| phylogenetic inferences and taxonomic consequences of 16s ribosomal dna sequence comparison of chromohalobacter marismortui, volcaniella eurihalina, and deleya salina and reclassification of v. eurihalina as halomonas eurihalina comb. nov. | the phylogenetic positions of the moderately halophilic bacteria chromohalobacter marismortui, volcaniella eurihalina, and deleya salina were determined by pcr amplification of rrna genes and direct sequencing. the resulting data were compared with data for other bacteria obtained from 16s rrna sequence databases. c. marismortui, v. eurihalina, and d. salina clustered phylogenetically within the gamma subclass of the proteobacteria and are closely related to other species on the halomonas-deleya ... | 1995 | 7547290 |
| characterization of the basic replicon of pcm1, a narrow-host-range plasmid from the moderate halophile chromohalobacter marismortui. | the moderately halophilic bacterium chromohalobacter marismortui contains a 17.5-kb narrow-host-range plasmid, pcm1, which shows interesting properties for the development of cloning vectors for the genetic manipulation of this important group of extremophiles. plasmid pcm1 can stably replicate and is maintained in most gram-negative moderate halophiles tested. the replication origin has been identified and sequenced, and the minimal pcm1 replicon has been localized to a 1,600-bp region which in ... | 1995 | 7768853 |
| influence of salt concentration on the susceptibility of moderately halophilic bacteria to antimicrobials and its potential use for genetic transfer studies. | the influence of salinity on the susceptibility of 13 moderately halophilic collection strains belonging to the genera chromohalobacter, deleya, halomonas, vibrio, and volcaniella to 10 common antimicrobials has been studied. three different patterns of tolerance were found when salinity was varied from 10 to 1% (wt/vol) total salts in the testing media. the first one included the responses to ampicillin and rifampicin, where only minimal effects on the susceptibility were found. all moderate ha ... | 1995 | 8528008 |
| construction of novel shuttle vectors for use between moderately halophilic bacteria and escherichia coli. | shuttle vectors useful for the genetic manipulation of several moderately halophilic bacteria have been constructed. these vectors are based on the minimal replicon of pcm1, a cryptic plasmid from chromohalobacter marismortui, combined with the useful properties of puc18 plasmid (i.e., small size, high copy number, multiple cloning sites, lacz fragment), as well as with the trimethoprim resistance gene as a selection marker for moderate halophiles. these vectors can be efficiently transferred by ... | 1995 | 8825368 |
| analysis of the genome of the gram-negative moderate halophiles halomonas and chromohalobacter by using pulsed-field gel electrophoresis. | the genomes of 11 moderately halophilic bacteria belonging to the genera halomonas and chromohalobacter have been analyzed by restriction endonuclease digestion and pulsed-field gel electrophoresis (pfge). by using the infrequently cutting restriction endonucleases spei and swai, highly characteristic fingerprintings were obtained for each of the isolates studied. on the basis of the lengths of the spei and swai fragments, separated by pfge, the genome size of the 11 strains studied was estimate ... | 1998 | 9827333 |
| characterization of the genes for the biosynthesis of the compatible solute ectoine in the moderately halophilic bacterium halomonas elongata dsm 3043. | the ectoine synthesis genes of the moderately halophilic bacterium halomonas elongata dsm 3043 have been precisely located in a 2.8-kb ecori region of a cosmid clone previously isolated (canovas, d., vargas, c., iglesias-guerra, f., csonka, l. n., rhodes, d., ventosa, a., nieto, j. j.: isolation and characterization of salt-sensitive mutants of the moderate halophile halomonas elongata and cloning of the ectoine synthesis genes. j. biol. chem. 272, 25794-25801, 1997). this region was sequenced a ... | 1998 | 9924816 |
| halomonas magadii sp. nov., a new member of the genus halomonas, isolated from a soda lake of the east african rift valley. | a number of novel alkaliphilic organotrophic bacteria have been isolated from several saline and alkaline east african soda lakes. the new isolates grow at ph values between 7.0 and 11.0, with ph optima for growth between 9.0 and 10.0. growth occurs at total salts concentration between 0% and 20% (w/v) with optimum at 0%-7% (w/v). phylogenetic analyses based on 16s rdna sequence comparison indicate that these isolates are related (>96% similarity) to members of the halomonadaceae within the gamm ... | 2000 | 10741837 |
| organophosphonate metabolism by a moderately halophilic bacterial isolate. | a gram-negative halophile isolated from soil beneath a road gritting salt pile grew optimally at 10% (w/v) nacl and was shown most likely to be chromohalobacter marismortui or pseudomonas beijerinckii on the basis of 16s rrna analysis. the strain utilised phosphonoacetate, 2-aminoethyl-, 3-aminopropyl-, 4-aminobutyl-, methyl- and ethyl-phosphonates as phosphorus sources for growth. differences were observed in the growth rate on different phosphonates and the range of phosphonates utilised at el ... | 2000 | 10802166 |
