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a30641, a new epidithiodiketopiperazine with antifungal activity.a new antibiotic, designated a30641, having in vitro activity against gram-positive bacteria and fungi has been isolated from a strain of aspergillus tamarii. chemical and physical characterization indicate that it is a member of the class of antibiotics containing the epidithiodiketopiperazine moiety.1976931808
[invasive nasosinusal aspergillosis in an immunocompetent patient].the authors describe a case of invasive nasosinusal aspergillosis in an immunocompetent patient. after a careful bibliographic review, they emphasize that in the present case the diagnosis was made by means of fungal culture as the clinical picture, imaging techniques and histopathological findings were aspecific. the pathogenetic agent resulted aspergillus tamarii which belongs to the aspergillus flavus group and is one of the most unusual agents in literature. the treatment of choice, in the i ...19921307449
behaviour of aspergillus flavus in presence of aspergillus niger during biosynthesis of aflatoxin b.aspergillus niger or aspergillus tamarii when grown as mixed cultures with toxigenic a. flavus inhibits biosynthesis of aflatoxin by a. flavus, owing primarily to its ability to produce inhibitors of aflatoxin biosynthesis and to their ability to degrade aflatoxin. gluconic acid partly prevents aflatoxin production. the other factors such as changes in ph of the medium and the effect on the growth of a. flavus have no role in imparting capabilities to these cultures to inhibit aflatoxin producti ...19902124794
[screening of beta-n-acetylhexosaminidase forming strains and conditions for enzyme production].about 1200 strains of microorganisms were screened including fungi, actinomyces, and bacteria, in which 237 strains producing the enzyme desired. the results showed that the beta-glcnacase and beta-galnacase always co-existed in one strain, though may be in different ratio. from strains mentioned above the authors screened out a potent beta-n-acetylhexosaminidase producing strain, aspergillus tamarii s215, from the soil sample. the optimal conditions for enzyme production were as follows: the mi ...19902141744
[purification and characterization of beta-n-acetyl hexosaminidase from aspergillus tamarii].a beta-n-acetylhexosaminidase from mycelium-free culture filtrate of asp tamarii s215 was purified to page homogenous by ammonium sulfate and polyethylene glycol fractionation precipitation followed by sephadex g-50 desalt, deae-sephadex a-25 chromatography, hydroxyapatite chromatography and deae-sephadex a-25 rechromatography with 170-fold purification and 24.7% recovery. the ratio of the beta-glcnacase and beta-galnacase was 2.5 and remained constant throughout the purification. the mr estimat ...19902147528
aspergillus nomius, a new aflatoxin-producing species related to aspergillus flavus and aspergillus tamarii.aspergillus nomius is described and represents a new aflatoxigenic species phenotypically similar to a. flavus. strains examined were isolated from insects and agricultural commodities. separation from a. flavus is based on the presence of indeterminate sclerotia and a lower growth temperature. comparisons of dna relatedness show a. nomius to have only relatively recently evolved from a. flavus and a. tamarii.19873116923
comparison of airborne fungi in evaporative cooled and air conditioned homes.the evaporative cooler (ec) is commonly used during the warmer months in millions of homes in the desert regions of the southwestern united states. in this investigation fungi isolated from ec homes were compared with recoveries from air conditioned (ac) homes. aspergillus tamarii was more abundant in ec homes (p less than .05) whereas penicillium spp. were more prevalent in ac homes (p less than .05). fusarium was recovered only from ec homes. overall, more fungi were isolated from ec homes tha ...19873662133
microbial hydroxylation of 5-anilino-1,2,3,4-thiatriazole.two hundred eighty-five fungi, including 100 basidiomycetes and 35 yeasts, 75 actinomycetes, and 40 bacteria were screened for their ability to convert 5-anilino-1,2,3,4-thiatriazole (at) to 5-(p-hydroxyanilino)-1,2,3,4-thiatriazole (p-ht). eleven cultures were found that formed p-ht, which was isolated and whose structure was determined. aspergillus tamarii nrrl 3280 formed 8.6 g of p-ht/liter from 10 g of at/liter (78.9% conversion) in shaken flasks and 4.57 g of p-ht/liter from 6 g of at/lite ...19734699219
[study of processes of respiraion and amino acid synthesis in variants of aspergillus tamarii obtained by the action of ethyleneimine]. 19675601499
microbial transformation of a series of androgens with aspergillus tamarii. 19676041431
glycosidases induced in aspergillus tamarii. mycelial alpha-d-galactosidases.two alpha-d-galactosidases (alpha-d-galactoside galactohydrolase, ec 3.2.1.22) produced by aspergillus tamarii were purified from the mycelial extract by a procedure including chromatography on hydroxyapatite, deae-cellulose and ecteola-cellulose. each of these enzymes showed a single protein band corresponding to the alpha-d-galactosidase activity when examined by polyacrylamide-gel electrophoresis. they catalysed the hydrolysis of o-nitrophenyl alpha-d-galactoside, melibiose, raffinose and sta ...19846331398
