| studies on the diagnosis and treatment of human filariasis in rhodesia. | experiences in rhodesia with various recovery techniques available for the laboratory diagnosis of infections with dipetalonema perstans and wuchereria bancrofti are discussed. a diagnostic laboratory regimen for routine filarial investigations is suggested. included are preliminary observations on the use of mebendazole (vermox) for the treatment of d. perstans infections. | 1976 | 60784 |
| [immunological diagnosis of filariosis in persons returning from tropical countries and in european and indigenous inhabitants of endemic areas (author's transl)]. | serum samples from persons who lived in areas where onchocerciasis occurred or who had filariasis were examined with the complement fixation test and the indirect hemagglutination test for the presence of antibodies against crude extracts from dirofilaria immitis, onchocerca volvulus, dipetalonema viteae, and ascaris suum. the results could be interpreted as follows: 1. the indirect hemagglutination test was more sensitive than the complement fixation test for the demonstration of antibodies in ... | 1977 | 271633 |
| tanzania filariasis project: a provocative day test with diethylcarbamazine for the detection of microfilariae of nocturnally periodic wuchereria bancrofti in the blood. | in coastal tanzania, an area where the microfilariae (mf) of wuchereria bancrofti exhibit nocturnal periodicity, the administration of 2 mg diethylcarbamazine (dec) per kg body weight in the daytime provoked mf to enter the peripheral blood. in persons on normal daily activities the daytime dec provocative method proved to be as sensitive in detecting microfilaraemia as was the examination of night blood. its use in routine surveys is therefore justified. although mf densities by day and night w ... | 1979 | 396052 |
| some transfusion-induced parasitic infections in zambia. | the risk of acquiring a transfusion-induced infection in zambia was studied for the first time. blood slide examination of donors, despite the insensitivity of the method, established malaria as the most serious hazard. the species involved was plasmodium falciparum, the cause of cerebral malaria, and which could be rapidly fatal in a non-immune host visiting an endemic area. microfilariae of dipetalonema perstans and wuchereria bancrofti were also found in donor populations. while no disease ma ... | 1979 | 399289 |
| mebendazole. | the broad-spectrum of activity and safety of mebendazole remain, after 5 years of clinical experience, unique features of this anthelmintic. through microtubular destruction, mebendazole kills helminths by inhibiting glucose uptake into susceptible parasites. the drug's poor absorption does not appear to affect clinical efficacy except, perhaps, in the treatment of systemic helminth infections. mebendazole is generally considered the drug of choice for trichuriasis and has therapeutic advantages ... | 1979 | 484964 |
| localization of acid phosphatase in microfilariae of loa loa and dipetalonema perstans from cameroun. | the histochemical pattern of acid phosphatase activity in the microfilaria of l. loa showed a diffuse red staining all over the body, with conspicuous staining of the excretory and anal vesicles, innenkörper, amphids and phasmids. in d. perstans, the enzymatic activity was localized chiefly in four regions corresponding to the excretory and anal vesicles, amphids and phasmids of the microfilariae. a considerable loss of enzyme activity was observed in microfilariae that had been stored in a refr ... | 1977 | 564090 |
| a preliminary study on the treatment of filariasis due to dipetalonema perstans. | | 1979 | 571764 |
| epidemiology of poly-parasitism. ii. types of combinations, relative frequency and associations of multiple infections. | frequency distributions, including all of the observed types of combinations of multiple infections with different helminths and protozoa are presented for sample villages of the african savannah and of the trans-andean part of peru. measurements of correlation between these infections based on properties of the multivariate, multinomial distribution are calculated to show age and sex patterns of association in different population samples. a correlation matrix for combined infections with dipet ... | 1978 | 675834 |
| dipetalonema perstans and mansonella ozzardi in indians of southern venezuela. | microfilariae were observed in 25 of 187 blood leucocyte-culture preparations made for chromosome studies on 28 piaroa and 159 yanomama indians living near the brazilian border of amazonas, venezuela. among the yanomama, 17 (10.7%) were infected, all with mansonella ozzardi. among the piaroa, 8 (28.6%) were infected--3 with m. ozzardi, 4 with dipetalonema perstans, and 1 with both species. | 1976 | 769579 |
| man-biting species of chrysops meigen, culicoides latreille and simulium latreille in ethiopia, with discussion of their vector potentialities. | human filariasis and its vectors are little known in ethiopia. wuchereia bancrofti is confined to the western lowlands and cannot be the aetiological agent of elephantiasis in the highlands. onchocerca volvulus is widespread in the south-western highlands. loa loa and dipetalonema perstans are reported here for the first time and appear to be uncommon. studies were conducted in 1973, mostly near jimma in kaffa province, on potential vectors of all but the first of these parasites. among simuliid ... | 1977 | 877992 |
| wuchereria bancrofti: the staining of the microfilarial sheath in giemsa and haematoxylin for diagnosis. | indigenous cases of bancroftian filariasis have recently been identified in the country for the first time. the diagnosis of such infections depends on demonstrating the characteristic sheathed microfilariae of wuchereria bancrofti in the blood. giemsa, a stain recommended by many authors, was found to be unsuitable as the microfilarial sheath, an important distinguishing feature, does not take up the stain. in contrast, the sheath stains clearly in haematoxylin. the differences in the staining ... | 1977 | 919785 |
| effect of mebendazole and levamisole on onchocerca volvulus and dipetalonema perstans. | | 1975 | 1237183 |
