Publications

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interaction between coccidia and nematodirus battus in lambs on pasture.five thousand oocysts of each of two species of coccidia, eimeria crandallis and e ovinoidalis or 30,000 infective larvae of nematodirus battus, given as single infections to three- to five-week-old lambs, caused only transient diarrhoea and had no effect on growth. lambs infected first with coccidia and two weeks later with n battus suffered severe diarrhoea, weight loss and some deaths. simultaneous administration of the coccidia and the nematodes increased the clinical severity of the syndrom ...19892756634
demonstration of antibodies to eimeria species in lambs by an enzyme-linked immunosorbent assay.soluble extracts were prepared from sporulated oocysts, unsporulated oocysts and merozoites of eimeria crandallis, e faurei and e ovinoidalis. they were assessed for antigenicity and specificity by elisa using rabbit antisera to sporulated oocysts or merozoites. antibody levels were examined in sera from colostrum-deprived coccidia-free lambs, conventionally reared lambs and lambs which had received experimental infections. maternal antibody was demonstrated in colostrum and in serum taken at 24 ...19873823625
pathogenicity of the coccidium eimeria crandallis in laboratory lambs.ten colostrum-deprived, coccidia-free lambs were reared artificially in indoor cages. at 4 weeks of age they were allocated to 5 groups of 2: groups 1-3 received 10(4), 10(5) and 10(6) sporulated oocysts of eimeria crandallis/lamb respectively, each as a single inoculum. group 4 lambs received daily doses increasing over a 4-week period. groups 1-3 showed diarrhoea and weight loss from about day 14, followed by slow recovery from about day 30. the diarrhoea was accompanied by a wave of oocyst ou ...19854034246
development of eimeria crandallis honess from sheep in cultured cells. 19725032227
observations on the epidemiology of coccidial infections in sheep under varying conditions of intensive husbandry including chemoprophylaxis with monensin.coccidiosis occurs sporadically in lambs at about 6 weeks of age when oocyst output is very high in healthy as well as in diseased lambs. these experiments were designed to throw light on the source of infection and to correlate oocyst output in lambs and ewes with performance in lambs. in two experiments, one indoors on deep litter the other in outdoor paddocks, oocyst output (of different coccidial species), body weight and clinical state of lambs were recorded weekly. oocyst output in ewes wa ...19836657301
immunisation of lambs against coccidiosis.two groups of twin lambs, kept with their dams at pasture, were given 10,000 oocysts of eimeria crandallis and 10,000 oocysts of e ovinoidalis either at birth only, or on four occasions at weekly intervals. a further group received 1000 oocysts of each species three times a week in a 'trickle infection' from birth to 21 days of age. all these lambs, together with a susceptible control group were challenged with 100,000 oocysts of each species at 28 days of age. a fifth group received no inoculat ...19938430481
output of coccidial oocysts (particularly eimeria crandallis) by naturally-infected lambs: daily and hourly patterns and clinical significance. 19873319488
observations on the endogenous stages of eimeria crandallis in domestic lambs (ovis aries).lambs reared coccidia-free were inoculated orally with various numbers of sporulated oocysts of e. crandallis and were killed between 1 and 22 days after inoculation; tissues were examined histologically. sporozoites were seen 1, 2 and 3 days after inoculation (dai) in crypt epithelial cells in the mid-jejunum. infected cells migrated into the lamina propria where the parasite within them developed into a first-generation meront containing about 250,000 merozoites at 10 dai. a second generation ...19892534531
ovine coccidiosis: the pathology of eimeria crandallis infection.doses of sporulated oocysts of eimeria crandallis ranging from 50 to 300,000,000 were given to 27 housed lambs aged between 4 and 12 weeks that had been reared coccidia-free. lambs were killed between 1 and 22 days after inoculation and their tissues examined histologically. clinical effects were very variable and not closely related to inoculating dose. some lambs showed intermittent diarrhoea, sometimes watery and sometimes containing muco-fibrinous material, either in the form of intestinal c ...19902276861
longitudinal prevalence, oocyst shedding and molecular characterisation of eimeria species in sheep across four states in australia.the prevalence of eimeria in sheep in australia has not been well described, therefore a quantitative pcr (qpcr) was developed, validated and used to study the prevalence and oocyst concentration in lamb faecal samples at three sampling periods (weaning, post-weaning and pre-slaughter) from eight farms across south australia, new south wales, victoria and western australia. a total of 3412 faecal samples were collected from approximately 1182 lambs across the 4 states and screened for the presen ...201425033746
