| dna relatedness among strains of the sweet potato pathogen streptomyces ipomoea (person and martin 1940) waksman and henrici 1948. | dna relatedness among 28 putative strains of streptomyces ipomoea from geographically diverse locations and the type strain, nrrl b-12321, was determined spectrophotometrically. the data confirm that these 28 strains are not closely related genetically to the plant-pathogenic species streptomyces scabies (39% dna relatedness) or streptomyces acidiscabies (17% dna relatedness) or any other major blue-spored streptomyces species (less than 30% dna relatedness). of the 29 strains examined, 4 could ... | 1992 | 1610178 |
| phylogenetic analysis of streptomyces spp. causing potato scab based on 16s rrna sequences. | the complete 16s rrna sequences of 12 strains of streptomyces spp., including potato scab pathogens, were determined. among the strains of streptomyces scabies that were isolated from diverse geographical areas and differed in melanoid pigment production, either no difference or one difference in sequence was observed. the sequence of s. scabies was most similar to the sequences of streptomyces diastatochromogenes, streptomyces bottropensis, and streptomyces neyagawaensis, which belong to the di ... | 1996 | 8934906 |
| cloning and expression of a gene from streptomyces scabies encoding a putative pathogenicity factor. | we cloned a 9.4-kb dna fragment from streptomyces scabies atcc 41973 that allows the nonpathogen streptomyces lividans 66 tk24 to necrotize and colonize potato tuber slices and produce scab-like symptoms on potato minitubers. deletion analysis demonstrated that activity was conferred by a 1.6-kb dna region. sequence analysis of a 2.4-kb dna fragment spanning the dna region necessary for activity revealed three open reading frames (orfs). the deduced amino acid sequence of orf1, designated orftnp ... | 1997 | 9401037 |
| dna relatedness among strains of streptomyces pathogenic to potato in france: description of three new species, s. europaeiscabiei sp. nov. and s. stelliscabiei sp. nov. associated with common scab, and s. reticuliscabiei sp. nov. associated with netted scab. | the genomic relatedness was evaluated by dna-dna hybridization for 23 strains (21 were pathogenic and two were saprophytic strains) isolated from lesions of common and netted scab in france and 19 strains from other countries, including type strains of streptomyces species. three genomospecies were defined within the conventional species of streptomyces scabies, and these genomospecies were different from other pathogenic described species (streptomyces acidiscabies, streptomyces caviscabies) ba ... | 2000 | 10826791 |
| the txtab genes of the plant pathogen streptomyces acidiscabies encode a peptide synthetase required for phytotoxin thaxtomin a production and pathogenicity. | four streptomyces species have been described as the causal agents of scab disease, which affects economically important root and tuber crops worldwide. these species produce a family of cyclic dipeptides, the thaxtomins, which alone mimic disease symptomatology. structural considerations suggest that thaxtomins are synthesized non-ribosomally. degenerate oligonucleotide primers were used to amplify conserved portions of the acyladenylation module of peptide synthetase genes from genomic dna of ... | 2000 | 11115114 |
| isolation and identification of pathogenic strains of streptomyces acidiscabies from netted scab lesions of potato tubers in hokkaido (japan). | a relatively homogeneous group of streptomycete isolates was obtained from netted scab lesions of potato tubers collected from a potato field in hokkaido, japan. based on 55 phenotypic data of 72 streptomyces strains selected from these isolated together with spectral data on their soluble pigments and with data of a pcr analysis, using species specific primers, these netted scab causing pathogenic organisms were identified as s. acidiscabies. s. acidiscabies had previously been isolated from de ... | 2001 | 11791352 |
