| aspergillus flavipes group osteomyelitis. | lumbar vertebral osteomyelitis caused by aspergillus flavipes group organisms developed in a nonchronically immunosuppressed patient. diagnosis was confirmed morphologically and culturally from both closed needle biopsy of the vertebrae and subsequent lumbar laminectomy. the patient was treated with 3 g of amphotericin b with apparent eradication of the organism. | 1979 | 443955 |
| two metabolites from aspergillus flavipes. | two novel fungal metabolites, n-benzoyl-l-phenylalaninol (1a) and asperphenamate (2) were isolated from the culture filtrate and mycelium of aspergillus flavipes atcc 11013. n-benzoyl-l-phenylalaninol was identified by direct comparison with an authentic sample. the structure of asperphenamate is proposed as (s)-n-benzoyl-phenylalanine-(s)-2-benzamido-3-phenyl propyl ester, based on chemical and spectroscopic evidence. | 1977 | 875642 |
| chemical and structural similarities in wall polysaccharides of some penicillium, eupenicillium and aspergillus species. | various fractions were extracted from cell-wall material of eupenicillium crustaceum, penicillium brevi-compactum, p. decumbens, aspergillus flavipes and a. ochraceus. the most characteristic fractions, which may have chemotaxonomic relevance, were f1i, an alpha-(1-3) glucan (alkalisoluble, water-insoluble), which amounted to 16.2-32.5% of the cell-wall material, and f1s (alkali and water-soluble) which represented 2.5-6.2% of the cell-wall material and was identified as a beta-(1-5) galactan. 1 ... | 1992 | 1537545 |
| separation and some properties of an endo-1,4-beta-d-xylanase from aspergillus flavipes. | a fungal strain isolated from stored wheat straw and identified as aspergillus flavipes was found to produce an extracellular endo-1,4,beta-d-xylanase when grown in shake-culture of a mineral salt medium containing 0.1% wheat strawxylan as a sole source of carbon for 3 days at 28 degrees c. the enzyme was separated by precipitation with ammonium sulphate and desalted by sephadex g-25 column. fractionation and purification of the enzyme were carried out by chromatography on bio-gel p-100. the pur ... | 1990 | 2100903 |
| [a case of bronchopulmonary aspergillosis recurring in a residual tuberculous cavity]. | a 52-year-old man, who had undergone right upper lobectomy because of active tuberculosis 29 years before, was admitted with complaints of severe cough and expectation. two years ago, he had pulmonary aspergillosis and was successfully treated with some anti-mycotic agents. this time his chest x-p showed fungus ball in a residual tuberculous cavity in the right upper field and he was diagnosed as pulmonary aspergilloma from the results of radiological findings, sputum culture, and serologic test ... | 1989 | 2811014 |
| humoral immunity in disseminated aspergillus terreus infection in the dog. | aspects of humoral immunity were studied in 17 dogs with disseminated aspergillosis (16 cases aspergillus terreus, 1 case aspergillus flavipes). all dogs had markedly raised serum igg levels by single radial immunodiffusion (range 1500-6000 mg dl-1). despite this, serum antibody to a. terreus was demonstrated in only 7/16 cases by agar gel diffusion, 9/16 cases by counter immunoelectrophoresis, 10/16 by elisa and 11/16 by an indirect immunofluorescence assay. serum antibody was also detected in ... | 1988 | 3131949 |
| microbial transformation studies on arteannuin b. | the microbial transformation of the sesquiterpene lactone arreannuin b [3] using aspergillus flavipes produced dihydroarteannuin b [4] as the main transformation product. preparative-scale fermentation of 3 with beauveria bassiana, on the other hand, has resulted in the production of two metabolites, 3 beta-hydroxyarteannuin b [5] and 13-hydroxy-11-epi-dihydroarteannuin b [6]. the structure of these metabolites, all of which are new compounds, was established using chemical and spectroscopic tec ... | 1987 | 3437286 |
| high field and 2d-nmr studies with the aporphine alkaloid glaucine. | the aporphine alkaloid glaucine (1) was examined by comparison of the high field (600 mhz) 1h-nmr spectra of 1 vs. racemic 6a,7,7-trideutereoglaucine (4,5), by computer-simulated 1h-nmr spectra at 600 mhz, by using decoupled proton spectra, and two-dimensional cosy and hetcor experiments with 1 at 500 and 360 mhz, respectively, and using high field (90 mhz) 13c-nmr of s-(+)-glaucine (1). emphasis was placed on the resolution of the chemical shifts and coupling constants for the h-4 alpha, h-4 be ... | 1986 | 3783155 |
