| feulgen type staining of mammalian tissues with dahlia-so2. | the use of the basic dye, dahlia, which belongs to triphenylmethane group but without a primary amino group in its molecule has been described as useful in the staining of aldehyde groups of acid hydrolysed dna in tissue sections following the conventional feulgen procedure. dahlia-so2 prepared with sodium hydrosulphite is highly suitable when used at ph 4-0 to 5-0. the absorption characteristics of the stained nuclei indicate on the peak of maximum absorption at 560 nm, whereas, that of the aqu ... | 1977 | 72716 |
| hoffman's violet and dahlia as specific stains for animal chromosomes. | the paper deals with staining of the chromosomes of animal testicular materials with two basic dyes, hoffman's violet and dahlia of the triphenylmethane group, following iodine-dye procedure. the important finding, as presented herein, is that iodinated alcohol after staining can be substituted with various acids, both organic as well as inorganic, all of which act as trapping agent preventing leaching of the dye that binds with the chromosomal dna. it is clear from this study that rna is not in ... | 1979 | 86937 |
| sensitivity to dahlia flowers. | | 1978 | 149640 |
| osmotic effects on radial growth rate and specific growth rate of three soil fungi. | the radial growth rate on osmotically adjusted agar medium and the relative specific growth rate in osmotically adjusted liquid medium were determined for rhizoctonia solani, pythium ultimum, and verticillium dahliae. on basal medium, an isolate of p. ultimum and r. solani had similar radial growth rates of 0.52 and 0.47 mm/h, respectively, whereas v. dahliae grew at a rate of 0.08 mm/h. radial growth rate was reduced 50% at osmotic potentials of -16, -27, and -32 bars for p. ultimum, r. solani, ... | 1978 | 570448 |
| assay of nitrogenase activity in intact plant systems. | nitrogenase activity was assayed in intact system of cichorium intybus, a non-leguminous commercially cultivated crop, dahlia pinnata and helianthus annus, and taraxacum officinale, a common weed plant. the assay was made in fabricated cylinders which could accomodate pot with plants. in such kind of assay along with rhizosphere microflora, the nitrogen fixed by phyllosphere nitrogen fixing microflora could also be accounted, which otherwise was difficult to be accounted for. | 1975 | 1211718 |
| contact dermatitis from dahlia pinnata. | | 1990 | 2149333 |
| phytoalexin synthesis in soybean cells: elicitor induction of reductase involved in biosynthesis of 6'-deoxychalcone. | chromatofocusing on mono p proved to be an efficient purification procedure for the nadph-dependent reductase from soybean (glycine max l.) cell cultures which acts together with chalcone synthase in the biosynthesis of 2',4',4-trihydroxychalcone (6'-deoxychalcone). by isoelectric focusing the pi of reductase was determined to be 6.3. addition of pure soybean reductase to cell-free extracts from stimulated cell cultures of parsley and bean (phaseolus vulgaris) and from young flowers of dahlia va ... | 1989 | 2500065 |
| airborne contact dermatitis from compositae plants in northern india. | 60 patients (45 male and 15 female) with suspected airborne contact dermatitis from compositae (asteraceae) plants and 20 age-matched controls (15 male and 5 female) were investigated. patch tests were performed with ethanolic plant extracts of 4 compositae plants, parthenium hysterophorus, chrysanthemum morifolium, dahlia pinnata and tagetes indica, all prevalent in northern india. 55 (92%) patients showed positive patch tests, with 35 (64%) demonstrating positive tests to extracts of only 1 of ... | 1989 | 2805654 |
| the isolation of a toxin from the dahlia sea anemone, tealia felina l. | gel chromatography with dyematrex blue b gel and ion exchange chromatography with a methylcellulose cation exchange column have been used to isolate a toxin present in partially purified saline extracts of the dahlia sea anemone tealia felina. toxin activity was assessed by its histaminolytic activity. a ten-fold increase in activity per mg protein was achieved in these two steps. final purification on a sephacryl column yielded an apparently pure protein with a molecular weight of 7800. the ami ... | 1986 | 2871642 |
| purification and characterization of (+)dihydroflavonol (3-hydroxyflavanone) 4-reductase from flowers of dahlia variabilis. | individual flowers from inflorescences of dahlia variabilis (cv scarlet star) in young developmental stages contained relatively high activity of (+)-dihydroflavonol (dhf) 4-reductase. the dhf reductase was purified from such flowers to apparent homogeneity by a five-step procedure. this included affinity adsorption on blue sepharose and elution of the enzyme with nadp+. by gel filtration and by sodium dodecyl sulfate-polyacrylamide gel electrophoresis it was shown that dhf reductase contains on ... | 1988 | 3293532 |
| effect of so2 on growth and development of dahlia rosea cav. | | 1988 | 3382791 |
