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induced robertsonian fusions and tandem translocations in mammalian cell cultures.cultures of a cattle cell line and a peromyscus eremicus cell line recovering from a pulse-treatment with mitomycin c, actinomycin d, 33258 hoechst, and nitrosoguanidine exhibited translocations between chromosomes at the centromeric regions (robertsonian fusions) as well as between centromere and telomere and between telomeres (tandem translocations). the frequency of robertsonian fusions was found to be dose-dependent and duration-dependent with the mitomycin treatment. biarmed chromosomes res ...197877213
studies in heterochromatin dna: accessibility of late replicating heterochromatin dna in chromatin to micrococcal nuclease digestion.heterochromatin dna in cactus mouse (peromyscus eremicus) replicates in the late s phase of cell cycle. a method of obtaining cells which contain dna preferentially labeled at heterochromatic areas by a pulse-labeling of late replicating dna is described. when the nuclei of p. eremicus cells containing radioactively labeled dna in heterochromatin were digested with micrococcal nuclease and the resultant nucleosomal dna was separated by gel electrophoresis, it was found that the repeat length of ...1979428268
distribution of 18+28s ribosomal genes in mammalian genomes.in situ hybridization with 3h 18s and 28s ribosomal rna from xenopus laevis has been used to study the distribution of dna sequences coding for these rnas (the nucleolus organizing regions) in the genomes of six mammals. several patterns of distribution have been found: 1) a single major site (rat kangaroo, seba's fruit bat), 2) two major sites (indian muntjac), 3) multiple sites in centromeric heterochromatin (field vole), 4) multiple sites in heterochromatic short arms (peromyscus eremicus), 5 ...19751104290
new host and locality records of coccidia (apicomplexa: eimeriidae) from rodents in the southwestern and western united states.one hundred forty-seven murid and heteromyid rodents were collected from various sites in the southwestern and western united states (arizona, colorado, new mexico, texas, and utah) and baja california norte, mexico, and their feces were examined for coccidial parasites. of these, 53 (36%) were infected with at least 1 coccidian; 45 of 53 (85%) of the infected rodents harbored only 1 species of coccidian. infected rodents included: 10 of 22 (45%) neotoma albigula, 3 of 11 (27%) neotoma floridana ...19911779282
eimeria species (apicomplexa: eimeriidae) infecting peromyscus rodents in the southwestern united states and northern mexico with description of a new species.of 198 deermice (peromyscus spp) collected from various localities in the southwestern united states and northern mexico, 106 (54%) had eimerian oocysts in their feces when examined. these included 50 of 106 (47%) peromyscus truei, 34 of 54 (63%) peromyscus maniculatus, 4 of 17 (24%) peromyscus leucopus, and 18 of 21 (86%) peromyscus eremicus. the following eimeria were identified from infected mice: eimeria arizonensis and eimeria langebarteli from p. truei; e. arizonensis, eimeria peromysci, a ...19854057004
comparative developmental osteology in three species of desert rodents, peromyscus eremicus, perognathus intermedius, and dipodomys merriami. 19734744935
aestivation in the cactus mouse, peromyscus eremicus. 19655865202
c-banding of peromyscus constitutive heterochromatin persists following histone hyperacetylation.approx. 35% of the dna of cultured cells from the cactus mouse, peromuscus eremicus, is contained in highly condensed constitutive heterochromatin which can be visualized in metaphase chromosomes stained by the c-band technique. previous studies have shown this constitutive heterochromatin to contain a large proportion of underacetylated, arginine-rich histones, the majority of which can be hyperacetylated when cells are treated with butyrate. in order to determine whether this simulation of the ...19836617770
investigation of the determinants of nuclear pore number.to assess the functional significance of nuclear pore complexes, we have investigated whether the number of pores per nucleus is determined by such factors as the nuclear volume, nuclear surface area, dna content, or aspects of nuclear activity. comparisons were made between cell types chosen to permit observation of differences in nuclear pore number as a function of differences in the other qualities measured. the number of nuclear pores was determined by freeze-etching and measurements of nuc ...19806966999
analysis of a brdu-sensitive site in the cactus mouse (peromyscus eremicus): chromosomal breakage and sister-chromatid exchange.when the thymidine analog brdu was incorporated into the dna of a fibroblast cell line derived from the cactus mouse peromyscus eremicus, a chromosome region with an increased frequency of gaps and breaks was observed. nearly a third of the chromatid aberrations found at this site were associated with a sister-chromatid exchange (sce) although this chromosome region showed no increase in sister-chromatid exchange in the absence of a gap or break. sces were significantly decreased in the remainde ...19807371461
