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[genetic distance of the malarial mosquitoes, anopheles beklemishevi and anopheles messeae (diptera, culiccidae), and their intraspecific polymorphism].allele frequencies at enzyme loci have been studied in finnish populations of two species of the anopheles maculipennis complex: a. beklemishevi and a. messeae. a. beklemishevi is spread over central and northern finland, whereas a. messeae is found in southwestern and central finland. the allele frequencies of these two species exhibit both similarities and differences. the results indicate that the two species do not interbreed in the nature. the allele frequencies at two loci--hydroxybutyrate ...1979510915
[inversion polymorphism in the malaria mosquito anopheles messeae. xi. the group effect of larval infection with bacillus thuringiensis subsp. israeliensis bacteria].genetic consequences of the group and individual infections of anopheles messeae larvae with bacteria bacillus thuringiensis subsp. israelensis (bti) were examined. the group effect of infections was manifested by increase in mortality and the speed-up of selection of chromosomal inversions resistant to bti. connection between the group and individual selections during the process of populations' adaptation is under discussion.19921427060
prehibernation diet and reproductive condition of female anopheles messeae in sweden.1. blood and sugar feeding in the mosquito anopheles messeae falleroni were investigated near uppsala, sweden, from 1981 to 1985, with emphasis on prehibernation diet. 2. fructose, indicating plant feeding, was present in females of all ovarian stages, from april to october, and contributed to fat body development in non-gonoactive females before hibernation. 3. an.messeae males and females (inseminated and uninseminated) were observed feeding on floral nectaries of the plants achillea millefoli ...19911768915
[inversion polymorphism in malaria mosquito anopheles messeae. part x. resistance of larvae with different genotypes to toxins of crystal-forming bacteria bacillus thuringiensis subsp. israelensis (serovar h14)].insensibility of larvae with different chromosomal inversions to the toxins of bacillus thuringiensis subsp. israelensis (bti) was examined. it has been shown that larvae with inversion combinations xl0(xl1)2r0-3r0-3l0 had greater variability after treatment with bti than larvae with inversions xl1(xl2)2r1-3r1-3l1(3l0). the former inversion combinations were previously shown to dominate in the south of the species area and to be supported by k-selection. the latter inversion combinations form "n ...19911874433
[experimental study of the routes of transmission of microsporidia in blood-sucking mosquitoes from southwestern siberia].experiments were conducted on the artificial infection of larvae of 8 species of bloodsucking mosquitoes (aedes diantaeus, a. punctor, a. cinereus, a. flavescens, a. aegypti, culex pipiens, anopheles messeae, anopheles atroparvus) with spores of amblyospora opacita var. sibirica, parathelohania costatus sp. n., p. obesa, p. anophelis. negative results were obtained in all experiments.19852864678
[ecology of anopheles messeae fall. in the zone influenced by the deep-water pre-dam area of the sayan water reservoir]. 19872960876
[changes in the population of anopheles messeae (fall, 1926) in the moscow district after discontinuation of ddt treatment (exemplified by the solnechnogorsk region)]. 19806448341
[cytogenetic and phenotypic variation in central and peripheral populations of the malaria mosquito, anopheles messeae fall. (diptera, culicidae)].chromosome polymorphism and morphological variation in malaria mosquito anopheles messeae larvae of populations from western siberia, kyrgyzstan, and southern kazakhstan were studied. it is shown that the level of inversion polymorphism decreases at the boundary of the range. a shift in the phenotypic norm observed in the peripheral biotopes is attributed to direct selection larvae with "southwestern" chromosomal variants and to modification.19969026461
[the season of possible malaria transmission by anopheles claviger and anopheles messeae mosquitoes in the chu river valley of the kyrgyz republic].the study was undertaken to define the limits of a possible season of malaria transmission by the prevalent species of anopheles mosquitoes in the chu valley situated in the north of the republic where large inhabited areas, including its capital bishkek, concentrated. the beginning of the malaria transmission season was considered to be associated with the actual emergence of potentially dangerous females whose physiological age at this time was equal or longer than the time required for sporog ...19979304029
[distribution of natural parasites--fungi and microsporidia--in a population of malarial mosquito larva (diptera: culicidae) before and after infection by the entomopathogenic bacteria bacillus thuringiensis israelensis].the distribution specificity of fungi and microsporidies in natural population of anopheles messeae fall. and a. beklemishevi stegny et kab. and those which survived after treatment them by bacillus thuringiensis israelensis (bti) were observed. parasitic fungus nonselectively affected individuals of both species and all inversion genotypes of a. messeae. microsporidia parathelohania messeae affected males and it has not species and genotypic specificity. the 4-th instar larvae of both species i ...19979395427
[sensitivity of larvae of the malaria mosquito anopheles messae with various inversion genotypes to the entomopathogenic bacteria bacillus thuringiensis israelensis as a function of dose].the influence of the entomopathogenic bacterium bacillus thuringiensis israelensis (bti) on the chromosomal composition of a natural population of fourth instar larvae of the malaria mosquito (anopheles messeae fall.) was investigated. the two doses used in the experiment yielded different death rates. a dose of 6.17 micrograms bti per 1 mg of host mass led to a death rate of 41.1 +/- 3.0%. the chromosomal composition of the population remained unchanged, but the proportion of xl11-2r11 females ...19979445817
[the relationship of the larval behavioral traits of the malarial mosquito anopheles messeae (diptera: culicidae) to its sensitivity to the entomopathogenic bacterium bacillus thuringiensis subspecies israelensis].behavioural actions of the malarial mosquito anopheles messeae larvae and their correlations with the susceptibility to the entomopathogenic bacterium bacillus thuringiensis israelensis (bti) were studied. the larvae were differentiated according to their feeding mode ("filter-feeders" and "scraper-feeders") and to their avoidance reaction on a concussion of water surface by water drops ("divers" and "undivers"). the "scraper-feeders" and "divers" showed a tendency of decreased susceptibility to ...19989612818
