genetic differentiation and diagnostic loci among anopheles (nyssorhynchus) rangeli, an. (nys.) nuneztovari, and an. (nys.) dunhami (diptera: culicidae) in the brazilian amazon. | genetic variability and divergence were estimated for populations of anopheles rangeli gabaldón, cova-garcia & lopes, anopheles nuneztovari gabald6n cytotype a, and anophels dunhami causey from the brazilian amazon using isozyme electrophoresis. these species are included in the oswaldoi subgroup, subgenus nyssorhynchus (diptera: culicidae). thirteen enzymes yielded a total of 22 loci, of which 14 were monomorphic in the three species. three diagnostic loci (gpi-1, hk-1, and me) and a strong dif ... | 2002 | 12144292 |
morphometric discrimination of females of five species of anopheles of the subgenus nyssorhynchus from southern and northwest colombia. | the most important vectors of human plasmodium in the neotropics belong to the subgenus nyssorhynchus. these species are generally sympatric in terms of their geographical distributions. some are difficult to identify based solely on examination of adult females using the available morphological keys, in these cases examination of immature stages and male genitalia is required to make correct determinations. however, in epidemiological studies it is necessary to identify the species of adult fem ... | 2003 | 12563488 |
[egg morphology as an indirect method to identify anopheles benarrochi, anopheles oswaldoi and anopheles rangeli (diptera: culicidae)]. | in the department of putumayo in southern colombia, malaria transmission has continued in the absence of the 4 traditional latin american vector species--anopheles darlingi, anopheles nuneztovari, anopheles albimanus or anopheles trinkae. human bait collections yielded anopheles mosquitoes and a morphological variant of anopheles benarrochi, the adult females of which can easily be misidentified as anopheles oswaldoi. species identification of females of anopheles in the subgenus nyssorhynchus i ... | 2003 | 14968916 |
dentification of four vectors of human plasmodium spp. by multiplex pcr: anopheles rangeli, an. strodei, an. triannulatus, and an. trinkae (diptera: culicidae: nyssorhynchus). | one of the major obstacles for studies of the biology, ecology, and behavior of neotropical vectors of human plasmodium has been the lack of reliable and efficient means of identifying many species. although the subgenus nyssorhynchus includes most species responsible for human transmission in south america, there are no polymerase chain reaction (pcr)-based techniques for identifying members of this taxon. we describe the first multiplex pcr for identifying four species in the subgenus nyssorhy ... | 2004 | 15605651 |
biting activity and breeding sites of anopheles species in the municipality villavicencio, meta, colombia. | villavicencio, the capital city of the department of meta, colombia, is at high risk for the urbanization of malaria because of the region's ecological conditions, as well as the permanent presence of infected human populations arriving from rural areas. from august to november 2002 and in april 2003, anopheline collections were undertaken in the area. isofamilies were obtained from 331 wild females, which were then recorded according to their abundance as follows: anopheles marajoara, anopheles ... | 2005 | 16033120 |
morphological analysis of three populations of anopheles (nyssorhynchus) nuneztovari gabaldón (diptera: culicidae) from colombia. | based on the results of comparative analyses of 1,039 specimens of several progenies of anopheles nuneztovarifrom three localities in colombia, eight costal wing spot patterns were observed. patterns i and iii were the most frequent: 77.96% and 11.36%, respectively. using the diagnostic characters ratio of the length of the basal dark area of hind tarsomere ii/length of hind tarsomere ii, ratio of the length of the humeral pale spot/length of the pre-humeral dark spot, and the ratio of the lengt ... | 2008 | 18368239 |
utilization, retention and bio-efficacy studies of permanet in selected villages in buie and fentalie districts of ethiopia. | malaria remains a major public health problem in ethiopia. pyrethroid-treated mosquito nets are one of the major tools available for the prevention and control of malaria transmission. permanet is a long-lasting insecticide-treated net (llin) recommended by who for malaria control. | 2009 | 19480712 |
new report on the bionomics of coquillettidia venezuelensis in temporary breeding sites (diptera: culicidae). | findings of immature forms of coquillettidia venezuelensis in temporary breeding sites, without the presence of aquatic plants or other submerged plant tissue are reported. | 2011 | 21552744 |