| isolation of the first trypsin inhibitor from the genus aspergillus. | the fungus aspergillus flavipes was grown on a czapeck sucrose medium; the biomass so obtained was treated with high concentration of sucrose to release intracellular metabolites. sephadex g-75 chromatography of the latter yielded a pure protein having anti-trypsin activity in vitro. | 1985 | 4063473 |
| flavipucine (3'-isovaleryl-6-methylpyridine-3-spiro-2'-oxiran-2(1h),-4(3h)-dione), an antibiotic from aspergillus flavipes. | | 1972 | 4627703 |
| isolation & purification of a toxic pigment from the culture filtrate of aspergillus flavipes. | | 1972 | 4658190 |
| [glutamycin: a new antibiotic produced by a strain of aspergillus flavipes]. | | 1969 | 5398540 |
| a new antibiotic produced by a strain of aspergillus flavipes. | | 1968 | 5651015 |
| [coordination immobilization of fungal proteinases]. | the possibility of coordination immobilization of fungal enzymes from aspergillus niger mb and aspergillus flavipes on the styrene copolymer with maleic acid has been explored. optimal conditions for preparing insoluble chromium-containing enzyme polymeric complexes have been studied. the synergistic effect of salts of different metals on stability of chromium-containing immobilized preparations has been demonstrated. properties of the resultant immobilized preparations have been examined. | 1981 | 7017673 |
| structure determination, pharmacological evaluation, and structure-activity studies of a new cyclic peptide substance p antagonist containing the new amino acid 3-prenyl-beta-hydroxytyrosine, isolated from aspergillus flavipes. | two novel cyclic heptapeptides, peptides 1a and 1c, were isolated from an aspergillus flavipes culture, originally isolated from soil, and their structures established by chemical and spectroscopic evidence. peptide 1a contains a new amino acid, 3-prenyl-beta-hydroxytyrosine, and is a competitive antagonist to substance p at the human nk1 receptor, with an inhibitor affinity constant (ki) of 8 +/- 4 microm. methylation of 1a gave the monomethyl derivative 1b, which is a more potent competitive a ... | 1994 | 7508512 |
| spiroquinazoline, a novel substance p inhibitor with a new carbon skeleton, isolated from aspergillus flavipes. | a novel substance p inhibitor, spiroquinazoline [1], was isolated from the fungus aspergillus flavipes, which was originally obtained from soil. the structure of 1 was determined by analysis of spectroscopic data and 1 was shown to contain a new carbon skeleton containing a spiro-carbon center. also isolated from the same culture extract were the new natural product, benzodiazepiedione [3], and the known compounds, acyl aszonalenin [4], n-benzoyl-l-phenylalaninol, and seven diketopiperazines. | 1994 | 7517439 |
| flavipucine and brunnescin, two antibiotics from cultures of the mycophilic fungus cladobotryum rubrobrunnescens. | two antimicrobial metabolites were isolated from submerged cultures of cladobotryum rubrobrunnescens, a mycophilic fungus growing on a inocybe species. one of the compounds proved to be identical to flavipucine (2), an antibiotic previously isolated from aspergillus flavipes (casinovi et al., 1968) and from a macrophoma species (sassa t. and onuma y. (1983), agric. biol. chem. 47, 1155-1157). the other metabolite, brunnescin (1), is a new tetrasubstituted furan derivative which exhibits antibact ... | 1995 | 7546030 |
| purification and some properties of tyrosinase from aspergillus flavipes 56003 | a method for isolation and purification of tyrosinase from the fungus aspergillus flavipes 56003 was developed. the method includes extraction with water, concentration on deae-cellulose, gel-filtration on acrylex p-150, and ion-exchange chromatography on deae-toyopearl 650m. the tyrosinase was purified to apparent homogeneity according polyacrylamide gel electrophoresis and ultracentrifugation. the tyrosinase is a 130-kd protein with pi 4.6. it contains two copper atoms. the km and vmax for tyr ... | 1999 | 10231595 |
| tmc-169, a new antibiotic of the aspochalasin group produced by aspergillus flavipes. | | 1999 | 10470683 |
| use of the 6-methylsalicylic-acid-synthase gene as a discriminating marker between aspergillus terreus and aspergillus flavipes. | in nineteen pathogenic and saprophytic isolates denoted as aspergillus terreus the presence and restriction pattern of the gene pksm (6-methylsalicylic acid synthase) was determined. five patterns (a-e) were found and in three isolates the gene was missing. the rapd (random amplified polymorphic dna) patterns with three primers were analyzed. the strains with pksm pattern possibly derived from the most common pattern a by single mutation (patterns b-d) were also related by rapd. among them, thre ... | 1999 | 10997132 |
