[helping factors of the occlusion and oppening mechanisms of gastric cardiac region in opossum. comparative study to the man (author's transl)].the morphological factors that should work to help the cardial occlusion (esophageal and gastric mucosal folds) and are arranged to cooperate for the cardial oppening (smooth muscle fibres of the longitudinal layer of esophagus and stomach) are described in opossum (didelphis albiventris), comparatively to the man. also are comparatively discussed the anatomical disposition of diaphragm, esophageal hiatus of diaphragm, gastrophrenic ligament and phrenosophagical membrane.1979543562
oncicola martini sp. n., and other archiacanthocephala of the chaco boreal, paraguay.archiacanthocephalans are reported from mammals of paraguay. oncicola martini sp. n. is described from felis geoffroyi. it is most similar to o. lamasi (freitas et costa 1964) comb. n., differing in having larger hooks, all except the smallest of which are barbed. other species recorded are oncicola oncicola from felis geoffroyi (new host record), o. paracampanulata from felis yagouaroundi, o. luehei from nasua nasua, oligacanthorhynchus microcephala from didelphis albiventris, and o. tortuosa f ...1977864568
sylvatic american trypanosomiasis in argentina. trypanosoma cruzi infection in mammals from the chaco forest in santiago del estero.trypanosoma cruzi infection in sylvatic mammals of the quebracho woods of the eastern part of santiago del estero province, argentina, was studied from october 1984 to december 1987. 301 mammals of 20 different species were caught. t. cruzi, characterized biologically and biochemically, was isolated by xenodiagnosis from 23 of 72 (32%) didelphis albiventris opposums, 2/36 (5.5%) conepatus chinga skunks, and one ferret (galictis cuja). 53 opossum refuges were located and triatomine bugs were foun ...19921566301
the importance of the opossum (didelphis albiventris) as a reservoir for trypanosoma cruzi in bambuí, minas gerais a survey realized on the sylvatic and peridomestic environments at bambuí county, minas gerais state, 44 (37.9%) out of 116 opossums (didelphis albiventris) captured were found to be naturally infected with trypanosoma cruzi. one hundred and forty three parasite samples were obtained from 43 infected opossums using simultaneously hemoculture, xenodiagnosis (triatoma infestans, panstrongylus megistus and rhodnius neglectus) and examination of anal glands contents. the parasite samples were cha ...19911842405
effects of hydrocortisone acetate treatment on the small intestine of the lactent marsupial didelphis albiventris.when hydrocortisone acetate was administered subcutaneously to the lactent brazilian white belly opossum, it induced precocious intestinal maturation. histological features of the small intestine were reminiscent of those seen in human protein-caloric malnutrition. there were also indications that the glucocorticoid acted as an inducer of the differentiation of paneth, goblet and granulomucous cells within the small intestinal epithelium. in the lactent opossum the small intestine is lined by an ...19912064018
[natural infection of the anal glands of the opossum (didelphis albiventris) by trypanosoma cruzi in the municipality of bambuí--mg, brazil].out of 87 opossums, didelphis albiventris, captured in the bambuí area (minas gerais state), 32 (36.7%) were found infected by trypanosoma cruzi; the rates varied according to whether the specimens originated from sylvan, rural peridomiciliar or urban surroundings, being 34.9, 81.8 and 7.7 respectively. from 20 of the infected opossums the anal glands were repeatedly examined and found positive in only one (5%) specimen (ga 9), with 7 positive examinations out of 17 performed through an 18-month ...19892108308
experimental infection of the opossum didelphis albiventris (marsupialia, didelphidae) with leishmania donovani. 19883249558
cytogenetic analysis of some brazilian marsupials (didelphidae: marsupialia).three species of marsupials from the amazon region (marmosa cinerea, caluromys lanatus, and didelphis marsupialis) and two from the region of são paulo (didelphis marsupialis and didelphis albiventris) were studied. the g-banding pattern of the species with 2n = 14 (m. cinerea and c. lanatus) was very similar, as well as the pattern of g-bands in the species with 22 chromosomes (didelphis). all of the autosomes of m. cinerea and d. albiventris have centromeric c-bands and the y chromosome is tot ...19863697826
spatial learning in south american opossums and armadillos.this experiment compares spatial learning in the south american opossum (didelphis albiventris) and armadillo (chaetophractus villosus) in a y-maze. ss learned to turn to one arm of the maze for food reinforcement. to earn further reinforcements, they had to return to the start-box (after consuming the food) where they were restrained for a fixed intertrial interval. the number of entries to the unbaited arm en route to the goal-box (ri errors), and in the way back to the start-box (rf errors) w ...19846470686
natural infection of the opossum didelphis albiventris (marsupialia, didelphidae) with leishmania donovani, in opossum, didelphis albiventris, from jacobina, bahia state, was found naturally infected with leishmania donovani, being the first non-canid wild mammal to be detected with the agent of kala-azar in the new world.19846533423
histochemical and ultrastructural studies of mast cells in the intestinal mucosa and skin of the opossum didelphis albiventris.the mast cells located in the intestinal mucosa of a non-eutherian mammal were studied in comparison with those in the skin of the ear. in the intestinal mucosa, the mast cells exhibited a more variable shape, larger cytoplasmic granules and greater sensitivity to fixatives regarding their staining towards toluidine blue. ultrastructurally, these granules were more heterogeneous but lacked the crystalline content found in granules of skin mast cells; lipid body-like organelles were found only in ...19947515867
[possibility of spontaneous cure in opossums (didelphis albiventris) naturally infected by trypanosoma cruzi]. 19947569616
animal reservoirs for trypanosoma cruzi infection in an endemic area in paraguay.animal reservoirs for trypanosoma cruzi infection were investigated in 5 communities in the department of san pedro, currently one of paraguay's most highly endemic areas. a total of 112 domestic animals (37 cattle, 2 horses, 1 ass, 20 pigs, 44 dogs and 8 cats) and 4 wild animals (1 white-eared opossum, 2 yellow armadillos and 1 common long-nosed armadillo) were examined for blood. although no trypomastigotes were found by 2 direct observation methods, the microhaematocrit and giemsa stained thi ...19948075219
immunocytochemical study of peyer's patches follicular-associated epithelium in the marsupial, didelphis albiventris.the lack of probes defining leukocyte subpopulations has restricted ontogenetic studies of the opossum gut. we report for the first time the organization of the gut cellular immune components using species cross-reactive antibodies. mouse monoclonal antibodies against human hla-dr were used together with immunocytochemistry to demonstrate mhc class ii-like antigens in the opossum peyer's patches (pp). positive staining was obtained in the m cell and enterocytes comprising the follicular-associat ...19938299852