| specific and sensitive igg4 immunodiagnosis of onchocerciasis with a recombinant 33 kd onchocerca volvulus protein (ov33). | the full length cdna of the immunodominant ov33 protein of onchocerca volvulus was expressed in e. coli using various vector constructs. expression was best with the vectors pgex2t and pcg808fx, yielding fusion protein ov33-gst and ov33-mbp, respectively. purified fusion protein ov33-gst and o. volvulus antigen extracts (ovag) were used to compare antibody responses (igm and igg-subclasses) of patients infected with o. volvulus, brugia malayi, wuchereria bancrofti, mansonella perstans/loa loa an ... | 1992 | 1281926 |
| identification and characterization of a parasite antigen in the circulating immune complexes of onchocerca volvulus infected patients. | a peg-elisa was used to demonstrate parasite specific immune complexes in a significant proportion (25/26) of onchocerca volvulus infection sera from sierra leone. the parasite antigen was detected using a peroxidase-conjugated rabbit serum raised to the bovine parasite o. gibsoni. controls including european control serum, endemic control serum and rh+ sera gave consistently low readings. characterization of the parasite component in the immune complexes by western blotting demonstrated a heat ... | 1992 | 1284095 |
| filariasis in gongola state nigeria. i: clinical and parasitological studies in mutum-biyu district. | a total of 2,552 persons living in 9 villages along the benue river valley, mutum biyu district of gongola state, nigeria were examined between october and december 1989 for filariasis. it is the first time a filariasis survey will be carried out in this state. 276 (10.8%) had wuchereria bancrofti, 50 (2.0%) had loa loa, 281 (11.0%) were positive for mansonella perstans while 12 (0.5%) were positive for onchocerca volvulus. villages located near the benue river had higher prevalence rates than t ... | 1992 | 1416217 |
| observations on mansonellosis among the ibos of abia and imo states, nigeria. | between november, 1988 and april, 1991, parasitological and symptomatological methods of diagnosis were used to survey the prevalence of mansonellosis among the ibo population in abia and imo states of nigeria. 1,197 or 28.6% of the 4,183 persons examined were positive for microfilariae of mansonella perstans. the prevalence of mansonellosis was significantly higher (p < 0.05) among rural dwellers (34.6%) than among urban dwellers (22.5%), in males (30.8%) than in females (26.3%), in farmers (59 ... | 1992 | 1456469 |
| mansonella perstans filariasis: failure of albendazole treatment. | infections with mansonella perstans are common in certain parts of africa and south america. there is no standard treatment at present. we evaluated the effect of albendazole on microfilaremia in ten consecutive patients. no decrease in microfilarial counts could be demonstrated after a median follow-up period of 45 days. albendazole was not shown to be useful for treatment of mansonella perstans filariasis. | 1992 | 1476470 |
| human ecology and parasitic infections. 1. the effect of occupation on the prevalence of parasitic infections in calabar, nigeria. | one thousand six hundred people belonging to three different occupational groups were randomly selected. blood, urine and stool specimens were collected from them and processed for the detection of any parasitic infections. the results show 28.5% infection rate with one or more species of intestinal parasites; 50.7% in the school children, 17.3% among the farmers, and 12% among the soldiers. results of blood examination showed 5.3%, 8.0% and 2.0% infection rates for p. falciparum in the school p ... | 1992 | 1512451 |
| population dynamics of loa loa and mansonella perstans infections in individuals living in an endemic area of the congo. | a follow-up of loa loa and mansonella perstans microfilaremia was carried out in an adult population living in a highly endemic area of the congo. infection rates and parasite loads were found to be stable in the general population, both in the short-term (two months) and long-term (3-4 years) followup. the microfilarial status of most of the subjects examined did not change between tests. at the individual level, the microfilarial densities of l. loa and m. perstans also remained remarkably con ... | 1992 | 1621891 |
| filariasis in gongola state nigeria. i: clinical and parasitological studies in mutum-biyu district. | a total of 2552 persons living in 9 villages along the benue river valley, mutum-biyu district of gongola state, nigeria were examined between october and december 1989 for filariasis. it is the first time a filariasis survey will be carried out in this state. 276 (10.8%) had wuchereria bancrofti, 50 (2.0%) had loa loa, 281 (11.0%) were positive for mansonella perstans while 12 (0.5%) were positive for onchocerca volvulus. villages located near the benue river had higher prevalence rates than th ... | 1991 | 1804869 |
| molecular cloning and characterization of recombinant parasite antigens for immunodiagnosis of onchocerciasis. | immunological cross-reactivity among nematodes has hampered the development of specific serodiagnostic assays for onchocerciasis. in the present study, an onchocerca volvulus adult worm complementary dna expression library was differentially screened with human sera from patients infected with o. volvulus and with an omnibus anti-nematode serum pool comprised of sera from patients infected with brugia malayi, loa loa, wuchereria bancrofti, mansonella perstans, strongyloides stercoralis, ancylost ... | 1991 | 1840605 |
| [allergic granulomatous nodule of the conjunctiva disclosing onchocerciasis]. | a new case of conjunctival allergic nodule is reported in a guinean man. this lesion was first described by ashton and cook in 1979. histologically, this nodule consists of amorphous eosinophilic material surrounded by epithelioid and giant cells arranged in a pallisade; some eosinophils are often found in the inflammatory reaction. this lesion usually resolves spontaneously. in documented cases, nematodes and particularly filaria such as mansonella perstans are usually isolated. our observation ... | 1991 | 1880338 |
| experimental infection of humans with filariae. | this report summarizes the findings of the 17 published studies involving humans who have been experimentally infected with filarial parasites. over the past 60 years, 45 individuals have been deliberately infected with wuchereria bancrofti, brugia malayi, brugia pahangi, loa loa, mansonella perstans, mansonella ozzardi, and/or onchocerca volvulus. the findings from these experimental infections of humans have helped define microfilarial survival and periodicity within human hosts, the prepatent ... | 1991 | 1962076 |