| study of the kinetics of oxidation of monophenols by tyrosinase. the effect of reducers. | kinetics of oxidation of monophenols by tyrosinase from the fungus aspergillus flavipes 56003 and the effect of fe2+, serine, and ascorbic acid on this reaction were studied. the effectors were shown to accelerate the oxidation of monophenols, decreasing the lag-time of the reaction. it is assumed that the activation of the tyrosinase in the presence of fe2+ is due to a direct reduction of the active site copper ions. serine and ascorbic acid are supposed to affect the reaction of quinone transf ... | 2002 | 11952426 |
| [amylases of the fungus aspergillus flavipes associated with fucus evanescens]. | a promising producer of extracellular amylases, aspergillus flavipes, was selected from 245 strains of marine fungi. depending on the conditions of growth, this strain produced diverse amylolytic complexes. when grown on medium containing peptone and yeast extract (ph 7.0), a. flavipes synthesized three forms of amylase, differing in ph optimum (5.5, 6.0, and 7.5). a single form of the enzyme was synthesized either in the absence of peptone from the medium or at the initial ph value of the mediu ... | 2002 | 11962212 |
| comparison of the sensititre yeastone colorimetric antifungal panel and etest with the nccls m38-a method to determine the activity of amphotericin b and itraconazole against clinical isolates of aspergillus spp. | the evaluation of the sensititre yeastone and etest methods for determining susceptibility to amphotericin b and itraconazole by comparing the mics obtained by these methods at different times of reading with those of the m38-a broth microdilution method. | 2003 | 12917236 |
| studies in the biochemistry of micro-organisms. 97. flavipin, a crystalline metabolite of aspergillus flavipes (bainier & sartory) thom & church and aspergillus terreus thom. | | 1956 | 13341894 |
| production and characterization of alpha-galactosidase from aspergillus flavipes. | an extracellular alpha-galactosidase from the culture filtrate of aspergillus flavipes grown on melibiose as a carbon source was partially purified by hydroxylapatite and diethylaminoethylcellulose chromatographies. electrophoretic analysis showed protein bands corresponding to alpha-galactosidase and invertase activities. the optimum ph and temperature were determined as 4.5-5.0 and 45 degrees c, respectively. the km value for p-nitrophenyl-alpha-d-galactopyranoside was found to be 1.89 mm. the ... | 2003 | 14624479 |
| aspochalasins i, j, and k: three new cytotoxic cytochalasans of aspergillus flavipes from the rhizosphere of ericameria laricifolia of the sonoran desert. | bioassay-guided fractionation of a cytotoxic etoac extract of aspergillus flavipes occurring in the rhizosphere of ericameria laricifolia resulted in the isolation of three new cytochalasans, namely, aspochalasins i (1), j (2), and k (3), and four known cytochalasans, aspochalasins c (4), d (5), and e (6) and tmc-169 (7). the structures of compounds 1-3 were established on the basis of extensive 1d and 2d nmr spectroscopic analysis. all compounds exhibited weak to moderate cytotoxicity against n ... | 2004 | 15043404 |
| cerebroside analogues from marine-derived fungus aspergillus flavipes. | from the mycelium of the marine-derived fungus aspergillus flavipes, isolated from the sea anemone anthopleura xanthogrammica, two new cerebroside analogues, namely flavicerebrosides a (1): [(2s,2'r,3r,4e,8e)-n-2'-hydroxyoctadecanoyl-1-o-beta--galactopyranosyl-9-methyl-4,8-sphingadienine], and b (2): [(2s,2'r,3r,3'e,4e,8e)-n-2'-hydroxy-3'-octadecenoyl-1-o-beta--galactopyranosyl-9-methyl-4,8-sphingadienine], together with two known glycosphingolipids cerebrosides d (3) and c (4), were isolated. t ... | 2004 | 15621583 |
| assessment of ribosomal large-subunit d1-d2, internal transcribed spacer 1, and internal transcribed spacer 2 regions as targets for molecular identification of medically important aspergillus species. | molecular approaches are now being developed to provide a more rapid and objective identification of fungi compared to traditional phenotypic methods. ribosomal targets, especially the large-subunit rna gene (d1-d2 region) and internal transcribed spacers 1 and 2 (its1 and its2 regions), have shown particular promise for the molecular identification of some fungi. we therefore conducted an assessment of these regions for the identification of 13 medically important aspergillus species: aspergill ... | 2005 | 15872227 |
| a novel aspochalasin with hiv-1 integrase inhibitory activity from aspergillus flavipes. | a novel aspochalasin, aspochalasin l (1), was isolated from the fermentation broth of a soil-derived fungal culture identified as aspergillus flavipes (deuteromycota). structure elucidation of 1 was accomplished by detailed spectroscopic data analyses and by comparison with related cytochalasins. aspochalasin l demonstrated activity against hiv integrase with an ic50 of 71.7microm. | 2005 | 15981416 |