interaction between didelphis albiventris and triatoma infestans in relation to trypanosoma cruzi transmission.this paper attempts to prove if a high trypanosoma cruzi prevalence of opossums might be reached with few potential infective contacts. one non-infected didelphis albiventris to t. cruzi and 10 infected nymphs of triatoma infestans were left together during 23 hr in a device that simulated a natural opossum burrow. twenty-six replicates were performed using marsupials and triatomines only once. potentially infective contacts occurred in all the trials. from the 26 opossums used in trials, 54% di ...19958731363
search for trypanosoma cruzi in the anal glands of wild didelphis albiventris from santiago del estero, argentina. 19958731365
ecological interactions of visceral leishmaniasis in the state of bahia, brazil.the laboratory and field observations summarized in this paper on visceral leishmaniasis ecology in the state of bahia, brazil are based on the author's observations over the past 35 years in a number of state's foci, public health records and literature citations. the disease is endemic with epidemic outbreaks occurring every ten years and its geographical distribution is expanding rapidly in the last years. leishmania chagasi is the main ethiologic agent of the visceral leishmaniasis but le. a ...19969283643
characterization of trypanosoma cruzi strains isolated from chronic chagasic patients, triatomines and opossums naturally infected from the state of rio grande do sul, brazil.thirty-five trypanosoma cruzi strains were isolated from chronic chagasic patients, triatomines and opossums from different municipalities of the state of rio grande do sul. parasites were characterized by means of mice infectivity, enzyme electrophoresis and randomly amplified polymorphic dna (rapd) analysis. twenty-nine strains were isolated from chagasic patients, 4 from triatomines (2 from triatoma infestans and 2 from panstrongylus megistus) and 2 from opossums didelphis albiventris. thirty ...19979332599
[first evidence of trypanosoma rangeli in the southeast of brazil, an endemic region for chagas' disease].this short communication informs the discovery of trypanosoma rangeli for the first time at triângulo mineiro region, south-east of brazil, a highly endemic area of chagas' disease and also the natural infection of didelphis albiventris with the same trypanosome. both the findings were demonstrated through blood smears, xenodiagnosis, microhematocrit technics and pcr. the last one was realized in faeces and hemolymph of triatoma infestans utilizing as controls strains of t. rangeli from colombia ...19989477704
helminths of white-bellied opossum from brazil.helminths were collected from 22 specimens of the white-bellied opossum (didelphis albiventris) captured in the region of pampulha (belo horizonte, brazil), from may 1985 to march 1995. ten species of helminths were collected; these included rhopalias coronatus, brachylaema migrans, aspidodera raillieti, cruzia tentaculata, turgida turgida, gongylonema sp., viannaia hamata, travassostrongylus orloffi, trichuris didelphis and capillaria sp.199910231765
[contribution to knowledge of reservoirs of trypanosoma cruzi (chagas, 1909) in corrientes province, argentina].in order to identify trypanosoma cruzi reservoirs in transmission areas, 60 mammals in capital and san luis del palmar departments, corrientes, argentina were studied. primates, rodents, carnivores, marsupials and edentates were investigated, 40 of them living in captivity and 20 caught with traps in a rural area. the mammals were examined by xenodiagnosis and third or fourth instars nymphs of triatoma infestans starved for 2 weeks were used. the feces were microscopically observed (400x) for tr ...199910380566
isolation of sarcocystis falcatula from the south american opossum (didelphis albiventris) from argentina.sarcocystis sporocysts from the intestines of four opossums (didelphis albiventris) from argentina were identified as sarcocystis falcatula based on schizogonic stages and pathogenicity to budgerigars (melopsittacus undulatus). seven budgerigars fed sporocysts from the opossum feces died of acute sarcocystosis 8, 9, 11, 12, and 14 days after inoculation. schizonts and merozoites found in the lungs and other organs of the budgerigars were identified as s. falcatula based on structure and immunore ...199910536981
[prevalence of trypanosoma cruzi infection in opossum (didelphis albiventris) in santiago del estero, argentina].the opossum of the genus didelphis is one of the principal wild reservoirs of trypanosoma cruzi and is widely distributed in the western hemisphere. didelphis albiventris is the most common marsupial in amamá and trinidad, two communities in the province of santiago del estero, argentina. the d. albiventris population is replaced every year, and the opossum normally has two reproductive periods, one at the beginning of the spring and another at the beginning of the summer. the two litters are we ...199910659667
isolation of sarcocystis speeri dubey and lindsay, 1999 parasite from the south american opossum (didelphis albiventris) from argentina.sarcocystis sporocysts from the intestines of 2 opossums (didelphis albiventris) from argentina were fed to gamma-interferon knockout (ko) and nude mice. protozoal schizonts were seen in brain, liver, spleen, and adrenal glands of mice examined 33-64 days after feeding sporocysts. sarcocysts were seen in skeletal muscles of ko mice 34-71 days after feeding sporocysts. schizonts and sarcocysts were structurally similar to sarcocystis speeri dubey and lindsay, 1999 seen in mice fed sporocysts from ...200010701583
characterization of sarcocystis falcatula isolates from the argentinian opossum, didelphis albiventris.two isolates of sarcocystis falcatula were obtained from the lungs of budgerigars (melopsittacus undulatus) fed sporocysts from two naturally-infected south american opossums (didelphis albiventris). the two isolates were designated sf-1a and sf-2a. both isolates induced fatal infections in budgerigars. both isolates underwent schizogony in african green monkey kidney cells. the structure of schizonts in the lungs of budgerigars was more variable than that observed in cell culture. the two isola ...200010847342
experimental transmission of sarcocystis speeri dubey and lindsay, 1999 from the south american opossum (didelphis albiventris) to the north american opossum (didelphis virginiana).sarcocystis speeri dubey and lindsay, 1999 from the south american opossum didelphis albiventris was successfully transmitted to the north american opossum didelphis virginiana. sporocysts from a naturally infected d. albiventris from argentina were fed to 2 gamma-interferon knockout (ko) mice. the mice were killed 64 and 71 days after sporocyst feeding (daf). muscles containing sarcocysts from the ko mouse killed 71 daf were fed to a captive d. virginiana; this opossum shed sporocysts 11 days a ...200010864267
in vitro cultivation of schizonts of sarcocystis speeri dubey and lindsay, 1999.schizonts of sarcocystis speeri dubey and lindsay, 1999 were cultured in vitro in bovine monocyte and equine kidney cell cultures inoculated with infected tissues of nude and gamma-interferon knockout mice fed sporocysts from opossums, didelphis albiventris. at least 1 asexual cycle was completed in 3 days. in vitro-grown merozoites were structurally and antigenically distinct from those of sarcocystis neurona and sarcocystis falcatula. culture-derived merozoites of s. speeri were not infective ...200010958438