| the occurrence of loiasis, mansonellosis and wuchereriasis in the jarawa river valley, central nigeria. | in a study to assess the status of filariasis in the jarawa river valley of the savanna area of nigeria, 940 self-selected residents from 10 villages were examined between march 1984 and april 1987. overall results showed 105 (11.2%) had microfilaraemia and/or clinical signs of filariasis. sixty-four (6.8%) and 20 (2.1%) persons, respectively, were infected with mansonella perstans and loa loa microfilariae (mff). four of the 28 villagers examined had wuchereria bancrofti mff in their night bloo ... | 1990 | 1980569 |
| development of mansonella perstans in the surrogate vector aedes aegypti. | the biology of the human filaria mansonella perstans has been poorly studied due in part to the lack of experimental animal models in which its life-cycle could be reproduced. in nature culicodes spp. (and probably simuliids) are the vectors involved in disease transmission. in the present work aedes aegypti black-eyed strain was experimentally infected with m. perstans microfilariae by intrathoracic inoculation of 8 to 15 parasites contained in 0.4-0.6 microliters of rpmi 1640. concentration of ... | 1990 | 2075386 |
| [serum filariasis due to loa loa and mansonella perstans in the continental area of equatorial guinea. preliminary results]. | for the first time, a parasitologic survey has been carried out in the niefang district (continental area of equatorial guinea) within a sample of 829 people aged of more than 10 years. parasitologic incidence was 27.1 p.c. for loa-loa and 66.3 p.c. for mansonella perstans. significant differences were noted in the prevalence and in the mean density of microfilariae of both species according to the altitude of the villages surveyed. | 1990 | 2077317 |
| [a group of african students parasitized with mansonella perstans in cuba (a non-endemic area) from 1979 to 1982]. | sixty cases of manzonella perstans were studied during a three year period by clinical and blood parasitologic studies. it was observed that 31.2 was negative and that mean microfilaremia decreased from 49.3, at the beginning of the study, to 32.9 at the end of it, in those cases yet positive. according to the persistent microfilaremia, which means a relative longevity of adult parasites, the surveillance of individuals suffering it is recommended at their arrival to a non-endemic area where the ... | 1990 | 2089504 |
| epidemiology of mansonella perstans filariasis in the forest region of south congo. | a study of mansonella perstans filariasis conducted in the chaillu mountains, southern congo, showed that 108 of 134 pygmies (80.6%) and 79 of 302 bantus (26.2%) presented with microfilaraemia. the mean microfilarial densities were also significantly higher in the pygmies (1213 ml-1 of blood) than in the bantus (136 ml-1). ninety eight per cent of the culicoides taken which had bitten man in the daytime were c. grahamii, and 0.8% of these were infected with filarial larvae. two other species of ... | 1990 | 2222027 |
| [larval stages of the filaria mansonella (dipetalonema) perstans in a student from africa]. | microfilariae were found in blood samples of a 32 year-old-student from guinea-bissau (africa). based on parasite morphology as well as some other features the microfilariae were identified as mansonella perstans. the course of infection was asymptomatic (except for slight urticarial skin lesions). the microfilariae disappeared from peripheral blood after mebendazole treatment (200 mg daily for 1 month). | 1990 | 2224971 |
| [indirect immunofluorescence in filariasis. iii. comparison of microfilaremia and treatment. cuba, 1983]. | twenty six patients coming from endemic areas, with diagnosis of filariasis caused by wuchereria bancrofti, loa loa and manzonella (dipetalonema perstans) and 29 patients suspicious of suffering filariasis were studied by means of knott, membrane filter and indirect immunofluorescence techniques. results of microfilaremia were compared with antibody titers and it was verified that there was not relationship between these parameters. the suspicious patients presented higher antibody levels than t ... | 1990 | 2259783 |
| comparison of thick blood smear and saponin haemolysis for the detection of loa loa and mansonella perstans infections. | in an endemic area, loa loa and mansonella perstans microfilariae were detected by the examination of 5 ml blood in respectively 7.4 and 26.2% of subjects who would have been erroneously considered amicrofilaraemic by the conventional method of two thick blood smears (40 microliters blood). correction factors to be applied to the results obtained with thick blood films in order to approach the true parasitological prevalences were 1.5 for l. loa and 1.6 for m. perstans. in addition, the analysis ... | 1990 | 2270002 |
| clinical manifestations of loiasis in an endemic area in the congo. | the functional symptomatology of loiasis was studied in 101 congolese subjects living in a village in a highly endemic area. 27.7% of them were carriers of loa loa microfilariae. 5.9% were infected with mansonella perstans. no subjects were found to have dermal microfilariae. in anamnesis, 51.5% of them reported episodes of calabar swellings, 69.3% history of eyeworm and 10.9% episodes of subcutaneous migration of worms during the last 12 months. pruritus and secondary dermal lesions were freque ... | 1990 | 2339244 |
| filariasis focus due to mansonella ozzardi and mansonella perstans in the amazon federal territory of venezuela. | the presence of filariasis was investigated through knott's method in 40 curripaco indians from communities located on the casiquiare and guainia rivers in the amazon federal territory of venezuela. the results gave an 82.5% prevalence for infections with mansonella perstans and 30% for infections with m. ozzardi (mixed infections with m. perstans). in almost all the patients the parasite load was greater for m. perstans and varied between 1 and 327 microfilariae per ml. this paper presents for ... | 1990 | 2348493 |
| ecdysteroid-like compounds in the serum and urine of african patients infected with loa loa and mansonella perstans microfilariae. | ecdysteroids are compounds related to 20-hydroxyecdysone, the insect moulting hormone. surprisingly, they have been found in serum and urine of patients infected with helminths. in these cases, the substances are assumed to be produced by the parasites and, therefore, might be used as a marker of parasitic infection. thus, we need to know exactly which species, at which developmental stage, can release ecdysteroids in such large quantities that they could be detected in the biological fluids of ... | 1990 | 2389319 |