[the effect of infection by the entomopathogenic bacterium bacillus thuringiensis on the spread of microsporidia in an inversion-polymorphic population of the malarial mosquito anopheles messeae (diptera: culicidae)].a specificity of distribution of the microsporidia parathelohania sp. in a natural population of anopheles messeae and in those, which survived after the treatment by subspecies bacillus thuringiensis fukuokaensis, b. t. darmstadiensis and b. t. kyushuensis (pathotype b), were observed. the microsporidia parathelohania sp. affected males and it did not show a genotypic specificity in control, but it had a genotypic specificity after the b. thuringiensis subspecies treatment.19989702802
[the entomological and acarological situation in nizhegorod province].ixodes, persulcatus, i. ricinus, d. reticulatus, d. marginatus, h. concinna were recorded in different regional environmental regions. the northern districts of the region are endemic for tick-borne encephalitis (tbe) and ixodes tick-borne borreliosis (itbb). the infection of ticks with tbe virus is 0.25%, that with borrelia is 10.3% of the total number of the examinees. in 1997 tbe and ttbb morbidity rates were 1.91 and 2.0 per 100,000, respectively. data on the phenology of prevailing anophele ...199910414039
[refinement of the diagnosis of issia globifera (microsporidia: nosematidae) based on ultrastructural data].an ultrastructure of a microsporidian belonging to the aberrant and poorly studied genus issia is discribed in this paper. the first description of issia globulifera (issi, pankova, 1983) was made on the basis of light microscopic analysis of midgut smears of the infected insects. both late merogonic and sporogonic stages can be met on the sections from midguts of anopheles messae. meronts are ameba-like cells (3.0-3.5 x 2.0-2.5 microns) forming conglomerates of cells without any regular organiz ...200111548586
distribution and dynamics of larval populations of anopheles messeae and a. atroparvus in the delta of the rivers rhine and meuse, the netherlands.the distribution and ecology of mosquitoes of the anopheles maculipennis complex were studied in the delta of the rivers rhine and meuse in the southwest of the netherlands. the study area was previously malarious, with a. atroparvus being the only vector. 125 potential aquatic habitats of a. maculipennis were sampled, of which 47 (37.6%) contained larvae of this species complex. larval densities varied from 7.4-325.93 larvae m-2. there was no correlation between chlorinity (@1000) of the water ...200212164130
endemic malaria: an 'indoor' disease in northern europe. historical data analysed.endemic northern malaria reached 68 degrees n latitude in europe during the 19th century, where the summer mean temperature only irregularly exceeded 16 degrees c, the lower limit needed for sporogony of plasmodium vivax. because of the available historical material and little use of quinine, finland was suitable for an analysis of endemic malaria and temperature.200515847704
evaluation of potential west nile virus vectors in volgograd region, russia, 2003 (diptera: culicidae): species composition, bloodmeal host utilization, and virus infection rates of mosquitoes.potential west nile virus (family flaviviridae, genus flavivirus, wnv) vectors were assessed during 2003 at indoor and outdoor collection sites in urban volgograd, russia, and in three nearby towns and surrounding rural areas. in total, 9,182 female mosquitoes comprising 13 species in six genera were collected. relative abundance and bloodmeal host utilization differed temporarily and spatially. during june and july in volgograd, aedes vexans (meigen) (85.4%) and culex p. pipiens l. (7.6%) were ...200616739415
molecular identification of palearctic members of anopheles maculipennis in northern iran.members of anopheles maculipennis complex are effective malaria vectors in europe and the caspian sea region in northern iran, where malaria has been re-introduced since 1994. the current study has been designed in order to provide further evidence on the status of species composition and to identify more accurately the members of the maculipennis complex in northern iran.200717233887
how human practices have affected vector-borne diseases in the past: a study of malaria transmission in alpine valleys.malaria was endemic in the rhône-alpes area of eastern france in the 19th century and life expectancy was particularly shortened in alpine valleys. this study was designed to determine how the disease affected people in the area and to identify the factors influencing malaria transmission.200717727700
natural relapses in vivax malaria induced by anopheles mosquitoes.monthly malaria cases in finland during 1750-1850 revealed regionally different peaks. the main peak was in late spring in the whole country, but additional peaks occurred in august and december in some regions of finland. both primary infections and relapses caused deaths from malaria. the cause and timing of relapses are analysed.200818430203
dynamics of positional warfare malaria: finland and korea compared.a sudden outbreak of vivax malaria among finnish troops in se-finland and along the front line in hanko peninsula in the southwest occurred in 1941 during world war ii. the common explanation has been an invasion of infective anopheles mosquitoes from the russian troops crossing the front line between finland and soviet union. a revised explanation is presented based on recent studies of finnish malaria.200818778473
the decline of malaria in finland--the impact of the vector and social variables.malaria was prevalent in finland in the 18th century. it declined slowly without deliberate counter-measures and the last indigenous case was reported in 1954. in the present analysis of indigenous malaria in finland, an effort was made to construct a data set on annual malaria cases of maximum temporal length to be able to evaluate the significance of different factors assumed to affect malaria trends.200919422702
[population interactions of west nile virus (flaviviridae, flavivirus) with arthropode vectors, vertebrates, humans in the middle and low belts of volga delta in 2001-2006].the population interactions of west nile virus in the middle and lower regions of the volga delta in 2001-2006 were studied. the authors established major species of the mosquitoes anopheles messeae, an. hyrcanus, culex piplens, cx modestus, coquilletidia richiardii in anthropogenic biocenoses and an. hyrcanus, coq. richiardii in the natural ones; vertebrates - crows (corvidae) and domestic animals in the anthropogenic biocenoses and cormorants in the natural ones. the ticks hyalomma marginatum ...200919459411
assessing the future threat from vivax malaria in the united kingdom using two markedly different modelling approaches.the world is facing an increased threat from new and emerging diseases, and there is concern that climate change will expand areas suitable for transmission of vector borne diseases. the likelihood of vivax malaria returning to the uk was explored using two markedly different modelling approaches. first, a simple temperature-dependent, process-based model of malaria growth transmitted by anopheles atroparvus, the historical vector of malaria in the uk. second, a statistical model using logistic- ...201020205713
