evaluation of the stick enzyme immunoassay in caranx sp. and seriola dumerili associated with ciguatera.the stick enzyme immunoassay (s-eia) using monoclonal antibody to ciguatoxin (mab-ctx) was used to examine clinically implicated fish and to pre-screen two species of fish, caranx sp. (ulua or jack) and seriola dumerili (kahala or amberjack), supplied by sports fishermen. all of the clinically implicated fish from the department of health gave s-eia values greater than or equal to 1.3. the caranx sp. and seriola dumerili considered safe (less than or equal to 1.2 value) and consumed after testin ...19902231183
[risk factors of ciguatera in the french west indies in saint-barthélémy, saint-martin and anguilla].an epidemiological study on ciguatera fish poisoning in the french west indies (st-barthelemy, st-martin and anguilla) was conducted during the years 1985-1986. the investigation on intoxications shows a non seasonal significant prevalence. though it was difficult to list the cases, the morbidity seems to vary between 7 and 30 per thousand. a study of fish toxicity was realised; 46 different species were tested (individually or by pools) by the mosquito bio-test. observations of intoxications gi ...19892485547
fractionation and purification of some muscular and visceral ciguatoxins extracted from carribean fish.1. cigautoxins (ctx) were extracted from flesh and viscera of seven large roving predatory fishes: caranx bartholomaei, caranx latus, seriola dumerili, alectis crinitus, scomberomorus cavalla, sphyraena barracuda and gymnothorax funebris. 2. generally each extract consisted of close-related ctx which were separated according to their polarity by florisil column chromatography into a fast-acting ctx containing group and a slow-acting ctx containing group. 3. the shortest survival time of mice (ts ...19892620494
variations in symptomatology of ciguatera poisoning.ciguatera poisoning reports were examined for patterns of symptomatology when different types of fishes were consumed. consumption of surgeon fish (ctenochaetus strigosus), amberjack (seriola dumerili) and jack (caranx sp.) resulted in different symptom profiles with a number of statistically significant differences in the reported frequencies of specific symptoms. the results support the contention that the large variability in symptoms associated with ciguatera poisoning is caused by several c ...19892749758
[evaluation of different-stage levels of ciguatera toxicity of the marine food fish chain found around saint barthélemy island in french antilles].a total of 259 specimens of 32 potentially ciguatoxic fish species from st-barthelemy, leeward islands in the caribbean sea were checked for ciguatoxin. it was found that flesh and viscera from 229 specimens belonging to 27 species (essentially bottom-dwelling species) were ciguatoxic. no correlation between toxicity and species size or diet was demonstrated since five of the six highly ciguatoxic species seriola dumerili, caranx latus, caranx bartholomaei, caranx ruber and scomberomorus cavalla ...19863731371
urotensin ii-immunoreactive neurons in the caudal neurosecretory system of freshwater and seawater fish.antiserum generated against synthetic urotensin ii of the goby, gillichthys mirabilis, was used to localize urotensin ii in the caudal neurosecretory system in six species of freshwater teleosts: cyprinus carpio, carassius auratus, oreochromis mossambicus, oreochromis niloticus, salmo gairdneri and plecoglossus altivelis, and six species of seawater teleosts: acanthogobius flavimanus, pagrus major, parapristipoma trilineatum, trachurus japonicus, seriola dumerili and seriola quinqueradiata. in t ...19853884162
a rapid, simplified enzyme immunoassay stick test for the detection of ciguatoxin and related polyethers from fish tissues.a simplified and rapid enzyme immunoassay stick test is presented in this study. the salient feature of this test is the use of a coating (liquid paper) on the stick to adsorb the lipid ciguatoxin and its related polyether toxins onto the stick. this rapid solid phase stick test has been able to differentiate clinically implicated fishes (14 samples) from non-toxic (60 samples) with p less than 0.005. comparison of the stick test with the enzyme immunoassay procedure with the positive fish sampl ...19853913056