the influence of mosquito resting behaviour and associated microclimate for malaria risk. | abstract: | 2011 | 21736735 |
improved molecular technique for the differentiation of neotropical anopheline species. | we evaluated a pcr-rflp of the ribosomal internal transcribed spacer 2 region (its2) to distinguish species of anopheles commonly reported in the amazon and validated this method using reared f1 offspring. the following species of anopheles were used for molecular analysis: an. (nys.) benarrochi, an. (nys.) darlingi, an. (nys.) nuneztovari, an. (nys.) konderi, an. (nys.) rangeli, and an. (nys.) triannulatus sensu lato (s.l.). in addition, three species of the subgenus anopheles, an. (ano.) forat ... | 2008 | 18337348 |
effectiveness of mosquito magnet® trap in rural areas in the southeastern tropical atlantic forest. | traps are widely employed for sampling and monitoring mosquito populations for surveillance, ecological and fauna studies. considering the importance of assessing other technologies for sampling mosquitoes, we addressed the effectiveness of mosquito magnet® independence (mmi) in comparison with those of the cdc trap with co2 and lurex3® (cdc-a) and the cdc light trap (cdc-lt). field collections were performed in a rural area within the atlantic forest biome, southeastern state of são paulo, braz ... | 2014 | 25424445 |
abundance, behavior and entomological inoculation rates of anthropophilic anophelines from a primary colombian malaria endemic area. | in colombia for several years, the urabá-bajo cauca and alto sinú region has registered the highest numbers of malaria cases in the country. malaria vector incrimination and the characterization of entomological parameters will allow for a better understanding of malaria transmission dynamics and the design of effective vector control strategies for this region. | 2013 | 23497535 |
entomological monitoring and evaluation: diverse transmission settings of icemr projects will require local and regional malaria elimination strategies. | the unprecedented global efforts for malaria elimination in the past decade have resulted in altered vectorial systems, vector behaviors, and bionomics. these changes combined with increasingly evident heterogeneities in malaria transmission require innovative vector control strategies in addition to the established practices of long-lasting insecticidal nets and indoor residual spraying. integrated vector management will require focal and tailored vector control to achieve malaria elimination. ... | 2015 | 26259942 |
molecular comparison of topotypic specimens confirms anopheles (nyssorhynchus) dunhami causey (diptera: culicidae) in the colombian amazon. | the presence of anopheles (nyssorhynchus) dunhami causey in colombia (department of amazonas) is confirmed for the first time through direct comparison of mtdna cytochrome c oxidase i (coi) barcodes and nuclear rdna second internal transcribed spacer (its2) sequences with topotypic specimens of an. dunhami from tefé, brazil. an. dunhami was identified through retrospective correlation of dna sequences following misidentification as anopheles nuneztovari s.l. using available morphological keys fo ... | 0 | 21120360 |
evidence for temporal population replacement and the signature of ecological adaptation in a major neotropical malaria vector in amazonian peru. | the major neotropical malaria vector, anopheles darlingi, was reintroduced into the iquitos, loreto, peru area during the early 1990s, where it displaced other anophelines and caused a major malaria epidemic. since then, case numbers in loreto have fluctuated, but annual increases have been reported since 2012. | 2015 | 26415942 |
comparison of transmission parameters between anopheles argyritarsis and anopheles pseudopunctipennis in two ecologically different localities of bolivia. | anopheles (anopheles) pseudopunctipennis is a recognized malaria vector in the slopes of the andes of bolivia. there, other species might be involved in malaria transmission and one candidate could be anopheles argyritarsis. although it is generally admitted that this species is not a malaria vector in the neotropical region, its potential role in transmission is still controversial and this situation has to be cleared, at least for bolivia. comparing the vectorial efficiency of an. pseudopuncti ... | 2013 | 23941216 |
