| [radial growth rate of fungal colonies in respect of their ecology]. | indices of the radial growth rate of colonies were determined for circinella circinans and mortierella ramanniana as well as for the complex of fungi in soil in the course of microbial succession. the maximum of growth (kr = 0.053 cm/hr) for c. circinans was observed at a high concentration of glucose (10%) in the medium, whereas for m. ramanniana the maximum (kr = 0.0054 cm/hr) was detected at a low glucose concentration (0.005%) in the medium. these data account for the position of c. circinan ... | 1978 | 713887 |
| enzyme-controlled reactions giving alkanols of use in the synthesis of biologically active molecules. | (+/-)-bicyclo[3.2.0]hept-2-en-6-one was reduced using a variety of fungi including yeasts. baker's yeast gave 6-exo-(1r,5s,6s)-bicyclo[3.2.0]hept-2-en-6-ol and 6-endo-(1s,5r,6s)-bicyclo[3.2.0]hept-2-en-6-ol while curvularia lunata and mortierella ramanniana gave only the 6-endo-alcohol and optically active bicycloheptenone. the optically active bicycloheptenols were used to prepare prostaglandin f2 alpha in the natural configuration. | 1985 | 3893940 |
| lysis of cell walls of mucor ramannianus möller by a streptomyces sp. | | 1968 | 5301324 |
| the production of -carotene in mortierella ramanniana var. ramanniana m29:the effect of changes in the environment upon growth and pigmentation. | | 1971 | 5316514 |
| the esterases of mortierella ramanniana in relation to taxonomy. | | 1968 | 5652098 |
| effect of antimicrobial agents on the milky disease bacteria bacillus popilliae and bacillus lentimorbus. | the effects of antibiotics, sulfonamides, and other antimicrobial agents on vegetative cultures of five strains of milky disease bacteria were compared with those on bacillus subtilis cohn emend. prazmowski, staphylococcus aureus rosenbach, sarcina lutea schroeter, escherichia coli (migula) castellani and chalmers, saccharomyces pastorianus hansen, and mucor ramannianus moel. similar numbers of viable cells of each organism were exposed to the test materials by use of an antibiotic-sensitivity d ... | 1965 | 5866030 |
| elaboration of vomitoxin and zearalenone by fusarium isolates and the biological activity of fusarium-produced toxins. | sixteen fusarium isolates belonging to f. graminearium schw. and f. culmorum (w.g. smith) sacc. produced vomitoxin and zearalenone on cracked corn at 28 degrees c. quantitation for vomitoxin was by gas-liquid chromatography. this toxin was produced in quantities of 5 to 236 microgram/g of fermented corn. vomitoxin showed weak antibiotic activity against penicillium digitatum sacc., mucor ramannianus möller, and saccharomyces bayanus sacc., but did not inhibit gram-positive, gram-negative, or aci ... | 1981 | 6459056 |
| photochemical interaction of dictamnine, a furoquinoline alkaloid, with fungal dna. | the furoquinoline alkaloid dictamnine has been shown to provoke lethal damage to filamentous fungi in near ultraviolet light. the phototoxicity was more pronounced against mucor hiemalis and mucor ramannianus than against fusarium graminearum and penicillium italicum. in vitro, labeled dictamnine was shown to form covalent monoadducts with purified dna from m. hiemalis in the presence of long-wave ultraviolet light. addition of [3h]dictamnine to cultures of the same organism showed photobinding ... | 1982 | 7104872 |
| [kinetics of the growth of colonies of mucor plumbeus and mortierella ramanniana fungi on agarized media with glucose]. | the growth kinetics of mucor plumbeus and mortierella ramanniana colonies was studied during cultivation of these two soil fungi on solid media with glucose as a limiting substrate. within the whole studied range of glucose concentrations (s), the radial growth rate of colonies (kr) can be satisfactorily described with the following equation that takes substrate inhibition into consideration: (formula: see text) the values of kinetic parameters for m. plumbeus and mort. ramanniana, respectively, ... | 1981 | 7219221 |
| characterization of the diacylglycerol acyltransferase activity in the lipid body fraction from an oleaginous fungus. | diacylglycerol acyltransferase (dgat) was examined as a key enzyme for triacylglycerol (tg) accumulation of an oleaginous fungus, mortierella ramanniana var. angulispora. subcellular fractionation of the fungus showed that dgat activity was highest in the lipid body fraction, which occupied 77% of the recovered dgat activity. dgat activity in the lipid body fraction was much higher than that in the membrane fraction in terms of both total activity and specific activity. similar results were obta ... | 1994 | 7706220 |
