| a transposable partitioning locus used to stabilize plasmid-borne hydrogen oxidation and trifolitoxin production genes in a sinorhizobium strain. | improved nitrogen-fixing inoculum strains for leguminous crops must be able to effectively compete with indigenous strains for nodulation, enhance legume productivity compared to the productivity obtained with indigenous strains, and maintain stable expression of any added genes in the absence of selection pressure. we constructed a transposable element containing the tfx region for expression of increased nodulation competitiveness and the par locus for plasmid stability. the transposon was ins ... | 1998 | 9572932 |
| desulfurization of benzothiophene by the gram-negative bacterium, sinorhizobium sp. kt55. | sinorhizobium sp. kt55 was the first gram-negative isolate to be capable of utilizing benzothiophene as the sole source of sulfur. by gc-ms analysis of metabolites of benzothiophene by this strain, benzothiophene sulfone, benzo[e][1,2]oxathiin s-oxide and o-hydroxystyrene were detected, suggesting that the benzothiophene desulfurization pathway of this strain is benzothiophene-->benzothiophene sulfoxide-->benzothiophene sulfone-->benzo[e][1,2]oxathiin s-oxide-->o-hydroxystyrene. desulfurization ... | 2001 | 11400068 |
| evolutionary relationship of phototrophic bacteria in the alpha-proteobacteria based on farnesyl diphosphate synthase. | partial sequences of farnesyl diphosphate (fpp) synthase genes derived from the rhodobacter-rhodovulum group and from the rhodopseudomonas palustris-bradyrhizobium japonicum group of the alpha-proteobacteria were subjected to phylogenetic analysis to investigate the relationships of phototrophic and non-phototrophic bacteria in the alpha-proteobacteria . the four rhodovulum species formed a monophyletic group within the rhodobacter cluster, and agrobacterium ferrugineum iam 12616(t) intermingled ... | 2002 | 12055311 |
| symbiotic and taxonomic diversity of rhizobia isolated from acacia tortilis subsp. raddiana in africa. | a collection of rhizobia isolated from acacia tortilis subsp. raddiana from various sites in the north and south of sahara was analyzed for their diversity at both taxonomic and symbiotic levels. on the basis of whole cell protein (sds-page) and 16s rdna sequence analysis, most of the strains were found to belong to the sinorhizobium and mesorhizobium genera where they may represent several different genospecies. despite their chromosomal diversity, most a. tortilis mesorhizobium and sinorhizobi ... | 2002 | 12086180 |
| biodesulfurization of benzothiophene and dibenzothiophene by a newly isolated rhodococcus strain. | rhodococcus sp. kt462, which can grow on either benzothiophene (bt) or dibenzothiophene (dbt) as the sole source of sulfur, was newly isolated and characterized. gc and gc-ms analyses revealed that strain kt462 has the same bt desulfurization pathway as that reported for paenibacillus sp. a11-2 and sinorhizobium sp. kt55. the desulfurized product of dbt produced by this strain, as well as other dbt-desulfurizing bacteria such as r. erythropolis ka2-5-1 and r. erythropolis igts8, was 2-hydroxybip ... | 2002 | 12111165 |
| structural characterization of the lipid a component of sinorhizobium sp. ngr234 rough and smooth form lipopolysaccharide. demonstration that the distal amide-linked acyloxyacyl residue containing the long chain fatty acid is conserved in rhizobium and sinorhizobium sp. | a broad-host-range endosymbiont, sinorhizobium sp. ngr234 is a component of several legume-symbiont model systems; however, there is little structural information on the cell surface glycoconjugates. ngr234 cells in free-living culture produce a major rough lipopolysaccharide (lps, lacking o-chain) and a minor smooth lps (containing o-chain), and the structure of the lipid a components was investigated by chemical analyses, mass spectrometry, and nmr spectroscopy of the underivatized lipids a. t ... | 2003 | 12456672 |
| symbiotic conditions induce structural modifications of sinorhizobium sp. ngr234 surface polysaccharides. | when the rhizosphere is starved of nitrogen, the soil bacteria rhizobium are able to infect legume roots and invade root nodules, where they can fix atmospheric nitrogen. nod boxes, the nod gene promoters located on the rhizobial symbiotic plasmid, are activated by means of flavonoids present in the legume root exudates, leading to the synthesis of lipochitooligomers: the nod factors. several recent works pointed out the importance of rhizobial surface polysaccharides in establishing the highly ... | 2002 | 12460942 |