implications for changes in anopheles darlingi biting behaviour in three communities in the peri-iquitos region of amazonian peru. | malaria transmission in the peri-iquitos region of amazonian peru has been designated as seasonal and hypo-endemic with recently described hyper-endemic hotspots. despite relatively recent distribution of long-lasting insecticidal bed nets (llins), malaria in amazonian peru persists and increased substantially in 2014 compared to previous years. anopheles darlingi, identified as the main malaria vector, is known for its variable behaviour depending on locality and environment. | 2015 | 26223450 |
population dynamics, structure and behavior of anopheles darlingi in a rural settlement in the amazon rainforest of acre, brazil. | anopheles darlingi is the major vector of malaria in south america, and its behavior and distribution has epidemiological importance to biomedical research. in brazil, an. darlingi is found in the northern area of the amazon basin, where 99.5% of the disease is reported. | 2011 | 21702964 |
molecular taxonomy of anopheles (nyssorhynchus) benarrochi (diptera: culicidae) and malaria epidemiology in southern amazonian peru. | anopheline specimens were collected in 2011 by human landing catch, shannon and cdc traps from the malaria endemic localities of santa rosa and san pedro in madre de dios department, peru. most specimens were either anopheles (nyssorhynchus) benarrochi b or an. (nys.) rangeli, confirmed by polymerase chain reaction-restriction fragment length polymorphism-internal transcribed spacer 2 (pcr-rflp-its2) and, for selected individuals, its2 sequences. a few specimens from lupuna, loreto department, n ... | 2012 | 23243107 |
de novo biosynthesis of juvenile hormone iii and i by the accessory glands of the male mosquito. | the role of the male accessory glands (mag) in reproduction was investigated in the mosquito aedes aegypti. mag incubated with [14c]acetate synthesized radioactively labeled jh iii, jh iii bisepoxide and methyl farnesoate. mag incubated with l-[methyl-3h]methionine synthesized [3h]jh iii and a molecule that chromatographed on hplc with jh i. analysis of mag and whole males extract by glass capillary combined gas-chromatography-selected ion monitoring mass spectrometry identified jh iii and i as ... | 1994 | 8205141 |
anthropophilic anopheles species composition and malaria in tierradentro, córdoba, colombia. | malaria is still a primary health problem in colombia. the locality of tierradentro is situated in the municipality of montelíbano, córdoba, in the northwest of colombia, and has one of the highest annual parasite index of malaria nationwide. however, the vectors involved in malaria transmission in this locality have not yet been identified. in this study, the local anthropophilic anopheles composition and natural infectivity with plasmodium were investigated. in august 2009, 927 female anophele ... | 2014 | 24863977 |
geometric morphometrics for the taxonomy of 11 species of anopheles (nyssorhynchus) mosquitoes. | the subgenus anopheles (nyssorhynchus) (diptera: culicidae) includes the primary vectors of plasmodium spp. in colombia. most adult females of this subgenus are difficult to identify in the field using the available keys. with the objective of further investigating the discriminatory power of modern morphometrics, both landmark-based and outline-based approaches were explored using the wing venation geometry of 11 anopheles (nyssorhynchus) species. wing shape was able to separate the closest spe ... | 2015 | 25393150 |
genetic differentiation and diagnostic loci of anopheles nuneztovari, an. trinkae, and an. rangeli (diptera: culicidae). | samples of anopheles rangeli gabaldon, cova garcia & lopez, an. trinkae causey, and an. nuneztovari gabaldon from venezuela, ecuador, brazil, and bolivia were analyzed for genetic variability at 24 enzyme loci. estimates of genetic variability for an. rangeli and an. trinkae from ecuador and for an. nuneztovari in venezuela had the following ranges: 46-58% polymorphic loci, 1.7-2.0 (sem = 0.1-0.3) mean number of alleles per locus, and 0.069-0.113 (sem = 0.03-0.04) expected mean heterozygosity. g ... | 1995 | 7473622 |
mitochondrial dna variation within and between two species of neotropical anopheline mosquitoes (diptera:culicidae). | we analyzed variation in mitochondrial dna (mtdna) of two neotropical mosquitoes, anopheles rangeli (n = 181) and a. trinkae (n = 45), with very different distribution patterns in latin america, to assess species boundaries for these putative sister taxa and to examine population genetic structure. phylogenetic analyses revealed (1) support for the monophyletic origin of each species; (2) diagnostic restriction site differences between the species; (3) geographic partitioning of haplotypes by co ... | 2008 | 9099005 |