glycosidases induced in aspergillus tamarii. secreted alpha-d-galactosidase and beta-d-mannanase.an alpha-d-galactosidase (ec 3.2.1.22) and a beta-d-mannanase (ec 3.2.1.78), which were secreted into the growth medium when aspergillus tamarii was cultivated in the presence of galactomannan, were purified by a procedure including chromatography on hydroxyapatite and deae-cellulose columns. each of these enzymes showed a single protein band, corresponding to their respective activities, on polyacrylamide-gel electrophoresis. both enzymes were shown to be glycoproteins containing n-acetylglucos ...19846331399
production of cyclopiazonic acid by aspergillus tamarii kita.production of the mycotoxin cyclopiazonic acid by aspergillus tamarii kita is reported for the first time. examination of 23 isolates of the fungus showed that 22 produced the toxin under the culture conditions utilized.19836660879
production of fumigaclavine a by aspergillus tamarii kita.aspergillus tamarii kita. isolated from seeds of paspalum scrobiculatum l. is found to produce ergot alkaloids in submerged culture. the culture filtrate and mycelium are observed to contain 0.125 mg/ml and 1.2 mg/g (dry weight) total alkaloids consisting of 86.5 and 91.3% fumigaclavine a, respectively. the identification of the compound was confirmed by high-performance liquid chromatography, ultraviolet, infrared, and mass spectrophotometry analyses. this is the first report of the production ...19846713307
sequence variability in homologs of the aflatoxin pathway gene aflr distinguishes species in aspergillus section flavi.the aspergillus parasiticus aflr gene, a gene that may be involved in the regulation of aflatoxin biosynthesis, encodes a putative zinc finger dna-binding protein. pcr and sequencing were used to examine the presence of aflr homologs in other members of aspergillus section flavi. the predicted amino acid sequences indicated that the same zinc finger domain, ctscasskvrctkekpacarcierglac, was present in all of the aspergillus sojae, aspergillus flavus, and aspergillus parasiticus isolates examined ...19957887625
aflatoxin and cyclopiazonic acid production by a sclerotium-producing aspergillus tamarii strain.the production of aflatoxins b1 and b2 by aspergillus tamarii (subgenus circumdati section flavi) is reported for the first time. the fungus was isolated from soil collected from a tea (camellia sinensis) field in miyazaki prefecture, japan. three single-spore cultures, nrrl 25517, nrrl 25518, and nrrl 25519, were derived from subcultures of the original isolate 19 (mz2). each of these single-spore cultures of a. tamarii produced aflatoxins b1 and b2 and cyclopiazonic acid, as well as black, pea ...19968899995
detection of aspergillus ribosomal rna using biotinylated oligonucleotide probes.aspergillosis continues to be a devastating disease entity that results in significant mortality in immunosuppressed patients. rapid diagnosis is often required to initiate appropriate therapy. although the histopathologist may be able to visualize fungal organisms in tissue specimens, the histology of aspergillus species may overlap with a variety of fungi, so diagnosis often relies on fungal cultures that can take weeks to complete. recently, an in situ hybridization assay targeting aspergillu ...19979458383
properties of aspergillus tamarii, a. caelatus and related species from acidic tea field soils in japan.fungi in aspergillus section flavi include both aflatoxin producers and non-producers. aspergillus caelatus is a recently described non-aflatoxigenic species in this section, which has some common characteristics with a. tamarii, such as yellowish brown color and double walled spores. in contrast to the morphological similarities, all of the a. caelatus isolates tested produced no cyclopiazonic acid whereas most isolates of a. tamarii produce this compound. there are six nucleotide differences t ...199810531683
molecular and physiological aspects of aflatoxin and sterigmatocystin biosynthesis by aspergillus tamarii and a. ochraceoroseus.until recently, only three species (aspergillus flavus, a. parasiticus and a. nomius) have been widely recognized as producers of aflatoxin. in this study we examine aflatoxin production by two other species, a. tamarii and a. ochraceoroseus, the latter of which also produces sterigmatocystin. toxin-producing strains of a. tamarii and a. ochraceoroseus were examined morphologically, and toxin production was assayed on different media at different ph levels using thin layer chromatography and a d ...200010855723
catalytic properties of the immobilized aspergillus tamarii xylanase.xylanase from aspergillus tamarii was covalently immobilized on duolite a147 pretreated with the bifunctional agent glutaraldehyde. the bound enzyme retained 54.2% of the original specific activity exhibited by the free enzyme (120 u/mg protein). compared to the free enzyme, the immobilized enzyme exhibited lower optimum ph, higher optimum reaction temperature, lower energy of activation, higher km (michaelis constant), lower vmax (maximal reaction rate). the half-life for the free enzyme was 18 ...200212501991
potential risk of acute hepatotoxicity of kodo poisoning due to exposure to cyclopiazonic acid.kodo millet (paspalum scrobiculatum l.) is a staple food of some sections of people of north india. consumption of kodo millet is often found to cause intoxication and poisoning. the grains are frequently infested with aspergillus tamarii kita, which produced substantial amount of a mycotoxin, cyclopiazonic acid (cpa). investigations were carried out to evaluate the hepatotoxic/preneoplastic changes in rat liver following single and multiple dose administration of cpa. results showed a marked in ...200312860310
