dark spot, and the ratio of the lengt ...200818368239
spatial distribution and habitat characterisation of anopheles larvae along the kenyan coast.a study was conducted to characterise larval habitats and to determine spatial heterogeneity of the anopheles mosquito larvae. the study was conducted from may to june 1999 in nine villages along the kenyan coast.200717378216
molecular identification of palearctic members of anopheles maculipennis in northern iran.members of anopheles maculipennis complex are effective malaria vectors in europe and the caspian sea region in northern iran, where malaria has been re-introduced since 1994. the current study has been designed in order to provide further evidence on the status of species composition and to identify more accurately the members of the maculipennis complex in northern iran.200717233887
evidence for temporal population replacement and the signature of ecological adaptation in a major neotropical malaria vector in amazonian peru.the major neotropical malaria vector, anopheles darlingi, was reintroduced into the iquitos, loreto, peru area during the early 1990s, where it displaced other anophelines and caused a major malaria epidemic. since then, case numbers in loreto have fluctuated, but annual increases have been reported since 2012.201526415942
new classification of natural breeding habitats for neotropical anophelines in the yanomami indian reserve, amazon region, brazil and a new larval sampling methodology.here we present the first in a series of articles about the ecology of immature stages of anophelines in the brazilian yanomami area. we propose a new larval habitat classification and a new larval sampling methodology. we also report some preliminary results illustrating the applicability of the methodology based on data collected in the brazilian amazon rainforest in a longitudinal study of two remote yanomami communities, parafuri and toototobi. in these areas, we mapped and classified 112 na ...026517655
an overview of malaria transmission from the perspective of amazon anopheles vectors.in the americas, areas with a high risk of malaria transmission are mainly located in the amazon forest, which extends across nine countries. one keystone step to understanding the plasmodium life cycle in anopheles species from the amazon region is to obtain experimentally infected mosquito vectors. several attempts to colonise anopheles species have been conducted, but with only short-lived success or no success at all. in this review, we review the literature on malaria transmission from the ...201525742262
molecular differentiation in natural populations of anopheles oswaldoi sensu lato (diptera: culicidae) from the brazilian amazon, using sequences of the coi gene from mitochondrial dna.anopheles (nyssorhynchus) oswaldoi (peryassú, 1922) s. l., which has been incriminated as a potential human malaria vector in western brazilian amazon, may constitute a cryptic species complex. however, the most recent study with isozymes indicated high similarity among samples from the states of acre, amazonas and rondônia in the brazilian amazon. in the present study, 45 individuals were sequenced from sena madureira (state of acre), coari (state of amazonas), são miguel (state of rondônia), a ...200617117365
comparison of transmission parameters between anopheles argyritarsis and anopheles pseudopunctipennis in two ecologically different localities of bolivia.anopheles (anopheles) pseudopunctipennis is a recognized malaria vector in the slopes of the andes of bolivia. there, other species might be involved in malaria transmission and one candidate could be anopheles argyritarsis. although it is generally admitted that this species is not a malaria vector in the neotropical region, its potential role in transmission is still controversial and this situation has to be cleared, at least for bolivia. comparing the vectorial efficiency of an. pseudopuncti ...201323941216
evidence of new species for malaria vector anopheles nuneztovari sensu lato in the brazilian amazon region.anopheles nuneztovari sensu lato comprises cryptic species in northern south america, and the brazilian populations encompass distinct genetic lineages within the brazilian amazon region. this study investigated, based on two molecular markers, whether these lineages might actually deserve species status.201627068120