life cycle of ixodes (ixodes) loricatus (acari: ixodidae) under laboratory conditions.the life cycle of ixodes (ixodes) loricatus neumann, reared in the laboratory, is described. engorged females collected from opossums trapped in the states of minas gerais and são paulo, brazil, which were used to start the laboratory colonies, were designated as bmg and csp, respectively. larval and nymphal ticks from both colonies fed separately on rattus norvergicus berkenhout or calomys callosus rengger, whereas didelphis marsupialis l and didelphis albiventris lund were used as hosts for bm ...200011004783
a survey of hemoparasite infections in free-ranging mammals and reptiles in french guiana.blood smears of 1,353 free-ranging mammals (35 species) and 112 reptiles (31 species) from french guiana were examined for hemoparasites. parasites from 3 major groups were recorded: apicomplexa (including hemogregarines, piroplasms, and plasmodium spp.), trypanosomatidae, and filaroidea. fifty percent of the individuals (86% of the species) were infected by parasites from at least 1 group. hemogregarines, identified as hepatozoon sp., infected numerous snakes with high prevalences (30-100%); in ...200011128476
characterization of an unidentified sarcocystis falcatula-like parasite from the south american opossum, didelphis albiventris from unidentified isolate of a sarcocystis falcatula-like parasite was obtained from the lungs of budgerigars (melopsittacus undulatus) fed sporocysts from a naturally-infected south american opossum, didelphis albiventris from brazil. four captive budgerigars fed sporocysts from the opossum intestine died of acute sarcocystosis 8, 10, and 12 days after oral inoculation (dai); one budgerigar was killed 12 dai when it was lethargic. schizonts and merozoites found in the lungs of the budgerigars rea ...200011128705
a review of sarcocystis neurona and equine protozoal myeloencephalitis (epm).equine protozoal myeloencephalitis (epm) is a serious neurological disease of horses in the americas. the protozoan most commonly associated with epm is sarcocystis neurona. the complete life cycle of s. neurona is unknown, including its natural intermediate host that harbors its sarcocyst. opossums (didelphis virginiana, didelphis albiventris) are its definitive hosts. horses are considered its aberrant hosts because only schizonts and merozoites (no sarcocysts) are found in horses. epm-like di ...200111223193
[description of the external morphology of duboisiella proloba baer, 1983 (trematoda: strigeida) from didelphis albiventris in argentina, by scanning electronic microscope].the aim of this study was to report some external morphological features of duboisiella proloba baer, 1938. the parasites were collected during necropsy from the small intestine of didelphis albiventris hunted in its native environment near corrientes city, argentina. collected specimens of duboisiella proloba were observed by light microscopy in order to be classified. later the parasites were put into a 3% glutaraldehyde solution, dehydrated, dried by critical point, assembled, metallized, obs ...200011338969
probability of trypanosoma cruzi transmission by triatoma infestans (hemiptera: reduviidae) to the opossum didelphis albiventris (marsupialia: didelphidae).the probability of trypanosoma cruzi transmission to opossums by independent events of predation and fecal contamination during feeding ("biting") with positive triatoma infestans was estimated. negative female opossums were challenged for 23 hr with 10 infected third and fourth instars of t. infestans, and tests for positivity for t. cruzi by xenodiagnosis were performed at 30, 60, and 90 days. from these data, seven probability parameters were estimated by maximum likelihood, and likelihood ra ...200111508386
the marsupial didelphis albiventris is an improbable host of paracoccidioides brasiliensis in an endemic area of paracoccidioidomycosis in minas gerais, determine whether didelphis albiventris is naturally infected with paracoccidioides brasiliensis, 20 specimens of this mammal were studied by both direct cultivation of their viscera (spleen, liver and lungs) and by inoculation of swiss mice by the intraperitoneal route with a suspension of fragments of these viscera. no fungal growth or structures similar to this fungus were detected. probably d. albiventris is not frequently infected with p. brasiliensis.200111562699
sarcocystis lindsayi n. sp. (protozoa: sarcocystidae) from the south american opossum, didelphis albiventris from brazil.a new species, sarcocystis lindsayi n. sp., is proposed for a parasite resembling sarcocystis falcatula. it was obtained from the lungs and muscles of budgerigars (melopsittacus undulatus) fed sporocysts from a naturally-infected south american opossum, didelphis albiventris, from jaboticabal, brazil. sarcocysts of s. lindsayi n. sp. in budgerigars are microscopic, up to 600 microm long and up to 50 microm wide. the cyst wall is up to 2 microm thick. ultrastructurally, the sarcocyst wall consist ...200111596925
sarcocystis neurona infections in raccoons (procyon lotor): evidence for natural infection with sarcocysts, transmission of infection to opossums (didelphis virginiana), and experimental induction of neurologic disease in raccoons.equine protozoal myeloencephalitis (epm) is a serious neurologic disease of horses in the americas and sarcocystis neurona is the most common etiologic agent. the distribution of s. neurona infections follows the geographical distributions of its definitive hosts, opossums (didelphis virginiana, didelphis albiventris). recently, cats and skunks were reported as experimental and armadillos as natural intermediate hosts of s. neurona. in the present report, raccoons (procyon lotor) were identified ...200111698157
isolates of sarcocystis falcatula-like organisms from south american opossums didelphis marsupialis and didelphis albiventris from são paulo, brazil.isolates of sarcocystis falcatula-like organisms from south american opossums were characterized based on biological and morphological criteria. sporocysts from intestinal scrapings of 1 didelphis marsupialis and 8 didelphis albiventris from são paulo, brazil, were fed to captive budgerigars (melopsittacus undulatus). budgerigars fed sporocysts from all 9 isolates became ill and s. falcatula-like schizonts were identified in sections of their lungs by immunohistochemical staining. sarcocystis fa ...200111780836
search for borrelia sp. in ticks collected from potential reservoirs in an urban forest reserve in the state of mato grosso do sul, brazil: a short report.a total of 128 ticks of the genus amblyomma were recovered from 5 marsupials (didelphis albiventris) - with 4 recaptures - and 17 rodents (16 bolomys lasiurus and 1 rattus norvegicus) captured in an urban forest reserve in campo grande, state of mato grosso do sul, brazil. of the ticks collected, 95 (78.9%) were in larval form and 22 (21.1%) were nymphs; the only adult (0.8%) was identified as a. cajennense. viewed under dark-field microscopy in the fourth month after seeding, 9 cultures prepare ...200212219124
