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characterization and identification of coagulase-negative, heat-stable deoxyribonuclease-positive staphylococci.various characteristics of 13 coagulase-negative, weakly heat-stable deoxyribonuclease-positive staphylococci from human, veterinary and food sources were determined in an effort to identify them. nine of the isolates were identified as coagulase-negative staphylococcus aureus (2), staphylococcus xylosus (2), staphylococcus simulans (3), staphylococcus capitis (1) and staphylococcus sciuri subsp. lentus (1); the other four isolates, from food and veterinary sources, could not be identified as cu ...1978641525
purification of equine neutrophil lysozyme and its antibacterial activity against gram-positive and gram-negative bacteria.lysozyme from equine neutrophil granulocytes was isolated in a pure form by fast performance liquid chromatography, i.e. ion-exchange chromatography and reversed-phase chromatography. the lysozyme lysed micrococcus luteus, bacillus subtilis and staphylococcus lentus and was also bactericidal against the gram-negative bacteria escherichia coli, klebsiella pneumoniae, bordetella bronchiseptica, and serratia marcescens. staphylococcus aureus and staphylococcus epidermidis were not lysed. the lysozy ...19911803722
comparative characteristics of staphylococcus sciuri, staphylococcus lentus and staphylococcus gallinarum isolated from healthy and sick hosts.of 136 strains of coagulase-negative staphylococci isolated from healthy and sick human beings, goats, sheep, antelope and other animals, 88 (64.7%) were staphylococcus sciuri and 35 (25.7%) were s. lentus and the remainder staphylococcus gallinarum. the strains of s. sciuri were isolated from humans with boils and wounds, goats with pestes des petits ruminants (ppr) and dogs with nasal discharge. one isolate of s. gallinarum came from a fowl with chronic respiratory disease and 11 others were i ...19863705446
staphylococcus sciuri subsp lentus associated with goat mastitis.an organism recovered from mastitic milks of goats was identified as staphylococcus sciuri subsp lentus. isolation of this organism in pure cultures from individual half-udder samples of 5 goats of 18 affected, confirms that it possesses pathogenic potential.19846497107
identification of the staphylococcus sciuri species group with ecori fragments containing rrna sequences and description of staphylococcus vitulus sp. nov.strains of a new species, staphylococcus vitulus, were isolated from food and a variety of mammals. this species was recognized on the basis of the results of an analysis of genomic ecori fragments containing portions of the rrna operons. the patterns of hybridized fragments obtained from strains belonging to the new taxon were sorted into a distinguishable cluster and were distinct from the staphylococcus lentus and staphylococcus sciuri patterns. the results of dna-dna hybridization reactions ...19947520736
bacterial flora of semen collected from danish warmblood stallions by artificial vagina.semen samples were collected from 21 danish warmblood stallions by the colorado artificial vagina (colorado av, 14 samples) or by the missouri artificial vagina (missouri av, 7 samples). the semen was examined bacteriologically by direct plating (dp) on blood agar plates, and by plating of semen swabs stored in stuart's transport media (tm) at 4 degrees c for 1-4 days. no significant differences were observed between results obtained by dp and cultures of identical tm samples. of the 21 samples ...19957572447
emerging chloramphenicol resistance in staphylococcus lentus from mink following chloramphenicol treatment: characterisation of the resistance genes.a total of 26 staphylococcal strains isolated from mink with urinary tract infections as well as from the environment of the mink were examined for antibiotic resistance and prevalence of plasmids mediating resistance to the antibiotics applied for prophylactic or therapeutic purposes. chloramphenicol resistance (cmr) which occurred in fourteen of the eighteen staphylococcus lentus strains, but in none of the staphylococcus intermedius and staphylococcus xylosus strains, was shown to be mediated ...19947801525
characterization of the most frequently encountered staphylococcus sp. in cats.ninety three staphylococci isolated from clinical specimens from cats were characterized and identified. because the biochemical characteristics of staphylococcus felis were very similar to those of staphylococcus simulans, results were submitted to numerical analysis and dna homology. forty-two isolates (45%) were identified as s. felis, and 4 isolates (4%) as s. simulans. the other species identified, in order of their frequency were, 12 staphylococcus aureus (13%), 9 staphylococcus intermediu ...19948042273
antimicrobial susceptibility of ruminal coagulase-negative staphylococci.the species staphylococcus sciuri subsp. sciuri, staphylococcus sciuri subsp. lentus, staphylococcus saprophyticus and staphylococcus xylosus of novobiocin resistant coagulase-negative staphylococci (cns) isolated from rumen content or wall of lambs and calves were determined. all bacteria were tested for lactic acid production, urease, adherence activities and antimicrobial susceptibility or resistance. the majority of strains presented belonged to bacteria with low affinity for epithelial cell ...19948065269