experimental infection of the neotropical malaria vector anopheles darlingi by human patient-derived plasmodium vivax in the peruvian amazon.malaria transmission from humans to mosquitoes is modulated by human host immune factors. understanding mechanisms by which the human host response may impair parasite infectivity for mosquitoes has direct implications for the development of transmission-blocking vaccines. we hypothesized that despite a low transmission intensity of malaria in the peruvian amazon region of iquitos, transmission-blocking immunity against plasmodium vivax might be common, given an unexpectedly high proportion of a ...200617038681
tetrahymenidae infection in mosquito populations in a malaria-endemic region of the amazon.in a field survey performed in a malaria-endemic region of northern amazon, brazil, we encountered ciliate protozoa of the family tetrahymenidae infecting adults and larvae of the following mosquito species: culex sp., anopheles albitarsis l.s., anopheles strodei, anopheles mattogrossensis, anopheles darlingi, and anopheles oswaldoi l.s. based on morphological features and life style, we have tentatively identified the parasite as lambornella sp. the association appears pathogenic for the mosqui ...200616497321
isozyme similarity in anopheles oswaldoi sensu lato (diptera: culicidae) from the amazon region, brazil.isozyme electrophoresis studies were conduced on anopheles oswaldoi sensu lato from three states in amazonian (brazil), including acre, where this taxon has been incriminated as a potential human malaria vector. the 13 enzymes analyzed yielded a total of 20 loci. of these, 10 were monomorphic in the three samples. diagnostic loci were not found. the measures of genetic variability showed mean number of alleles per locus between 1.5 and 2.0, percentage of polymorphic loci of 40%, and mean heteroz ...200515962781
contribution of fish farming ponds to the production of immature anopheles spp. in a malaria-endemic amazonian town.in the past decade fish farming has become an important economic activity in the occidental brazilian amazon, where the number of new fish farms is rapidly increasing. one of the primary concerns with this phenomenon is the contribution of fishponds to the maintenance and increase of the anopheline mosquito population, and the subsequent increase in human malaria burden. this study reports the results of a 2-year anopheline abundance survey in fishponds and natural water bodies in a malaria-ende ...201526573145
is there an efficient trap or collection method for sampling anopheles darlingi and other malaria vectors that can describe the essential parameters affecting transmission dynamics as effectively as human landing catches? - a review.distribution, abundance, feeding behaviour, host preference, parity status and human-biting and infection rates are among the medical entomological parameters evaluated when determining the vector capacity of mosquito species. to evaluate these parameters, mosquitoes must be collected using an appropriate method. malaria is primarily transmitted by anthropophilic and synanthropic anophelines. thus, collection methods must result in the identification of the anthropophilic species and efficiently ...025185008
[egg morphology as an indirect method to identify anopheles benarrochi, anopheles oswaldoi and anopheles rangeli (diptera: culicidae)].in the department of putumayo in southern colombia, malaria transmission has continued in the absence of the 4 traditional latin american vector species--anopheles darlingi, anopheles nuneztovari, anopheles albimanus or anopheles trinkae. human bait collections yielded anopheles mosquitoes and a morphological variant of anopheles benarrochi, the adult females of which can easily be misidentified as anopheles oswaldoi. species identification of females of anopheles in the subgenus nyssorhynchus i ...200314968916
factors associated with distribution of anopheles aquasalis and anopheles oswaldoi (diptera: culicidae) in a malarious area, northeastern venezuela.spatial and temporal abundance patterns of anopheline larvae and their relationships with wetland conditions were studied in an endemic malaria area in northeastern venezuela, where anopheles aquasalis curry is the main vector. larvae were sampled over a 2-yr period in 7 wetland types (brackish and freshwater herbaceous swamps, mangrove swamps, freshwater ponds, clear-cut marsh forests, small irrigation canals, and swamp forests), covering 3 environmental gradients (salinity, aquatic vegetation, ...200010730493
