| toxicity of a moniliformin-producing strain of fusarium moniliforme var. subglutinans isolated from maize. | | 1977 | 604234 |
| isolation of beauvericin as an insect toxin from fusarium semitectum and fusarium moniliforme var. subglutinans. | nonpolar methylene chloride-soluble extracts from the mycelia of fusarium semitectum and fusarium moniliforme var. subglutinans were toxic to colorado potato beetles. the major toxic metabolite was isolated and found to be the cyclodepsipeptide, beauvericin. this is the first report of the isolation of beauvericin from the genus fusarium. | 1991 | 1749401 |
| toxicity of fusarium moniliforme var. subglutinans for chicks, ducklings, and turkey poults. | corn-based diets contaminated with various concentrations of a moniliformin-producing isolate of fusarium moniliforme var. subglutinans were found to be lethal for chicks, ducklings, and turkey poults. ducklings appeared to be the most sensitive to the lethal effects of the toxic feed. gross lesions were ascites, hydropericardium, and myocardial pallor. microscopic lesions were limited to the heart and liver, and they consisted of degeneration and necrosis of the myocardium and degeneration of h ... | 1989 | 2751566 |
| [study on toxicological mechanism of moniliformin]. | a mycotoxin was extracted and purified from a strain of fusarium moniliforme var. subglutinans isolated from mouldy corn seeds harvested in a serious keshan disease region in shaanxi province. the purification procedure involves water extraction, ion exchange chromatography, desalination and crystallization. its uv spectrum, ir spectrum and nmr are identical with that of the moniliformin. the toxin is highly toxic to young beijing ducklings. the electrocardiogram (ecg) of the duckling was change ... | 1989 | 2800548 |
| moniliformin produced by cultures of fusarium moniliforme var. subglutinans isolated from swine feed. | feed samples from iowa suspected of causing vomiting and enlarged vulva as well as mortalities of swine were examined for toxigenic fungi and mycotoxins fusarium moniliforme sheldon and f. moniliforme sheldon var. subglutinans wollenew. & reink. accounted for 43% and 18.5%, respectively, of the total count of 4.75 x 10(5) propagules filamentous fungi per gram of swine feed, but representatives of various penicillium spp. and aspergillus spp. were also found. eight isolates of f. moniliforme var. ... | 1986 | 2945990 |
| liquid chromatographic determination and stability of the fusarium mycotoxin moniliformin in cereal grains. | moniliformin is a mycotoxin produced by fusarium subglutinans and other fusarium species. a rapid, liquid chromatographic method for its determination in corn and wheat is described. samples are extracted in acetonitrile-water (95 + 5); following defatting with n-hexane, an aliquot of the extract is evaporated and cleaned up on small c18 and neutral alumina columns successively. reverse-phase liquid chromatography (lc) is conducted on a c18 column with 10 or 15% methanol or acetonitrile in aqueo ... | 1987 | 3680124 |
| fusarin c production by north american isolates of fusarium moniliforme. | a liquid culture medium was developed to screen north american isolates of fusarium moniliforme sheldon and fusarium subglutinans (wollenw. and reink.) nelson, toussoun, and marasas for their ability to produce fusarin c. parameters which were important for the optimal biosynthesis of fusarin c included ph (3.0 to 4.0), aeration, and sugar concentration (30 to 40%). of seven sugars tested, sucrose and glucose were the best carbohydrate sources for mycotoxin production, resulting in levels of fus ... | 1986 | 3954349 |
| inhibition of fusarium moniliforme var. subglutinans, the causal agent of pine pitch canker, by the soil bacterium arthrobacter sp. | a species of arthrobacter was recovered during culture of the causal organism of pitch canker of southern pines. fusarium moniliforme var. subglutinans (fms). arthrobacter is a relatively common soil bacterium and is lytic to several fungal pathogens in the soil. soil samples from two seed orchards with pitch canker and one from a healthy pine plantation all yielded arthrobacter. these isolates were evaluated for their ability to inhibit the growth of fms isolates from pitch canker tissue and fr ... | 1981 | 7214230 |
| two cases of exogenous endophthalmitis due to fusarium moniliforme and pseudomonas species as associated aetiological agents. | perforative eye injuries in two patients were complicated by fulminant endophthalmitis. in both patients the cornea was free of micro-organisms. vitrectomy of the posterior pars plana was performed and the contents of the vitreous body were examined mycologically and bacteriologically. fusarium moniliforme var. subglutinans and pseudomonas species were isolated from both patients. fungal and bacterial organisms penetrated the vitreous body via metal foreign bodies. | 1993 | 7935580 |
| ear-rot fungi and mycotoxins in south african corn of the 1989 crop exported to taiwan. | a shipment of south african corn (1989) exported to taiwan, was analyzed for various ear-rot fungi and fusarium mycotoxins. two sets of samples, one from the points of origin in south africa prior to shipment, and the other from the end-point distributors in taiwan, were studied. surface-sterilized kernels were plated onto two different agar media and the fungal colonies identified. high performance liquid chromatography was used to analyze mycotoxin levels. the predominant ear-rot fungi, in dec ... | 1994 | 7935737 |
| occurrence and toxicity of fusarium subglutinans from peruvian maize. | twenty-five samples of maize kernels collected at harvest time from geographically different corn fields in peru, were examined for the occurrence of toxigenic fusarium species. the most frequently recovered species were f. subglutinans (48%), f. moniliforme (46%), and f. equiseti (5%). other fusarium species isolated (up to 1%) included f. graminearum, f. acuminatum, f. solani, f. oxysporum, and f. culmorum. assays of fusarium culture extracts using artemia salina larvae, showed f. subglutinans ... | 1993 | 8413501 |