transformation of progesterone and related steroids by aspergillus tamarii. 196514285669
effect of the nitrogen source on caffeine degradation by aspergillus tamarii.to evaluate caffeine degradation and nitrogen requirements during aspergillus tamarii growth in submerged culture.200414687215
flexibility of the endogenous progesterone lactonisation pathway in aspergillus tamarii kita: transformation of a series of cortical steroid analogues.a range of cortical steroids have been transformed by the fungus aspergillus tamarii, which has the ability to convert progesterone to testololactone in high yield through a four step enzymatic pathway. 16alpha,17alpha-epoxyprogesterone underwent a rare epoxide opening resulting in a unique inversion of stereochemistry to give 16beta-hydroxy-17alpha-oxa-d-homo-androst-4-en-3,17-dione. the metabolism of deoxycorticosterone resulted in relatively efficient transformation to testololactone with no ...200314698211
a thermostable maltose-tolerant alpha-amylase from aspergillus tamarii.an homogeneous fraction of alpha-amylase from aspergillus tamarii was obtained by means of a very easy purification procedure. the enzyme is a glycoprotein containing 32% saccharide and mw of 37.5 kda. optimal of ph and temperature with starch as substrate were 4.5-6.5 and 50-55 degrees c. the enzyme was stable for several hours at temperature up to 65 degrees c. starch, amylose, and amylopectin were the substrates preferentially hydrolysed and maltose and maltotriose were the main end products. ...200414768025
production of l-asparaginase by filamentous fungi.l-asparaginase production was investigated in the filamentous fungi aspergillus tamarii and aspergillus terreus. the fungi were cultivated in medium containing different nitrogen sources. a. terreus showed the highest l-asparaginase (activity) production level (58 u/l) when cultivated in a 2% proline medium. both fungi presented the lowest level of l-asparaginase production in the presence of glutamine and urea as nitrogen sources. these results suggest that l-asparaginase production by of filam ...200415543411
fate of novel quasi reverse steroidal substrates by aspergillus tamarii kita: bypass of lactonisation and an exclusive role for the minor hydroxylation pathway.the fungus aspergillus tamarii transforms progesterone to testololactone in high yield through a flexible four-step enzymatic pathway. to date no studies have investigated the effect of transposition of steroidal functionality between ring-a and ring-d in order to determine the effect on steroidal metabolism. a series of novel quasi reverse steroids (7-9) were synthesised through linz and schafer oxidation where 14-en-16-one functionality is generated on ring-d of the steroid. to retain parity w ...200515904875
tropical soil microflora of spice-based cropping systems as potential antagonists of root-knot nematodes.suppression of plant parasitic nematodes with nematode predators, parasites or antagonists is an eco-friendly approach than the toxic chemicals. in a study, soil borne fungi from the rhizosphere of major spice crops were collected from diverse cropping systems prevailing in three southern states of india. a series of in vitro studies were conducted using 73 freshly collected fungal isolates and 76 isolates obtained from other sources. out of this 67 isolates were not parasitic on females of root ...200515955340
onychomycosis due to aspergillus tamarii in a 3-year-old boy. 200516040417
utilization of methylthio-s-triazine for growth of soil fungi.aspergillus niger van tieghem, aspergillus tamarii kita, and aspergillus flavus link ex fries utilized the methylthio moiety of 2,4-bis(isopropylamino) -6-methyl-mercapto-s-triazine (prometryne) as a sulfur nutrient source. other soil fungal isolates not affected by prometryne concentrations to 1 mg/ml culture included: aspergillus oryzae (ahlburg) cohn, curvularia lunata (wakker) boedijn, trichoderma viride persoon ex fries, alternaria tenuis nees ex corda, penicillium funiculosum thom, and pae ...197016349873
ring-b functionalized androst-4-en-3-ones and ring-c substituted pregn-4-en-3-ones undergo differential transformation in aspergillus tamarii kita: ring-a transformation with all c-6 substituted steroids and ring-d transformation with c-11 substituents.the fungus aspergillus tamarii transforms progesterone 1 into testololactone 5 in high yield through a four-step enzymatic pathway which is flexible to a range of steroidal substrates. to date, no studies have investigated the fate of c-6 (ring-b) and c-11 (ring-c) functionalized steroidal substrates on metabolism. remarkably all of the c-6 functionalized substrates underwent reductive metabolism on ring-a in contrast to c-11 functionalized steroids where only ring-d oxidative or reductive trans ...200616574481
the metabolism of aspergillus tamarii kita. kojic acid production. 193816746688
isolation, characterization and optimization of culture parameters for production of an alkaline protease isolated from aspergillus tamarii.aspergillus tamarii expresses an extracellular alkaline protease that we show to be effective in removing hair from cattle hide. large quantities of the enzyme will be required for the optimization of the enzymatic dehairing process so the growth conditions for maximum protease expression by a. tamarii were optimized for both solid-state culture on wheat bran and for broth culture. optimal protease expression occurred, for both cultural media, at initial ph 9; the culture was incubated at 30 deg ...200717245587