| compositae dermatitis in childhood. | compositae dermatitis occurred in a 9-year-old boy with a strong personal and family history of atopy. positive patch test reactions were 2+ for dandelion (taraxacum officinale), false ragweed (ambrosia acanthicarpa), giant ragweed (ambrosia trifida), short ragweed (ambrosia artemisifolia), sagebrush (artemisia tridentata), wild feverfew (parthenium hysterophorus), yarrow (achillea millifolium), and tansy (tanacetum vulgare) and 1+ for dahlia species and english ivy (hedera helix). patch tests w ... | 1987 | 3827282 |
| the medicinal and nutritional properties of dahlia spp. | the common garden variety dahlia was once an important root crop and medicinal plant among the pre-columbian indians of central mexico, yucatan and guatemala. its roots were valued both for the nutritious inulin stored inside them and for the antibiotic compounds concentrated in the skin of the tubers. the dahlia flower was a solar symbol worn by moctezuma and his nobles. in the modern world, dahlias cultivated as a crop might prove to be a worthy food supplement in subtropical areas. | 1985 | 3910964 |
| fine structure of zinnial leaf tissues infected with dahlia mosaic virus. | | 1969 | 4186523 |
| [adaptation of dahlia mosaic virus to zinnia, and production of antisera for serodiagnostic purposes]. | | 1965 | 5876925 |
| partial purification, morphology, and serology of dahlia mosaic virus. | | 1966 | 5938895 |
| use of aqueous solutions of two basic dyes for the demonstration of dna. | this paper presents informations as to the ability of aqueous solutions of two basic dyes, such as dahlia and victoria blue, belonging to aminotriarylmethane group for the staining of dna-aldehyde molecules as well as dna-phosphate groups. it has been found that sections of rat tissues stained with aqueous solutions of these dyes after acid hydrolysis followed by drying between folds of filter paper and treatment in n-butanol for a minute and then by a very brief treatment in a mixture consistin ... | 1982 | 6175884 |
| isolation and characterisation of plant defensins from seeds of asteraceae, fabaceae, hippocastanaceae and saxifragaceae. | from seeds of aesculus hippocastanum, clitoria ternatea, dahlia merckii and heuchera sanguinea five antifungal proteins were isolated and shown to be homologous to plant defensins previously characterised from radish seeds and gamma-thionins from poaceae seeds. based on the spectrum of their antimicrobial activity and the morphological distortions they induce on fungi the peptides can be divided into two classes. the peptides did not inhibit any of three different alpha-amylases. | 1995 | 7628617 |
| pcr protocol- and inulin catabolism-based differentiation of inulinolytic soil bacteria. | bacteria collected from rotting dahlia tubers, instead of degrading inulin to d-fructose, preferentially formed the known dfa iii (beta-2.1': alpha-2',3 difructofuranose anhydride), inulobiose, higher inulo-oligosaccharides, and exoheteropolysaccharides. owing to the morphological and gram staining variability, the bacterial isolates designated ylw and crm were examined to differentiate them from a reference strain arthrobacter ureafaciens. the comparative analyses were whole dna random amplific ... | 1994 | 8010763 |
| rats discriminate between starch and other substances having a similar texture. | studies examined the contribution of textural factors to the ability of rats to sense starch. if rats sense the abrasive effects of starch suspensions, conditioned aversions to starch should generalize to substances having a similar texture. two substances having a texture similar to that of starch were examined, polymeric dialdehyde (a product derived from starch) and inulin (a polymer of fructose). rats were trained to avoid dilute suspensions of rice starch, potato starch, polymeric dialdehyd ... | 1993 | 8446700 |
| production of high concentrations of ethanol from inulin by simultaneous saccharification and fermentation using aspergillus niger and saccharomyces cerevisiae. | pure nonhydrolyzed inulin was directly converted to ethanol in a simultaneous saccharification and fermentation process. an inulinase-hyperproducing mutant, aspergillus niger 817, was grown in a submerged culture at 30 degrees c for 5 days. the inulin-digestive liquid culture (150 ml) was supplemented with 45 g of inulin, 0.45 g of (nh4)2so4, and 0.15 g of kh2po4. the medium (ph 5.0) was inoculated with an ethanol-tolerant strain, saccharomyces cerevisiae 1200, and fermentation was conducted at ... | 1993 | 8481000 |
| fungal membrane responses induced by plant defensins and thionins. | treatment of hyphae of neurospora crassa with antifungal plant defensins, i.e. rs-afp2 and dm-amp1 isolated from radish and dahlia seed, respectively, induced a rapid k+ efflux, ca2+ uptake, and alkalinization of the incubation medium. the rs-afp2-induced alkalinization of the incubation medium could be inhibited with g-protein inhibitors. alpha-hordothionin, an antifungal thionin from barley seed, caused a sustained increased ca2+ uptake at subinhibitory concentrations but only a transient incr ... | 1996 | 8663029 |
