| effects of the fungus aspergillus penicillioides on the house dust mite dermatophagoides pteronyssinus: an experimental re-evaluation. | in this report the widely-held view that house dust mites benefit from fungal contamination of the dietary substratum is re-examined. the performance of dermatophagoides pteronyssinus (acari: pyroglyphidae) is documented over two successive generations in the presence or absence of the xerophilic fungus aspergillus penicillioides (hyphomycetales: moniliaceae). this fungus reduced survival, development rate, adult length and fecundity of d. pteronyssinus. detrimental effects of a. penicillioides ... | 1993 | 8369562 |
| influence of temperature, water activity and ph on growth of some xerophilic fungi. | the combined effects of water activity (aw), ph and temperature on the germination and growth of seven xerophilic fungi important in the spoilage of baked goods and confectionery were examined. eurotium rubrum, e. repens, wallemia sebi, aspergillus penicillioides, penicillium roqueforti, chrysosporium xerophilum and xeromyces bisporus were grown at 25, 30 and 37 degrees c on media with ph values of 4.5, 5.5, 6.5 and 7.5 and a range of water activities (aw) from 0.92 to 0.70. the aw of the media ... | 2003 | 12423914 |
| use of gas chromatography-mass spectrometry/solid phase microextraction for the identification of mvocs from moldy building materials. | gas chromatography-mass spectrometry/solid phase microextraction (gc-ms/spme) was applied to identify microbial volatile organic compounds (mvocs) in water-damaged, mold-infested building materials (gypsum board papers (n=2), mineral wool, and masonite) and in cultivated molds (aspergillus penicillioides, stachybotrys chartarum, and chaetomium globosum). three spme fibers (65-microm pdms-dvb, 75-microm carboxen-pdms, and 70-microm carbowax-stableflex) designed for automated injection were used o ... | 2003 | 12531501 |
| identity of the xerophilic species aspergillus penicillioides: integrated analysis of the genotypic and phenotypic characters. | we examined the identity of aspergillus penicillioides, the typical xerophilic and strictly anamorphic species, using an integrated analysis of the genotypic and phenotypic characters. our experimental methods on two genotypic characters, i.e., dna base composition using the hplc method and dna relatedness using the nitrocellulose filter hybridization technique between a. flavus, a. oryzae, and their close relations revealed a good agreement with the values by buoyant density (for dna base compo ... | 1999 | 12501399 |
| aurantiamide acetate, a selective cathepsin inhibitor, produced by aspergillus penicilloides. | aurantiamide acetate was isolated from the fermentation broth of aspergillus penicilloides for the first time. aurantiamide acetate inhibited cysteine proteinases, in particular, cathepsin l (3.4.22.15) and b (3.4.22.1) with ic50 of 12 microm and 49 microm, respectively. in the adjuvant-arthritic rat model, subcutaneously administered 10 mg/kg body weight of this compound suppressed hind paw swelling. | 2001 | 11440138 |
| optimal efficacy of a fungicide preparation, natamycin, in the control of the house-dust mite, dermatophagoides pteronyssinus. | the efficacy of a fungicidal preparation, natamycin, for the effective control of dermatophagoides pteronyssinus (trouessart), was determined at different concentrations on mattress-dust microsites, including ticking. one treatment with the commercially available natamycin concentration (tymasil) resulted in a 65% reduction of house-dust mite populations within 4 weeks of treatment. the acaricidal control could be proven despite the protective role of mattress fibres such as ticking. however, mi ... | 1988 | 3259916 |
| evidence refuting the contribution of the fungus aspergillus penicillioides to the allergenicity of the house dust mite dermatophagoides pteronyssinus. | this communication demonstrates unequivocally that the fungi associated with house dust mites do not contribute to mite allergenicity. the evidence is twofold: first, larval mites which lack fungi have allergen profiles indistinguishable from fungus-bearing adult mites. second, the allergen profile of experimentally-derived fungus-free adult mites and mites re-fed the fungus aspergillus penicillioides are identical. | 1992 | 1582703 |
