| l-asparaginase-producing streptomyces from the soil of kuwait. | five actinomycete isolates (all belonged to the genus streptomyces), capable of producing detectable amounts of l-asparaginase, were isolated from the soil of kuwait after enrichment. the three most potent enzyme producers were identified as different strains of streptomyces collinus. factors affecting enzyme production by the strongest strain were examined. synthetic media with asparagine as a nitrogen source stimulated more enzyme production than natural media. starch and asparagine at final c ... | 1979 | 44599 |
| biosynthesis of ansatrienin by streptomyces collinus: cell-free transformations of cyclohexene- and cyclohexadienecarboxylic acids. | cell-free extracts of streptomyces collinus were tested with various cyclohexene- and cyclohexadienecarboxylic acids in order to determine the latter stages of the conversion of shikimic acid to cyclohexanecarboxylic acid. it was demonstrated that the final three steps of this process involve reduction of the alpha,beta-double bond of 1(6),2-cyclohexadienylcarbonyl coa, an isomerization of the double bond of the resulting 2-cyclohexenylcarbonyl coa to afford 1-cyclohexenylcarbonyl coa, and a sub ... | 1992 | 1577668 |
| purification and characterization of a novel enoyl coenzyme a reductase from streptomyces collinus. | a novel nadph-dependent enoyl reductase, catalyzing the conversion of 1-cyclohexenylcarbonyl coenzyme a (1-cyclohexenylcarbonyl-coa) to cyclohexylcarbonyl-coa, was purified to homogeneity from streptomyces collinus. this enzyme, a dimer with subunits of identical m(r) (36,000), exhibits a km of 1.5 +/- 0.3 microm for nadph and 25 +/- 3 microm for 1-cyclohexenylcarbonyl-coa. it has a ph optimum of 7.5, is most active at 30 degrees c, and is inhibited by both divalent cations and thiol reagents. t ... | 1992 | 1597409 |
| resorthiomycin, a novel antitumor antibiotic. i. taxonomy, isolation and biological activity. | resorthiomycin, a novel antitumor antibiotic, was isolated from the fermentation broth of a strain of streptomyces collinus by ethyl acetate extraction, silica gel chromatography and hplc. resorthiomycin exhibited an in vitro cytotoxic activity against mouse leukemia l5178y cells (ic50, 15.5 micrograms/ml) and also inhibited the clonogenic activity of a multidrug-resistant mutant of human hepatoma plc/prf/5 cells to a greater extent than that of the parental cells. on the other hand, this antibi ... | 1990 | 2155895 |
| biosynthesis of the ansamycin antibiotic ansatrienin (mycotrienin) by streptomyces collinus. | the biosynthesis of ansatrienin (mycotrienin) has been studied in radioactive and stable isotope feeding experiments with streptomyces collinus tü 1892. the m-c7n unit of the ansa ring is efficiently and specifically derived from 3-amino-5-hydroxybenzoic acid; shikimic acid is not incorporated into this part of the molecule but does label the cyclohexanecarboyxlic acid moiety, providing all seven of its carbon atoms. incorporation of methionine confirms origin of the methoxy group by transmethyl ... | 1987 | 3598593 |
| elastase-producing microorganisms in horse lungs: their possible role in the pathogenesis of chronic pulmonary disease in the horse. | seventeen out of 21 horses had pulmonary microbial organisms which reached considerable numbers in seven cases. elastase-producing microorganisms from the environment (streptomyces species and to a lesser extent bacillus species) constituted 22 per cent to 99 per cent (mean 79 per cent) of the total growth. there was a considerable number of microorganisms with in vitro-produced elastases which were not or only slightly affected by horse serum. there was no correlation between numbers of organis ... | 1986 | 3639820 |
| a new dipeptide antibiotic from streptomyces collinus, lindenbein. | | 1972 | 4624614 |
| protein synthesis elongation factor eftu from streptomyces collinus producing kirromycin. | | 1982 | 6756407 |
| ansatrienin a2 and a3: minor components of the ansamycin complex produced by streptomyces collinus. | | 1983 | 6833138 |