| occurrence of the mycotoxin moniliformin in maize (zea mays l.) ears infected by fusarium subglutinans (wollenw. & reinking) nelson et al. | fusarium subglutinans has been identified as a prevailing pathogen of maize ears in poland in the seasons 1985-1991. about 95-100% of ears with fusarium ear rot symptoms were infected by this species. moniliformin was present in all 57 ears with pink ear rot symptoms examined. fusarium-damaged kernels contained an average of 130.9 mg/kg of moniliformin, with large differences between individual samples each year--from 4.2 mg/kg to 530 mg/kg. | 1996 | 8718747 |
| fusaproliferin production by fusarium subglutinans and its toxicity to artemia salina, sf-9 insect cells, and iarc/lcl 171 human b lymphocytes. | fusarium subglutinans is an important pathogen of maize and other commodities worldwide. we examined mrc-115 and 71 other f. subglutinans strains from various geographic areas for their ability to synthesize fusaproliferin, a novel toxic sesterterpene recently isolated from f. proliferatum. fusaproliferin production ranged from 30 to 1,500 micrograms/g of dried ground substrate, with 33 strains producing more than 500 micrograms/g. in particular, strain mrc-115 produced as much as 1,100 to 1,300 ... | 1996 | 8795229 |
| ice nucleus production of fusarium moniliforme var. subglutinans in relation to its growth characteristics. | the effects of culture conditions on the ice nucleus production of fusarium moniliforme var. subglutinans isolated from the gut of larvae of the rice stem borer (chilo suppressalis walker) were examined. the ice nucleus production was only affected by cultivation temperature and ph: the optimum temperature and ph were 15 degrees c to 20 degrees c and 4.0 to 6.0, respectively. | 1996 | 8987607 |
| in-vitro antifungal susceptibility of clinical and environmental fusarium spp. strains. | the mics of amphotericin b, miconazole, ketoconazole, flucytosine, itraconazole and fluconazole for 19 isolates of fusarium oxysporum, 16 fusarium solani, seven fusarium verticilliodes, four fusarium proliferatum, four fusarium dimerum, three fusarium equiseti, and one each of the following species: fusarium graminearum, fusarium chlamydosporum, fusarium semitectum, fusarium avenaceum and fusarium subglutinans were determined by a broth microdilution method. thirty-eight of these isolates were o ... | 1997 | 9069536 |
| female fertility and mating type distribution in a south african population of fusarium subglutinans f. sp. pini | fusarium subglutinans f. sp. pini is the causal agent of pitch canker disease of pines. the initial occurrence of f. subglutinans f. sp. pini in south africa was limited to a single nursery, and isolates from this population are capable of reproducing sexually. we determined the effective population number, ne, of this population by using mating type and male/hermaphrodite polymorphisms as indicators. the effective population number for mating type, ne(mt), is 99% of the count (total population) ... | 1998 | 9603819 |
| biodegradation of anthracene by soil fungi. groupe pour l'etude du devenir des xénobiotiques dans l'environnement (gedexe). | a selection of 39 strains of micromycetes known as good degraders of polychlorinated aromatic compounds, mostly isolated from soil and belonging to various taxonomic groups, have been investigated for anthracene degradation. toxicity and consumption assays, first evaluated on solid media, have not shown any toxicity of anthracene (1-100 mg.l-1) towards fungi. degradation of anthracene (10 mg.l-1) was then investigated in a liquid synthetic medium for 4 days and evaluated by hplc. among the 39 st ... | 1998 | 9661278 |
| fusarium subglutinans f. sp. pini represents a distinct mating population in the gibberella fujikuroi species complex | fusarium strains in the gibberella fujikuroi species complex cause diseases on a variety of economically important plants. one of these diseases, pitch canker of pinus spp., is caused by strains identified as fusarium subglutinans f. sp. pini. fertile crosses were detected between f. subglutinans f. sp. pini strains from south africa, california, and florida. f. subglutinans f. sp. pini strains were not cross-fertile with the standard tester strains of six of the seven other mating populations o ... | 1999 | 10049883 |
| polyclonal antibodies against fusaproliferin. | fusaproliferin (fp), a toxic metabolite of the world-wide maize pathogens fusarium proliferatum and fusarium subglutinans, was recently found to be a natural contaminant of maize. its toxic activity on haematopoietic human cell lines and its teratogenic effects on chicken embryos has been recently proved. therefore a sensitive, rapid, and inexpensive screening test to detect fp in agricultural commodities is necessary to protect human health. fp-hemiglutarate conjugated to modified bovine serum ... | 1999 | 10349720 |
| differentiation of fusarium subglutinans f. sp. pini by histone gene sequence data. | fusarium subglutinans f. sp. pini (= f. circinatum) is a pathogen of pine and is one of eight mating populations (i.e., biological species) in the gibberella fujikuroi species complex. this species complex includes f. thapsinum, f. moniliforme (= f. verticillioides), f. nygamai, and f. proliferatum, as well as f. subglutinans associated with sugarcane, maize, mango, and pineapple. differentiating these forms of f. subglutinans usually requires pathogenicity tests, which are often time-consuming ... | 1999 | 10427026 |