| halicylindramides a-c, antifungal and cytotoxic depsipeptides from the marine sponge halichondria cylindrata. | halicylindramides a-c (1-3) have been isolated from the japanese marine sponge halichondria cylindrata. they are tetradecapeptides with the n-terminus blocked by a formyl group and the c-terminus lactonized with a threonine residue. their total structures including absolute stereochemistry were determined by a combination of spectral and chemical methods. halicylindramides a-c were antifungal against mortierella ramanniana and cytotoxic against p388 murine leukemia cells. | 1995 | 7830276 |
| stereoselective epoxidation of 2,2-dimethyl-2h-1-benzopyran-6-carbonitrile. | the chiral intermediate (3s,4r)-trans-3,4-dihydro-3,4-dihydroxy-2,2-dimethyl- 2h-1-benzopyran-6-carbonitrile [(+)-trans diol 3] was made by the stereoselective microbial epoxidation of 2,2-dimethyl-2h-1-benzopyran-6-carbonitrile 1. this compound is a potential intermediate for the total synthesis of potassium-channel openers. several microbial cultures were found which catalyzed the transformation of 1 to the corresponding (3s,4s)-epoxide 2 and (+)-trans diol 3. the two best cultures, corynebact ... | 1994 | 8000876 |
| microbial reduction of 1-(4-fluorophenyl)-4-[4-(5-fluoro-2-pyrimidinyl)-1- piperazinyl]butan-1-one. | among various micro-organisms screened for the stereoselective reduction of 4-chloro-1-(4-fluorophenyl)butan-1-one (1), hansenula polymorpha [american type culture collection (a.t.c.c.) 26012 and 86014], nocardia salmonicolor [squibb culture (s.c.) 6370], arthobacter simplex (a.t.c.c. 6949), mycobacterium vaccae (a.t.c.c. 29678), candida boidinii (a.t.c.c. 13821) and saccharomyces cerevisiae (a.t.c.c. 13792) reduced compound 1 to the corresponding (r)-(+)-alcohol (2). in contrast, lactobacillus ... | 1993 | 8166884 |
| activation of detergent-solubilized diacylglycerol acyltransferase by anionic phospholipids. | diacylglycerol acyltransferase (dgat), which catalyzes the final step in triacylglycerol (tg) biosynthesis, is crucial for lipid accumulation and formation of lipid bodies in an oleaginous fungus, mortierella ramanniana var. angulispora. since solubilization of dgat in the lipid body fraction from this fungus with 0.5% triton x-100 gave very low recovery of the activity, some activation factors for solubilized dgat activity were investigated. addition of phospholipids, especially anionic phospho ... | 1996 | 8830048 |
| oleate 6-hydroxylase activity in the membrane fraction from an oleaginous fungus, mortierella ramanniana var. angulispora. | conversion of [1-14c]oleoyl-coa (oa-coa) in subcellular fractions of an oleaginous fungus, mortierella ramanniana var. angulispora (ifo 8187) was investigated. the membrane fraction actively catalyzed the transformation of oa-coa into one metabolite, which was identified as 6-hydroxy oleic acid (6-hooa) by gc-ms analysis. the enzyme activity to form 6-hooa was dependent on oa-coa concentrations and increased linearly with incubation time and protein concentrations under appropriate experimental ... | 1996 | 8944750 |
| halicylindramides d and e, antifungal peptides from the marine sponge halichondria cylindrata. | halicylindramides d (1) and e (2) have been isolated from the marine sponge halichondria cylindrata. halicylindramide d is a tridecapeptide with the n-terminus blocked by a formyl group and the c-terminus lactonized with a threonine residue, while halicylindramide e is a truncated linear peptide with a c-terminal amide. their structures, including absolute stereochemistry, were determined by a combination of spectral and chemical methods. halicylindramide d was antifungal against mortierella ram ... | 1996 | 8991950 |
| purification and characterization of diacylglycerol acyltransferase from the lipid body fraction of an oleaginous fungus. | diacylglycerol acyltransferase (dgat) [ec 2.3.1.20] was purified to apparent homogeneity from the lipid body fraction of an oleaginous fungus, mortierella ramanniana var. angulispora. the enzyme was solubilized from the lipid body fraction with 0.1% triton x-100, and purified by subsequent column chromatography on yellow 86 agarose, superdex-200, heparin-sepharose, second superdex-200, and second yellow 86 agarose. the enzyme activity was finally enriched 4,802-fold over that of the starting 1,5 ... | 1997 | 9354384 |
| characterization of triacylglycerol biosynthesis in subcellular fractions of an oleaginous fungus, mortierella ramanniana var. angulispora. | triacylglycerol (tg) biosynthetic enzymes were characterized in subcellular fractions of an oleaginous fungus, mortierella ramanniana var. angulispora. when the membrane or lipid body fraction of this fungus was incubated with [14c]oleoyl-coa without adding exogenous acyl acceptors, radioactivity was incorporated predominantly into tg, indicating that diacylglycerol acyltransferase (dgat) used endogenous diacylglycerol to incorporate [14c]oleoyl-coa into tg. adding glycerol 3-phosphate or lysoph ... | 1998 | 9714775 |
