| nucleotide sequences of the 3' terminal region of onion yellow dwarf virus isolates from allium plants in japan. | the 2032 nucleotide sequence of the 3' terminal region of onion yellow dwarf virus (oydv) isolated from allium wakegi, bearing the genes for viral coat protein (cp) and a truncated rna-dependent rna polymerase, has been determined. respective homologies of the nucleotide sequence in the corresponding region and the deduced amino acid sequence of cp with the equivalents of leek yellow stripe virus (lysv) from garlic were 68.0 and 59.3%. variation in the nucleotide sequence is concentrated in the ... | 1997 | 9354273 |
| nucleotide sequence analysis of virus isolates indicates the presence of three potyvirus species in allium plants. | cdnas of potyviruses from allium plants cultivated in different parts of the world were cloned by rt-pcr with a common primer for amplifying the 3' terminal genomic rnas of onion yellow dwarf virus (oydv), leek yellow stripe virus (lysv) and, probably, of closely related potyviruses. their nucleotide sequences bearing the viral coat protein (cp) gene and the 3' non-coding sequence were determined and compared. the degree of their sequence similarities clearly differentiated the respective viruse ... | 1998 | 9505969 |
| coat protein gene sequences of garlic and onion isolates of the onion yellow dwarf potyvirus (oydv). | partial genomic sequences from an unknown garlic potyvirus and from an onion isolate of the onion yellow dwarf potyvirus (oydv) were obtained. comparison of the deduced amino acid sequences showed a similarity of 88% between the respective viral coat proteins. the garlic potyvirus coat protein was expressed in e. coli cells, purified, and subjected to western blot analysis using antibodies raised against different garlic-infecting viruses. the expression protein was consistently recognised by an ... | 1996 | 9526536 |
| molecular characterisation of a complex mixture of viruses in garlic with mosaic symptoms in china. | degenerate primers were used to detect and amplify cdna of viruses of the genera carlavirus, allexivirus and potyvirus from garlic plants with mosaic symptoms growing in zhejiang province, china. plants contained a complex mixture of viruses and strains. three distinct stains of garlic latent virus were detected; the most frequent one was completely sequenced and partial sequences were obtained for the other two. the complete sequence (8363 nt) was 76.4% identical to a korean isolate. two allexi ... | 2001 | 11722009 |
| characterisation of some carla- and potyviruses from bulb crops in china. brief report. | conserved carla- and potyvirus primers were used in rt-pcr to amplify virus fragments from garlic and other bulb crops in china and the fragments were subsequently sequenced and compared in phylogenetic analyses. garlic plants from henan, hubei, jiangsu, shangdong and yunnan provinces all contained at least one isolate each of garlic latent virus (genus carlavirus), onion yellow dwarf virus (oydv, genus potyvirus) and leek yellow stripe virus (lysv, genus potyvirus). the complete sequence of a z ... | 2002 | 11890533 |
| occurrence and sequences of lily mottle virus and lily symptomless virus in plants grown from imported bulbs in zhejiang province, china. | degenerate primers were used to amplify virus sequences from imported lilies in zhejiang province, china. two viruses, lily mottle virus (lmov, genus potyvirus) and lily symptomless virus (lsv, genus carlavirus) were detected, purified and completely sequenced from a mixed infection in a plant raised from bulbs imported from the netherlands. the sequence of lmov was 9644 nt long and encoded a polyprotein of 3095 amino acids with a calculated m(r) of 351.0 kda that had only 45.1-54.4% identity to ... | 2003 | 14648295 |
| complete nucleotide sequences of attenuated and severe isolates of leek yellow stripe virus from garlic in northern japan: identification of three distinct virus types in garlic and leek world-wide. | we have determined the complete nucleotide sequence of three isolates (two severe and one attenuated) of the potyvirus leek yellow stripe virus (lysv), the main causal agent of the garlic mosaic disease that is prevalent in aomori prefecture, northern japan. they contained 10,296-10,297 nucleotides and encoded a deduced polyprotein of 3,215 amino acids. sequence variation among the three isolates was 1.5% at both the nucleotide and amino acid levels. surprisingly, the sequences of all three isol ... | 2005 | 15703850 |
| a new potyvirus from thunberg fritillary (fritillaria thunbergii miq.) in zhejiang, china. | a potyvirus causing mosaic symptoms in thunberg fritillary (fritillaria thunbergii) was found at two sites in zhejiang province, china. the virus was readily mechanically transmitted to its original host but not to any of 17 other widely used plant virus indicators. a polyclonal antiserum raised to purified virus particles reacted with its homologous virus but not with a range of other viruses (including 16 potyvirus species). in electron microscopy, virus particles and inclusion bodies typical ... | 2005 | 15789262 |