comparative susceptibility of two members of the anopheles oswaldoi complex, an. oswaldoi and an. konderi, to infection by plasmodium vivax.we compared the susceptibility of anopheles oswaldoi and an. konderi to infection by plasmodium vivax based on the proportion of mosquitoes presenting oocysts and sporozoites. anophelines were captured in the state of acre and rondônia, brazilian amazon, and used to obtain f1 progenies. after emergence of adults, male genitalia of mosquitoes of each family were dissected in order to identify them as either an. oswaldoi or an. konderi. f1 progenies of field-captured an. oswaldoi, an. konderi and ...199910674081
malaria entomological risk factors in relation to land cover in the lower caura river basin, venezuela.to explore the effects of deforestation and resulting differences in vegetation and land cover on entomological parameters, such as anopheline species composition, abundance, biting rate, parity and entomological inoculation rate (eir), three villages were selected in the lower caura river basin, state of bolívar, venezuela. all-night mosquito collections were conducted between march 2008-january 2009 using cdc light traps and mosquito magnet® liberty plus. human landing catches were performed b ...023579803
novel strategies lead to pre-elimination of malaria in previously high-risk areas in suriname, south america.suriname was a high malaria risk country before the introduction of a new five-year malaria control program in 2005, the medical mission malaria programme (mm-mp). malaria was endemic in the forested interior, where especially the stabile village communities were affected.201222230221
molecular taxonomy of anopheles (nyssorhynchus) benarrochi (diptera: culicidae) and malaria epidemiology in southern amazonian peru.anopheline specimens were collected in 2011 by human landing catch, shannon and cdc traps from the malaria endemic localities of santa rosa and san pedro in madre de dios department, peru. most specimens were either anopheles (nyssorhynchus) benarrochi b or an. (nys.) rangeli, confirmed by polymerase chain reaction-restriction fragment length polymorphism-internal transcribed spacer 2 (pcr-rflp-its2) and, for selected individuals, its2 sequences. a few specimens from lupuna, loreto department, n ...201223243107
larval habitats of anopheline mosquitoes in the upper orinoco, venezuela.survey of larval habitats of anopheline mosquitoes was conducted in ocamo in the state of amazonas, southern venezuela. the sampled habitats belonged to three different hydrological types: lagoons (26 habitats), forest pools including flooded forest (16 habitats), and forest streams (4 habitats). out of 46 habitats surveyed, 31 contained anopheline larvae. six species were found: anopheles darlingi, anopheles triannulatus, anopheles oswaldoi, anopheles peryassui, anopheles punctimacula, and anop ...199910672542
correlation between positive serology for plasmodium vivax-like/plasmodium simiovale malaria parasites in the human and anopheline populations in the state of acre, brazil.antibodies against the plasmodium vivax-like/p. simiovale malaria parasite circumsporozoite repeat peptide (apganqeggaa)3 were determined by enzyme-linked immunosorbent assay (elisa) in 120 sera randomly collected in 1994 from adults in 3 localities of the malaria endemic area in the state of acre, brazil; antibody was detected in 18 (15%). a 'sandwich' elisa using monoclonal antibody (mab) pam 172, directed against the same peptide, was carried out on 1207 anopheles oswaldoi, 12 of which (1.0%) ...19989764317
comparative egg morphology of six species of the albimanus section of anopheles (nyssorhynchus) (diptera:culicidae).scanning electron micrographs were used to describe and compare structures of eggs obtained from wild-caught females of 6 species of the albimanus section of anopheles (nyssorhynchus) from south america, which includes important regional vectors of human malaria. among species from the oswaldoi complex, eggs of anopheles oswaldoi (peryassu) were not differentiated from those of its sibling anopheles konderi galvão & damasceno, and eggs of the former species from brazil, ecuador, and suriname sho ...19979103756
diel oviposition and fecundity of anopheles oswaldoi in trinidad, west indies.the diel oviposition periodicity of wild-caught anopheles oswaldoi collected from the forest-ecotone habitat in valencia, trinidad, was studied in the laboratory by recording the performance of egg-laying individuals and a colony at 2-h intervals. oviposition was almost exclusively nocturnal, with 95.3% of eggs and 91.3% of oviposition occurrences being observed during the scotophase. during the rest of the day, only 4.7% of eggs (8.7% occurrences) were recorded after dawn (0600-0800 h). wild-ca ...19968887217