| new antifungal and cytotoxic steroidal saponins from the bulbs of an elephant garlic mutant. | saponins in bulbs of a mutant of elephant garlic were investigated, and three new steroidal saponins named yayoisaponins a-c were obtained together with the known dioscin and aginoside. their structures, including the relative stereochemistry, were elucidated by spectral data interpretation, while the absolute stereochemistry of the sugar moieties was assigned on the basis of a chiral gas chromatographic analysis of the acid hydrolysates. yayoisaponins a-c and aginoside exhibited not only in vit ... | 1998 | 9836426 |
| biodegradation of fluoranthene by soil fungi. | a selection of 39 strains of micromycetes known as good degraders of polychlorinated aromatic compounds, mostly isolated from soil and belonging to various taxonomic groups, have been investigated for fluoranthene degradation. toxicity assays, first evaluated on solid medium mea, have not shown any toxicity of fluoranthene (1-100 mg.l-1) towards fungi. whereas, consumption assays on a solid synthetic medium showed a toxicity at 100 mg.l-1. the degradation of fluoranthene (10 mg.l-1) was then inv ... | 1999 | 10230046 |
| lipid bodies and lipid body formation in an oleaginous fungus, mortierella ramanniana var. angulispora. | mortierella ramanniana var. angulispora accumulates triacylglycerol (tg) in lipid bodies. studies on lipid transport into lipid bodies are essential for elucidating mechanisms of lipid body formation. we used fluorescent dyes and fluorescent lipid analogs to visualize lipid body formation with a confocal laser scanning microscope. different sizes of lipid bodies were stained by nile red, a lipid body marker - one with a diameter of about 1 micrometer and the other with a diameter of about 2-3 mi ... | 1999 | 10320801 |
| regioselective transformation of ciprofloxacin to n-acetylciprofloxacin by the fungus mucor ramannianus. | a strain of the saprobic fungus mucor ramannianus, isolated from a forest, was used to demonstrate the potential for ciprofloxacin biotransformation by zygomycetes in the environment. the fungus carried out the regioselective n-acetylation of ciprofloxacin to a single product, which was purified from culture extracts by high-performance liquid chromatography. the metabolite was identified by mass and nuclear magnetic resonance spectrometry as 1-cyclopropyl-6-fluoro-1,4-dihydro-4-oxo-7-(4-acetyl- ... | 1999 | 10436931 |
| phylogeny and pcr identification of clinically important zygomycetes based on nuclear ribosomal-dna sequence data. | a molecular database for all clinically important zygomycetes was constructed from nucleotide sequences from the nuclear small-subunit (18s) ribosomal dna and domains d1 and d2 of the nuclear large-subunit (28s) ribosomal dna. parsimony analysis of the aligned 18s and 28s dna sequences was used to investigate phylogenetic relationships among 42 isolates representing species of zygomycetes reported to cause infections in humans and other animals, together with commonly cultured contaminants, with ... | 1999 | 10565914 |
| microbial transformation of papaveraldine. | preparative-scale fermentation of papaveraldine (1), the known benzylisoquinoline alkaloid, with mucor ramannianus 1839 (sih) has resulted in a stereoselective reduction of the ketone group and the isolation of s-papaverinol (2) and s-papaverinol n-oxide (3). the structure elucidation of both metabolites was based primarily on 1d-, 2d-nmr analyses and chemical transformations. the absolute configuration of 2 was determined using horeau's method of asymmetric esterification. these metabolism resu ... | 2000 | 10746880 |
| microbiological transformation of enrofloxacin by the fungus mucor ramannianus. | enrofloxacin metabolism by mucor ramannianus was investigated as a model for the biotransformation of veterinary fluoroquinolones. cultures grown in sucrose-peptone broth were dosed with enrofloxacin. after 21 days, 22% of the enrofloxacin remained. three metabolites were identified: enrofloxacin n-oxide (62% of the total absorbance), n-acetylciprofloxacin (8.0%), and desethylene-enrofloxacin (3.5%). | 2000 | 10831454 |
| intracellular transport of phosphatidic acid and phosphatidylcholine into lipid bodies in an oleaginous fungus, mortierella ramanniana var. angulispora. | exogenous fluorescent phosphatidic acid (pa) and phosphatidylcholine (pc) were transported into lipid bodies in an oleaginous fungus, mortierella ramanniana var. angulispora [kamisaka et al. (1999) biochim. biophys. acta 1438, 185-198]. we further investigated the processes of fluorescent pa and pc transport into lipid bodies in this fungus by changing culture conditions. lowering incubation temperature decreased lipid body labeling by 1-palmitoyl, 2-[5-(5,7-dimethyl boron dipyrromethene difluor ... | 2001 | 11134953 |