| molecular characterization of czech and chinese leek yellow stripe virus isolates from garlic. | using an rt-pcr specific for nuclear inclusion b (nib) and coat protein (cp) genes of leek yellow stripe virus (lysv) we detected two czech (lysv-5cz and lysv-22cz) and one chinese (lysv-16) isolate of lysv in garlic plants. the rt-pcr products were cloned and sequenced. phylogenetic analysis based on deduced amino acid sequence of nib-cp region placed the czech isolates in the group i and the chinese isolate in the group ii of lysv. | 2006 | 17131941 |
| identification and sequence analysis of potyviruses infecting crops in vietnam. | fifty-two virus isolates from 13 distinct potyvirus species infecting crops in vietnam were identified and the 3' region of each genome was sequenced. the viruses were: bean common mosaic virus (bcmv), potato virus y (pvy), sugarcane mosaic virus (scmv), sorghum mosaic virus (srmv), chilli veinal mottle virus (chivmv), zucchini yellow mosaic virus (zymv), leek yellow stripe virus (lymv), shallot yellow stripe virus (sysv), onion yellow dwarf virus (oydv), turnip mosaic virus (tumv), dasheen mosa ... | 2008 | 17906829 |
| further evidence that shallot yellow stripe virus (sysv) is a distinct potyvirus and reidentification of welsh onion yellow stripe virus as a sysv strain. | abstract an antiserum to shallot yellow stripe virus (sysv) was raised and used in combination with a range of other antisera to potyviruses of allium spp. in electron microscopic decoration experiments. the serological results corroborated an earlier finding that the type isolates of sysv and welsh onion yellow stripe virus (woysv) are closely related to each other and only distantly related to onion yellow dwarf (oydv) and leek yellow stripe (lysv) viruses, the two other major potyviruses infe ... | 1999 | 18944789 |
| development of a short oligonucleotide microarray for the detection and identification of multiple potyviruses. | the genus potyvirus is the largest and one of the most economically important virus genera infecting plants. however, current diagnostic techniques are limited in their ability to identify multiple potyvirus infections. an assay that can identify multiple potyviruses simultaneously, with good specificity and sensitivity, is therefore highly desirable. to determine the feasibility of simultaneous detection of multiple potyviruses a 25-mer oligonucleotide microarray was developed targeting four di ... | 2009 | 19654025 |
| the global trade in fresh produce and the vagility of plant viruses: a case study in garlic. | as cuisine becomes globalized, large volumes of fresh produce are traded internationally. the potential exists for pathogens infecting fresh produce to hitchhike to new locations and perhaps to establish there. it is difficult to identify them using traditional methods if pathogens are novel, scarce, and/or unexpected. in an attempt to overcome this limitation, we used high-throughput sequencing technology as a means of detecting all rna viruses infecting garlic (allium sativum l.) bulbs importe ... | 2014 | 25133543 |
| viruses occurring in onion crop in amasya province, the major onion producing region in turkey. | amasya is the greatest onion producing area in turkey. onion fields from amasya region were surveyed for virus diseases in 2009-2011 and tested for the presence of the most important onion viruses such as onion yellow dwarf virus (oydv), iris yellow spot virus (iysv), leek yellow stripe virus (lysv), shallot latent virus (slv) and garlic common latent virus (gclv). the presence of virus diseases and their identification was ascertained through symptom observation in the fields, sap transmission ... | 2013 | 24426263 |
| use of tissue culture techniques for producing virus-free plant in garlic and their identification through real-time pcr. | this study was performed for comparison of meristem culture technique with shoot tip culture technique for obtaining virus-free plant, comparison of micropropagation success of two different nutrient media, and determination of effectiveness of real-time pcr assay for the detection of viruses. two different garlic species (allium sativum and allium tuncelianum) and two different nutrient media were used in this experiment. results showed that medium 2 was more successful compared to medium 1 for ... | 2013 | 23935432 |
| variability in the p1 gene helps to refine phylogenetic relationships among leek yellow stripe virus isolates from garlic. | nucleotide sequences from the p1 gene and the 5' untranslated region of leek yellow stripe virus (lysv), collected from several locations, were used to refine the phylogenetic relationships among the isolates. multiple alignments revealed three distinct regions of insertions and deletions to classify lysvs. in our phylogenetic analyses, the lysv isolates separated into two major groups (n-type and s-type). s-type viruses had two large deletions compared to n-type viruses. considering that the ou ... | 2012 | 21964945 |
| ultra-sensitive detection of two garlic potyviruses using a real-time fluorescent (taqman) rt-pcr assay. | a method for the detection of onion yellow dwarf virus (oydv) and leek yellow stripe virus (lysv), the two most prevalent garlic potyviruses, has been developed that combines ic-rt-pcr/rt-pcr with the use of taqman probes. comparisons with elisa results obtained with identical oydv and lysv infected samples showed sensitivity in detecting these viruses increased up to 10(6)-fold. oydv and lysv were detected using different fluorochromes in the probe, thus allowing unequivocal diagnosis for each ... | 2004 | 15158064 |