| the isolation and identification of several trichothecene mycotoxins from fusarium heterosporum. | an isolate of fusarium heterosporum nees ex fr. that parasitizes the honeydew and immature sclerotia of claviceps paspali was shown to be highly toxigenic to day-old chickens. the major toxicity of culture extracts of the fungus was due to the presence of several trichothecene mycotoxins. six trichothecenes were isolated and chemically identified by spectroscopic methods as t-2 toxin (1), ht-2 toxin (2), t-2 tetraol (6), 3alpha,4beta,dihydroxy-15-acetoxy-8alpha-(3-hydroxy-3-methylbutyryloxy)-12, ... | 1981 | 7264681 |
| moths that vector a plant pathogen also transport endophytic fungi and mycoparasitic antagonists. | claviceps paspali, a common fungal pathogen of paspalum grasses, attracts moth vectors by producing sugary exudates in the grass florets it infects. these exudates also support mycoparasitic fusarium species that may negatively influence c. paspali fitness. we examined the potential for moths on which c. paspali depends to also transmit mycoparasitic fusarium and fungal endophytes, which inhabit asymptomatic plant tissue and may influence host susceptibility to pathogens. we quantified infection ... | 2008 | 18491176 |
| c-terminal peptide of fusarium heterosporum lipase is necessary for its increasing thermostability. | saccharomyces cerevisiae bearing a lipase cdna from fusarium heterosporum produced two lipases, a and b. lipase b was significantly more stable to temperature than lipase a, but their optimum temperatures were similar. lipase b was composed of one polypeptide (301 amino acids), and lipase a was composed of two polypeptides (275 and 26 amino acids) generated by the cleavage between arg275 and asp276 with a trypsin-like protease. it was suggested that the c-terminal peptide (26 amino acids) tighte ... | 1998 | 9832617 |
| human toxicosis caused by moldy rice contaminated with fusarium and t-2 toxin. | this is the first report on human toxicosis in china caused by moldy rice contaminated with fusarium and t-2 toxin due to heavy rainfall during rice harvest season. one hundred and sixty-five persons ate the moldy rice and ninety-seven persons fall ill of food poisoning. the incidence was 58.8% and latent period was 10-30 min. the chief symptoms were nausea, dizziness, vomiting, chills, abdominal distension, abdominal pain, thoracic stuffiness and diarrhea. the fungi isolated from the moldy rice ... | 1993 | 8476534 |
| cloning and nucleotide sequence of cdna encoding a lipase from fusarium heterosporum. | fusarium heterosporum produces a solvent-tolerant lipase. a 1.3-kbp lipase cdna was isolated from the cdna library by colony hybridization with an oligonucleotide probe corresponding to the n-terminal amino acid sequence. nucleotide sequence analysis showed an open reading frame of 999 bp, and the deduced amino acid sequence contained the n-terminal sequence determined by edman degradation and the consensus pentapeptide (-gly-x-ser-x-gly-), which is conserved in lipase, esterase, and serine prot ... | 1994 | 7852271 |
| [food poisoning caused by mouldy rice contaminated with fusarium and t-2 toxin]. | an epidemic of food poisoning in human beings occurred in tongxiang county, zhejiang province after eating mouldy rice contaminated with fusarium and toxins. continuous rainfall took place during the rice harvest season. the number of patients poisoned was 97, the rate of incidence was 58.8%. the incubation period was 10 to 30 minutes. the chief symptoms were nausea, dizziness, vomiting, chills, abdominal distension, abdominal pain and diarrhea. the fungi isolated from the mouldy rice were predo ... | 1992 | 1298606 |
| evaluation of certain food additives and contaminants. eightieth report of the joint fao/who expert committee on food additives. | this report represents the conclusions of a joint fao/who expert committee convened to evaluate the safety of various food additives and contaminants and to prepare specifications for identity and purity. the first part of the report contains a brief description of general considerations addressed at the meeting, including updates on matters of interest to the work of the committee. a summary follows of the committee's evaluations of technical, toxicological and/or dietary exposure data for seve ... | 2016 | 27514183 |