blood feeding patterns of mosquitoes: random or structured?abstract:201020205866
malaria risk in corsica, former hot spot of malaria in france.background: the prevalence of plasmodium falciparum and plasmodium vivax malaria was very high in corsica just before the second world war. the last outbreak was in 1972 and the most recent indigenous case was in 2006. results: analysis of historical data shows that anopheline vectors were abundant. recent surveys demonstrated that potential vectors are still present in corsica, despite the likely disappearance of anopheles sacharovi. moreover, p. falciparum can develop experimentally into these ...201020704707
the dominant anopheles vectors of human malaria in africa, europe and the middle east: occurrence data, distribution maps and bionomic précis.abstract:201021129198
why are anopheline mosquitoes not present in the seychelles?species of anopheline mosquitoes are largely distributed over emerged lands around the world and, within the tropics, few areas are without these insects, which are vectors of malaria parasites. among the exceptions is the seychelles archipelago in the western indian ocean. however, in the aldabra island group, located in the extreme western portion of the archipelago, anopheles gambiae s.l. was introduced, leading to massive proliferation and then elimination, with the most recent autochthonous ...201121303530
activation of the hypnozoite: a part of plasmodium vivax life cycle and survival.abstract:201121496287
variation in metabolic rate of anopheles gambiae and a. arabiensis in a sahelian village.in the sahel, the anopheles gambiae complex consists of anopheles arabiensis and the m and s molecular forms of a. gambiae sensu stricto. however, the composition of these malaria vectors varies spatially and temporally throughout the region and is thought to be linked to environmental factors such as rainfall, larval site characteristics and duration of the dry season. to examine possible physiological divergence between these taxa, we measured metabolic rates of mosquitoes during the wet seaso ...201121697426
[inversion polymorphism of the malaria mosquito anopheles messeae. viii. distribution of larvae in biotopes under conditions of co-habitation with anopheles beklemishevi].the role of chromosomal variability was studied in the process of biotopical distribution of larvae of the sibling species anopheles messeae and a. beklemishevi. it is shown that frequency of the inversions xl1, xl2, 2r1, 3r1 of a. messeae was changed in the lakes with different larval quantity of the sibling species. mosquito of a. beklemishevi were developed in the suboptimal biotops having low larwal quantity of a. messeae with the analogous chromosomal arrangement 2r1. the larvae with "north ...20092737465
nationwide inventory of mosquito biodiversity (diptera: culicidae) in belgium, europe.to advance our restricted knowledge on mosquito biodiversity and distribution in belgium, a national inventory started in 2007 (modirisk) based on a random selection of 936 collection points in three main environmental types: urban, rural and natural areas. additionally, 64 sites were selected because of the risk of importing a vector or pathogen in these sites. each site was sampled once between may and october 2007 and once in 2008 using mosquito magnet liberty plus traps. diversity in pre-def ...201222971463
current status of invasive mosquito surveillance in the uk.non-native invasive mosquitoes have for many years made incursions into europe, and are now established in many european countries. the continued european importation of potential vectors and their expansion within europe increases their potential for importation and establishment in the uk. coupled with increasing numbers of returning dengue and chikungunya infected travellers, the potential exists for transmission of vector borne disease in new regions.201526122427
pilot longitudinal mosquito surveillance study in the danube delta biosphere reserve and the first reports of anopheles algeriensis theobald, 1903 and aedes hungaricus mihályi, 1955 for romania.mosquito-borne viruses (moboviruses) are of growing importance in many countries of europe. in romania and especially in the danube delta biosphere reserve (ddbr), mosquito and mobovirus surveillance are not performed on a regular basis. however, this type of study is crucially needed to evaluate the risk of pathogen transmission, to understand the ecology of emerging moboviruses, or to plan vector control programmes.201627066827
direct pcr of indigenous and invasive mosquito species: a time- and cost-effective technique of mosquito barcoding.millions of people die each year as a result of pathogens transmitted by mosquitoes. however, the morphological identification of mosquito species can be difficult even for experts. the identification of morphologically indistinguishable species, such as members of the anopheles maculipennis complex (diptera: culicidae), and possible hybrids, such as culex pipiens pipiens/culex pipiens molestus (diptera: culicidae), presents a major problem. in addition, the detection and discrimination of newly ...201526663040
impacts of the creation, expansion and management of english wetlands on mosquito presence and abundance - developing strategies for future disease mitigation.the incidence of mosquito-borne diseases is increasing in europe, partly due to the incursion of a number of invasive species known to be vectors of dengue and chikungunya viruses, but also due to the involvement of native species in the transmission of west nile virus and malaria. for some of these pathogens, there is a risk of the re-emergence of vector-borne diseases that were once widespread in europe, but declined partly due to large-scale land-drainage projects. some mosquito species explo ...201525889666
west nile virus state of the art report of malwest project.during the last three years greece is experiencing the emergence of west nile virus (wnv) epidemics. within this framework, an integrated surveillance and control programme (malwest project) with thirteen associate partners was launched aiming to investigate the disease and suggest appropriate interventions. one out of seven work packages of the project is dedicated to the state of the art report for wnv. three expert working groups on humans, animals and mosquitoes were established. medical dat ...201324317379
monitoring of west nile virus in mosquitoes between 2011-2012 in hungary.west nile virus (wnv) is a widely distributed mosquito-borne flavivirus. wnv strains are classified into several genetic lineages on the basis of phylogenetic differences. whereas lineage 1 viruses are distributed worldwide, lineage 2 wnv was first detected outside of africa in hungary in 2004. since then, wnv-associated disease and mortality in animal and human hosts have been documented periodically in hungary. after the first detection of wnv from a pool of culex pipiens mosquitoes in 2010, s ...025229703