| determination of the mycotoxin moniliformin in cultures of fusarium subglutinans and in naturally contaminated maize by high-performance liquid chromatography-atmospheric pressure chemical ionization mass spectrometry. | a lc-ms method employing triethylamine as ion-pairing reagent for the determination of moniliformin in culture material and naturally contaminated maize samples is described. mass spectrometric detection of moniliformin was accomplished following atmospheric pressure chemical ionization to yield the deprotonated molecular ion [m-h]- at m/z 97. the moniliformin response was found to be linear over the injected range 10 ng to 700 ng and a detection limit of 10 ng was attainable at a signal-to-nois ... | 1999 | 10427758 |
| determination of the fusarium mycotoxins, fusaproliferin and beauvericin by high-performance liquid chromatography-electrospray ionization mass spectrometry. | a method is described using lc-ms for the detection of the mycotoxins fusaproliferin (fus) and beauvericin (bea) in cultures of fusarium subglutinans and in naturally contaminated maize. protonated molecular ion signals for fus and bea were observed at m/z 445 and m/z 784, respectively. collision induced dissociation of the readily dehydrated protonated molecular ion of the sesterterpene fus (m/z 427) led to the loss of another water molecule (m/z 409) and acetic acid (m/z 385), while the cyclic ... | 1999 | 10551350 |
| the effects of cereal substrate and temperature on production of beauvericin, moniliformin and fusaproliferin by fusarium subglutinans item-1434. | one strain of fusarium subglutinans (item-1434) isolated from maize ear rot in poland was tested for the ability to synthesize moniliformin (mon), beauvericin (bea) and fusaproliferin (fp) on six cereal substrates (wheat, rye, barley, oat, maize and rice kernels) for 3 weeks at 25 degrees c and on rice at three different temperatures (20, 25 and 30 degrees c). most mon (497 micrograms/g) was produced on rice; most bea (704 micrograms/g) on wheat or rice, and most fp (422 micrograms/g) on rye. wh ... | 1999 | 10755127 |
| effects of temperature, incubation period and substrate on production of fusaproliferin by fusarium subglutinans item 2404. | the kinetics of the production of fusaproliferin by fusarium subglutinans item 2404 in maize and rice cultures was investigated at various incubation temperatures. the growth rate of f. subglutinans was highest at 20 degrees c and 25 degrees c in maize cultures and at 15 degrees c in rice cultures. although the growth rate was higher in rice than in maize, the maximal production of fusaproliferin was obtained in maize cultures, with a maximum yield (4309 microg g(-1)) at 20 degrees c for 6 weeks ... | 1999 | 10797639 |
| thermal stability of moniliformin at varying temperature, ph, and time in an aqueous environment. | moniliformin (mon) is a widely occurring mycotoxin, produced mainly by fusarium proliferatum and fusarium subglutinans in corn, that has been shown to be acutely toxic for various animal species and is a suspected cause of keshan disease in china. the effects of temperature (100, 125, and 150 degrees c) and ph (4, 7, and 9) on the stability of mon were determined in aqueous buffer solutions at processing times ranging from 10 to 60 min. the percentage of mon reduction was positively related to i ... | 2000 | 11079708 |
| fusarium species (section liseola) and its mycotoxins in maize harvested in northern argentina. | maize and maize products harvested in small fields and stored by farmers in northern argentina were assayed for fusarium and fumonisin and beauvericin contamination. fumonisins were present in six of the 18 samples. the levels of fumonisins ranged from 603 to 1888 ng/kg. fumonisin b3 (fb3) and beauvericin were not detected in the samples evaluated. fusarium subglutinans was one of the most prevalent species isolated. twenty-five strains of f. subglutinans isolated from maize kernels and belongin ... | 2001 | 11552751 |
| production of intracellular milk-clotting enzyme in submerged cultures of fusarium subglutinans. | fusarium subglutinans (wollenweber and reinking) nelson et al. was found to produce intracellular milk-clotting enzyme (mce) with good milk-clotting activity (mca). the crude activity of the produced enzyme was recorded as optimum at 55 degrees c and ph 4.5. the highest yield i.e. 78.43 su/mg dry biomass was obtained after 4 days of rotary shaking at 30 degrees c when the fermentation medium containes wheat flour 2%, glucose 1% and (nh4)2so4 0.1% with an initial ph value 6.0. under these conditi ... | 2001 | 11720308 |
| pathogen challenge, salicylic acid, and jasmonic acid regulate expression of chitinase gene homologs in pine. | to better understand the molecular regulation of defense responses in members of the genus pinus, we tested the expression of various chitinase homologs in response to pathogen-associated signals. pschi4, a putative extracellular class ii chitinase, was secreted into liquid medium by pine cells and was also secreted by transgenic tobacco cells that ectopically expressed pschi4. extracellular proteins of pine were separated by isoelectric focusing; pschi4 was not associated with fractions contain ... | 2002 | 12026177 |
| rapid assessment of antimould efficacies of pressure-treated southern pine. | a membrane-screening method was developed in conjunction with flow cytometric (fc) analysis for determining the efficacies of antimould pressure-treatment formulations for mould species of cosmetic significance on southern pine. fusarium subglutinans, aspergillus flavus, penicillium chrysogenum, and paecilomyces spp. were the predominant moulds colonizing surfaces of the variously treated pine stored in sealed plastic bags over 3- to 6-month periods. nylon membranes placed directly on pressure-t ... | 2002 | 12483480 |