| transfer of halomonas canadensis and halomonas israelensis to the genus chromohalobacter as chromohalobacter canadensis comb. nov. and chromohalobacter israelensis comb. nov.. | 16s rrna gene sequence comparisons and dna-dna hybridization data support the conclusion that two species previously described as members of the genus halomonas, halomonas israelensis and halomonas canadensis, should be placed in the genus chromohalobacter. both h. israelensis atcc 43985t (= ba1t) and h. canadensis atcc 43984t (= nrcc 41227t) have been used extensively for physiological studies for many years; nevertheless, they were not named and classified taxonomically until quite recently. t ... | 2001 | 11491344 |
| chromohalobacter salexigens sp. nov., a moderately halophilic species that includes halomonas elongata dsm 3043 and atcc 33174. | two strains that were originally isolated and characterized as members of the moderately halophilic species halomonas elongata, strains dsm 3043 (= 1h11) and atcc 33174 (= 1h15), were studied in detail. their complete 16s rrna sequences were determined and, when compared to sequences available from the databases, they showed a close phylogenetic relationship to chromohalobacter marismortui. in addition, dna-dna hybridization experiments showed that both strains are members of the same species, b ... | 2001 | 11491346 |
| phylogeny of the family halomonadaceae based on 23s and 165 rdna sequence analyses. | in this study, we have evaluated the phylogenetic status of the family halomonadaceae, which consists of the genera halomonas, chromohalobacter and zymobacter, by comparative 23s and 16s rdna analyses. the genus halomonas illustrates very well a situation that occurs often in bacterial taxonomy. the use of phylogenetic tools has permitted the grouping of several genera and species believed to be unrelated according to conventional taxonomic techniques. in addition, the number of species of the g ... | 2002 | 11837309 |
| utilisation of aminomethane sulfonate by chromohalobacter marismortui vh1. | chromohalobacter marismortui vh1 was screened for its ability to utilise organosulfonate compounds at a range of nacl concentrations. only aminomethane sulfonate, of seven sulfonates tested, was utilised. length of lag phase during growth on aminomethane sulfonate, as either nitrogen and/or sulfur source, increased with increasing nacl concentration. cell yields increased linearly with increasing aminomethane sulfonate concentration up to 5 mm. resting cells pregrown on aminomethane sulfonate as ... | 2002 | 11886750 |
| polyamine distribution patterns within the families aeromonadaceae, vibrionaceae, pasteurellaceae, and halomonadaceae, and related genera of the gamma subclass of the proteobacteria. | polyamines of the four families and the five related genera within the gamma subclass of the class proteobacteria were analyzed by hplc with the objective of developing a chemotaxonomic system. the production of putrescine, diaminopropane, cadaverine, and agmatine are not exactly correlated to the phylogenetic genospecies within 36 strains of the genus aeromonas (the family aeromonadaceae) lacking in triamines. the occurrence of norspermidine was limited but not ubiquitous within the family vibr ... | 1997 | 12501353 |
| diversity of moderately halophilic bacteria producing extracellular hydrolytic enzymes. | the aim of this study was to determine the diversity of moderately halophilic bacteria with hydrolase activities. | 2003 | 12534822 |
| saccharospirillum impatiens gen. nov., sp. nov., a novel gamma-proteobacterium isolated from hypersaline ekho lake (east antarctica). | five gram-negative, motile, aerobic to microaerophilic spirilla were isolated from various depths of the hypersaline, heliothermal and meromictic ekho lake (east antarctica). the strains are oxidase- and catalase-positive, metabolize a variety of sugars and carboxylic acids and have an absolute requirement for sodium ions. the predominant fatty acids of the organisms are c(16 : 1)omega7c, c(16 : 0) and c(18 : 1)omega7c, with c(10 : 1))3-oh, c(10 : 0) 3-oh, c(12 : 0) 3-oh, c(14 : 1)3-oh, c(14 : 0 ... | 2003 | 12807182 |
| the high salt requirement of the moderate halophile chromohalobacter salexigens dsm3043 can be met not only by nacl but by other ions. | the growth rate of chromohalobacter salexigens dsm 3043 can be stimulated in media containing 0.3 m nacl by a 0.7 m concentration of other salts of na+, k+, rb+, or nh4+, cl-, br-, no3-, or so4(2-) ions. to our knowledge, growth rate stimulation by a general high ion concentration has not been reported for any organism previously. | 2003 | 14532102 |