new staining methods for semithin sections of tissues of seriola dumerili embedded in methacrylate.semithin sections of different tissues of seriola dumerili r. embedded in glycol methacrylate, were stained with two staining methods normally used in conventional haematology and histology: gutierrez's cytopanchrome and gutierrez's polychrome v.o.f. the results observed in all tissues embedded in this resin and stained with v.o.f. are satisfactory and comparable, as to staining affinity, to those obtained on paraffin-embedded tissues. gutierrez's trichrome stain appears to be a useful tool in t ...19937693065
neobenedenia girellae (hargis, 1955) yamaguti, 1963 (monogenea: capsalidae) from cultured marine fishes of japan.the monogenean neobenedenia girellae (hargis, 1955) yamaguti, 1963 is redescribed and reported for the first time in japan. the parasite was recovered from the body surface, fins, and occasionally from the eyes of 14 species, comprising 5 families of cultured marine fishes from several localities in southwestern japan. neobenedenia melleni (maccallum, 1927) sensu kaneko et al. (1988) from tilapia (oreochromis mossambicus) in hawaii is synonymized with this species. examination of original specim ...19957707197
the adenohypophysis of mediterranean yellowtail, seriola dumerilii (risso, 1810): an immunocytochemical study.the adenohypophysis (adh) of the mediterranean yellowtail was studied using the peroxidase-antiperoxidase technique. human corticotropin (acth) (1-24)-immunoreactive (ir) cells were found bordering the neurohypophysis (nh) and salmon prolactin (prl)-ir cells were arranged in thick cords, both in the rostral pars distalis (rpd). gonadotropin (gth)-, thyrotropin (tsh)- and growth hormone (gh)-ir cells were observed in the proximal pars distalis (ppd). anti-chum salmon gth i and anti-chum salmon gt ...19968858883
importation of aquaculture seedlings to japan.japan is one of the largest consumers of fishery products in the world. importation of various fishery commodities - including larvae and eggs for aquaculture - is increasing due to high consumer demand. elvers of anguilla anguilla have been imported from europe since the late 1960s. eyed eggs of coho salmon (oncorhynchus kisutch) are imported from north america. during the last decade, over-production caused the price of japanese amberjack (or 'yellowtail') (seriola quinqueradiata) and japanese ...19968890372
immunocytochemical and ultrastructural characterization of prolactin, growth hormone, and somatolactin cells from the mediterranean yellowtail (seriola dumerilii, risso 1810).prolactin (prl), growth hormone (gh), and somatolactin (sl) are structurally related pituitary hormones that belong to a peptide family. whereas growth hormone and prolactin are present in the hypophysis of all vertebrates, somatolactin, a recently discovered hormone, has been found only in fish. it has been demonstrated immunocytochemically in a few teleost species; ultrastructurally, cells producing this hormone have been characterized only in one species of salmon. in this paper, we identify ...19979066917
identification of caribbean ciguatoxins as the cause of an outbreak of fish poisoning among u.s. soldiers in haiti.on 24 february 1995, six u.s. soldiers serving with the multinational force in haiti became ill after eating a locally caught fish identified as the greater amberjack seriola dumerili. the victims presented with nausea, vomiting, watery diarrhea and abdominal cramps 5-8 hr after consumption. also present in some victims were numbness in the extremities or perioral region, bradycardia and scalp paresthesia. patients were treated with i.v. hydration therapy and antiemetics. all recovered without s ...19979203298
isolation and characterization of two distinct gonadotropins from the pituitary gland of mediterranean yellowtail, seriola dumerilii (risso, 1810).two gonadotropins, gth i and gth ii, were isolated and chemically characterized from the pituitary of mediterranean yellowtail. they were extracted with 35% ethanol-10% ammonium acetate, separated by ion-exchange chromatography on a de-52 column, and purified by reversed-phase high-performance liquid chromatography on asahipak c4p-50 and subsequently by gel filtration chromatography on superdex 75. the molecular weights were estimated at 47 kda for gth i and 29 kda for gth ii by sds-page and at ...19979204373