macrolide-lincosamide-streptogramin b resistance in staphylococcus lentus results from the integration of part of a transposon into a small plasmid.the 8.0-kb macrolide-lincosamide-streptogramin b resistance plasmid pses20 from staphylococcus lentus harbored part of a tn917-like transposon including the left terminal repeat, a gene almost identical to ermb, and its regulatory region, as well as the internal direct repeat. homology between pses20 and tn917 ended at a sequence closely related to those of the resolution sites of tn917 and tn552 and staphylococcal recombination sites.19968878614
delimiting the genus staphylococcus through description of macrococcus caseolyticus gen. nov., comb. nov. and macrococcus equipercicus sp. nov., and macrococcus bovicus sp. no. and macrococcus carouselicus sp. nov.four species of the newly proposed genus macrococcus, namely macrococcus caseolyticus gen. nov., comb. nov. (formerly staphylococcus caseolyticus schleifer, kilpper-bälz, fischer, faller and endl 1982, 19vp), macrococcus equipercicus sp. nov., macrococcus bovicus sp. nov. macrococcus carouselicus sp. nov., are described on the basis of a phylogenetic analysis comparing 16s rrna sequences, dna-dna liquid hybridization, dna base composition, normalized ribotype patterns, macrorestriction pattern a ...19989734040
gram positive bacteria induce il-6 and il-8 production in human alveolar macrophages and epithelial cells.inhalation of dust from swine confinement buildings results in an acute inflammatory reaction in the respiratory tract. the dust has a high microbial content, dominated by gram positive bacteria. the aim of the present study was to evaluate the significance of bacteria in the induction of il-6 and il-8 release from respiratory epithelial cells and alveolar macrophages. the results would give an indication to what extent the bacteria contribute to the toxic inflammation following exposure to swin ...199910392756
use of oleic acid to reduce the population of the bacterial flora of poultry skin.the effect of oleic acid on native bacterial flora of poultry skin was examined. skin from commercial broiler carcasses was washed once or twice in solutions of 0, 2, 4, 6, 8, or 10% (wt/vol) oleic acid and rinsed in peptone water. aerobic bacteria, enterobacteriaceae, campylobacter, and enterococci in the rinsates were enumerated. significantly fewer aerobic bacteria, enterobacteriaceae, campylobacter, and enterococci were recovered from rinsates of skin washed in oleic acid than from control s ...200010983807
detection and rapid increase of salivary antibodies to staphylococcus lentus, an indigenous bacterium in rabbit saliva, through a single tonsillar application of bacterial cells.in rabbits, staphylococcus lentus is one of the major bacteria in saliva and a minor bacteria in jejunum fluids and nasal wash. the presence and induction of naturally occurring antibodies reacting to rabbit indigenous bacteria were studied. in non-immune rabbits, the proportion of anti-s. lentus iga antibodies to total iga in the saliva was several times higher than that in the intestinal fluids and the nasal wash. the salivary antibodies were found to have increased 1 week after a single tonsi ...200111555301
antimicrobial susceptibility of staphylococci isolated from the faeces of wild turkeys (meleagris gallopavo).the purpose of this study was to investigate the staphylococcal flora associated with wild turkey populations.200111696101
distribution of meca-harboring staphylococci in healthy mares.the prevalence of staphylococci that harbor the meca gene responsible for methicillin resistance was examined in healthy breeding mares. staphylococci often cause diseases of horses such as metritis, keratitis, and abscess. methicillin-resistant staphylococci would make antibiotic treatments ineffective, so it may be significant to know the distribution of meca-harboring staphylococci in mares. isolation of meca-harboring staphylococci was achieved from nares and pasterns of 100 mares in hokkaid ...200212399608
identification and isolation of a bactericidal domain in chicken egg white lysozyme.chicken egg white lysozyme exhibits antimicrobial activity against both gram-positive and gram-negative bacteria. fractionation of clostripain-digested lysozyme yielded a pentadecapeptide with antimicrobial activity but without muramidase activity. the peptide was isolated and its sequence found to be i-v-s-d-g-n-g-m-n-a-w-v-a-w-r (amino acids 98-112 of chicken egg white lysozyme). a synthesized peptide of identical sequence had the same bactericidal activity as the natural peptide. replacement ...199712455902
occurrence of resistance to antibiotics, metals, and plasmids in clinical strains of staphylococcus spp.presence of staphylococci resistant to antibiotics is of concern, as is the possible spread of resistance determinants among staphylococcus species. during the past several years, a series of staphylococcal isolates that demonstrated reduced susceptibility to vancomycin or other glycopeptides has been reported. however, there are no useful data on species sensitivity of coagulase-negative staphylococci to heavy metals.200312700009
antibacterial activity of casein-derived peptides isolated from rabbit (oryctolagus cuniculus) milk.acid-precipitated rabbit 'whole casein' was digested by trypsin, chymotrypsin, pepsin, and clostripain to screen for possible peptides with antibacterial properties. the peptide fragments were separated by reversed-phase chromatography. the collected fractions were pooled and their antibacterial properties tested against escherichia coli, bacillus subtilis and staphylococcus lentus. three antibacterial peptide fragments derived from tryptic digestion of rabbit casein were isolated and identified ...200312800873