| the coronary vasoconstrictor action of extract iv from the dahlia sea anemone tealia felina l. | extract iv, a partially purified preparation of the toxin from the sea anemone tealia felina, produced marked bradycardia and arrhythmias in the rat in vivo. in the langendorff rat heart preparation perfused at constant pressure extract iv (0.0224 au/ml) reduced the force of contraction by 81.3 +/- 7.2%, n = 6, and the coronary flow by 82.0 +/- 4.7%, n = 5. when the preparation was perfused at constant flow rate, extract iv (0.072 au/ml) increased the coronary circulation resistance from 3.76 +/ ... | 1996 | 8711761 |
| analysis of fructans from higher plants by matrix-assisted laser desorption/ionization mass spectrometry. | in this communication both matrix-assisted laser desorption/ionization mass spectrometry (maldi-ms) and high-performance anion-exchange chromatography (hpaec) have been applied to analyze fructans from higher plants. size distribution of a commercially available fructan preparation from dahlia variabilis l. was determined by maldi-ms. molecular masses ranged from 2,000 up to 6,000 da with a peak value of distribution at 2,635 da. essentially the same pattern was obtained using hpaec. low-molecul ... | 1997 | 9073356 |
| measurement of glomerular filtration rate using inulin prepared from vernonia herbacea, a brazilian native species. | vernonia herbacea (vell.) rusby (asteraceae) is a perennial herb native to the cerrado vegetation of tropical areas in brazil, which accumulates inulin in the underground reserve organs. the aim of this paper was to determine whether the inulin extracted from v. herbacea could replace commercial inulin for the measurement of glomerular filtration rate (gfr). underground organs of vegetative plants were collected from a preserved area of the brazilian cerrado. the inulin fraction utilized was obt ... | 1996 | 9181115 |
| permeabilization of fungal membranes by plant defensins inhibits fungal growth. | we used an assay based on the uptake of sytox green, an organic compound that fluoresces upon interaction with nucleic acids and penetrates cells with compromised plasma membranes, to investigate membrane permeabilization in fungi. membrane permeabilization induced by plant defensins in neurospora crassa was biphasic, depending on the plant defensin dose. at high defensin levels (10 to 40 microm), strong permeabilization was detected that could be strongly suppressed by cations in the medium. th ... | 1999 | 10584003 |
| plant fructans stabilize phosphatidylcholine liposomes during freeze-drying. | fructans have been implicated as protective agents in the drought and freezing tolerance of many plant species. a direct proof of their ability to stabilize biological structures under stress conditions, however, is still lacking. here we show that inulins (linear fructose polymers) isolated from chicory roots and dahlia tubers stabilize egg phosphatidylcholine large unilamellar vesicles during freeze-drying, while another polysaccharide, hydroxyethyl starch, was completely ineffective. liposome ... | 2000 | 10632723 |
| specific binding sites for an antifungal plant defensin from dahlia (dahlia merckii) on fungal cells are required for antifungal activity. | dm-amp1, an antifungal plant defensin from seeds of dahlia (dahlia merckii), was radioactively labeled with t-butoxycarbonyl-[35s]-l-methionine n-hydroxy-succinimi-dylester. this procedure yielded a 35s-labeled peptide with unaltered antifungal activity. [35s]dm-amp1 was used to assess binding on living cells of the filamentous fungus neurospora crassa and the unicellular fungus saccharomyces cerevisiae. binding of [35s]dm-amp1 to fungal cells was saturable and could be competed for by preincuba ... | 2000 | 10656585 |
| a gene encoding a sphingolipid biosynthesis enzyme determines the sensitivity of saccharomyces cerevisiae to an antifungal plant defensin from dahlia (dahlia merckii). | we have previously identified a saccharomyces cerevisiae mutant that is markedly more resistant than wild-type to dahlia merckii antimicrobial peptide 1 (dmamp1), an antifungal plant defensin isolated from seeds of dahlia (dahlia merckii). a complementation approach was followed that consisted of the introduction of a genomic library of dmamp1-sensitive wild-type yeast into the dmamp1-resistant yeast mutant and screening for restored sensitivity to dmamp1. the gene determining sensitivity of s. ... | 2000 | 10931938 |
| screening for microorganisms that produce only endo-inulinase. | sixteen fungal strains reported in the literature as endo-inulinase producers and three bacterial strains, isolated from the dahlia rhizosphere, were analysed for endo-inulinase production. from four isolated strains (one fungus and three bacteria) the results were evaluated in terms of substrate consumption, cell growth and production of endo-inulinases. all three bacterial strains were sole endo-inulinase producers and, among these, strain paenibacillus sp. cdb 003 was the most suitable for en ... | 2001 | 11414332 |
