| immediate autoproteolysis and new proteinases in entamoeba invadens and entamoeba moshkovskii trophozoites. | we have examined the sodium dodecyl sulfate (sds)-induced autoproteolysis of e. invadens (pz and ip101) and e. moshkovskii (fic and laredo) trophozoite lysates. heat-treated lysates containing parahydroxy-mercuribenzoate (phmb) of all four strains had undegraded protein patterns. unheated phmb-lacking lysates of pz had two (99 and 90 kda) and ip101 lysates had three (45, 99, 90 kda) major proteins, whereas fic and laredo lysates had only one (90 kda). heat-treatment changed the remaining protein ... | 1992 | 1340331 |
| the laredo strain and other 'entamoeba histolytica-like' amoebae are entamoeba moshkovskii. | a small number of entamoeba isolates from humans, the best known of which is the 'laredo' strain, have the ability to grow at room temperature. this peculiarity, along with other characteristics, distinguishes the strains from the human pathogen e. histolytica despite their being morphologically inseparable. in contrast, these 'e. histolytica-like' strains share several features with e. moshkovskii, which is most frequently isolated from polluted water. to examine the taxonomic relationships amo ... | 1991 | 1677159 |
| antigens in electron-dense granules from entamoeba histolytica as possible markers for pathogenicity. | in vitro interaction of entamoeba histolytica with collagen induces intracellular formation and release of electron-dense granules (edg) and stimulation of collagenolytic activity. purified edg contain 1.66 u of collagenase per mg of protein. thus, edg may participate in tissue destruction during invasive amebiasis. monoclonal antibodies (mabs) l1.1 and l7.1 reacted specifically with edg in enzyme-linked immunosorbent assay (elisa) and immunofluorescence and immunoelectron microscopy. mab l7.1 i ... | 1990 | 2174899 |
| first isolation of entamoeba moshkovskii from sewage in australia. | | 1976 | 1006774 |
| [histochemical studies of various enzymes in amoebae of the genus entamoeba: entamoeba histolytica (schaudinn, 1903), entamoeba invadens (rodhain, 1934) and entamoeba moshkovskii (tshalaia, 1941)]. | | 1979 | 225891 |
| dna hybridization probe for clinical diagnosis of entamoeba histolytica. | as an alternative to microscopic identification of entamoeba histolytica parasites isolated from stool, a sensitive and species-specific dna hybridization probe was made for rapid diagnosis of e. histolytica parasites in clinical samples directly applied to nylon membranes. the dna hybridization probe was made by screening a genomic library of a virulent hm-1:imss strain of e. histolytica to detect recombinant plasmids containing highly repeated parasite dna sequences. four plasmid clones that r ... | 1989 | 2542361 |
| entamoeba histolytica-like amoebae occurring in man. | six entamoeba histolytica-like amoebae of the laredo-type are compared with classical e. histolytica strains with regard to morphology, temperature-tolerance, response to hypotonic solutions, antigenic make-up, pathogenicity, sensitivity to drugs, and biochemistry. sharp discontinuities are shown to exist between the 2 groups in some parameters, sufficient, under certain conditions, to justify creation of a new species designation for the laredo-type amoebae. nevertheless, this action is not rec ... | 1969 | 4308581 |
| [on the relations between entamoeba histolytica, laredo strains, entamoeba invadens and entamoeba moshkovskii evaluated according to their sensitivity to anti-amebic drugs]. | | 1968 | 4313543 |
| ultrastructure of entamoeba moshkovskii with particular regard to the presence of surface active lysosomes. | | 1974 | 4855311 |
| [comparative study on the ultrastructure of entamoeba moshkovskii, parasitic amoebae of the genus entamoeba, and free-living amoebae of the hartmanella-naegleria group]. | | 1974 | 4372957 |
| the influence of ph and medium osmolarity on a monoxenic culture of entamoeba moshkovskii. | | 1972 | 4661367 |
| [studies on entamoeba moshkovskii. ii. new foci in various types of water collections in brazil and uruguay]. | | 1974 | 4460182 |
| [ultrastructural aspects of entamoeba moshkovskii tshalaia, 1941]. | | 1968 | 4906624 |
| entamoeba moshkovskii: axenic cultivation. | | 1970 | 5504477 |
| the action of 5-bromo-5-nitrotetrahydro-1,3-oxazine derivatives on entamoeba moshkovskii. | | 1967 | 4875409 |
| [studies on entamoeba moshkovskii tshalaia, 1941. i. isolation in some areas of the state of minas gerais, brazil]. | | 1968 | 4884982 |
| [observations on entamoeba moshkovskii, a free-living amoeba isolated in the city of guayaquil]. | | 1965 | 5832925 |
| [studies on entamoeba moshkovskii. 3. isolation of 17 milanese strains: morphological characteristics and thermical adaptation to the environment of 4 milanese and 1 brazilian strain]. | | 1966 | 5963794 |
| isolation of entamoeba moshkovskii from the araguaia river, central brazil. | | 1971 | 5559753 |
| [the submicroscopic structure of the chromatoid body of entamoeba invadens and entamoeba moshkovskii]. | | 1967 | 5622499 |
| on the possible relationships between entamoeba moshkovskii, the laredo strains and brumpt's entamoeba dispar. | | 1969 | 5794460 |
| comparison of direct wet mount and trichrome staining techniques for detecting entamoeba species trophozoites in stools. | simulated freshly passed stools containing motile entamoeba moshkovskii trophozoites were examined by direct wet mount and permanent (trichrome) staining techniques. the percentage of detection by direct mount was 4.8%. the percentage of detection of the trophozoites by direct mount plus permanent stain was 58.5%. laboratorians should be cautioned not to rely solely on the direct wet mount for detection or identification of protozoan trophozoites. | 1980 | 6168649 |