screening of bifidobacterium strains isolated from human faeces for antagonistic activities against potentially bacterial pathogens.as probiotic bacteria, strains belonging to the genus bifidobacterium colonise the gastro-intestinal tract of humans and animals at the time of birth, and they are found in young as well as in adult individuals in great numbers. moreover, they can interact with the development of enteric infections by the production of antimicrobial metabolites. in this work 281 strains of bifidobacteria were anaerobically isolated from human faecal samples, supplied by volunteers of different ages (youngs, adul ...200312906392
fexa, a novel staphylococcus lentus gene encoding resistance to florfenicol and chloramphenicol.the staphylococcus lentus plasmid pscfs2 carries a novel florfenicol-chloramphenicol resistance gene, designated fexa, encoding a protein of 475 amino acids with 14 transmembrane domains. the fexa protein differs from all previously known proteins involved in the efflux of chloramphenicol and florfenicol. induction of fexa expression by chloramphenicol and florfenicol occurs via translational attenuation.200414742219
florfenicol-chloramphenicol exporter gene fexa is part of the novel transposon tn558.the florfenicol-chloramphenicol exporter gene fexa is part of the novel transposon tn558 from staphylococcus lentus. similarities between tn558 and tn554 from staphylococcus aureus included the arrangement of the transposase genes tnpa to -c and an att554-like target sequence. circular forms of tn558 were detected and suggest the functional activity of this transposon.200515673776
tlr-2 is involved in airway epithelial cell response to air pollution particles.primary cultures of normal human airway epithelial cells (nhbe) respond to ambient air pollution particulate matter (pm) by increased production of the cytokine il-8, and the induction of several oxidant stress response genes. components of ambient air pm responsible for stimulating epithelial cells have not been conclusively identified, although metal contaminants, benzo[a]pyrene and biological matter have been implicated. stimulation of il-8 release from nhbe with coarse (pm(2.5-10)), fine (pm ...200515694463
staphylococcal tetracycline-mlsb resistance plasmid pste2 is the product of an rsa-mediated in vivo recombination.the complete nucleotide sequence of the 6913 bp plasmid pste2 from staphylococcus lentus, which mediates inducible resistance to tetracyclines, macrolides and lincosamides, was determined. the plasmid was analysed for potential reading frames and structural features to gain insight into its development from potential ancestor plasmids.200515980097
bacterial decontamination of duwl biofilm using oxygenal 6.the aim of this study was bacteriological assessment of the dental unit waterlines (duwl) biofilm - concentration and composition of the aerobe and facultative anaerobe bacterial microflora, and evaluation of the influence of a disinfecting product, oxygenal 6, on the biofilm composition. tubing fragments were taken from 25 units twice, before and after disinfection, and bacterial suspension of the biofilm was obtained from the samples. the bacterial flora was determined with the plate culture m ...200616841887
splenic abscess due to staphylococcus lentus: a rare entity.splenic abscess is an unusual condition, most often seen in immunocompromised patients. splenic abscesses are due to aerobic and anaerobic bacteria, fungi and parasites, staphylococcus aureus being the most common aetiological agent. we describe a case of splenic abscess in an alcoholic patient due to staphylococcus lentus, a rare aetiology included in the microbiology of this disease.200616857621
supplementary biochemical tests useful for the differentiation of oxidase positive staphylococci.differentiation of the oxidase positive staphylococci, staphylococcus sciuri, staphylococcus lentus, staphylococcus vitulinus and staphylococcus fleurettii, based on tributyrin, urease, caseinase, gelatinase and dnase activity is described. these tests may be used for preliminary identification of oxidase positive isolates of staphylococci resulting in more accurate identification of these species.200717207599
microbiological changes in naturally fermented cassava fish (pseudotolithus sp.) for lanhouin production.cassava fish (pseudotolithus sp.) was naturally fermented on three different occasions at room temperature (28-30 degrees c) for 3 to 8 days and the microbial population and the occurrence of various bacterial species were monitored. in general, after a slight increase during the early stages of fermentation, the microbial population decreased as the fermentation progressed. a total of 224 isolates belonging to the genera bacillus, staphylococcus, micrococcus, streptococcus, corynebacterim, pseu ...200717291615
antibiotic resistance patterns of coagulase-negative staphylococcus strains isolated from blood cultures of septicemic patients in turkey.the aim of this study is to determine antibiotic resistance patterns and slime production characteristics of coagulase-negative staphylococci (cons) caused nosocomial bacteremia. a total of 200 cons strains were isolated from blood samples of patients with true bacteremia who were hospitalized in intensive care units and in other departments of istanbul university cerrahpasa medical hospital between 1999 and 2006. among 200 cons isolates, staphylococcus epidermidis was the most prevalent species ...200917475456
[exposure assessment to bioaerosols among sewer workers].the purpose of the study was to evaluate exposure to bioaerosols among sewer workers.200617533989