| involvement of a cytochrome p450 monooxygenase in thaxtomin a biosynthesis by streptomyces acidiscabies. | the biosynthesis of the thaxtomin cyclic dipeptide phytotoxins proceeds nonribosomally via the thiotemplate mechanism. acyladenylation, thioesterification, n-methylation, and cyclization of two amino acid substrates are catalyzed by the txtab-encoded thaxtomin synthetase. nucleotide sequence analysis of the region 3' of txtab in streptomyces acidiscabies 84.104 identified an open reading frame (orf) encoding a homolog of the p450 monooxygenase gene family. it was proposed that thaxtomin a phenyl ... | 2002 | 11889110 |
| phylogenetic analysis of streptomyces spp. isolated from potato scab lesions in korea on the basis of 16s rrna gene and 16s-23s rdna internally transcribed spacer sequences. | the 16s rrna gene sequences for 34 strains, including 11 isolates, were determined to classify scab-causing streptomyces spp. and relatives isolated from potato scab lesions collected in jeju, korea. the 16s-23s rdna internally transcribed spacer (its) sequences were determined to investigate whether the 16s-23s its region is useful for analysing intra- and interspecific relationships in these bacteria. on the basis of phylogenetic analysis of 16s rrna gene sequences, most of the isolates were c ... | 2004 | 14742481 |
| a large, mobile pathogenicity island confers plant pathogenicity on streptomyces species. | potato scab is a globally important disease caused by polyphyletic plant pathogenic streptomyces species. streptomyces acidiscabies, streptomyces scabies and streptomyces turgidiscabies possess a conserved biosynthetic pathway for the nitrated dipeptide phytotoxin thaxtomin. these pathogens also possess the nec1 gene which encodes a necrogenic protein that is an independent virulence factor. in this article we describe a large (325-660 kb) pathogenicity island (pai) conserved among these three p ... | 2005 | 15686551 |
| superoxide dismutases of heavy metal resistant streptomycetes. | heavy metal tolerant and resistant strains of streptomycetes isolated from a former uranium mining site were screened for their superoxide dismutase expression. from the strains tolerating high concentrations of different heavy metals, one was selected for its tolerance of concentrations of heavy metals (ni, cu, cd, cr, mn, zn, fe). this strain, streptomyces acidiscabies e13, was chosen for the purpose of superoxide dismutase analysis. gel electrophoresis and activity staining revealed only one ... | 2007 | 17304620 |
| effect of carbohydrates on the production of thaxtomin a by streptomyces acidiscabies. | several streptomyces species cause plant diseases, including s. scabies, s. acidiscabies and s. turgidiscabies, which produce common scab of potato and similar diseases of root crops. these species produce thaxtomins, dipeptide phytotoxins that are responsible for disease symptoms. thaxtomins are produced in vivo on diseased potato tissue and in vitro in oat-based culture media, but the regulation of thaxtomin biosynthesis is not understood. s. acidiscabies was grown in a variety of media to ass ... | 2007 | 17340119 |
| hydroxamate siderophores produced by streptomyces acidiscabies e13 bind nickel and promote growth in cowpea (vigna unguiculata l.) under nickel stress. | the siderophore-producing ability of nickel-resistant streptomyces acidiscabies e13 and the role of the elicited siderophores in promoting plant growth under iron and nickel stress are described. siderophore assays indicated that s. acidiscabies e13 can produce siderophores. electrospray ionization mass spectrometry (esi-ms) revealed that the bacterium simultaneously produces 3 different hydroxamate siderophores. esi-ms showed that in addition to iron, all 3 siderophores can bind nickel. in vitr ... | 2008 | 18388987 |
| "ni-struvite" - a new biomineral formed by a nickel resistant streptomyces acidiscabies. | biomineralization dependent on bacterial activity has been described for struvite which is formed in soils, guano, putrescent matter and sediments. a new biomineral containing nickel instead of magnesium, ni(nh4)(po4) . 6h2o, has been identified. it was formed by nickel resistant streptomyces acidiscabies e13, and putatively named nickel struvite. the mineral formation is dependent on biological activity since non-viable bacterial cells are not capable to induce formation of ni-struvite under id ... | 2008 | 18410951 |
