Publications

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induced segregation in interspecific hybrids of aspergillus nidulans and aspergillus rugulosus obtained by protoplast fusion.interspecific hybrids produced by polyethylene glycol induced fusion of protoplasts from auxotrophic mutants of aspergillus nidulans and aspergillus rugulosus were grown in the presence of the recombinogens benomyl and chloral hydrate to stimulate segregation. the a. nidulans parental strains used had a known genetic marker in each linkage group. hybrids grown on complete medium containing benomyl yielded more segregants. analysis of the segregants showed that the distribution of a. nidulans lin ...1979372762
new species of aspergillus producing sterigmatocystin.a number of species belonging to the genus aspergillus were evaluated for their toxicity to ducklings and the ability to produce sterigmatocystin. three new species capable of producing sterigmatocystin were found, namely, aspergillus aurantio-brunneus, aspergillus quadrilineatus, and aspergillus ustus. all three were toxic to ducklings. the production of sterigmatocystin by aspergillus rugulosus was confirmed, and the toxicity of aspergillus stellatus and aspergillus multicolor is described.1977406838
characterization of interspecific hybrids within the aspergillus nidulans group by isoenzyme analysis.a comparison of interspecific hybrids within the aspergillus nidulans species group was made by isoenzyme analysis. the gel electrophoretic patterns of the parental species were distinct for most of the enzymes tested. the hybrids were distinguishable from their parents by isoenzyme patterns. the appearance of novel bands in all the interspecific hybrids indicated that nuclear fusion could have occurred. in most hybrids the appearance of the parental bands showed nonpreferential, partial chromos ...19911878817
a new antifungal antibiotic (x-73) from aspergillus rugulosus thom and raper. 19704248104
the biosynthesis of phenols. xxiv. arugosin c, a metabolite of a mutant strain of aspergillus rugulosus. 19734796651
the biosynthesis of phenols. xxi. the molecular structure of arugosin, a metabolite of a wild-type strain of aspergillus rugulosus. 19705463825
a uv-sensitive mutant of aspergillus rugulosus unrelated to intergenic crossing over. 19685654999
the biosynthesis of phenols. ix. asperugin, a metabolic product of aspergillus rugulosus. 19655891945
the isolation of ultraviolet sensitive mutants from aspergillus rugulosus. 19676027880
aspects of genetic interaction in hybrids of aspergillus nidulans and aspergillus rugulosus obtained by protoplast fusion.hybrids were produced by protoplast fusion between strains of aspergillus rugulosus and mitotic master strains of aspergillus nidulans with a genetic marker on each linkage group. analysis of segregants induced by growth on benomyl revealed recombination between every pair of unlinked markers. parental combinations of markers were often recovered at significantly higher frequencies than expected. this aberrant segregation was not correlated with any particular pair of linkage-groups and was attr ...19836363618
a new sterigmatocystin-producing emericella variant from agricultural desert soils.an unusual, sterigmatocystin-producing taxon with characteristics of both emericella nidulans (anamorph aspergillus nidulans) and emericella rugulosa (anamorph aspergillus rugulovalvus, formerly a. rugulosus) was isolated repeatedly during a mycofloral survey of desert cotton field soils where aflatoxin is a chronic problem. members of this taxon had ascospores with smooth convex walls like e. nidulans but grew slowly like e. rugulosa; moreover, they were similar to an industrial echinocandin b- ...200111403392
the relationship of a phenolic metabolite and a morphological character of aspergillus rugulosus. 196414188774
the effects of supplementation and plating densities on apparently aberrant meiotic and mitotic segregation in aspergillus rugulosus. 196414239773
screening of aspergillus species for occurrence of lectins and their characterization.ten species of aspergillus were screened for occurrence of lectins. each of the species was investigated for the occurrence of extracellular, surface-bound and intracellular lectin activities. as many as four species namely, aspergillus niger, aspergillus versicolor, aspergillus rugulosus and aspergillus nidulans, were found to possess intracellular lectin activities, while none of the species showed extracellular or surface-bound lectin activities. each of the lectin was characterized with resp ...200818383222