| entamoeba moshkovskii (tshalaia, 1941): morpho-biological characterization of new strains isolated from the environment, and a review of the literature. | after an investigation carried out with samples from several sewage sludges from the town of pavia (italy) the aa. report the isolation of two new strains of entamoeba moshkovskii. the usefulness and reliability of two methods, i.e. growth in hypotonic media and the thermoresistance tests in vitro for the biological typing of this species are analyzed and discussed. both methods had already proven useful to characterize the laredo-type strains of e. histolytica too, as reported by other aa. | 1983 | 6660794 |
| entamoeba moshkovskii: a new isolate from sewage sludges in italy. | | 1982 | 7179427 |
| intraspecific variation and phylogenetic relationships in the genus entamoeba as revealed by riboprinting. | eighty-seven isolates of amebae assigned to the genus entamoeba have been studied by riboprinting (restriction enzyme polymorphism analysis of polymerase chain reaction amplified small subunit ribosomal rna genes). twenty-four distinct patterns were obtained, most of which corresponded to previously described species. in three species (entamoeba coli, entamoeba gingivalis and entamoeba moshkovskii) intraspecific variation was detected that led to the grouping of isolates into 'ribodemes' (popula ... | 1997 | 9109261 |
| lipophosphoglycan is present in distinctly different form in different entamoeba histolytica strains and absent in entamoeba moshkovskii and entamoeba invadens. | lipophosphoglycan has recently been demonstrated on the cell surface of entamoeba histolytica strain hm-1:imss. a monoclonal antibody against this molecule had failed to react with some other strains of e. histolytica, including the strain rahman. to determine if a structurally distinct lipophosphoglycan existed in rahman, [3h]galactose-labeled glycoconjugates were electrophoresed through sodium dodecyl sulfate polyacrylamide gel electrophoresis. the electrophoretic pattern in rahman was very di ... | 1995 | 7581337 |
| nucleotide sequence organisation and analysis of the nuclear ribosomal dna circle of the protozoan parasite entamoeba histolytica. | we have sequenced the extrachromsomal ribosomal dna (rdna) circle of the human protozoan parasite entamoeba histolytica hm-1:imss and present here the complete sequence organisation of the 24.5-kb molecule. each circle contains two 5.9-kb rdna transcription units organised as inverted repeats. the regions downstream (3543 bp) and upstream (9216 bp) of the rdnas contain various families of short tandem repeats. some of the upstream repeats share extensive sequence homology with the downstream rep ... | 1994 | 7870125 |
| heterogeneity of the ribosomal dna episome in strains and species of entamoeba. | ribosomal dna sequences in several species of the genus entamoeba are highly repeated and display restriction fragment-length polymorphism (rflp), which has been used to identify species and differentiate strains. however, the continuous variability of the non-transcribed repeat sequences in the ribosomal episome hinders an accurate typification. looking for more reliable markers, we used dna probes containing conserved sequences in the ribosomal episome--coding regions for the 16s and 5.8s rrna ... | 1994 | 7934884 |
| endoplasmic reticulum and golgi-like elements in entamoeba. | the cytoplasm of entamoeba is characterized by the presence of a large number of vesicles of different size and shape. previous electron microscopic studies have not clearly revealed the presence of the endoplasmic reticulum and the golgi complex. in the present study two approaches were used aimed at the identification of these two structures in trophozoites of entamoeba moshkovskii and entamoeba histolytica: (a) cytochemical techniques associated with transmission electron microscopy, such as ... | 1997 | 9377074 |
| ecto-nucleotide triphosphatase activity in pathogenic and non-pathogenic entamoeba: protection from the cytotoxic effects of extracellular atp. | the nucleotide triphosphatase (ntpase) activity of entamoeba species and of entamoeba histolytica strains, was compared. in all cases, with the exception of entamoeba moshkovskii, the enzyme was activated by ca2+ and not by mg2+ and preferentially hydrolysed utp with decreasing activity for atp and gtp. the ntpase activity was associated with both the intracellular and the plasma membrane sulphonylbenzoyl-adenosine (fsba) of trophozoites in which the atp-site was facing externally, as shown by f ... | 1997 | 9670549 |
| entamoeba moshkovskii infections in children, bangladesh. | entamoeba moshkovskii cysts are morphologically indistinguishable from those of the disease-causing species e. histolytica and the nonpathogenic e. dispar. although sporadic cases of human infection with e. moshkovskii have been reported, the organism is considered primarily a free-living amoeba. no simple molecular detection tool is available for diagnosing e. moshkovskii infections. we used polymerase chain reaction (pcr) to detect e. moshkovskii directly in stool. we tested 109 stool specimen ... | 2003 | 12737742 |
| inter- and intra-strain variation in the 5.8s ribosomal rna and internal transcribed spacer sequences of entamoeba histolytica and comparison with entamoeba dispar, entamoeba moshkovskii and entamoeba invadens. | the ribosomal rna genes in entamoeba histolytica are located on circular dna molecules in about 200 copies per genome equivalent. nucleotide sequence analysis of the 5.8s rrna gene and the flanking internal transcribed spacers was carried out to determine the degree of sequence divergence in the multiple rrna gene copies of a given strain; amongst three different e. histolytica strains (hm-1:imss, rahman and hk-9); and amongst four species of entamoeba (entamoeba histolytica, entamoeba dispar, e ... | 2000 | 10856506 |
