| use of modified nucleotides and uracil-dna glycosylase (ung) for the control of contamination in the pcr-based amplification of rna. | the inadvertent carryover of amplified fragments of nucleic acids (amplicons) is a potential source of contamination in the polymerase chain reaction. recently, a method has been developed to generate amplicons with deoxyuracil triphosphate (dutp) and to specifically hydrolyze these amplicons with uracil-dna glycosylase (ung) following the completion of the assay. we evaluated this system for the specific amplification of rna from coxsackievirus a3 and b3. we found that rna from both viruses cou ... | 1992 | 1406734 |
| spect in focal enterovirus encephalitis: evidence for local cerebral vasculitis. | we report a 4-year-old, left-handed male with focal coxsackievirus a3 encephalitis who presented with seizures and acquired aphasia. electroencephalography exhibited focal spike discharges over the right frontal regions, but cranial magnetic resonance imaging did not reveal any structural abnormalities. however, brain single-photon emission computed tomography performed during the acute phase disclosed focal hypoperfusion in the right frontal lobe, consistent with decreased regional cerebral blo ... | 2000 | 11118800 |
| epidemics of viral meningitis caused by echovirus 6 and 30 in korea in 2008. | enteroviruses (evs) are the leading cause of aseptic meningitis, which is the most frequent central nervous system infection worldwide. we aimed to characterize the evs involved in an aseptic meningitis outbreak in korea in 2008. in korea, echovirus type 30 (e30) and e6 have been associated with outbreaks and frequent meningitis. | 2012 | 22336050 |