| sequencing of the tuf1 gene and the phosphorylation pattern of ef-tu1 during development and differentiation in streptomyces collinus producing kirromycin. | we have cloned and sequenced the tuf1 gene from a kirromycin-producing strain of streptomyces collinus. the gene encodes a polypeptide of 396 amino acid residues with a molecular weight of 43,849. the protein shows 97% identity with ef-tu1 of s. coelicolor and is sensitive to kirromycin. ef-tu-dependent translation of poly(u) was reduced to 50% in the presence of 0.25 microm kirromycin. using high resolution two-dimensional electrophoresis and specific immunodetection with monoclonal antibodies ... | 1995 | 7646499 |
| biosynthetic studies on the origin of the cyclohexanecarboxylic acid moiety of ansatrienin a and omega-cyclohexyl fatty acids. | feeding experiments with [2,6,10,10-2h4]chorismate in streptomyces collinus (ansatrienin a) and alicyclobacillus acidocaldarius (omega-cyclohexyl fatty acids), and inhibitor experiments with glyphosate in the latter organism, have shown that the biosynthesis of cyclohexanecarboxylic acid branches off from the shikimate pathway at a point prior to enolpyruvylshikimate 3-phosphate, either at shikimate or shikimate 3-phosphate. | 1994 | 8201312 |
| purification of crotonyl-coa reductase from streptomyces collinus and cloning, sequencing and expression of the corresponding gene in escherichia coli. | a crotonyl-coa reductase (ec 1.3.1.38, acyl-coa:nadp+ trans-2-oxidoreductase) catalyzing the conversion of crotonyl-coa to butyryl-coa has been purified and characterized from streptomyces collinus. this enzyme, a dimer with subunits of identical mass (48 kda), exhibits a km = 18 microm for crotonyl-coa and 15 microm for nadph. the enzyme was unable to catalyze the reduction of any other enoyl-coa thioesters or to utilize nadh as an electron donor. a highly effective inhibition by straight-chain ... | 1995 | 8521864 |
| cloning and characterization of the gene encoding 1-cyclohexenylcarbonyl coenzyme a reductase from streptomyces collinus. | we report the cloning of the gene encoding the 1-cyclohexenylcarbonyl coenzyme a reductase (chca) of streptomyces collinus, an enzyme putatively involved in the final reduction step in the formation of the cyclohexyl moiety of ansatrienin from shikimic acid. the cloned gene, with a proposed designation of chca, encodes an 843-bp open reading frame which predicts a primary translation product of 280 amino acids and a calculated molecular mass of 29.7 kda. highly significant sequence similiarity e ... | 1996 | 8955309 |
| in vivo and in vitro effects of thiolactomycin on fatty acid biosynthesis in streptomyces collinus. | a stable-isotope assay was used to analyze the effectiveness of various perdeuterated short-chain acyl coenzyme a (acyl-coa) compounds as starter units for straight- and branched-chain fatty acid biosynthesis in cell extracts of streptomyces collinus. in these extracts perdeuterated isobutyryl-coa was converted to isopalmitate (a branched-chain fatty acid), while butyryl-coa was converted to palmitate (a straight-chain fatty acid). these observations are consistent with previous in vivo analyses ... | 1997 | 9190803 |
| a novel alternate anaplerotic pathway to the glyoxylate cycle in streptomycetes. | ccr encoding crotonyl coenzyme a (coa) reductase (ccr), which catalyzes the conversion of crotonyl-coa to butyryl-coa in the presence of nadph, was previously cloned from streptomyces collinus. we now report that a complete open reading frame, designated meaa, is located downstream from ccr. the predicted gene product showed 35% identity with methylmalonyl-coa mutases from various sources. in addition, the predicted amino acid sequences of s. collinus ccr and meaa exhibit strong similarity to th ... | 1997 | 9260959 |
| protein kinase associated with ribosomes phosphorylates ribosomal proteins of streptomyces collinus. | protein kinase activity associated with ribosomes of a kirromycin-producing strain of streptomyces collinus was detected. the enzyme utilizes [gamma-32p]atp to phosphorylate proteins, yielding acid-stable phosphoamino acids. two-dimensional electrophoresis of proteins from a crude ribosomal fraction revealed 17 phosphoproteins. eleven of the phosphoproteins exhibited electrophoretic mobility identical to that of s. collinus ribosomal proteins s3, s4, s12, s13, s14, s18, l2, l7, l16, l17, and l23 ... | 1997 | 9299515 |