| highly efficient renaturation of beta-lactamase isolated from moderately halophilic bacteria. | most, if not all, beta-lactamases reported to date are irreversibly denatured at 60-70 degrees c. here, we found that a halophilic beta-lactamase from the moderately halophilic bacterium chromohalobacter sp. 560 was highly stable against heat inactivation: it retained approximately 75% of its activity after boiling for 5 min in the presence of 0.2 m nacl, suggesting that the protein either incompletely denatures during the boiling process or readily renatures upon cooling to the assay temperatur ... | 2004 | 14759507 |
| major outer membrane proteins in moderately halophilic eubacteria of genera chromohalobacter and halomonas. | outer and inner membrane fractions of chromohalobacter marismortui and halomonas elongata were isolated by differential detergent solubilization, and profiles of membrane proteins, especially major outer membrane proteins, were analyzed. these type strains possessed one extremely abundant outer membrane protein which showed similarity in amino-terminal amino acid sequence with the outer membrane porin proteins in other gram-negative bacteria. three halophilic eubacterial strains isolated from sa ... | 2004 | 15162397 |
| genetic tools for the manipulation of moderately halophilic bacteria of the family halomonadaceae. | moderately halophilic bacteria of the family halomonadaceae (halomonas, chromohalobacter, and zymobacter) have promising applications in biotechnology as a source of compatible solutes (stabilizers of biomolecules and cells), salt-tolerant enzymes, biosurfactants, and extracellular polysaccharides, among other products. in addition, they offer a number of advantages to be used as cell factories, alternative to conventional prokaryotic hosts like e. coli or bacillus, for the production of recombi ... | 2004 | 15269425 |
| how to be moderately halophilic with broad salt tolerance: clues from the genome of chromohalobacter salexigens. | we analyzed the amino acid composition of different categories of proteins of the moderately halophilic bacterium chromohalobacter salexigens, as deduced from its genome sequence. comparison with non-halophilic representatives of the gamma-proteobacteria (escherichia coli, pseudomonas aeruginosa, vibrio cholerae) shows only a slight excess of acidic residues in the cytoplasmic proteins, and no significant differences were found in the acidity of membrane-bound proteins. in contrast, a very prono ... | 2005 | 15902510 |
| contribution of chemical changes in membrane lipids to the osmoadaptation of the halophilic bacterium chromohalobacter salexigens. | the long-term response of the broad-salt growing halophile chromohalobacter salexigens dsm 3043t to salt stress has been investigated with respect to adaptive changes in membrane lipid composition. this study included the wild-type and three salt-sensitive, ectoine-deficient strains: chr62 (ecta::tn1732, unable to grow above 0.75 m nacl), chr63 (ectc::tn1732, unable to grow above 1.5 m nacl), and chr64, which was able to grow in minimal medium m63 up to 2.5 m nacl, but its growth was slower than ... | 2005 | 16156114 |
| ectoines as compatible solutes and carbon and energy sources for the halophilic bacterium chromohalobacter salexigens. | to investigate the catabolism of ectoine and hydroxyectoine, which are the major compatible solutes synthesized by chromohalobacter salexigens. | 2006 | 16405689 |
| osmoprotection of salmonella enterica serovar typhimurium by ngamma-acetyldiaminobutyrate, the precursor of the compatible solute ectoine. | n(gamma)-acetyl-2,4-diaminobutyrate (nada), the precursor of the compatible solute ectoine, was shown to function as an osmoprotectant for the non-halophilic bacterium salmonella enterica serovar typhimurium. the addition of nada-containing extracts of an ectoine synthase mutant of the broad salt-growing halophile chromohalobacter salexigens dsm 3043(t) could alleviate the inhibitory effects of high salinity in s. enterica, which lacks the ectoine biosynthetic pathway. nada, purified from extrac ... | 2006 | 16469465 |
| molecular microbial diversity of a soil sample and detection of ammonia oxidizers from cape evans, mcmurdo dry valley, antarctica. | the aim of our study was to estimate the uncultured eubacterial diversity of a soil sample collected below a dead seal, cape evans, mcmurdo, antarctica by an ssu rdna gene library approach. our study by sequencing of clones from ssu rdna gene library approach revealed high diversity in the soil sample from antarctica. more than 50% of clones showed homology to cytophaga-flavobacterium-bacteroides group; sequences also belonged to alpha, beta, gamma proteobacteria, thermus-deinococcus and high gc ... | 2007 | 16517136 |
| biodiversity of moderately halophilic bacteria in hypersaline habitats in egypt. | screening bacteria from different saline environments in alexandria. egypt, lead to the isolation of 76 gram-negative and 14 gram-positive moderately halophilic bacteria. the isolates were characterized taxonomically for a total of 155 features. these results were analyzed by numerical techniques using simple matching coefficient (ssm) and the clustering was achieved by the unweighed pair-group method of association (upgma). at 75% similarity level the gram-negative bacteria were clustered in 7 ... | 2006 | 16778349 |