immunocytochemical and ultrastructural characterization of melanotropin and adrenocorticotropin cells from the mediterranean yellowtail (seriola dumerilii, risso 1810).melanotropin (msh) and adrenocorticotropin (acth) are pituitary hormones derived from a common precursor: the proopiomelanocortin (pomc), which is processed differently in the melanotropic and corticotropic cells of several vertebrates. while acth is a major final product in corticotropes, it is further processed into alpha-msh and corticotropin-like intermediate lobe peptide (clip) in melanotropes. cells which are immunoreactive to acth (acth cells) and to both alpha-msh and acth (msh cells) ha ...19979294651
gonadotropic and thyrotropic cells from the mediterranean yellowtail (seriola dumerilii; risso, 1810): immunocytochemical and ultrastructural characterization.gonadotropins gth i and gth ii from the pituitary of mediterranean (m.) yellowtail (seriola dumerilii) were isolated and characterized, and antisera to the whole gth ii molecule (anti-my alpha,betagth ii) and to its beta-subunit (anti-my betagth ii) were obtained. at the light microscopic level, anti-my alpha,betagth ii reacted with my betagth ii-immunoreactive cells (gth ii cells), thyroid-stimulating hormone (tsh) cells, and a third cell population, which could have been gth i cells. the aim o ...19989566535
isolation and characterization of vibrio parahaemolyticus causing infection in iberian toothcarp aphanius iberus.high mortality among laboratory cultured iberian toothcarp aphanius iberus occurred in february 1997 in valencia (spain). the main signs of the disease were external haemorrhage and tail rot. bacteria isolated from internal organs of infected fish were biochemically homogeneous and identified as vibrio parahaemolyticus. the bacteria were haemolytic against erythrocytes from eel anguilla anguilla, amberjack seriola dumerili, toothcarp a. iberus and humans, and were kanagawa-phenomenon-negative. i ...199910073316
histochemical and morphometric aspects of the lateral musculature of different species of teleost marine fish of the percomorphi order.cross-sections of the lateral musculature of 19 species of teleost fish of the percomorphi order were studied histochemically and morphometrically. the red, pink and white muscles were observed in all the species except in seriola dumerilii and scomber japonicus, in which no pink muscle was found. the red fibres were the smallest due to their minimal diameters. histochemically and according to their location at least five types were found in the same species: typical red, in rosette, of the bord ...200011008368
investigation into the duality of gonadotropic cells of mediterranean yellowtail (seriola dumerilii, risso 1810): immunocytochemical and ultrastructural studies.two antisera against the follicle-stimulating hormone-like gonadotropin (fsh) of mediterranean (m.) yellowtail, anti-my fsha and anti-my fshb, were obtained. anti-my fsha serum specifically recognized fsh cells and did not react with any other pituitary cell type, while anti-my fshb serum recognized the alpha-subunit of the pituitary glycoprotein hormones and immunostained fsh, luteinizing hormone-like gonadotropin (lh), and thyrotropin (tsh) cells. anti-my fsha serum, together with a previously ...200212270785
numerical taxonomy of vibrionaceae isolated from cultured amberjack (seriola dumerili) and surrounding water.a numerical taxonomic study was performed on 148 isolates of gram-negative, heterotrophic, facultative anaerobic bacteria isolated from amberjack (seriola dumerili) and its surrounding culture water. the study included 30 type and reference strains belonging to genera vibrio, listonella, and photobacterium. the strains were characterized by 109 morphological, biochemical, physiological, and nutritional tests. cluster analysis of similarity matrices obtained with s(sm) and s(j) coefficients was c ...200312567241