| pattern of phosphoproteins during cell differentiation in streptomyces collinus. | transition from vegetative cells to aerial mycelium and spores of streptomyces collinus is accompanied by changes in the pattern of proteins phosphorylated. preparation from spores exhibits lower phosphorylation activity than those of vegetative cells and aerial mycelium. phosphorylation of proteins from aerial mycelium was markedly stimulated by the presence of mn2+. our data indicate that phosphorylation of proteins on ser/thr residues is involved in transition of vegetative cells to aerial my ... | 1998 | 9453167 |
| characterization of ftsz gene and its protein product from streptomyces collinus producing kirromycin. | the ftsz protein is required for septation and conversion of aerial mycelium into chains of streptomycete spores. we have cloned and sequenced the ftsz gene from streptomyces collinus. the ftsz of s. collinus is not in juxtaposition with ftsa and ipxc as in escherichia coli or bacillus subtilis. the gene encodes a polypeptide of 402 amino acid residues with a molecular mass of 41.3 kda. n-terminus shares a high level of sequence similarity with ftsz of s. coelicolor and s. griseus, respectively. ... | 1998 | 9712736 |
| biosynthesis of ansatrienin (mycotrienin) and naphthomycin. identification and analysis of two separate biosynthetic gene clusters in streptomyces collinus tü 1892. | the polyketide chains of the two ansamycin antibiotics, ansatrienin (mycotrienin) and naphthomycin produced by streptomyces collinus are assembled using 3-amino-5-hydroxybenzoic acid (ahba) as a starter unit. the gene encoding ahba synthase, an enzyme which catalyzes the final step of ahba biosynthesis in the recently discovered aminoshikimate pathway, has been used to identify two separate antibiotic biosynthetic gene clusters in s. collinus. in one of these clusters, analysis of approximately ... | 1999 | 10103039 |
| role of crotonyl coenzyme a reductase in determining the ratio of polyketides monensin a and monensin b produced by streptomyces cinnamonensis. | the ccr gene, encoding crotonyl coenzyme a (coa) reductase (ccr), was cloned from streptomyces cinnamonensis c730.1 and shown to encode a protein with 90% amino acid sequence identity to the ccrs of streptomyces collinus and streptomyces coelicolor. a ccr-disrupted mutant, s. cinnamonensis l1, was constructed by inserting the hyg resistance gene into a unique bglii site within the ccr coding region. by use of the erme* promoter, the s. collinus ccr gene was expressed from plasmids in s. cinnamon ... | 1999 | 10542184 |
| isolation and characterization of dcw cluster from streptomyces collinus producing kirromycin. | a 4.5-kb bamhi fragment of chromosomal dna of streptomyces collinus containing gene ftsz was cloned and sequenced. upstream of ftsz are localized genes ftsq, murg, and ftsw, and downstream is yfih. gene ftsa is not adjacent to ftsz or other genes of the cloned fragment. protein ftsz was isolated and characterized with respect to its binding to gtp and gtpase activity. the binding of gtp to ftsz was ca(2+) or mg(2+) dependent with an optimum at 10 mm. the rate of gtp hydrolysis by ftsz was stimul ... | 2000 | 10679194 |
| a novel delta(3),delta(2)-enoyl-coa isomerase involved in the biosynthesis of the cyclohexanecarboxylic acid-derived moiety of the polyketide ansatrienin a. | the side chain of the antifungal polyketide ansatrienin a produced by streptomyces collinus contains a cyclohexanecarboxylic acid (chc) derived moiety. this chc in the coenzyme a activated form (chc-coa) is derived from shikimic acid via a pathway in which the penultimate step is the isomerization of 2-cyclohexenylcarbonyl-coa to 1-cyclohexenylcarbonyl-coa. we have purified a 28 kda 2-cyclohexenylcarbonyl-coa isomerase (chcb) from s. collinus and cloned and sequenced the corresponding chcb gene. ... | 2000 | 10858310 |
| identification of a cyclohexylcarbonyl coa biosynthetic gene cluster and application in the production of doramectin. | the side chain of the antifungal antibiotic ansatrienin a from streptomyces collinus contains a cyclohexanecarboxylic acid (chc)-derived moiety. this moiety is also observed in trace amounts of omega-cyclohexyl fatty acids (typically less than 1% of total fatty acids) produced by s. collinus. coenzyme a-activated chc (chc-coa) is derived from shikimic acid through a reductive pathway involving a minimum of nine catalytic steps. five putative chc-coa biosynthetic genes in the ansatrienin biosynth ... | 2000 | 10973220 |
