| pneumocandins from zalerion arboricola. v. glutamic acid- and leucine-derived amino acids in pneumocandin a0 (l-671,329) and distinct origins of the substituted proline residues in pneumocandins a0 and b0. | | 1992 | 1362720 |
| pneumocandins from zalerion arboricola. i. discovery and isolation. | hplc bioautography of the directed biosynthesis of zalerion arboricola led to the discovery of pneumocandin b0 (l-688,786), a new antifungal and anti-pneumocystis carinii lipopeptide. isolation techniques were developed to separate this component from pneumocandin a0 (l-671,329) in fermentations of a mutant of zalerion arboricola. a number of related compounds were also isolated, which differ from pneumocandins a0 and b0 in the hydroxylation patterns on the ornithine, homotyrosine, and proline. | 1992 | 1490876 |
| pneumocandins from zalerion arboricola. ii. modification of product spectrum by mutation and medium manipulation. | zalerion arboricola atcc 20868 produces pneumocandin a0 (l-671,329), a cyclic hexapeptide with a dimethylmyristic acid side chain. this compound has anti-candida and anti-pneumocystis activities. we were interested in looking for other related compounds produced by this organism. to facilitate this search, a simple medium (s2) composed of d-mannitol, peptonized milk, lactic acid, glycine, kh2po4 and trace elements, which supported the production of a number of such compounds, was designed. for t ... | 1992 | 1490877 |
| pneumocandins from zalerion arboricola. iii. structure elucidation. | pneumocandin b0 (6) and six related lipopeptides are antifungal and anti-pneumocystis carinii agents from mutants of zalerion arboricola, whose structures were determined mainly on the basis of spectroscopic analysis. they belong, along with pneumocandin a0 (l-671,329) previously isolated from these laboratories, to the echinocandin class of antifungal agents. the product from base-catalyzed ring opening involving the hemiaminal position of the dihydroxyornithine residue of b0, has been clearly ... | 1992 | 1490878 |
| pneumocandins from zalerion arboricola. iv. biological evaluation of natural and semisynthetic pneumocandins for activity against pneumocystis carinii and candida species. | a series of lipopeptide compounds co-produced during the fermentation of pneumocandin a0 (l-671,329) and related semisynthetic compounds were evaluated in vivo against pneumocystis carinii pneumonia and systemic candidiasis. in addition, they were tested in vitro against a panel of pathogenic candida species and in a candida membrane 1,3-beta-d-glucan synthesis assay. the results of these studies demonstrate that pneumocandin a0 and pneumocandin b0 (l-688,786) are the most potent compounds when ... | 1992 | 1490879 |
| l-671,329, a new antifungal agent. i. fermentation and isolation. | in screening for new antifungal agents from fungi, a new lipopeptide antifungal agent, l-671,329, similar to echinocandin b, has been isolated from zalerion arboricola. studies indicate that l-671,329 is produced under both solid and liquid fermentation conditions. | 1989 | 2647703 |
| improvement in the titer of echinocandin-type antibiotics: a magnesium-limited medium supporting the biphasic production of pneumocandins a0 and b0. | we have developed a liquid fermentation medium for the submerged culture of the fungus, zalerion arboricola, which supports the rapid production of an echinocandin-type antibiotic, pneumocandin a0 (formerly l-671,329), in yields increased at least 4-fold over those reported previously. the improvements were achieved through medium simplification, substitution of high levels of mannitol for glycerol as the major source of carbon, and restriction of available magnesium. antibiotic formation in bat ... | 1993 | 7763443 |
| heterologous transformation of zalerion arboricola. | a heterologous dna-mediated transformation system was developed for the pneumocandin-producing fungus z. arboricola that was based on either conferral of hygromycin b resistance or complementation of a nitrate reductase mutant. hygromycin-resistant transformants were selected with plasmid pcsn43 which contains the e. coli hygromycin b phosphotransferase gene under the control of aspergillus nidulans trpc transcription signals. transformation frequencies were about four transformants per microgra ... | 1994 | 7859303 |
| pneumocandin d0, a new antifungal agent and potent inhibitor of pneumocystis carinii. | pneumocandin d0 (9), a new member of the echinocandin class of antifungal agents, has been isolated as a minor constituent from fermentation broths of the filamentous fungi zalerion arboricola (atcc 20957). the structure of 9 has been determined mainly on the basis of spectroscopic analysis and by comparison with published data for similar compounds. to date, pneumocandin d0 has been found to be the most potent inhibitor of pneumocystis carinii development in vivo within the natural-occurring ec ... | 1994 | 8071120 |
| isolation and sequence analysis of the cdna encoding delta 1-pyrroline-5-carboxylate reductase from zalerion arboricola. | a cdna encoding delta 1-pyrroline-5-carboxylate reductase (p5cr) was isolated from the pneumocandin (pmo)-producing fungus, zalerion arboricola (za), by complementation of a p5cr-deficient mutant (pro3) of saccharomyces cerevisiae (sc). the cloned cdna was placed under control of the sc galactokinase (gal1) promoter and restored p5cr activity to the pro3 mutant. sequence analysis revealed that the za p5cr-encoding cdna encodes an approx. 35 kda protein with substantial amino acid (aa) identity t ... | 1996 | 8654976 |
| cryptocandin, a potent antimycotic from the endophytic fungus cryptosporiopsis cf. quercina. | a unique lipopeptide antimycotic, termed cryptocandin, is described from cryptosporiopsis cf. quercina, an endophytic fungus. cryptocandin, with a molecular mass of 1079 da, contains equimolar amounts of 3,4-dihydroxyhomotyrosine, 4-hydroxyproline, threonine, glutamine, 3-hydroxy-4-hydroxymethylproline, 4,5-dihydroxyornithine and palmitic acid. cryptocandin is chemically related to well-known antimycotics, the echinocandins and pneumocandins, which are produced by such fungi as zalerion arborico ... | 1999 | 10463158 |