high prevalence of trypanosoma rangeli and trypanosoma cruzi in opossums and triatomids in a formerly-endemic area of chagas disease in southeast brazil trypanosoma rangeli has been detected in humans, sylvatic mammals and vectors in the amazon basin and in wild rodents in a southern state. here we report for the first time a high prevalence of t. rangeli in opossums and triatomids captured in peridomestic environments in a formerly-endemic area of chagas disease in southeast brazil. five molecular typing tools clearly indicate the presence of t. rangeli and trypanosoma cruzi in mammalian reservoirs and triatomids. twenty-one opossums ...200212443797
life-cycle and host specificity of amblyomma tigrinum (acari: ixodidae) under laboratory conditions.biological data of three generations of amblyomma tigrinum in the laboratory are reported and the suitability of different host species for immature ticks are compared. grouping the three generations, infestations by both the larval and nymphal stages were performed on chickens (gallus gallus), wistar rats (rattus norvegicus), rabbits (orytolagus cuniculus), wild mice (calomys callosus), dogs (canis familiaris) and opossums (didelphis albiventris). only dogs were used for infestations by adult t ...200212475081
[relationships between trypanosoma cruzi transmission cycles in the county of bambuí, minas gerais, brazil].this study examines recent relationships between domestic and sylvatic transmission cycles of t. cruzi in the county of bambuí, mg, brazil. in the late l930s, panstrongylus megistus was found in 75% of houses. subsequently, triatoma infestans became the predominant species, found in 20% of urban households and in more than 60% of periurban homes. with intense insecticide control campaigns between l956 and l969, t. infestans was eradicated from the county, transmission of chagas' disease to man w ...200314676933
histoplasma capsulatum isolated from didelphis albiventris (marsupialia: didelphidae) in the state of minas gerais, brazil.we report the isolation of histoplasma capsulatum from a culture of the viscera of didelphis albiventris, one of the marsupial species found in brazil. to our knowledge, this is the first report of the isolation of this fungus from this mammalian species. this finding confirms the ubiquitous presence of h. capsulatum in nature.200115496125
enzootic transmission of trypanosoma cruzi and t. rangeli in the federal district of brazil.the federal district of brazil (df) lies within the cerrado biome, where open shrubland (savannas) is interspersed with riverside gallery forests and permanent swamps (veredas). trypanosoma cruzi-infected native triatomines occur in the area, but the enzootic transmission of trypanosomatids remains poorly characterized. a parasitological survey involving sylvatic triatomines (166 rhodnius neglectus collected from mauritia flexuosa palms) and small mammals (98 marsupials and 70 rodents, totaling ...200415654478
trypanosoma cruzi infection in wild mammals of the national park 'serra da capivara' and its surroundings (piaui, brazil), an area endemic for chagas disease.we studied the prevalence of trypanosoma cruzi infection among eight species of wild small mammals (n=289) in an area where human cases of infection/disease have occurred. dogs (n=52) and goats (n=56) were also surveyed. the study was carried out inside a biological reserve, the national park 'serra da capivara' and its surroundings in piaui state, brazil. the marsupial didelphis albiventris and the caviomorph rodent trichomys apereoides were found to be the most important reservoirs in the stud ...200515780345
fibroblast growth factor 9: cloning and immunolocalisation during tooth development in didelphis albiventris.there are no reports in literature about functional roles of fibroblast growth factor 9 (fgf-9) in tooth development in animals with complete tooth pattern. the classical model for studying tooth development is the mouse, which has small number of teeth and distinctive incisor and molar patterns. the opossum didelphis albiventris with five upper and four lower incisors, one canine, three premolars, and four molars, on each side of the jaw, seems to be a convenient model to test results obtained ...200616188224
susceptibility of different triatomine species to trypanosoma rangeli experimental infection.trypanosoma rangeli is a kinetoplastid protozoan parasite that has been found in the majority of latin american countries, overlapping its distribution area with that of trypanosoma cruzi, the causative agent of chagas disease. this parasite shares the same reservoirs and vectors as t. cruzi. triatomines from genus rhodnius are considered the most susceptible hosts to infection. in this work, we report the susceptibility of different triatomine species (rhodnius neglectus, panstrongylus megistus ...200616584327
long-term reduction of trypanosoma cruzi infection in sylvatic mammals following deforestation and sustained vector surveillance in northwestern argentina.long-term variations in the dynamics and intensity of sylvatic transmission of trypanosoma cruzi were investigated around eight rural villages in the semiarid argentine chaco in 2002-2004 and compared to data collected locally in 1984-1991. of 501 wild mammals from 13 identified species examined by xenodiagnosis, only 3 (7.9%) of 38 didelphis albiventris opossums and 1 (1.1%) of 91 conepatus chinga skunks were infected by t. cruzi. the period prevalence in opossums was four-fold lower in 2002-20 ...200616839513
morphological study of tetratrichomonas didelphidis isolated from opossum lutreolina crassicaudata by scanning electron microscopy.tetratrichomonas didelphidis is a flagellate protozoan found in the intestine of opossums didelphis marsupialis, didelphis albiventris, and lutreolina crassicaudata. the isolate used in this study was from l. crassicaudata and it was cultivated in monoxenic culture with escherichia coli in diamond (tym) medium without maltose and with starch solution (trypticase-yeast extract-starch), ph 7.5 at 28 degrees c. scanning electron microscopy showed the fine morphological features of the trophozoites: ...200717252276
mapping of the distribution of trypanosoma cruzi infection among small wild mammals in a conservation unit and its surroundings (northeast-brazil).maps are a useful tool that permits correlation of landscapes with hotspots of parasite transmission. here, they were used as a tool for geovisualization to evaluate variables involved in the transmission of trypanosoma cruzi among small wild mammals in an area endemic for chagas disease, the "serra da capivara" national park (parna) and its surroundings in piauí state, northeast brazil. the implementation of a geographical information system (gis) allowed the observation that a previously noted ...200717307027
role of bmp-4 during tooth development in a model with complete dentition.there are no reports in literature about roles of bone morphogenetic protein 4 (bmp-4) in tooth development in mammals with complete dentition (with all dental groups). the classical model of study is the mouse, which has distinctive incisor and molar patterns. the opossum didelphis albiventris with five upper and four lower incisors, one canine, three premolars and four molars, on each side of the jaw, seems to be a convenient model for odontogenesis study. this investigation searched for simil ...200817803954