| intracellular transport of phosphatidic acid and phosphatidylcholine into lipid bodies: use of fluorescent lipids to study lipid-body formation in an oleaginous fungus. | fluorescent phosphatidic acid and phosphatidylcholine were used to characterize lipid-transport pathways into lipid bodies in an oleaginous fungus, mortierella ramanniana var. angulispora. several characteristics of the lipid transport such as temperature dependence and atp dependence were evaluated. the transport depicted by these fluorescent lipids was consistent with metabolism of radiolabelled lipids, indicating that fluorescent lipids are useful to study lipid-body formation in this fungus. ... | 2000 | 11171185 |
| microbial transformation of hypoestenone. | preparative-scale fermentation of hypoestenone (1) with mucor ramannianus (atcc 9628) has resulted in the isolation of 8(9)alpha-epoxyhypoestenone (2) and the new metabolites 8(9)alpha-epoxy-12,13-anhydrohypoestenone (3), 17-hydroxyhypoestenone (4), 13alpha,18-dihydroxyhypoestenone (5), and 13beta,18-dihydroxyhypoestenone (6). structure elucidation of these metabolites was based primarily on 1d and 2d nmr analyses. | 2001 | 11277761 |
| purification and characterization of thiol-reagent-sensitive glycerol-3-phosphate acyltransferase from the membrane fraction of an oleaginous fungus. | glycerol-3-phosphate acyltransferase (gpat), responsible for the first committed, rate-limiting, step of glycerolipid synthesis, was purified to homogeneity from the membrane fraction of an oleaginous fungus, mortierella ramanniana var. angulispora. the enzyme was solubilized from the membrane fraction by pretreatment with 0.05% triton x-100 and treatment of the resulting pellet with 0.3% triton x-100. the enzyme was subsequently purified by column chromatography on heparin-sepharose, yellow 86 ... | 2001 | 11284717 |
| microbial epoxidation of the tricyclic sesquiterpene presilphiperfolane angelate ester. | microbial transformation studies on 2beta-angeloyloxy-5beta,8beta-dihydroxypresilphiperfolane have revealed that it was metabolized by a number of microorganisms. using a standard two-stage fermentation technique, mucor ramannianus (atcc 9628) produced three metabolites. one of them was characterized as the novel metabolite (2'r,3'r)-(+)-2beta-(2',3'-epoxyangeloyloxy)-5beta,8beta-dihydroxypresilphiperfolane on the basis of spectral data. the absolute configuration at both oxirane carbons was con ... | 2001 | 11371012 |
| metabolism of the veterinary fluoroquinolone sarafloxacin by the fungus mucor ramannianus. | to investigate the microbial biotransformation of veterinary fluoroquinolones, mucor ramannianus was grown in sucrose/peptone broth with sarafloxacin for 18 days. cultures were extracted with ethyl acetate and extracts were analyzed by liquid chromatography. the two metabolites (26% and 15% of the a280, respectively) were identified by mass and 1h nuclear magnetic resonance spectra as n-acetylsarafloxacin and desethylene-n-acetylsarafloxacin. the biological formation of desethylene-n-acetylsaraf ... | 2001 | 11420653 |
| dgat2 is a new diacylglycerol acyltransferase gene family: purification, cloning, and expression in insect cells of two polypeptides from mortierella ramanniana with diacylglycerol acyltransferase activity. | acyl coa:diacylgycerol acyltransferase (ec; dgat) catalyzes the final step in the production of triacylglycerol. two polypeptides, which co-purified with dgat activity, were isolated from the lipid bodies of the oleaginous fungus mortierella ramanniana with a procedure consisting of dye affinity, hydroxyapatite affinity, and heparin chromatography. the two enzymes had molecular masses of 36 and 36.5 kda, as estimated by gel electrophoresis, and showed a broad activity maximum between ph 6 and 8. ... | 2001 | 11481333 |
| the dga1 gene determines a second triglyceride synthetic pathway in yeast. | diacylglycerol esterification provides an excellent target for the pharmacological reduction of triglyceride accumulation in several human disease states. we have used saccharomyces cerevisiae as a model system to study this critical component of triglyceride synthesis. recent studies of an oleaginous fungus, mortierella ramanniana, identified a new family of enzymes with in vitro acyl-coa:diacylglycerol acyltransferase activity. we show here that dga1, the sole member of this gene family in yea ... | 2002 | 11751875 |