| steroid and gonadotropin hormone levels in young african women with filarial infection. | serum levels of dehydroepiandrosterone sulfate (dheas), testosterone (t), progesterone (p), estradiol (e2), prolactin (prl), cortisol (f) and gonadotropins (fsh, lh) were analysed by radioimmunoassay for 125 schoolgirls aged 14-16, in a zone of endemic filariasis 3 days after menses. two groups were identified: the infected group in which 38 subjects had circulating loa loa and or mansonella perstans microfilariae as determined by the knott's concentration technique, and the non-infected group ( ... | 1989 | 2533954 |
| [pruritus after taking chloroquine and filariasis]. | a study of relations between pruritus after doses of chloroquine and on the one hand the infection by mansonella perstans (filariasis frequent in some areas but pathogenic) and on the other hand the infection by onchocerca volvulus (filariasis frequent in some other areas but not very pathogenic) was carried out in two villages in ivory coast. it is not possible to show an obvious relation between the presence of pruritus and the two filariasis. | 1989 | 2545369 |
| hypogonadism and ecdysteroid production in loa loa and mansonella perstans filariasis. | possible endocrinological repercussions of infection with loa loa and mansonella perstans filariae were studied in gabonese subjects. microfilaremic males were compared with amicrofilaremic controls. in the infected group 13/105 subjects (12%) presented only abnormally low serum levels of testosterone (less than 4 ng/ml), 25/105 (24%) only abnormally high serum levels of gonadotrophins, fsh (greater than 15 miu/ml) and lh (greater than 20 miu/ml), and 22/105 (21%) presented anomalies in both tes ... | 1989 | 2571252 |
| loa loa and mansonella perstans filariasis in the chaillu mountains, congo: parasitological prevalence. | 1934 bantus and 379 pygmies were investigated for loa loa and mansonella perstans filariasis in 7 villages in the chaillu forest of the congo. bantus were more frequently infected with l. loa than pygmies (18.9% of microfilariae carriers compared with 10.6%). in individuals over 30 years of age, males were more frequently infected than females. microfilarial densities increased until the age of 20 years and then remained stable. parasite load was not significantly different in the two ethnic gro ... | 1989 | 2617607 |
| [32 cases of recurrent localized edema on return from the tropics]. | thirty-two patients have been hospitalised in marseilles since 1979 for recurrent oedema following their return from a tropical region. in spite of strong suspicion, the diagnosis of loa loa was confirmed only five times. one patient was diagnosed with filariasis due to mansonella perstans. the diagnosis of loiasis was usually presumptive and there were often wide variations in the laboratory findings. two patients were thought to have an allergic reaction to diethylcarbamazine. another patient ... | 1989 | 2743518 |
| [the efficacy and tolerance of ivermectin (mectizan) prescribed for the patient with multiple filarial infections (loa loa/onchocerca and/or m. perstans)]. | administration of ivermectin (mectizan) in loa loa filariasis induces a significant decrease of microfilaria load within 3 to 15 days. the excellent tolerability of the drug in patients infested simultaneously by o. volvulus and loa loa allows to use ivermectin in mass treatments in geographical areas where the same patients can be affected by several filariasis. there is almost no efficacy against mansonella perstans. a second administration one month after the first one in loa loa affected pat ... | 1989 | 2743519 |
| haemoparasites of blood donors in calabar. | the study of haemoparasites in our blood donors revealed the following parasite prevalences: microfilaria (mf) of loa loa (1.3%); dipetalonema perstans (15.6%); loa loa and d. perstans (0.2%), plasmodium falciparum (3.3%), plasmodium malariae (1.0%) and a mixture of p. falciparum and p. malariae (0.2%). no trypanosomes were observed in the 480 blood samples screened. there were more cases of d. perstans infection in the 24-30 year age group, indicating their increased exposure frequencies. match ... | 1989 | 2763348 |
| filariasis in the igwun river basin, nigeria: an epidemiological and clinical study with a note on the vectors. | in a cross-sectional, epidemiological and parasitological study of human filariasis, 845 individuals were examined in settlements along the igwun basin, imo state, nigeria. four different filarial nematode species were identified. two hundred and fifty-six (30.3%) of the individuals examined were positive for onchocerca volvulus, 113 (13.4%) for mansonella perstans, 76 (9.6%) for wuchereria bancrofti and 77 (9.1%) for loa loa. microfilarial rates increased with age of individuals and showed a te ... | 1988 | 3041931 |
| nodules in the conjunctiva, bung-eye, and bulge-eye in africa caused by mansonella perstans. | eight patients from uganda, sudan, nigeria, and zaire presented with swelling of the eyelids, proptosis, or conjunctival granulomas. in 5 patients the cause was mansonella perstans; in 1, it was a wuchereria bancrofti-like worm; and in 2, an unidentifiable worm. the morphologic features and histopathologic changes in the conjunctiva and periorbital fat are described and illustrated. | 1988 | 3079314 |
| [filarial multiparasitism in the savannah zone in burkina faso]. | from june 1985 to may 1986, 1.209 consulting patients were examined for filariae in skin and blood. among patients with microfilariae 17% had associations of filarial infections. the multiple infections rate seemed more important in man than woman and increased with age. the results showed that associations were not due to chance only. the frequencies of associations between dipetalonema perstans and onchocerca volvulus at one hand, dipetalonema perstans and wuchereria bancrofti on the other han ... | 1987 | 3323769 |
| a rapid, simple method for isolation of viable microfilariae. | we describe a rapid, efficient method for purifying loa loa microfilariae from blood that does not affect their longterm motility. it is based on the separation of microfilariae on a performed discontinuous percoll gradient, followed by filtration on cellulose ester membranes. the parasite population, after isolation, is close to 100% pure, 100% viable and remains motile for at least 20 days. recovery from original blood samples is typically around 90%. the method, because of its simplicity and ... | 1986 | 3456213 |