medicinal smoke reduces airborne bacteria.this study represents a comprehensive analysis and scientific validation of our ancient knowledge about the effect of ethnopharmacological aspects of natural products' smoke for therapy and health care on airborne bacterial composition and dynamics, using the biolog microplate panels and microlog database. we have observed that 1h treatment of medicinal smoke emanated by burning wood and a mixture of odoriferous and medicinal herbs (havan sámagri=material used in oblation to fire all over india) ...200717913417
antibiotic resistance genes and identification of staphylococci collected from the production chain of swine meat commodities.staphylococci harbouring antibiotic resistance (ar) genes may represent a hazard for human health and, as other resistant food-related bacteria, they contribute to the spread of ar. in this study, we isolated resistant staphylococci from an entire swine production chain and investigated the occurrence of 11 genes [aac(6')ie-aph(2'')ia, blaz, meca, vana, vanb, erma, ermb, ermc, tet(m), tet(o) and tet(k)] encoding resistance to some antibiotics largely used in clinical practice. the 66 resistant s ...200817993395
antimicrobial resistance and heat sensitivity of oxacillin-resistant, meca-positive staphylococcus spp. from unpasteurized milk.eight staphylococcus spp. carrying the meca gene were isolated from oxacillin enrichments of 70 unpasteurized milk samples. the isolates were identified as five staphylococcus epidermidis, two staphylococcus lentus, and one staphylococcus haemolyticus. no meca-positive staphylococcus aureus were isolated. all isolates carried genes for other antibiotic resistances in addition to meca. the results establish that meca-carrying coagulase-negative staphylococcus spp. in unpasteurized milk have the p ...200818236682
subinhibitory concentrations of antibiotics induce phenazine production in a marine streptomyces sp.subinhibitory concentrations of antibiotics were found to enhance and modulate the production of new phenazines, streptophenazines a-h, in a marine streptomyces isolate. the streptophenazines differ in length and substitution of an alkyl chain. the pattern of metabolites formed depends on the antibiotic used. in the presence of tetracycline, streptophenazines f and g were induced and the production of streptophenazines a-d was increased. when using bacitracin, mainly streptophenazine h was produ ...200818396903
diversity of antibiotic-active bacteria associated with the brown alga laminaria saccharina from the baltic sea.bacteria associated with the marine macroalga laminaria saccharina, collected from the kiel fjord (baltic sea, germany), were isolated and tested for antimicrobial activity. from a total of 210 isolates, 103 strains inhibited the growth of at least one microorganism from the test panel including gram-negative and gram-positive bacteria as well as a yeast. most common profiles were the inhibition of bacillus subtilis only (30%), b. subtilis and staphylococcus lentus (25%), and b. subtilis, s. len ...200918855068
antioxidant and antimicrobial effects of phenolic compounds extracts of northeast portugal honey.phenolic compounds of dark and clear honeys from trás-os-montes of portugal were extracted with amberlite xad-2 and evaluated for their antioxidant and antimicrobial activities. the antioxidant effect was studied using the in vitro test capacity of scavenge the 2,2-diphenyl-1-picryhydrazyl (dpph) free radical and of reducing power of iron (iii)/ferricyanide complex. the antimicrobial activity was screened using three gram-positive bacteria (bacillus subtilis, staphylococcus aureus, staphylococcu ...200818940227
response to somatic cell count-based selection for mastitis resistance in a divergent selection experiment in sheep.a divergent selection experiment in sheep was implemented to study the consequences of log-transformed somatic cell score (scs)-based selection on resistance to natural intramammary infections. using dams and progeny-tested rams selected for extreme breeding values for scs, we created 2 groups of ewes with a strong divergence in scs of approximately 3 genetic standard deviations. a survey of 84 first-lactation ewes of both the high and low scs lines indicated favorable responses to scs-based sel ...200919233814
investigation of bacteria with polyketide synthase genes and antimicrobial activity isolated from south china sea sponges.to obtain bacteria with pks (polyketide synthase) genes and antimicrobial activity from sponges.200919302490
diversity of staphylococcal cassette chromosome in coagulase-negative staphylococci from animal sources.to type the staphylococcal cassette chromosome (scc) in coagulase-negative staphylococci (cons) from animal sources.200919486384
characterization of staphylococcus species isolated from raw milk and milk products (lben and jben) in north morocco.to investigate the incidence and antibiotic resistance of staphylococcal strains isolated from milk and milk products and to trace the ecological origin of the staphylococcus aureus isolated.200819738354
occurrence of bacteria of the genus staphylococcus in table eggs descended from different sources.this paper presents the degree of contamination of table eggs with bacteria of the genus staphylococcus, taking into account the source of the eggs. the results of the study indicate a relatively high degree of contamination of table eggs with staphylococcus bacteria. in 1125 bacteriological tests conducted on whites, yolks and shells of eggs from three sources, staphylococci were found in 514 cases. thirteen strains were isolated from the whites, but staphylococcus bacteria were found more ofte ...200920169921