| biosynthesis of triacylglycerol molecular species in an oleaginous fungus, mortierella ramanniana var. angulispora. | the incorporation of radiolabeled lipid precursors into triacylglycerol (tg) molecular species in mortierella ramanniana var. angulispora, an oleaginous fungus, was studied to determine the biosynthetic pathways for tg molecular species. radiolabeled tg molecular species were separated and quantified by reverse-phase high performance liquid chromatography with a radioisotope detector. the major tg molecular species labeled by [1-(14)c]oleic acid at 30 degrees c were oop, ooo, and opp (tg molecul ... | 2002 | 12097168 |
| ecology of mucor ramannianus moller. | | 1959 | 13657118 |
| appearance of smaller lipid bodies and protein kinase activation in the lipid body fraction are induced by an increase in the nitrogen source in the mortierella fungus. | we studied the regulation of lipid body biogenesis in the oleaginous fungus mortierella ramanniana var. angulispora by investigating culture conditions to modulate lipid body size, which we found was affected by the carbon-to-nitrogen ratio (c/n ratio) in the culture medium. increasing the nitrogen source or decreasing the c/n ratio from 38 to 9 induced the appearance of lipid bodies with diameters less than 2-3 micro m, which are usually found at a c/n ratio of 38 in this fungus. to determine f ... | 2004 | 15047730 |
| inhibition of ptp1b by metabolites from micromucor ramannianus var. angulisporus crm000232. | | 2004 | 15515890 |
| biodegradation of the insecticide n,n-diethyl-m-toluamide by fungi: identification and toxicity of metabolites. | fungi (cunninghamella elegans atcc 9245, mucor ramannianus r-56, aspergillus niger vkmf-1119, and phanerochaete chrysosporium bkmf-1767) were tested to elucidate the biologic fate of the topical insect repellent n,n-diethyl-m-toluamide (deet). the elution profile obtained from analysis by high-pressure liquid chromatography equipped with a reverse-phase c-18 column, showed that three peaks occurred after incubation of c. elegans, with which 1 mm deet was combined as a final concentration. the pe ... | 2005 | 15750774 |
| fungal biotransformation of benzo[f]quinoline, benzo[h]quinoline, and phenanthridine. | cultures of umbelopsis ramanniana (=mucor ramannianus) were grown in fluid sabouraud medium for 3 days, dosed with 0.23 mm benzo[f]quinoline, benzo[h]quinoline, or phenanthridine (benzo[c]quinoline), and incubated for another 18 days. cultures were extracted and metabolites (66-75% of the uv absorbance) were separated by high-performance liquid chromatography. they were identified by mass spectrometry and nuclear magnetic resonance spectroscopy. benzo[f]quinoline was metabolized to benzo[f]quino ... | 2004 | 15856220 |
| oribatid mite (acari, oribatida) feeding on ectomycorrhizal fungi. | the coexistence of a large number of soil animals without extensive niche differentiation is one of the great riddles in soil biology. the main aim of this study was to explore the importance of partitioning of food resources for the high diversity of micro-arthropods in soil. in addition, we investigated if ectomycorrhizal fungi are preferentially consumed compared to saprotrophic fungi. until today, ectomycorrhizal fungi have never been tested as potential food resource for oribatid mites. we ... | 2005 | 16133254 |
| antibiotics against plant disease: viii. screening for nonpolyenic antifungal antibiotics produced by streptomycetes. | in a survey of streptomyces species, methods were designed and followed that would specifically select strains capable of producing heat-stable, nonpolyenic, antifungal antibiotics. of 500 strains grown in shaken flasks, 240 of the culture liquors contained active factors as demonstrated by paper-disc assay against mucor ramannianus. culture filtrates and mycelial extracts of the active strains were examined by ultraviolet spectrophotometry; 166 were nonpolyenic as determined by absorption spect ... | 1964 | 16349649 |
| fungal biodegradation of carbofuran and carbofuran phenol by the fungus mucor ramannianus: identification of metabolites. | the fungus mucor ramannianus was tested to elucidate the biological fate of a class of n-methyl carbamate pesticide carbofuran and its hydrolysed form carbofuran phenol. the elution profile obtained from analysis by high-pressure liquid chromatography equipped with a reverse-phase c-18 column showed that two peaks occurred after incubation of m. ramannianus with which 1 mm carbofuran was combined as a final concentration. in culture of m. ramannianus with 1 mm carbofuran phenol, it produced two ... | 2007 | 17305136 |