| identification of a third sulfate activation system in sinorhizobium sp. strain br816: the cysdn sulfate activation complex. | sinorhizobium sp. strain br816 possesses two nodpq copies, providing activated sulfate (3'-phosphoadenosine-5'-phosphosulfate [paps]) needed for the biosynthesis of sulfated nod factors. it was previously shown that the nod factors synthesized by a nodpq double mutant are not structurally different from those of the wild-type strain. in this study, we describe the characterization of a third sulfate activation locus. two open reading frames were fully characterized and displayed the highest simi ... | 2003 | 12676676 |
| low sequence similarity and gene content of symbiotic clusters of bradyrhizobium sp. wm9 (lupinus) indicate early divergence of "lupin" lineage in the genus bradyrhizobium. | two sequenced nodulation regions of lupin bradyrhizobium sp. wm9 carried the majority of genes involved in the nod factor production. the nod region i harbored: nola, nodd, noda, nodb, nodc, nods, nodi, nodj, nolo, nodz, fixr, nifa, fixa, nodm, nolk and noel. this gene arrangement resembled that found in the nodulation region of bradyrhizobium japonicum usda110, however strain wm9 harbored only one nodd gene copy, while the nodm, nolk and noel genes had no counterparts in the 410 kb symbiotic re ... | 2003 | 14533715 |
| capillary electrophoresis as a simple and sensitive method to study polysaccharides of sinorhizobium sp. ngr234. | we report the minimum amounts of polysaccharides (6.5 ng) that can be studied using capillary electrophoresis-laser-induced fluorescence (ce-lif) and describe the separation of the various monosaccharides susceptible to occur in sinorhizobium cell wall. on-gel hydrolysis of the polysaccharides is described. it allowed the easy and rapid determination of the monosaccharide composition of the excised lipopolysaccharides. the composition obtained fits with gas chromatography-mass spectrometry (gc-m ... | 2003 | 14595683 |
| effects of biotic and abiotic constraints on the symbiosis between rhizobia and the tropical leguminous trees acacia and prosopis. | n2-fixing, drought tolerant and multipurpose acacia and prosopis species are appropriate trees for reforestation of degraded areas in arid and semiarid regions of the tropics and subtropics. acacia and prosopis trees form n2-fixing nodules with a wide range of rhizobia, for example african acacias mainly with sinorhizobium sp. and mesorhizobium sp., and australian acacias with bradyrhizobium sp. although dry and hot seasons restrict formation of n2-fixing nodules on acacia and prosopis spp., ful ... | 2003 | 15242281 |
| production of amino acids by rhizobium, mesorhizobium and sinorhizobium strains in chemically defined media. | five strains of rhizobium spp, one strain of mesorhizobium loti and two strains of sinorhizobium meliloti were tested for their ability to grow in chemically-defined medium lacking growth factor. qualitative and quantitative production of aspartic acid, serine, glutamic acid, glycine, histidine, threonine, arginine, alanine, proline, cysteine, tyrosine, valine, methionine, lysine, isoleucine, leucine, and phenylalanine was determined by the use of mannitol as sole carbon source. strains of rhizo ... | 2004 | 15378411 |
| characterization of bdha, encoding the enzyme d-3-hydroxybutyrate dehydrogenase, from sinorhizobium sp. strain ngr234. | a genomic library of sinorhizobium sp. strain ngr234 was introduced into escherichia coli ls5218, a strain with a constitutively active pathway for acetoacetate degradation, and clones that confer the ability to utilize d-3-hydroxybutyrate as a sole carbon source were isolated. subcloning experiments identified a 2.3 kb ecori fragment that retained complementing ability, and an orf that appeared orthologous with known bdha genes was located within this fragment. the deduced ngr234 bdha amino aci ... | 2005 | 15621424 |
| combined application of triton x-100 and sinorhizobium sp. pb002 inoculum for the improvement of lead phytoextraction by brassica juncea in edta amended soil. | the process of edta-assisted lead phytoextraction from the bovisa (milan, italy) brownfield soil was optimized in microcosms vegetated with brassica juncea. an autochthonous plant growth-promoting rhizobacterium (pgpr), sinorhizobium sp. pb002, was isolated from the rhizosphere of b. juncea grown on the pb-contaminated soil in presence of 2 mm edta. the strain was augmented (10(8) cfu g(-1) soil) in vegetated microcosms to stimulate b. juncea biomass production and, hence, its phytoextraction po ... | 2006 | 16153689 |