| cloning and characterization of the genes for biosynthesis of the compatible solute ectoine in the moderately halophilic bacterium halobacillus dabanensis d-8(t). | a 11.2-kb fragment containing the ectabc genes of the biosynthetic pathway of ectoine from the gram-positive, moderately halophilic bacterium halobacillus dabanensis d-8(t) was obtained by inverse polymerase chain reaction. subsequently, the entire ectabc cluster was cloned and analyzed. it revealed that the intergenic regions of the ectabc genes from h. dabanensis d-8(t) are more tightly spaced than those of chromohalobacter salexigens, halomonas elongata, marinococcus halophilus, and salibacil ... | 2006 | 16874550 |
| phylogenetic analysis of a biofilm bacterial population in a water pipeline in the gulf of mexico. | the aim of this study was to assess the bacterial diversity associated with a corrosive biofilm in a steel pipeline from the gulf of mexico used to inject marine water into the oil reservoir. several aerobic and heterotrophic bacteria were isolated and identified by 16s rrna gene sequence analysis. metagenomic dna was also extracted to perform a denaturing gradient gel electrophoresis analysis of ribosomal genes and to construct a 16s rrna gene metagenomic library. denaturing gradient gel electr ... | 2006 | 16958915 |
| continuous synthesis and excretion of the compatible solute ectoine by a transgenic, nonhalophilic bacterium. | the compatible solute 1,4,5,6-tetrahydro-2-methyl-4-pyrimidinecarboxylic acid (ectoine) acts in microorganisms as an osmotic counterweight against halostress and has attracted commercial attention as a protecting agent. its production and application are restricted by the drawbacks of the discontinuous harvesting procedure involving salt shocks, which reduces volumetric yield, increases reactor corrosion, and complicates downstream processing. in order to synthesize ectoine continuously in less- ... | 2007 | 17369334 |
| chromohalobacter salarius sp. nov., a moderately halophilic bacterium isolated from a solar saltern in cabo de gata, almeria, southern spain. | a moderately halophilic, gram-negative bacterium (strain cg4.1(t)), which was isolated from a solar saltern at cabo de gata, a wildlife reserve located in the province of almería, southern spain, was subjected to a polyphasic taxonomic study. this organism was an aerobic, motile rod that produced colonies with a yellow pigment. strain cg4.1(t) grew at salinities of 3-25 % (w/v), at 15-45 degrees c and at ph 5-9. the organism reduced nitrate, hydrolysed starch and had phenylalanine deaminase acti ... | 2007 | 17551036 |
| modicisalibacter tunisiensis gen. nov., sp. nov., an aerobic, moderately halophilic bacterium isolated from an oilfield-water injection sample, and emended description of the family halomonadaceae franzmann et al. 1989 emend dobson and franzmann 1996 emend. ntougias et al. 2007. | an aerobic, moderately halophilic, gram-negative, motile, non-sporulating bacterium, strain lit2(t), was isolated from an oilfield-water injection after enrichment on crude oil. strain lit2(t) grew between 15 and 45 degrees c and optimally at 37 degrees c. it grew in the presence of 1-25 % (w/v) nacl, with an optimum at 10 % (w/v) nacl. predominant fatty acids were c(16 : 0) (26.9 %), c(18 : 1)omega7c (22.6 %), c(16 : 1)omega7c (20.4 %) c(19 : 0) cyclo omega8c (10.9 %) and c(17 : 0) (8 %). inter ... | 2007 | 17911302 |
| [phylogenetic diversity of culturable bacteria in the ancient salt deposits of the yipinglang salt mine, p. r. china]. | the microbial diversity of cultivable bacteria, isolated from the ancient salt deposits from the yipinglang salt mine (ypl) in the yunnan province, p. r. china,was investigated by using conventional culture-dependent method and phylogenetic analyses based on 16s rrna gene sequence comparisons. 38 bacteria strains were isolated from the brine, halite and saline soil samples on mba (marine broth agar 2216, difco) and isp 2 (international streotomyces project medium 2) media supplemented with 0.5-3 ... | 2007 | 17944352 |
| microbial diversity analysis of former salterns in southern taiwan by 16s rrna-based methods. | the microbiota diversity of the former salterns in southern taiwan was investigated by denaturing gradient gel electrophoresis (dgge) and fluorescence in situ hybridization (fish). soil samples from three salterns were analyzed using dgge and 16s rrna from 502 colonies representing 5 archaea and 18 bacteria taxonomic groups. each representative taxonomic group was further identified, whereas 8.7% of clones were unclassified microorganisms. chromohalobacter, halomonas and virgibacillus are domina ... | 2007 | 18072239 |