| isolation and partial characterization of antimicrobial compounds from a new strain nonomuraea sp. nm94. | an actinomycete strain nm94 was isolated from a saharan soil sample by a dilution agar plating method using chitin-vitamins b medium supplemented with penicillin. the strain presented the morphological and chemical characteristics of the genus nonomuraea. on the basis of 16s rdna analysis and physiological tests, this isolate was found to be quite different from the known species of nonomuraea and might be new. the strain nm94 secreted several antibiotics on yeast extract malt extract glucose me ... | 2007 | 17318487 |
| biocatalysis of the anticancer sipholane triterpenoids. | the red sea sponge callyspongia (= siphonochalina) siphonella is a rich source of sipholane triterpenoids. biocatalysis of the major sipholanes, sipholenol a (1) and sipholenone a (2), respectively, by mucor ramannianus atcc 9628 and cunninghamella elegans atcc 7929 afforded four new metabolites 3 - 6 along with sipholenol g and 28-hydroxysipholenol a. major sipholanes along with their biocatalytic products were investigated for their antiproliferative activity against the highly malignant +sa m ... | 2007 | 17486535 |
| microfungi associated with abies needles and betula leaf litter in a subalpine coniferous forest. | we investigated microfungal assemblages on leaf litter within a subalpine forest in central japan and their variation with season, litter depth, and litter species. microfungal assemblages were compared for abies needles and betula leaf litter collected from litter and fermentation layers of the forest floor during the growing season in spring, summer, and autumn. a total of 35 and 42 species were isolated from abies needles and betula leaf litter, respectively. the observed variation in microfu ... | 2007 | 17496944 |
| [sporangiospore lipids of the mycelial fungus mucor ramannianus incapable of dimorphic growth]. | members of the species mucor ramannianus are believed to be monomorphic. they grow only as a mycelium and are not capable of growth as budding cells, i.e., of dimorphic growth. in our study, we investigated the lipid composition of m. ramannianus sporangiospores, which retained the capability of initiating mycelial growth in the course of long-term cultivation of the spore-forming mycelium. it was demonstrated that sporangiospores contained high concentrations of triacylglycerides (tag) in their ... | 2007 | 17583213 |
| plant community influences on soil microfungal assemblages in boreal mixed-wood forests. | we studied the relationships between assemblages of soil microfungi and plant communities in the southern boreal mixed-wood forests of quebec. sampling took place in 18,100 m2 plots from an existing research site. plots were separated into three categories based on dominant overstory tree species: (i) trembling aspen, (ii) white birch and (iii) a mixture of white spruce and balsam fir. within each plot a 1 m2 subplot was established in which the understory herbaceous layer was surveyed and soil ... | 2007 | 17883027 |
| semisynthetic and biotransformation studies of (1s,2e,4s,6r,7e,11e)-2,7,11-cembratriene-4,6-diol. | tobacco-derived (1 s,2 e,4 s,6 r,7 e,11 e)-2,7,11-cembratriene-4,6-diol (1) and (1 s,2 e,4 r,6 r,7 e,11 e)-2,7,11-cembratriene-4,6-diol (2) were first shown to display potential antitumor-promoting activity in the mid-1980s. however, very little is currently understood regarding the structural activity relationships of tobacco cembranoids. the aim of this present study was to explore antiproliferative activity of various derivatives of (1 s,2 e,4 s,6 r,7 e,11 e)-2,7,11-cembratriene-4,6-diol (1) ... | 2008 | 18177013 |
| identification of fungal metabolites of anticonvulsant drug carbamazepine. | carbamazepine, which has been used in the treatments of epilepsy, is often found in the environment. although metabolism of carbamazepine by humans and rats has been characterized, the environmental fate of carbamazepine has not been studied. in this study, two model fungi cunninghamella elegans atcc 9245 and umbelopsis ramanniana r-56, which have previously shown diverse metabolic activities, were tested for metabolism of carbamazepine. both fungi produced three metabolites each (c1-c3 and m1-m ... | 2008 | 18414846 |
| expression of umbelopsis ramanniana dgat2a in seed increases oil in soybean. | oilseeds are the main source of lipids used in both food and biofuels. the growing demand for vegetable oil has focused research toward increasing the amount of this valuable component in oilseed crops. globally, soybean (glycine max) is one of the most important oilseed crops grown, contributing about 30% of the vegetable oil used for food, feed, and industrial applications. breeding efforts in soy have shown that multiple loci contribute to the final content of oil and protein stored in seeds. ... | 2008 | 18633120 |