occurrence of philometra lateolabracis (nematoda: philometridae) in the gonads of marine perciform fishes in the mediterranean region.gravid females of the nematode philometra lateolabracis (yamaguti, 1935), a parasite of gonads of marine perciform fishes, were found in wild and cultured dusky grouper epinephelus marginatus (lowe) from waters near the balear islands (spain, mediterranean sea) and sicily (italy, thyrrenean sea), and in the greater amberjack seriola dumerili (risso) in croatia (south-eastern adriatic sea). in wild e. marginatus in spain, the overall prevalence was 21% and the intensity of infection 1 nematode pe ...200312691199
girdles as the main infection site for paradeontacylix kampachi (sanguinicolidae) in the greater amberjack seriola this study, we provide new information about the habitats selected by the blood fluke paradeontacylix kampachi in the greater amberjack seriola dumerili based on an exhaustive anatomical examination. from may to october 1998, 21 fish of the 0+ age class were collected from tanks of the spanish institute of oceanography in puerto de mazarrón, spain, for parasitological analysis. individuals of p. kampachi were found in 17 of the 21 fish analysed (mean intensity +/- sd: 13.6 +/- 16.6; median: 6 ...200312691200
mucus ph of the tiger puffer takifugu rubripes is an important factor for host identification by the monogenean heterobothrium okamotoi.we examined a host-finding factor of the monogenean heterobothrium okamotoi oncomiracidia to develop an alternative prophylaxis. h. okamotoi oncomiracidia attached preferentially to gill filaments and skin mucus from the tiger puffer takifugu rubripes compared with corresponding material from other tested fishes (amber jack seriola dumerili, red sea bream pagrus major, japanese flounder paralichthys olivaceus and spotted halibut verasper variegatus). the body mucus ph of the tiger puffer was 6.4 ...200312964825
tormopsolus orientalis yamaguti, 1934 (digenea: acanthocolpidae) from seriola dumerili (risso) (perciformes: carangidae) in the western mediterranean sea.tormopsolus orientalis yamaguti, 1934, is redescribed from seriola dumerili from off corsica, majorca and aguilas, se spain. the vitellarium is interrupted at the level of the ovary and both testes, and a bipartite seminal vesicle is found in many specimens. oral sucker papillae are always seen. type-specimens and voucher specimens from off japan, bermuda, panama, curaçao and the great barrier reef have been compared with the mediterranean species. specimens of t. medius reimer, 1983, from mozam ...200415010594
four species of stephanostomum looss, 1899 (digenea: acanthocolpidae) from seriola dumerili (risso) (teleostei: carangidae) in the western mediterranean, including s. euzeti n. sp.four species of stephanostomum are described from various sites in seriola dumerili from off corsica, france. s. ditrematis (yamaguti, 1939), from the stomach, pyloric caeca and duodenum, has 36 circum-oral spines. s. seriolae yamaguti, 1970 is considered a synonym of s. ditrematis. s. filiforme linton, 1940, from the mid-intestine, has 43-46 circum-oral spines. s. petimba yamaguti, 1970, from the rectum, has a 42 circum-oral spines. s. euzeti n. sp. has 49-51 circum-oral spines, and differs fro ...200415084832
the ultrastructure of amberjack (seriola dumerilii) sperm.scanning and transmission electron microscopy were used to investigate the fine structure of sperm of the mediterranean amberjack seriola dumerilii. each spermatozoon has an ovoid head which lacks an acrosome, a short, irregularly-shaped midpiece and a long flagellar tail. the midpiece houses eight spherical mitochondria, which are separated from the axoneme by the cytoplasmic canal. the centrioles are arranged approximately at right angles to each other. the proximal centriole lies inside, and ...200215101444
lancefield group c streptococcus dysgalactiae infection responsible for fish mortalities in japan.a lancefield serological group c streptococcus sp. was isolated from cultured amberjack, seriola dumerili risso, and yellowtail, seriola quinqueradiata temminck and schlegel, immunized with lactococcus garvieae commercial vaccines in japan. the isolated bacteria were gram-positive cocci, auto-aggregating in saline, morphologically long chains in growth medium, catalase negative and alpha-haemolytic on blood agar. an almost complete gene sequence of the 16s rdna of two isolates was determined and ...200415575875