| purification of fusaproliferin from cultures of fusarium subglutinans by preparative high-performance liquid chromatography. | thirty-six fusarium strains were grown on cracked yellow corn and evaluated for optimum fusaproliferin production, with fusarium subglutinans e-1583 producing the highest levels (1600 microg/g). three solvent systems were tested for extracting fusaproliferin from the cultures of f. subglutinans e-1583. methanol gave the highest fusaproliferin recovery, followed by methanol/1% aqueous nacl (55:45, v/v) and acetonitrile/methanol/h(2)o (16:3:1, v/v/v). hexane partitioning was effective in removing ... | 2003 | 12517099 |
| identification and enumeration of fusarium species in poultry feed mixtures from slovakia. | thirty-three samples of poultry mixed feeds collected in the region of nitra (slovakia) from august 2001-april 2002 were assayed for the incidence of fusarium link: fr. species. in tested samples, the total frequency of isolated fusaria was determined to be 48 % (16 samples) and their counts ranged from 0.2 x 10(2) to 2.4 x 10( 4 ) cfu per g of the sample on dichloran chloramphenicol peptone agar (dcpa). of the total amount of fusarium isolates (609), the highest part (i.e. 584 isolates) was rep ... | 2003 | 12852734 |
| species diversity of and toxin production by gibberella fujikuroi species complex strains isolated from native prairie grasses in kansas. | fusarium species from agricultural crops have been well studied with respect to toxin production and genetic diversity, while similar studies of communities from nonagricultural plants are much more limited. we examined 72 fusarium isolates from a native north american tallgrass prairie and found that gibberella intermedia (fusarium proliferatum), gibberella moniliformis (fusarium verticillioides), and gibberella konza (fusarium konzum) dominated. gibberella thapsina (fusarium thapsinum) and gib ... | 2004 | 15066820 |
| interaction of ozone exposure and fusarium subglutinans inoculation on growth and disease development of loblolly pine seedlings. | seedlings from ten half-sib families of loblolly pine (pinus taeda) were exposed in open-top chambers to carbon-filtered air (cf), non-filtered air (nf), or air amended with ozone to 1.7 or 2.5 times ambient. after 105 days of exposure, half the seedlings within each family were wounded but not inoculated and half were wounded and inoculated with the pitch canker fungus, fusarium subglutinans, to which five families were relatively resistant. after an additional 50 days of ozone treatment, seedl ... | 1994 | 15091722 |
| fusaproliferin, beauvericin and fumonisin production by different mating populations among the gibberella fujikuroi complex isolated from maize. | the production of fumonisins, fusaproliferin and beauvericin by gibberella fujikuroi different mating populations isolated from maize in argentina was evaluated. from 203 strains of fusarium verticillioides (g. fujikuroi mating population a), 193 were fumonisin producers. among members of mating population a, female fertile strains produced 20% more toxin than female sterile ones. among 78 fusarium proliferatum strains (g. fujikuroi mating population d) 65 produced fumonisins. the percentage of ... | 2004 | 15119352 |
| [study on gene polymorphism of fumonisin-producing fusarium verticillioides strains]. | to study the gene polymorphism and genetic variation of fumonisin-producing or non-producing fusarium verticillioides strains isolated from different geographical areas and different samples. | 2005 | 16329605 |
| maize ear rot and moniliformin contamination by cryptic species of fusarium subglutinans. | fusarium subglutinans causes maize ear rot and contaminates grain with the mycotoxin moniliformin. previous dna sequence analysis divided f. subglutinans from maize into two cryptic species, designated groups 1 and 2. here, it was determined whether the two groups differ in the agriculturally important traits of virulence on maize and moniliformin production in planta. thirty-seven strains from u.s. maize were assigned to groups 1 and 2 by dna sequence analysis. in field tests, all strains were ... | 2006 | 16968109 |
| complete genetic linkage maps from an interspecific cross between fusarium circinatum and fusarium subglutinans. | the gibberella fujikuroi complex includes many plant pathogens of agricultural crops and trees, all of which have anamorphs assigned to the genus fusarium. in this study, an interspecific hybrid cross between gibberella circinata and gibberella subglutinans was used to compile a genetic linkage map. a framework map was constructed using a total of 578 aflp markers together with the mating type (mat-1 and mat-2) genes and the histone (h3) gene. twelve major linkage groups were identified (n=12). ... | 2007 | 17418597 |
| [study on the comparison of rdna and its sequence of fumonisin-producing strains]. | to study the phylogenetic relationships of fumonisin-producing fusarium verticillioides isolates from china and gibberella fujikuroi species complex. | 2007 | 17424850 |
| cryptic subspecies and beauvericin production by fusarium subglutinans from europe. | fusarium subglutinans is a maize ear rot pathogen and producer of beauvericin and other mycotoxins. this species has recently been split into two major phylogenetic within-species groups based on rflp dna sequence polymorphisms identified in the histone h3 and beta-tubulin sequences. a pan european collection of the fungus originating mostly from maize was subjected to phylogenetic analysis by rflp grouping and to chemical analysis for beauvericin production. of the 62 isolates belonging to grou ... | 2008 | 18804303 |
| use of gus transformants of fusarium subglutinans for determining etiology of mango malformation disease. | abstract fusarium subglutinans has been associated with mango floral and vegetative malformation, although confusion exists regarding the etiology of the disease. a wild-type isolate of f. subglutinans causing mango malformation disease was transformed with the gus (beta-glucuronidase) reporter and hygromycin resistance genes. five stable transformants were isolated containing varying copy numbers at different integration sites. specific gus activity was quantified for the transformants, whereas ... | 1999 | 18944716 |