| transgenic expression in arabidopsis of a polyprotein construct leading to production of two different antimicrobial proteins. | we developed a method for expression in arabidopsis of a transgene encoding a cleavable chimeric polyprotein. the polyprotein precursor consists of a leader peptide and two different antimicrobial proteins (amps), dmamp1 originating from dahlia merckii seeds and rsafp2 originating from raphanus sativus seeds, which are linked by an intervening sequence ("linker peptide") originating from a natural polyprotein occurring in seed of impatiens balsamina. the chimeric polyprotein was found to be clea ... | 2002 | 11950983 |
| antimycotic activities of selected plant flora, growing wild in lebanon, against phytopathogenic fungi. | petroleum ether (pe) and methanolic extracts of nine wild plant species were tested in vitro for their antimycotic activity against eight phytopathogenic fungi. the efficacy of pe extracts against all pathogens tested was higher than that of methanolic extracts. wild marjoram (origanum syriacum) pe extract showed the highest and widest range of activity. it resulted in complete inhibition of mycelial growth of six of eight fungi tested and also gave nearly complete inhibition of spore germinatio ... | 2002 | 12009988 |
| cdna cloning, heterologous expressions, and functional characterization of malonyl-coenzyme a:anthocyanidin 3-o-glucoside-6"-o-malonyltransferase from dahlia flowers. | in the flowers of important ornamental compositae plants, anthocyanins generally carry malonyl group(s) at their 3-glucosyl moiety. in this study, for the first time to our knowledge, we have identified a cdna coding for this 3-glucoside-specific malonyltransferase for anthocyanins, i.e. malonyl-coenzyme a:anthocyanidin 3-o-glucoside-6"-o-malonyltransferase, from dahlia (dahlia variabilis) flowers. we isolated a full-length cdna (dv3mat) on the basis of amino acid sequences specifically conserve ... | 2002 | 12481098 |
| effects of alternative dietary substrates on competition between human colonic bacteria in an anaerobic fermentor system. | duplicate anaerobic fermentor systems were used to examine changes in a community of human fecal bacteria supplied with different carbohydrate energy sources. a panel of group-specific fluorescent in situ hybridization probes targeting 16s rrna sequences revealed that the fermentors supported growth of a greater proportion of bacteroides and a lower proportion of gram-positive anaerobes related to faecalibacterium prausnitzii, ruminococcus flavefaciens-ruminococcus bromii, eubacterium rectale-cl ... | 2003 | 12571040 |
| dmamp1, an antifungal plant defensin from dahlia (dahlia merckii), interacts with sphingolipids from saccharomyces cerevisiae. | dmamp1, an antifungal plant defensin from dahlia merckii, was shown previously to require the presence of sphingolipids for fungicidal action against saccharomyces cerevisiae. sphingolipids may stabilize glycosylphosphatidylinositol (gpi)-anchored proteins, which interact with dmamp1, or they may directly serve as dmamp1 binding sites. in the present study, we demonstrate that s. cerevisiae disruptants in gpi-anchored proteins showed small or no increased resistance towards dmamp1 indicating no ... | 2003 | 13129623 |
| the flavonoid glycosides of dahlia variabilis. ii. glycosides of yellow varieties pius ix and coton. | | 1956 | 13292910 |
| the flavonoid glycosides of dahlia variabilis. iii. glycosides from white varieties. | | 1958 | 13498771 |
| uridine diphosphate fructose and uridine diphosphate acetylgalactosamine from dahlia tubers. | | 1963 | 13949032 |
| inclusion bodies in dahlia infected with dahlia mosaic virus. | | 1963 | 14098498 |
| location, structure, and cytochemical staining reactions of the inclusion bodies found in dahlia variabilis infected with dahlia mosaic virus. | | 1964 | 14192297 |
| [research on plant viruses. virus card ix: chlorotic vein banding of the dahlia (dahlia pinnata cav.)]. | | 1964 | 14221465 |
| [attempts at extraction of dahlia mosaic virus (marmor dahliae holmes) and electron microscopic examination of the products obtained]. | | 1951 | 14857444 |
| enrichment of higher molecular weight fractions in inulin. | inulin (general formulas gfn and fm, with g = anhydroglucose and f = anhydrofructose) naturally occurs as a homologous series of oligo- and polysaccharides with different chain lengths. for reasons of growing interest in the food and pet food industries, the short chain inulins have to be separated from their long chain analogues because their properties (digestibility, prebiotic activity and health promoting potential, caloric value, sweetening power, water binding capacity, etc.) differ substa ... | 2004 | 15186097 |
| alexander the great, the dahlia, and the tortoise. | some of the problems of establishing the cause of the death of alexander the great are like the attempts to find causes other than hysteria for anna o.'s symptoms. the more general problem of using plausibility as a criterion of the truth of such reconstructions are illustrated by the arguments embedded in tom stoppard's arcadia. | 2004 | 15370321 |
| innovative research from a rural practice: a blue dahlia? | | 2004 | 15588269 |