comparison of phenotypic methods in predicting methicillin resistance in coagulase-negative staphylococcus (cons) from animals.phenotypic detection of methicillin resistance in coagulase-negative staphylococcus (cons) of animal origin has been challenging due to the heterogeneous expression of meca. to compare different phenotypic methods in predicting the meca presence in cons, a total of 87 cons isolates from agricultural animals were analyzed in this study by agar dilution, disk diffusion, and broth microdilution. meca was present in 81 cons isolates. broth microdilution demonstrated the highest sensitivity of 100% i ...201120538309
phage control of dual species biofilms of pseudomonas fluorescens and staphylococcus lentus.despite the recent enthusiasm for using bacteriophages as bacterial control agents, there are only limited studies concerning phage interaction with their respective hosts residing in mixed biofilm consortia and especially in biofilms where the host species is a minor constituent. in the present work, a study was made of mono and dual species biofilms formed by pseudomonas fluorescens (gram-negative) and/or staphylococcus lentus (gram-positive) and their fate after infection with phages. the dua ...201020544433
dominant bacterial communities in the rumen of gayals (bos frontalis), yaks (bos grunniens) and yunnan yellow cattle (bos taurs) revealed by denaturing gradient gel electrophoresis.the dominant rumen bacteria in gayals, yaks and yunnan yellow cattle were investigated using pcr-dgge approach. the analysis of dgge profiles, identification of dominant bands and phylogenetic analysis 16s rdna sequences in dgge profiles were combined to reveal the dominant bacterial communities and compared the differences between those cattle species. dgge profiles revealed that gayals had the most abundant dominant bacteria and the lowest similarity of intraspecies between individuals than ot ...201021140219
catalase-positive cocci in fermented sausage: variability due to different pork breeds, breeding systems and sausage production technology.the aim of this study was to compare the ecology of catalase-positive cocci (cpc) present in traditional fermented sausages produced using different breeds of pork, each of which was raised in two different environments and processed using two different technologies. semi-quantitative molecular methods were used to determine bacterial identities. almost all fermentations were characterised by a significant increase in cpc during the first few days of fermentation, reaching values of 10(5)-10(6)  ...201222202871
[diversity of cultured actinomycete in the baltic sea].actinomycetes (actinobacteria) are getting more and more recognized as a natural source for new drug exploration. in order to find new lead compounds, the diversity and selected bioactivities of cultured actinomycetes in the baltic sea (germany) were investigated.201122260043
biodiversity and characterization of indigenous coagulase-negative staphylococci isolated from raw milk and cheese of north italy.the aim of the current study was to detect coagulase-negative staphylococci (cns) in raw milk and cheeses produced in north italy, and to analyze isolates for their biodiversity, safety aspects and technological properties. molecular identification methods revealed a high biodiversity among isolates and assigned them to 17 species. the most recovered species were staphylococcus equorum (12%), staphylococcus lentus (12%), staphylococcus simulans (12%), staphylococcus sciuri (10%), and staphylococ ...201223498185
[diversity and bioactivity of culturable actinobacteria from animal feces].in order to provide new source for discovering new lead compounds of drugs and other products, the diversity and some bioactivities of culturable actinobacteria in animal feces were studied.201223289327
conjunctival flora of clinically normal and diseased turtles and tortoises.in captive breed turtles and tortoises conjunctival disease is common. our aim was to investigate the bacterial and fungal flora present in the eyes of healthy and pathological chelonians and to compare findings in turtles with those in tortoises.201525889261
identification of causative pathogens in mouse eyes with bacterial keratitis by sequence analysis of 16s rdna libraries.the clone library method using pcr amplification of the 16s ribosomal rna (rrna) gene was used to identify pathogens from corneal scrapings of c57bl/6-corneal opacity (b6-co) mice with bacterial keratitis. all 10 samples from the eyes with bacterial keratitis showed positive pcr results. all 10 samples from the normal cornea showed negative pcr results. in all 10 pcr-positive samples, the predominant and second most predominant species accounted for 20.9 to 40.6% and 14.7 to 26.1%, respectively, ...201425312507
microbiological stability of canned tuna produced in italy and in non-european countries.the results of a study on the microbiological stability of canned tuna produced by italian companies and similar canned products manufactured in countries outside europe are reported herein. the study involved 38 samples of canned tuna of various brands, of which 14 were produced by companies outside europe and 24 by italian companies. qualitative and quantitative microbiological tests were conducted for the following parameters: bacterial colony counts at 30°c, total coliforms, total enterobact ...201527800383
molecular identification of microbial community in surface and undersurface douchi during postfermentation.to find the reason for fermentation failure of surface douchi during postfermentation, the microbial communities in undersurface and surface samples were investigated using cell counting method and denaturing gradient gel electrophoresis (dgge). the results showed that the microbial biomass in surface douchi was obviously different from that in undersurface douchi even sampled from the same fermentation tanks, and a 10- to 100-fold reduction of microbial cell counts in undersurface had been obse ...201424621312
