| novel variant of tickborne encephalitis virus, russia. | we isolated a novel strain of tickborne encephalitis virus (tbev), glubinnoe/2004, from a patient with a fatal case in russia. we sequenced the strain, whose landmark features included 57 amino acid substitutions and 5 modified cleavage sites. phylogenetically, glubinnoe/2004 is a novel variant that belongs to the eastern type of tbev. | 2007 | 18258012 |
| greek goat encephalitis virus strain isolated from ixodes ricinus, greece. | a strain of greek goat encephaltitis virus was isolated from engorged ixodes ricinus ticks that had fed on goats in northern greece. the strain was almost identical to the prototype strain isolated 35 years ago. | 2008 | 18258134 |
| genetic study of the distribution of greek goat encephalitis virus in greece. | tick-borne encephalitis virus (tbev) and louping ill virus (liv) are viruses in the mammalian tick-borne virus group/genus flavivirus, causing central nervous system disease. greek goat encephalitis virus (ggev), which was isolated from the brain of a newborn goat with neurological symptoms, is currently classified in the tbev group. the vector of ggev has not yet been specifically identified but is considered likely to be ixodes ricinus. a total of 3,144 ixodidae ticks collected during 2003-200 ... | 2008 | 18471057 |
| structural and functional analyses of a conserved hydrophobic pocket of flavivirus methyltransferase. | the flavivirus methyltransferase (mtase) sequentially methylates the n7 and 2'-o positions of the viral rna cap (gpppa-rna → m(7)gpppa-rna → m(7)gpppam-rna), using s-adenosyl-l-methionine (adomet) as a methyl donor. we report here that sinefungin (sin), an adomet analog, inhibits several flaviviruses through suppression of viral mtase. the crystal structure of west nile virus mtase in complex with sin inhibitor at 2.0-å resolution revealed a flavivirus-conserved hydrophobic pocket located next t ... | 2010 | 20685660 |
| rapid molecular detection methods for arboviruses of livestock of importance to northern europe. | arthropod-borne viruses (arboviruses) have been responsible for some of the most explosive epidemics of emerging infectious diseases over the past decade. their impact on both human and livestock populations has been dramatic. the early detection either through surveillance or diagnosis of virus will be a critical feature in responding and resolving the emergence of such epidemics in the future. although some of the most important emerging arboviruses are human pathogens, this paper aims to high ... | 2012 | 22219660 |
| revisiting the clinal concept of evolution and dispersal for the tick-borne flaviviruses by using phylogenetic and biogeographic analyses. | tick-borne flaviviruses (tbf) are widely dispersed across africa, europe, asia, oceania, and north america, and some present a significant threat to human health. seminal studies on tick-borne encephalitis viruses (tbev), based on partial envelope gene sequences, predicted a westward clinal pattern of evolution and dispersal across northern eurasia, terminating in the british isles. we tested this hypothesis using all available full-length open reading frame (orf) tbf sequences. phylogenetic ana ... | 2012 | 22674986 |
| louping ill virus: an endemic tick-borne disease of great britain. | in europe and asia, ixodid ticks transmit tick-borne encephalitis virus (tbev), a flavivirus that causes severe encephalitis in humans but appears to show no virulence for livestock and wildlife. in the british isles, where tbev is absent, a closely related tick-borne flavivirus, named louping ill virus (liv), is present. however, unlike tbev, liv causes a febrile illness in sheep, cattle, grouse and some other species, that can progress to fatal encephalitis. the disease is detected predominant ... | 2014 | 24552787 |
| ecuador paraiso escondido virus, a new flavivirus isolated from new world sand flies in ecuador, is the first representative of a novel clade in the genus flavivirus. | a new flavivirus, ecuador paraiso escondido virus (epev), named after the village where it was discovered, was isolated from sand flies (psathyromyia abonnenci, formerly lutzomyia abonnenci) that are unique to the new world. this represents the first sand fly-borne flavivirus identified in the new world. epev exhibited a typical flavivirus genome organization. nevertheless, the maximum pairwise amino acid sequence identity with currently recognized flaviviruses was 52.8%. phylogenetic analysis o ... | 2015 | 26355096 |
| characterization of a novel insect-specific flavivirus from brazil: potential for inhibition of infection of arthropod cells with medically important flaviviruses. | in the past decade, there has been an upsurge in the number of newly described insect-specific flaviviruses isolated pan-globally. we recently described the isolation of a novel flavivirus (tentatively designated 'nhumirim virus'; nhuv) that represents an example of a unique subset of apparently insect-specific viruses that phylogenetically affiliate with dual-host mosquito-borne flaviviruses despite appearing to be limited to replication in mosquito cells. we characterized the in vitro growth p ... | 2014 | 25146007 |
| revisiting recombination signal in the tick-borne encephalitis virus: a simulation approach. | the hypothesis of wide spread reticulate evolution in tick-borne encephalitis virus (tbev) has recently gained momentum with several publications describing past recombination events involving various tbev clades. despite a large body of work, no consensus has yet emerged on tbev evolutionary dynamics. understanding the occurrence and frequency of recombination in tbev bears significant impact on epidemiology, evolution, and vaccination with live vaccines. in this study, we investigated the poss ... | 2016 | 27760182 |
| flavidb: a data mining system for knowledge discovery in flaviviruses with direct applications in immunology and vaccinology. | the flavivirus genus is unusually large, comprising more than 70 species, of which more than half are known human pathogens. it includes a set of clinically relevant infectious agents such as dengue, west nile, yellow fever, and japanese encephalitis viruses. although these pathogens have been studied extensively, safe and efficient vaccines lack for the majority of the flaviviruses. | 0 | 25544857 |
| population seroprevalence study after a west nile virus lineage 2 epidemic, greece, 2010. | during summer 2010, 262 human cases including 35 deaths from west nile virus (wnv) infection were reported from central macedonia, greece. evidence from mosquitoes, birds and blood donors demonstrated that the epidemic was caused by wnv lineage 2, which until recently was considered of low virulence. we conducted a household seroprevalence study to estimate the spread of infection in the population during the epidemic, ascertain the relationship of infection to clinical disease, and identify ris ... | 2013 | 24260390 |
| first dating of a recombination event in mammalian tick-borne flaviviruses. | the mammalian tick-borne flavivirus group (mtbfg) contains viruses associated with important human and animal diseases such as encephalitis and hemorrhagic fever. in contrast to mosquito-borne flaviviruses where recombination events are frequent, the evolutionary dynamic within the mtbfg was believed to be essentially clonal. this assumption was challenged with the recent report of several homologous recombinations within the tick-borne encephalitis virus (tbev). we performed a thorough analysis ... | 2012 | 22384119 |
| structural models for the design of novel antiviral agents against greek goat encephalitis. | the greek goat encephalitis virus (gge) belongs to the flaviviridae family of the genus flavivirus. the gge virus constitutes an important pathogen of livestock that infects the goat's central nervous system. the viral enzymes of gge, helicase and rna-dependent rna polymerase (rdrp), are ideal targets for inhibitor design, since those enzymes are crucial for the virus' survival, proliferation and transmission. in an effort to understand the molecular structure underlying the functions of those v ... | 2014 | 25392762 |