anopheles oswaldoi a potential malaria vector in acre, brazil. 19968758058
anophelines in the state of acre, brazil, infected with plasmodium falciparum, p. vivax, the variant p. vivax vk247 and p. malariae.anophelines collected indoors and in the peri-domiciliary area in 3 localities in the amazon region, state of acre, brazil, from august 1990 to january 1991 were examined by enzyme-linked immunosorbent assay (elisa) using specific monoclonal antibodies directed against the repeats of the circumsporozoite proteins of plasmodium falciparum, p. vivax, p. vivax v247, and p. malariae. of the 3056 specimens collected, 2610 were anopheles oswaldoi, 362 a. deaneorum, 60 a. triannulatus and 24 were a. da ...19938249059
effectiveness of mosquito magnet® trap in rural areas in the southeastern tropical atlantic forest.traps are widely employed for sampling and monitoring mosquito populations for surveillance, ecological and fauna studies. considering the importance of assessing other technologies for sampling mosquitoes, we addressed the effectiveness of mosquito magnet® independence (mmi) in comparison with those of the cdc trap with co2 and lurex3® (cdc-a) and the cdc light trap (cdc-lt). field collections were performed in a rural area within the atlantic forest biome, southeastern state of são paulo, braz ...201425424445
improved molecular technique for the differentiation of neotropical anopheline species.we evaluated a pcr-rflp of the ribosomal internal transcribed spacer 2 region (its2) to distinguish species of anopheles commonly reported in the amazon and validated this method using reared f1 offspring. the following species of anopheles were used for molecular analysis: an. (nys.) benarrochi, an. (nys.) darlingi, an. (nys.) nuneztovari, an. (nys.) konderi, an. (nys.) rangeli, and an. (nys.) triannulatus sensu lato (s.l.). in addition, three species of the subgenus anopheles, an. (ano.) forat ...200818337348
Amazonian malaria: Asymptomatic human reservoirs, diagnostic challenges, environmentally driven changes in mosquito vector populations, and the mandate for sustainable control strategies.Across the Americas and the Caribbean, nearly 561,000 slide-confirmed malaria infections were reported officially in 2008. The nine Amazonian countries accounted for 89% of these infections; Brazil and Peru alone contributed 56% and 7% of them, respectively. Local populations of the relatively neglected parasite Plasmodium vivax, which currently accounts for 77% of the regional malaria burden, are extremely diverse genetically and geographically structured. At a time when malaria elimination is ...201122015425
comparison of capture methods for the diagnosis of adult anopheline populations from state of mato grosso, brazil.the present study compares human landing catches of primary malaria vectors with two alternative methods of capture: the shannon trap and the mosquito magnet.201121860993
the influence of mosquito resting behaviour and associated microclimate for malaria risk.abstract:201121736735
the dominant anopheles vectors of human malaria in the americas: occurrence data, distribution maps and bionomic précis.abstract:201020712879
species composition and natural infectivity of anthropophilic anopheles (diptera: culicidae) in the states of córdoba and antioquia, northwestern colombia.malaria is a serious health problem in the states of córdoba and antioquia, northwestern colombia, where 64.4% of total colombian cases were reported in 2007. because little entomological information is available in this region, the aim of this work was to identify the anopheles species composition and natural infectivity of mosquitoes distributed in seven localities with highest malaria transmission. a total of 1,768 anopheles mosquitoes were collected using human landing catches from march 200 ...200920140372
abundance, behavior and entomological inoculation rates of anthropophilic anophelines from a primary colombian malaria endemic area.in colombia for several years, the urabá-bajo cauca and alto sinú region has registered the highest numbers of malaria cases in the country. malaria vector incrimination and the characterization of entomological parameters will allow for a better understanding of malaria transmission dynamics and the design of effective vector control strategies for this region.201323497535