| density gradient separation of loa loa and dipetalonema perstans microfilariae from infected patients. | | 1986 | 3459401 |
| lack of evidence for transplacental transfer of microfilarial antigens in filariasis due to loa loa and mansonella perstans. | the transplacental transfer of microfilarial antigens could induce prenatal tolerance, or conversely resistance, which would influence the development of filariasis after birth. the passage across the placenta of immunogens constitutive of a crude microfilarial loa loa extract has been investigated by dosing specific igg, igm and ige antibodies in 92 maternal and corresponding cord blood samples, igg levels were shown to be identical in about half of the mother-cord paired samples while in the o ... | 1986 | 3462890 |
| treatment of recurrent, filarial, calabar-type oedema with mebendazole. | a case of recurrent, severe filarial calabar-type oedema, causing acute discomfort in the patient, is described in a male caucasian. an adult loa loa worm but no microfilariae were present while dipetalonema perstans microfilaraemia was extremely low. treatment with mebendazole (1.5 g per day) for 21 days led to the disappearance of the clinical symptoms and microfilariae, and to a decrease of eosinophilia to normal values. | 1986 | 3463005 |
| specific serodiagnosis with adult onchocerca volvulus antigen in an enzyme-linked immunosorbent assay. | the cross-reactivity of the blood from onchocerciasis, loiasis, and dipetalonemiasis was tested by a micro-elisa technique, utilizing adult onchocerca volvulus antigen and blood samples taken on filter paper. the average elisa values (od at 500 nm) were as follows: 0.58 in persons with o. volvulus microfilariae (n = 81), 0.49 in microfilariae-negatives from the same endemic area (n = 39), 0.15 in dipetalonemiasis (n = 27), and 0.25 in loiasis (n = 12), while those of 65 dipetalonema perstans-neg ... | 1987 | 3469920 |
| the use of mebendazole in the treatment of filariases due to loa loa and mansonella perstans. | patients harbouring loa loa and/or mansonella perstans infections, or presenting typical filarial symptomatology, were treated with mebendazole, a drug shown to be efficient in other parasitoses. of the patients who took the drug during 21 days and provided regular blood samples, 84% showed a significant decrease in their l. loa microfilaraemia and 100% in their m. perstans microfilaraemia, with a concomitant improvement in the clinical manifestations and no side reaction to drug administration. ... | 1987 | 3478004 |
| filariasis due to loa loa and mansonella perstans: distribution in the region of okondja, haut-ogooué province, gabon, with parasitological and serological follow-up over one year. | the prevalence of loa loa and mansonella perstans filariasis has been determined in 6 rural villages in eastern gabon. between 18.9 and 27.2% of people carry l. loa microfilariae with an overall microfilarial rate of 25.1%. the microfilarial rate for m. perstans was more variable, between 33.3 and 62.2% (average 49.1%). no significant difference was seen in the microfilarial rate with age over 15 years for either parasite, but men were infected more frequently than women. anti-l. loa antibody ti ... | 1987 | 3479854 |
| cell adherence to microfilariae of onchocerca volvulus: a comparative study. | the conditions were examined for in vitro antibody-mediated adherence of granulocytes to microfilariae of onchocera volvulus and dirofilaria immitis. reactivity in human sera from patients in endemic foci in sudan was specific for o. volvulus and no reactions were observed with heterologous onchocerca species or with mansonella perstans. microfilariae from skin, nodules or adult female worms were satisfactory targets for cell adherence, and the cells involved were almost exclusively eosinophils. ... | 1987 | 3595320 |
| mansonella perstans microfilaraemia among the bori community in the niger delta area of nigeria. | night and day blood surveys for filariasis were conducted among inhabitants of bori local government area in the niger delta area of nigeria between october 1983 and july 1984. of the 1674 persons examined 47.0% had microfilaraemia. the infections were due to mansonella perstans only. the prevalence of the infection was higher among males (51.8%) than among females (42.4%), and increased with age in both sexes. eight persons were found with elephantoid scrotums and all had microfilariae of m. pe ... | 1986 | 3632101 |
| adult mansonella perstans in the abdominal cavity in nine africans. | adult mansonella perstans infected the abdominal cavity of nine patients seen at karawa hospital in the ubangi territory of zaire. in four patients the worms were removed at laparotomy, and in the other five they were removed at autopsy. twelve adult worms were identified in the nine patients. none of the worms caused symptoms or contributed to the patient's death. worms were in the hernial sac in three patients, and one each was in connective tissue beside a reactive mesenteric lymph node, in p ... | 1987 | 3688309 |
| extended cryopreservation of loa loa and mansonella perstans microfilariae from human peripheral blood. | | 1986 | 3810789 |
| onchocerciasis in southwestern sudan: parasitological and clinical characteristics. | parasitological and clinical observations were made on residents of pongo nuer, a village in the province of bahr el ghazal, southwestern sudan. of 202 skin biopsies, 189 (94%) were positive for microfilariae of onchocerca volvulus. nodules were most common around the pelvic girdle and rare on the limbs or head. microfilarial intensities, ranging up to 1,094 mf/mg of skin, were highest at the iliac crest and shoulder; they increased rapidly in childhood but then appeared to reach a plateau maint ... | 1987 | 3826496 |
| seroepidemiological investigations of onchocerciasis in a hyperendemic area of west africa. | immunological study of individuals (aged 4 to 70 years) living in an area of mali hyperendemic for onchocerciasis revealed an 83% prevalence of skin microfilariae (mf). microfilariae counts from skin snips were highly age-dependent. screening for concomitant helminth infections showed a low prevalence of hookworms and mansonella (dipetalonema) perstans, but neither schistosomiasis nor bancroftian filariasis. immunological results revealed strong correlation between radioallergosorbent test (rast ... | 1985 | 3898885 |