| evolutionary relationships among aspergillus terreus isolates and their relatives. | aspergillus terreus is a ubiquitous fungus in our environment. it is an opportunistic human pathogen and economically important as the main producer of lovastatin, a cholesterol lowering drug. our aim was to examine the genetic variability of a. terreus and closely related species using molecular and analytical techniques. lovastatin production was examined by hplc. lovastatin was produced by seven isolates belonging to the species a. terreus. rapd analyses were carried out using 25 different ra ... | 2005 | 16096690 |
| microbiological systems in organic synthesis: preparative-scale resolution of (rs)-glaucine by fusarium solani and stereospecific oxidation of (r)-(-)-glaucine by aspergillus flavipes. | the destructive resolution of (6ar,s)-glaucine (ic) was accomplished by oxidation of the (6as)-(+)-enantiomer (ia), using fusarium solani atcc 12823 to yield the unnatural alkaloid (6ar)-(-)-glaucine (ib). eighteen cultures were examined for their ability to metabolize the (6ar)-(-)-enantiomer (ib), and aspergillus flavipes atcc 1030 was found to catalyze the stereoselective oxidation of this substrate to didehydroglaucine. thus, it has been demonstrated that "r" and "s" organisms exist with reg ... | 1981 | 16345776 |
| [a case of chronic necrotizing pulmonary aspergillosis due to aspergillus flavipes associated with pulmonary fibrosis]. | the patient was a 76-year-old man with complaints of fever, productive cough, and purulent sputum. he was on home oxygen therapy for pulmonary fibrosis. antibiotics were administered, but with no improvement. because the inflammatory condition persisted, the patient was admitted. computed tomographic (ct) scans of the chest demonstrated infiltrative shadows and a fungus ball, and pulmonary aspergillosis was suspected. the patient was given an antimycotic agent and his symptoms gradually resolved ... | 1999 | 18217319 |
| phylogenetic analysis of aspergillus species using dna sequences from four loci. | dna sequences were determined for beta tubulin (bt2), calmodulin (cf), its and lsu rdna (id) and rna polymerase ii (rpb2) from ca. 460 aspergillus isolates. rpb2 and rdna sequences were combined and analyzed to determine relationships in the genus and in the family trichocomaceae. eupenicillium species form a statistically supported clade with origins among the aspergillus clades. a. crystallinus, a. malodoratus and h. paradoxus are members of the eupenicillium clade. a. zonatus, a. clavatoflvus ... | 2008 | 18595197 |
| l-methioninase production by filamentous fungi: i-screening and optimization under submerged conditions. | findings show 21 fungal isolates belonging to eight genera recovered from egyptian soils that have the potential to attack l-methionine under submerged conditions. aspergillus flavipes had the most methioninolytic activity, giving the highest yield of l-methioninase (10.78 u/mg protein), rate of methionine uptake (93.0%), and growth rate (5.0 g/l), followed by scopulariopsis brevicaulis and a. carneus. the maximum l-methioninase productivity (11.60 u/mg protein) by a. flavipes was observed using ... | 2009 | 19048340 |
| l-methioninase production by aspergillus flavipes under solid-state fermentation. | solid-state fermentation was carried out for the production of extra-cellular l-methioninase by aspergillus flavipes (bain and sart.) using nine agro-industrial residues, namely wheat bran, rice bran, wheat flour, coconut seeds, cotton seeds, ground nut cake, lentil hulls, soya beans and chicken feathers. chicken feathers were selected as solid substrate for l-methioninase production by a. flavipes. the maximum l-methioninase productivity (71.0 u/mg protein) and growth (11 mg protein/ml) of a. f ... | 2009 | 19455514 |
| isolation and identification of fr198248, a hydroxylated 1,3-dihydroisobenzofuran, from aspergillus flavipes as an inhibitor of peptide deformylase. | two highly hydroxylated 1,3-dihydroisobenzofurans, fr198248 (1) and fr202306 (2), were isolated as peptide deformylase (pdf) inhibitors from aspergillus flavipes. compounds 1 and 2 inhibited staphylococus aureus pdf with ic(50) values of 3.6 and 2.5 microm, respectively, and also showed antibacterial activity with an mic value of 25 microg/ml. in contrast, 6-o-methyl derivative 3 of compound 2 was inactive against both pdf and s. aureus. | 2010 | 20139618 |