| simultaneous conversion of free fatty acids and triglycerides to biodiesel by immobilized aspergillus oryzae expressing fusarium heterosporum lipase. | the presence of high levels of free fatty acids (ffa) in oil is a barrier to one-step biodiesel production. undesirable soaps are formed during conventional chemical methods, and enzyme deactivation occurs when enzymatic methods are used. this work investigates an efficient technique to simultaneously convert a mixture of free fatty acids and triglycerides (tag). a partial soybean hydrolysate containing 73.04% free fatty acids and 24.81% triglycerides was used as a substrate for the enzymatic pr ... | 2017 | 27860394 |
| establishment of the inducible tet-on system for the activation of the silent trichosetin gene cluster in fusarium fujikuroi. | the pks-nrps-derived tetramic acid equisetin and its n-desmethyl derivative trichosetin exhibit remarkable biological activities against a variety of organisms, including plants and bacteria, e.g., staphylococcus aureus. the equisetin biosynthetic gene cluster was first described in fusarium heterosporum, a species distantly related to the notorious rice pathogen fusarium fujikuroi. here we present the activation and characterization of a homologous, but silent, gene cluster in f. fujikuroi. bio ... | 2017 | 28379186 |
| an integrative process model of enzymatic biodiesel production through ethanol fermentation of brown rice followed by lipase-catalyzed ethanolysis in a water-containing system. | we attempted to integrate lipase-catalyzed ethanolysis into fermentative bioethanol production. to produce bioethanol, ethanol fermentation from brown rice was conducted using a tetraploid saccharomyces cerevisiae expressing α-amylase and glucoamylase. the resultant ethanol was distilled and separated into three fractions with different concentrations of water and fusel alcohols. in ethanolysis using the first fraction with 89.3% ethanol, a recombinant aspergillus oryzae whole-cell biocatalyst e ... | 2012 | 23273281 |
| development of an aspergillus oryzae whole-cell biocatalyst coexpressing triglyceride and partial glyceride lipases for biodiesel production. | an aspergillus oryzae whole-cell biocatalyst which coexpresses fusarium heterosporum lipase (fhl) and mono- and di-acylglycerol lipase b (mdlb) in the same cell has been developed to improve biodiesel production. by screening a number of transformants, the best strain was obtained when fhl gene was integrated into a. oryzae chromosome using sc selection marker while mdlb was integrated using niad selection marker. the reaction system using the lipase-coexpressing whole-cells was found to be supe ... | 2011 | 21507622 |
| highly efficient biodiesel production by a whole-cell biocatalyst employing a system with high lipase expression in aspergillus oryzae. | in the present study, a system with high lipase expression in aspergillus oryzae was developed using an improved enolase promoter (p-enoa124) and the 5' untranslated region of a heat-shock protein (hsp-utr). p-enoa142 enhanced the transcriptional level of a heterologous lipase gene and hsp-utr improved its translational efficiency. fusarium heterosporum lipase (fhl) was inserted into a psensu-fhl expression vector harboring p-enoa142 and hsp-utr and was transformed into an a. oryzae ns4 strain. ... | 2011 | 21380514 |
| preparation and comparative characterization of immobilized aspergillus oryzae expressing fusarium heterosporum lipase for enzymatic biodiesel production. | in this paper, we provide the first report of utilizing recombinant fungal whole cells in enzymatic biodiesel production. aspergillus oryzae, transformed with a heterologous lipase-encoding gene from fusarium heterosporum, produced fully processed and active forms of recombinant f. heterosporum lipase (fhl). cell immobilization within porous biomass support particles enabled the convenient usage of fhl-producing a. oryzae as a whole-cell biocatalyst for lipase-catalyzed methanolysis. the additio ... | 2008 | 18795281 |
| [experimental study of fast diagnosis of mycotic keratitis using molecular biology technical]. | to establish a method for fast diagnosis of mycotic keratitis using multi-pcr system. | 2007 | 17605910 |
| equisetin biosynthesis in fusarium heterosporum. | an iterative polyketide synthase-peptide synthetase hybrid assembles the hiv-1 integrase inhibitory tetramic acid, equisetin, in the filamentous fungus fusarium heterosporum. | 2004 | 15724180 |
| cloning and expression analysis of nhl1, a gene encoding an extracellular lipase from the fungal pea pathogen nectria haematococca mp vi (fusarium solani f. sp. pisi) that is expressed in planta. | the filamentous fungus nectria haematococca (anamorph fusarium solani f. sp. pisi) resides in soil, and attacks pea seedlings in the area of the underground epicotyl and upper tap root, causing foot rot disease. we detected lipase activity during in vitro growth of n. haematococca. subsequently, a lipase gene was cloned and functionally characterised by heterologous expression in saccharomyces cerevisiae. the full-length cdna of 1152 bp was cloned using a 3' race-pcr approach coupled with cdna l ... | 2001 | 11361331 |