elaiomycins k and l, new azoxy antibiotics from streptomyces sp. tü 6399*.elaiomycins k and l, two new azoxy-type antibiotics, were detected by hplc-diode array screening in the culture filtrate extract of streptomyces sp. tü 6399. the structures were determined by high-resolution ms and 2-dimensional (1)h and (13)c correlated nmr spectroscopy including (15)n-nmr experiments and established these compounds as new members of the elaiomycin family. both metabolites show a weak antibacterial activity against bacillus subtilis and staphylococcus lentus as well as against ...201323149516
unique hyper-thermal composting process in kagoshima city forms distinct bacterial community structures.a unique compost, satsuma soil, is produced from three types of wastewater sludge using hyper-thermal processes at temperatures much higher than that of general thermophilic processes in kagoshima city, japan. we analyzed the bacterial community structures of this hyper-thermal compost sample and other sludges and composts by a high-throughput barcoded pyrosequencing method targeting the 16s rrna gene. in total, 621,076 reads were derived from 17 samples and filtered. artificial sequences were d ...201627212266
isolation and characterization of three benzylisoquinoline alkaloids from thalictrum minus l. and their antibacterial activity against bovine mastitis.the roots of thalictrum minus are traditionally used in the treatment of inflammation and infectious diseases such as bovine mastitis. however, there are no reports available in literature till date regarding the antibacterial studies of t. minus against bovine mastitis.201627426505
co-location of the oxazolidinone resistance genes optra and cfr on a multiresistance plasmid from staphylococcus sciuri.to identify and characterize the oxazolidinone/phenicol resistance gene optra in staphylococcus isolates.201626953332
distribution of cfr in staphylococcus spp. and escherichia coli strains from pig farms in china and characterization of a novel cfr-carrying f43:a-:b- plasmid.the multi-resistance gene cfr is widely distributed among various gram-positive and gram-negative species in livestock in china. to better understand the epidemiology of cfr among staphylococcus spp. and e. coli isolates, 254 staphylococcus spp. and 398 e. coli strains collected from six swine farms in china were subjected to prevalence and genetic analysis. forty (15.7%) staphylococcus spp. isolates, including 38 staphylococcus sciuri strains, one staphylococcus chromogenes strain, and one stap ...201728293235
high throughput genomic sequencing of bioaerosols in broiler chicken production facilities.chronic inhalation exposure to agricultural dust promotes the development of chronic respiratory diseases among poultry workers. poultry dust is composed of dander, chicken feed, litter bedding and microbes. however, the microbial composition and abundance has not been fully elucidated. genomic dna was extracted from settled dust and personal inhalable dust collected while performing litter sampling or mortality collection tasks. dna libraries were sequenced using a paired-end sequencing-by-synt ...201627470660
dissemination of a pscfs3-like cfr-carrying plasmid in staphylococcus aureus and staphylococcus epidermidis clinical isolates recovered from hospitals in ohio.nineteen linezolid-resistant staphylococcus epidermidis and two staphylococcus aureus isolates recovered from two medical institutions in northeast ohio and an s. aureus cfr index strain previously collected in the same facilities during the 2007 sentry antimicrobial surveillance program were investigated for the genetic basis of oxazolidinone resistance and the location of cfr. s. aureus isolates were typed by pulsed-field gel electrophoresis (pfge), spa typing, and multilocus sequence typing ( ...201323571552
occurrence of cfr-mediated multiresistance in staphylococci from veal calves and pigs, from humans at the corresponding farms, and from veterinarians and their family members.this study reports on the emergence of linezolid-resistant coagulase-negative staphylococci (cons) containing the multiresistance gene cfr in veal calves and pigs, as well as in humans exposed to these animals. cons (staphylococcus auricularis, staphylococcus cohnii, staphylococcus lentus, staphylococcus kloosii, staphylococcus sciuri, staphylococcus simulans), but not staphylococcus aureus, carrying the gene cfr were detected in samples of 12 out of 52 calves at three farms which had a history ...201727102205
characterization of staphylococci in urban wastewater treatment plants in spain, with detection of methicillin resistant staphylococcus aureus st398.the objective of this study was to determine the prevalence of staphylococcus in urban wastewater treatment plants (uwtp) of la rioja (spain), and to characterize de obtained isolates. 16 wastewater samples (8 influent, 8 effluent) of six uwtps were seeded on mannitol-salt-agar and oxacillin-resistance-screening-agar-base for staphylococci and methicillin-resistant staphylococcus aureus recovery. antimicrobial susceptibility profile was determined for 16 antibiotics and the presence of 35 antimi ...201626840519
