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inducible antibacterial defence in the plant parasitic nematode meloidogyne artiellia.animals and plants both respond rapidly to pathogens by inducing the expression of defence-related genes. within this context, a prominent role has been assigned to the lysozyme. in the present study we isolated and carried out detailed analysis of the lysozyme gene in the plant nematode meloidogyne artiellia. the expression of lysozyme was up-regulated following exposure of m. artiellia juveniles to the gram-negative bacterium serratia marcescens. on the other hand, when isolated eggs containin ...200817950293
interactions between meloidogyne artiellia, the cereal and legume root-knot nematode, and fusarium oxysporum f. sp. ciceris race 5 in chickpea.abstract in the mediterranean basin, fusarium oxysporum f. sp. ciceris and the root-knot nematode meloidogyne artiellia coinfect chickpea. the influence of root infection (after inoculation with 20 nematode eggs and second-stage juveniles per gram of soil) by two m. artiellia populations, from italy and syria, on the reaction of chickpea lines and cultivars with partial resistance to fusarium wilt (ca 252.10.1.om, ca 255.2.5.0, cps 1, and pv 61) and with complete resistance to f. oxysporum f. sp ...200318943615
infection by meloidogyne artiellia does not break down resistance to races 0, 1a, and 2 of fusarium oxysporum f. sp. ciceris in chickpea genotypes.fusarium oxysporum f. sp. ciceris, and the root-knot nematode meloidogyne artiellia, coinfect chickpea crops in several countries of the mediterranean basin. the influence of root infection by m. artiellia on the reactions of chickpea genotypes with different reaction to infection with f. oxysporum f. sp. ciceris races 0, 1a, and 2 was investigated under controlled environmental conditions. results demonstrated that co-infection of chickpea genotypes resistant to specific fungal races by m. arti ...200818944296
a proteomic study of in-root interactions between chickpea pathogens: the root-knot nematode meloidogyne artiellia and the soil-borne fungus fusarium oxysporum f. sp. ciceris race 5.fusarium wilt caused by the fungus fusarium oxysporum f. sp. ciceris (foc) is the main soil-borne disease limiting chickpea production. management of this disease is achieved mainly by the use of resistant cultivars. however, co-infection of a foc-resistant plant by the fungus and the root-knot nematode meloidogyne artiellia (ma) causes breakdown of the resistance and thus limits its efficacy in the control of fusarium wilt. in this work we aimed to reveal key aspects of chickpea:foc:ma interact ...201121640211
characterization of the heat shock protein 90 gene in the plant parasitic nematode meloidogyne artiellia and its expression as related to different developmental stages and temperature.the full-length cdna and the corresponding gene of the heat shock protein 90, mt-hsp90, were isolated and characterized in the plant parasitic nematode meloidogyne artiellia. the full-length mt-hsp90 cdna contained a 5' untranslated region (utr) of 45 bp with the 22 bp trans-spliced leader sl1, an orf of 2172 bp encoding a polypeptide of 723 amino acids and a 3' utr of 191 bp. the deduced amino acid sequence of mt-hsp90 showed high similarity with other known hsp90s. five conserved amino acid si ...200919348876
the expression of the homologue of the caenorhabditis elegans lin-45 raf is regulated in the motile stages of the plant parasitic nematode meloidogyne artiellia.the ras-mapk signal transduction pathway controls multiple developmental events and is involved in the processing of olfactory information in the free living nematode caenorhabditis elegans. we have studied the ras-mapk pathway in the plant parasitic nematode meloidogyne artiellia. the genes mt-let-60, mt-lin-45, mt-mek-2 and mt-mpk-1 have been isolated and sequenced. each of them shows a high level of sequence similarity to its presumed ortholog in c. elegans and key functional domains are stru ...200616737746
analysis of chitin synthase function in a plant parasitic nematode, meloidogyne artiellia, using rnai.a single chitin synthase gene is responsible for chitin production in the eggshells of the plant parasitic nematode meloidogyne artiellia. in this paper we describe a functional analysis of this gene using rnai as well as further analysis of two similar genes from the free-living nematode caenorhabditis elegans. the parasitic life-style of m. artiellia required the development of a novel method for delivery of dsrna to nematode eggs that may be of utility in other experimental systems. c. elegan ...200515777697