molecular confirmation of the occurrence in germany of anopheles daciae (diptera, culicidae).anopheles daciae, a newly described member of the maculipennis group, was recently reported from western, southern and eastern europe. before its recognition, it had commonly been listed under the name of an. messeae, due to its extreme morphological and genetic similarities. as the sibling species of the maculipennis group are known to differ in their vector competences for malaria parasites and other pathogens, the occurrence of an. daciae in a given region might have an impact on the epidemio ...201223146352
anopheles plumbeus (diptera: culicidae) in europe: a mere nuisance mosquito or potential malaria vector?anopheles plumbeus has been recognized as a minor vector for human malaria in europe since the beginning of the 20th century. in recent years this tree hole breeding mosquito species appears to have exploited novel breeding sites, including large and organically rich man-made containers, with consequently larger mosquito populations in close vicinity to humans. this lead to investigate whether current populations of an. plumbeus would be able to efficiently transmit plasmodium falciparum, the pa ...201223181931
ecophysiology of anopheles gambiae s.l.: persistence in the sahel.the dry-season biology of malaria vectors is poorly understood, especially in arid environments when no surface waters are available for several months, such as during the dry season in the sahel. here we reappraise results on the dry-season physiology of members of the anopheles gambiae s.l. complex in the broad context of dormancy in insects and especially in mosquitoes. we examine evidence on seasonal changes in reproduction, metabolism, stress tolerance, nutrition, molecular regulation, and ...201424933461
effects of microclimate condition changes due to land use and land cover changes on the survivorship of malaria vectors in china-myanmar border region.in the past decade, developing countries have been experiencing rapid land use and land cover changes, including deforestation and cultivation of previously forested land. however, little is known about the impact of deforestation and land-use changes on the life history of malaria vectors and their effects on malaria transmission. this study examined the effects of deforestation and crop cultivation on the adult survivorship of major malaria mosquitoes, anopheles sinensis and an. minimus in the ...201627171475
diversification of the genus anopheles and a neotropical clade from the late cretaceous.the anopheles genus is a member of the culicidae family and consists of approximately 460 recognized species. the genus is composed of 7 subgenera with diverse geographical distributions. despite its huge medical importance, a consensus has not been reached on the phylogenetic relationships among anopheles subgenera. we assembled a comprehensive dataset comprising the coi, coii and 5.8s rrna genes and used maximum likelihood and bayesian inference to estimate the phylogeny and divergence times o ...201526244561
the ecology and larval habitats characteristics of anopheline mosquitoes (diptera: culicidae) in aligudarz county (luristan province, western iran).to determine ecology and characteristics of the larval habitats of the genus anopheles (dipetra: culicidae) in aligudarz county, western iran.025183088
molecular species identification, host preference and detection of myxoma virus in the anopheles maculipennis complex (diptera: culicidae) in southern england, uk.determining the host feeding patterns of mosquitoes by identifying the origin of their blood-meals is an important part of understanding the role of vector species in current and future disease transmission cycles. collecting large numbers of blood-fed mosquitoes from the field is difficult, therefore it is important to maximise the information obtained from each specimen. this study aimed to use mosquito genome sequence to identify the species within anopheles maculipennis sensu lato (an. macul ...201526271277
analysis of the causes of spawning of large-scale, severe malarial epidemics and their rapid total extinction in western provence, historically a highly endemic region of france (1745-1850).the two main puzzles of this study are the onset and then sudden stopping of severe epidemics in western provence (a highly malaria-endemic region of mediterranean france) without any deliberate counter-measures and in the absence of significant population flux.201424581282
imported malaria in albania and the risk factors that could allow its reappearance.malaria is an infectious disease gradually becoming a serious concern for public health institutions, even in european countries where the eradication of the disease was previously taken for granted. albania was listed as an endemic area from the beginning of the 20th century, but the disease was gradually under control and some decades after the world war ii it was merely considered a historical curiosity. nevertheless, for many reasons, since 1994 and in increasing trend, albanian health facil ...201323758911
expansion of anopheles maculipennis s.s. (diptera: culicidae) to northeastern europe and northwestern asia: causes and consequences.the burden of malaria infection in the modern world remains significant. specific changes in the relative proportions of malaria vector mosquitoes, maculipennis complex species, in the south of western siberia over the past 25 years of the 20th century have attracted wide attention as an indicator of their dynamic geographical distribution. in eurasia, studies of fluctuations in the borders of areas occupied by sibling species of this complex, as well as their relative proportions in the areas w ...201425148893
climate change is increasing the risk of the reemergence of malaria in romania.the climatic modifications lead to global warming; favouring the risk of the appearance and development of diseases are considered until now tropical diseases. another important factor is the workers' immigration, the economic crisis favouring the passive transmission of new species of culicidae from different areas. malaria is the disease with the widest distribution in the globe. millions of people are infected every year in africa, india, south-east asia, middle east, and central and south am ...201627847824
tissue-specific differences in the spatial interposition of x-chromosome and 3r chromosome regions in the malaria mosquito anopheles messeae fall.spatial organization of a chromosome in a nucleus is very important in biology but many aspects of it are still generally unresolved. we focused on tissue-specific features of chromosome architecture in closely related malaria mosquitoes, which have essential inter-specific differences in polytene chromosome attachments in nurse cells. we showed that the region responsible for x-chromosome attachment interacts with nuclear lamina stronger in nurse cells, then in salivary glands cells in anophele ...201525671311