| molecular cloning and characterization of peroxiredoxin from entamoeba moshkovskii. | | 2000 | 11070226 |
| [observations on erythrocyte phagocytosis by entamoeba moshkovskii as reported by tshalaia, 1941]. | | 1956 | 13396011 |
| ehp53, an entamoeba histolytica protein, ancestor of the mammalian tumour suppressor p53. | this paper reports the identification of ehp53, a p53-like entamoeba histolytica protein, which binds to the human p53 dna consensus sequence (oli-p53). monoclonal antibodies against p53 (ab-1 and ab-2) recognized a single 53 kda spot in two-dimensional gels and inhibited the formation of complexes produced by e. histolytica nuclear extracts and oli-p53. additionally, e. histolytica gene promoter sequences with high homology to oli-p53 formed complexes with nuclear proteins that were abolished b ... | 2003 | 12686631 |
| studies on the morphology and biology of entamoeba moshkovskii tshalaia, 1941. | | 1953 | 13111812 |
| [entamoeba moshkovskii as a convenient didactic object]. | | 1956 | 13312254 |
| [2 strains of entamoeba moshkovskii (czalaja, 1941) in the coastal zone]. | | 1960 | 13714616 |
| unexpected diversity and differential success of dna transposons in four species of entamoeba protozoans. | we report the first comprehensive analysis of transposable element content in the compact genomes (approximately 20 mb) of four species of entamoeba unicellular protozoans for which draft sequences are now available. entamoeba histolytica and entamoeba dispar, two human parasites, have many retrotransposons, but few dna transposons. in contrast, the reptile parasite entamoeba invadens and the free-living entamoeba moshkovskii contain few long interspersed elements but harbor diverse and recently ... | 2005 | 15901838 |
| entamoeba histolytica: observations on metabolism based on the genome sequence. | the sequencing of the genome of entamoeba histolytica has allowed a reconstruction of its metabolic pathways, many of which are unusual for a eukaryote. based on the genome sequence, it appears that amino acids may play a larger role than previously thought in energy metabolism, with roles in both atp synthesis and nad regeneration. arginine decarboxylase may be involved in survival of e. histolytica during its passage through the stomach. the usual pyrimidine synthesis pathway is absent, but a ... | 2005 | 15955308 |
| [the sensitivity of entamoeba moshkovskii tshalia to salts of new n-halogenamides of benzenesulphonic acid and analogous compounds]. | | 1964 | 14346907 |
| detection and identification of entamoeba species in stool samples by a reverse line hybridization assay. | classically, detection of entamoeba histolytica is performed by microscopic examination for characteristic cysts and/or trophozoites in fecal preparations. differentiation of e. histolytica cysts and those of nonpathogenic amoebic species is made on the basis of the appearance and the size of the cysts. however, by classical means objective tools for confirmation and quality control do not exist. therefore, a reverse line blot hybridization assay was developed to detect a variety of entamoeba sp ... | 2003 | 14605136 |
| molecular characterization of peroxiredoxin from entamoeba moshkovskii and a comparison with entamoeba histolytica. | peroxiredoxin of the pathogenic parasite, entamoeba histolytica, is thought to be involved in protection from oxidative attack by host phagocytic cells and endogenously generated hydrogen peroxide. in this study, we cloned peroxiredoxin genes from the nonpathogenic ameba, entamoeba moshkovskii, and characterized the peroxiredoxin protein. the open reading frame of three cloned cdnas was demonstrated to encode a polypeptide of 218 or 217 amino acids. identity of the amino acid sequence of peroxir ... | 2004 | 15555731 |
| entamoeba moshkovskii and entamoeba dispar-associated infections in pondicherry, india. | the prevalence of laredo strain--entamoeba moshkovskii--and non-pathogenic e. dispar in patients attending the jawaharlal institute of postgraduate medical education and research hospital, pondicherry, india, is reported here. e. moshkovskii is reported for the first time in india. the species are morphologically indistinguishable from pathogenic e. histolytica. of 746 stool samples screened, 68 showing cyst or trophozoite stage of e. histolytica, e. dispar, or e. moshkovskii were subjected to s ... | 2005 | 16262027 |
| two cases of rarely recognized infection with entamoeba moshkovskii. | we report two cases (a 36-year-old woman and 2-year-old girl) infected with entamoeba moshkovskii in turkey. entamoeba moshkovskii was identified and distinguished from the morphologically identical parasites e. histolytica and e. dispar by a nested polymerase chain reaction and restriction fragment length polymorphism analysis of the small subunit ribosomal dna gene. | 2007 | 17426178 |
| differential detection of entamoeba histolytica, entamoeba dispar, and entamoeba moshkovskii by a single-round pcr assay. | a single-round pcr assay was developed for detection and differential diagnosis of the three entamoeba species found in humans, entamoeba moshkovskii, entamoeba histolytica, and entamoeba dispar, that are morphologically identical as both cysts and trophozoites. a conserved forward primer was derived from the middle of the small-subunit rrna gene, and reverse primers were designed from signature sequences specific to each of these three entamoeba species. pcr generates a 166-bp product with e. h ... | 2006 | 16954247 |