| how relevant are house dust mite-fungal interactions in laboratory culture to the natural dust system? | both house dust and house dust mites dermatophagoides pteronyssinus contained a wider range of fungi than laboratory mite cultures. in total, nine species of fungi were isolated from d. pteronyssinus in house dust, and these included three xerophilic species (eurotium amstelodami, aspergillus penicillioides and wallemia sebi) commonly found in laboratory cultures of d. pteronyssinus. it is concluded that mites do interact with a similar range of fungi in natural dust and in laboratory culture, b ... | 1992 | 1493747 |
| aspergillus penicilloides and eurotium halophilicum in association with house-dust mites. | aspergillus penicilloides speg., eurotium halophilicum christensen et al. and other xerophilic fungi were isolated from house dust. the isolates of e. halophilicum are described and compared with the type strain of this species. a. penicilloides appeared to be rather common. the species concept of this species is broadened to accomodate some variable forms with different colony characters and conidophore structures. the role of both species is discussed in connection with house-dust mites, house ... | 1978 | 714145 |
| ecological relationships between xerophilic fungi and house-dust mites (acarida: pyroglyphidae). | at. 75 and 80% relative humidity (rh), on a wheat germ flake medium, aspergillus penicilloides grew abundantly and suppressed the population growth of dermatophagoides pteronyssiunus. at 71% rh, a. penicilloides grew moderately and was only antagonistic to d. pteronyssinus when the fungus was previously incubated on the medium.on a human dander medium and on mattress dust, a. penicilloides grew moderately at 71% and 75% rh and stimulated the development of d. pteronyssinus populations. also a mo ... | 1978 | 28309597 |
| halophilic aspergillus penicillioides from athalassohaline, thalassohaline, and polyhaline environments. | aspergillus penicillioides is a true halophile, present in diverse econiches - from the hypersaline athalassohaline, and thalassohaline environments, to polyhaline systems, and in different geographical locations. twenty seven isolates from these environments, were seen to be moderate halophiles, euryhaline in nature. they had an obligate need of a low aw and were unable to grow on a regular defined medium such as czapek dox agar, as well as on varied nutrient rich agar media such as malt extrac ... | 2014 | 25140168 |
| fungi in ontario maple syrup & some factors that determine the presence of mold damage. | maple syrup is a high value artisanal product produced mainly in canada and a number of states primarily in the northeast usa. mold growth (wallemia sebi) on commercial product was first reported in syrup in 1908. since then, few data have been published. we conducted a systematic examination for fungi in maple syrup from 68 producers from all of the syrup-producing areas of ontario, canada. the mean ph of the samples was ph 6.82, sugar content averaged 68.0±0.89 °brix and aw averaged 0.841±0.01 ... | 2015 | 26001061 |
| purification and characterization of a polyextremophilic α -amylase from an obligate halophilic aspergillus penicillioides isolate and its potential for souse with detergents. | an extracellular α-amylase from the obligate halophilic aspergillus penicillioides tistr3639 strain was produced and enriched to apparent homogeneity by ammonium sulfate precipitation and sephadex g100 gel filtration column chromatography. the mass of the purified amylase was estimated to be 42 kda by sds-page. with soluble starch as the substrate it had a specific activity of 118.42 u · mg(-1) and vmax and km values of 1.05 µmol · min(-1) · mg(-1) and 5.41 mg · ml(-1), respectively. the enzyme ... | 2015 | 26180787 |
| fatal disseminated aspergillus penicillioides infection in a 3-month-old infant with suspected cystic fibrosis: autopsy case report with review of literature. | patients with cystic fibrosis (cf) often are colonized by aspergillus species in their respiratory tract, but invasive aspergillosis is a rare complication. we describe the autopsy findings of an infant with cystic fibrosis who had fatal disseminated aspergillosis. the causative agent was identified as a. penicillioides by molecular technique. to our knowledge, this is the first report of disseminated aspergillosis caused by a. penicillioides in any type of patient. the literature on invasive as ... | 2017 | 26579953 |