| genetic diversity of streptomyces spp. causing common scab of potato in eastern canada. | common scab is an important disease of potato caused by streptomyces scabies and other closely related species. in this study, the genetic diversity of streptomyces spp. causing common scab of potato in eastern canada was for the first time investigated. forty-one streptomyces spp. isolates were retrieved from necrotic lesions of potato tubers harvested from different regions of the canadian provinces new-brunswick, nova scotia and prince-edward-island. most isolates were closely related to know ... | 2008 | 18947953 |
| characterization of gamma-butyrolactone autoregulatory signaling gene homologs in the angucyclinone polyketide ws5995b producer streptomyces acidiscabies. | organisms belonging to the genus streptomyces produce numerous important secondary metabolites and undergo a sophisticated morphological differentiation program. in many instances these processes are under the control of gamma-butyrolactone (gbl) autoregulatory systems. streptomyces acidiscabies strain 84.104 produces the secondary metabolite aromatic angucyclinone polyketide ws5995b. in order to explore the role of gbl regulatory circuitry in ws5995b production and morphogenesis in s. acidiscab ... | 2009 | 19465647 |
| isolation and characterization of antagonistic fungi against potato scab pathogens from potato field soils. | potato scab is a serious plant disease caused by several streptomyces sp., and effective control methods remain unavailable. although antagonistic bacteria and phages against potato scab pathogens have been reported, to the best of our knowledge, there is no information about fungi that are antagonistic to the pathogens. the aim of this study was to isolate fungal antagonists, characterize their phylogenetic positions, determine their antagonistic activities against potato scab pathogens, and hi ... | 2010 | 20653777 |
| multilocus sequence analysis of phytopathogenic streptomyces species. | the identification and classification of species within the genus streptomyces is difficult because there are presently 576 species with validly published names and this number increases every year. the value of the application of multilocus sequence analysis scheme to the systematics of streptomyces species has been well demonstrated in several recently published papers. in this study the sequence fragments of 4 housekeeping genes, atpd, reca, rpob, and trpb, were determined for the type strain ... | 2010 | 21112986 |
| pioneering particle-based strategy for isolating viable bacteria from multipart soil samples compatible with raman spectroscopy. | the study of edaphic bacteria is of great interest, particularly for evaluating soil remediation and recultivation methods. therefore, a fast and simple strategy to isolate various bacteria from complex soil samples using poly(ethyleneimine) (pei)-modified polyethylene particles is introduced. the research focuses on the binding behavior under different conditions, such as the composition, ph value, and ionic strength, of the binding buffer, and is supported by the characterization of the surfac ... | 2017 | 28364142 |
| acidiscalide, a new glycosylated macrolide from the marine actinomycete streptomyces acidiscabies. | a new macrolide named acidiscalide (2), and a known protein synthesis inhibitor, cycloheximide (1), were isolated from the culture broth of a strain of actinomycete, streptomyces acidiscabies, coded scta0002 collected from the coast of okinawa. the structure of acidiscalide (2) was elucidated to be a glycosylated macrolide having a 24-membered ring on the basis of spectroscopic analysis. although the stereochemistry could not be determined due to decomposition in the course of the study, it was ... | 2016 | 27032206 |
| draft genome sequences of streptomyces scabiei s58, streptomyces turgidiscabies t45, and streptomyces acidiscabies a10, the pathogens of potato common scab, isolated in japan. | the draft genome sequences of the three pathogens of potato common scab, streptomyces scabiei s58, streptomyces turgidiscabies t45, and streptomyces acidiscabies a10, isolated in japan, are presented here. the genome size of each strain is >10 mb, and the three pathogenic strains share genes located in a pathogenicity island previously described in other pathogenic streptomyces species. | 2016 | 26941144 |
