| a juvenile hormone-repressible transferrin-like protein from the bean bug, riptortus clavatus: cdna sequence analysis and protein identification during diapause and vitellogenesis. | we found several juvenile hormone-responsive cdnas in the bean bug, riptortus clavatus, by using mrna differential display (hirai et al., 1998). one of them, a juvenile hormone-repressible cdna, jr-3, was cloned, sequenced, characterized and identified as a transferrin (rctf). rctf cdna encoded 652 amino acids with a calculated molecular weight of 71,453 da. the deduced amino acid sequence showed significant homology with the transferin genes of several insects, manduca sexta (43% identity), bla ... | 2000 | 10790182 |
| insect-microbe mutualism without vertical transmission: a stinkbug acquires a beneficial gut symbiont from the environment every generation. | the broad-headed bug riptortus clavatus (heteroptera: alydidae) possesses a number of crypts at a posterior midgut region, which house a dense population of a bacterial symbiont belonging to the genus burkholderia. although the symbiont is highly prevalent (95 to 100%) in the host populations, the symbiont phylogeny did not reflect the host systematics at all. in order to understand the mechanisms underlying the promiscuous host-symbiont relationship despite the specific and prevalent associatio ... | 2007 | 17483286 |
| identification and cdna cloning of novel juvenile hormone responsive genes from fat body of the bean bug, riptortus clavatus by mrna differential display. | juvenile hormone (jh) induces termination of diapause and initiation of reproductive maturation in the adult female bean bug, riportus clavatus. applying pcr-based differential display, we have identified four novel jh-responsive cdnas, that is, three repressible (jr-1, 2 and 3) and one inducible (ji-1). these dna fragments were partially sequenced and compared with sequences in the data base. jr-3 was shown to have similarity to the transferrins of other insects, which have been reported as jh- ... | 1998 | 9654740 |
| 'males second' strategy in the allocation of sexes by the parasitic wasp, gryon japonicum. | patterns of the sex ratio allocation of gryon japonicum (ashmead) (hymenoptera: scelionidae), a solitary egg parasitoid of riptortus clavatus (thunberg) (heteroptera: alydidae), were investigated in the laboratory, and the result was checked against the field data on the sex composition of the parasitoid. when five host eggs were presented simultaneously to each of the females of g. japonicum in a laboratory experiment, they had a strong tendency to lay a male egg in second host egg and female e ... | 1989 | 28312529 |
| (e)-2-hexenyl hexanoate, the alarm pheromone of the bean bug riptortus clavatus (heteroptera: alydidae). | | 1992 | 27280844 |
| structural and functional studies on biliverdin-associated cyanoprotein from the bean bug, riptortus clavatus. | structural and functional analysis were performed on biliverdin-associated cyanoprotein (cp) from the hemolymph (cp-1 to cp-4) and eggs (cpegg, identical to cp-1) of the bean bug, riptortus clavatus. isoelectric focusing analysis of purified cpegg and cp-4 revealed that they are composed of a single alpha subunit and a more acidic beta subunit respectively. n-terminal amino acid sequencing revealed that there are six different amino acid residues between the two subunits up to the 29 cycles dete ... | 1994 | 7765499 |
| multifunctional communication inriptortus clavatus (heteroptera: alydidae): conspecific nymphs and egg parasitoidooencyrtus nezarae use the same adult attractant pheromone as chemical cue. | the bean bug,riptortus clavatus lays scattered eggs (as opposed to the egg masses of pentatomids) on host as well as nonhost plants. therefore, the first feeding stage (second-instar) nymphs emerging from eggs laid on nonhost plants need a signal that enables them to locate a food source at the lowest energy cost. male-released (e)-2-hexenyl (e)-2-hexenoate, (e)-2-hexenyl (z)-3-hexenoate, and myristyl isobutyrate play the double role of attractant pheromone for adults as well as aggregation pher ... | 1995 | 24234413 |