| purification and characterization of a new l-methioninase from solid cultures of aspergillus flavipes. | l-methioninase was purified to electrophoretic homogeneity from cultures of aspergillus flavipes using anion-exchange and gel filtration chromatography by 12.1 fold compared to the crude enzyme preparation. the purified enzyme had a molecular mass of 47 kda under denaturing conditions and an isoelectric point of 5.8 with no structural glycosyl residues. the enzyme had optimum activity at ph 7.8 and ph stability from 6.8-8.0 at 35°c. the enzyme appeared to be catalytically stable below 40°c. the ... | 2011 | 21369990 |
| use of fruit residues for pectinases production by aspergillus flavipes fp-500 and aspergillus terreus fp-370. | aim: utilization of fruit residues for pectinase production by two aspergillus strains for recognizing the effects of some factors during fermentation and describe enzyme production kinetics. methods and results: pectinase production on several fruit residues was compared. the effects of three factors on the production of several pectinases were evaluated by a full factorial 2k experimental design. higher activities were obtained on lemon peel. in both strains acidic ph values and high carbon ... | 2011 | 21623849 |
| asperflavipine a: a cytochalasan heterotetramer uniquely defined by a highly complex tetradecacyclic ring system from aspergillus flavipes qcs12. | asperflavipines a (1) and b (2), two structurally complex merocytochalasans, were isolated from aspergillus flavipes. asperflavipine a (1), which contains two cytochalasan moieties and two epicoccine moieties, is the first cytochalasan heterotetramer to be discovered. it is uniquely defined by 5/6/11/5/6/5/6/5/6/5/5/11/6/5 fused tetradecacyclic rings with three continuous bridged ring systems. asperflavipine b (2) is a cytochalasan heterotrimer containing a cytochalasan and two epicoccine moieti ... | 2017 | 28378450 |
| flavichalasines a-m, cytochalasan alkaloids from aspergillus flavipes. | two new tetracyclic cytochalasans, flavichalasines a and b (1 and 2), three new pentacyclic cytochalasans, flavichalasines c-e (3-5), and eight new tricyclic cytochalasans, flavichalasines f-m (6-13), together with eight known analogues (14-21), were isolated from the solid culture of aspergillus flavipes. structures of these new compounds were elucidated on the basis of extensive spectroscopic analyses including 1d, 2d nmr and hresims data. their absolute configurations were determined by compa ... | 2017 | 28205583 |
| α-glucosidase inhibitors from the marine-derived fungus aspergillus flavipes hn4-13. | three new butenolide derivatives, flavipesolides a-c (1-3), along with 13 known compounds (4-13, aspulvinone q, monochlorosulochrin, and dihydrogeodin), were isolated from the marine-derived aspergillus flavipes hn4-13 from a lianyungang coastal sediment sample. the structures were elucidated by spectroscopic evidence. compounds 4-6 and 9 were noncompetitive α-glucosidase inhibitors with ki/ic50 values of 0.43/34, 2.1/37, 0.79/19, and 2.8/90 μm, respectively. compounds 1-3, 8, 10, and 13 are mix ... | 2016 | 27933892 |
| scaling-up and ionic liquid-based extraction of pectinases from aspergillus flavipes cultures. | the viability of the scaling-up of pectinases production by aspergillus flavipes at 5l-bioreactor scale has been demonstrated by keeping constant the power input, and a drastic increase in the endo- and exopectinolytic enzyme production was recorded (7- and 40-fold, respectively). the main process variables were modelled by means of logistic and gompertz equations. in order to overcome the limitations of the conventional downstream strategies, a novel extraction strategy was proposed on the basi ... | 2017 | 27912181 |
| molecular and spectroscopic characterization of aspergillus flavipes and pseudomonas putida l-methionine γ-lyase in vitro. | pseudomonas putida l-methionine γ-lyase (ppmgl) has been recognized as an efficient anticancer agent, however, its antigenicity and stability remain as critical challenges for its clinical use. from our studies, aspergillus flavipes l-methionine γ-lyase (afmgl) displayed more affordable biochemical properties than ppmgl. thus, the objective of this work was to comparatively assess the functional properties of afmgl and ppmgl via stability of their internal aldimine linkage, tautomerism of pyrido ... | 2016 | 27796875 |
| hot off the press. | a personal selection of 33 recent papers is presented covering various aspects of current developments in bioorganic chemistry and novel natural products, such as epicochalasine a from aspergillus flavipes. | 2016 | 27173803 |
| nanosilver-marine fungal chitosan as antibiotic synergizers against sepsis fish bacteria. | mortality is highly variable within population of cultured fish due to virulent bacteria causing fish septicemia. the use of nano-silver marine fungal chitosan as antibiotic synergisers could be an alternative in the treatment of sepsis fish pathogens. | 2015 | 26885333 |