| specificity of surface molecules of adult brugia parasites: cross-reactivity with antibody from wuchereria, onchocerca and other human filarial infections. | the extent of structural and immunological similarity between surface antigens of three species of brugia filarial parasites was studied by lodogen-mediated surface labelling of adult worms of b. malayi, b. pahangi and b. timori. the close homology and cross-reactivity between these antigens reported in previous surface labelling studies with bolton-hunter reagent, was verified in this system. the surface antigens of adult b. pahangi are also recognised by antibody from patients with wuchereria ... | 1985 | 3911361 |
| first finding of dipetalonema perstans in colombia. filariasis in colombia. 3. | | 1973 | 4735086 |
| filariasis: dipetalonema perstans infections in rhodesia. | | 1971 | 5103154 |
| further observations on dipetalonema perstans. | | 1971 | 5165107 |
| [evaluation of 2 immunological tests (skin test and complement fixation test) for the detection of filariasis in populations of upper volta where wuchereria bancrofti, onchocercă volvulus and dipetalonema perstans occur together]. | | 1969 | 5307597 |
| [contribution to the study of filariasis existing in angola. 1-onchocerca volvulus. 2.-loa loa. 3.-dipetalonema perstans]. | | 1967 | 5629059 |
| [symptomatology of filariosis due to dipetalonema perstans]. | | 1965 | 5898897 |
| [contribution to the study of existent forms of filariasis in angola. 1. onchocerca volvulus. 2. loa loa. 3. dipetalonema perstans]. | | 1966 | 6011046 |
| infections with dipetalonema perstans and mansonella ozzardi in the aboriginal indians of guyana. | | 1967 | 6069567 |
| the public health importance of african trypanosomiasis in north east zambia. | a survey for sleeping sickness (trypanosoma brucei rhodesiense) in villages in the northern luangwa valley found a point prevalence of parasitaemia of 5.8 per thousand; the incidence of the disease was estimated at about 1% per annum. none of the cases found had sought treatment and pre-symptomatic cases may represent an important reservoir of infection to others. other frequent parasitemias in the population included malaria (p. falciparum, p. malariae and p. ovale) which is hyperendemic and di ... | 1984 | 6335610 |
| filariasis in colombia: prevalence and distribution of mansonella ozzardi and mansonella (=dipetalonema) perstans infections in the comisaría del guainía. | a survey was conducted to determine the prevalence and distribution of filarial infections among the inhabitants of comisaría. approximately 25% of the 604 individuals bled (knott's sample) harbored microfilariae; more men (29.7%) were infected than women (23.7%); approximately 6% were infected with mansonella (=dipetalonema) perstans, 13% with mansonella ozzardi, and 7% with both filariae. mansonella ozzardi was more common in men than in women, and its prevalence increased with age in both sex ... | 1983 | 6340542 |
| immune response in a symptomatic case of tetrapetalonema perstans infection. | the immune response in a 49-year-old italian nun affected by tetrapetalomena (dipetalonema) perstans following an eight-year stay in cameroon is reported. on admission the patient presented with polyarthralgia, conjunctival pruritus and irritation, oedema of the limbs, generalized itching, hypereosinophilia and low total ige titre. during diethylcarbamazine treatment and, subsequently, during mebendazole administration, an exacerbation of her symptoms was observed. at the same time, activation o ... | 1984 | 6541380 |
| lymphadenitis caused by loa loa. | the world health organization's collaborating centre for the histopathology of filarial diseases of man contains specimens of lymph node from 13 patients infected by the filarial nematode loa loa. ten of these nodes have distinctive microscopic features characterized by distended sinuses that contain histiocytes and eosinophils and by atrophy of lymphoid follicles. less striking features include fibrosis of capsule and trabeculae, dilated lymphatic vessels of capsule and medulla, and inflammator ... | 1984 | 6587787 |
| [clinical observations on treatment of dipetalonema perstans and loa loa filariasis with furapyrimidone]. | | 1984 | 6590137 |
| pleural effusion associated with dipetalonema perstans (acanthocheilonema perstans). | | 1983 | 6681622 |
| dipetalonema perstans in a patient with chronic lymphoedema. case report. | | 1983 | 6684545 |
| treatment of dipetalonema perstans infections with mebendazole. | | 1983 | 6684816 |
| report of an imported case of dipetalonema perstans in japan. | a case of dipetalonema perstans infection in a 34-year-old japanese male is presented and from his past history there is no doubt that he obtained the infection in zaire, africa. the morphology and periodicity of the microfilaria were studied in detail and the clinical manifestations of the infection documented. this is the first report of imported d. perstans infection in japan. | 1983 | 6686343 |
| filariasis in amerindians of western guyana with observations on transmission of mansonella ozzardi by a simulium species of the amazonicum group. | in order to conduct experimental infection studies of mansonella ozzardi in local haematophagous diptera, volunteers infected with this parasite were identified during a microfilaria survey in four amerindian villages in the pakaraima mountains of western guyana, near the brazilian border. m. ozzardi microfilariae were detected in thick blood smears from 8-21% of persons examined. they were also found in skin snips from 8/73 persons all of whom were positive by blood smear examination. no onchoc ... | 1982 | 6761913 |
| the successful treatment of dipetalonema perstans filariasis with mebendazole. | | 1982 | 6891576 |
| [prevalence of human filariasis with microfilaremia in gabon]. | direct examinations of blood and serologic tests were used to assess the intensity of the filarial endemicity in gabon. both methods revealed that the prevalence of filariasis with microfilaremia (loa loa and dipetalonema perstans) is presently very elevated. the areas that are more involved are the forest regions as well as the highest ones (ogoue, lolo, ngounie). women appeared to be suffering from loasis to a lesser extent than men. on the other hand, the prevalence was identical for dipetalo ... | 1980 | 6936082 |