genetic diversity of two daphnia-infecting microsporidian parasites, based on sequence variation in the internal transcribed spacer region.microsporidia are spore-forming obligate intracellular parasites that include both emerging pathogens and economically important disease agents. however, little is known about the genetic diversity of microsporidia. here, we investigated patterns of geographic population structure, intraspecific genetic variation, and recombination in two microsporidian taxa that commonly infect cladocerans of the daphnia longispina complex in central europe. taken together, this information helps elucidate the ...201627206473
the first finnish malariologist, johan haartman, and the discussion about malaria in 18th century turku, finland.after the great northern war in 1721, sweden ceased to be an important military power. instead, the kingdom concentrated on developing science. swedish research got international fame with names as carolus linnaeus, pehr wargentin and anders celsius. medical research remained limited and malaria was common especially in the coastal area and along the shores of the big lakes.already in the beginning of the 18th century swedish physicians recommended peruvian bark as medication and they also empha ...201121324104
[septicaemia of chironomid larvae (diptera: chironomidae) promoted by bacillus cereus and b. thuringiensis].natural factors regulating the population of chironomids were studied. the bacteria bacillus cereus were isolated from chironomids sampled from kuyalnitskii firth after epizooty of chironomus sp., and bacteria bacillus thuringiensis spp. israelensis (bti) were isolated from dead larva of chironomus plumosus sampled in the sea of azov (3-m depth). bti were characterized by high insecticide activity on midges anopheles messeae fall., aedes cireneus mg., and culex pipiens pipiens f. pipiens l.201622988756
pcr identification and distribution of anopheles daciae (diptera, culicidae) in germany.based primarily on nucleotide polymorphisms in the internal transcribed spacer 2 (its2) of the ribosomal dna, anopheles daciae was recently described as an additional member of the maculipennis group of species, separate from anopheles messeae with which it had previously been confused due to morphological and genetic similarity. species differentiation between a. messeae and a. daciae was possible only by its2 polymerase chain reaction (pcr) amplification followed by dna sequencing or rflp anal ...201424696275
detection of theileria orientalis in mosquito blood meals in the united kingdom.theileria spp. are tick-borne protozoan parasites that infect a wide range of wild and domestic animals. in this study, the utility of xenosurveillance of blood-fed specimens of culiseta annulata for detecting the presence of piroplasms in livestock was investigated. blood-fed mosquitoes were collected at elmley national nature reserve, kent, united kingdom. all specimens were morphologically identified, and dna barcoding was used to confirm the morphological identification. both the vertebrate ...201627809975
comparison of fatty acid contents and composition in major lipid classes of larvae and adults of mosquitoes (diptera: culicidae) from a steppe region.emerging aquatic insects, including mosquitoes, are known to transfer to terrestrial ecosystems specific essential biochemicals, such as polyunsaturated fatty acids (pufa). we studied fatty acid (fa) composition and contents of dominant mosquito populations (diptera: culicidae), that is, anopheles messeae, ochlerotatus caspius, oc. flavescens, oc. euedes, oc. subdiversus, oc. cataphylla, and aedes cinereus, inhabited a steppe wetland of a temperate climate zone to fill up the gap in their lipid ...201323956110
the anopheles maculipennis complex (diptera: culicidae) in germany: an update following recent monitoring activities.the anopheles maculipennis complex comprises several sibling species including major vectors of malaria parasites of historic europe. in present-day europe, these species are probably more relevant with regard to transmission of pathogens other than plasmodia, such as viruses and dirofilariae. distribution data facilitating risk assessments and modelling of an. maculipennis complex-borne diseases, however, are generally outdated. in germany, the occurrence and geographic distribution of the comp ...201627444437
occurrence and host preferences of anopheles maculipennis group mosquitoes in england and wales.mosquitoes of the anopheles maculipennis meigen (diptera: culicidae) group are of public health concern: five of the 11 morphologically indistinct species have been historically considered as vectors of malaria in europe. three members of the an. maculipennis group have been reported in the u.k.: anopheles atroparvus van thiel; anopheles messeae falleroni, and anopheles daciae linton, nicolescu & harbach. to study the distribution of the three u.k. species, particularly that of an. daciae, we de ...201423848304
identification of anopheles daciae in germany through its2 sequencing.until the middle of the twentieth century, malaria was frequently endemic in parts of germany; anopheles maculipennis complex species were considered the primary vectors. three species of this complex have been identified in germany: a. maculipennis s.s., anopheles messeae and anopheles atroparvus; the last predominantly from the coastal regions of northern germany. anopheles daciae is a recently described member of the a . maculipennis complex and resembles the well-characterised species a. mes ...201223001548
[escape reactions and variation in some genetic parameters in larva from the malaria mosquito anopheles messeae].the relationship between the escape response and four types of variations--inversion polymorphism, the number of denses in the stigmal plate ridges, their asymmetry, and stripe-nonstripe variation--was studied in larvae of malaria mosquito anopheles messeae fall. from natural populations. pigmented individuals were shown to have a more reduced responce than nonpigmented ones. asymmetry declined in the direction from unresponsive larvae to those repeatedly (responded). number of denses was lower ...19989719922
intragenomic heterogeneity of rdna internal transcribed spacer 2 in anopheles messeae (diptera: culicidae).because anopheles messeae falleroni (diptera: culicidae) is one of the main vectors of malaria in russia, studying its genetic markers is important for reliable identification of this species. this species is distributed nearly throughout the palearctic region, and it exhibits high genetic variability. we investigated polymorphism of the rdna internal transcribed spacer (its) 2 of an. messeae in various regions of russia, and we found intragenomic heterogeneity of its2 copies verified by chromat ...200818533424