| role of the neuroendocrine complex in the control of adult diapause in the bean bug, riptortus clavatus. | in the bean bug, riptortus clavatus, allatectomy suppressed reproduction in adults reared under nondiapause-inducing long-day conditions, and transection of the nervi corporis allati induced reproduction in adults reared under diapause-inducing short-day conditions. these effects of allatectomy and denervation were observed both in the morphology of reproductive organs and in the electrophoresis pattern of hemolymph proteins in both sexes. these results indicate that, in diapause adults, the bra ... | 1997 | 9177138 |
| nucleotide sequence of vitellogenin mrna in the bean bug, riptortus clavatus: analysis of processing in the fat body and ovary. | the bean bug, riptortus clavatus, has immunologically distinct yolk proteins, vitellin (vn)-1 and-2 and their precursors, vitellogenin (vg)-1 and-2. we have cloned the full nucleotide sequence of vg-1 cdna. the deduced amino acid sequence has some similarities to other insect vgs. it contains two polyserine regions, which are characteristic of other vgs. vg-1 mrna appeared after treatment with the juvenile hormone analogue, methoprene, implying transcriptional regulation. we found four enzymatic ... | 1998 | 9753765 |
| two hexameric cyanoprotein subunits from an insect, riptortus clavatus. sequence, phylogeny and developmental and juvenile hormone regulation. | hemolymph of a hemipteran insect, riptortus clavatus, contains four distinct hexameric proteins (cyanoproteins) composed of two distinct subunits, cp alpha and cp beta, which show profiles of abundance depending on developmental stage, diapause status controlled by juvenile hormone, and sex. we have isolated cdna clones encoding the two subunits and determined the complete sequences. the nucleotide sequences predict polypeptides of 693 and 691 residues for cp alpha and cp beta, respectively, inc ... | 1998 | 9990310 |
| analysis of involvement of the 3'-untranslated regions in regulating mrna stability for vitellogenin, cyanoprotein alpha, and cyanoprotein beta from the bean bug, riptortus clavatus. | the degradation of the 3'-untranslated regions (utrs) of vitellogenin, cyanoprotein alpha, and cyanoprotein beta from the bean bug, riptortus clavatus, was analyzed in vitro. the degradation pattern was similar for all three rnas, with a high degradation rate in non-diapausing adult insects and no degradation in the fifth instar nymphs and in diapausing adults, and was not correlated with the expression levels of these three proteins. proteins binding to the 3'-utrs were detected in polysomal an ... | 2002 | 12210958 |
| changes in mechanical properties of the cuticle and lipid accumulation in relation to adult diapause in the bean bug, riptortus clavatus. | photoperiodically controlled adult diapause in the bean bug, riptortus clavatus (thunberg) (heteroptera: alydidae) was due to suppression of corpus allatum activity under short-day conditions. the mechanical extensibility of the cuticle of the pronotum was significantly higher in nondiapause adults reared under long-day conditions than in diapause adults reared under short-day conditions. furthermore, diapause adults accumulated significantly larger amount of lipids than nondiapause ones. it was ... | 1999 | 12770371 |
| effects of purified persimmon tannin and tannic acid on survival and reproduction of bean bug, riptortus clavatus. | we evaluated the effects of tannic acid and purified perrsimmon tannin on survival and reproduction of bean bugs, riptortus clavatus.feeding behavior of r. clavatus was also examined on sweet (cv. fuyu) and astringent (cv. chongdosi) persimmon fruits. soluble tannin in sweet persimmon fruits decreased from 3% in early june to 0.5% in late september, but it increased from 2 to 8% during the same period in astringent persimmon fruits. more bugs visited sweet than astringent persimmon. numbers of p ... | 2004 | 15672670 |
| heterologous expression of corn cystatin in soybean and effect on growth of the stink bug. | the bean bug (riptortus clavatus) is a serious insect pest of soybean. corn (maize) cystatin strongly inhibited the activity of its digestive cysteine proteinase. heterologous expression of corn cystatin in soybean seeds inhibited the insect's proteases, but not its growth. | 2012 | 23132569 |