two species of the fish digenean genus rhipidocotyle diesing, 1858 (bucephalidae) reported for the first time from european seas.two species of rhipidocotyle are described from waters off the scandola nature reserve, corsica, france: these are the first records of these species from the mediterranean sea. r. capitata (linton, 1940) from auxis rochei and r. longicirrus (nagaty, 1937) n. comb. (originally bucephalopsis longicirrus nagaty, 1937) from seriola dumerili are also compared with similar species. r. capitata is similar to several other species of rhipidocotyle from scombrids, but differs in the structure of the rhy ...200516132870
three poorly known and rarely reported bucephalid species (digenea) in fishes from the western mediterranean.three poorly known bucephalid species are described from off corsica, in the western mediterranean sea. bucephalus gorgon (linton, 1905) (syn. b. introversus manter, 1940) from seriola dumerili is reported for only the third time in the mediterranean sea. prosorhynchoides arcuatus (linton, 1900) from sarda sarda is reported for the first time from the mediterranean sea. p. tergestinum (stossich, 1883) n. comb. (originally in gasterostomum von siebold 1848; has also been in bucephalopsis diesing, ...200516167122
characterization of lancefield group c streptococcus dysgalactiae isolated from farmed fish.a lancefield group c streptococcal (gcs) infection caused by streptococcus dysgalactiae that is characterized by severe necrotic lesions of the caudal peduncle has been an increasing cause of mortality in farmed fish such as amberjack, seriola dumerili, and yellowtail, seriola quinqueradiata, in the southern part of kyushu, japan. in this study, enzymatic profiles of gcs strains from fish and mammals were investigated using the api zym system, and genotypic characterization of gcs strains was pe ...200617169114
speciation of the paradeontacylix spp. (sanguinicolidae) of seriola dumerili. two new species of the genus paradeontacylix from the mediterranean.two new species of teleost blood fluke belonging to the sanguinicolid genus paradeontacylix are described from the greater amberjack, seriola dumerili, i.e. paradeontacylix ibericus n. sp. from the iberian peninsula and paradeontacylix balearicus n. sp. from the balearic islands. p. ibericus n. sp. and p. balearicus n. sp. show morphological similarities with paradeontacylix kampachi and paradeontacylix grandispinus respectively, which occur in mixed infection in s. dumerili from japan. multivar ...200818539079
lipid content and fatty acid composition of the monogenean neobenedenia girellae and comparison between the parasite and host fish species.neobenedenia girellae, a capsalid monogenean, is a destructive fish parasite. we studied the lipid content and fatty acid composition of n. girellae and the skin and cutaneous mucus of a host fish, the amberjack seriola dumerili (carangidae). the lipid content of adult n. girellae was less than one-fourth that of both the skin and cutaneous mucus of its host. adult n. girellae, s. dumerili skin and mucus had a relatively high weight-percentage of c16:0, c18:1(n-9), c18:0 and c22:6(n-3) fatty aci ...200818598577
detection of ciguatoxin in fish tissue using sandwich elisa and neuroblastoma cell bioassay.the applicability of a new enzyme-linked immunoassay (elisa) for detecting ciguatoxin (ctx) in fish tissue was evaluated by testing three fish species commonly implicated in ciguatera fish poisoning in hawaii. a total of 164 individual almaco jack (seriola rivoliana) and greater amberjack (s. dumerili) and a total of 175 individuals of the blue-spotted grouper (cephalopholis argus) were caught at various locations in the hawaiian islands. muscle tissue from each individual was assessed for the p ...200818623118
systematic status of philometra jordanoi (lópez-neyra, 1951) and some other congeneric species previously identified as philometra lateolabracis (yamaguti, 1935) (nematoda: philometridae).the systematic status of the gonad-infecting philometra species previously reported as p. lateolabracis (yamaguti, 1935) from marine fishes in the mediterranean region and off new caledonia is evaluated with respect to the recent redescription of p. lateolabracis from the type host in japan. philometra jordanoi (lópez-neyra, 1951) is revalidated to accommodate the nematodes from epinephelus marginatus, whereas the philometrids from other european hosts (mycteroperca rubra and seriola dumerili), ...200818666421