distinct metabolic handling of 3beta-hydroxy-17a-oxa-d-homo-5alpha-androstan-17-one by the filamentous fungus aspergillus tamarii kita: evidence in support of steroid/hydroxylase binding hypothesis.aspergillus tamarii kita transforms progesterone in to testololactone in high yield through a sequential four-step enzymatic pathway which also has the flexibility to transform a range of steroidal substrates. this study has investigated the further metabolism of testololactone and a range of fully saturated steroidal lactone analogues. in contrast to testololactone, which even after 120 h incubation did not undergo further metabolism, the lactone analogues entered the minor hydroxylation pathwa ...200717692565
case of keratitis caused by aspergillus tamarii.we report a case of aspergillus tamarii keratitis. ocular injury was known to be a predisposing factor. topical natamycin and econazole treatment and subsequent systemic ketoconazole treatment proved effective. the isolate was identified by morphological characteristics and sequence analysis as a. tamarii, a member of aspergillus section flavi not hitherto reported from keratomycosis.200717699648
[rapid improvement of lipase production in penicillium expansum by genome shuffling].in the present study, the genome shuffling was used to improve lipase production of penicillium expansum. a lipase producing mutant strain-penicillium expansum fs8486 and a wild type of aspergillus tamarii fs-132 isolated from soil of a volcano in xinjiang were used as the parental strains. after two rounds of genome shuffling, several elite daughter strains were screened. the lipase activity in one of the daughter strains was increased 317% over the starting strain fs8486. comparisons of the mo ...200717822042
transformation of 5-ene steroids by the fungus aspergillus tamarii kita: mixed molecular fate in lactonization and hydroxylation pathways with identification of a putative 3beta-hydroxy-steroid dehydrogenase/delta5-delta4 isomerase pathway.the fungus aspergillus tamarii metabolizes progesterone to testololactone in high yield through a sequential four step enzymatic pathway which, has demonstrated flexibility in handling a range of steroidal probes. these substrates have revealed that subtle changes in the molecular structure of the steroid lead to significant changes in route of metabolism. it was therefore of interest to determine the metabolism of a range of 5-ene containing steroidal substrates. remarkably the primary route of ...200919136076
transformation of some 3alpha-substituted steroids by aspergillus tamarii kita reveals stereochemical restriction of steroid binding orientation in the minor hydroxylation pathway.aspergillus tamarii contains an endogenous lactonization pathway which can transform progesterone to testololactone in high yield through a sequential four step enzymatic pathway. in this pathway testosterone is formed which primarily undergoes oxidation of the c-17beta-alcohol to a c-17 ketone but, can also enter a minor hydroxylation pathway where 11beta-hydroxytestosterone is produced. it was recently demonstrated that this hydroxylase could monohydroxylate 3beta-hydroxy substituted saturated ...201020026270
molecular strategy for identification in aspergillus section flavi.abstract aspergillus flavus is one of the most common contaminants that produces aflatoxins in foodstuffs. it is also a human allergen and a pathogen of animals and plants. aspergillus flavus is included in the aspergillus section flavi that comprises 11 closely related species producing different profiles of secondary metabolites. a six-step strategy has been developed that allows identification of nine of the 11 species. first, three real-time pcr reactions allowed us to discriminate four grou ...201020113354
molecular strategy for identification in aspergillus section flavi.aspergillus flavus is one of the most common contaminants that produces aflatoxins in foodstuffs. it is also a human allergen and a pathogen of animals and plants. aspergillus flavus is included in the aspergillus section flavi that comprises 11 closely related species producing different profiles of secondary metabolites. a six-step strategy has been developed that allows identification of nine of the 11 species. first, three real-time pcr reactions allowed us to discriminate four groups within ...201020377644
in vitro susceptibility of aspergillus spp. to dithiocarbamate organoruthenium compounds.summary the in vitro antifungal activity of ruthenium dithiocarbamate compounds (1-5) was investigated and assessed for its activity against seven different species of aspergillus (aspergillus clavatus, aspergillus flavus, aspergillus fumigatus, aspergillus niger, aspergillus nomius, aspergillus tamarii and aspergillus terreus). analysis of in vitro susceptibility was performed using broth microdilution assay following the clinical and laboratory standards institute guidelines for filamentous fu ...201020557461