| structural characterization of a flavonoid-inducible pseudomonas aeruginosa a-band-like o antigen of rhizobium sp. strain ngr234, required for the formation of nitrogen-fixing nodules. | rhizobium (sinorhizobium) sp. strain ngr234 contains three replicons, the smallest of which (pngr234a) carries most symbiotic genes, including those required for nodulation and lipo-chito-oligosaccharide (nod factor) biosynthesis. activation of nod gene expression depends on plant-derived flavonoids, nodd transcriptional activators, and nod box promoter elements. nod boxes nb6 and nb7 delimit six different types of genes, one of which (fixf) is essential for the formation of effective nodules on ... | 2005 | 16159781 |
| degradation pathways of phenanthrene by sinorhizobium sp. c4. | sinorhizobium sp. c4 was isolated from a polycyclic aromatic hydrocarbon (pah)-contaminated site in hilo, hi, usa. this isolate can utilize phenanthrene as a sole carbon source. sixteen metabolites of phenanthrene were isolated and identified, and the metabolic map was proposed. degradation of phenanthrene was initiated by dioxygenation on 1,2- and 3,4-c, where the 3,4-dioxygenation was dominant. subsequent accumulation of 5,6- and 7,8-benzocoumarins confirmed dioxygenation on multiple positions ... | 2006 | 16317542 |
| a histidine kinase sensor protein gene is necessary for induction of low ph tolerance in sinorhizobium sp. strain bl3. | the aim of this investigation was to identify and isolate genes involved in acid tolerance from sinorhizobium sp. strain bl3. it was hypothesized that acid tolerance of strain bl3 could be enhanced by high level expression of certain genes involved in acid tolerance, following insertion of these genes in a multiple copy plasmid. a cosmid clone library of bl3 was introduced into bl3, and the transconjugant colonies were selected at low ph. a single cosmid containing genes for acid tolerance was i ... | 2006 | 16341462 |
| recombination and selection shape the molecular diversity pattern of nitrogen-fixing sinorhizobium sp. associated to medicago. | we investigate the genetic structure and molecular selection pattern of a sympatric population of sinorhizobium meliloti and sinorhizobium medicae. these bacteria fix nitrogen in association with plants of the genus medicago. a set of 116 isolates were obtained from a soil sample, from root nodules of three groups of plants representing among-species, within-species and intraline diversity in the medicago genus. bacteria were characterized by sequencing at seven loci evenly distributed along the ... | 2006 | 16911196 |
| the alternative sigma factor rpoh2 is required for salt tolerance in sinorhizobium sp. strain bl3. | the objectives of this investigation were to isolate the rpoh2 gene encoding an alternative sigma factor from sinorhizobium sp. bl3 and to determine its role in exopolysaccharide (eps) synthesis, salt tolerance and symbiosis with phaseolus lathyroides. the rpoh2 gene of rhizobium sp. strain tal1145 is known to be required for eps synthesis and effective nodulation of leucaena leucocephala. three overlapping cosmid clones containing the rpoh2 gene of bl3 were isolated by complementing an rpoh2 mu ... | 2006 | 16934437 |
| inactivation of the nodh gene in sinorhizobium sp. br816 enhances symbiosis with phaseolus vulgaris l. | sulfate modification on rhizobium nod factor signaling molecules is not a prerequisite for successful symbiosis with the common bean (phaseolus vulgaris l.). however, many bean-nodulating rhizobia, including the broad host strain sinorhizobium sp. br816, produce sulfated nod factors. here, we show that the nodh gene, encoding a sulfotransferase, is responsible for the transfer of sulfate to the nod factor backbone in sinorhizobium sp. br816, as was shown for other rhizobia. interestingly, inacti ... | 2007 | 17233732 |
| detection and organization of atrazine-degrading genetic potential of seventeen bacterial isolates belonging to divergent taxa indicate a recent common origin of their catabolic functions. | a collection of 17 atrazine-degrading bacteria isolated from soils was studied to determine the composition of the atrazine-degrading genetic potential (i.e. trzn, trzd and atz) and the presence of is1071. the characterization of seven new atrazine-degrading bacteria revealed for the first time the trzn-atzbc gene composition in gram-negative bacteria such as sinorhizobium sp. or polaromonas sp. three main atrazine-degrading gene combinations (i) trzn-atzbc, (ii) atzabc-trzd and (iii) atzabcdef ... | 2007 | 17561946 |