molecular dissection of the rdna array and of the 5s rdna gene in meloidogyne artiellia: phylogenetic and diagnostic implications.the sequence of a 13.423 nucleotide genomic fragment has been determined for the plant parasitic nematode meloidogyne artiellia. it contains an entire rdna cluster, the bordering intergenic regions and portions of the flanking coding regions. the sequence analysis of the rdna repeats suggests homogeneity in m. artiellia, thus providing a further indication of the usefulness of these genes for the diagnostic identification of this species. the comparison of the secondary structures of the interna ...200415135452
structural and evolutionary analysis of the ribosomal genes of the parasitic nematode meloidogyne artiellia suggests its ancient origin. 200612387855
characterization of the (gaaa) microsatellite region in the plant parasitic nematode meloidogyne artiellia.microsatellites have become one of the most powerful genetic markers in biology. we have used dna sequencing to characterize a highly variable microsatellite (gaaa) locus in the root-knot nematode meloidogyne artiellia. the use of microsatellite flanking primers produced four amplification products that are defined as electromorphs, based on conventional length criteria. the sequencing of these four amplification products revealed the presence of new variants in the population due to sequence va ...200212137957
horizontal transfer of a bacterial gene involved in polyglutamate biosynthesis to the plant-parasitic nematode meloidogyne artiellia.analysis of a genomic fragment from the plant parasitic nematode meloidogyne artiellia revealed the presence of a gene which, in bacteria, is involved in the formation of polyglutamate capsule. searching of various databases, including the caenorhabditis elegans genome sequence and the large est datasets from a variety of parasitic nematodes, showed that no similar genes have been identified in other nematodes or in any other eukaryotic organisms. the m. artiellia gene has a typical eukaryotic s ...200111728474
nematode chitin synthases: gene structure, expression and function in caenorhabditis elegans and the plant parasitic nematode meloidogyne artiellia.although the presence of chitin in nematodes is well documented little is known about its synthesis in this phyletic group. the recently completed genome sequence of caenorhabditis elegans predicts two sequences with homology to chitin synthases (chitin-udp acetyl-glucosaminyl transferase; ec 2.4.1.16). we show that these genes are differentially expressed in a pattern that may reflect different functional roles. one gene is expressed predominantly in the adult hermaphrodite (the main egg-produc ...200111589574
modulation of expression at the level of splicing of cut-1 rna in the infective second-stage juvenile of the plant parasitic nematode meloidogyne artiellia.the cut-1 gene coding for cuticlin-1 has been isolated from the plant parasitic nematode meloidogyne artiellia. the sequence of the cut-1 gene was compared with the corresponding sequence from the free-living nematode caenorhabditis elegans. the high degree of similarity between the amino acid sequences, together with the occurrence of characteristic sequence motifs, indicates that the cuticlin-1 is a non-collagenous component of the cuticle also in plant parasitic nematodes. studies on the expr ...19979065692
a silent trans-splicing signal in the cuticlin-encoding gene of the plant-parasitic nematode meloidogyne artiellia.a gene (cut-1) coding for a cuticular protein, cuticlin-1, has been isolated and sequenced in the plant parasitic nematode, meloidogyne artiellia. the nucleotide sequence revealed a typical eukaryotic-like organization, exons and introns bordered by canonical sequences. the 5' flanking region presents nematode-specific sequence motifis, including a trans-splicing signal. studies on the expression of this gene demonstrated that, while in the adult females cut-1 is not expressed, the removal of th ...19968666256
a british root-knot nematode, meloidogyne artiellia n.sp. 196113701441
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