intrapopulation polymorphism in anopheles messeae (an. maculipennis complex) inferred by molecular analysis.we evaluated the internal transcribed spacer two (its2) sequence to detect intraspecific polymorphism in the palearctic anopheles maculipennis complex, analyzing 52 populations from 12 countries and representing six species. for an. messene, two fragments of the cytochrome oxidase i (coi) gene were also evaluated. the results were compared with genbank sequences and data from the literature. its2 analysis revealed evident intraspecific polymorphism for an. messeae and a slightly less evident pol ...200415311447
[systemic reorganization of the architectonics of polytene chromosomes in the onto- and phylogenesis of malaria mosquitoes].cardinal differences in the spatial organization of polytene chromosomes in the nuclei of salivary gland cells (malphigian tubules) and nurse ovarian cells in malarial mosquitoes of anopheles maculipennis complex are found. the chromosomes have typical chromocentric organization in salivary gland cells and malphigian tubules. in contrast to these cells, the trophocytes have no single chromocentre and some of their chromosomes are rigidly attached to the nucleus envelope with the centromeric part ...19873623084
[comparative analysis of dna sequences of regions of x-chromosome attachment to the nuclear envelope of nurse cells anopheles messeae fall].polytene chromosomes of ovarian nurse cells of anopheles mosquitoes form strong contacts with the nuclear envelope. the presence of contacts, their position at nurse cell chromosomes, and their morphological features are species-specific in malaria mosquitoes. it is important to determine the nature of these interspecies differences in the nuclear architecture, both to understand the function of the nucleus and to assess the role of the spatial organization of chromosomes in evolution. using dot ...201526410936
frequency tuning of individual auditory receptors in female mosquitoes (diptera, culicidae).the acoustic sensory organs in mosquitoes (johnston organs) have been thoroughly studied; yet, to date, no data are available on the individual tuning properties of the numerous receptors that convert sound-induced vibrations into electrical signals. all previous measurements of frequency tuning in mosquitoes have been based on the acoustically evoked field potentials recorded from the entire johnston organ. here, we present evidence that individual receptors have various frequency tunings and t ...201323742968
[chromosomal polymorphism in the populations of malaria mosquito anopheles messeae (diptera, culicidae) at the south of russian plain].zonal features of the geographic distribution of chromosomal inversions in the populations of an. messae at the south of the russian plain were examined. the an. messae was identified based on morphological characters and cytogenetically. the chromosomal inversions identified in populations of an. messae comprised xl1, xl4, 2r1, 3r1, and 3l1. inversion xl4 was endemic and found with low frequency (2%) in penza oblast and moscow oblast. based on the population karyotype structure similarity, on t ...201223113341
[inversion polymorphism and ecological specialization of malaria mosquitoes (diptera, culicidae) in karelia].the species composition and inversion polymorphism were studied in malaria mosquito larva hemipopulations of karelia. three malaria mosquito species--anopheles messeae, a. beklemishevi, and a. maculipennis--were found in the region. the northern boundary of their range is at 65 degrees nl. the greatest species diversity was observed in biotopes of the central region. within-species chromosome polymorphism was observed in larva populations of all three species. for a. messeae, maximum karyotype d ...201222988766
[molecular genetic analysis of the x-chromosomal nuclear envelope attachment region in nurse cells of the malaria mosquitoes anopheles messeae fall].dna of the x-chromosomal nuclear envelope attachment region was isolated from malaria mosquito anopheles messeae fall. nurse cells by chromosome microdissection. a dna library of the region was constructed using a plasmid vector. dna sequencing revealed gene fragments, tandem repeats, and a great variety of transposable elements (tes). the x-chromosomal nuclear envelope attachment region was concluded to correspond in molecular organization and cytogenetics to diffuse intercalary heterochromatin ...201122232918
[inversion polymorphism in malarial mosquito anopheles messeae natural populations from the north-eastern region of the range].a cytogenetic study of eight natural populations of anopheles messeae from the north-eastern part of the species areal was conducted. complete predominance of homozygotes xl11 and 3r11 in the northern populations was observed. change in the chromosome 2 inversion frequencies from south northwards was shown. the 2r11 variant, which was not observed in the southern region, was found in the northern populations. these results indicate the maintenance of chromosome frequency of the distribution of i ...201021254547
[genetic determination of succinate dehydrogenase activity in anopheles messeae (diptera, culicidae) larvae].the present study aimed to reveal differences in the activity of a mitochondrial enzyme, succinate dehydrogenase (sdg), in larvae of mosquito anopheles messeae with various karyotypes. four-instar larvae of malaria mosquito previously obtained in laboratory conditions from imagoes collected in a taiga population of tomsk region served as material for the study.200919947542
[chromosome polymorphism and regularities of the subpopulation organization of malaria mosquitoes anopheles (diptera, culicidae) in biotopes of the tomsk oblast].a biotopic subdivision was observed for the two closely related species anopheles messeae and a. beklemishevi in larval biotopes of the tomsk oblast. the regularities of the spatial distribution of a. messeae with various chromosomal inversions were determined. the a. messeae karyotypic structure proved to vary depending on the ecological conditions of wintering and reproduction sites. the frequencies of chromosome variants xl0, 2r0, 3r0, and 3l0 were maximal in villages, while forest biotopes w ...200919507700
[ecology and epizootology of microsporidia in malarial mosquitoes (diptera: culicidae) from the south of western siberia].the ecology and epizootology of microsporidia were studied in the natural population of malarial mosquitoes from western siberia over a 30-year period. symptoms of the disease, host specificity and character of parasite localization in host tissues were investigated. microsporidia of 9 species from 4 genera, namely amblyospora, crepidulospora, senoma, and parathelohania, were found in the malarial mosquito larvae from the territory examined. the mosquito species anopheles messeae was infested by ...200818664069