insight into anopheles (nyssorhynchus) (diptera: culicidae) species from brazil.anopheles (nyssorhynchus) benarrochi s.l., anopheles (nyssorhynchus) oswaldoi s.l., and anopheles (nyssorhynchus) konderi s.l. collected in acrelandia, state of acre, brazil, were identified based on morphological characters of the male genitalia, fourth-instar larvae, and pupae. morphological variation was observed in the male genitalia of these species in comparison with specimens from other localities in brazil. dna sequence from the nuclear ribosomal second internal transcribed spacer of ind ...200819058619
molecular comparison of topotypic specimens confirms anopheles (nyssorhynchus) dunhami causey (diptera: culicidae) in the colombian amazon.the presence of anopheles (nyssorhynchus) dunhami causey in colombia (department of amazonas) is confirmed for the first time through direct comparison of mtdna cytochrome c oxidase i (coi) barcodes and nuclear rdna second internal transcribed spacer (its2) sequences with topotypic specimens of an. dunhami from tefé, brazil. an. dunhami was identified through retrospective correlation of dna sequences following misidentification as anopheles nuneztovari s.l. using available morphological keys fo ...021120360
hyperendemic malaria transmission in areas of occupation-related travel in the peruvian amazon.plasmodium vivax and plasmodium falciparum cause a significant illness burden in peru. anopheline indices for populated communities in the peri-iquitos region of loreto have been reported to be remarkably low, with entomological inoculation rates (eir) estimated at one to 30 infective bites per year based on a few studies in close proximity to the urban centre of iquitos and surrounding deforested areas. local reports suggest that a large number of the reported cases are contracted outside of po ...201323724869
integrated vector management targeting anopheles darlingi populations decreases malaria incidence in an unstable transmission area, in the rural brazilian amazon.studies on vector behaviour should be conducted in order to evaluate the effectiveness of vector control measures on malaria protection in endemic areas of latin america, where p. vivax predominates. this work aims to investigate the fauna of anopheline mosquitoes and verify the impact of integrated vector management in two colonization projects in the careiro municipality, western brazilian amazon.201223088224
new highland distribution records of multiple anopheles species in the ecuadorian andes.several recent climate change reviews have stressed the possibility of some malaria vectors occupying regions of higher altitudes than previously recorded. indeed, highland malaria has been observed in several african nations, possibly attributable to changes in land use, vector control and local climate. this study attempts to expand the current knowledge of the distribution of common anopheles species in ecuador, with particular attention to highland regions (> 500 m) of the andes.201121835004
mosquito magnet® liberty plus trap baited with octenol confirmed best candidate for anopheles surveillance and proved promising in predicting risk of malaria transmission in french guiana.in french guiana, mosquito magnet® liberty plus trap baited with octenol (mmoct) has been proposed for sampling anopheles darlingi after comparison with cdc light trap and human landing catch (hlc). however, other available lures were not tested. the current study compared mmoct and mm baited with lurex™ (mmlur) to hlc, and analysed entomological data from mmoct collection with malaria cases to facilitate malaria surveillance.201425260354
natural plasmodium infection in monkeys in the state of rondônia (brazilian western amazon).simian malaria is still an open question concerning the species of plasmodium parasites and species of new world monkeys susceptible to the parasites. in addition, the lingering question as to whether these animals are reservoirs for human malaria might become important especially in a scenario of eradication of the disease. to aid in the answers to these questions, monkeys were surveyed for malaria parasite natural infection in the amazonian state of rondônia, brazil, a state with intense envir ...201323731624
malaria knowledge, concern, land management, and protection practices among land owners and/or managers in lowland versus highland ecuador.to control malaria effectively, it is essential to understand the current knowledge, beliefs, concerns, land management practices, and mosquito bite protection methods in use by citizens. this study presents a comparative, quantitative, interview-based study of land owners and/or managers (n = 262) in the ecuadorian lowlands (presently considered malarious) (n = 131) and highlands (potentially malarious in the future) (n = 131). although respondents had a strong understanding of where the diseas ...201222363897