| dominance of group 2 and fusaproliferin production by fusarium subglutinans from iowa maize. | fusarium subglutinans (teleomorph gibberella subglutinans, member of the gibberella fujikuroi complex) is an important toxigenic pathogen of maize. recently, two cryptic species (groups 1 and 2) have been described within f. subglutinans, but little is known about the occurrence of the two groups in north america or their relative capacities to produce mycotoxins. in this study, 58 f. subglutinans strains from kernels of maize grown in iowa, usa, were evaluated for cryptic speciation and product ... | 2009 | 19680913 |
| cutaneous hyalohyphomycosis caused by fusarium subglutinans. | | 2010 | 20413373 |
| total synthesis, assignment of the absolute stereochemistry, and structure-activity relationship studies of subglutinols a and b. | immunosuppressive drugs are used to prevent rejection of transplanted organs and treat autoimmune diseases. clinically approved immunosuppressive drugs possess undesirable side effects, including acute neurological toxicity, chronic nephrotoxicity, and osteoporosis. as a result, considerable efforts have been devoted to the identification of immunosuppressive natural products that lack cytotoxicity and undesirable side effects on bone structure. subglutinols a (1 a) and b (1 b) are diterpene pyr ... | 2010 | 20564278 |
| gibberellin biosynthesis and gibberellin oxidase activities in fusarium sacchari, fusarium konzum and fusarium subglutinans strains. | several isolates of three fusarium species associated with the gibberella fujikuroi species complex were characterized for their ability to synthesize gibberellins (gas): fusarium sacchari (mating population b), fusarium konzum (mating population i) and fusarium subglutinans (mating population e). of these, f. sacchari is phylogenetically related to fusarium fujikuroi and is grouped in the asian clade of the complex, while f. konzum and f. subglutinans are only distantly related to fusarium fuji ... | 2010 | 20570295 |
| molecular characterization of fusarium subglutinans associated with mango malformation. | abstract mango malformation is a serious disease of mangifera indica in tropical and subtropical regions of the world. this disease is caused by fusarium subglutinans, which is also associated with diseases on many other hosts, such as pineapple, pine, maize and sugarcane. the f. subglutinans strains associated with different hosts are virtually indistinguishable using morphological characters, but can be easily differentiated using histone and beta-tubulin gene sequencing, and some have subsequ ... | 2000 | 20572965 |
| gibberella fujikuroi mating population e is associated with maize and teosinte. | summary isolates of fusarium subglutinans mating population e are usually found on maize. this fungus forms part of the so-called gibberella fujikuroi species complex. previously, f. subglutinans has been associated with two additional mating populations (b and h) and a variety of plant hosts. this was mainly due to a lack of diagnostic morphological characters, but the use of dna sequence information showed that the strains making up mating populations b, e and h, as well as those associated wi ... | 2001 | 20573009 |
| cell wall alterations in the leaves of fusariosis-resistant and susceptible pineapple cultivars. | fusariosis, caused by the fungus fusarium subglutinans f. sp. ananas (syn. f. guttiforme), is one of the main phytosanitary threats to pineapple (ananas comosus var. comosus). identification of plant cell responses to pathogens is important in understanding the plant-pathogen relationship and establishing strategies to improve and select resistant cultivars. studies of the structural properties and phenolic content of cell walls in resistant (vitoria) and susceptible (perola) pineapple cultivars ... | 2010 | 20607243 |
| a molecular based strategy for rapid diagnosis of toxigenic fusarium species associated to cereal grains from argentina. | fusarium species are worldwide causal agents of ear rot in cereals. their toxigenic potential is a health risk for both humans and animals. in argentina, most identification of these fungi has been based on morphological and cross-fertility criteria which are time consuming and require considerable expertise in fusarium taxonomy and physiology. dna based approaches have been reported as rapid, sensitive and specific alternatives to identify the main fumonisin and trichothecene-producing fusarium ... | 2010 | 20965064 |
| identification of new galactose oxidase genes in fusarium spp. | galactose oxidase (go) converts galactose to an aldehyde and has several biotechnological applications, including cancer diagnosis. it is mainly produced by fusarium austroamericanum but is also produced by fusarium acuminatum and by isolates of the fusarium graminearum and gibberella fujikuroi complexes. the f. austroamericanum go gaoa gene has been cloned, but the go genes from other secreting species have not been characterized. problems associated with the f. austroamericanum go such as high ... | 2010 | 21077113 |
| two new species of fusarium section liseola associated with mango malformation. | mango malformation is an economically important disease of mangifera indica globally. a recent dna-based study indicated that two distinct, phylogenetic lineages previously identified as fusarium subglutinans are associated with this disease in south africa. the objective of this study was to characterize fusarium isolates associated with mango malformation, including the two different f. subglutinans groups, based on morphological characteristics. for this purpose we examined fusarium strains i ... | 2002 | 21156544 |