| characterization of the rplb gene from streptomyces collinus and its protein product by mass spectrometry. | ribosomal protein l2 is the largest protein components of 50s subunits. the protein is implicated in peptidyl transferase activity and binds to functionally important domains of 23s rrna. the rplb gene, which codes for ribosomal protein l2 was cloned from streptomyces collinus. the gene rplb was isolated from bamhi fragment (3.0 kb) of chromosomal dna possessing two partial and four complete orf's in the order from 5' to 3': rplc, rpld, rplw, rplb, rpss, and rplv. the gene organization correspon ... | 2001 | 11478805 |
| a streptomyces collinus thiolase with novel acetyl-coa:acyl carrier protein transacylase activity. | acetyl-coa:acyl carrier protein (acp) transacylase (act) activity has been demonstrated for the 3-ketoacyl-acp synthase iii (kasiii) which initiates fatty acid biosynthesis in the type ii dissociable fatty acid synthases of plants and bacteria. several lines of evidence have indicated the possibility of act activity being associated with proteins other than kasiii. using a crude extract of streptomyces collinus, we have resolved from kasiii an additional protein with act activity and subsequentl ... | 2001 | 11570897 |
| changes in ribosome function induced by protein kinase associated with ribosomes of streptomyces collinus producing kirromycin. | protein kinase associated with ribosomes of streptomycetes phosphorylates 11 ribosomal proteins. phosphorylation activity of protein kinase reaches its maximum at the end of exponential phase of growth. when (32)p-labeled cells from the end of exponential phase of growth were transferred to a fresh medium, after 2 h of cultivation ribosomal proteins lost more than 90% of (32)p and rate of polypeptide synthesis increases twice. protein kinase cross-reacting with antibody raised against protein ki ... | 2001 | 11716492 |
| crotonyl-coenzyme a reductase provides methylmalonyl-coa precursors for monensin biosynthesis by streptomyces cinnamonensis in an oil-based extended fermentation. | it is demonstrated that crotonyl-coa reductase (ccr) plays a significant role in providing methylmalonyl-coa for monensin biosynthesis in oil-based 10-day fermentations of streptomyces cinnamonensis. under these conditions s. cinnamonensis l1, a derivative of a high-titre producing industrial strain c730.1 in which ccr has been insertionally inactivated, produces only 15 % of the monensin yield. labelling of the coenzyme a pools using [3h]-beta-alanine and analysis of intracellular acyl-coas in ... | 2004 | 15470123 |
| multiple pathways for acetate assimilation in streptomyces cinnamonensis. | in most bacteria acetate assimilation is accomplished via the glyoxylate pathway. isocitrate lyase (icl) and malate synthase (ms) are two key enzymes of this pathway, which results in the net generation of one molecule of succinyl-coa from two acetyl-coa molecules. genetic and biochemical data have shown that genes encoding these key enzymes are present in streptomycetes, yet there has been no clear demonstration of the importance of these genes to acetate assimilation. in fact, for streptomyces ... | 2006 | 16187095 |
| genetic manipulation of the biosynthetic process leading to phoslactomycins, potent protein phosphatase 2a inhibitors. | phoslactomycins (plms) represent an unusual structural class of natural products secreted by various streptomycetes, containing an alpha,beta-unsaturated delta-lactone, an amino group, phosphate ester, conjugated diene and a cyclohexane ring. phosphazomycins, phospholines and leustroducsins contain the same structural moieties, varying only in the acyl substituent at the c-18 hydroxyl position. these compounds possess either antifungal or antitumor activities or both. the antitumor activity of t ... | 2006 | 16609856 |