domiciliation of triatoma pseudomaculata (corrêa e espínola 1964) in the jequitinhonha valley, state of minas gerais.impact of the vector control program was evaluated eight years after implantation of epidemiological surveillance for chagas disease in berilo, a municipality in the jequitinhonha valley of the brazilian state of minas gerais. in all 5,242 domiciliary units (96% of the total) were inspected and 10 found to be infested by the triatomine bug triatoma pseudomaculata. triatomines were found associated with bats inside one house and in the peridomiciles of the other nine. none of the 111 triatoma pse ...200717876457
molecular detection of paracoccidioides brasiliensis in road-killed wild animals.paracoccidioides brasiliensis infections have been little studied in wild and/or domestic animals, which may represent an important indicator of the presence of the pathogen in nature. road-killed wild animals have been used for surveillance of vectors of zoonotic pathogens and may offer new opportunities for eco-epidemiological studies of paracoccidiodomycosis (pcm). the presence of p. brasiliensis infection was evaluated by nested-pcr in tissue samples collected from 19 road-killed animals; 3 ...200817885959
characterization of trypanosoma cruzi isolated from humans, vectors, and animal reservoirs following an outbreak of acute human chagas disease in santa catarina state, brazil.during march 2005, 24 cases of acute human chagas disease were detected in santa catarina state, southern brazil, all of them related to the ingestion of trypanosoma cruzi-contaminated sugar cane juice. following field studies allowed the isolation of 13 t. cruzi strains from humans, opossums (didelphis aurita and didelphis albiventris), and vectors (triatoma tibiamaculata). the isolated strains were characterized by multilocus enzyme electrophoresis (mlee) and analysis of the spliced-leader and ...200817889480
occurrence of gastrointestinal protozoa in didelphis albiventris (opossum) in the central region of rio grande do sul state.the aim of this study was to evaluate the parasitism by gastrointestinal protozoa in didelphis albiventris (opossum) in the central region of rio grande do sul state. fecal samples from six free living opossums were collected for research of parasites. samples were analyzed by the centrifugal-flotation method with zinc sulfate and parasites were identified microscopically based on (oo)cyst size and morphology. cysts of giardia sp. and oocysts of cryptosporidium sp. and eimeria sp. were observed ...200818035587
karyotype composition of some rodents and marsupials from chapada diamantina (bahia, brasil).the chapada diamantina (cd) is located in bahia state, between 11-14 degrees s and 41-43 degrees w, being part of the serra do espinhaço. the occurrence of different habitats and transition areas permits an interesting mammal fauna composition, with species from different biomes living in sympatry. species of didelphimorphia and rodentia are important members of mammal communities in almost all different habitats, and morphological and cytogenetic characters are important for a correct identific ...200718094834
habitat associations of small mammals in southern brazil and use of regurgitated pellets of birds of prey for inventorying a local fauna.we inventoried terrestrial small mammals in an agricultural area in southern brazil by using trapping and prey consumed by barn owls (tyto alba) and white-tailed kites (elanus leucurus). small mammals were trapped in three habitat types: corn fields, uncultivated fields ("capoeiras"), and native forest fragments. a total of 1,975 small mammal specimens were trapped, another 2,062 identified from the diet of barn owls, and 2,066 from the diet of white-tailed kites. both trapping and prey in the p ...200718278312
an outbreak of sarcocystosis in psittacines and a pigeon in a zoological collection in brazil.this report describes an outbreak of acute pulmonary sarcocystosis in different species of captive psittacines and in a luzon bleeding-heart pigeon (gallicolumba luzonica) in a zoological collection in brazil. a majority of the birds were found dead and had exhibited no previous clinical signs. grossly, pulmonary congestion and edema were the most-common findings. enlarged and congested livers and spleens were also frequently observed. microscopically, there was edema, fibrin exudation, congesti ...200819166069
[occurrence of ixodes loricatus neumann, 1899 (acari: ixodidae) parasitizing didelphis albiventris (lund, 1841) (didelphimorphia: didelphidae) in campo grande, ms].this is first report of occurence of ixodes in the state of mato grosso do sul and first description of ixodes loricatus neumann, 1899, parasitizing didelphis albiventris in campo grande, ms, brazil.200819245763
hosts, distribution and genetic divergence (16s rdna) of amblyomma dubitatum (acari: ixodidae).we supply information about hosts and distribution of amblyomma dubitatum. in addition, we carry out an analysis of genetic divergence among specimens of a. dubitatum from different localities and with respect to other neotropical amblyomma species, using sequences of 16s rdna gene. although specimens of a. dubitatum were collected on several mammal species as cattle horse, tapirus terrestris, mazama gouazoubira, tayassu pecari, sus scrofa, cerdocyon thous, myocastor coypus, allouata caraya, glo ...201020084537
genome survey sequence analysis and identification of homologs of major surface protease (gp63) genes in trypanosoma this study, 222 genome survey sequences were generated for trypanosoma rangeli strain p07 isolated from an opossum (didelphis albiventris) in minas gerais state, brazil. t. rangeli sequences were compared by blastx (basic local alignment search tool x) analysis with the assembled contigs of leishmania braziliensis, leishmania infantum, leishmania major, trypanosoma brucei, and trypanosoma cruzi. results revealed that 82% (182/222) of the sequences were associated with predicted proteins descr ...201020420528
trichinella infection in wild animals from endemic regions of argentina.natural infection with trichinella has been described in more than 150 mammalian species. however, few reports of trichinella infection in wild animals have come from argentina. in this study, muscle tissue was obtained from wild animals in argentina with the aim of evaluating the presence of trichinella. a total of 169 muscle samples were collected to determine the presence of trichinella larvae by artificial digestion. the 169 muscle samples originated from 12 species including 36 opossums (di ...201020424859
search for mycobacterium leprae in wild mammals.leprosy is still a worldwide public health problem. brazil and india show the highest prevalence rates of the disease. natural infection of armadillos dasypus novemcinctus with mycobacterium leprae has been reported in some regions of the united states. identification of bacilli is difficult, particularly due to its inability to grow in vitro. the use of molecular tools represents a fast and sensitive alternative method for diagnosis of mycobacteriosis. in the present study, the diagnostic metho ...201020428654