| water-, ph- and temperature relations of germination for the extreme xerophiles xeromyces bisporus (frr 0025), aspergillus penicillioides (jh06thj) and eurotium halophilicum (frr 2471). | water activity, temperature and ph are determinants for biotic activity of cellular systems, biosphere function and, indeed, for all life processes. this study was carried out at high concentrations of glycerol, which concurrently reduces water activity and acts as a stress protectant, to characterize the biophysical capabilities of the most extremely xerophilic organisms known. these were the fungal xerophiles: xeromyces bisporus (frr 0025), aspergillus penicillioides (jh06thj) and eurotium hal ... | 2017 | 27562192 |
| aspergillus penicillioides differentiation and cell division at 0.585 water activity. | water availability acts as the most stringent constraint for life on earth. thus, understanding the water relations of microbial extremophiles is imperative to our ability to increase agricultural productivity (e.g., by enhancing the processing and turnover of dead organic matter in soils of arid regions), reduce human exposure to mycotoxins in buildings and our food-supply chain, prevent the spoilage of foods/animal feeds, books, museum specimens and artworks and better control microbiology of ... | 2017 | 27871132 |
| glycerol enhances fungal germination at the water-activity limit for life. | for the most-extreme fungal xerophiles, metabolic activity and cell division typically halts between 0.700 and 0.640 water activity (approximately 70.0-64.0% relative humidity). here, we investigate whether glycerol can enhance xerophile germination under acute water-activity regimes, using an experimental system which represents the biophysical limit of earth's biosphere. spores from a variety of species, including aspergillus penicillioides, eurotium halophilicum, xerochrysium xerophilum (form ... | 2017 | 27631633 |
| an impedance-based mold sensor with on-chip optical reference. | a new miniaturized sensor system with an internal optical reference for the detection of mold growth is presented. the sensor chip comprises a reaction chamber provided with a culture medium that promotes the growth of mold species from mold spores. the mold detection is performed by measuring impedance changes with integrated electrodes fabricated inside the reaction chamber. the impedance change in the culture medium is caused by shifts in the ph (i.e., from 5.5 to 8) as the mold grows. in ord ... | 2016 | 27690039 |
| molecular characterization of microbial communities in bioaerosols of a coal mine by 454 pyrosequencing and real-time pcr. | microbial diversity and abundance in bioaerosols of a coal mine were analyzed based on 454 pyrosequencing and real-time polymerase chain reaction (pcr). a total of 37,191 high quality sequences were obtained and could be classified into 531, 1730 and 448 operational taxonomic units respectively for archaea, bacteria and fungi at 97% sequence similarity. the shannon diversity index for archaea, bacteria and fungi was respectively 4.71, 6.29 and 3.86, indicating a high diversity in coal mine bioae ... | 2015 | 25872733 |
| is there a common water-activity limit for the three domains of life? | archaea and bacteria constitute a majority of life systems on earth but have long been considered inferior to eukarya in terms of solute tolerance. whereas the most halophilic prokaryotes are known for an ability to multiply at saturated nacl (water activity (a(w)) 0.755) some xerophilic fungi can germinate, usually at high-sugar concentrations, at values as low as 0.650-0.605 a(w). here, we present evidence that halophilic prokayotes can grow down to water activities of <0.755 for halanaerobium ... | 2014 | 25500507 |
| assessment of home environments with a fungal index using hydrophilic and xerophilic fungi as biologic sensors. | abstract previously, the author proposed a 'fungal index' that quantifies the capacity for fungal growth in a test environment where a device (fungal detector) encapsulating spores of a xerophilic sensor fungus eurotium herbariorum was placed. it was also found that an extremely xerophilic fungus, aspergillus penicillioides, was suitable as a sensor fungus at sites with lower relative humidity (rh). in this report, the hydrophilic fungus alternaria alternata was added to sensor fungi for the de ... | 2011 | 21995759 |