| [serodiagnosis of onchocerciasis using micro-elisa. study of 450 sera and comparison with indirect immunofluorescence]. | the microtest elisa has been used for human onchocerciasis serological study. the antigens employed were adult onchocerca volvulus extracts, collected from dissected nodules, delipidized and cleared from human proteins by affinity chromatography. under the circumstances, the positivity limit of the test seems excellent (maximum )d: 0,23) defined with 171 negative sera, 66 of them taken from africans. specificity controls were studied with 56 heterologous sera; cross-reactions occurred with hydat ... | 1980 | 7006842 |
| filariasis in colombia: presence of dipetalonema perstans in the comisaría del guainía. | examination of 75 blood samples (knott preparation) collected in puerto inŕida, coco, and pajuil, in the comisaría del guainía, colombia, disclosed 26 microfilaria carriers. eighteen persons harbored only mansonella ozzardi microfilariae, three were infected with m. ozzardi and dipetalonema perstans and five harbored only d. perstans. m. ozzardi infections were found in whites, and in indians belonging to the curripaco, puinave, tukano, guanano and saliva tribes, but d. perstans was found only i ... | 1982 | 7044161 |
| evaluation of the diethylcarbamazine provocative test in the diagnosis of wuchereria bancrofti infections in the nigerian savanna and the effects on dipetalonema perstans. | large scale filariasis surveys in rural areas for microfilaraemia, especially of periodic types such as wuchereria bancrofti are known to cause considerable administrative, technical and social problems. the present investigation was carried out in the population of two villages in the malumfashi district of the northern nigerian savanna. from the survey results, the sensitivity and specificity of two techniques-day-time diethylcarbamazine (dec) provocative test by blood smear and concentration, ... | 1982 | 7051456 |
| miniature anion-exchange/centrifugation technique for the diagnosis of microfilaraemia in the field. | the miniature anion-exchange/centrifugation (aec) technique, developed originally for the detection of low parasitaemias in laboratory rodents, was adapted to field use for the diagnosis of trypanosomiasis in man in africa and was tested in the gambia. during this field study it was found that microfilariae of dipetalonema perstans could also pass through the anion exchange column and appear in the centrifugate as 'medusa heads'. one locality-group (mansafa bolon) showed a generally higher preva ... | 1980 | 7192021 |
| glycolipid antigens of adult o. volvulus reactive with patients sera from onchocerciasis endemic areas in cameroon. | lipid extraction of adult o. volvulus worms using a chloroform/methanol/water mixture yielded 10 lipid fractions of which 8 were demonstrated by the orcinol reagent to be glycolipids. in tlc, two of these lipid fractions had mobilities similar to cholesterol and cholesterol ester (rf.: 0.95, 0.86) whereas two others migrated as sphingomyelin and lecithin (rf.: 0.40, 0.35) respectively. other components migrated at intermediate positions. the glycolipids were immunologically active and reacted wi ... | 1994 | 7604752 |
| immunodiagnostic studies on onchocerca volvulus and mansonella perstans infections using a recombinant 33 kda o. volvulus protein (ov33). | a total detergent-soluble extract of adult female onchocerca volvulus (ovag) and a recombinant o. volvulus protein (ov33) linked to glutathione-s-transferase (gst) were compared with regard to their serodiagnostic suitability for differentiating between o. volvulus and mansonella perstans infections in a region endemic for both filarial worms in western uganda. using ovag in an enzyme-linked immunosorbent assay (elisa), 98.8% sensitivity was obtained examining 84 o. volvulus microfilariae (mf) c ... | 1995 | 7747307 |
| prevalence of l. loa and m. perstans filariasis in southern cameroon. | the prevalence and microfilarial density of loa loa and mansonella perstans filariasis have been determined in three successive surveys in one village in the dense rainforest of southern cameroon. the prevalence of l. loa microfilaraemia was 30.9% with a geometric mean density of 81.4 microfilariae/30 microliter blood. there was no difference between men and women. the prevalence increased with age from 14.5% at 0-9 years to 52.9% at 40-49 years, whereafter it stablized. the microfilarial densit ... | 1995 | 7747326 |
| the status and consequences of mansonella perstans infection in calabar, nigeria. | two aetiological studies on filariasis were conducted from january to june, 1993. in the first study, 690 blood samples clinically diagnosed of filariasis were examined. in the other, 150 blood samples and 150 skin snips collected from the same group of eye clinic patients clinically diagnosed of filariasis were investigated. of the 690 subjects, 108 (15.64%) had filariasis with m. perstans and loa loa accounting for 98.15 and 1.85% of positive cases respectively. among the eye clinic patients, ... | 1995 | 7796752 |
| loa loa antigen detection by elisa: a new approach to diagnosis. | circulating immune complexes associated loa loa antigen in sera of 27 subjects with loa loa (13 'occult' and 14 microfilaraemic infections) was detected with polyethylene glycol enzyme linked immunosorbent assay (peg-elisa) in a controlled study. loa loa antigen was detected in all 27 sera. there was no correlation between antigen levels and microfilaraemia density. cross reaction with mansonella perstans was found. although this serological test is able to detect all loa loa infections, its use ... | 1995 | 7796770 |
| studies on filariasis in bauchi state, nigeria. ii. the prevalence of human filariasis in darazo local government area. | in an eleven months study of eleven communities of darazo local government area of bauchi state, northern nigeria, 293 (21.7) of 1,349 persons examined harboured various filarial parasites. of the sampled population, 18%, 1.9%, 1.6%, 0.5% and 0.2% had microfilariae of onchocerca volvulus, wuchereria bancrofti, mansonella perstans, m. streptocerca and loa loa respectively. of the 637 females examined, 90 (14.1%) were infected, while 203 (23.5%) of 712 males sampled had filarial infections. the hi ... | 1994 | 7812311 |