| molecular cloning and functional characterization of the genes encoding benzoate and p-hydroxybenzoate degradation by the halophilic chromohalobacter sp. strain hs-2. | chromohalobacter sp. strain hs-2 was isolated from salted fermented clams and analyzed for the ability to grow on benzoate and p-hydroxybenzoate as the sole carbon and energy source. hs-2 was characterized as moderately halophilic, with an optimal nacl concentration of 10%. the genes encoding the benzoate metabolism were cloned into a cosmid vector, sequenced, and then analyzed to reveal the benzoate (benabcd) and catechol (catbca) catabolic genes, both of which are flanked on either side by lys ... | 2008 | 18248426 |
| estimating p-coverage of biosynthetic pathways in dna libraries and screening by genetic selection: biotin biosynthesis in the marine microorganism chromohalobacter. | traditional approaches to natural product discovery involve cell-based screening of natural product extracts followed by compound isolation and characterization. their importance notwithstanding, continued mining leads to depletion of natural resources and the reisolation of previously identified metabolites. metagenomic strategies aimed at localizing the biosynthetic cluster genes and expressing them in surrogate hosts offers one possible alternative. a fundamental question that naturally arise ... | 2008 | 18493659 |
| unravelling the adaptation responses to osmotic and temperature stress in chromohalobacter salexigens, a bacterium with broad salinity tolerance. | chromohalobacter salexigens, a gammaproteobacterium belonging to the family halomonadaceae, shows a broad salinity range for growth. osmoprotection is achieved by the accumulation of compatible solutes either by transport (betaine, choline) or synthesis (mainly ectoine and hydroxyectoine). ectoines can play additional roles as nutrients and, in the case of hydroxyectoine, in thermotolerance. a supplementary solute, trehalose, not present in cells grown at 37 degrees c, is accumulated at higher t ... | 2008 | 18793408 |
| complete sequence of plasmid pmp1 from the marine environmental vibrio vulnificus and location of its replication origin. | a novel cryptic plasmid, pmp1, from an environmental vibrio vulnificus mp-4 isolated from mai po nature reserve in hong kong, has been characterized. the 7.6-kb plasmid had guanine-cytosine content of 40.03% and encoded four open reading frames (orfs) with >100 amino acids. the predicted protein of orf1 contained 478 amino acids showing 29% identity and 50% similarity over 309 amino acids to the integrase of vibrio cholerae phage vp2. orf2 encoded a putative protein of 596 amino acids, which wer ... | 2009 | 19009320 |
| racemase activity effected by two dehydrogenases in sulfolactate degradation by chromohalobacter salexigens: purification of (s)-sulfolactate dehydrogenase. | chromohalobacter salexigens dsm 3043, whose genome has been sequenced, is known to degrade (r,s)-sulfolactate as a sole carbon and energy source for growth. utilization of the compound(s) was shown to be quantitative, and an eight-gene cluster (csal_1764-csal_1771) was hypothesized to encode the enzymes in the degradative pathway. it comprised a transcriptional regulator (suyr), a tripartite tricarboxylate transporter-family uptake system for sulfolactate (slchfg), two sulfolactate dehydrogenase ... | 2010 | 20007648 |
| isethionate formation from taurine in chromohalobacter salexigens: purification of sulfoacetaldehyde reductase. | bacterial generation of isethionate (2-hydroxyethanesulfonate) from taurine (2-aminoethanesulfonate) by anaerobic gut bacteria was established in 1980. that phenomenon in pure culture was recognized as a pathway of assimilation of taurine-nitrogen. based on the latter work, we predicted from genome-sequence data that the marine gammaproteobacterium chromohalobacter salexigens dsm 3043 would exhibit this trait. quantitative conversion of taurine to isethionate, identified by mass spectrometry, wa ... | 2010 | 20133363 |
| amorphous ca-phosphate precursors for ca-carbonate biominerals mediated by chromohalobacter marismortui. | although diverse microbial metabolisms are known to induce the precipitation of carbonate minerals, the mechanisms involved in the bacterial mediation, in particular nucleation, are still debated. the study of aragonite precipitation by chromohalobacter marismortui during the early stages (3-7 days) of culture experiments, and its relation to bacterial metabolic pathways, shows that: (1) carbonate nucleation occurs after precipitation of an amorphous ca phosphate precursor phase on bacterial cel ... | 2010 | 20182524 |
| interplay between iron homeostasis and the osmotic stress response in the halophilic bacterium chromohalobacter salexigens. | in this study, the connection between iron homeostasis and the osmostress response in the halophile chromohalobacter salexigens was investigated. a decrease in the requirement for both iron and histidine and a lower level of siderophore synthesis were observed at high salinity, and these findings were correlated with a lower protein content in salt-stressed cells. a six-gene operon (cfuabc-fur-hisi-orf6 operon) located downstream of the ectabc ectoine synthesis genes was characterized. a fur str ... | 2010 | 20363778 |