| epicochalasines a and b: two bioactive merocytochalasans bearing caged epicoccine dimer units from aspergillus flavipes. | two bioactive merocytochalasans, epicochalasines a (1) and b (2), a new class of cytochalasans bearing unexpected scaffolds consisting of fused aspochalasin and epicoccine dimer moieties, were isolated from the liquid culture broth of aspergillus flavipes. both 1 and 2 possess a hendecacyclic 5/6/11/5/6/5/6/5/6/6/5 ring system containing an adamantyl cage and as many as 19 stereogenic centers; however, the fusion patterns of 1 and 2 differ greatly, thus resulting in different carbon skeletons. t ... | 2016 | 26836964 |
| induced production of cytochalasans in co-culture of marine fungus aspergillus flavipes and actinomycete streptomyces sp. | abstarct secondary metabolites profiles of co-culture of aspergillus flavipes and streptomyces sp. that isolated from the same habitat showed an induced production of a series of cytochalasans (five aspochalasins and rosellichalasin, determined by ms and nmr analysis). these cytochalasans were found to be produced by a. flavipes in lc-ms comparison analysis, and biological activity assays revealed that they were able to cause cytotoxic effects against streptomyces sp. within a wide range of conc ... | 2016 | 26783945 |
| novel inexpensive fungi proteases: production by solid state fermentation and characterization. | a comparative study was carried out for proteases production using agroindustrial residues as substrate for solid state fermentation (ssf) of several fungal strains. high protease production was observed for most of the microorganisms studied, as well as very different biochemical characteristics, including activities at specific temperatures and a wide range of ph values. the enzymes produced were very different regarding optimum ph and they showed stability at 50 °c. aspergillus oryzae showed ... | 2016 | 26769513 |
| polyketide butenolide, diphenyl ether, and benzophenone derivatives from the fungus aspergillus flavipes pj03-11. | five new polyketides including three new butenolides (1-3), one new diphenyl ether (4), and one new benzophenone (5), together with eleven known compounds (6-16) were isolated from a wetland fungus aspergillus flavipes pj03-11. their structures were elucidated on the basis of detailed spectroscopic analysis. all the isolated compounds were tested for their α-glucosidase inhibitory activities. the results showed that compounds 1-3, 6, 7, 11, 15 and 16 exhibited stronger inhibitory activities than ... | 2016 | 26706176 |
| transcriptional and proteomic profiling of aspergillus flavipes in response to sulfur starvation. | aspergillus flavipes has received considerable interest due to its potential to produce therapeutic enzymes involved in sulfur amino acid metabolism. in natural habitats, a. flavipes survives under sulfur limitations by mobilizing endogenous and exogenous sulfur to operate diverse cellular processes. sulfur limitation affects virulence and pathogenicity, and modulates proteome of sulfur assimilating enzymes of several fungi. however, there are no previous reports aimed at exploring effects of su ... | 2015 | 26633307 |
| asperchalasine a, a cytochalasan dimer with an unprecedented decacyclic ring system, from aspergillus flavipes. | asperchalasine a (1), the first cytochalasan dimer featuring a unique decacyclic 5/6/11/5/5/6/5/11/6/5 ring system consisting of 20 chiral centers, was isolated from the culture broth of aspergillus flavipes. three biogenetically related intermediates, asperchalasines b-d (2-4), were also isolated. their structures, including their absolute configurations, were elucidated using a combination of hresims, nmr, ecd, molecular modeling, and single-crystal x-ray diffraction techniques. compound 1, wh ... | 2015 | 26334166 |
| batch and pulsed fed-batch cultures of aspergillus flavipes fp-500 growing on lemon peel at stirred tank reactor. | aspergillus flavipes fp-500 grew up on submerged cultures using lemon peel as the only carbon source, developing several batch and pulsed fed-batch trials on a stirred tank reactor. the effect of carbon source concentration, reducing sugar presence and initial ph on exopectinase and endopectinase production, was analyzed on batch cultures. from this, we observed that the highest substrate concentration favored biomass (x max) but had not influence on the corresponding specific production (q p) o ... | 2015 | 26304128 |