| new insights into the phylogeny of entamoeba species provided by analysis of four new small-subunit rrna genes. | sequences of small-subunit rrna genes have been obtained for four new isolates of entamoeba. phylogenetic analyses give new insights into the evolution of these organisms. a novel entamoeba from pigs in vietnam that produces uninucleate cysts proved to be unrelated to other uninucleated cyst-producing species. revival of the name entamoeba suis for this organism is proposed. instead of being related to entamoeba polecki, it shares a recent common ancestor with the non-encysting entamoeba gingiva ... | 2006 | 16957127 |
| entamoeba moshkovskii infections in sydney, australia. | entamoeba moshkovskii is considered to be a free-living ameba, which is morphologically similar, but biochemically and genetically different, to entamoeba histolytica and entamoeba dispar. however, recent studies have suggested that e. moshkovskii may be a "potential" pathogen, with infections giving rise to diarrhea and other intestinal disorders. microscopy and polymerase chain reaction (pcr) targeting the 18s ribosomal (r) dna was performed on fecal samples collected from patients presenting ... | 2008 | 17957394 |
| pcr detection of entamoeba histolytica, entamoeba dispar, and entamoeba moshkovskii in stool samples from sydney, australia. | this study investigated the presence of entamoeba histolytica, entamoeba dispar, and entamoeba moshkovskii in stool samples from a patient population in sydney, australia. stool samples were tested by microscopy and pcr. five patients were found with e. histolytica infections, while e. dispar and e. moshkovskii were observed in 63 (70.8%) and 55 (61.8%) patients, respectively, by pcr. this is the first study in australia using molecular techniques to determine the presence of e. histolytica, e. ... | 2007 | 17229864 |
| comparison of stool antigen detection kits to pcr for diagnosis of amebiasis. | the present study was conducted to compare two stool antigen detection kits with pcr for the diagnosis of entamoeba histolytica infections by using fecal specimens submitted to the department of microbiology at st. vincent's hospital, sydney, and the institute of medical and veterinary science, adelaide, australia. a total of 279 stool samples containing the e complex (e. histolytica, entamoeba dispar, and entamoeba moshkovskii) were included in this study. the stool specimens were tested by usi ... | 2008 | 18367563 |
| high prevalence of entamoeba moshkovskii in a tanzanian hiv population. | entamoeba moshkovskii and entamoeba dispar are microscopically indistinguishable from the pathogenic species entamoeba histolytica. there are limited data on the prevalence of these commensal infections from africa. we utilized pcr and antigen detection to evaluate the carriage rate of e. moshkovskii, e. dispar, and e. histolytica infection in stool from a cohort of hiv-suspected or confirmed inpatients from tanzania. e. histolytica was detected by elisa in 4% (5/118) while e. moshkovskii and e. ... | 2008 | 18471796 |
| laboratory diagnostic techniques for entamoeba species. | the genus entamoeba contains many species, six of which (entamoeba histolytica, entamoeba dispar, entamoeba moshkovskii, entamoeba polecki, entamoeba coli, and entamoeba hartmanni) reside in the human intestinal lumen. entamoeba histolytica is the causative agent of amebiasis and is considered a leading parasitic cause of death worldwide in humans. although recent studies highlight the recovery of e. dispar and e. moshkovskii from patients with gastrointestinal symptoms, there is still no convin ... | 2007 | 17630338 |
| diagnosis of intestinal amoebiasis by using nested polymerase chain reaction-restriction fragment length polymorphism assay. | microscopy is unreliable to distinguish the pathogenic entamoeba histolytica from the nonpathogenic entamoeba dispar or entamoeba moshkovskii in stool specimens. | 2007 | 17701126 |
| patterns of evolution in the unique trna gene arrays of the genus entamoeba. | genome sequencing of the protistan parasite entamoeba histolytica hm-1:imss revealed that almost all the trna genes are organized into tandem arrays that make up over 10% of the genome. the 25 distinct array units contain up to 5 trna genes each and some also encode the 5s rna. between adjacent genes in array units are complex short tandem repeats (strs) resembling microsatellites. to investigate the origins and evolution of this unique gene organization, we have undertaken a genome survey to de ... | 2008 | 17974548 |
| reassessment of the epidemiology of amebiasis: state of the art. | the epidemiology of amebiasis has dramatically changed since the separation of entamoeba histolytica and entamoeba dispar species, and the worldwide prevalence of these species has not been estimated until recently. the most cited data regarding prevalence, morbidity, or mortality due to amebiasis is the 1986 walsh report, in which 100,000 deaths are reported to occur worldwide each year due to medical complications of invasive amebiasis. however, the prevalence values of entamoeba histolytica i ... | 2009 | 19540361 |
| mutation detection analysis of a region of 16s-like ribosomal rna gene of entamoeba histolytica, entamoeba dispar and entamoeba moshkovskii. | the level of intra-species genetic variation in entamoeba histolytica, entamoeba dispar and entamoeba moshkovskii populations in a localized geographic area, like puducherry, india, remains unknown. | 2008 | 18822136 |
