| molecular cloning and nucleotide sequencing of the partial genomes of agropyron and hordeum mosaic viruses, two members of the rymovirus genus in the taxonomic family potyviridae. | complementary dnas encompassing the coat protein coding and adjacent regions of agropyron mosaic virus (agmv) and hordeum mosaic virus (homv) were cloned and sequenced. comparison with other sequenced potyviruses indicated that each clone contained the 3'-non-coding region (3'ncr), the coat protein (cp) gene and part of the nuclear inclusion protein (nib) gene. nucleotide and amino acid sequence comparisons of the 3'-terminal regions of these and other rymoviruses indicate that distinct groups e ... | 1996 | 8973527 |
| phylogenetic justification for splitting the rymovirus genus of the taxonomic family potyviridae. | the potyviridae family has been divided into four genera on the basis of vector transmission, as follows: potyvirus genus (aphid), rymovirus genus (mite), bymovirus genus (fungus) and ipomovirus genus (whitefly). however recent sequence comparisons of the coat protein and 3' ncr regions of the potyviruses have demonstrated that the rymoviruses appear to be a group of two unrelated clusters namely ryegrass mosaic virus (rgmv), agropyron mosaic virus (agmv) and hordeum mosaic virus (homv) in one g ... | 1996 | 8973537 |
| molecular cloning, sequencing, and phylogenetic relationships of a new potyvirus: sugarcane streak mosaic virus, and a reevaluation of the classification of the potyviridae. | the nucleic acid of a serologically distinct potyvirus, originally isolated out of sugar cane from pakistan, was reverse transcribed and the 3' terminal 2000 bp was pcr amplified, cloned, and sequenced. phylogenetic comparisons of viruses representing each genus of the potyviridae show that the pakistani isolate is most closely related to the rymoviruses wheat streak mosaic virus (wsmv) and brome streak mosaic virus. we therefore propose that this new virus species be named sugar cane streak mos ... | 1998 | 10051385 |
| a universal pcr primer to detect members of the potyviridae and its use to examine the taxonomic status of several members of the family. | a universal primer (sprimer: 5'-ggx aay aay agy ggx caz cc-3', x = a, g, c or t; y = t or c; z = a or g), designed from the consensus sequences that code for the conserved sequence gnnsgqp in the nib region of members of the family potyviridae, was used to amplify by rt-pcr the 3'-terminal genome regions from infected plant samples representing 21 different viruses in the family. sequencing of some of the fragments (c. 1.7 kb) showed that the type strain (attc pv-107) of oat necrotic mottle viru ... | 2001 | 11402861 |
| analyses of the complete sequence of the genome of wheat eqlid mosaic virus, a novel species in the genus tritimovirus. | the complete nucleotide sequence of the genome of wheat eqlid mosaic virus (weqmv) (excluding the poly a tail) comprised 9636 nucleotides including 5' and 3' noncoding regions of 137 and 172 nt, respectively. it contained a single orf coding for a polyprotein of 3,109 amino acid residues and had a deduced genome organization typical of members of the family potyviridae and with proteinase cleavage sites very similar to those of the members of the genus tritimovirus. pairwise and multiple alignme ... | 2008 | 18648923 |
| phylogenetic relationships within the family potyviridae: wheat streak mosaic virus and brome streak mosaic virus are not members of the genus rymovirus. | abstract the complete nucleotide sequence of wheat streak mosaic virus (wsmv) has been determined based on complementary dna clones derived from the 9,384-nucleotide (nt) rna of the virus. the genome of wsmv has a 130-nt 5' leader and 149-nt 3'-untranslated region and is polyadenylated at the 3' end. wsmv rna encodes a single polyprotein of 3,035 amino acid residues and has a deduced genome organization typical for a member of the family potyviridae (5'-p1/hc-pro/p3/6k1/ci/6k2/vpg-nia/nib/cp-3') ... | 1998 | 18944883 |
| complete nucleotide sequence of rose yellow mosaic virus, a novel member of the family potyviridae. | the complete genomic sequence of rose yellow mosaic virus (roymv) was determined and found to have all the features that are characteristic of members of the family potyviridae. the roymv genome is 9508 nucleotides long excluding the 3'-poly-(a) tail and contains a single open reading frame encoding a polyprotein of 3067 amino acids. the roymv p3 and ci cistrons are shorter than those of other members of the family potyviridae, and the 6k1 cistron is completely absent. comparative sequence analy ... | 2013 | 23553457 |
| eriophyid mite transmission and host range of a brome streak mosaic virus isolate derived from a full-length cdna clone. | biolistic inoculation of hordeum vulgare and phalaris paradoxa with a brome streak mosaic virus (brsmv) full-length cdna clone (pbrsmv(fl)) led to typical leaf streak symptoms in both plant species. infected h. vulgare plants showed a more stunted growth 8 weeks after symptom appearance compared to brsmv wild type (brsmv(wt))-infected plants. moreover, a slightly higher virus titer was observed in brsmv(fl)-inoculated h. vulgare, a. sativa and p. paradoxa plants. the biological activity of brsmv ... | 2008 | 17957328 |
| the complete nucleotide sequence and genome organization of the mite-transmitted brome streak mosaic rymovirus in comparison with those of potyviruses. | a virus isolate, designated as 11-cal, originating from southern france has been identified as an isolate of the mite-transmitted brome streak mosaic rymovirus (brsmv) by serological and morphological properties. brsmv is a member of the genus rymovirus of the family potyviridae. the complete nucleotide sequence of the rna genome of brsmv has been determined. the assembled rna is 9672 nucleotides in length, excluding a 3'-terminal poly(a)sequence. the rna contains one open reading frame (orf) of ... | 1995 | 7636484 |