| molecular properties and prebiotic effect of inulin obtained from artichoke (cynara scolymus l.). | a high molecular weight inulin has been prepared from artichoke (cynara scolymus l.) agroindustrial wastes using environmentally benign aqueous extraction procedures. physico-chemical analysis of the properties of artichoke inulin was carried out. its average degree of polymerization was 46, which is higher than for jerusalem artichoke, chicory, and dahlia inulins. gc-ms confirmed that the main constituent monosaccharide in artichoke inulin was fructose and its degradation by inulinase indicated ... | 2005 | 15960982 |
| cloning and functional analysis of a high dp fructan:fructan 1-fructosyl transferase from echinops ritro (asteraceae): comparison of the native and recombinant enzymes. | inulin-type fructans are the simplest and most studied fructans and have become increasingly popular as prebiotic health-improving compounds. a natural variation in the degree of polymerization (dp) of inulins is observed within the family of the asteraceae. globe thistle (echinops ritro), artichoke (cynara scolymus), and viguiera discolor biosynthesize fructans with a considerably higher dp than cichorium intybus (chicory), helianthus tuberosus (jerusalem artichoke), and dahlia variabilis. the ... | 2006 | 16449376 |
| [identification of dahlia mosaic virus with molecular-biological methods]. | | 2006 | 16523706 |
| contribution of phla and some metabolites of fluorescent pseudomonads to antifungal activity. | fluorescent pseudomonas species are an important group of pgpr that suppress fungal root and seedling disease by production of antifungal metabolites such as 2,4-diacetylphloroglucinol (2,4-dapg), pyoluteorin, pyrolinitrin, siderophores and hcn. the compound 2,4-dapg is a major determinant in biocontrol of plant pathogens. a 7.2 kbp chromosomal dna region, carrying dapg biosynthetic genes (phla, phlc, phlb, phld, phie and phlf). detecting the ph1 genes make them an ideal marker gene for 2,4-dapg ... | 2005 | 16637170 |
| [analysis of genetic variation in unisexual and bisexual lizard species of the genus leiolepis from southeast asia]. | using multilocus dna fingerprinting with microsatellite probes (cac)5, (gaca)4, (ggca)4 and (gata)4, intraspecific variation of the southeast asian lizards belonging to the genus leiolepis (bisexual species leiolepis reevesii and triploid parthenogenetic species leiolepis guentherpetersi) was first examined. the l. guentherpetersi lizards were characterized by monophyletic dna fingerprint profiles for the loci detected by the (gaca)4, (ggca)4, and (cac)5 probes, in terms of intrapopulation simil ... | 2006 | 16808237 |
| yellow flowers generated by expression of the aurone biosynthetic pathway. | flower color is most often conferred by colored flavonoid pigments. aurone flavonoids confer a bright yellow color on flowers such as snapdragon (antirrhinum majus) and dahlia (dahlia variabilis). a. majus aureusidin synthase (amas1) was identified as the key enzyme that catalyzes aurone biosynthesis from chalcones, but transgenic flowers overexpressing amas1 gene failed to produce aurones. here, we report that chalcone 4'-o-glucosyltransferase (4'cgt) is essential for aurone biosynthesis and ye ... | 2006 | 16832053 |
| optimization of medium and process parameters for the production of inulinase from a newly isolated kluyveromyces marxianus ys-1. | a newly isolated strain of kluyveromyces marxianus ys-1 was used for the production of extra cellular inulinase in a medium containing inulin, meat extract, cacl2 and sodium dodecyl sulphate (sds). fermentation medium ph 6.5, cultivation temperature 30 degrees c and 5% (v/v) inoculum of 12 h-old culture were optimal for enzyme production (30.8 iu/ml) with a fermentation time of 72 h at shake flask level. raw inulin (2%, w/v) extracted from dahlia tubers by processing at 15 kg/cm2 for 10 min was ... | 2007 | 17071081 |
| ectopic expression of dahlia merckii defensin dmamp1 improves papaya resistance to phytophthora palmivora by reducing pathogen vigor. | phytophthora spp., some of the more important casual agents of plant diseases, are responsible for heavy economic losses worldwide. plant defensins have been introduced as transgenes into a range of species to increase host resistance to pathogens to which they were originally susceptible. however, the effectiveness and mechanism of interaction of the defensins with phytophthora spp. have not been clearly characterized in planta. in this study, we expressed the dahlia merckii defensin, dmamp1, i ... | 2007 | 17216480 |
| antifungal pisum sativum defensin 1 interacts with neurospora crassa cyclin f related to the cell cycle. | plant defensins, components of the plant innate immune system, are cationic cysteine-rich antifungal peptides. evidence from the literature [thevissen, k., et al. (2003) peptides 24, 1705-1712] has demonstrated that patches of fungi membrane containing mannosyldiinositolphosphorylceramide and glucosylceramides are selective binding sites for the plant defensins isolated from dahlia merckii and raphanus sativus, respectively. whether plant defensins interact directly or indirectly with fungus int ... | 2007 | 17240982 |