| toxic effects of arsenic on sinorhizobium-medicago sativa symbiotic interaction. | recently, the rhizobium-legume symbiotic interaction has been proposed as an interesting tool in bioremediation. however, little is known about the effect of most common contaminants on this process. the phytotoxic effects of arsenic on nodulation of medicago sativa have been examined in vitro using the highly arsenic resistant and symbiotically effective sinorhizobium sp. strain ma11. the bacteria were able to grow on plates containing as concentrations as high as 10 mm. nevertheless, as little ... | 2008 | 18039553 |
| functional nodfe genes are present in sinorhizobium sp. strain mus10, a symbiont of the tropical legume sesbania rostrata. | we have cloned the nodfe operon from sinorhizobium sp. strain mus10. mus10 nodf shows sequence homology to acyl carrier protein and enables an s. meliloti nodf mutant to effectively nodulate alfalfa. our results demonstrate the occurrence of nodfe in a symbiont that nodulates a legume host not belonging to the galegoid group. | 2008 | 18326678 |
| phylogenetic and symbiotic characterization of rhizobial bacteria nodulating argyrolobium uniflorum in tunisian arid soils. | forty-two bacterial isolates from root nodules of argyrolobium uniflorum growing in the arid areas of tunisia were characterized by phenotypic features, rflp, and sequencing of pcr-amplified 16s rrna genes. the isolates were found to be phenotypically diverse. the majority of the isolates tolerated 3% nacl and grew at temperatures up to 40 degrees c. phylogenetically, the new isolates were grouped in the genera sinorhizobium (27), rhizobium (13), and agrobacterium (2). except for the 2 agrobacte ... | 2008 | 18388992 |
| the cloned 1-aminocyclopropane-1-carboxylate (acc) deaminase gene from sinorhizobium sp. strain bl3 in rhizobium sp. strain tal1145 promotes nodulation and growth of leucaena leucocephala. | the objective of this study was to determine the role of 1-aminocyclopropane-1-carboxylate (acc) deaminase of symbionts in nodulation and growth of leucaena leucocephala. the acds genes encoding acc deaminase were cloned from rhizobium sp. strain tal1145 and sinorhizobium sp. bl3 in multicopy plasmids, and transferred to tal1145. the bl3-acds gene greatly enhanced acc deaminase activity in tal1145 compared to the native acds gene. the transconjugants of tal1145 containing the native or bl3 acds ... | 2008 | 18406559 |
| complexity of ultrafiltration membrane fouling caused by macromolecular dissolved organic compounds in secondary effluents. | recent investigations indicate the relevance of extracellular polymeric substances (eps) in terms of fouling of low-pressure membranes in advanced wastewater treatment. in this study, the high impact of the macromolecular fraction of effluent organic matter on fouling was confirmed in cross-flow ultrafiltration experiments using secondary effluent with and without autochthonous biopolymers. a method for the extraction of a natural mixture of eps derived from the bacterium sinorhizobium sp. is pr ... | 2008 | 18423516 |
| effects of medicago truncatula genetic diversity, rhizobial competition, and strain effectiveness on the diversity of a natural sinorhizobium species community. | we investigated the genetic diversity and symbiotic efficiency of 223 sinorhizobium sp. isolates sampled from a single mediterranean soil and trapped with four medicago truncatula lines. dna molecular polymorphism was estimated by capillary electrophoresis-single-stranded conformation polymorphism and restriction fragment length polymorphism on five loci (igs(nod), typa, virb11, avhb11, and the 16s rrna gene). more than 90% of the rhizobia isolated belonged to the sinorhizobium medicae species ( ... | 2008 | 18658290 |
| comparative metabolomic analysis of sinorhizobium sp. c4 during the degradation of phenanthrene. | comparative metabolic responses of sinorhizobium sp. c4 were investigated. comprehensive metabolites profiles, including polar metabolites, fatty acids, and polyhydroxyalkanoates were evaluated through untargeted metabolome analyses. intracellular metabolomes during the degradation of phenanthrene were compared with those from natural carbon sources. principal component analysis showed a clear separation of metabolomes of phenanthrene degradation from other carbon sources. shift to more hydropho ... | 2008 | 18668240 |
| [recombinant expression of an autoinducer synthase of sinorhizobium sp.1128 in escherichia coli]. | to explore the roles of quorum sensing system in establishing symbiosis between bacterium sinorhizobium sp.1128 and its plant host melilotus suaveolens ledeb. | 2008 | 19160810 |