identification of abc transporter genes conferring combined pleuromutilin-lincosamide-streptogramin a resistance in bovine methicillin-resistant staphylococcus aureus and coagulase-negative staphylococci.the aim of this study was to investigate the genetic basis of combined pleuromutilin-lincosamide-streptogramin a resistance in 26 unrelated methicillin-resistant staphylococcus aureus (mrsa) and coagulase-negative staphylococci (cons) from dairy cows suffering from mastitis. the 26 pleuromutilin-resistant staphylococcal isolates were screened for the presence of the genes vga(a), vga(b), vga(c), vga(e), vga(e) variant, sal(a), vmlr, cfr, lsa(a), lsa(b), lsa(c), and lsa(e) by pcr. none of the 26 ...201525891423
the ecological importance of the staphylococcus sciuri species group as a reservoir for resistance and virulence genes.the staphylococcus sciuri species group includes five species that are most often presented as commensal animal-associated bacteria. the species of this group are staphylococcus sciuri (with three subspecies), staphylococcus lentus, staphylococcus vitulinus, staphylococcus fleurettii and staphylococcus stepanovicii. members of these group are commonly found in a broad range of habitats including animals, humans and the environment. however, those species have been isolated also from infections, ...201424629775
the oral microbiota of domestic cats harbors a wide variety of staphylococcus species with zoonotic potential.this study aimed to characterize the species, antimicrobial resistance and dispersion of crispr systems in staphylococci isolated from the oropharynx of domestic cats in brazil. staphylococcus strains (n=75) were identified by maldi-tof and sequencing of rpob and tuf genes. antimicrobial susceptibility was assessed by disk diffusion method and pcr to investigate the presence of antimicrobial-resistance genes usually present in mobile genetic elements (plasmids), in addition to plasmid extraction ...201728284599
draft genome sequence of a multiresistant bovine isolate of staphylococcus lentus from tanzania.we report here the draft genome sequence of a staphylococcus lentus isolate, 050ap, collected in tanzania from a swab of healthy bovine perineum. the draft genome sequence contained 2.72 mbp and 2,750 coding sequences with a g+c content of 31.7%.201627908999
resistance behaviour of inducible clindamycin-resistant staphylococci from clinical samples and aquatic environments.in this study, the species diversity of staphylococci with inducible resistance to macrolides, lincosamides and streptogramin b (mlsb) isolated from clinical samples, sewage and river water was investigated. inducible clindamycin resistance was tested using a d-test and macrodilution assays. inducible cross-resistance (imlsb phenotype) was examined by pcr of erm gene classes a, b, c, f, g, q, t and 43. although ermc was the most frequently detected resistance gene in imlsb phenotypes of environm ...201425106860
staphylococcus lentus peritonitis: a case report. 201424991057
isolation and molecular characterization of methicillin-resistant staphylococci from horses, personnel and environmental sites at an equine hospital in turkey.the present study was carried out to assess the frequency of methicillin-resistant staphylococci (mrs) among racehorses (n=209) and veterinary personnel (n=13) as well as environmental surfaces (n=14) at an equine hospital in adana, turkey. in addition, species distribution, antimicrobial susceptibility, resistance genes, staphylococcal chromosomal cassette mec (sccmec) type and clonality of these isolates were also investigated. mrs were identified by 16s rrna sequencing, and typed by pulsed-fi ...201222820519
bioenergetics for the growth of staphylococcus lentus in biocompatible choline salts.choline-based biocompatible salts were used as "nutrients" for the growth of staphylococcus lentus bacteria. increase in the growth rate of bacteria was observed, compared to conventional carbon sources. in the case of the ionic liquid, choline lactate, the increase was pronounced. bacterial growth was correlated with power-time curve in an investigation monitored online by reaction calorimetry. from the power-time curve, three phases of the growth can be distinctly seen. heat yield coefficients ...201322526779
phytate-degrading enzyme production by bacteria isolated from malaysian soil.over two hundred bacteria were isolated from the halosphere, rhizosphere and endophyte of malaysian maize plantation and screened for phytases activity. thirty isolates with high detectable phytase activity were chosen for media optimization study and species identification. ten types of bacterial phytase producers have been discovered in this study, which provides opportunity for characterization of new phytase(s) and various commercial and environmental applications. the majority of the bacter ...200727517819
bacterial contamination in physical therapy departments in the state of kuwait.[purpose] interferential therapy and electrical stimulation are electrophysical modalities commonly used in physical therapy departments to treat patients with musculoskeletal problems. these machines are applied directly to the patient's skin via a medium or electrodes, which can facilitate the transmission of microorganisms from one patient to another. the purpose of this study was to determine the extent of microorganism contamination in the machines sponges at physical therapy departments in ...201728626312
elevation of staphylococcus sciuri subsp. lentus (kloos et al.) to species status: staphylococcus lentus (kloos et al.) comb. nov.deoxyribonucleic acid (dna)-dna hybridization and physiological studies on 15 strains of s. sciuri subsp. lentus and 4 strains of s. sciuri subsp. sciuri demonstrated that species rank should be conferred on these subspecies. strain atcc 29070, the type strain of s. sciuri subsp. lentus, is proposed as type strain of s. lentus. an emended description of this strain is given.198323194736