| high levels of parasite-specific igg4 in the absence of microfilaremia in loa loa infection. | the specificity of igg4 as marker of infection has been investigated. the study was based on two well defined clinical groups: amicrofilaremic individuals with verified ocular passage of adult worms of loa loa, and microfilaremic patients. both groups were compared to africans not exposed to loiasis infection and to europeans. the study revealed that there is no significant difference in the level of parasite-specific igg4 between individuals with occult infection (i.e. amicrofilaraemic, but inf ... | 1994 | 7899797 |
| the status of human filariasis in north-western zone of bauchi state, nigeria. | in a nine-months epidemiological study to assess the status of human filariasis in north-western zone of bauchi state, nigeria, 3.5% prevalence was recorded in 8 communities. of the 1536 persons examined, 54 were microfilaremic for three blood-dwelling filarial parasites. 22 (1.4%) were positive for mansonella perstans, 10 (0.7%) and 22 (1.4%) had loa loa and wuchereria bancrofti respectively. w. bancrofti showed low microfilaremia in females of reproductive age with high degree of heterogeneity ... | 1994 | 8087153 |
| efficacy of ivermectin in the treatment of concomitant mansonella perstans infections in onchocerciasis patients. | as part of an ivermectin dose-ranging study of onchocerciasis patients in togo, 55 onchocerciasis patients with concomitant mansonelliasis received single oral doses either of ivermectin (100 to 200 micrograms/kg body weight) or placebo. as expected, onchocerca volvulus microfilariae in the skin were greatly reduced in number soon after drug treatment, but microfilariae of mansonella perstans reacted differently. microfilarial densities of m. perstans were assessed with a filtration technique bo ... | 1993 | 8337736 |
| treatment failure of a single high dose of ivermectin for mansonella perstans filariasis. | infections with mansonella perstans are common in certain parts of africa and south america. there is no standard treatment at present. we evaluated the effect of a single high dose of ivermectin (600 micrograms/kg) on microfilaraemia in 7 consecutive patients. no decrease in microfilarial counts could be demonstrated after a follow-up period of 7-56 d. | 1993 | 8465410 |
| prevalence of mansonella perstans in western uganda and its detection using the qbc-fluorescence method. | the prevalence of the apathogenic filaria mansonella perstans was studied in four parishes in western uganda as part of an onchocerciasis control programme to avoid futile treatment. blood samples from 1543 persons aged over 14 years from 19 villages were examined for the presence of microfilariae using a modified knott method. the prevalence of microfilaraemic persons ranged between the parishes from 39% (95% ci 35.9-42.0%) to 81% (95% ci 76.2-84.8%). with exception of single microfilariae of o ... | 1996 | 8574245 |
| filariasis in the kaiyamba chiefdom, moyamba district sierra leone: an epidemiological and clinical study. | in a cross-sectional epidemiological and clinical study of human filariasis, 630 individuals were examined for onchocerca volvulus, wuchereria bancrofti and mansonella perstans infections in five communities in the kaiyamba chiefdom, moyamba district, sierra leone. the overall prevalence of o. volvulus infection in males 144(39.1%) and females 94(35.9%) was not significantly different and the sex prevalence rate between communities was also not significant (g = 3, d.f. = 4, p > 0.05). prevalence ... | 1996 | 8668763 |
| an unexpected guest in follicular fluid. | parasitic infection as the only or concomitant cause of infertility in caucasian women is rare. a parasitic infection may also present itself quite unexpectedly as a coincidental finding as shown with this case report. moving microfilariae of mansonella perstans were found in the aspirated follicular fluid of a patient who underwent in-vitro fertilization (ivf) with embryo transfer because of tubal pathology due to chlamydia trachomatis. the patient also appeared to have a schistosoma infection. ... | 1996 | 8671260 |
| markers of loa loa infection in permanent residents of a loiasis endemic area of gabon. | different markers of infection were analyzed in 56 permanent residents of a loa loa endemic village in gabon. the population was divided into those with parasitological evidence of l. loa infection and those with no history of loiasis over the period of observation (c. 5 years). 26.7% of villagers had l. loa microfilariae, 33.9% had an ocular passage of an adult worm, and 17.8% had calabar oedema. several other clinical symptoms were present in both groups of individuals, but none was considered ... | 1996 | 8761565 |
| species-specific sequence in the repeat 3 region of the gene encoding a putative loa loa allergen: a diagnostic tool for occult loiasis. | a polymerase chain reaction (pcr)-based method to detect loa loa dna in the blood lysate of infected individuals is described. a set of primers was designed to amplify the repeat 3 sequence (15r3) of the gene encoding a putative l. loa allergen. the qualitative pcr was carried out using blood lysates from subjects from an l. loaendemic area of gabon where loiasis exists sympatrically with mansonella perstans, and from individuals from a loiasis-free area in togo infected concomitantly with m. pe ... | 1997 | 9063362 |
| molecular phylogenetic studies on filarial parasites based on 5s ribosomal spacer sequences. | this paper is the first large-scale molecular phylogenetic study on filarial parasites (family onchocercidae) which includes 16 species of 6 genera: brugia beaveri ash et little, 1962, b. buckleyi dissanaike et paramananthan, 1961; b. malayi (brug, 1927) buckley, 1960; b. pahangi (buckley et edeson, 1956) buckley, 1960; b. patei (buckley, nelson et heisch, 1958) buckley, 1960; b. timori partono et al, 1977; wuchereria bancrofti (cobbold, 1877) seurat, 1921: w. kalimantani palmieri. purnomo, denn ... | 1994 | 9140481 |
| the diverse expression of immunity in humans at distinct states of onchocerca volvulus infection. | this study examined the development and persistence of immunity in humans presenting defined states of onchocerca volvulus infection, i.e. in exposed endemic control individuals without microfilaridermia and clinical disease, in patients with patent or post-patent onchocerciasis, and in patients concurrently infected with mansonella perstans. onchocerca volvulus antigen (ovag)-specific cellular reactivity was significantly diminished in microfilariae (mf)-positive patients, while the highest rea ... | 1997 | 9176114 |
| albendazole for the treatment of mansonella perstans filariasis. | | 1997 | 9196776 |