| mangicols: structures and biosynthesis of a new class of sesterterpene polyols from a marine fungus of the genus fusarium. | a marine fungal isolate, tentatively identified as fusarium heterosporum, has been found to produce a series of structurally novel sesterterpene polyols, the mangicols a-g (4-10). the structures of the new compounds, including the stereochemistry of mangicol a, were assigned by interpretation of spectral data derived from both natural products and synthetic derivatives. the mangicols, which possess unprecedented spirotricyclic skeletal components, show only weak to modest cytotoxicities toward a ... | 2000 | 10956462 |
| isolation and characterization of novel human immunodeficiency virus integrase inhibitors from fungal metabolites. | we have identified a series of novel inhibitors of human immunodeficiency virus type 1 (hiv-1) integrase by randomly screening natural product extracts using an in vitro biochemical assay designed to identify inhibitors of integrase-catalysed strand transfer. equisetin recovered from the fungus fusarium heterosporum and a novel enantiomeric homologue of equisetin from phoma sp. were isolated as inhibitors of hiv-1 integrase in vitro. two additional analogues, a novel decalin derivative, integric ... | 1999 | 10335400 |
| amino acid residues contributing to stabilization of fusarium heterosporum lipase. | fusarium heterosporum lipase is composed of an n-terminal large peptide of 275 amino acids and a c-terminal peptide of 26 amino acids. the thermostability of the lipase was remarkably decreased by cleavage of the c-terminal peptide. hence, we attempted to specify the amino acids in the c-terminal peptide that are responsible for the stabilization of the lipase. replacement of asp293 with ala, asn, and lys caused a significant decrease in thermostability, but its mutation to glu did not decrease ... | 2000 | 16232775 |
| two related pyrrolidinedione synthetase loci in fusarium heterosporum atcc 74349 produce divergent metabolites. | equisetin synthetase (eqis), from the filamentous fungus fusarium heterosporum atcc 74349, was initially assigned on the basis of genetic knockout and expression analysis. increasing inconsistencies in experimental results led us to question this assignment. here, we sequenced the f. heterosporum genome, revealing two hybrid polyketide-peptide proteins that were candidates for the equisetin synthetase. the surrounding genes in both clusters had the needed auxiliary genes that might be responsibl ... | 2013 | 23614392 |
| native promoter strategy for high-yielding synthesis and engineering of fungal secondary metabolites. | strategies are needed for the robust production of cryptic, silenced, or engineered secondary metabolites in fungi. the filamentous fungus fusarium heterosporum natively synthesizes the polyketide equisetin at >2 g l(-1) in a controllable manner. we hypothesized that this production level was achieved by regulatory elements in the equisetin pathway, leading to the prediction that the same regulatory elements would be useful in producing other secondary metabolites. this was tested by using the n ... | 2015 | 25226362 |
| biosynthesis of the tetramic acids sch210971 and sch210972. | a biosynthetic pathway to fungal polyketide-nonribosomal peptide natural products, sch210971 (1a) and sch210972 (1b) from hapsidospora irregularis, was characterized by reconstitution and heterologous expression in fusarium heterosporum. using genetic, biochemical, and feeding experiments, we show that the incorporated amino acid 4-hydroxyl-4-methyl glutamate (hmg) is synthesized by an aldolase, probably using pyruvate as the precursor. | 2015 | 25885659 |
| evaluation of filamentous fungi isolated from petals of bean and rapeseed for suppression of white mold. | the influence of filamentous fungi isolated from petals of bean and rapeseed on white mold caused by sclerotinia sclerotiorum was evaluated. in laboratory trials, macerates of agar plugs containing hyphal fragments of the pathogen in combination with individual fungi were applied onto celery petioles, and subsequent lesion diameters were recorded. the efficacy of 10 fungi exhibiting a spectrum of lesion suppression on celery was correlated with the efficacy of the same fungi in growth-room (r = ... | 1992 | 1521186 |