diversity and seasonal occurrence of eimeria species in a mixed flock of communally reared sheep and goats in mafikeng in the north west province, south africa.diversity and seasonal occurrence of coccidia in a communally reared mixed flock of sheep and goats at mafikeng, north west province, south africa, was determined between march 2008 and february 2009. faecal specimens were collected directly from the rectum of the animals and the number of oocysts per gram of faeces (opg) determined. the mean monthly opg for goats was significantly higher than that for sheep. higher oocyst counts were observed during the hot, rainy season than during the cold, d ...201021247039
dose-response effects of diclazuril against pathogenic species of ovine coccidia and the development of protective immunity.twin lambs at pasture with their ewes, were divided into seven groups of 10 lambs. one group of 10 lambs served as a non-infected, untreated control. five groups of 10 lambs were infected with 10,000 oocysts of eimeria crandallis and 10,000 oocysts of eimeria ovinoidalis when they were 3 weeks old (day 21 of the study). this produced a good level of infection with high oocysts production and diarrhoea in the lambs. fourteen days after the primary, artificial challenge (day 35) four of these grou ...201021232870
study of the comparative efficacy of toltrazuril and diclazuril against ovine coccidiosis in housed lambs.a blinded, controlled and randomised field study was conducted on a sheep farm with a known history of coccidiosis and a high prevalence mainly of the pathogenic coccidium eimeria ovinoidalis. the efficacy of treatment with toltrazuril (baycox 5% suspension) against natural infections with eimeria crandallis and/or eimeria ovinoidalis in housed lambs was investigated in comparison with diclazuril and untreated controls. both drugs were administered either metaphylactically (i.e., in the prepaten ...200919575235
epidemiology of eimeria infections in an austrian milking sheep flock and control with diclazuril.in a flock of milking sheep from styria (austria), the dynamics of eimeria oocyst excretion was monitored in the lambing season 2003-2004 and a treatment trial with 1 mg/kg diclazuril (single dose on day 0 of study) was conducted in two groups of early weaned lambs. adult animals (n = 30 ewes, 30 yearlings) excreted oocysts of different species (weekly prevalences from week -7 ante partum to week 5 post-partum: ewes 20-60%, yearlings 38-73%) in low intensities (< or = 6000 oocyst per gram of fae ...200515817196
histopathological observations on the activity of diclazuril (vecoxan) against the endogenous stages of eimeria crandallis in sheep.doses of sporulated oocysts of eimeria crandallis were administered to 60 housed lambs aged 3-4 weeks that had been raised coccidia-free. thirty of the lambs were medicated with diclazuril at intervals over a 20-day period post-infection with the remaining lambs serving as untreated controls. lambs were euthanased between 5 and 22 days post-infection (dpi) and sections of the small intestine and caecum examined histologically. untreated lambs showed loss of surface epithelial cells and villous a ...200314580801
a descriptive epidemiological study of coccidiosis in early lambing housed flocks.infection with eimeria sp. was studied in 135 lambs born to 79 ewes in four early lambing housed flocks. in three of these flocks two different coccidiostats (monensin and decoquinate) were administered in the feed. cohort lambs were examined clinically and a rectal faeces sample taken once a week. samples with more than 300 oocyts per gram were speciated by morphology. nine species were identified and eimeria crandallis was the most prevalent. one of the flocks developed clinical coccidiosis be ...19947839559
development of dna probes for the ovine eimeria species e. crandallis and e. ovinoidalis.as an alternative to morphological speciation, dna probes have been developed to identify eimeria crandallis and e. ovinoidalis, the species associated with ovine coccidiosis. coccidia-free lambs were infected with pure cultures of e. crandallis and e. ovinoidalis. oocysts separated from faeces were sporulated and treated to release sporozoites from which dna was purified. repetitive dna sequences were identified by hybridisation and cloned. the specificity of selected clones was tested by hybri ...19957770428
molecular typing of eimeria ahsata and e. crandallis isolated from slaughterhouse wastewater. 201627303617
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