| genome structure and organization of a member of a novel and distinct species of the genus caulimovirus associated with dahlia mosaic. | the genome structure and organization of a new and distinct caulimovirus that is widespread in dahlia (dahlia variabilis) was determined. the double-stranded dna genome was ca. 7.0 kb in size and shared many of the features of the members of the genus caulimovirus, such as the presence of genes potentially coding for the movement protein, the inclusion body protein, and the reverse transcriptase (rt), and an intergenic region consisting of a potential 35s promoter. however, the virus differed fr ... | 2008 | 18253696 |
| a new and distinct species in the genus caulimovirus exists as an endogenous plant pararetroviral sequence in its host, dahlia variabilis. | viruses in certain genera in family caulimoviridae were shown to integrate their genomic sequences into their host genomes and exist as endogenous pararetroviral sequences (eprv). however, members of the genus caulimovirus remained to be the exception and are known to exist only as episomal elements in the infected cell. we present evidence that the dna genome of a new and distinct caulimovirus species, associated with dahlia mosaic, is integrated into its host genome, dahlia (dahlia variabilis) ... | 2008 | 18462770 |
| enzymatic hydrolysis of inulin to fructose by glutaraldehyde fixed yeast cells. | inulin, a polyfruction, is found as the reserve carbohydrate in the roots and tubers of various plants (i.e. jerusalem artichoke, chicory, and dahlia tubers). the beta-fructofuranosidase (inulase) from the yeast kluyveromyces fragilis is of interest because of its industrial potential in fructose syrup and alcohol production from inulin containing plants. we have found that the inulase of k. fragilis can be immobilized in the yeast cells by glutaraldehyde treatment. these cells are resistant to ... | 1984 | 18553476 |
| [amplification and cloning of dahlia mosaic virus and carnation etched ring virus promoters]. | amplification and cloning of dahlia mosaic virus promoter were carried out for the first time. sequence analysis showed homology between this promoter and the promoters of other caulimoviruses. in addition, amplification and cloning of the carnation etched ring virus promoter was performed. | 2007 | 18592695 |
| expression of dm-amp1 in rice confers resistance to magnaporthe oryzae and rhizoctonia solani. | magnaporthe oryzae and rhizoctonia solani, are among the most important pathogens of rice, severely limiting its productivity. dm-amp1, an antifungal plant defensin from dahlia merckii, was expressed in rice (oryza sativa l. sp. indica cv. pusa basmati 1) using agrobacterium tumefaciens-mediated transformation. expression levels of dm-amp1 ranged from 0.43% to 0.57% of total soluble protein in transgenic plants. it was observed that constitutive expression of dm-amp1 suppresses the growth of m. ... | 2009 | 18618285 |
| physical maps of the genomes of dahlia mosaic virus and mirabilis mosaic virus-two members of the caulimovirus group. | the nucleic acids of dahlia mosaic virus (damv) and mirabilis mosaic virus (mmv) have been isolated and compared with the native dna of cauliflower mosaic virus (camv). the native dnas of these viruses separated into circular and linear molecules during gel electrophoresis to produce patterns nearly identical to those of camv. the dnas of damv and mmv were cloned in bacteria and used for mapping the cleavage sites for 14 different restriction endonucleases. these sites were confirmed with native ... | 1983 | 18644277 |
| use of heteroduplex mobility assay for identification and differentiation of phytoplasmas in the aster yellows group and the clover proliferation group. | abstract this paper describes the identification and differentiation of phytoplasmas by a highly sensitive diagnostic technique, dna heteroduplex mobility assay (hma). closely related phytoplasma isolates of clover proliferation (cp), potato witches'-broom (pwb), and alfalfa witches'-broom (awb) were collected from the field from 1990 to 1999. the entire 16s rrna gene and 16/23s spacer region were amplified by polymerase chain reaction (pcr) from the field samples and standard cp, pwb, and awb p ... | 2001 | 18943942 |
| nucleocapsid gene-mediated transgenic resistance provides protection against tomato spotted wilt virus epidemics in the field. | abstract transformation of plants with the nucleocapsid (n) gene of tomato spotted wilt tospovirus (tswv) provides resistance to disease development; however, information is lacking on the response of plants to natural inoculum in the field. three tobacco cultivars were transformed with the n gene of a dahlia isolate of tswv (tswv-d), and plants were evaluated over several generations in the greenhouse. the resistant phenotype was more frequently observed in 'burley 21' than in 'ky-14' or 'k-326 ... | 2000 | 18944602 |
