| the complete nucleotide sequence of yam mosaic virus (ivory coast isolate) genomic rna. | the complete nucleotidic sequence of the yam mosaic virus (ymv) rna was determined following the cloning of partial segments of the genome by reverse transcription and polymerase chain reactions (rt-pcr) using degenerate and/or specific oligonucleotide primers. ymv genomic rna is 9,608 nucleotides in length and contains one open reading frame (orf) encoding a polyprotein of 3,103 amino acids (aa) with a calculated mr of 350,915. the 5' leader sequence of ymv rna preceding the orf is 134 nucleoti ... | 1996 | 8774686 |
| nucleotide sequence of coat protein gene of yam mild mosaic virus, isolated in papua new guinea. | we determined the 3'-termimus 1353 nucleotides (nts) in length excluding the poly (a) tail of yam mild mosaic potyvirus (ymmv) rna. the sequence starts within a long open reading frame (orf) 1209 nts and is followed by untranslated region (3'-utr) of 144 nts. the coat protein (cp) contains 266 amino acids (aa) with molecular ratio (mr) of approximately 30 kda. the cp of ymmv differs substantially from yam mosaic virus (ymv), japanese yam mosaic virus (jymv) (57 and 61% of amino acid sequence ide ... | 1999 | 10481747 |
| evidence for diversifying selection in potato virus y and in the coat protein of other potyviruses. | the modes of evolution of the proteins of potato virus y were investigated with a maximum-likelihood method based on estimation of the ratio between non-synonymous and synonymous substitution rates. evidence for diversifying selection was obtained for the 6k2 protein (one amino acid position) and coat protein (24 amino acid positions). amino acid sites in the coat proteins of other potyviruses (bean yellow mosaic virus, yam mosaic virus) were also found to be under diversifying selection. most o ... | 2002 | 12237440 |
| potyviruses, novel and known, in cultivated and wild species of the family apiaceae in australia. | three potyviruses were identified by gene sequencing and found to be widespread in species of apiaceae in australia. only celery mosaic virus was found in celery crops and in one of 180 specimens of feral carrot ( daucus carota). another related but distinct novel potyvirus, carrot virus y, was the only virus found in carrot crops and all except one feral carrot. a more distantly related novel potyvirus, apium virus y, was found in plants of sea celery ( apium prostratum), cultivated parsley ( p ... | 2002 | 12376749 |
| recognition of novel viral sequences that associate with the dynein light chain lc8 identified through a pepscan technique. | recent data from multiple laboratories indicate that upon infection, many different families of viruses hijack the dynein motor machinery and become transported in a retrograde manner towards the cell nucleus. in certain cases, one of the dynein light chains, lc8, is involved in this interaction. using a library of overlapping dodecapeptides synthesized on a cellulose membrane (pepscan technique) we have analyzed the interaction of the dynein light chain lc8 with 17 polypeptides of viral origin. ... | 2003 | 12782328 |
| daphne mosaic virus (dapmv), a new potyvirus from daphne mezereum in the czech republic. | daphne shrubs with light green rings and mosaic on leaves contained flexuous filamentous virions (696 x 13 nm) and cylindrical inclusions typical of the subdivision iii of edwardson's classification for inclusions induced by members of the family potyviridae. decoration tests using antisera to 67 potyviruses revealed distant serological relations among chilli veinal mottle virus, colombian datura virus, papaya ringspot virus, tobacco vein mottling virus and yam mosaic virus. the 3' terminal regi ... | 2006 | 16292598 |
| konjak mosaic virus: the complete nucleotide sequence of the genomic rna and its comparison with other potyviruses. | konjak mosaic virus (komv) belongs to the genus potyvirus, family potyviridae. the complete nucleotide sequence of komv f isolate (komv f) was determined. the genome is 9,544 nucleotides long excluding the 3' terminal poly a tail and encodes a typical potyviral 350-kda polyprotein of 3,087 amino acids. phylogenetic analysis using known potyvirus polyproteins shows that komv constitutes a branch with yam mosaic virus, close to another branch including japanese yam mosaic virus, turnip mosaic viru ... | 2006 | 16538420 |
| production of yam mosaic virus (ymv)-free dioscorea opposita plants by cryotherapy of shoot-tips. | in the present study, yam mosaic virus (ymv) could be efficiently eliminated by cryotherapy in dioscorea opposita. shoot apices were precultured for 16 h with 0.3 m sucrose, encapsulated in sodium alginate and dehydrated for 4 h prior to direct immersion in liquid nitrogen. up to 90 percent of the plants regenerated from cryopreserved shoot tips were ymv-free, whereas only 40% of those regenerated using meristem culture were ymv-free. ymv-free yam plantlets could be propagated in vitro through n ... | 2013 | 23625083 |
| high genetic diversity, distant phylogenetic relationships and intraspecies recombination events among natural populations of yam mosaic virus: a contribution to understanding potyvirus evolution. | to evaluate the genetic diversity and understand the evolution of yam mosaic virus (ymv), a highly destructive pathogen of yam (dioscorea sp.), sequencing was carried out of the c-terminal part of the replicase (nib), the coat protein (cp) and the 3'-untranslated region (3'-utr) of 27 ymv isolates collected from the three main cultivated species (dioscorea alata, the complex dioscorea cayenensis-dioscorea rotundata and dioscorea trifida). ymv showed the most variable cp relative to eight other p ... | 2000 | 10640564 |