implications for changes in anopheles darlingi biting behaviour in three communities in the peri-iquitos region of amazonian peru.malaria transmission in the peri-iquitos region of amazonian peru has been designated as seasonal and hypo-endemic with recently described hyper-endemic hotspots. despite relatively recent distribution of long-lasting insecticidal bed nets (llins), malaria in amazonian peru persists and increased substantially in 2014 compared to previous years. anopheles darlingi, identified as the main malaria vector, is known for its variable behaviour depending on locality and environment.201526223450
preliminary biological studies on larvae and adult anopheles mosquitoes (diptera: culicidae) in miraflores, a malaria endemic locality in guaviare department, amazonian colombia.in the malaria endemic municipality of miraflores in southeastern amazonian colombia, several aspects of the biology of local anopheles species were investigated to supplement the limited entomological surveillance information available and to provide baseline data for malaria prevention and vector control. anopheles darlingi root, 1926 was the most abundant species (95.6%), followed by anopheles braziliensis (chagas) (3.6%) and anopheles oswaldoi s.l. (peryassu) (0.7%). during the dry season, e ...025276930
malaria on the guiana shield: a review of the situation in french guiana.in a climate of growing concern that plasmodium falciparum may be developing a drug resistance to artemisinin derivatives in the guiana shield, this review details our current knowledge of malaria and control strategy in one part of the shield, french guiana. local epidemiology, test-treat-track strategy, the state of parasite drug resistance and vector control measures are summarised. current issues in terms of mobile populations and legislative limitations are also discussed.201425184998
molecular investigation into a malaria outbreak in cusco, peru: plasmodium falciparum bv1 lineage is linked to a second outbreak in recent times.in november 2013, a plasmodium falciparum malaria outbreak of 11 cases occurred in cusco, southern peru, where falciparum malaria had not been reported since 1946. although initial microscopic diagnosis reported only plasmodium vivax infection in each of the specimens, subsequent examination by the national reference laboratory confirmed p. falciparum infection in all samples. molecular typing of four available isolates revealed identity as the b-variant (bv1) strain that was responsible for a m ...201526483121
systematics of the oswaldoi complex (anopheles, nyssorhynchus) in south america.effective malaria control relies on accurate identification of those anopheles mosquitoes responsible for the transmission of plasmodium parasites. anopheles oswaldoi s.l. has been incriminated as a malaria vector in colombia and some localities in brazil, but not ubiquitously throughout its neotropical range. this evidence together with variable morphological characters and genetic differences supports that an. oswaldoi s.l. compromises a species complex. the recent fully integrated redescripti ...201324499562
morphological and molecular characterization of pratylenchus lentis n. sp. (nematoda: pratylenchidae) from sicily.pratylenchus lentis n. sp. parasitizing roots of lentil in sicily, italy, is described and illustrated. the new species is characterized by a relatively high lip region with three annuli, mean stylet length of 16 mum, with anteriorly flattened knobs, cylindrical body with a relatively anterior vulva, large and ovoid spermatheca full of sperm, plump tail with truncate, irregularly annulated terminus, and by the presence of males. molecular its-rflp and sequencing analyses of the new species showe ...019440258
the influence of the area of the serra da mesa hydroelectric plant, state of goiás, on the frequency and diversity of anophelines (diptera: culicidae): a study on the effect of a reservoir.bioecological aspects of anophelines (diptera: culicidae) near areas under the direct influence of the hydroelectric plant reservoir of serra da mesa in goiás, brazil, were analyzed.201525860461
identification of blood meals from potential arbovirus mosquito vectors in the peruvian amazon basin.the transmission dynamics of many arboviruses in the amazon basin region have not been fully elucidated, including the vectors and natural reservoir hosts. identification of blood meal sources in field-caught mosquitoes could yield information for identifying potential arbovirus vertebrate hosts. we identified blood meal sources in 131 mosquitoes collected from areas endemic for arboviruses in the peruvian department of loreto by sequencing polymerase chain reaction amplicons of the cytochrome b ...201627621304