| phenotypic, genomic and phylogenetic characteristics of rhizobia isolated from root nodules of robinia pseudoacacia (black locust) growing in poland and japan. | rhizobial strains, rescued from the root nodules of robinia pseudoacacia growing in japan and poland, were characterized for the phenotypic properties, genomic diversity as well as phylogeny and compared with the reference strains representing different species and genera of nodule bacteria. they had a moderately slow growth rate, a low tolerance to antibiotics, a moderate resistance to nacl and produced acid in yeast mannitol agar. cluster analysis based on the phenotypic features divided all b ... | 2009 | 19669127 |
| an apigenin-induced decrease in k-antigen production by sinorhizobium sp. ngr234 is y4gm- and nodd1-dependent. | cultured cells of sinorhizobium sp. ngr234 produce an abundance of capsular polysaccharides, or k antigens; however, cells that are cultured in the presence of apigenin, a nod gene inducer, exhibited a significant reduction in k-antigen production. the flavonoid-induced modulation in capsule production appeared to be related to the phase-shift changes associated with bacteroid differentiation. therefore, the polysaccharides were extracted from sinorhizobium sp. ngr234 bacteroids recovered from v ... | 2009 | 19679303 |
| identification of salt-tolerant sinorhizobium sp. strain bl3 membrane proteins based on proteomics. | sinorhizobium sp. bl3 is a salt-tolerant strain that can fix atmospheric nitrogen in symbiosis with leguminous host plants under salt-stress conditions. since cell membranes are the first barrier to environmental change, it is interesting to explore the membrane proteins within this protective barrier under salt stress. the protein contents of membrane-enriched fractions obtained from bl3 were analyzed by nanoflow liquid chromatography interfaced with electrospray ionization tandem mass spectrom ... | 2010 | 21576882 |
| multiple loci are involved in quorum quenching of autoinducer i molecules in the nitrogen-fixing symbiont (sino-) rhizobium sp. ngr234. | rhizobium sp. ngr234 is a unique α-proteobacterium (order - rhizobiales) that forms nitrogen-fixing nodules with more legumes than any other micro-symbiont. since we have previously described the complete genome sequence of ngr234, we now report on a genome wide functional analysis of the genes and enzymes involved in autoinducer i hydrolysis in this microbe. altogether we identified five cosmid clones that repeatedly gave a positive result in our function-based approach for the detection of aut ... | 2011 | 21642401 |
| biodegradation of geosmin in drinking water by novel bacteria isolated from biologically active carbon. | three strains of gram-negative bacteria capable of removing geosmin from drinking water were isolated from biologically active carbon and identified to be chryseobacterium sp., sinorhizobium sp. and stenotrophomonas sp. based on physio-biochemistry analysis and 16s rrna gene sequence analysis. removal efficiencies of 2 mg/l geosmin in mineral salts medium were 84.0%, 80.2% and 74.4% for chryseobacterium sp., sinorhizobium sp. and stenotrophomonas sp., respectively, while removal efficiencies of ... | 2011 | 21790055 |
| [obtaining of fluorescent-labeled nodule bacteria strains of wild legumes for their detection in vive and in vitro]. | a series of expression vectors containing genes of fluorescent proteins turbogfp and turborfp under the phage t5 constitutive promoter regulation, intended for lifetime marking of nodule bacteria is created: a series of vectors based on a broad-host-range replicon bbri, for marking strains with an expression of reporter gene from a transformed plasmid and a series of vectors based on a plasmid prl765gfp for marking strains by introduction genes of fluorescent proteins in a bacterial chromosome. ... | 2011 | 22295568 |
| highly enantioselective oxidation of α-hydroxyacids bearing a substituent with an aryl group: co-production of optically active α-hydroxyacids and α-ketoacids. | a novel and simple methodology for co-obtaining of enantiomerically pure α-hydroxyacids and α-ketoacids was developed by enantioselective oxidation of α-hydroxyacids bearing a substituent with an aryl group using α-hydroxyacid dehydrogenase (α-hadh). a high-throughput method was firstly established for screening of enantioselective α-hadhs. sinorhizobium sp. zjb1 101 with high activity and excellent enantioselectivity of α-hadh for oxidation of α-hydroxyacids bearing a substituent with an aryl g ... | 2012 | 23273376 |