first report on antibody response of seriola dumerilii (risso 1810) challenged with listonella anguillarum.the greater amberjack, seriola dumerilii (risso 1810) is a semi-pelagic fish and a worldwide species; it is considered a promising candidate for the diversification of mediterranean aquaculture. in this paper an experimental injection with listonella (vibrio) anguillarum was performed to study the immune response of s. dumerilii. antibody titres to l. anguillarum o1 were determined with indirect-elisa at different times over a period of 42 days. results showed that the antibody levels against l. ...200818692575
a study of the lesions induced in seriola dumerili by intradermal or intraperitoneal injection of streptococcus of the species seriola dumerili were infected experimentally with the pathogen streptococcus dysgalactiae. intradermal (id) injection of s. dysgalactiae resulted in moderate mortality regardless of the dose of bacteria injected, whereas intraperitoneal (ip) injection caused greatest mortality in the group of fish receiving the highest dose of bacteria (10(9) colony forming units/ml). on necropsy examination of affected fish, the most striking change was microabscessation and/or granulomatou ...200919059602
[poisoning after ingestion of mediterranean greater amberjack liver: hypervitaminosis a clinical feature]. 201020537856
first toxin profile of ciguateric fish in madeira arquipelago (europe).ciguatera fish poisoning (cfp) is a human foodborne intoxication caused by ingestion of tropical fishes contaminated with the potent polyether toxins known as ciguatoxins (ctxs). these toxins are issued from gambierdiscus species of dinoflagellates. herbivorous fish accumulate these toxins in their musculature and viscera after ingesting dinoflagellates. epidemiological studies showed that cfp has been present in areas between 35 degrees north and 35 degrees south latitude, mainly, indo-pacific ...201020557036
lesions induced in seriola dumerili following exposure to streptococcus dysgalactiae by oral treatment or immersion.seriola dumerili were infected experimentally with streptococcus dysgalactiae by oral dosing or immersion. there was moderate mortality after immersion in water containing defined numbers of bacteria, regardless of the dose, whereas the effect on the oral challenge groups depended on the dose of bacteria administered. the characteristic lesions were microabscesses and/or pyogranulomatous inflammation of the caudal peduncle, pectoral and/or dorsal fin, heart and olfactory region. s. dysgalactiae ...201020570278
an evaluation of mercury levels in louisiana fish: trends and public health characterize statewide fish tissue mercury levels in edible finfish the first comprehensive analysis of louisiana's fish tissue mercury database was conducted. analyses were based on fifteen years of fish tissue mercury data collected from 368 waterbodies between 1994 and 2008 (n=14,344). the overall objectives of this study were to establish baseline fish tissue mercury levels; and evaluate species-specific temporal and spatial trends in fish tissue mercury levels. fish tissue mercury levels ...201020855108
vaccine efficacy of mycobacterium bovis bcg against mycobacterium sp. infection in amberjack seriola dumerili.mycobacteriosis, caused by the intracellular parasitism mycobacterium sp., causes economic damages to aquaculture production in japan, particularly in seriola fish production. antibiotics are not effective against mycobacterium sp. and so a potent vaccine is needed. we previously reported that bcg vaccine (mycobacterium bovis bcg) induces adaptive immunity against mycobacterium sp. in japanese flounder, paralichthys olivaceus. in a phylogenetic tree, the genes for a major antigen, the ag85 compl ...201021111051