| kinetic spectrophotometric determination of trace manganese (ii) with dahlia violet in nonionic microemulsion medium. | a new catalytic kinetic spectrophotometric method has been developed for the determination of trace amount of manganese (ii) in nonionic microemulsion medium. the method is based on the catalytic effect of manganese (ii) on the oxidation of dahlia violet by potassium periodate with nitrilotriacetic acid as an activitor in the presence of nonionic microemulsion. under the optimum conditions, the calibration graph is linear in the range of 0.0004-0.0056 mug ml(-1) of manganese (ii) at 580 nm. the ... | 2003 | 18968906 |
| nucleotide sequence and genome organization of a member of a new and distinct caulimovirus species from dahlia. | a distinct caulimovirus, associated with dahlia mosaic, was cloned and sequenced. the caulimovirus, tentatively designated as dahlia common mosaic virus (dcmv), had a double-stranded dna genome of ca. 8 kb. the genome organization of dcmv was found to be typical of members of the genus caulimovirus and consisted of six major open reading frames (orfs), orfs i-vi, and one minor orf, orf vii. sequence comparisons with the dna genomes of two known caulimoviruses isolated from dahlia, dahlia mosaic ... | 2008 | 18974923 |
| effect of different growing media on the growth and development of dahlia (dahlia pinnata) under the agro-climatic condition of dera ismail khan. | an experiment was conducted to investigate the effect of different growing media on the growth and development of dahlia (dahlia pinnata). seven different growing media including sand, silt, leaf mold, sand + silt, sand + leaf mold, silt + leaf mold and sand + silt + leaf mold, were used to check the growth and development of dahlia in pots. the over all best performance was recorded in media sand + silt + leaf mold for almost all the parameters; plant height (42.08 cm), stem thickness (1.93 cm) ... | 2007 | 19090295 |
| use of conserved genomic regions and degenerate primers in a pcr-based assay for the detection of members of the genus caulimovirus. | the genus caulimovirus consists of several distinct virus species with a double-stranded dna genome that infect diverse plant species. a comparative analysis of the sequences of known caulimovirus species revealed two regions that are conserved in all caulimovirus species with the exception of strawberry vein banding virus. degenerate primers based on these two regions were designed and tested in a polymerase chain reaction-based assay for broad spectrum detection of members of this genus. cauli ... | 2009 | 19100290 |
| allelic variants from dahlia variabilis encode flavonoid 3'-hydroxylases with functional differences in chalcone 3-hydroxylase activity. | in the petals of dahlia variabilis, hydroxylation of chalcones at position 3 can be detected, except the well-known flavonoid 3'-hydroxylation. although the reaction is well characterized at the enzymatic level, it remained unclear whether it is catalyzed by a flavonoid 3'-hydroxylase (f3'h, ec1.14.13.21, cyp75b) with broad substrate specificity. two novel allelic variants of f3'h were cloned from d. variabilis, which differ only in three amino acids within their 508 residues. the corresponding ... | 2010 | 19931222 |
| biodiversity and new records of microfungi in the ruhrarea (north rhine westfalia), germany. | during our investigations of the microflora in nrw (duisburg, düsseldorf and essen incl. the greenhouse of the botanical garden) in 2007 and 2008, we were able to collect and identify about 55 species on trees, bushes and ornamental plants as parasites and saprophytes. some of these species are new for germany or have been only rarely found until now. most of the species belong the ascomycotina, basidiomycotina and deuteromycotina for example arthrocladiella mougeotii (lév.) vassilkov. on lycium ... | 2009 | 20222564 |
| evaluation of the performance of single-walled carbon nanohorns in capillary electrophoresis. | this paper describes for the first time the use of single-walled carbon nanohorns (swnhs) as pseudostationary and stationary phases for ekc and cec, respectively, taking advantage of their characteristic features, such as conical-end termination, formation of spherical assemblies dahlia-flower like superstructure and easy functionalization. the use of swnhs as pseudostationary phase for ekc required the study of their dispersion in different surfactants as well as their compatibility with the el ... | 2010 | 20419702 |
| biotechnological potential of inulin for bioprocesses. | inulin consists of linear chains of β-2,1-linked d-fructofuranose molecules terminated by a glucose residue through a sucrose-type linkage at the reducing end. in this review article, inulin and its applications in bioprocesses are overviewed. the tubers of many plants, such as jerusalem artichoke, chicory, dahlia, and yacon contain a large amount of inulin. inulin can be actively hydrolyzed by microbial inulinases to produce fructose, glucose and inulooligosaccharides (ios). the fructose and gl ... | 2010 | 21247760 |