geometric morphometrics for the taxonomy of 11 species of anopheles (nyssorhynchus) mosquitoes.the subgenus anopheles (nyssorhynchus) (diptera: culicidae) includes the primary vectors of plasmodium spp. in colombia. most adult females of this subgenus are difficult to identify in the field using the available keys. with the objective of further investigating the discriminatory power of modern morphometrics, both landmark-based and outline-based approaches were explored using the wing venation geometry of 11 anopheles (nyssorhynchus) species. wing shape was able to separate the closest spe ...201525393150
genetic variability among anopheles species belonging to the nyssorhynchus and anopheles subgenera in the amazon region.isoenzymatic analyses were performed involving species of the nyssorhynchus and anopheles subgenera in order to estimate the intra and interspecies genetic variability.201120802941
a survey of adult anophelines in french guiana: enhanced descriptions of species distribution and biting responses.in french guiana, anopheles darlingi is considered the main malaria vector. however, several reports have hypothesized the implication of other anopheline species in malaria transmission for the territory. data on the ecology of these other potential vectors is rare or even unexplored in french guiana. the aim of this study was to describe the biting habits of several anopheline species in multiple localities in french guiana. six sampling sites yielded 1,083 anopheline adults. results indicated ...201324581346
sequence analysis of the second internal transcribed spacer of ribosomal dna in anopheles oswaldoi (diptera: culicidae).sequence divergence in the second internal transcribed spacer (its2) of ribosomal dna was examined for female specimens of anopheles oswaldoi peryassu from 7 localities in south america. the lengths of its2 for all mosquitoes ranged from 348 to 356 nucleotides. after alignment of these sequences, similarity ranged from 87 to 100%. divergence was within the range of inter-specific differences for members of anopheline species complexes. therefore, specimens were placed into 4 groups that may corr ...199910593066
incrimination of anopheles (anopheles) intermedius peryassú, an. (nyssorhynchus) nuneztovari gabaldón, an. (nys.) oswaldoi peryassú as natural vectors of plasmodium falciparum in french guiana.anopheles darlingi root is the major vector of human malaria in the neotropics and has been considered to be the sole malaria vector in french guiana. the presence of other potential vectors suggests that malaria may be transmitted by other species under certain conditions. from 2006-2011, all anopheline specimens collected from 11 localities were assayed to determine if the plasmodium circumsporozoite protein was present. in addition to an. darlingi, we found anopheles oswaldoi, anopheles inter ...201222510842
characterization of larval habitats of anopheles albimanus, anopheles pseudopunctipennis, anopheles punctimacula, and anopheles oswaldoi s.l. populations in lowland and highland ecuador.recent collection data indicate that at least four potential malaria vectors occupy more widespread distributions within the andean highlands than in the past. since habitat elimination is an important aspect of malaria control, it is vital to characterize larval habitats for anopheles species within both lowland and highland sites. to that end, 276 sites within ecuador were surveyed between 2008 and 2010. characteristics of anopheles-present sites for four species were compared to anopheles-abs ...201222548546
an evaluation of the suitability of coi and coii gene variation for reconstructing the phylogeny of, and identifying cryptic species in, anopheline mosquitoes (diptera culicidae).we assessed the practicality and effectiveness of using variation in the mitochondrial coi and coii genes to discriminate species and reconstruct the phylogeny of anophelene mosquitoes. phylogenetic relationships among the subfamily anophelinae were inferred from portions of the mitochondrial coi (92 species) and coii genes (108 species). phylogenetic trees were reconstructed on the basis of parsimony, maximum likelihood and bayesian methods. the suitability of coi and coii gene variation for id ...201627937065
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