[inversion polymorphism and the divergence of two cryptic forms of anopheles messeae (diptera, culicidae) at the level of genomic dna repeats].genomic dna of samples from several populations of malaria mosquito anopheles messeae belonging to the macullipennis paleoarctic complex was examined using digestion with ten restriction enzymes and electrophoresis in polyacrylamide gel. the patterns of dna repeat fraction in the ecorii hydrolysate in two cryptic forms of an. messeae characterized by common inversion polymorphism were shown to be different. the genomic differences in mixed sympatric groups and those between geographically remote ...200111558231
[pericentromeric and intercalating alpha-heterochromatin of polytene chromosomes of the malaria mosquito].the properties of heterochromatin from polytene chromosomes of the malaria mosquito anopheles messeae fal. and a. atroparvus v. tiel. were studied by various methods of differential staining and by hybridization in situ with two repetitive dna sequences of drosophila melanogaster. in malaria mosquito, the heterochromatin was heterogeneous. two forms of alpha-heterochromatin were revealed: pericentromeric and intercalary heterochromatin, which is localized within the internal chromosome regions.200010752029
[interaction between three types of variation and fluctuating asymmetry in the population of malaria mosquito anopheles messeae fall].in the population of the malaria mosquito anopheles messeae, interrelations of three types of variation (polymorphism for inversions, dorsal color of abdomen and thorax, and number of dents in the crests of the stigmal plate) and fluctuating asymmetry were studied. the presence of white pigmentation on the larval dorsum did not correlate with inversion polymorphism and fluctuating asymmetry, but correlated with the number of small and large dents in the crests of stigmal plate. asymmetry was sho ...19989929828
[kin selection and developmental rate of malarial mosquitoes with different karyotypes].changes in the adaptive value of genotypes through the course of relationships within and between malaria mosquito families at larval stages were studied in anopheles messeae, the species with chromosome polymorphism. it was shown that interactions between sibs can affect their viability and developmental rate. larvae with "northern" inversions (r-strategists) developed significantly slower when kept together with larvae carrying the "southern" chromosome variants (k-strategists). a decrease in ...19979445801
[polymorphism of pericentromere heterochromatin of polytene chromosomes of ovarian trophocytes in natural populations of the malaria mosquito anopheles messeae fall].pericentric heterochromatin polymorphism in chromosome 2 of ovarian trophocytes was studied in three natural populations of the malaria mosquito anopheles messeae fall. it is shown that pericentric heterochromatin blocks occur in four different variants whose sizes are at the 1:2:3:4 ratio (single, double, triple, and quadruple blocks). mosquitoes with homo- and heterozygous variants of these blocks were identified. by analyzing frequency distribution of different size variants, interpopulation ...19979162707
[chromocenter organization in salivary glands of the malaria mosquito anopheles messeae fall].the chromocenter organization and the relationship with the nuclear envelope were studied in anopheles messeae fall. malaria mosquito. the interchromosomal contacts within the chromocenter were shown to be formed by both alpha- and beta-heterochromatin. in the pericentric regions of all chromosomes and in highly repeated sequences localized mostly in alpha-heterochromatin, the incorporation of the moderate he-t repeat of d. melanogaster was also found. each chromosome was shown to have an indepe ...19979162696
[spatial organization of chromosomes in oocytes and spermatocytes in malaria mosquitos].it is shown that prophase chromosomes of oocytes in anopheles messeae ovaries do not form local chromocenters, unlike spermatocytes, in which chromosomes fuse in a joint centromeric assembly. this fact reflects the dynamic nature of the system of chromocenter formation in generative tissues. during analysis of interspecific hybrids f1 a. maculipennis x a. subalpinus, no conjugation of homeologous chromosomes was observed, and the latter remained separated from one another.19957721071
[strategies for selection and stability to asphyxia in larvae of the malaria mosquito anopheles messeae with various karyotypes].sensitivity to asphyxia (oxygen deficiency) anopheles messeae larvae was studied. it was demonstrated that mosquitoes with mosquitoes with "northern" chromosome inversions perish underwater more more quickly than mosquitoes with "southern" karyotypes. apparently, resistance to asphyxia is a component of the adaptive strategy and is maintained by k-selection. the respiration and energy metabolism of larvae with different gene complexes are discussed.19957721058
[the morphofunctional changes in the ovarioles of blood-sucking mosquitoes (diptera, culicidae) during oogenesis. 3. anomalous forms of the ovarioles].the authors describe blood-sucking mosquito ovarioles in which the development of germarium or follicles is abnormal and asynchronous with the gonotrophic cycles. the following morphologic variants of abnormal ovarioles may be distinguished: club-shaped without follicles; multichamber with several follicles; miniature with a diminished follicle; with atypical oocyte number in a follicle. the ovariole sterility may be due to cessation of germarium functioning (club-shaped ovarioles consisting of ...20098028566
[systemic reorganization of the architechtonics of polytene chromosomes in onto- and phylogenesis of malaria mosquitoes. structural features regional of chromosomal adhesion to the nuclear membrane].principles of organization of chromocenter in salivary gland cells and zones of chromosome attachment to nuclear envelope in ovarian nurse cells were determined. it was shown that blocks of centromeric heterochromatin (alfa-heterochromatin) have no direct connection with nuclear envelope. such connections are ensured by beta-heterochromatin. homologous chromosome regions were shown to be of different morphology and nature of chromosome-membrane links in different mosquito species. a map of polyt ...19911916252
[the microsporidian parathelohania illinoisensis var. messeae (amblyoporidae) from the mosquito anopheles messeae in the tom-ob river area]. 20071813844
[inversion polymorphism of the malaria mosquito anopheles messeae. ix. cannibalism in larvae as a selection factor].the genetic consequences of larval cannibalism were studied in mosquito anopheles messeae fall. (diptera. culicidae) with "south" inversional combinations. it is shown that mosquito with karyotypes xl11(2)r00(3)r00(01, 11) eat the larvae of the species named more actively than mosquito with karyotypes xl00(01)2r00(3)r00(01)3l00(01, 11). evidently, adaptation of mosquito with "south" genocomplexes was developed in two directions: 1) more effective utilization of food resources in the larvae with ...19902079206
[distribution of anopheles messeae fall. in the sayan reservoir during its filling. the ecological characteristics of this species]. 20093367869