| first molecular identification of entamoeba moshkovskii in human stool samples in tunisia. | we report the first intestinal infections in tunisia with entamoeba moshkovskii in two healthy adults. entamoeba moshkovskii cysts were distinguished from those of the morphologically identical parasites entamoeba histolytica and entamoeba dispar by specific nested polymerase chain reaction and sequencing. | 2008 | 18981508 |
| differential identification of entamoeba spp. based on the analysis of 18s rrna. | entamoeba histolytica is known to cause intestinal and extra-intestinal disease while the other entamoeba species are not considered to be pathogenic. however, all entamoeba spp. should be reported when identified in clinical samples. entamoeba polecki, entamoeba coli, and entamoeba hartmanii can be differentiated morphologically from e. histolytica, but some of their diagnostic morphologic features overlap. e. histolytica, entamoeba dispar, and entamoeba moshkovskii are morphologically identica ... | 2010 | 20169364 |
| molecular epidemiology of amebiasis. | entamoeba histolytica, the causative agent of human amebiasis, remains a significant cause of morbidity and mortality in developing countries and is responsible for up to 100,000 deaths worldwide each year. entamoeba dispar, morphologically indistinguishable from e. histolytica, is more common in humans in many parts of the world. similarly entamoeba moshkovskii, which was long considered to be a free-living ameba, is also morphologically identical to e. histolytica and e. dispar, and is highly ... | 2008 | 18571478 |
| discrimination of entamoeba moshkovskii in patients with gastrointestinal disorders by single-round pcr. | entamoeba moshkovskii and entamoeba dispar are impossible to differentiate microscopically from the pathogenic species entamoeba histolytica. there are limited data on the prevalence of these commensal parasites in iran. we utilized a single-round pcr assay to determine the prevalence of e. moshkovskii, e. dispar, and e. histolytica in stool samples from iranian patients infected with gastrointestinal disorders. after culturing of microscopy-positive isolates and extraction of dna, pcr was carri ... | 2010 | 20332579 |
| evaluation of a new single-tube multiprobe real-time pcr for diagnosis of entamoeba histolytica and entamoeba dispar. | a single-tube multiprobe real-time pcr assay for simultaneous detection of entamoeba histolytica and entamoeba dispar was developed. one primer pair with 2 species-specific probes was designed based on new ssu rna regions of the ribosomal dna-containing episome. the sensitivity is 1 parasite per milliliter of feces and thus superior to the conventional nested pcr and comparable to other published real-time pcr protocols. the applicability for clinical diagnosis was validated with 218 stool speci ... | 2010 | 20496961 |
| [amebiasis: implications of the recognition of entamoeba dispar and the identification of entamoeba moshkovskii in humans]. | the history of entamoeba histolytica is very confuse and shows several wrong concepts about the parasite and its relationship with the host. the poor correlation between the prevalence of asymptomatic and symptomatic amebiasis originated the proposal of three explicative hypothesis, among them was the concept of brumpt that e. histolytica comprised two morphologically identical species, e. dysenteriae and e. dispar. the application of modern molecular techniques irrefutably proved that e. histol ... | 2010 | 20928980 |
| identification and delineation of members of the entamoeba complex by pyrosequencing. | a method using a single-round pcr coupled to pyrosequencing was developed for the detection and differentiation of members of the entamoeba complex. the technique was evaluated using dna isolated directly from faecal specimens and compared with a duplex real-time pcr targeting entamoeba histolytica and entamoeba dispar, and a conventional single-round pcr for the detection of entamoeba moshkovskii. tetranucleate cysts from 102 faecal specimens from swedish, danish and dutch patients test-positiv ... | 2010 | 20691255 |
| Public health and clinical importance of amoebiasis in Malaysia: a review. | Entamoeba histolytica, the causative agent of human amoebiasis remains a significant cause of morbidity and mortality in developing countries and is responsible for up to 100,000 deaths worldwide each year. Entamoeba dispar, morphologically indistinguishable from E. histolytica is more common in humans in many parts of the world. Similarly Entamoeba moshkovskii, which was long considered to be a free-living amoeba is also morphologically identical to E. histolytica and E. dispar, and is highly p ... | 2011 | 22041740 |
| polymerase chain reaction confirmation of diagnosis of intestinal amebiasis in puducherry. | stool microscopy, the commonly used test for diagnosis of intestinal amebiasis, is unreliable as it does not differentiate entamoeba histolytica, the causative agent for amebiasis, from non-pathogenic entamoeba dispar and entamoeba moshkovskii. | 2010 | 20814775 |
| development of multiplex real-time polymerase chain reaction for detection of entamoeba histolytica, entamoeba dispar, and entamoeba moshkovskii in clinical specimens. | multiplex real-time polymerase chain reaction (pcr) was developed for differential detection of entamoeba histolytica, entamoeba dispar, and entamoeba moshkovskii. specific primers were designed for all three species, and then differentiation of e. histolytica and e. dispar was achieved simultaneously using a hybridization probe and melting curve analysis, whereas e. moshkovskii was detected with a separate probe under the same condition. this assay detected as little as 0.2 pg of e. histolytica ... | 2010 | 20889890 |