| oryzacystatins exhibit growth-inhibitory and lethal effects on different species of bean insect pests, callosobruchus chinensis (coleoptera) and riptortus clavatus (hemiptera). | oryzacystatins i and ii, cysteine proteinase inhibitors in rice seeds, caused growth retardation of different species of bean insect pests, callosobruchus chinensis (coleoptera) and riptortus clavatus (hemiptera), when added to their diets at concentrations of 0.3-0.5% (w/w). at concentrations of up to 1%, almost all insects died. our results suggest the usefulness of cystatin for insect pest control and also the critical role of cysteine proteinase in the digestive events of insects. | 1996 | 27299395 |
| interspecific pheromone cross-attraction among soybean bugs (heteroptera): does piezodorus hybneri (pentatomidae) utilize the pheromone of riptortus clavatus (alydidae) as a kairomone? | the chemical and ecological function of cross-attraction of piezodorus hybneri (pentatomidae) to the riptortus clavatus (alydidae) pheromone (a mixture of three components) was studied. in a field attraction test using traps with synthetic pheromone components, p. hybneri was attracted to (e)-2-hexenyl (e)-2-hexenoate, a component of the r. clavatus pheromone. other components had neither an additive nor a synergistic effect on the attraction of p. hybneri. neither (e)-2-hexenyl (e)-2-hexenoate ... | 2006 | 16705488 |
| attraction of piezodorus hybneri to the aggregation pheromone components of riptortus clavatus. | the aggregation pheromone of riptortus clavatus (heteroptera: alydidae) consists of (e)-2-hexenyl (z)-3-hexenoate (e2hz3h), (e)-2-hexenyl (e)-2-hexenoate (e2he2h), and myristyl isobutyrate (mi). experiments were conducted to examine the attraction of piezodorus hybneri (heteroptera: pentatomidae) to the pheromone components of r. clavatus, individually and in combination, and to determine if p. hybneri produces these compounds. electroantennogram (eag) responses of p. hybneri to e2he2h were sign ... | 2006 | 16586037 |
| gut symbiotic bacteria of the genus burkholderia in the broad-headed bugs riptortus clavatus and leptocorisa chinensis (heteroptera: alydidae). | the japanese common broad-headed bugs riptortus clavatus and leptocorisa chinensis possess a number of crypts in the posterior region of the midgut, whose lumen contains a copious amount of bacterial cells. we characterized the gut symbiotic bacteria by using molecular phylogenetic analysis, light and electron microscopy, in situ hybridization, and pcr-based detection techniques. restriction fragment length polymorphism analysis of 16s rrna gene clones suggested that a single bacterium dominated ... | 2005 | 16000818 |
| genetic localization of a bruchid resistance gene and its relationship to insecticidal cyclopeptide alkaloids, the vignatic acids, in mungbean (vigna radiata l. wilczek). | bruchid resistance, controlled by a single dominant gene (br) in a wild mungbean accession (tc1966), has been incorporated into cultivated mungbean (vigna radiata). the resistance gene simultaneously confers inhibitory activity against the bean bug, riptortus clavatus thunberg (hemiptera: alydidae). the resultant isogenic line (bc20 generation) was characterized by the presence of a group of novel cyclopeptide alkaloids, called vignatic acids. a linkage map was constructed for br and the vignati ... | 1998 | 9648742 |
| cloning of mrna sequences for two antibacterial peptides in a hemipteran insect, riptortus clavatus. | escherichia coli injection rapidly induced bactericidal activity in the hemolymph of a hemipteran insect, riptortus clavatus. this activity reached its maximum at 9 hr after injection and thereafter declined slowly. two types of cdna clones involved in this response were isolated by differential screening. the predominant type encoded for an open reading frame of 678 amino acids, which consisted of fourteen tandem repeats. each repeat was rich in charged residues and had a proline-rich region wh ... | 1996 | 8688805 |