staphylococcus aureus mastitis in a white-eared opossum (didelphis albiventris) in the atlantic forest of northeast brazil.this is the first brief communication of clinical mastitis caused by staphylococcus aureus occurring in a white-eared opossum (didelphis albiventris) captured in a remnant of the atlantic forest in northeast brazil. the bacterium was cultured from a milk sample by using sheep blood and levine agars. phenotyping of s aureus was performed with coagulase biochemical tests, dnase agar, mannitol agar, and anaerobic glucose and mannitol fermentation.201020945655
leishmania spp. in didelphis albiventris and micoureus paraguayanus (didelphimorphia: didelphidae) of brazil.leishmaniasis is kept in nature by the participation of several animal species. this study evaluated the presence of leishmania spp. in skin samples of free-ranging marsupials micoureus paraguayanus (n=95) and didelphis albiventris (n=191), captured in morro do diabo state park and in sections of its surrounding forest, in the region of pontal do paranapanema, são paulo state, brazil. the samples were tested for the presence of kdna of leishmania spp. by polymerase chain reaction (pcr) and by re ...201021144664
prevalence of antibodies against toxoplasma gondii among brazilian white-eared opossums (didelphis albiventris).considering that toxoplasma gondii is a parasite of global importance which affects several animal species including humans, the current study aimed to investigate the prevalence of antibodies against t. gondii among 72 white-eared opossums (didelphis albiventris) from botucatu municipality (22-¦53's 48-¦26'w), s+úo paulo state, brazil. the investigation was carried out from january 2008 to december 2009, when the animals had their blood samples collected and subjected to the modified agglutinat ...201121435790
Investigation of Chagas disease in four periurban areas in northeastern Brazil: epidemiologic survey in man, vectors, non-human hosts and reservoirs.Chagas disease was investigated in four periurban areas of Ceará state, northeastern Brazil, through serological, parasitological and molecular methods in humans, reservoirs and vectors. A cross-sectional survey revealed a seroprevalence rate of 1.2% (13/1076 residents, six also proving positive through PCR). Trypanosoma cruzi infection was not detected in children under 10 years old. Triatoma pseudomaculata prevailed in the peridomiciles: 63 specimens, 69% (34/49) infected with trypanosomatids. ...201122136953
epidemiological factors related to the transmission risk of trypanosoma cruzi in a quilombola community, state of mato grosso do sul, brazil.this work was an epidemiological investigation of the risk of trypanosoma cruzi transmission in the rural quilombola community of furnas do dionízio, state of mato grosso do sul, brazil.201122031072
Life-cycle and host preference of Amblyomma ovale (Acari: Ixodidae) under laboratory conditions.This study evaluated for the first time the life cycle of Amblyomma ovale in the laboratory. For this purpose, larvae and nymphs were exposed to Gallus gallus (chickens), Cavia porcellus (guinea pigs), Rattus norvegicus (wistar rats), Oryctolagus cuniculus (domestic rabbits), Calomys callosus (vesper mouse), and Didelphis albiventris (white-eared opossum). Nymphs were also exposed to Nectomys squamipes (South American water rat). Adult ticks were fed on dogs. The life-cycle of A. ovale in labora ...201122113779
immunocytochemical demonstration of insulin in the mesonephros and metanephros of the brazilian opossum didelphis albiventris).the presence of insulin the brush border and apical pole of the cells lining the pouch opossum mesonephric and metanephric proximal tubules was demonstrated by immunofluorescence and immunoperoxidase techniques. positive result for insulin was also observed in the tufts of capillaries of some metanephric corpuscles.20143913345
sandflies (diptera: psychodidae) associated with opossum nests at urban sites in southeastern brazil: a risk factor for urban and periurban zoonotic leishmania transmission?sandflies associated with opossum nests are reported for the first time in the yards of residences located in the urban area of the municipality of monte mor, situated in the metropolitan region of campinas, state of são paulo, brazil. eleven specimens of evandromyia cortelezzii and one of evandromyia lenti were captured in two didelphis albiventris nests. ev. cortelezzii is considered a secondary vector species for the transmission of leishmania (viannia) braziliensis and leishmania (leishmania ...024789554
leishmania chagasi in opossums (didelphis albiventris) in an urban area endemic for visceral leishmaniasis, campo grande, mato grosso do sul, brazil.we investigated the occurrence of leishmania infantum chagasi in didelphis albiventris opossums at a wild animal rehabilitation center in the city of campo grande, brazil. a total of 54 opossums were tested for l. i. chagasi infection in peripheral blood and bone marrow samples. the samples were analyzed by direct examination, culturing in a specific medium, and polymerase chain reaction-restriction fragment length polymorphism. leishmania i. chagasi dna was detected by polymerase chain reaction ...201222802435
domestic, peridomestic and wild hosts in the transmission of trypanosoma cruzi in the caatinga area colonised by triatoma brasiliensis.the role played by different mammal species in the maintenance of trypanosoma cruzi is not constant and varies in time and place. this study aimed to characterise the importance of domestic, wild and peridomestic hosts in the transmission of t. cruzi in tauá, state of ceará, caatinga area, brazil, with an emphasis on those environments colonised by triatoma brasiliensis. direct parasitological examinations were performed on insects and mammals, serologic tests were performed on household and out ...201425410992
serological evidence of exposure to tick-borne agents in opossums (didelphis spp.) in the state of são paulo, brazil.this work involved a serological investigation of tick-borne pathogens in opossums in eight municipalities of the state of são paulo, brazil. serum samples from 109 opossums (91 didelphis aurita and 18 didelphis albiventris) were tested to detect antibodies to rickettsia rickettsii (taiaçu strain, 1:64 cut-off) and ehrlichia canis (são paulo strain, 1:40 cut-off), by indirect immunofluorescence assay (ifa); and against borrelia burgdorferi (strain g39/40) by enzyme-linked immunosorbent assay (el ...201627276663
small mammals as hosts of leishmania spp. in a highly endemic area for zoonotic leishmaniasis in north-eastern brazil.leishmania parasites cause leishmaniasis in humans and animals worldwide. these parasites are transmitted by phlebotomine sand flies, which become infected upon feeding on an infected mammalian host. we assessed the occurrence of leishmania infection in small mammals in an area of cutaneous and visceral leishmaniasis endemicity.201323868744
molecular detection of leishmania spp. in road-killed wild mammals in the central western area of the state of são paulo, brazil.road-killed wild animals have been classified as sentinels for detecting such zoonotic pathogens as leishmania spp., offering new opportunities for epidemiological studies of this infection.201424963288
expanding the knowledge about leishmania species in wild mammals and dogs in the brazilian savannah.wild, synanthropic and domestic mammals act as hosts and/or reservoirs of several leishmania spp. studies on possible reservoirs of leishmania in different areas are fundamental to understand host-parasite interactions and develop strategies for the surveillance and control of leishmaniasis. in the present study, we evaluated the leishmania spp. occurrence in mammals in two conservation units and their surroundings in brasília, federal district (fd), brazil.201525889365