susceptibility of heterochromatin to aphidicolin-induced chromosomal breakage.the distribution of aphidicolin-induced chromosomal lesions was analyzed to determine the relative breakage susceptibility of euchromatin and heterochromatin in the cactus mouse, peromyscus eremicus. the observed breakage was tested against expected distributions corresponding to the karyotypic proportions of autosomal euchromatin, autosomal heterochromatin, x-chromosomal euchromatin, and x-chromosomal heterochromatin. the distribution of induced breakage was independent of sex but dependent on ...19958529996
amplification of telomeric dna and the extent of karyotypic evolution.the distribution of telomeric dna in the genomes of the antelope ground squirrel, ammospermophilus harrisii (family sciuridae; 2n = 32) and the african black-footed cat, felis nigripes (family felidae; 2n = 38) were compared by fluorescence in situ hybridization (fish) technique. these two mammalian species have the highest and the lowest amount of c-banded regions, respectively. fish preparations with the human telomeric dna probe showed that all c-banded segments in the a. harrisii chromosomes ...19989779588
dermacentor hunteri (acari: ixodidae): seasonal variation in questing adults and on-host juvenile stages, and host associations and feeding behavior of larvae and nymphs.dermacentor hunteri bishopp is the only completely desert adapted tick in the nearctic realm, and chiefly parasitizes desert bighorn sheep (ovis canadensis shaw) as an adult. the remainder of its life history has been unknown. we conducted field investigations in the sonoran desert of the temporal and spatial variation of adult host-seeking ticks and of the host associations of juvenile ticks. additionally, the feeding success of juvenile ticks was assessed in the laboratory. adult ticks were fo ...19989835699
hantavirus (bunyaviridae) infections in rodents from orange and san diego counties, california.during a screening program to determine the extent of hantavirus activity in orange and san diego counties, california, serum samples from 2,365 rodents representing nine genera and 15 species were tested for hantavirus antibodies. a reverse transcription-polymerase chain reaction on selected seropositive rodents was used to identify the specific hantavirus. rodents positive for sin nombre virus (snv) antibodies by western blot included 86 (9.1%) of 948 deer mice (peromyscus maniculatus), four ( ...19999988327
comparative phylogeography of baileys' pocket mouse (chaetodipus baileyi) and the peromyscus eremicus species group: historical vicariance of the baja california peninsular desert.phylogenetic analysis of 699 bp of the mitochondrial dna (mtdna) coiii and 450 bp of the cytochrome b genes among 14 species of coarse-haired pocket mice (heteromyidae: chaetodipus) corroborated previous indications that genetic divergence between species and species groups within the genus is generally very high, suggesting old times of divergence, and that the nominal species c. baileyi represents a highly divergent lineage within the genus, with no closely related extant sister species. analy ...200011083931
arenavirus antibody in rodents indigenous to coastal southern california.the purpose of this study was to extend our knowledge on the geographic and natural rodent host ranges of new world arenaviruses in california. sera from 1,094 sigmodontine and 112 murine rodents were tested for antibody against whitewater arroyo and amapari viruses. antibody was found in 55 (4.6%) of the 1,206 rodents: 4 from northwestern san diego county, 3 from los angeles county, and 48 from orange county. the antibody-positive rodents included 8 (7.8%) of 103 neotoma fuscipes, 1 (0.6%) of 1 ...200011289675
social structure influences effects of pair-housing on wound healing.chronic stress or noxious stimuli delay wound healing in humans and rodents. the effects of stress on wound healing appear to be mediated by the hypothalamic-pituitary-adrenal (hpa) axis and, in particular, increases in corticosteroids. as previously shown, positive social interaction faciltiates wound healing through suppression of corticosteroids. in the present study, we investigated the effects of pair-housing on wound healing and corticosteroid concentrations in three mouse species, the mon ...200515581739
different evolutionary trails in the related genomes cricetus cricetus and peromyscus eremicus (rodentia, cricetidae) uncovered by orthologous satellite dna repositioning.constitutive heterochromatin comprises a substantial fraction of the eukaryotic genomes and is mainly composed of tandemly arrayed satellite dnas (satdna). these repetitive sequences represent a very dynamic and fast evolving component of genomes. in the present work we report the isolation of cricetus cricetus (ccr, cricetidae, rodentia) centromeric repetitive sequences from chromosome 4 (ccr4/10sat), using the laser microdissection and laser pressure catapulting procedure, followed by dop-pcr ...200818602266