| molecular analysis of the kirromycin biosynthetic gene cluster revealed beta-alanine as precursor of the pyridone moiety. | kirromycin is a complex linear polyketide that acts as a protein biosynthesis inhibitor by binding to the bacterial elongation factor tu. the kirromycin biosynthetic gene cluster was isolated from the producer, streptomyces collinus tü 365, and confirmed by targeted disruption of essential biosynthesis genes. kirromycin is synthesized by a large hybrid polyketide synthase (pks)/nonribosomal peptide synthetase (nrps) encoded by the genes kirai-kiravi. this complex involves some very unusual featu ... | 2008 | 18291322 |
| the kirromycin gene cluster of streptomyces collinus tü 365 codes for an aspartate-alpha-decarboxylase, kird, which is involved in the biosynthesis of the precursor beta-alanine. | | 2009 | 19609288 |
| tmrna of streptomyces collinus and streptomyces griseus during the growth and in the presence of antibiotics. | streptomycetes are soil microorganisms with the potential to produce a broad spectrum of secondary metabolities. the production of antibiotics is accompanied by a decrease in protein synthesis, which raises the question of how these bacteria survived the transition from the primary to the secondary metabolism. translating ribosomes incapable to properly elongate or terminate polypeptide chain activate bacterial trans-translation system. abundance and stability of the tmrna during growth of strep ... | 2009 | 21261886 |
| the phosphopantetheinyl transferase kirp activates the acp and pcp domains of the kirromycin nrps/pks of streptomyces collinus tü 365. | the main steps in the biosynthesis of complex secondary metabolites such as the antibiotic kirromycin are catalyzed by modular polyketide synthases (pks) and/or nonribosomal peptide synthetases (nrps). during antibiotic assembly, the biosynthetic intermediates are attached to carrier protein domains of these megaenzymes via a phosphopantetheinyl arm. this functional group of the carrier proteins is attached post-translationally by a phosphopantetheinyl transferase (pptase). no experimental evide ... | 2011 | 21401713 |
| evaluation of antagonistic and plant growth promoting activities of chitinolytic endophytic actinomycetes associated with medicinal plants against sclerotium rolfsii in chickpea. | to evaluate the potential of chitinolytic endophytic actinomycetes isolated from medicinal plants in order to diminish the collar rot infestation induced by sclerotium rolfsii in chickpea. | 2016 | 27170067 |
| streptocollin, a type iv lanthipeptide produced by streptomyces collinus tü 365. | lanthipeptides are ribosomally synthesized and post-translationally modified microbial secondary metabolites. here, we report the identification and isolation of streptocollin from streptomyces collinus tü 365, a new member of class iv lanthipeptides. insertion of the constitutive erme* promoter upstream of the lanthipeptide synthetase gene stcl resulted in peptide production. the streptocollin gene cluster was heterologously expressed in s. coelicolor m1146 and m1152 with 3.5- and 5.5-fold incr ... | 2015 | 26437689 |
| identification and activation of novel biosynthetic gene clusters by genome mining in the kirromycin producer streptomyces collinus tü 365. | streptomycetes are prolific sources of novel biologically active secondary metabolites with pharmaceutical potential. s. collinus tü 365 is a streptomyces strain, isolated 1972 from kouroussa (guinea). it is best known as producer of the antibiotic kirromycin, an inhibitor of the protein biosynthesis interacting with elongation factor ef-tu. genome mining revealed 32 gene clusters encoding the biosynthesis of diverse secondary metabolites in the genome of streptomyces collinus tü 365, indicating ... | 2016 | 26433383 |
| complete genome sequence of the kirromycin producer streptomyces collinus tü 365 consisting of a linear chromosome and two linear plasmids. | streptomyces collinus tü 365 (dsmz 40733), isolated from kouroussa (guinea), is the producer of the elfamycin family antibiotic kirromycin, which inhibits bacterial protein biosynthesis by interfering with elongation factor ef-tu. here, we report on the streptomyces collinus tü 365 complete genome sequence of the 8.27 mb chromosome and the two plasmids sco1 and sco2. | 2013 | 24140291 |