| new meroterpenoids from the endophytic fungus aspergillus flavipes ail8 derived from the mangrove plant acanthus ilicifolius. | four new meroterpenoids (2-5), along with three known analogues (1, 6, and 7) were isolated from mangrove plant acanthus ilicifolius derived endophytic fungus aspergillus flavipes. the structures of these compounds were elucidated by nmr and ms analysis, the configurations were assigned by cd data, and the stereochemistry of 1 was confirmed by x-ray crystallography analysis. a possible biogenetic pathway of compounds 1-7 was also proposed. all compounds were evaluated for antibacterial and cytot ... | 2015 | 25574738 |
| revision of aspergillus section flavipedes: seven new species and proposal of section jani sect. nov. | aspergillus section flavipedes contains species found worldwide in soils and rhizospheres, indoor and cave environments, as endophytes, food contaminants and occasionally as human pathogens. they produce many extensively studied bioactive secondary metabolites and biotechnologically relevant enzymes. the taxa were revised based on phylogenetic analysis of sequences from four loci (β-tubulin, calmodulin, rpb2, its rdna), two pcr fingerprinting methods, micro- and macromorphology and physiology. s ... | 2015 | 25344259 |
| coimmobilization of l-methioninase and glutamate dehydrogenase: novel approach for l-homoalanine synthesis. | l-homoalanine, a nonnatural amino acid that is rarely found in human and microorganisms, is used in the synthesis of various medically pivotal antiepileptic drugs and antituberculosis compounds. l-homoalanine can be synthesized by different enzymatic approaches. in this article, the synthesis of l-homoalanine from l-methionine was explored by coimmobilization of aspergillus flavipes l-methioninase (afmetase) and glutamate dehydrogenase (gdh) on polyacrylamide and chitosan. polyacrylamide coimmob ... | 2015 | 25273833 |
| optimisation of environmental conditions for enhanced production of fungal exopectinase using agro-industrial wastes. | management of household solid waste and agro industrial residues generated from various sources is a serious problem due to huge ever increasing population and pollution. application of these worthless agro waste materials to generate a commercially valuable product, pectinase enzyme, using locally isolated fungal strain, aspergillus flavipes, was the main motive of this study. physiological characterisation and enzyme profile determination were done along with formulation of production media. f ... | 2014 | 26563086 |
| a new cyclopeptide metabolite of marine gut fungus from ligia oceanica. | a new cyclopeptide, together with three known amino acid derivatives, was isolated from marine-derived fungus aspergillus flavipes, which was found in the gut of isopod ligia oceanica. the novel peptide contains four amino acid units, proline, 5-methoxyanthranilic acid, isoleucine and 3-aminoacrylic acid. its structure was determined on the basis of nmr, hr-ms and ms(n) spectral data analysis. the two unusual amino acid residues, 5-methoxyanthranilic acid and 3-aminoacrylic acid, were first foun ... | 2014 | 24942057 |
| new phenyl derivatives from endophytic fungus aspergillus flavipes ail8 derived of mangrove plant acanthus ilicifolius. | two new aromatic butyrolactones, flavipesins a (1) and b (2), two new natural products (3 and 4), and a known phenyl dioxolanone (5) were isolated from marine-derived endophytic fungus aspergillus flavipes. the structures of compounds 1-5 were elucidated by 1d- and 2d-nmr and ms analysis, the absolute configurations were assigned by optical rotation and cd data, and the stereochemistry of 1 was determined by x-ray crystallography analysis. 1 demonstrated lower mic values against staphylococcus a ... | 2014 | 24704337 |
| induction of pectinase hyper production by multistep mutagenesis using a fungal isolate--aspergillus flavipes. | aspergillus flavipes, a slow growing pectinase producing ascomycete, was isolated from soil identified and characterised in the previously done preliminary studies. optimisation studies revealed that citrus peel--groundnut oil cake [cg] production media is the best media for production of high levels of pectinase up to 39 u/ml using wild strain of a. flavipes. strain improvement of this isolated strain for enhancement of pectinase production using multistep mutagenesis procedure is the endeavour ... | 2014 | 26563068 |
| co-immobilization of pegylated aspergillus flavipes l-methioninase with glutamate dehydrogenase: a novel catalytically stable anticancer consortium. | aspergillus flavipes l-methioninase (afmetase) exhibits reliable pharmacokinetic properties and anticancer potency in vitro[10]. to maximize its therapeutic efficiency as protection against in vivo proteolysis, reduction of antigenicity and hyperammoniemia, the enzyme was pegylated and coupled with glutamate dehydrogenase (gdh). the highest degree of pegylation was measured at 40-50/1 molar ratio of peg to afmetase, with a lower mobility on sds-pge, compared to the native afmetase. the activity ... | 2014 | 24267569 |