| process optimization of the integrated synthesis and secretion of ectoine and hydroxyectoine under hyper/hypo-osmotic stress. | the synthesis and secretion of the industrial relevant compatible solutes ectoine and hydroxyectoine using the halophile bacterium chromohalobacter salexigens were studied and optimized. for this purpose, a cascade of two continuously operated bioreactors was used. in the first bioreactor, cells were grown under constant hyperosmotic conditions and thermal stress driving the cells to accumulate large amounts of ectoines. to enhance the overall productivity, high cell densities up to 61 g l(-1) w ... | 2010 | 20506292 |
| involvement of eupr, a response regulator of the narl/fixj family, in the control of the uptake of the compatible solutes ectoines by the halophilic bacterium chromohalobacter salexigens. | osmosensing and associated signal transduction pathways have not yet been described in obligately halophilic bacteria. chromohalobacter salexigens is a halophilic bacterium with a broad range of salt tolerance. in response to osmotic stress, it synthesizes and accumulates large amounts of the compatible solutes ectoine and hydroxyectoine. in a previous work, we showed that ectoines can be also accumulated upon transport from the external medium, and that they can be used as carbon sources at opt ... | 2010 | 20942908 |
| genome-scale reconstruction of metabolic network for a halophilic extremophile, chromohalobacter salexigens dsm 3043. | chromohalobacter salexigens (formerly halomonas elongata dsm 3043) is a halophilic extremophile with a very broad salinity range and is used as a model organism to elucidate prokaryotic osmoadaptation due to its strong euryhaline phenotype. | 2011 | 21251315 |
| chromohalobacter japonicus sp. nov., a moderately halophilic bacterium isolated from a japanese salty food. | a gram-negative, non-spore-forming, rod-shaped, motile bacterium, designated strain 43(t), was isolated from a japanese salty food and then subjected to a polyphasic taxonomic study. strain 43(t) is moderately halophilic, growing at nacl concentrations in the range 5-25 % (w/v), with optimum growth between 7.5 and 12.5 % (w/v) nacl. growth occurs at temperatures from 15 to 42 degrees c (optimally at 28-37 degrees c) and at ph 5.5-9.0 (optimally at ph 7.0-8.0). a phylogenetic analysis based on 16 ... | 2007 | 17911293 |
| reclassification of pseudomonas beijerinckii hof 1935 as chromohalobacter beijerinckii comb. nov., and emended description of the species. | pseudomonas beijerinckii (type strain dsm 7218(t)=atcc 19372(t)=ncimb 9041(t)) was isolated from salted beans and was first described by hof in 1935. 16s rrna gene sequence comparisons demonstrated its close relatedness (>97-99 %) to species of the genus chromohalobacter. a recent isolate from salted herrings originating from the baltic sea, strain 3b, also clustered phylogenetically within this genus. phenotypic features, substrate utilization, fatty acid profile, quinone and polar lipid compos ... | 2006 | 16902036 |
| the ectd gene, which is involved in the synthesis of the compatible solute hydroxyectoine, is essential for thermoprotection of the halophilic bacterium chromohalobacter salexigens. | the halophilic bacterium chromohalobacter salexigens synthesizes and accumulates compatible solutes in response to salt and temperature stress. (13)c-nuclear magnetic resonance analysis of cells grown in minimal medium at the limiting temperature of 45 degrees c revealed the presence of hydroxyectoine, ectoine, glutamate, trehalose (not present in cells grown at 37 degrees c), and the ectoine precursor, ngamma-acetyldiaminobutyric acid. high-performance liquid chromatography analyses showed that ... | 2006 | 16707670 |
| chromohalobacter nigrandesensis sp. nov., a moderately halophilic, gram-negative bacterium isolated from lake tebenquiche on the atacama saltern, chile. | a total of 52 strains of moderately halophilic bacteria isolated from hypersaline sediment of lake tebenquiche on the atacama saltern, chile, were subjected to a taxonomic study. the morphological, physiological, biochemical and nutritional characteristics of the strains matched those described for the genus chromohalobacter. cells were gram-negative, non-spore-forming, rod-shaped and motile. a black pigmentation was produced. one strain, designated lts-4n(t), grew optimally at 32 degrees c. gro ... | 2006 | 16514043 |
| opposing effects of nacl on reversibility and thermal stability of halophilic beta-lactamase from a moderate halophile, chromohalobacter sp. 560. | beta-lactamase from a moderately halophilic organism is expected to show salt-dependent stability. here we examined the temperature-dependence of stability at different salt concentrations using circular dichroism (cd) and enzyme activity. nacl showed opposing effects on melting temperature and reversibility of the thermal melting. increasing nacl concentration greatly increased the melting temperature from, e.g., 41 degrees c in the absence of nacl to 61 degrees c in 3 m nacl. conversely, rever ... | 2006 | 16256261 |