[occurrence of giardia species and genotypes in humans and animals in wielkopolska region, poland].giardia is the most common intestinal protozoan parasite found in humans and animals worldwide. although it has been known for three hundred years, the nomenclature, taxonomy, host specificity, and pathogenicity of giardia still arouse numerous controversies and ambiguities. giardia is classified into six species, that are characterised by various ranges of hosts. the most dubious species is g. intestinalis, which includes a dozen or so genotypes, and only two of them (genotype a and b) have wid ...200920209826
hidden heterochromatin: characterization in the rodentia species cricetus cricetus, peromyscus eremicus (cricetidae) and praomys tullbergi (muridae).the use of in situ restriction endonuclease (re) (which cleaves dna at specific sequences) digestion has proven to be a useful technique in improving the dissection of constitutive heterochromatin (ch), and in the understanding of the ch evolution in different genomes. in the present work we describe in detail the ch of the three rodentia species, cricetus cricetus, peromyscus eremicus (family cricetidae) and praomys tullbergi (family muridae) using a panel of seven res followed by c-banding. co ...200921637647
Prevalence and multilocus genotyping of Giardia from animals at the zoo of Poznan, Poland.In this study total of 266 fecal samples from 242 animals belonging to 113 species kept in the Poznan Zoological Garden were examined for Giardia. The cysts of Giardia were found only in five samples of feces collected from a giant toad (Bufo marinus), tamandua (Tamandua tetradactyla) and three individuals of cactus mouse (Peromyscus eremicus). Fragments of beta-giardin (bg), triose phosphate isomerase (tpi) and glutamate dehydrogenase (gdh) genes were successfully amplified only from the Giardi ...201122165739
polymorphism, genetic stability, and autosomal location of trimeric nucleoside phosphorylase in peromyscus eremicus cell lines.nucleoside phosphorylase (np: ec 2.4.2.1) has not been demonstrated to be an extensively polymorphic enzyme locus in mammals. we have studied np electrophoretically in five independently derived cell lines established from peromyscus eremicus as well as in various tissues of a sixth animal. four different np phenotypes involving three different alleles were resolved. the data suggest that (1) the enzyme is trimeric and its genetic locus is polymorphic in p. eremicus, (2) heterozygous enzyme phen ...1998109577
minimal metabolism, summit metabolism and plasma thyroxine in rodents from different environments.rodents were live-trapped in three environments (desert, intermediate and coastal) in southern california, usa, chosen because of the taxonomic overlap of species. upon capture, blood samples were taken and plasma thyroxine concentrations were measured. four species (dipodomys merriami, perognathus fallax, peromyscus eremicus and peromyscus californicus) were returned to the laboratory for measurement of minimal and summit rates of metabolism. heteromyid rodents had significantly lower plasma th ...20102863060
nematofauna of rodents of the families heteromyidae and cricetidae from the mexican plateau.as a part of an ongoing project to inventory the helminth parasites of rodents in mexico, 85 specimens of 2 families of rodents were collected from the mexican plateau: cricetidae ( neotoma sp., neotoma leucodon , onychomys arenicola , peromyscus sp., peromyscus eremicus , and reithrodontomys sp.) and heteromyidae ( chaetodipus sp., chaetodipus eremicus , chaetodipus hispidus , dipodomys merriami , dipodomys ordii , dipodomys ornatus, dipodomys spectabilis , liomys irroratus , perognathus sp., a ...201727788020
permeability of roads to movement of scrubland lizards and small mammals.a primary objective of road ecology is to understand and predict how roads affect connectivity of wildlife populations. road avoidance behavior can fragment populations, whereas lack of road avoidance can result in high mortality due to wildlife-vehicle collisions. many small animal species focus their activities to particular microhabitats within their larger habitat. we sought to assess how different types of roads affect the movement of small vertebrates and to explore whether responses to ro ...201323772966
assessment of mercury concentrations in small mammals collected near las vegas, nevada, usa.mercury concentrations in liver and hair tissue were determined for five species of small mammals captured near las vegas, nevada, usa. these data were then used to evaluate the suitability of using hair as a noninvasive technique for determining body burdens of mercury. a total of 104 small mammals were captured and analyzed; four main species were examined and included: dipodomys merriami, chaetodipus penicillatus, peromyscus eremicus, and neotoma lepida. mean mercury concentrations were highe ...200617091502
studies of mammalian chromosome replication. ii. evidence for the existence of defined chromosome replicating units.sister chromatids of metaphase chromosomes can be differentially stained if the cells have replicated their dna semiconservatively for two cell cycles in a medium containing 5-bromodeoxyuridine (brdu). when prematurely condensed chromosomes (pcc) are induced in cells during the second s phase after brdu is added to the medium, the replicated chromosome segments show sister chromatid differential (scd) staining. employing this pcc-scd system on synchronous and asynchronous chinese hamster ovary ( ...19817028418