| the phylogeny of turnip mosaic virus; comparisons of 38 genomic sequences reveal a eurasian origin and a recent 'emergence' in east asia. | the genomes of a representative world-wide collection of 32 turnip mosaic virus (tumv) isolates were sequenced and these, together with six previously reported sequences, were analysed. at least one-fifth of the sequences were recombinant. in phylogenetic analyses, using genomic sequences of japanese yam mosaic virus as an outgroup, the tumv sequences that did not show clear recombination formed a monophyletic group with four well-supported lineages. these groupings correlated with differences i ... | 2003 | 12859632 |
| characterisation of a potyvirus and a potexvirus from chinese scallion. | molecular analyses of viruses infecting chinese scallion (allium chinense g. don) showed that the plants did not contain any of the poty-, carla- or allexiviruses that are common in garlic plants in china. the complete sequences of a potyvirus and a potexvirus were determined and these were shown to represent different viruses from any in the databases. they could be transmitted mechanically to scallion but not to other allium species (including garlic) or to narcissus. the potyvirus, tentativel ... | 2002 | 12038680 |
| rapid single-tube immunocapture rt-pcr for the detection of two yam potyviruses. | an immunocapture reverse transcription-polymerase chain reaction (ic-rt-pcr) based assay has been developed for the detection of yam-infecting potyviruses. based upon the same format two distinct simple tests have been developed, which allow the reliable diagnosis of yam mosaic virus and the tentatively named yam mild mosaic virus. by using immunocapture and a single-buffer rt-pcr reaction, the test can be performed in a single tube. the tests described have been shown to exhibit a thousand-fold ... | 1997 | 9504753 |
| determination of the taxonomic position and characterization of yam mosaic virus isolates based on sequence data of the 5'-terminal part of the coat protein cistron. | the sequences of the n-terminal part of the coat protein cistron from six isolates of yam mosaic virus (ymv-tog, ymv-cot, ymv-12, ymv-car, ymv-bu1 and ymv-bu2) were determined. the analysis of the deduced amino acid sequences revealed the presence of consensus motifs characteristic of the potyvirus genus supporting the classification of ymv as a potyvirus member. the alignment of the n-terminal part of the coat protein of ymv-tog, ymv-cot, ymv-12 and ymv-car showed that they were identical in si ... | 1996 | 8712924 |
| the use of phylogenetic data to develop molecular tools for the detection and genotyping of yam mosaic virus. potential application in molecular epidemiology. | molecular detection of yam mosaic virus (ymv), the most important potyvirus for yam plants (dioscorea spp.) has been limited by its high genetic diversity. according to recent phylogenetic data, suitable molecular tools were developed for detection and genotyping. a reliable and rapid molecular test is described using single-tube immunocapture (ic)-rt-pcr combining the use of a monoclonal antibody able to recognise all ymv serotypes and primers designed to take into account the whole variability ... | 2000 | 11011078 |
| rapid and specific detection of yam mosaic virus by reverse-transcription recombinase polymerase amplification. | yam mosaic virus (ymv; genus potyvirus) is considered to cause the most economically important viral disease of yams (dioscorea spp.) in west africa which is the dominant region for yam production globally. yams are a vegetatively propagated crop and the use of virus-free planting material forms an essential component of disease control. current serological and pcr-based diagnostic methods for ymv are time consuming involving a succession of target detection steps. in this study, a novel assay f ... | 2015 | 26115609 |
| complete nucleotide sequence of the genomic rna of a japanese yam mosaic virus, a new potyvirus in japan. | we determined the complete nucleotide sequence of a potyvirus purified from a japanese yam plant. the genomic rna of this virus is 9,757 nucleotides (nts) in length, excluding the 3'-terminal poly(a) tail. it contains a single open reading frame (orf) encoding a polyprotein of 3130 amino acids (aa) with a calculated mr of 356,793. the genomic organization of this potyvirus is similar to that of other members of the genus potyvirus and nine potential cleavage sites for the viral proteinase were f ... | 1999 | 10470250 |
| detection of japanese yam mosaic virus by rt-lamp. | arapid and simple procedure is described to detect the genomic rna molecule of japanese yam mosaic potyvirus (jymv). this method, named rt-lamp, allows direct detection of rna from infected plants without careful rna extraction, rapid thermal cycling and gel electrophoresis. rt-lamp was successfully applied to leaves, propagules and roots of japanese yam infected with jymv. one of the characteristics of the rt-lamp method is its ability to synthesize an extremely large amount of dna. accordingly ... | 2003 | 14505084 |
| complete genome sequence of a divergent strain of japanese yam mosaic virus from china. | a novel strain of japanese yam mosaic virus (jymv-cn) was identified in a yam plant with foliar mottle symptoms in china. the complete genomic sequence of jymv-cn was determined. its genomic sequence of 9701 nucleotides encodes a polyprotein of 3247 amino acids. its organization is virtually identical to that of two jymv isolates from japan. with the latter, it shares nucleotide sequence identities of only 74.7-74.8 %, indicating it might be a member of a new species. however, sequence analysis ... | 2015 | 25244960 |
| a genetic linkage map of guinea yam ( dioscorea rotundata poir.) based on aflp markers. | a genetic linkage map of the tetraploid white yam ( dioscorea rotundata poir.) was constructed based on 341 co-dominantly scored amplified fragment length polymorphism (aflp) markers segregating in an intraspecific f(1) cross. the f(1) mapping population was produced by crossing a landrace cultivar tdr 93-1 as female parent to a breeding line tdr 87/00211 as the male parent. the marker segregation data were split into maternal and paternal data sets, and separate genetic linkage maps were constr ... | 2002 | 12582485 |