| changes in fatty acid composition of chromohalobacter israelensis with varying salt concentrations. | the adaptation of fatty acid composition of chromohalobacter israelensis, a euryhalophilic bacterium, grown at different salt concentrations was studied. c. israelensis tolerated nacl up to concentrations of 20% (w/v) and showed optimal growth at 7% (w/v). major fatty acids of this bacterium were palmitic acid (16:0), stearic acid (18:0), palmetoleic acid (16:1cisdelta9), and cis-vaccenic acid (18:1delta11). the salt concentration strongly influenced the fatty acid composition. in the presence o ... | 2005 | 15717227 |
| chromohalobacter sarecensis sp. nov., a psychrotolerant moderate halophile isolated from the saline andean region of bolivia. | a moderately halophilic, aerobic, motile, gram-negative, rod-shaped bacterium (strain lv4(t)) was isolated from saline soil around the lake laguna verde in the bolivian andes. the organism is a heterotroph, able to utilize various carbohydrates as a carbon source. it showed tryptophan deaminase, oxidase and catalase activity, but was unable to produce indole or h(2)s; nitrate was not reduced. the g+c content of the genomic dna was 56.1 mol%. the ph range for growth was 5-10, temperature range wa ... | 2004 | 15545411 |
| complex regulation of the synthesis of the compatible solute ectoine in the halophilic bacterium chromohalobacter salexigens dsm 3043t. | the synthesis of the compatible solute ectoine, mediated by the ectabc gene products, is the main mechanism used by the halophilic bacterium chromohalobacter salexigens to cope with osmotic stress. evidence was found that this process is regulated at the transcriptional level. s1 protection analyses performed with rna extracted from cells grown in minimal medium at low (0.75 m nacl) or high (2.5 m nacl) osmolarity suggested the existence of four promoters upstream of ecta. two of these (pecta1 a ... | 2004 | 15347763 |
| salt-inducible multidrug efflux pump protein in the moderately halophilic bacterium chromohalobacter sp. | it has been known that halophilic bacteria often show natural resistance to antibiotics, dyes, and toxic metal ions, but the mechanism and regulation of this resistance have remained unexplained. we have addressed this question by identifying the gene responsible for multidrug resistance. a spontaneous ofloxacin-resistant mutant derived from the moderately halophilic bacterium chromohalobacter sp. strain 160 showed a two- to fourfold increased resistance to structurally diverse compounds, such a ... | 2004 | 15294769 |
| gene transfer and expression of recombinant proteins in moderately halophilic bacteria. | moderately halophilic bacteria (mhb) of the genera halomonas and chromohalobacter have been used as hosts for the expression of heterologous proteins of biotechnological interest, thus expanding their potential to be used as cell factories for various applications. this chapter deals with the methodology for the construction of recombinant plasmids, their transfer to a number of mhb, and the assaying of the corresponding heterologous proteins activity. the transferred genes include (1) inaz, enc ... | 2004 | 15269426 |
| An extended suite of genetic tools for use in bacteria of the halomonadaceae: an overview. | Halophilic gammaproteobacteria of the family Halomonadaceae (including the genera Aidingimonas, Carnimonas, Chromohalobacter, Cobetia, Halomonas, Halotalea, Kushneria, Modicisalibacter, Salinicola, and Zymobacter) have current and promising applications in biotechnology mainly as a source of compatible solutes (powerful stabilizers of biomolecules and cells, with exciting potentialities in biomedicine), salt-tolerant enzymes, biosurfactants, and extracellular polysaccharides, among other product ... | 2012 | 22160899 |
| identification of moderately halophilic bacteria from thai fermented fish ( pla-ra ) and proposal of virgibacillus siamensis sp. nov. | forty-one isolates of moderately halophilic bacteria were isolated from fermented fish (pla-ra) in thailand. on the basis of their phenotypic and chemotaxonomic characteristics, dna-dna relatedness and 16s rrna gene sequences analyses, they were divided into six groups. the isolates in group i to v were gram-positive rod-shaped bacteria. they contained meso-diaminopimelic acid in the cell-wall peptidoglycan and menaquinone with seven isoprene units (mk-7). an isolate in group vi was a gram-negat ... | 2010 | 21099133 |