a novel satellite dna sequence in the peromyscus genome (pmsat): evolution via copy number fluctuation.satellite dnas (satdna) are tandemly arrayed repeated sequences largely present in eukaryotic genomes, which play important roles in genome evolution and function, and therefore, their analysis is vital. here, we describe the isolation of a novel satellite dna family (pmsat) from the rodent peromyscus eremicus (cricetidae, rodentia), which is located in pericentromeric regions and exhibits a typical satellite dna genome organization. orthologous pmsat sequences were isolated and characterized fr ...201526103000
a high-resolution comparative chromosome map of cricetus cricetus and peromyscus eremicus reveals the involvement of constitutive heterochromatin in breakpoint regions.compared to humans and other mammals, rodent genomes, specifically muroidea species, underwent intense chromosome reshuffling in which many complex structural rearrangements occurred. this fact makes them preferential animal models for studying the process of karyotype evolution. here, we present the first combined chromosome comparative maps between 2 cricetidae species, cricetus cricetus and peromyscus eremicus, and the index species mus musculus and rattus norvegicus. comparative chromosome p ...201525999143
line-1 retrotransposons: from 'parasite' sequences to functional elements.long interspersed nuclear elements-1 (line-1) are the most abundant and active retrotransposons in the mammalian genomes. traditionally, the occurrence of line-1 sequences in the genome of mammals has been explained by the selfish dna hypothesis. nevertheless, recently, it has also been argued that these sequences could play important roles in these genomes, as in the regulation of gene expression, genome modelling and x-chromosome inactivation. the non-random chromosomal distribution is a strik ...201525106509
cacti supply limited nutrients to a desert rodent community.in the sonoran desert, cacti represent a potentially important source of nutrients and water for consumers. columnar cacti, in particular, produce a large pulse of flowers and succulent fruit during hot summer months. the importance of cactus stems, flowers and fruit to the small mammal community has not been quantified. we exploited natural variation in the carbon isotope (δ(13)c) values of cacti (cam) versus c3 plants to quantify the relative use of these resources by a diverse desert small ma ...201525842296
characterization of a male reproductive transcriptome for peromyscus eremicus (cactus mouse).rodents of the genus peromyscus have become increasingly utilized models for investigations into adaptive biology. this genus is particularly powerful for research linking genetics with adaptive physiology or behaviors, and recent research has capitalized on the unique opportunities afforded by the ecological diversity of these rodents. well characterized genomic and transcriptomic data is intrinsic to explorations of the genetic architecture responsible for ecological adaptations. therefore, th ...201627812417
characterizing the reproductive transcriptomic correlates of acute dehydration in males in the desert-adapted rodent, peromyscus eremicus.the understanding of genomic and physiological mechanisms related to how organisms living in extreme environments survive and reproduce is an outstanding question facing evolutionary and organismal biologists. one interesting example of adaptation is related to the survival of mammals in deserts, where extreme water limitation is common. research on desert rodent adaptations has focused predominantly on adaptations related to surviving dehydration, while potential reproductive physiology adaptat ...201728645248
severe acute dehydration in a desert rodent elicits a transcriptional response that effectively prevents kidney injury.animals living in desert environments are forced to survive despite severe heat, intense solar radiation, and both acute and chronic dehydration. these animals have evolved phenotypes that effectively address these environmental stressors. to begin to understand the ways in which the desert-adapted rodent peromyscus eremicus survives, reproductively mature adults were subjected to 72 h of water deprivation, during which they lost, on average, 23% of their body weight. the animals reacted via a s ...201728381460
physiological and biochemical changes associated with acute experimental dehydration in the desert adapted mouse, peromyscus eremicus.characterizing traits critical for adaptation to a given environment is an important first step in understanding how phenotypes evolve. how animals adapt to the extreme heat and aridity commonplace to deserts is an exceptionally interesting example of these processes, and has been the focus of study for decades. in contrast to those studies, where experiments are conducted on either wild animals or captive animals held in non-desert conditions, the study described here leverages a unique environ ...201728330954