aspergillus section flavi and aflatoxins in algerian wheat and derived products.wheat and its derivatives are a very important staple food for north african populations. the aim of this study was to analyze populations of aspergillus section flavi from local wheat based on aflatoxins (afs), cyclopiazonic acid (cpa) and sclerotia production, and also to evaluate afs-contaminated wheat collected from two different climatic regions in algeria. a total of 108 samples of wheat were collected during the following phases: pre-harvest, storage in silos and after processing. the res ...201020627118
cloning and expression of aspergillus tamarii fs132 lipase gene in pichia pastoris.a lipase gene (atl) was cloned from aspergillus tamarii fs132 for the first time. the gene was found to have an open reading frame of 1024 base pairs (bp), and the coding region of the gene contained two introns (51 bp and 52 bp). multi-alignment analysis of the deduced amino acid sequence indicated high homology between the enzyme and mono-and diacylglycerol lipases from fungi aspergillus. the recombinant lipase was expressed in pichia pastoris gs115 cells. the recombinant lipase was found to h ...201020640158
physico-chemical and clinico-immunologic studies on the allergenic significance of aspergillus tamarii, a common airborne fungus.aspergillus-derived inhalant allergens play an important role in the etiology of allergic respiratory diseases. in the present study, we investigated the allergenic potential of aspergillus tamarii, quantified its airborne content, identified its major/minor allergens, evaluated heterogeneity of patients' ige response to its allergens and cross-reactivity of its allergens with other aspergillus allergens. skin prick tests with a tamarii extract were performed on 300 patients of bronchial asthma/ ...201120667623
transformation of a series of saturated isomeric steroidal diols by aspergillus tamarii kita reveals a precise stereochemical requirement for entrance into the lactonization pathway.four isomers of 5α-androstan-3,17-diol have been transformed by the filamentous fungus aspergillus tamarii, an organism which has the ability to convert progesterone to testololactone in high yield through an endogenous four step enzymatic pathway. the only diol handled within the lactonization pathway was 5α-androstan-3α,17β-diol which, uniquely underwent oxidation of the 17β-alcohol to the 17-ketone prior to its baeyer-villiger oxidation and the subsequent production of 3α-hydroxy-17a-oxa-d-ho ...201020832471
species identification of aspergillus section flavi isolates from portuguese almonds using phenotypic, including maldi-tof icms, and molecular approaches.aims: section flavi is one of the most significant sections in the genus aspergillus. taxonomy of this section currently depends on multivariate approaches, entailing phenotypic and molecular traits. this work aimed to identify isolates from section flavi by combining various classic phenotypic and genotypic methods as well as the novel approach based on spectral analysis by maldi-tof icms, and to evaluate the discriminatory power of the various approaches in species identification. methods and ...201121790915
Energetics of growth of Aspergillus tamarii in a biological real-time reaction calorimeter.Fungal cultivation in a biological real-time reaction calorimeter (BioRTCal) is arduous due to the heterogeneous nature of the system and difficulty in optimizing the process variables. The aim of this investigation is to monitor the growth of fungi Aspergillus tamarii MTCC 5152 in a calorimeter. Experiments carried out with a spore concentration of 10(5) spores/mL indicate that the growth based on biomass and heat generation profiles was comparable to those obtained hitherto. Heat yield due to ...201122113563
mycoremediation of endosulfan and its metabolites in aqueous medium and soil by botryosphaeria laricina jas6 and aspergillus tamarii jas9.microbial degradation offers an efficient and ecofriendly approach to remove toxicants from the contaminated environments. botryosphaeria laricina jas6 and aspergillus tamarii jas9 were capable of degrading endosulfan and their metabolites which were isolated through enrichment technique. both the strains were able to withstand an exposure of 1300 mg/l and showed luxuriant growth at 1000 mg/l of endosulfan. the change in ph in the culture broth was from 6.8 to 3.4 and 3.8 during growth kinetic s ...201324130848
lytic enzyme production optimization using low-cost substrates and its application in the clarification of xanthan gum culture broth.lytic enzymes are widely used in industrial biotechnology as they are able to hydrolyze the bacterial cell wall. one application of these enzymes is the clarification of the culture broth for the production of xanthan gum, because of its viability in viscous media and high specificity. the screening process for filamentous fungi producing lytic enzymes, the optimization of production of these enzymes by the selected microorganism, and the optimization of the application of the enzymes produced i ...201425473487
does fungicide application in vineyards induce resistance to medical azoles in aspergillus species?this study assessed if the use of sterol demethylase inhibitor fungicides in vineyard production can induce resistance to azoles in aspergillus strains and if it can induce selection of resistant species. we also tried to identify the aspergillus species most prevalent in the vineyards. two vineyards from northern portugal were selected from "vinhos verdes" and "douro" regions. the vineyards were divided into plots that were treated or not with penconazole (pen). in each vineyard, air, soil, and ...201424833021