| the dahlia mosaic virus gene vi product n-terminal region is involved in self-association. | the genome of the floriculture pathogen dahlia mosaic caulimovirus (dmv) encodes six open reading frames. generally, caulimovirus gene vi products (p6s) are thought to be multifunctional proteins required for viral infection and it is likely that self-association is required for some of these functions. in this study, yeast two-hybrid and maltose binding protein (mbp) pull-down assays indicated that full-length dmv p6 specifically self-associates. further analyses indicated that only the dmv p6 ... | 2011 | 21571015 |
| [effects of drought stress on physiological and biochemical parameters of dahlia pinnata]. | taking dahlia pinnata 'fenxishi' as test material, this paper studied its leaf physiological and biochemical responses to different degrees of drought stress and re-watering. with the increasing extent and duration of drought stress, the leaf relative water content, water potential, and chlorophyll content of d. pinnata 'fenxishi' decreased significantly, leaf relative electric conductivity and malondialdehyde (mda) content had a significant increase, plasma membrane was damaged, and massive ion ... | 2011 | 21657020 |
| fructo-oligosaccharide production from inulin through partial citric or phosphoric acid hydrolyses. | abstract purified inulin from dahlia tubers was partially hydrolyzed to form fructo-oligosaccharides by using citric or phosphoric acids (ph, 2.0-2.5) as mild acid catalysts. the ideal kinetic conditions to ensure a high yield of fructo-oligosaccharides relative to free fructose were a temperature range of 85┬░c-95┬░c, a hydrolysis time of 15-25 minutes, and a catalyst ph of 2.5. at the higher temperature and the longest hydrolysis time, an inversion of the product ratio occurred. under these co ... | 2011 | 21663491 |
| a bhlh transcription factor, dvivs, is involved in regulation of anthocyanin synthesis in dahlia (dahlia variabilis). | dahlias (dahlia variabilis) exhibit a wide range of flower colours because of accumulation of anthocyanin and other flavonoids in their ray florets. two lateral mutants were used that spontaneously occurred in 'michael j' (mjw) which has yellow ray florets with orange variegation. mjor, a bud mutant producing completely orange ray florets, accumulates anthocyanins, flavones, and butein, and mjy, another mutant producing completely yellow ray florets, accumulates flavones and butein. reverse tran ... | 2011 | 21765172 |
| simultaneous post-transcriptional gene silencing of two different chalcone synthase genes resulting in pure white flowers in the octoploid dahlia. | garden dahlias (dahlia variabilis) are autoallooctoploids with redundant genes producing wide color variations in flowers. there are no pure white dahlia cultivars, despite its long breeding history. however, the white areas of bicolor flower petals appear to be pure white. the objective of this experiment was to elucidate the mechanism by which the pure white color is expressed in the petals of some bicolor cultivars. a pigment analysis showed that no flavonoid derivatives were detected in the ... | 2011 | 21688014 |
| genomic characterization of pararetroviral sequences in wild dahlia spp. in natural habitats. | the genome structure and organization of endogenous caulimovirus sequences from dahlia (dahlia spp), dahlia mosaic virus (dmv)-d10 from three wild species, d. coccinea (d10-dc), d. sherffii (d10-ds) and d. tenuicaulis (d10-dt), were determined and compared to those from cultivated species of dahlia, d. variabilis (dveprs). the complete ca. 7-kb dsdna genomes of d10-dc, d10-ds, and d10-dt had a structure and organization typical of a caulimovirus and shared 89.3 to 96.6% amino acid sequence ident ... | 2011 | 21837417 |
| Complete genome sequence of a dahlia common mosaic virus isolate from New Zealand. | Dahlia mosaic disease of the ornamental flowering plant Dahlia is caused by two caulimoviruses, dahlia mosaic virus (DMV) and dahlia common mosaic virus (DCMV). We used a rolling-circle amplification method to amplify, clone and determine for the first time the full genome sequence of a DCMV isolate from New Zealand (DCMV-NZ). Within the 7949-bp circular double-stranded retro-transcribing DCMV-NZ DNA, we identified six putative open reading frames, typical of all genomes in the family Caulimovir ... | 2011 | 21960043 |
| understanding the effect of flower extracts on the photoconducting properties of nanostructured tio2. | here we report an easy method to improve the optoelectronic properties of commercially available tio2 nanopowder using extracts of various flowers viz. calendula orange (co), calendula yellow (cy), dahlia violet (dv), dahlia yellow (dy), rabbit flower (rf), sweet poppy (sp), sweet williams (sw) and their mixed extracts (me). various analysis techniques such as uv-vis, ftir, fesem, xrd, and raman spectroscopy were used to characterize for elemental, structural and morphological properties of the ... | 2012 | 23421149 |
| [construction and pathogenicity tests of a mutated specific fragment scf73 in verticillium dahliae]. | to identify preliminarily the specific fragment scf73's function in verticillium dahlia virulence. | 2012 | 23383504 |