| microbial metabolism of biologically active secondary metabolites from nerium oleander l. | ursolic acid (1) and kaempferol (3) are two major constituents of the mediterranean plant nerium oleander l. microbial metabolism of (1) with aspergillus flavus (atcc 9170) resulted in the formation of 3-oxo-ursolic acid derivative, ursonic acid (2). on the other hand, cunninghamella blakesleeana (atcc 8688a) was able to convert (3) into kaempferol 3-o-beta-d-glucopyranoside (4) as well as the new natural product kaempferol 4'-sulfate (5). incubation of kaempferol with mucor ramannianus (atcc 96 ... | 2008 | 18758096 |
| microbial metabolism. part 10: metabolites of 7,8-dimethoxyflavone and 5-methoxyflavone. | microbial transformation of 7,8-dimethoxyflavone (1) by mucor ramannianus produced five metabolites: 7,8-dimethoxy-4'-hydroxyflavone (2), 3',4'-dihydroxy-7,8-dimethoxyflavone (3), 7,3'-dihydroxy-8-methoxyflavone (4), 7,4'-dihydroxy-8-methoxyflavone (5) and 8-methoxy-7,3',4'-trihydroxyflavone (6). it was, however, completely converted to a single metabolite, 7-hydroxy-8-methoxyflavone (7) by aspergillus flavus. 5-methoxyflavone (8), when fermented with beauveria bassiana, gave a single product, 5 ... | 2009 | 19731142 |
| a molecular model for diacylglycerol acyltransferase from mortierella ramanniana var. angulispora. | acyl coa diacylglycerol acyltransferase (dgat, ec 2.3.120) is recognized as a key player of cellular diacylglycerol metabolism. it catalyzes the terminal, yet the committed step in triacylglycerol synthesis using diacylglycerol and fatty acyl coa as substrates. the protein sequence of diacylglycerol acyltransferse (dgat) type 2b in moretierella ramanniana var. angulispora (protein_id = aak84180.1) was retrieved from genbank. however, a structure is not yet available for this sequence. the 3d str ... | 2009 | 19759814 |
| microbial transformation of podocarpic acid and evaluation of transformation products for antioxidant activity. | podocarpic acid was metabolized by mucor ramannianus atcc 9628, and beauveria bassiana atcc 7159 to afford two new metabolites, 2 alpha-hydroxy podocarpic acid and 11-hydroxy podocarpic acid, along with the known metabolite 13-hydroxy podocarbic acid. the identity of these metabolites was verified using different spectral methods. the antioxidant activity of the metabolites was tested, and comparable results to those observed for ascorbic acid were obtained. | 2010 | 20072957 |
| microbial metabolism of cannflavin a and b isolated from cannabis sativa. | microbial metabolism of cannflavin a (1) and b (2), two biologically active flavonoids isolated from cannabis sativa l., produced five metabolites (3-7). incubation of 1 and 2 with mucor ramannianus (atcc 9628) and beauveria bassiana (atcc 13144), respectively, yielded 6''s,7''-dihydroxycannflavin a (3), 6''s,7''-dihydroxycannflavin a 7-sulfate (4) and 6''s,7''-dihydroxycannflavin a 4'-o-alpha-l-rhamnopyranoside (5), and cannflavin b 7-o-beta-d-4'''-o-methylglucopyranoside (6) and cannflavin b 7 ... | 2010 | 20223485 |
| bioactive natural, biocatalytic, and semisynthetic tobacco cembranoids. | the two major nicotiana tabacum tobacco cembranoids, (1 s,2 e,4 r,6 r,7 e,11 e)-2,7,11-cembratriene-4,6-diol (1) and its c-4 epimer, exhibit a wide range of interesting biological activities. although the tumorigenesis inhibition activity of tobacco cembranoids have been known since the mid 1980's, only a limited number of investigations have targeted their optimization and structure-activity relationship. this study reports the isolation of the new (1 s,2 e,4 s,6 e,8 s,11 e)-2,6,11-cembratriene ... | 2010 | 21049399 |
| expression of fungal diacylglycerol acyltransferase2 genes to increase kernel oil in maize. | maize (zea mays) oil has high value but is only about 4% of the grain by weight. to increase kernel oil content, fungal diacylglycerol acyltransferase2 (dgat2) genes from umbelopsis (formerly mortierella) ramanniana and neurospora crassa were introduced into maize using an embryo-enhanced promoter. the protein encoded by the n. crassa gene was longer than that of u. ramanniana. it included 353 amino acids that aligned to the u. ramanniana dgat2a protein and a 243-amino acid sequence at the amino ... | 2011 | 21245192 |
| microbial metabolism. part 12. | fermentation of 4'-hydroxyflavanone (1) with fungal cultures, beauveria bassiana (atcc 13144 and atcc 7159) yielded 6,3',4'-trihydroxyflavanone (2), 3',4'-dihydroxyflavanone 6-o-β-d-4-methoxyglucopyranoside (3), 4'-hydroxyflavanone 3'-sulfate (4), 6,4'-dihydroxyflavanone 3'-sulfate (5) and 4'-hydroxyflavanone 6-o-β-d-4-methoxyglucopyranoside (7). b. bassiana (atcc 13144) and b. bassiana (atcc 7159) in addition, gave one more metabolite each, namely, flavanone 4'-o-β-d-4-methoxyglucopyranoside (6 ... | 2011 | 21628902 |