distribution of aflatoxigenic aspergillus section flavi in commercial poultry feed in nigeria.the distribution and aflatoxigenicity of aspergillus section flavi isolates in 58 commercial poultry feed samples obtained from 17 states in five agro-ecological zones (aezs) in nigeria were determined in order to assess the safety of the feeds with respect to aflatoxin-producing fungi. correlation was also performed for incidence of species, aflatoxin-producing ability of isolates in vitro, and aflatoxin (afb1) concentrations in the feed. a total of 1006 aspergillus section flavi isolates were ...201425108761
population structure and aflatoxin production by aspergillus sect. flavi from maize in nigeria and ghana.aflatoxins are highly toxic carcinogens that contaminate crops worldwide. previous studies conducted in nigeria and ghana found high concentrations of aflatoxins in pre- and post-harvest maize. however, little information is available on the population structure of aspergillus sect. flavi in west africa. we determined the incidence of aspergillus sect. flavi and the level of aflatoxin contamination in 91 maize samples from farms and markets in nigeria and ghana. aspergillus spp. were recovered f ...201424750813
misidentification of aspergillus nomius and aspergillus tamarii as aspergillus flavus: characterization by internal transcribed spacer, β-tubulin, and calmodulin gene sequencing, metabolic fingerprinting, and matrix-assisted laser desorption ionization-time of flight mass spectrometry.aspergillus nomius and aspergillus tamarii are aspergillus species that phenotypically resemble aspergillus flavus. in the last decade, a number of case reports have identified a. nomius and a. tamarii as causes of human infections. in this study, using an internal transcribed spacer, β-tubulin, and calmodulin gene sequencing, only 8 of 11 clinical isolates reported as a. flavus in our clinical microbiology laboratory by phenotypic methods were identified as a. flavus. the other three isolates w ...201424452174
primary cutaneous aspergillosis due to aspergillus tamarii in an immunocompetent host.primary cutaneous aspergillosis is a rare disease usually caused by aspergillus fumigatus, aspergillus flavus, aspergillus terreus and aspergillus ustus. it is usually seen in immunocompromised hosts, though some cases are also reported in immunocompetent hosts. we present a case of an immunocompetent farmer who presented with generalised nodules and plaques, mimicking erythema nodosum leprosum but turned out to be cutaneous aspergillosis caused by aspergillus tamarii. the characteristic ascospo ...201323970496
fungal spore concentrations in indoor and outdoor air in university libraries, and their variations in response to changes in meteorological variables.the fungal spore concentration (fsc) in the air poses a risk for human health. this work studied the fsc in university libraries and how it is affected by environmental factors. a total of 347 samples were obtained using a microbio mb2(®) aerosol sampler. the wind speed (ws), cross wind (cw), temperature (t), relative humidity (hr), barometric pressure (bp) and dew point (dp) were recorded using a kestrel(®) 4500 weather station. the median indoor/outdoor fsc was 360/1230 cfu m(-3). fsc correlat ...201424070332
biotransformation and improved enzymatic extraction of chlorogenic acid from coffee pulp by filamentous fungi.the highest enzymatic extraction of covalent linked chlorogenic (36.1%) and caffeic (ca) (33%) acids from coffee pulp (cp) was achieved by solid-state fermentation with a mixture of three enzymatic extracts produced by aspergillus tamarii, rhizomucor pusillus, and trametes sp. enzyme extracts were produced in a practical inexpensive way. synergistic effects on the extraction yield were observed when more than one enzyme extract was used. in addition, biotransformation of chlorogenic acid (cha) b ...201323341203
mycomon-monitoring project of toxigenic fungi in food in years 1999 - 2001.an occurrence of the toxigenic fungi producing aflatoxins and ochratoxin a in food was investigated in the study ("mycomon") in years 1999-2001. twenty five commodities were purchased from retail in twelve collection places in the czech republic (300 food samples together). the presence of potentially toxigenic fungiaspergillus flavus was observed in 28% of the sampled food (black pepper, black tea, caraway seeds, fine flour, fruit tea, oat flakes) in the year 1999, in 17% of the sampled food (b ...200223606161
positive and negative aspects of green coffee consumption - antioxidant activity versus mycotoxins.the quality of coffee depends not only on the contents of healthy compounds but also on its contamination with microorganisms that can produce mycotoxins during development, harvesting, preparation, transport and storage.201728195330
cyclopiazonic acid biosynthesis gene cluster gene cpam is required for speradine a biosynthesis.speradine a is a derivative of cyclopiazonic acid (cpa) found in culture of an aspergillus tamarii isolate. heterologous expression of a predicted methyltransferase gene, cpam, in the cpa biosynthesis gene cluster of a. tamarii resulted in the speradine a production in a 2-oxocpa producing a. oryzae strain, indicating cpam is involved in the speradine a biosynthesis.201526207447
plant-available inorganic nutrient levels are increased in rice-derived distillery effluents inoculated with microbes.the purpose of this study was to assess the effects of microbes on plant-available inorganic nutrients and a phytohormone in rice-derived distillery effluents.201425040155