| effects of biological control of rice weevil by anisopteromalus calandrae with a population of two aspergillus spp. | in this study, we assessed the interaction occurring between the rice weevil and two storage molds (aspergillus candidus link and aspergillus niger van tiegem) that prefer different moisture regimens under rice storage conditions. rice weevil induced rapid population growth in both of the storage molds. the colony forming units (cfus) of a. candidus and a. niger peaked at 10(9.00 +/- 0.02) and 10(7.72 +/- 0.03)/g, respectively. all of the rice grains were infested with the molds and eventually d ... | 2009 | 19791605 |
| microbial content of house dust samples determined with qpcr. | this study was designed to produce information about microbial concentrations using qpcr and their variation in different seasons and home environments with analyses of two types of house dust samples. also the correlations between the two types of samples and the reproducibility of the parallel subsamples were studied. two types of vacuumed house dust samples, rug dust and vacuum cleaner bag dust, were collected in 5 normal urban homes in four different seasons (n=20+20). from all dust samples, ... | 2009 | 19473690 |
| comparison of mold concentrations quantified by msqpcr in indoor and outdoor air sampled simultaneously. | mold specific quantitative pcr (msqpcr) was used to measure the concentrations of the 36 mold species in indoor and outdoor air samples that were taken simultaneously for 48 h in and around 17 homes in cincinnati, ohio. the total spore concentrations of 353 per m(3) of indoor air and 827 per m(3) of outdoor air samples were significantly different (p<or=0.05). however, only the concentrations of aspergillus penicillioides, cladosporium cladosporioides types 1 and 2 and cladosporium herbarum were ... | 2007 | 17467772 |
| two new dioxopiperazine derivatives, arestrictins a and b, isolated from aspergillus restrictus and aspergillus penicilloides. | in the course of searching for bioactive substances, two new dioxopiperazine derivatives, arestrictins a (1) and b (2), were isolated along with the known dioxopiperazine, cristatin a (3), and the known peptide, asperglucide (4), from the organic extract of the xerophilic fungi, aspergillus restrictus and aspergillus penicilloides. the absolute structures of 1 and 2, except for the configuration of the secondary alcohol in 1, were established by spectroscopic and chemical investigation. the abso ... | 2006 | 17139096 |
| [moulds and yeasts in bottled water and soft drinks]. | some damaged cartons of soft drinks and carbonated water were analyzed to detect the microorganisms that caused the damage. the contaminants of sugar used in the production of one of the drinks were also studied. the methods of déak & beuchat and pitt & hocking were used for the identification of yeasts and moulds, respectively. the agents of the spoilage of soft drinks were debaryomyces hansenii, debaryomyces polymorphus, galactomyces geotrichum, metschnikowia pulcherrima, mucor circinelloides, ... | 2006 | 17037258 |
| fungal bioconversion of 2,4-dichlorophenoxyacetic acid (2,4-d) and 2,4-dichlorophenol (2,4-dcp). | ninety strains of fungi from the collection of our mycology laboratory were tested in galzy and slonimski (gs) synthetic liquid medium for their ability to degrade the herbicide 2,4-dichlorophenoxyacetic acid (2,4-d) and its by-product, 2,4-dichlorophenol (2,4-dcp) at 100 mg l(-1), each. evolution of the amounts of each chemical in the culture media was monitored by hplc. after 5 days of cultivation, the best results were obtained with aspergillus penicilloides and mortierella isabellina for 2,4 ... | 2005 | 16201028 |
| identification of aspergillus fumigatus and related species by nested pcr targeting ribosomal dna internal transcribed spacer regions. | aspergillus fumigatus is the most common species that causes invasive aspergillosis. in order to identify a. fumigatus, partial ribosomal dna (rdna) from two to six strains of five different aspergillus species was sequenced. by comparing sequence data from genbank, we designed specific primer pairs targeting rdna internal transcribed spacer (its) regions of a. fumigatus. a nested pcr method for identification of other a. fumigatus-related species was established by using the primers. to evaluat ... | 2001 | 11376067 |