| Lipids Containing Polyunsaturated Fatty Acids Synthesized by Zygomycetes Grown on Glycerol. | Several strains of Zygomycetes cultivated on glycerol produced mycelia rich in lipids containing higher amounts of neutral lipids (NL) than glycolipids plus sphingolipids and phospholipids (P), while biosynthesis of P in Mortierella ramanniana, Mucor sp., and Cunninghamella echinulata occurred though NL accumulation process was in progress. Polyunsaturated fatty acids (PUFA) concentration gradually decreased in all lipid fractions of M. ramanniana during growth. In contrast, in C. echinulata con ... | 2011 | 22057906 |
| microbial metabolism. part 13. metabolites of hesperetin. | the fungal culture, mucor ramannianus (atcc 2628) transformed hesperitin (1) to four metabolites: 4'-methoxy-5,7,8,3'-tetrahydroxyflavanone (8-hydroxyhesperetin) (2), 5,7,3',4'-tetrahydroxyflavanone (eriodictyol) (3), 4'-methoxy-5,3'-dihydroxyflavanone 7-sulfate (hesperetin 7-sulfate) (4) and 5,7,3'-trihydroxyflavanone 4'-o-α-quinovopyranoside (eriodictyol 4'-o-α-quinovopyranoside) (5). the structures were established by spectroscopic methods. | 2011 | 21873058 |
| hydroxylation of 1,8-cineole by mucor ramannianus and aspergillus niger. | the monoterpenoid 1,8-cineole is obtained from the leaves of eucalyptus globulus and it has important biological activities. it is a cheap natural substrate because it is a by-product of the eucalyptus cultivation for wood and pulp production. in this study, it was evaluated the potential of three filamentous fungi in the biotransformation of 1,8-cineole. the study was divided in two steps: first, reactions were carried out with 1,8-cineole at 1 g/l for 24 h; afterwards, reactions were carried o ... | 2015 | 26221115 |
| season's variation impact on citrus aurantium leaves essential oil: chemical composition and biological activities. | citrus aurantium leaves' essential oils (eos) were evaluated for chemical composition and antioxidant and antibacterial activities. the vegetable material, taken 5 times during the year, has undergone the hydrodistillation to prepare eo. chemical characterization by gas chromatography/mass spectrometry and gc/flame ionization detection allowed the identification of 46 compounds, and a notable quantitative and qualitative differences between the different petitgrain samples according to the harve ... | 2012 | 22897411 |
| eleven microbial metabolites of 6-hydroxyflavanone. | 6-hydroxyflavanone (1) when fermented with fungal culture cunninghamella blakesleeana (atcc 8688a) yielded flavanone 6-o-β-d-glucopyranoside (2), flavanone 6-sulfate (3), and 6-hydroxyflavanone 7-sulfate (4). aspergillus alliaceus (atcc 10060) also transformed 1 to metabolite 3 as well as 4'-hydroxyflavanone 6-sulfate (5) and 6,4'-dihydroxyflavanone (6). beauveria bassiana (atcc 7159) metabolized 1 to 6 and flavanone 6-o-β-d-4-o-methyglucopyranoside (7). mucor ramannianus (atcc 9628) transformed ... | 2015 | 26235165 |
| bioconversion of 7-hydroxyflavanone: isolation, characterization and bioactivity evaluation of twenty-one phase i and phase ii microbial metabolites. | microbial metabolism of 7-hydroxyflavanone (1) with fungal culture cunninghamella blakesleeana (atcc 8688a), yielded flavanone 7-sulfate (2), 7,4'-dihydroxyflavanone (3), 6,7-dihydroxyflavanone (4), 6-hydroxyflavanone 7-sulfate (5), and 7-hydroxyflavanone 6-sulfate (6). mortierella zonata (atcc 13309) also transformed 1 to metabolites 2 and 3 as well as 4'-hydroxyflavanone 7-sulfate (7), flavan-4-cis-ol 7-sulfate (8), 2',4'-dihydroxychalcone (9), 7,8-dihydroxyflavanone (10), 8-hydroxyflavanone 7 ... | 2012 | 22976322 |
| the olive mill wastewater as substrate for single cell oil production by zygomycetes. | the conversion of olive mill wastewater (omw) into high added value lipids containing polyunsaturated fatty acids (pufa), in parallel with a significant phenolic removal by selected strains of zygomycetes, is reported here for the first time. the growth of mortierella isabellina, mortierella ramanniana, cunninghamella echinulata, mucor sp., thamnidium elegans and zygorhynchus moelleri on solidified media was not significantly affected by the presence of omw used in the growth medium up to 50% (v ... | 2014 | 24316440 |
| regio- and stereo-selective hydroxylations of ingenane diterpenoids by mortierella ramanniana and gibberella fujikuroi. | the regio- and stereo-selective hydroxylations of two ingenane diterpenoids, 20-deoxyingenol (1) and 13-oxyingenol dodecanoat (2), by the filamentous fungi mortierella ramanniana and gibberella fujikuroi were investigated in the present study. four undescribed metabolites (3-6) of substrate 1 and two undescribed metabolites (7 and 8) of substrate 2 were isolated. all the metabolites were identified as hydroxylated ingenane derivatives by extensive nmr and hr-esi-ms data analyses. all the biotran ... | 2016 | 28262122 |