bioenergetics and pathway of acid blue 113 degradation by staphylococcus lentus.bioreaction calorimetric studies of degradation of the dye acid blue 113 by staphylococcus lentus are reported for the first time. the heat released during the dye degradation process can be successfully measured using reaction calorimeter. power time and oxygen uptake rate (our) profile followed each other suggesting that heat profiles could monitor the progress of the dye degradation in biocalorimetry. the shifts observed in power-time profile indicated three distinct phases of the bioprocess ...201222927362
genome sequence of staphylococcus lentus f1142, a strain isolated from korean soybean paste.this report describes the draft genome sequence of staphylococcus lentus f1142, which was isolated from a korean fermented soybean paste (doenjang). the draft genome sequence contained 2.79 mbp with a g+c content of 31.8%; this is the first s. lentus genome to be reported.201223045498
the metabolic advantage of choline lactate in growth media: an experimental analysis with staphylococcus lentus.the metabolic effectiveness of choline lactate in the growth media was investigated relative to conventional carbon source for growing staphylococcus lentus, a bacterial strain commonly used in bioremediation of industrial effluents and xenobiotic detoxification. bacterial growth thermodynamics was determined by biocalorimetry. (13)c nmr and ftir spectroscopic analyses traced the consumption of choline lactate at specific time intervals of bacterial growth. under aerobic conditions, it is appare ...201323197345
multidrug-resistant coagulase-negative staphylococci in food animals.to study the antimicrobial resistance of coagulase-negative staphylococci (cons) in animals.201222816491
characterization of methicillin-resistant coagulase-negative staphylococci (mrcons) in retail meat.this study was to understand the extent of methicillin-resistant coagulase-negative staphylococci (mrcons) serving as a meca reservoir in retail meat. mrcons were isolated from retail meat (beef, chicken, and turkey) in detroit and characterized by soda gene sequencing for species identification, staphylococcal cassette chromosome mec (sccmec) typing, and pulsed-field gel electrophoresis (pfge). unique mrcons isolates recovered from 25 meat samples were comprised of staphylococcus sciuri (n = 13 ...201424929717
coagulase-negative staphylococci (cons) isolated from ready-to-eat food of animal origin--phenotypic and genotypic antibiotic resistance.the aim of this work was to study the pheno- and genotypical antimicrobial resistance profile of coagulase negative staphylococci (cons) isolated from 146 ready-to-eat food of animal origin (cheeses, cured meats, sausages, smoked fishes). 58 strains were isolated, they were classified as staphylococcus xylosus (n = 29), staphylococcus epidermidis (n = 16); staphylococcus lentus (n = 7); staphylococcus saprophyticus (n = 4); staphylococcus hyicus (n = 1) and staphylococcus simulans (n = 1) by phe ...201525475289
potential spread of multidrug-resistant coagulase-negative staphylococci through healthcare waste.healthcare waste (hcw) might potentially harbor infective viable microorganisms in sanitary landfills. we investigated the antimicrobial susceptibility patterns and the occurrence of the meca gene in coagulase-negative staphylococcus strains (cons) recovered from the leachate of the hcw in an untreated sanitary landfill.201525596568
short communication: prevalence, antimicrobial resistance, and resistant traits of coagulase-negative staphylococci isolated from cheese samples in turkey.a total of 17 coagulase-negative staphylococci (cns) isolates obtained from 72 cheese samples were included in this study. coagulase-negative staphylococci isolates obtained in this study comprised 6 (35.3%) staphylococcus saprophyticus, 3 (17.6%) staphylococcus epidermidis, 2 (11.8%) staphylococcus hominis, 2 (11.8%) staphylococcus haemolyticus, 1 (5.9%) staphylococcus xylosus, 1 (5.9%) staphylococcus vitulinus, 1 (5.9%) staphylococcus lentus, and 1 (5.9%) staphylococcus warneri. the disc diffu ...201626874419
characterization of mannitol-fermenting methicillin-resistant staphylococci isolated from pigs in nigeria.this study was conducted to determine the species distribution, antimicrobial resistance pheno- and genotypes and virulence traits of mannitol-positive methicillin-resistant staphylococci (mrs) isolated from pigs in nsukka agricultural zone, nigeria. twenty mannitol-positive methicillin-resistant coagulase-negative staphylococcal (mrcons) strains harboring the meca gene were detected among the 64 staphylococcus isolates from 291 pigs. a total of 4 species were identified among the mrcons isolate ...201526413075
new mlsb resistance gene erm(43) in staphylococcus lentus.the search for a specific rrna methylase motif led to the identification of the new macrolide, lincosamide, and streptogramin b resistance gene erm(43) in staphylococcus lentus. an inducible resistance phenotype was demonstrated by cloning and expressing erm(43) and its regulatory region in staphylococcus aureus. the erm(43) gene was detected in two different dna fragments, of 6,230 bp and 1,559 bp, that were each integrated at the same location in the chromosome in several s. lentus isolates of ...201222733067
characterization of methicillin-resistant non-staphylococcus aureus staphylococci carriage isolates from different bovine populations.this study aimed at investigating bovine non-staphylococcus aureus staphylococci for their role as a potential reservoir for methicillin resistance.201323090977
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