[mycobacterium bovis in wildlife of the dairy regions of santa fe (argentina)].control eradication campaigns of bovine tuberculosis based on the «test and slaughter» approach were successful in many countries and regions; however, in some areas the infection persists and one of the main reasons is mycobacterium bovis infection in wild life species. argentina has applied the same approach since 1999, achieving progress in dairy cattle herds. nonetheless, the wildlife role has never been investigated. the objective of this study was to determine if wildlife from the santa fe ...201526376835
rickettsial infection in animals, humans and ticks in paulicéia, brazil.a previous study in paulicéia municipality, south-eastern brazil, reported 9.7% of the amblyomma triste ticks to be infected by rickettsia parkeri, a bacterial pathogen that causes spotted fever in humans. these a. triste ticks were shown to be associated with marsh areas, where the marsh deer blastocerus dichotomus is a primary host for this tick species. during 2008-2009, blood serum samples were collected from 140 horses, 41 dogs, 5 opossums (didelphis albiventris) and 26 humans in farms from ...201525643912
genotyping of toxoplasma gondii and sarcocystis spp. in road-killed wild mammals from the central western region of the state of são paulo, brazil.road-killed wild animals host zoonotic pathogens such as toxoplasma gondii, offering a new opportunity for the epidemiological study of these infectious organisms.201627812655
toxoplasma gondii in domestic and wild animals from forest fragments of the municipality of natal, northeastern brazil.toxoplasmosis stands out as a global disease that has felines as definitive hosts. in the municipality of natal, rio grande do norte state, brazil, two parks are notable for their ecological and social importance. this study aimed to investigate the presence of toxoplasma gondii in short hair cats, bats and small non-volant mammals in these two ecological reserves. altogether, biological samples were obtained from 154 mammals, 92 wild animals from both areas and 62 domestic cats of the parque da ...201725517529
hosts and vectors of trypanosoma cruzi discrete typing units in the chagas disease endemic region of the paraguayan trypanosoma cruzi transmission persists in the gran chaco region, which is considered hyperendemic for chagas disease. understanding domestic and sylvatic transmission cycles and therefore the relationship between vectors and mammalian hosts is crucial to designing and implementing improved effective control strategies. here we describe the species of triatomine vectors and the sylvatic mammal reservoirs of t. cruzi, in different localities of the paraguayan and bolivian chaco. we identif ...201728179034
a comparative study of trypanosoma cruzi infection in sylvatic mammals from a protected and a disturbed area in the argentine chaco.understanding the complex epidemiology of trypanosoma cruzi transmission cycles requires comparative studies in widely different environments. we assessed the occurrence of t. cruzi infection in sylvatic mammals, their infectiousness to the vector, and parasite genotypes in a protected area of the argentine chaco, and compared them with information obtained similarly in a nearby disturbed area. a total of 278 mammals from >23 species in the protected area were diagnosed for t. cruzi infection us ...201626708994
domestic, peridomestic and wild hosts in the transmission of trypanosoma cruzi in the caatinga area colonised by triatoma brasiliensis.the role played by different mammal species in the maintenance of trypanosoma cruzi is not constant and varies in time and place. this study aimed to characterise the importance of domestic, wild and peridomestic hosts in the transmission of t. cruzi in tauá, state of ceará, caatinga area, brazil, with an emphasis on those environments colonised by triatoma brasiliensis. direct parasitological examinations were performed on insects and mammals, serologic tests were performed on household and out ...201425165976
molecular identification of trypanosomatids in wild animals.diverse wild animal species can be reservoirs of zoonotic flagellate parasites, which can cause pathologic chagas disease. the present study aimed to detect the natural occurrence of flagellate parasites through direct microscopic examination of the parasites in blood samples and through pcr of whole blood and blood culture (haemoculture) samples from 38 captive and 65 free-living wild animals in the centre for conservation of wild fauna (ccwf), an area endemic for leishmaniasis. for this study, ...201424636787
evaluation of natural foci of panstrongylus megistus in a forest fragment in porto alegre, state of rio grande do sul, brazil.panstrongylus megistus is commonly found in wild environments of the state of rio grande do sul, brazil. the aim of this study was to characterize the network of refuges used by triatomine in a forest fragment of porto alegre and to identify trypanosoma cruzi infection, associated hosts and the epidemiological importance of both hosts and triatomines.201324270248
trans-sialidase inhibition assay detects trypanosoma cruzi infection in different wild mammal species.the detection of trypanosoma cruzi infection in mammals is crucial for understanding the eco-epidemiological role of the different species involved in parasite transmission cycles. xenodiagnosis (xd) and hemoculture (hc) are routinely used to detect t. cruzi in wild mammals. serological methods are much more limited because they require the use of specific antibodies to immunoglobulins of each mammalian species susceptible to t. cruzi. in this study we detected t. cruzi infection by trans-sialid ...201323930975
new sylvatic hosts of trypanosoma cruzi and their reservoir competence in the humid chaco of argentina: a longitudinal study.a four-year longitudinal study of the structure of sylvatic transmission cycles of trypanosoma cruzi, reservoir host competence and parasite discrete typing units was conducted in a disturbed rural area of the humid chaco in argentina. among 190 mammals examined by xenodiagnosis and polymerase chain reaction amplification, the composite prevalence of infection was substantially higher in dasypus novemcinctus armadillos (57.7%) and didelphis albiventris opossums (38.1%) than in euphractus sexcinc ...201323530075
direct molecular identification of trypanosoma cruzi discrete typing units in domestic and peridomestic triatoma infestans and triatoma sordida from the argentine chaco.we assessed the distribution of trypanosoma cruzi discrete typing units (dtus) in domestic and peridomestic triatoma infestans and triatoma sordida specimens collected in a well-defined rural area in pampa del indio, northeastern argentina. microscopically-positive bugs were randomly selected with a multi-level sampling design, and dtus were identified using direct pcr strategies. tcvi predominated in 61% of 69 t. infestans and in 56% of 9 t. sordida. tcv was the secondary dtu in t. infestans (1 ...201223036510