[inversion polymorphism in the malaria mosquito anopheles messeae. vii. fertility and the population genetics structure of the species].the role of chromosomal inversional complexes of different fecundity has been studied in the process of formation of karyotypic populational structure. females with homozygotes 2r11 and "north-est" inversional combinations are shown to have maximal total egg production, while females with three or four inversional heterozygotes from xl01, xl12, 2r01, 3r01 and 3l01 demonstrated lower level of fecundity. it is found that the seasonal conversion from r-selection to k-selection occurs in the period ...19873440514
[appearance of a population of anopheles messeae mosquitoes downstream of the sayan-shushenskoe reservoir]. 20093960003
[discovery of anopheles messeae fall. breeding sites at the sayan reservoir and in the tailrace of the yenisei in the construction area of sayan-shushenskoe hydroelectric station]. 20093959992
[inversion polymorphism in the malaria mosquito anopheles messeae. vi. local selection and 3-dimensional differentiation of the inversion gene complexes for fertility].a field experiment was carried on with the view of compulsory change of the frequency of chromosomal inversion (2r1) in the population of anopheles messeae from the centre of the area. quick return of the population to the original structure was observed. this confirmed that the local differentiation of chromosomal polymorphism is supported by natural selection. the fertility of the females was shown to be connected with the inversion polymorphism. the females with inversion complexes of genes o ...19854085790
[ecology of anopheles messeae fall. in headwaters of the future sayan reservoir (central tuva valley)]. 20093990649
[distribution of the points of breaks in radiation-induced chromosome rearrangements along the polytene chromosomes of anopheles messeae].distribution of 431 rearrangement breaks induced by x-ray treatment in polytene chromosomes of anopheles messeae was studied. no differences were revealed in the distribution pattern of inversion and translocation breaks. the frequency both of inversion and translocation breaks is much greater in the distal parts of autosomes than in the proximal ones. within autosomes, breaks are grouped in a number of subdivisions. the points of breaks are non-randomly distributed. the causes for this non-rand ...19836685671
[blood-sucking activity of ddt-resistant populations of anopheles messeae in the absence and presence of contact with ddt]. 20136646058
[quantitative dose-effect relationships of the mutagenic action of x-rays on the sex cells of the malaria mosquito anopheles messeae].inseminated females of anopheles messeae were irradiated by x-rays (1.2 and 3 kr). estimation of mutagenic effect on the embryonic (dominant lethals) and the postembryonic (chromosomal rearrangements) stages of the development of the f1 offspring is presented. the frequency of dominant lethals varies in accordance with the dosage and the stage of maturity of oocytes. the embryonic lethality depends on induced defects of female sex cells. the frequency of chromosomal rearrangements increases to 3 ...19836685082
[inversion polymorphism of the malarial mosquito anopheles messeae. v. the interaction of different chromosomal inversions in the spatial area].the relations between inversions of chromosome xl, 2r, 3r, 3l from 8 populations of the central part and periphery of the species area were studied. 2-, 3- and 4-factor analysis was carried out. interaction of inversions xl1, 2r1, 3r1 in central populations (riga, moscow, tomsk) was observed. the nonrandom association of definite inversional genotypes was stationary in spatial - temporary. the phylogenetic initial sequences xl0, 2r0, 3r0 display "+" interaction. the 3-factor analysis of populati ...19836682816
[inversion polymorphism of the malarial mosquito anopheles messeae. iv. the stability of the frequency distribution of the inversions by species area].distribution of the frequencies of inversions xl1, 2r1, 3r1, 3l1, from geographically widely separated 8 populations had been studied during a long period of time. the geographical inclines of distribution of inversions xl1, 3r1 (a longitudinal) and 2r1 (a latitudinal) are stable in time. stationarity of inversional polymorphism is not connected with superdominance of heterozygotes. it is supposed that the stable equilibrium of the frequencies of inversions is supported by variable selection.19836682815
[types, frequency and interchromosomal distribution of radiation-induced chromosome rearrangements in the malaria mosquito anopheles messeae].inseminated females of a. messeae caught from their natural population (kolarovo, tomsk) were x-ray-irradiated (1, 2 and 3 kr, 100r/min). salivary gland chromosomes of larvae f1 were used to detect any aberrations induced. the great number of chromosomal aberrations was obtained. the inversions were more frequent (paracentric - 41.0% pericentric - 33.1%), that translocations (25.2%). a single deletion (0.7%) was found. the distribution of 304 breaks induced was studied. frequencies of breaks in ...19827071939
[adaptive association of inversions in a natural population of the malaria mosquito anopheles messeae fall].the dependent combination of chromosomal variants by five paracentric inversions (il1, il2, iir1, iiir1, iiil1) in anopheles messeae population from the west siberia was discovered. it is found that the inversion interactions interactions are the same at both larval and imaginal parts of the population, and they are stable during four years of studying. the inversion of hetero- and homozygotes demonstrates identical properties, that is, phylogenetic secondary sequencies dominate in heterozygotes ...1979467975
[inversion polymorphism in the malarial mosquito, anopheles messeae. iii. the temporal dynamics of the inversion frequencies in the population of the center of a geographic range].the karyotypic structure of the studied population shows the clear temporal differentiation. the frequencies of the homozygotes iir/iir, iiir1/iiir1 and iiil/iiil increase to the middle of the reproductive period. this homozygotes are initial gene arrangements in the phylogenetic sense and their adaptive value is evidently higher than the alternative homozygotes during the population flowering. inter-year correlations of the dynamic frequencies of several gene arrangements appears to be due to d ...1978744477
[study of the karyotype of the malaria mosquito].a study of the karyotype composition of 22 geographycally removed natural populations of the malarial mosquito anopheles messeae fall revealed a chromosomal polymorphism characteristic of the whole species. 13 paracentric inversions were detected, no other gene arrangements being discovered. all the inversions can be divided into two classes: 1) wide spread inversions in hetero- and homozygous forms; 2) endemic ones discovered in individual populations in the form of heterozygotes. according to ...19761027166
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