| cryptic speciation in fusarium subglutinans. | fusarium isolates that form part of the gibberella fujikuroi species complex have been classified using either a morphological, biological, or phylogenetic species concept. problems with the taxonomy of fusarium species in this complex are mostly experienced when the morphological and biological species concepts are applied. the most consistent identifications are obtained with the phylogenetic species concept. results from recent studies have presented an example of discordance between the biol ... | 2002 | 21156574 |
| fusarium temperatum sp. nov. from maize, an emergent species closely related to fusarium subglutinans. | a large number of fusarium isolates closely related to f. subglutinans were collected from maize in belgium. we used a robust polyphasic approach to describe a new biological species, fusarium temperatum, within the gibberella fujikuroi species complex. f. temperatum can be distinguished from f. subglutinans and from other fusarium species within the gibberella fujikuroi species complex with aflp fingerprint profile, differences in the translation elongation factor 1-α and β-tubulin dna sequence ... | 2010 | 21186324 |
| fum cluster divergence in fumonisins-producing fusarium species. | fumonisins are polyketide-derived mycotoxins, produced by several fusarium species, and its biosynthetic pathway is controlled by the fum cluster--a group of genes exhibiting a common expression pattern during fumonisin biosynthesis. the most common are the b analogues with fumonisin b(1) (fb(1)) being the most prevalent. at least a part of the inter- and intraspecific variation in fbs synthesis level can be explained by the sequence differences inside fum cluster. the aim of our study was to ev ... | 2010 | 21315309 |
| ginsenoside f(1) production from ginsenoside rg(1) by a purified +¦-glucosidase from fusarium moniliforme var. subglutinans. | fusarium moniliforme var. subglutinans was selected from among 100 strains of fungi for producing ginsenoside f(1) from ginsenoside rg(1). the enzyme responsible was purified as a single 85-ákda band with a specific activity of 136-áu-ámg(-1). it hydrolysed glucose-linked ginsenosides rb(1), rd and rg(1) but not for other monosaccharide-linked ginsenosides, rb(2), rc, r(1), and re. under the optimum conditions of ph 6.0, 50-¦c, 30-áu-ál(-1) of enzyme, and 5-ámg rg(1)-áml(-1), 4-ámg f(1)-áml(-1) ... | 2011 | 21826401 |
| genetic analysis of growth, morphology and pathogenicity in the f(1) progeny of an interspecific cross between fusarium circinatum and fusarium subglutinans. | fusarium circinatum and fusarium subglutinans are two distinct species in the gibberella fujikuroi species complex. a genetic linkage map produced from an interspecific cross between these species was used to identify quantitative trait loci (qtls) associated with variation in mycelial growth and morphology of colony margins (cms) in the 94 f(1) progeny. mycelial growth was assessed by measuring culture size at 25°c and 30°c, while cm morphology was characterized in the parents and assessed in t ... | 2011 | 21872187 |
| Candida krusei and Kloeckera apis inhibit the causal agent of pineapple fusariosis, Fusarium guttiforme. | Studies based on microbial ecology and antagonistic interactions play an important role in the development of new alternative strategies in controlling plant pathogens and are relevant to further biotechnological applications. Antagonistic interactions between the yeasts Candida krusei and Kloeckera apis isolated from rotten pineapple fruits, and two isolates of the pathogenic filamentous fungus Fusarium guttiforme (Syn.: Fusarium subglutinans f. sp. ananas) resistant and susceptible to fungicid ... | 2011 | 22115444 |
| in vitro antifungal activity of terpinen-4-ol, eugenol, carvone, 1,8-cineole (eucalyptol) and thymol against mycotoxigenic plant pathogens. | the aim of this study was to examine the effect of five naturally occurring compounds from essential oils on 10 different species of mycotoxigenic fungi involved in several plant diseases. the antifungal activities of terpinen-4-ol, eugenol, carvone, 1,8-cineole (eucalyptol) and thymol were observed in vitro on fusarium subglutinans, fusarium cerealis, fusarium verticillioides, fusarium proliferatum, fusarium oxysporum, fusarium sporotrichioides, aspergillus tubingensis, aspergillus carbonarius, ... | 2011 | 22257275 |
| new pcr assays for the identification of fusarium verticillioides, fusarium subglutinans, and other species of the gibberella fujikuroi complex. | fusarium verticillioides and fusarium subglutinans are important fungal pathogens of maize and other cereals worldwide. in this study, we developed pcr-based protocols for the identification of these pathogens targeting the gaob gene, which codes for galactose oxidase. the designed primers recognized isolates of f. verticillioides and f. subglutinans that were obtained from maize seeds from several producing regions of brazil but did not recognize other fusarium spp. or other fungal genera that ... | 2011 | 22312242 |
| endophytic fungi from vitis labrusca l. ('niagara rosada') and its potential for the biological control of fusarium oxysporum. | we investigated the diversity of endophytic fungi found on grape (vitis labrusca cv. niagara rosada) leaves collected from salesópolis, sp, brazil. the fungi were isolated and characterized by amplified ribosomal dna restriction analysis, followed by sequencing of the its1-5.8s-its2 rdna. in addition, the ability of these endophytic fungi to inhibit the grapevine pathogen fusarium oxysporum f. sp herbemontis was determined in vitro. we also observed that the climatic factors, such as temperature ... | 2012 | 23315803 |