| plant and fungal diversity in gut microbiota as revealed by molecular and culture investigations. | few studies describing eukaryotic communities in the human gut microbiota have been published. the objective of this study was to investigate comprehensively the repertoire of plant and fungal species in the gut microbiota of an obese patient. | 2013 | 23555039 |
| oxygen transfer coefficient and the kinetic parameters of exo-polygalacturonase production by aspergillus flavipes fp-500 in shake flasks and bioreactor. | to evaluate and compare the sensitivity of exo-pg production and kinetic parameters of aspergillus flavipes fp-500 to oxygen transfer condition in shake flasks and bioreactor. | 2012 | 25999036 |
| pharmacokinetics, immunogenicity and anticancer efficiency of aspergillus flavipes l-methioninase. | methionine starvation can powerfully modulate dna methylation, cell cycle transition, polyamines and antioxidant synthesis of tumor cells, in contrary to normal ones. aspergillus flavipesl-methioninase was previously characterized by our studies, displaying affordable biochemical properties comparing to pseudomonas putida enzyme (oncase). thus, the objective of current study was to evaluate the catalytic properties of af-metase in new zealand rabbits, exploring its antitumor efficacy. in vivo, a ... | 2012 | 22883554 |
| four butyrolactones and diverse bioactive secondary metabolites from terrestrial aspergillus flavipes mm2: isolation and structure determination. | the chemical constituents and biological activities of the terrestrial aspergillus flavipes mm2 isolated from egyptian rice hulls are reported. seven bioactive compounds were obtained, of which one sterol: ergosterol (1), four butyrolactones: butyrolactone i (2), aspulvinone h (3), butyrolactone-v (6) and 4,4'-diydroxypulvinone (7), along with 6-methylsalicylic acid (4) and the cyclopentenone analogue; terrien (5). structures of the isolated compounds were deduced by intensive studies of their 1 ... | 2012 | 22380482 |
| flavimycins a and b, dimeric 1,3-dihydroisobenzofurans with peptide deformylase inhibitory activity from aspergillus flavipes. | flavimycins a (1) and b (2), novel dimeric 1,3-dihydroisobenzofurans, were isolated as inhibitors of peptide deformylase from cultures of aspergillus flavipes. their chemical structures were established by nmr and ms data analysis. compounds 1 and 2 exist as epimeric mixtures at c-1 through fast hemiacetal-aldehyde tautomerism. compounds 1 and 2 inhibited staphylococcus aureus peptide deformylase with ic₅₀ values of 35.8 and 100.1 μm, respectively. consistent with their pdf inhibition, 1 showed ... | 2012 | 22329646 |
| constitutive and inducible pectinolytic enzymes from aspergillus flavipes fp-500 and their modulation by ph and carbon source. | growth and enzymes production by aspergillus flavipes fp-500 were evaluated on pectin, polygalacturonic acid, galacturonic acid, arabinose, rhamnose, xylose, glycerol and glucose at different initial ph values. we found that the strain produced exopectinases, endopectinases and pectin lyases. exopectinases and pectin lyase were found to be produced at basal levels as constitutive enzymes and their production was modulated by the available carbon source and ph of culture medium and stimulated by ... | 2009 | 24031315 |
| aspergilasines a-d: four merocytochalasans with new carbon skeletons from aspergillus flavipes qcs12. | aspergilasines a-d (1-4), four merocytochalasans with new skeletons, were isolated from the liquid culture broth of aspergillus flavipes qcs12. aspergilasine a (1) possesses a uniquely caged pentacyclo[7.2.0.1(4,11).0(2,7).0(5,10)]dodecane skeleton with an unexpected cyclobutane ring that formed by the fusion of two epicoccine moieties via two [3 + 2] cycloadditions. aspergilasines b-d (2-4) are key biosynthetic intermediates of epicochalasine b that fit well with our previous biosynthetic propo ... | 2017 | 28783364 |
| isolation and structure elucidation of a new compound from the fungus aspergillus flavipes pj03-11. | a new diphenyl ether 3-methylpentyl-2, 4-dichloroasterrate (2), along with a known diphenyl ether butyl 2, 4-dichloroasterrate (1) were isolated from the metabolites of a wetland fungus aspergillus flavipes. pj03-11. the structures of 1 and 2 were determined by extensive nmr and hr-esi-ms experiments. compounds 1 and 2 showed weak cytotoxic activity, but both of them showed no antimicrobial activity. | 2017 | 28494651 |