| intestinal parasites in immigrants in the city of naples (southern italy). | the present study was aimed at carrying out a cross-sectional copromicroscopic survey of helminths and intestinal protozoa in immigrants in naples (southern italy). between october 2008 and november 2009, a total of 514 immigrants were tested comparing the flotac dual technique and the ethyl acetate concentration technique. combined results of the two techniques served as a diagnostic 'gold' standard and revealed an overall prevalence of parasitic infections of 61.9% (318/514). the ethyl acetate ... | 2010 | 21195044 |
| molecular identification of entamoeba species in savanna woodland chimpanzees (pan troglodytes schweinfurthii). | to address the molecular diversity and occurrence of pathogenic species of the genus entamoeba spp. in wild non-human primates (nhp) we conducted molecular-phylogenetic analyses on entamoeba from wild chimpanzees living in the issa valley, tanzania. we compared the sensitivity of molecular [using a genus-specific polymerase chain reaction (pcr)] and coproscopic detection (merthiolate-iodine-formaldehyde concentration) of entamoeba spp. we identified entamoeba spp. in 72 chimpanzee fecal samples ... | 2016 | 26935395 |
| molecular diagnosis of entamoeba spp. versus microscopy in the great cairo. | amoebiasis is a human disease produced by entamoeba histolytica which causes widespread mortality and morbidity worldwide through diarrheal disease and abscess establishment in parenchymal tissues such as liver, lung, and brain. the true prevalence of infection is unknown for most areas of the world due to the difficulty to characterise entamoeba histolytica versus other non-pathogenic amoebas with identical morphology, as entamoeba dispar, and entamoeba moshkovskii. to overcome microscopy misid ... | 2017 | 28030342 |
| gene migration for re-emerging amebiasis in iran's northwest-iraq borders: a microevolutionary scale for reflecting epidemiological drift of entamoeba histolytica metapopulations. | in the microevolutionary scales of entamoeba isolates, the gene migration shows how entamoeba spp. has epidemiologically drifted among border countries. five hundred fecal samples were taken from patients suffering gastrointestinal disorders, abdominal pain, and diarrhea at saggez, northwest iran located within the border iraq country. following parasitological techniques, dna samples were extracted and amplified by polymerase chain reaction (pcr) of 18s rrna region to identify entamoeba infecti ... | 2017 | 27761718 |
| frequency and molecular characterisation of entamoeba histolytica, entamoeba dispar, entamoeba moshkovskii, and entamoeba hartmanni in the context of water scarcity in northeastern brazil. | this study aimed to estimate the frequency, associated factors, and molecular characterisation of entamoeba histolytica, entamoeba dispar, entamoeba moshkovskii, andentamoeba hartmanni infections. we performed a survey (n = 213 subjects) to obtain parasitological, sanitation, and sociodemographic data. faecal samples were processed through flotation and centrifugation methods.e. histolytica, e. dispar, e. moshkovskii, and e. hartmanni were identified by nested-polymerase chain reaction (pcr). th ... | 2016 | 26841049 |
| molecular epidemiology of amoebiasis: a cross-sectional study among north east indian population. | epidemiological studies carried out using culture or microscopy in most of the amoebiasis endemic developing countries, yielded confusing results since none of these could differentiate the pathogenic entamoeba histolytica from the non-pathogenic entamoeba dispar and entamoeba moshkovskii. the northeastern part of india is a hot spot of infection since the climatic conditions are most conducive for the infection and so far no systemic study has been carried out in this region. | 2015 | 26633890 |
| intestinal amebae. | among the entamoeba species that infect humans, entamoeba histolytica causes diseases, entamoeba dispar is a harmless commensal, entamoeba moshkovskii seems to be a pathogen, and the pathogenicity of entamoeba bangladeshi remains to be investigated. species-specific detection needed for treatment decisions and for understanding the epidemiology and pathogenicity of these amebae. antigen-based detection methods are needed for e dispar, e moshkovskii, and e bangladeshi; and molecular diagnostic te ... | 2015 | 26004649 |
| application of multiplex pcr for detection and differentiation of entamoeba histolytica, entamoeba dispar and entamoeba moshkovskii. | entamoeba moshkovskii and e. dispar are impossible to differentiate microscopically from the pathogenic species e. histolytica. multiplex polymerase chain reaction (multiplex pcr) is a widespread molecular biology technique for amplification of multiple targets in a single pcr experiment. | 2017 | 25759727 |
| bioconjugated fluorescent silica nanoparticles for the rapid detection of entamoeba histolytica. | rapid detection of entamoeba histolytica based on fluorescent silica nanoparticle (fsnp) indirect immunofluorescence microscopy was evaluated. silica nanoparticles were synthesized using stöber's method, with their surface activated to covalently bind to, and immobilize, protein a. for biolabeling, fsnp was added to conjugated e. histolytica trophozoites with monoclonal anti-e. histolytica igg1 for microscopic observation of fluorescence. fluorescent silica nanoparticle sensitivity was determine ... | 2015 | 25697865 |