a study of lesions induced in seriola dumerili infected naturally with streptococcus outbreak of disease in seriola dumerili occurred from august to october in 2007 and 2008. the fish developed lesions of the caudal peduncle, pectoral and/or dorsal fin and the heart. the lesions were characterized by moderate to severe infarction with areas of microabscessation and multifocal granulomatous inflammation associated with the presence of streptococcus dysgalactiae antigen. this is the first report to describe the immunohistology of the lesions induced in s. dumerili following nat ...201121453928
first report of gastrocotylinean post-oncomiracidia (platyhelminthes: monogenoidea: heteronchoinea) on gills of flyingfish (exocoetidae), snapper (lutjanidae), dolphinfish (coryphaenidae), and amberjack (carangidae) from the gulf of mexico: decoy hosts and the dilution effect.larvae, identified as post-oncomiracidia of the suborder gastrocotylinea (monogenoidea), were collected from formalin-fixed gills excised from six species of marine fishes captured from the gulf of mexico off mississippi and florida: common dolphinfish, coryphaena hippurus and pompano dolphinfish, coryphaena equiselis (both perciformes, coryphaenidae); gray snapper, lutjanus griseus (perciformes, lutjanidae); greater amberjack, seriola dumerili (perciformes, carangidae); and atlantic flyingfish, ...201121497672
Encephalomyelitis associated with microsporidian infection in farmed greater amberjack, Seriola dumerili (Risso).An outbreak of a disease characterized by a peculiar spiral movement in farmed greater amberjack, Seriola dumerili (Risso), occurred in Kagoshima Prefecture, Japan, in May 2008, immediately after importing the fish from China. Although neither bacteria nor viruses were detected in routine diagnostic tests, histopathological observations of the affected fish revealed severe inflammation in the tegmentum of the brain including the medulla oblongata and the anterior part of the spinal cord. In addi ...201122074018
seasonal population dynamics of zeuxapta seriolae (monogenea: heteraxinidae) parasitising seriola dumerili (carangidae) in the western mediterranean.we examined the seasonal and yearly population dynamics of the monogenean pathogen zeuxapta seriolae on juvenile fish from wild populations of seriola dumerili. the study is based on bimonthly monitoring between april, 2005 and april, 2007 off majorca, and newly obtained monogenean population data for juvenile fish from three additional localities in the western mediterranean (off alicante, corsica and sardinia). we documented the highest intensities and abundances of z. seriolae, with mean abun ...201223312866
effects of partial substitution of fish meal by soybean meal with or without heat-killed lactobacillus plantarum (lp20) on growth performance, digestibility, and immune response of amberjack, seriola dumerili juveniles.a 56-day feeding trial was conducted to evaluate the effects of supplemented diets with heat-killed lactobacillus plantarum (hk-lp) with graded levels of soybean meal (sbm) on growth, digestibility, blood parameters, and immune response of seriola dumerili (initial weight, 25.05 ± 0.1 g). seven isonitrogenous and isolipidic practical diets were formulated to contain 0%, 15%, 30%, and 45% sbm, and each sbm level was supplemented with hk-lp at 0.0 and 0.1%. fish fed diet which contains 30% sbm wit ...201525705667
identification and functional characterization of multiple interleukin 12 in amberjack (seriola dumerili).interleukin (il) -12 is a heterodimeric cytokine mainly produced by monocytes, macrophages, and dendritic cells in mammals. il-12p70 composed of il-12p35 and il-12p40, is known to play a crucial role in promoting cell-mediated immunity (cmi) through th1 differentiation and ifn-γ production. although two types of il-12p35 (p35a, p35b) and three types of il-12p40 (p40a, p40b and p40c) have been identified in several fish species, the knowledge on functional characteristics of teleost il-12 is stil ...201627238429
genetic characteristics of streptococcus dysgalactiae isolated from cage cultured cobia, rachycentron canadum (l.).disease outbreaks occurred during 2007-2013 in taiwan with 2.5-10% mortality among the cage cultured cobia, rachycentron canadum (l.), characterized by the presence of polyserositis, pericarditis and peritonitis. the micro-organisms isolated from internal organs were gram-positive cocci. the isolates were confirmed as streptococcus dysgalactiae by a polymerase chain reaction assay that yielded the expected specific 259 bp amplicon. additionally, partial sequence of the 16s-23s rdna intergenic sp ...201525087459