decatamariic acid, a new mitochondrial respiration inhibitor discovered by pesticidal screening using drug-sensitive saccharomyces cerevisiae.a new decalin, decatamariic acid, was isolated from a cultured broth of the fungus aspergillus tamarii fki-6817. its absolute configuration was elucidated by nmr and electronic circular dichroism. decatamariic acid (10 μm) elicited ~50% inhibition of the atp production in mitochondria isolated from wild-type saccharomyces cerevisiae without affecting the activities of respiratory enzymes. the action manner of this compound may be interesting as a possible seed for new pesticides.201728096549
coconut oil induced production of a surfactant-compatible lipase from aspergillus tamarii under submerged fermentation.filamentous fungi are efficient producers of lipases. the present study focuses on identification of a potent lipolytic fungus and enhancement of lipase production through optimization of nutritional and cultural conditions under submerged fermentation. molecular characterization of the fungus by 18s rdna sequencing revealed its identity as aspergillus tamarii with 98% homology. maximum lipase production was noted in mineral salts medium supplemented with coconut oil (2.5%, v/v). a combination o ...201727709654
response surface optimization of bioremediation of acid black 52 (cr complex dye) using aspergillus tamarii.bioremediation of the cr complex dye (acid black 52) was performed in batch and continuous modes using growing aspergillus tamarii. the removal of cu which may be present as an impurity was 100% at 100 mg/l initial dye concentration. the removal of color and cr decreased from 87% to 4% and from 92% to 8%, respectively, by increasing dye concentration from 100 to 5000 mg/l in batch mode. the removal of color and cr increased from 27% to 67.8% and from 32% to 72%, respectively, with increasing hyd ...201727242169
cytotoxic and antibiotic cyclic pentapeptide from an endophytic aspergillus tamarii of ficus carica.a new cyclic pentapeptide, disulfide cyclo-(leu-val-ile-cys-cys) (1), named malformin e, together with 13 known cyclic dipeptides, was isolated from the culture broth of endophytic fungus fr02 from the roots of ficus carica. the strain fr02 was identified as aspergillus tamarii on the basis of morphological characteristics and molecular analyses of internal transcribed spacer (its). their structures were determined by the combination of 1d and 2d nmr spectroscopy, hrms (esi), uv, and marfey's an ...201627147299
purification and characterization of a surfactant-compatible lipase from aspergillus tamarii jgif06 exhibiting energy-efficient removal of oil stains from polycotton fabric.an extracellular lipase with 23,666.66 u/ml/min activity was produced by aspergillus tamarii jgif06 under submerged fermentation in mineral salt medium containing coconut oil (2.5 % v/v), tryptone (2 % w/v) and ammonium chloride (2 % w/v), with initial ph of 5 ± 0.2, incubated at 25 °c for 7 days on a rotary shaker at 120 rpm. a 7.9-fold increase in lipase-specific activity was recorded after purification by deae sepharose ion exchange and sephadex g200 column chromatography. the apparent molecu ...201628330188
metabolic fate of 3α,5-cycloandrostanes in the endogenous lactonization pathway of aspergillus tamarii kita.a series of 3α,5-cycloandrostane analogues with a range of functionality (6α and 6β alcohols and ketone) at carbon 6 were tested in the endogenous lactonization pathway in aspergillus tamarii kita. this metabolic route converts progesterone to testololactone in high yield through a four step enzymatic pathway. to date, no studies have looked at the effect of steroids devoid of polar functionality at carbon 3 and their subsequent metabolic fate by fungi which contain baeyer-villiger monooxygenase ...201526372080
biomineralization and formulation of endosulfan degrading bacterial and fungal consortiums.microbial degradation offers an effective approach to remove toxicants and in this study, a microbial consortium consisting of bacterial strains and fungal strains were originally obtained from endosulfan contaminated agricultural soils. identification of the bacterial isolates by 16s rrna sequences revealed the isolates to be halophilic bacterium jas4, klebsiella pneumoniae jas8, enterobacter asburiae jas5, and enterobacter cloacae jas7, whereas the fungal isolates were identified by 18s rrna s ...201425454517
extractive fermentation of xylanase from aspergillus tamarii urm 4634 in a bioreactor.of the many reported applications for xylanase, its use as a food supplement has played an important role for monogastric animals, because it can improve the utilisation of nutrients. the aim of this work was to produce xylanase by extractive fermentation in an aqueous two-phase system using aspergillus tamarii urm 4634, increasing the scale of production in a bioreactor, partially characterising the xylanase and evaluating its influence on monogastric digestion in vitro. through extractive ferm ...201424850359
enhanced production of aspergillus tamarii lipase for recovery of fat from tannery fleshings.the influence of various oil cakes has been investigated for high level production of lipase using aspergillus tamarii mtcc 5152. by solid state fermentation in wheat bran containing 2.5% w/w gingili oil cake at 70% v/w moisture content the fungus produced a maximal yield of lipase (758 ± 3.61 u/g) after 5 days of incubation using 2% v/w inoculum containing 10(6) spores/ml. wheat bran and gingili oil cake with supplementation of gingili oil (1.0% w/w), glucose (0.5% w/w) and peptone (0.5% w/w) g ...201324688497