| entamoeba bangladeshi: an insight. | molecular tools have the potential to differentiate microscopically similar gut micro-eukaryotes that may have significantly different relationships with the human host. using broad range entamoeba primers to amplify a section of the eukaryotic 18s small subunit ribosomal rna gene a novel member of the entamoeba family (entamoeba bangladeshi) has recently been identified. the goal of this review is to place this species in the context of what is already known about this genus and to discuss the ... | 2014 | 25250229 |
| laboratory methods of identification of entamoeba histolytica and its differentiation from look-alike entamoeba spp. | entamoeba histolytica, the causative agent of intestinal and extraintestinal amebiasis, is a common parasitic cause of significant morbidity and mortality in the developing countries. hence, early detection and differentiation of pathogenic e. histolytica from nonpathogenic/commensal entamoeba spp (entamoeba dispar/entamoeba moshkovskii/entamoeba bangladeshi) plays a crucial role in clinical management of patients with amebiasis. most diagnostic tests currently available do not reliably differen ... | 2014 | 25250228 |
| development of a thermostabilised triplex lamp assay with dry-reagent four target lateral flow dipstick for detection of entamoeba histolytica and non-pathogenic entamoeba spp. | this study highlighted the development of a four target nitrocellulose-based nucleic acid lateral flow immunoassay biosensor in a dry-reagent strip format for interpretation of double-labelled double-stranded amplicons from thermostabilised triplex loop-mediated isothermal amplification assay. the dna biosensor contained two test lines which captured biotin and texas red labelled amplicons; a lamp internal amplification control line that captured digoxigenin labelled amplicon; and a chromatograp ... | 2017 | 28372729 |
| entamoeba moshkovskii is associated with diarrhea in infants and causes diarrhea and colitis in mice. | entamoeba moshkovskii is prevalent in developing countries and morphologically indistinguishable from pathogenic entamoeba histolytica and nonpathogenic entamoeba dispar. it is not known if e. moshkovskii is pathogenic. | 2012 | 22723640 |
| different clinical outcomes of entamoeba histolytica in malaysia: does genetic diversity exist? | the present study was conducted to investigate the clinical outcomes of entamoeba histolytica infection in symptomatic and asymptomatic orang asli (aborigine) communities in malaysia. examination was performed on 500 stool samples obtained from orang asli communities in 3 different states using formalin-ether concentration, trichrome staining, and single-round pcr techniques. out of 500 stool samples, single infection of e. histolytica, entamoeba dispar, and entamoeba moshkovskii was identified ... | 2013 | 23710093 |
| differential detection of entamoeba histolytica, entamoeba dispar and entamoeba moshkovskii in fecal samples by nested pcr in the united arab emirates (uae). | amoebiasis is one of the most important infectious diseases afflicting mainly tropical and subtropical countries. this study was carried out in the sharjah emirate, uae in order to accurately detect and differentiate entamoeba histolytica, entamoeba dispar and e. moshkovskii in fecal samples collected from the sharjah municipality public health clinic by elisa and nested polymerase chain reaction (pcr). one hundred and twenty specimens were examined and the pcr was positive for e. histolytica, e ... | 2013 | 23666654 |
| prevalence of entamoeba nuttalli infection in wild rhesus macaques in nepal and characterization of the parasite isolates. | we have recently resurrected the name entamoeba nuttalli castellani, 1908 for a potentially virulent ameba isolate, p19-061405, obtained from a rhesus macaque in kathmandu, nepal. the ameba was morphologically indistinguishable from entamoeba histolytica/entamoeba dispar/entamoeba moshkovskii, but located phylogenetically between e. histolytica and e. dispar. to evaluate the prevalence of e. nuttalli infection in wild rhesus macaques, 112 fecal samples were collected in four locations of the kat ... | 2013 | 23370534 |
| prevalence and risk factors associated with entamoeba histolytica/dispar/moshkovskii infection among three orang asli ethnic groups in malaysia. | entamoeba histolytica/entamoeba dispar/entamoeba moshkovskii infection is still prevalent in rural malaysia especially among orang asli communities. currently, information on prevalence of this infection among different ethnic groups of orang asli is unavailable in malaysia. to contribute to a better comprehension of the epidemiology of this infection, a cross-sectional study aimed at providing the first documented data on the prevalence and risk factors associated with e. histolytica/e. dispar/ ... | 2012 | 23133561 |
| molecular epidemiology of amoebiasis in malaysia: highlighting the different risk factors of entamoeba histolytica and entamoeba dispar infections among orang asli communities. | currently, species-specific information on entamoeba infections is unavailable in malaysia and is restricted worldwide due to the re-description of pathogenic entamoeba histolytica and non-pathogenic entamoeba dispar and entamoeba moshkovskii. therefore, this cross-sectional study was conducted to provide the first known documented data on the true prevalence of these three species in western malaysia using a molecular method. another aim of this study was to determine the association of potenti ... | 2012 | 23123168 |
| differentiating entamoeba histolytica, entamoeba dispar and entamoeba moshkovskii using nested polymerase chain reaction (pcr) in rural communities in malaysia. | in this study, a total of 426 human faecal samples were examined for the presence of entamoeba histolytica, entamoeba dispar, entamoeba moshkovskii infection via a combination of microscopic examination and nested polymerase chain reaction (pcr) targeting 16s ribosomal rna of entamoeba species. | 2012 | 22947430 |