cloning and expression of a surface immunogenic protein in streptococcus dysgalactiae isolated from fish and its application in enzyme-linked immunosorbent assays to diagnose s. dysgalactiae infections in fish.lancefield group c streptococcus dysgalactiae (gcsd) causes severe necrotic lesions in the caudal peduncle in the genus seriola farmed in japan. to develop a sero-diagnostic method for gcsd infection in farmed fish, we attempted to identify a surface immunogenic protein that induces an antibody after infection with gcsd by immunoblot analysis using sera collected from infected fish. a protein obtained from sodium dodecyl sulfate (sds) extracts of gcsd was identified as s. dysgalactiae surface im ...201424131210
draft genome sequences of the fish pathogen vibrio harveyi strains vh2 and vh5.vibrio harveyi is an important marine pathogen that is responsible for vibriosis outbreaks in cultured fish and invertebrates worldwide. here, we announce the draft genome sequences of v. harveyi strains vh2 and vh5, isolated from farmed juvenile seriola dumerili during outbreaks of vibriosis in crete, greece.201526383670
draft genome sequence of vibrio parahaemolyticus vh3, isolated from an aquaculture environment in greece.vibrio parahaemolyticus is an important foodborne pathogen responsible for gastroenteritis outbreaks globally. it has also been identified as an important pathogen in aquatic organisms. here, we report a draft genome sequence of v. parahaemolyticus, strain vh3, isolated from farmed juvenile greater amberjack, seriola dumerili, in greece.201526139725
status and future perspectives of vaccines for industrialised fin-fish farming.fin fish farming is developing from extensive to intensive high industrial scale production. production of fish in high-density growth conditions requires effective vaccines in order to control persistent and emerging diseases. vaccines can also have significant positive impact on the reduced usage of antibiotics. this was demonstrated when vaccines were introduced in norway for atlantic salmon (salmo salar) in the late eighties and early nineties, resulting in a rapid decline of antibiotics con ...201323769873
three unicapsula species (myxosporea: trilosporidae) of asian marine fishes, including the description of unicapsula setoensis n. sp. in the yellowfin goby (acanthogobius flavimanus) from the inland sea of japan.the myxosporean genus unicapsula (multivalvulida: trilosporidae) is defined as having a spore with three unequal shell valves and polar capsules, of which one is prominent and the two other polar capsules are rudimentary. genetic characterization of members of the genus, currently 11 nominal species, is, at present, unsatisfactory yet when comparing to the closely related genus kudoa (multivalvulida: kudoidae). in the present study, we characterized long ribosomal rna gene (rdna) sequences of th ...201425088472
structural characterization of carangid fish myoglobins.the primary structures of myoglobin (mb) from the following five carangid species were determined: yellowtail seriola quinqueradiata, greater amberjack seriola dumerili, yellowtail kingfish seriola lalandi, japanese horse mackerel trachurus japonicus, and silver trevally pseudocaranx dentex. the sequences were of varying composition both in the coding and in the noncoding regions, but all contained the open reading frame of 444 nucleotides encoding 147 amino acids. amino acid sequence identities ...201222361749
massive consumption of gelatinous plankton by mediterranean apex predators.stable isotopes of carbon and nitrogen were used to test the hypothesis that stomach content analysis has systematically overlooked the consumption of gelatinous zooplankton by pelagic mesopredators and apex predators. the results strongly supported a major role of gelatinous plankton in the diet of bluefin tuna (thunnus thynnus), little tunny (euthynnus alletteratus), spearfish (tetrapturus belone) and swordfish (xiphias gladius). loggerhead sea turtles (caretta caretta) in the oceanic stage an ...201222470416
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