| production of enniatins a, a1, b, b1, b4, j1 by fusarium tricinctum in solid corn culture: structural analysis and effects on mitochondrial respiration. | enniatins (ens) are secondary fungal metabolites with hexadepsipeptidic chemical structure and they possess a number of potent biological activities that can contaminate several kind of food and foodstuffs increasing the exposure risk for consumers. ens are produced by several fusariun strains including fusarium subglutinans, fusarium proliferatum and fusarium tricinctum. production of a mixture of ens was performed by culturing f. tricinctum item 9496 on white corn as substrate. the solid cultu ... | 2012 | 23692767 |
| biological evaluation of subglutinol a as a novel immunosuppressive agent for inflammation intervention. | subglutinol a (1) is an immunosuppressive natural product isolated from fusarium subglutinans, an endophytic fungus from the vine tripterygium wilfordii. we show that 1 exerts multimodal immune-suppressive effects on activated t cells in vitro: subglutinol a (1) effectively blocks t cell proliferation and survival while profoundly inhibiting pro-inflammatory ifnγ and il-17 production by fully differentiated effector th1 and th17 cells. our data further reveal that 1 may exert its anti-inflammato ... | 2014 | 24900866 |
| antimicrobial activity and potential use of monoterpenes as tropical fruits preservatives. | banana, papaya and pineapple are the most consumed tropical fruits in the world, being brazil one of the main producers. fungi colletotrichum musae, colletotrichum gloeosporioides and fusarium subglutinans f.sp. ananas cause severe post harvest diseases and losses in fruits quality. the aim of this work was to evaluate the effectiveness of five monoterpenes to inhibit the mycelial growth and conidia germination of these three phytopathogens. the monoterpenes citral, citronellal, l-carvone, isopu ... | 2008 | 24031197 |
| fusarium subglutinans: a new eumycetoma agent. | eumycetoma is a chronic subcutaneous mycosis mainly caused by madurella spp. fusarium opportunistic infections in humans are often caused by fusarium solani and fusarium oxysporum. we report a case of eumycetoma by f. subglutinans, diagnosed by clinical aspect and culture, and confirmed by pcr sequencing. the patient was successfully treated with oral itraconazole. to our knowledge, this is the second report of human infection and the first case of mycetoma by fusarium subglutinans. | 2013 | 24432236 |
| structural variation and dynamics of the nuclear ribosomal intergenic spacer region in key members of the gibberella fujikuroi species complex. | the intergenic spacer (igs) region of the ribosomal dna was cloned and sequenced in eight species within the gibberella fujikuroi species complex with anamorphs in the genus fusarium, a group that includes the most relevant toxigenic species. dna sequence analyses revealed two categories of repeated elements: long repeats and short repeats of 125 and 8 bp, respectively. long repeats were present in two copies and were conserved in all the species analyzed, whereas different numbers of short repe ... | 2013 | 23706073 |
| a multiplex real-time pcr method using hybridization probes for the detection and the quantification of fusarium proliferatum, f. subglutinans, f. temperatum, and f. verticillioides. | maize contamination with fusarium species is one of the major sources of mycotoxins in food and feed derivates. in the present study, a lightcycler(®) real-time pcr method using hybridization probes was developed for the specific identification, detection, and quantification of fusarium proliferatum, fusarium subglutinans, fusarium temperatum, and fusarium verticillioides, four mycotoxin-producing pathogens of maize. primers and hybridization probes were designed to target the translation elonga ... | 2012 | 23063186 |
| near-infrared (nir) hyperspectral imaging and multivariate image analysis to study growth characteristics and differences between species and strains of members of the genus fusarium. | near-infrared (nir) hyperspectral imaging was used to study three strains of each of three fusarium spp. (fusarium subglutinans, fusarium proliferatum and fusarium verticillioides) inoculated on potato dextrose agar in petri dishes after either 72 or 96 h of incubation. multivariate image analysis was used for cleaning the images and for making principal component analysis (pca) score plots and score images and local partial least squares discriminant analysis (pls-da) models. the score images, ... | 2012 | 22903431 |
| mating populations of fusarium section liseola from rice, sugarcane and maize. | mating studies were conducted on 78 isolates of fusarium species section liseola from rice, sugarcane and maize. from the crosses with tester strains of gibberella fujikuroi species complex, 64.1% (50 out of 78 isolates) were cross-fertile with tester strains of mating populations a to e. the results of the mating studies showed that of the 50 isolates, 19 belonged to mating population a (gibberella moniliformis), 18 to mating population b (gibberella sacchari), 4 to mating population e (gibbere ... | 2011 | 24575220 |
| first report of fusarium subglutinans causing leaf spot disease on cymbidium orchids in korea. | in 2006~2010, leaf spot symptoms, that is, small, yellow spots that turned into dark brown-to-black lesions surrounded by a yellow halo, were observed on cymbidium spp. in gongju, taean, and gapyeong in korea. a fusarium species was continuously isolated from symptomatic leaves; in pathogenicity testing, isolates caused leaf spot symptoms consisting of sunken, dark brown lesions similar to the original ones. the causal pathogen was identified as fusarium subglutinans based on morphological and t ... | 2015 | 26539053 |
| subglutinol a, an immunosuppressive α-pyrone diterpenoid from fusarium subglutinans, acts as an estrogen receptor antagonist. | subglutinol a is an immunosuppressive α-pyrone diterpenoid isolated from fusarium subglutinans that exhibits osteogenic activity. several non-steroid mycotoxins isolated from various strains of fusarium fungi exhibit female steroid hormone activities. in this study, we characterized the estrogenic activity of subglutinol a (1). subglutinol a blocked the 17β-estradiol-induced activation of reporter plasmids and endogenous estrogen-responsive target genes in a dose-dependent manner and efficiently ... | 2015 | 25896764 |