characterization of the transcriptome, nucleotide sequence polymorphism, and natural selection in the desert adapted mouse peromyscus eremicus.as a direct result of intense heat and aridity, deserts are thought to be among the most harsh of environments, particularly for their mammalian inhabitants. given that osmoregulation can be challenging for these animals, with failure resulting in death, strong selection should be observed on genes related to the maintenance of water and solute balance. one such animal, peromyscus eremicus, is native to the desert regions of the southwest united states and may live its entire life without oral f ...201425374784
phylogeography and systematics of the peromyscus eremicus species group and the historical biogeography of north american warm regional deserts.phylogeographic relationships among 26 populations from throughout the geographic range of the peromyscus eremicus species group are described based on sequence data for a 699-bp fragment of the mitochondrial dna coiii gene. distance, maximum-likelihood, and maximum-parsimony analyses of phylogenetic trees generated under four separate character-weighting strategies and representing five alternative biogeographic hypotheses revealed the existence of a cryptic species (peromyscus fraterculus, pre ...200011083930
among-site rate variation and phylogenetic analysis of 12s rrna in sigmodontine rodents.we analyze sequences from two mitochondrial genes, cytochrome b (cyt b) and 12s rrna (12s), for a group of sigmodontine rodents among which phylogenetic relationships are well understood based on concordance of morphological, chromosomal, allozyme, and other dna data sets. because these two genes are physically linked on the nonrecombining mitochondrial genome, they necessarily share the same history. phylogenetic analysis of the cyt b gene recovers the well-corroborated relationships, generally ...19958524051
reproductive inhibition in female cactus mice (peromyscus eremicus). 1978712256
studies in heterochromatin dna: characterization of transcripts synthesized in situ from c-banded preparations.the transcript of in situ rna synthesis using peromyscus eremicus cells, which had been treated with hcl followed by naoh, proved to be rich in heterochromatin specifying sequences. such transcript, referred to as heterochromatin rna, can be characterized biochemically to probe the organization of heterochromatin dna. it was found that the heterochromatin rna of p. eremicus contained non-repetitive sequences covalently linked to repetitive sequences.1978648279
determinants of ejaculatory failure in the copulatory behavior of cactus mice (peromyscus eremicus). 1976964442
copulatory behavior of wild-trapped and laboratory-reared cactus mice (peromyscus eremicus) from two natural populations. 19744412637
laboratory life histories of peromyscus eremicus and peromyscus interparietalis. 19685670808
notes on reproduction of peromyscus eremicus in a laboratory colony. 194720245732
molecular detection of leptospira interrogans and borrelia burgdorferi in wild rodents from mexico.leptospirosis and lyme borreliosis are zoonotic emerging diseases of global importance and wide distribution. the aim of this study was to detect by molecular testing to leptospira interrogans and borrelia burgdorferi sensu lato in wild rodents from nuevo leon, quintana roo, and campeche, mexico. this study is the first in report to chaetodipus nelsoni, dipodomys merriami, and peromyscus eremicus infected with l. interrogans in mexico. besides, chaetodipus hispidus, heteromys gaumeri, heteromys ...202032639187
novel focus of sin nombre virus in peromyscus eremicus mice, death valley national park, california, usa.the deer mouse (peromyscus maniculatus) is the primary reservoir for sin nombre virus (snv) in the western united states. rodent surveillance for hantavirus in death valley national park, california, usa, revealed cactus mice (p. eremicus) as a possible focal reservoir for snv in this location. we identified snv antibodies in 40% of cactus mice sampled.201829774841
comparative and population genomics approaches reveal the basis of adaptation to deserts in a small rodent.organisms that live in deserts offer the opportunity to investigate how species adapt to environmental conditions that are lethal to most plants and animals. in the hot deserts of north america, high temperatures and lack of water are conspicuous challenges for organisms living there. the cactus mouse (peromyscus eremicus) displays several adaptations to these conditions, including low metabolic rate, heat tolerance, and the ability to maintain homeostasis under extreme dehydration. to investiga ...202032130752
a tale of two valleys: disparity in sin nombre virus antibody reactivity between neighboring mojave desert communities.hantaviruses are a group of globally distributed rodent-associated viruses, some of which are responsible for human morbidity and mortality. sin nombre orthohantavirus, a particularly virulent species of hantavirus associated with peromyscus spp. mice, is actively monitored by the department of public health in california (cdph). recently, cdph documented high (40%) seroprevalence in a potentially novel reservoir species, the cactus mouse (peromyscus eremicus) in death valley national park.201930932773
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