| first molecular identification of entamoeba moshkovskii in malaysia. | entamoeba moshkovskii and entamoeba dispar are microscopically indistinguishable from the pathogenic species entamoeba histolytica. although sporadic cases of human infection with e. moshkovskii have been reported, the amoeba is still considered primarily as a free-living amoeba. a cross-sectional study was carried out among orang asli communities in 3 different states of peninsular malaysia. fecal samples were examined by formalin-ether sedimentation and trichrome staining techniques and then s ... | 2012 | 22939193 |
| molecular epidemiology of entamoeba: first description of entamoeba moshkovskii in a rural area from central colombia. | entamoeba histolytica, e. dispar and e. moshkovskii are the most frequent species described in human infection where e. histolytica is the only true pathogen. the epidemiology of this infection is complex due to the absence of a routine exam that allows a correct discrimination of the entamoeba species complex. therefore, molecular methods appear as the unique epidemiological tool to accomplish the species discrimination. herein, we conducted a cross-sectional study to determine the frequency of ... | 2015 | 26465744 |
| occurrence of giardia, cryptosporidium, and entamoeba in wild rhesus macaques (macaca mulatta) living in urban and semi-rural north-west india. | giardia duodenalis, cryptosporidium spp., and entamoeba spp. are intestinal protozoa capable of infecting a range of host species, and are important causes of human morbidity and mortality. understanding their epidemiology is important, both for public health and for the health of the animals they infect. this study investigated the occurrence of these protozoans in rhesus macaques (macaca mulatta) in india, with the aim of providing preliminary information on the potential for transmission of t ... | 2017 | 28229045 |
| [an update on amebiasis]. | the description of entamoeba dispar, and the recovery of entamoeba moshkovskii from humans had a major impact in the epidemiology and clinical management of amebiasis. infections range from asymptomatic colonization to hemorrhagic colitis and extra-intestinal diseases. only a minority of amebiasis patients progress to the development of disease. recent studies suggest that susceptibility to infection, and its outcome is influenced by the host, parasite genotype, and environment. the identificati ... | 2013 | 24089276 |
| real-time pcr assay in differentiating entamoeba histolytica, entamoeba dispar, and entamoeba moshkovskii infections in orang asli settlements in malaysia. | amebiasis caused by entamoeba histolytica is the third leading cause of death worldwide. this pathogenic amoeba is morphologically indistinguishable from e. dispar and e. moshkovskii, the non-pathogenic species. polymerase chain reaction is the current method of choice approved by world health organization. real-time pcr is another attractive molecular method for diagnosis of infectious diseases as post-pcr analyses are eliminated and turnaround times are shorter. the present work aimed to compa ... | 2013 | 23985047 |
| species-specific immunity induced by infection with entamoeba histolytica and entamoeba moshkovskii in mice. | entamoeba histolytica, the parasitic amoeba responsible for amoebiasis, causes approximately 100,000 deaths every year. there is currently no vaccine against this parasite. we have previously shown that intracecal inoculation of e. histolytica trophozoites leads to chronic and non-healing cecitis in mice. entamoeba moshkovskii, a closely related amoeba, also causes diarrhea and other intestinal disorders in this model. here, we investigated the effect of infection followed by drug-cure of these ... | 2013 | 24312397 |
| molecular epidemiology and genetic diversity of entamoeba species in a chelonian collection. | veterinary medicine has focused recently on reptiles, due to the existence of captive collections in zoos and an increase in the acquisition of reptiles as pets. the protozoan parasite, entamoeba can cause amoebiasis in various animal species and humans. although amoebiasis disease is remarkably rare in most species of chelonians and crocodiles, these species may serve as entamoeba species carriers that transmit parasites to susceptible reptile species, such as snakes and lizards, which can beco ... | 2014 | 24194557 |
| entamoeba moshkovskii perspectives of a new agent to be considered in the diagnosis of amebiasis. | during the last decade entamoeba moshkovskii has become relevant given its capacity to infect humans, especially when considering that it is morphologically indistinguishable from e. histolytica. for a long time, e. moshkovskii was considered as a free living amoeba, but in the last decade it has been demonstrated that e. moshkovskii can infect humans and can be found more frequently in regions where amebiasis shows high prevalence values, becoming a challenge to differentiate it from the e. his ... | 2012 | 22664420 |
| identification of entamoeba moshkovskii in treated waste water used for agriculture. | we conducted an observational study to determine the prevalence of entamoeba spp., in samples collected in a waste water treatment plant that provides water for agricultural irrigation. samples were collected weekly over a period of 10 weeks at representative contamination stages from within the treatment plant. protozoan identification was performed via light microscopy and culture. pcr amplification of small subunit rrna gene sequences of e. histolytica/dispar/moshkovskii was performed in cult ... | 2016 | 26732073 |