| fusarium temperatum and fusarium subglutinans isolated from maize in argentina. | fusarium temperatum and fusarium subglutinans isolated from the northwest region (noa region) of argentina were characterized using a polyphasic approach based on morphological, biological and molecular markers. some interfertility between the species was observed. the phylogenetic analysis showed that the two species represented two clades strongly supported by bootstrap values. the toxigenic profile of the strains was also determined. f. temperatum strains were fusaproliferin and beauvericin p ... | 2015 | 25647244 |
| characterization of the maize stalk rot pathogens fusarium subglutinans and f. temperatum and the effect of fungicides on their mycelial growth and colony formation. | maize is a socioeconomically important crop in many countries. recently, a high incidence of stalk rot disease has been reported in several maize fields in gangwon province. in this report, we show that maize stalk rot is associated with the fungal pathogens fusarium subglutinans and f. temperatum. since no fungicides are available to control these pathogens on maize plants, we selected six fungicides (tebuconazole, difenoconazole, fluquinconazole, azoxystrobin, prochloraz and kresoxim-methyl) a ... | 2014 | 25506304 |
| infection of corn ears by fusarium spp. induces the emission of volatile sesquiterpenes. | infection of corn (zea mays l.) ears with fungal pathogens of the fusarium genus might result in yield losses and in the accumulation of mycotoxins. the aim of this study was to investigate whether volatile profiles could be used to identify fusarium-infected corn ears. the volatiles released by corn ears infected by fusarium graminearum, fusarium verticillioides, and fusarium subglutinans were studied. volatile emission was recorded at 24 days postinoculation (dpi) and in a time series (from 4 ... | 2014 | 24816267 |
| investigations on fusarium spp. and their mycotoxins causing fusarium ear rot of maize in kosovo. | after wheat, maize (zea mays l.) is the second most important cereal crop in kosovo and a major component of animal feed. the purpose of this study was to analyse the incidence and identity of the fusarium species isolated from naturally infected maize kernels in kosovo in 2009 and 2010, as well as the mycotoxin contamination. the disease incidence of fusarium ear rot (from 0.7% to 40% diseased ears) on maize in kosovo is high. the most frequently fusarium spp. identified on maize kernels were f ... | 2013 | 24779930 |
| mycelial fungi completely remediate di(2-ethylhexyl)phthalate, the hazardous plasticizer in pvc blood storage bag. | this pioneering work describes how simply, inexpensively and efficiently novel fungi utilize the alarming plasticizer, di(2-ethylhexyl)phthalate (dehp) blended in pvc blood storage bags (bb). in order to quantify total dehp (33.5%, w/w) present in bb, it was extracted using n-hexane and confirmed by gc-ms. three mycelial fungi, viz., aspergillus parasiticus, fusarium subglutinans and penicillium funiculosum isolated in our laboratory form heavily plastics-contaminated soil - either singly or in ... | 2012 | 22858127 |
| [impact of fusarium toxins on growth and slaughter performance of broilers and turkeys]. | in two broiler and two turkey trials the influence of fusarium toxins in maize on growth and slaughter performance, on residues of toxins in carcass and litters and blood parameters were investigated. in one broiler and turkey trial with naturally contaminated maize the main contaminants were deoxynivalenol (don), moniliformin (mon and beauvericin (bea). in one further broiler and turkey trial with inoculated maize (fusarium subglutinans) the main contaminants were mon and bea. the level of cont ... | 2003 | 23604774 |
| effects of beauvericin to mammalian tissue and its production by austrian isolates offusarium proliferatum and fusarium subglutinans. | austrian isolates offusarium subglutinans andfusarium proliferatum were studied for their ability to produce beauvericin, moniliformin and fumonisin b1 and b2 under laboratory conditions. analytical methodology for beauvericin was specially adapted for this task. our analyses showed that the strains produced beauvericin up to 687 mg /kg maize and moniliformin up to 70 mg/kg. the culture off. proliferatum in addition produced fumonisin b1 and b2 at levels of 106 and 61 mg/kg,respectively. the pre ... | 1997 | 23604733 |
| relationship of phenotypic and genetic variation in plantago lanceolata to disease caused by fusarium moniliforme var. subglutinans. | naturally established individuals of plantago lanceolata with the inflorescence disease caused by fusarium moniliforme var. subglutinans had more inflorescences and were more likely to be male-sterile than healthy plants. half-sib families planted in the field varied in the percentage of diseased plants, the number of inflorescences per plant, the incidence of male-sterility, and the pattern of inflorescence phenology. the rankings of families with respect to disease incidence was, however, not ... | 1984 | 28312115 |
| spatial patterns of disease induced by fusarium moniliforme var. subglutinans in a population of plantago lanceolata. | at low densities, plantago lanceolata was less likely to have the inflorescence disease caused by the fungus fusarium moniliforme var. subglutinans. where the disease was found, as host density increased, the proportion diseased remained constant or decreased. the pathogen's inability to exploit high host densities may be due to both the long disease cycle and the reduced efficiency of disease spread in heterogeneous habitats. diseased plants were randomly spaced early in the summer but were lat ... | 1984 | 28310753 |