prenylxanthones and a bicyclo[3.3.1]nona-2,6-diene derivative from the fungus emericella rugulosa.five new prenylxanthones, ruguloxanthones a-c (1-3), 14-methoxytajixanthone (4), and tajixanthone ethanoate (5), a new bicyclo[3.3.1]nona-2,6-diene derivative, rugulosone (6), and seven known compounds, shamixanthone, tajixanthone, 14-methoxytajixanthone-25-acetate, tajixanthone hydrate, tajixanthone methanoate, isoemericellin, and ergosterol, were isolated from the fungus emericella rugulosa. the structures of 1-6 were established using spectroscopic techniques. compound 6 exhibited antimalaria ...200919627125
Polyphasic characterization of "Aspergillus nidulans var. roseus" ATCC 58397.Polyphasic characterization of the echinocandin B producer Aspergillus nidulans var. roseus ATCC 58397 strain was carried out to elucidate its taxonomical status. According to its carbon source utilization and secondary metabolite spectrum as well as the partial ß-tubulin, calmodulin, and ?-actin gene sequences, A. nidulans var. roseus belongs to the Emericella rugulosa species. Auxotroph mutants of A. nidulans var. roseus ATCC 58397 and E. rugulosa CBS 171.71 and CBS 133.60 formed stable hetero ...201121858538
interactions between naturally occurring antifungal agents.pairwise interactions between four antifungal compounds were studied. the β-1,3-glucan synthase inhibitor echinocandin b (ecb) showed synergistic effect with the cell wall hydrolase chib chitinase and enga β-1,3-glucanase on saccharomyces cerevisiae, candida albicans, aspergillus rugulosus and a. fumigatus. the antifungal protein of penicillium chrysogenum (paf) did not influence the antifungal activity of chib or enga, but showed antagonistic effect with ecb on a. nidulans, a. rugulosus and a. ...201324275596
ecdghk are three tailoring iron oxygenases for amino acid building blocks of the echinocandin scaffold.the echinocandins are a small group of fungal n-acylated cyclic hexapeptides that are fungicidal for candida strains and fungistatic for aspergilli by targeting cell wall 1,3-β-glucan synthases. the side chains of all six amino acid building blocks have hydroxyl groups, including the nonproteinogenic 4r,5r-dihydroxy-orn1, 4r-oh-pro3, 3s,4s-dihydroxy-homotyr4, and 3s-oh-4s-me-pro6. the echinocandin (ecd) gene cluster contains two predicted nonheme mononuclear iron oxygenase genes (ecdg,k) and one ...201323451921
identification and characterization of the echinocandin b biosynthetic gene cluster from emericella rugulosa nrrl 11440.echinocandins are a family of fungal lipidated cyclic hexapeptide natural products. due to their effectiveness as antifungal agents, three semisynthetic derivatives have been developed and approved for treatment of human invasive candidiasis. all six of the amino acid residues are hydroxylated, including 4r,5r-dihydroxy-l-ornithine, 4r-hydroxyl-l-proline, 3s,4s-dihydroxy-l-homotyrosine, and 3s-hydroxyl-4s-methyl-l-proline. we report here the biosynthetic gene cluster of echinocandin b 1 from eme ...201222998630
rare and new etiological agents revealed among 178 clinical aspergillus strains obtained from czech patients and characterized by molecular sequencing.a collection of 178 aspergillus isolates, recovered from czech patients, mostly from 2007-2011, was subjected to multilocus dna sequence typing using the its region, β-tubulin, and calmodulin genes. an unusually wide spectrum of etiologic agents that included 36 species of aspergillus is discussed in the context of recent taxonomic and clinical reports. invasive aspergillosis (ia), onychomycosis, and otitis externa were the predominant clinical entities. five cases due to species newly proven as ...201222458252
ability of emericella rugulosa to mobilize unavailable p compounds during pearl millet [pennisetum glaucum (l.) r. br.] crop under arid condition.phosphate solubilizing microorganisms are ubiquitous in soils and could play an important role in supplying p to plants where plant unavailable p content in soil was more. a phosphatase and phytase producing fungus emericella rugulosa was isolated and tested under field condition (pearl millet as a test crop) in a loamy sand soil. in the experimental soil 68% organic phosphorous was present as phytin; less than 1% of phosphorous was present in a plant available form. the maximum effect of inocul ...200723100641
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