| identification of genetic and environmental factors stimulating excision from streptomyces scabiei chromosome of the toxicogenic region responsible for pathogenicity. | the genes conferring pathogenicity in streptomyces turgidiscabies, a pathogen causing common scab of potato, are grouped together on a pathogenicity island (pai), which has been found to be mobile and appears to transfer and disseminate like an integrative and conjugative element (ice). however, in streptomyces scabiei, another common scab-inducing species, the pathogenicity genes are clustered in two regions: the toxicogenic region (tr) and the colonization region. the s. scabiei 87.22 genome w ... | 2016 | 26177341 |
| phytoremediation using microbially mediated metal accumulation in sorghum bicolor. | reclaiming land that has been anthropogenically contaminated with multiple heavy metal elements, e.g., during mining operations, is a growing challenge worldwide. the use of phytoremediation has been discussed with varying success. here, we show that a careful examination of options of microbial determination of plant performance is a key element in providing a multielement remediation option for such landscapes. we used both (a) mycorrhiza with rhizophagus irregularis and (b) bacterial amendmen ... | 2015 | 25874434 |
| identification of a novel unpaired histidine sensor kinase affecting secondary metabolism and morphological differentiation in streptomyces acidiscabies atcc 49003. | two-component systems (tcss) are an important signaling transduction pathway that adapt to changing environments. commonly, a tcs comprises a sensor kinase that is usually an integral membrane histidine sensor kinase and a response regulator that mediates the cellular responses. presently, however, we cloned a novel sensor kinase gene (tcsk) that is not adjacent to its cognate response regulator from streptomyces acidiscabies that produces two secondary metabolites, thaxtomin a and ws5995b, and ... | 2015 | 25821125 |
| the cellobiose sensor cebr is the gatekeeper of streptomyces scabies pathogenicity. | a relatively small number of species in the large genus streptomyces are pathogenic; the best characterized of these is streptomyces scabies. the pathogenicity of s. scabies strains is dependent on the production of the nitrated diketopiperazine thaxtomin a, which is a potent plant cellulose synthesis inhibitor. much is known about the genetic loci associated with plant virulence; however, the molecular mechanisms by which s. scabies triggers expression of thaxtomin biosynthetic genes, beyond th ... | 2015 | 25714708 |
| characterization of the integration and modular excision of the integrative conjugative element paist in streptomyces turgidiscabies car8. | paist is a large genomic island located in the chromosome of the plant pathogen streptomyces turgidiscabies car8. the island carries clustered virulence genes, transfers to other streptomyces species, and integrates by site-specific recombination at the 8 bp palindrome ttcatgaa. the palindrome is located at the 3' end of the bacitracin resistance gene (baca). we demonstrate that paist is able to excise in modules by recombination of one internal and two flanking palindromic direct repeats. the g ... | 2014 | 24927117 |
| a species-specific method for detecting pathogenic streptomyces species from soil and potato tubers in argentina. | potato common scab is caused by several soil-inhabiting pathogenic streptomyces species. in the present study, a species-specific pcr method was used to detect streptomyces species in potato tuber lesions and soils. total genomic dna from soil samples from six locations and tuber samples from four potato cultivars (spunta, shepody, innovator and russet burbank) were assessed. streptomyces scabies, streptomyces acidiscabies, and streptomyces turgidiscabies were detected in soybean, tobacco and po ... | 2014 | 24401784 |
| draft genome sequence of streptomyces acidiscabies 84-104, an emergent plant pathogen. | a draft genome sequence of the plant pathogen streptomyces acidiscabies 84-104, an emergent plant pathogen, is presented here. the genome is among the largest of streptomycetes, at more than 11 mb, and encodes a 100-kb pathogenicity island (pai) shared with other plant-pathogenic streptomycetes. the presence of this conserved pai, and the remnants of a conserved integrase/recombinase at its 3' end, supports the hypothesis that s. acidiscabies emerged as a plant pathogen as a result of this acqui ... | 2012 | 22408247 |