effect of water activity and temperature on the germination and growth of aspergillus tamarii isolated from "maldive fish".germination times and radial growth rates of cyclopiazonic acid producing strains of aspergillus tamarii isolated from a smoked dried fish product were studied over water activities (a(w)) ranging from 0.99 to 0.79 at 25°c, 30°c, 35°c and 40°c on two laboratory media. the a(w) of the media was controlled by either nacl or a mixture of glucose and fructose. the optimum germination and growth were observed at temperatures between 30°c and 35°c. germination was favored at the highest a(w) of 0.99 u ...201223177051
effect of caffeine concentration on biomass production, caffeine degradation, and morphology of aspergillus tamarii.the aim of the present study was to evaluate the effect of the initial caffeine concentration (1-8 g/l) on growth and caffeine consumption by aspergillus tamarii as well as pellet morphology, in submerged fermentation. caffeine was used as sole nitrogen source. at 1 g/l of initial caffeine concentration, caffeine degradation was not affected, resulting in a production of 8.7 g/l of biomass. the highest biomass production (12.4-14.8 g/l) was observed within a range of 2 to 4 g/l of initial caffei ...201323054686
molecular identification of a novel inulinolytic fungus isolated from and grown on tubers of helianthus tuberosus and statistical screening of medium components.of 24 fungi belonging to more than five genera isolated from tubers of rotten helianthus tuberosus, 11-inulinolytic active isolates were able to develop halo zones around their fungal colonies, indicating inulinase activity. alternaria, aspergillus, fusarium, pencillium and trichoderma were the most common inulinolytic genera, representing more than 90 % of the total positive inulinolytic fungi. aspergillus tamarii and pencillium citrinum quantitatively recorded better growth (5.5 and 4.7 mg ml( ...201222892864
antimicrobial activities of polyhexamethylene guanidine hydrochloride-based disinfectant against fungi isolated from cocoa beans and reference strains of bacteria.this study was conducted to assess the antibacterial and the antifungal activity of a polyhexamethylene guanidine hydrochloride (phmgh)-based disinfectant and to determine if it could be used as a disinfectant for the treatment of cocoa beans. the activity of phmgh was tested in vitro for efficacy against five reference strains of pathogenic bacteria and six strains of fungi isolated from cocoa beans. all the strains tested were sensitive to the disinfectant. the mics reported were between 0.01 ...201222691490
impact of aeration and agitation on metabolic heat and protease secretion of aspergillus tamarii in a real-time biological reaction calorimeter.the effects of aeration and agitation on metabolic heat, alkaline protease production and morphology for aspergillus tamarii mtcc5152 are reported in this manuscript. measurement of metabolic heat has been attempted by the continuous and dynamic heat balance method in a biological real-time reaction calorimeter. at lower agitation intensities, growth-related processes were dominating. as a result the protease activity and the product heat yields were lower than those for 350 and 450 rpm. althoug ...201222446792
initial proteome analysis of caffeine-induced proteins in aspergillus tamarii using two-dimensional fluorescence difference gel electrophoresis.caffeine is toxic to most microorganisms. however, some filamentous fungi, such as aspergillus tamarii, are able to metabolize this alkaloid when fed caffeine as the sole nitrogen source. the aim of the present work was to identify intracellular a. tamarii proteins, regulated by caffeine, using fluorescence difference two-dimensional gel electrophoresis. specific proteins from two culture media of a. tamarii grown either on ammonium sulfate or caffeine as the sole nitrogen source were analysed b ...201222391696
distribution of glyoxylate-cycle enzymes between microbodies and mitochondria in aspergillus tamarii.the distribution of glyoxylate-cycle enzymes between microbodies and mitochondria was examined in ethanol-grown aspergillus tamarii kita. particulate activities of catalase and the two glyoxylate by-pass enzymes, malate synthase and isocitrate lyase, were localized in the microbodies. the microbodies had a buoyant density of about 1.23 g cm(-3) after isopycnic centrifugation in linear sucrose gradients. particulate activities of the other two glyoxycitrate synthase, together with that of succina ...197624424991
the application of aqueous biphasic systems as strategy to purify tannase from aspergillus tamarii urm 7115.the aims of the current study are to assess the influence of polyethylene glycol (peg) concentration, molar mass, ph and citrate concentrations on aqueous biphasic systems based on 2(4) factorial designs, as well as to check their capacity to purify tannase secreted by aspergillus tamarii urm 7115. tannase was produced through submerged fermentation at 26 °c for 67 h in czapeck-dox modified broth and added with yeast extract and tannic acid. the factorial design was followed to assess the influe ...201728857720
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