| taxonomic study of the genus salinicola: transfer of halomonas salaria and chromohalobacter salarius to the genus salinicola as salinicola salarius comb. nov. and salinicola halophilus nom. nov., respectively. | we have carried out a polyphasic taxonomic characterization of the type strains of the species with the recently validated name salinicola socius, together with two species that were phylogenetically closely related, halomonas salaria and chromohalobacter salarius. 16s rrna gene sequence analyses showed that they constituted a coherent cluster, with sequence similarities between 98.7 and 97.7 %. we have determined the almost complete 23s rrna gene sequences of these three type strains, and the p ... | 2010 | 19661506 |
| phylogenetic relationships within the family halomonadaceae based on comparative 23s and 16s rrna gene sequence analysis. | a phylogenetic study of the family halomonadaceae was carried out based on complete 16s rrna and 23s rrna gene sequences. several 16s rrna genes of type strains were resequenced, and 28 new sequences of the 23s rrna gene were obtained. currently, the family includes nine genera (carnimonas, chromohalobacter, cobetia, halomonas, halotalea, kushneria, modicisalibacter, salinicola and zymobacter). these genera are phylogenetically coherent except halomonas, which is polyphyletic. this genus compris ... | 2010 | 19656941 |
| aidingimonas halophila gen. nov., sp. nov., a moderately halophilic bacterium isolated from a salt lake. | two gram-negative, facultatively anaerobic, catalase-positive, oxidase-negative, non-motile, rod-shaped and moderately halophilic bacterial strains, designated yim 90637(t) and bh 017, were isolated from a salt lake in xinjiang province, north-west china, and subjected to a polyphasic taxonomic study. phylogenetic analysis based on 16s rrna gene sequences revealed that the two novel isolates were affiliated with the family halomonadaceae; the two strains shared 100 % sequence similarity, but sho ... | 2009 | 19643876 |
| [resistance of the petroleum-oxidizing microorganism dietzia sp. to hyperosmotic shock in reconstituted biofilms]. | a number of halotolerant and halophilic bacterial strains were isolated from the romashkinskoe oil field (tatarstan) stratal waters having a salinity of up to 100 g/l. the isolation of pure cultures involved biofilm reconstitution on m9 medium with paraffins. the associations obtained were dispersed and reinoculated onto solid media that contained either peptone and yeast extract (py) or paraffins. it was shown that such associations included both oil-oxidizing bacteria and accompanying chemohet ... | 2008 | 19004337 |
| high-pressure tolerance in halobacterium salinarum nrc-1 and other non-piezophilic prokaryotes. | in this study, we examined the high-pressure survival of a range of prokaryotes not found in high-pressure environments to determine the effects of adaptations to osmotic and oxidative stresses on piezo-resistance. the pressure survivals of halobacterium salinarum nrc-1, deinococcus radiodurans r1, and chromohalobacter salexigens were compared to that of escherichia coli mg1655. c. salexigens, which uses the compatible solute ectoine as an osmolyte, was as piezo-sensitive as e. coli mg1655, sugg ... | 2012 | 22212652 |
| halophilic properties of metal binding protein characterized by high histidine content from chromohalobacter salexigens dsm3043. | periplasmic metal binding protein characterized by high histidine content was cloned from moderate halophile, chromohalobacter salexigens. the protein, termed histidine-rich metal binding protein (hp), was expressed in and purified from e. coli as a native form. hp bound to ni- and cu-loaded chelate columns with high affinity, and co- and zn-columns with moderate affinity. although the secondary structure was not grossly altered by the addition of 0.2-2.0 m nacl, the thermal transition pattern w ... | 2012 | 22227860 |
| crystal structure of glucuronic acid dehydrogeanse from chromohalobacter salexigens. | | 2012 | 21987458 |
| cloning, expression, purification, crystallization and x-ray crystallographic analysis of glucuronic acid dehydrogenase from chromohalobacter salexigens. | glucuronic acid dehydrogenase (gluuadh), the product of the csal-2474 gene from the halophilic bacterium chromohalobacter salexigens dsm 3043, is an enzyme with potential use in the conversion of glucuronic acid in seaweed biomass to fuels and chemicals. gluuadh is an enzyme that catalyzes the oxidation of glucuronic acid (gluua) and galacturonic acid (galua) and has a preference for nad(+) rather than nadp(+) as a cofactor. recombinant gluuadh was crystallized in the presence of 0.2 m calcium a ... | 2011 | 21636913 |
| biodegradation of polyether algal toxins--isolation of potential marine bacteria. | marine algal toxins such as brevetoxins, okadaic acid, yessotoxin, and ciguatoxin are polyether compounds. the fate of polyether toxins in the aqueous phase, particularly bacterial biotransformation of the toxins, is poorly understood. an inexpensive and easily available polyether structural analog salinomycin was used for enrichment and isolation of potential polyether toxin degrading aquatic marine bacteria from florida bay area, and from red tide endemic sites in the south florida gulf coast. ... | 2010 | 20954040 |