microhabitat of small mammals at ground and understorey levels in a deciduous, southern atlantic forest.each animal species selects specific microhabitats for protection, foraging, or micro-climate. to understand the distribution patterns of small mammals on the ground and in the understorey, we investigated the use of microhabitats by small mammals in a deciduous forest of southern brazil. ten trap stations with seven capture points were used to sample the following microhabitats: liana, fallen log, ground litter, terrestrial ferns, simple-trunk tree, forked tree, and piper sp. shrubs. seven fiel ...201323828340
association patterns of ticks (acari: ixodida: ixodidae, argasidae) of small mammals in cerrado fragments, western brazil.the present study describes ticks associated with small mammals and analyzes the aggregation patterns according to seasonal and host variations in the cerrado biome, central-western brazil. small mammals were systematically captured in 54 woodland fragments from february 2012 to july 2013. a total of 1,040 animals belonging to eight marsupial and 12 rodent species were captured; 265 animals were parasitized by eight tick species (in decreasing order of abundance): ornithodoros mimon, amblyomma c ...201525633262
the sylvatic transmission cycle of trypanosoma cruzi in a rural area in the humid chaco of argentina.little is known about the sylvatic transmission cycle of trypanosoma cruzi in the gran chaco ecoregion. we conducted surveys to identify the main sylvatic hosts of t. cruzi, parasite discrete typing units and vector species involved in pampa del indio, a rural area in the humid argentinean chaco. a total of 44 mammals from 14 species were captured and examined for infection by xenodiagnosis and polymerase chain reaction amplification of the hyper-variable region of kinetoplast dna minicircles of ...201222771688
first finding of trypanosoma cruzi ii in vampire bats from a district free of domestic vector-borne transmission in northeastern argentina.establishing the putative links between sylvatic and domestic transmission cycles of trypanosoma cruzi, the etiological agent of chagas disease, is of public health relevance. we conducted three surveys to assess t. cruzi infection in wild mammals from a rural and a preserved area in misiones province, northeastern argentina, which had recently been declared free of vector- and blood-borne transmission of human t. cruzi infection. a total of 200 wild mammals were examined by xenodiagnosis (xd) a ...201627220254
toxocara cati (nematoda: ascarididae) in didelphis albiventris (marsupialia: didelphidae) from brazil: a case of pseudoparasitism.eggs of toxocara cati were found in the feces of didelphis albiventris from a peridomestic urban environment in brazil. negative fecal tests following short-term captivity of the opossums, as well as the absence of ascaridids during necropsy, suggest the occurrence of pseudoparasitism. implications of the findings for the epidemiology of toxocariasis are discussed.201725517533
molecular characterization and development of sarcocystis speeri sarcocysts in gamma interferon gene knockout mice.the north american opossum (didelphis virginiana) is the definitive host for at least three named species of sarcocystis: sarcocystis falcatula, sarcocystis neurona and sarcocystis speeri. the south american opossums (didelphis albiventris, didelphis marsupialis and didelphis aurita) are definitive hosts for s. falcatula and s. lindsayi. the sporocysts of these sarcocystis species are similar morphologically. they are also not easily distinguished genetically because of the difficulties of dna e ...201526303093
necrotizing meningoencephalitis caused by sarcocystis falcatula in bare-faced ibis (phimosus infuscatus).the infection by s. falcatula is commonly associated with respiratory disease in captive psittacine birds, with a few case reports of this protozoan causing encephalitis in wild birds. we describe the clinical, pathological, and molecular aspects of an infection by s. falcatula in a bare-faced ibis (phimosus infuscatus). clinically, wing paralysis and mild motor incoordination were observed. at necropsy, the telencephalic cortex showed multifocal to coalescing yellowish soft areas. histologicall ...201727915419
first isolation of leptospira interrogans from lycalopex griseus (south american gray fox) in argentina shows new mlva identify carriers of leptospira spp. in argentina, wild animals were trapped in buenos aires province during three nights, capturing 12 didelphis albiventris (white-eared opossum), six chaetophractus villosus (big hairy armadillo), five lycalopex griseus (south american gray fox), and two conepatus chinga (molina's hog-nosed skunk). all were tested by microscopic agglutination test, and five (two gray foxes, two armadillos, and one skunk) were positive for leptospira interrogans serovars cani ...201323307384
leptospira borgpetersenii from free-living white-eared opossum (didelphis albiventris): first isolation in brazil.leptospirosis is a zoonotic disease that occurs all over the world, caused by bacteria of the genus leptospira. marsupial and didelphidae families are considered susceptible to infection caused by a wide range of leptospira serovars for which they serve as reservoirs. thirty-three free-living white-eared opossums (didelphis albiventris) were captured in southern brazil and bodily fluids were collected. from the urine samples it was possible to obtain an isolate identified as leptospira borgpeter ...201222897870
leishmania spp. in didelphis spp. from northeastern brazil.the synanthropic behavior of marsupials of the genus didelphis in endemic areas of leishmaniasis suggests that these animals may play an important role in the epidemiology of this infection. the aim of the present study was to detect leishmania spp. dna in didelphis albiventris (white-eared opossum) and didelphis aurita (big-eared opossum) living in forested and peridomestic areas of northeastern brazil. blood samples were collected from 25 animals (23 d. albiventris and 2 d. aurita ) by cardiac ...201627691942
serologic evidence of canine parvovirus in domestic dogs, wild carnivores, and marsupials in the argentinean chaco.the transmission of pathogens between domestic dogs and generalist wildlife species may be modified by environmental degradation, biodiversity losses, host densities, and increased contact rates in remnant forest patches. a serologic survey of canine parvovirus (cpv) in rural domestic dogs and wild mammals was conducted in two neighboring rural areas (disturbed and protected) from pampa del indio, northeastern argentina, between 2008 and 2011. a total of 174 domestic dogs and 26 wild mammals-4 c ...201425314822
biochemical, pharmacological, and structural characterization of new basic pla2 bbil-tx from bothriopsis bilineata snake venom.bbil-tx, a pla2, was purified from bothriopsis bilineata snake venom after only one chromatographic step using rp-hplc on μ-bondapak c-18 column. a molecular mass of 14243.8 da was confirmed by q-tof ultima api esi/ms (tof ms mode) mass spectrometry. the partial protein sequence obtained was then submitted to blastp, with the search restricted to pla2 from snakes and shows high identity values when compared to other pla2s. pla2 activity was presented in the presence of a synthetic substrate and ...201223509754
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