| classification of pseudomonas diminuta leifson and hugh 1954 and pseudomonas vesicularis büsing, döll, and freytag 1953 in brevundimonas gen. nov. as brevundimonas diminuta comb. nov. and brevundimonas vesicularis comb. nov., respectively. | the taxonomic positions of strains previously assigned to pseudomonas diminuta and pseudomonas vesicularis were investigated by a polyphasic approach. the results of dna-rrna hybridization studies indicated that these two species belong to a separate genus in the alpha subclass (rrna superfamily iv) of the proteobacteria, for which the name brevundimonas is proposed. genus delineation and species delineation were determined by comparing the results of numerical analyses of whole-cell protein pat ... | 1994 | 8068543 |
| rapid methods for testing the efficacy of sterilization-grade filter membranes. | the validation of sterilization-grade membranes is integral to ensuring the efficient and safe use of microfiltration systems. here validation refers to the production of sterile filtrate for sterilizing-grade membranes under challenge test conditions. current validation methods require 48 h of culture for results to become available, which creates time delays within the manufacturing process and quality control (qc) backlogs. this work compares four methods for the production of filter challeng ... | 2000 | 10919803 |
| 16s rdna-based identification of bacteria from conjunctival swabs by pcr and dgge fingerprinting. | establishment of a new molecular biology technique for the identification of multiple bacteria from the ocular environment, which can be applied supplementarily to cultivation in cases of severe bacterial infections. | 2001 | 11328723 |
| expression of the iorab genes from brevundimonas diminuta 7 encoding the molybdenum hydroxylase isoquinoline 1-oxidoreductase in pseudomonas putida. | isoquinoline 1-oxidoreductase (ior) from brevundimonas diminuta 7, encoded by iorab, is a molybdenum hydroxylase containing a molybdopterin cytosine dinucleotide molybdenum cofactor (mo-mcd) and two distinct [2fe2s] clusters. the iorab genes were inserted into pjb653, generating pil1. pseudomonas putida kt2440, and p. putida 86 which produces a mo-mcd-containing quinoline 2-oxidoreductase when grown on quinoline, were used as recipients for pil1. upon induction of gene expression, both clones pr ... | 2002 | 12023088 |
| retention of water-borne bacteria by membrane filters. part i: bacterial challenge tests on 0.2 and 0.22 micron rated filters. | the results of bacterial challenge tests conducted on several 0.2 and 0.22 micron rated "sterilizing grade" filter cartridge types with bacteria from a natural water source are presented. eight different 0.2/0.22 micron rated "sterilizing grade" filter types from four different filter manufacturers, claimed to be capable of retaining brevundimonas diminuta at a challenge level of 10(7) cfu/cm2, were tested. the filters tested included nylon 6.6 and polyamide filters from two manufacturers, modif ... | 2001 | 11310322 |
| characterization of oxytetracycline-resistant heterotrophic bacteria originating from hospital and freshwater fishfarm environments in england and ireland. | this ecotaxonomic study compared the antibiotic tolerance among culturable oxytetracyline-resistant (ot(r)) heterotrophic strains isolated from two aquatic environments representing human activities in health care and aquaculture, namely hospital effluents and freshwater fishfarms. using a standardized methodology, samples taken in england and ireland were analyzed to determine the antibiotic tolerance profiles of two groups of culturable ot(r) bacterial isolates at the intergeneric and intragen ... | 2000 | 11249032 |
| determination of the systematic position of the genus asticcacaulis poindexter by a polyphasic analysis. | the genus asticcacaulis, to date, comprises two species of unicellular, stalked bacteria, developing a stalk at a site which is not coincidental with the centre of the pole of the cell. multiplication is similar to that demonstrated by the prosthecate species of the genera caulobacter, brevundimonas and maricaulis. a polyphasic approach, comprising 16s rrna gene sequencing, lipid analysis and nacl tolerance characterizations, was used to clarify the taxonomy of the two asticcacaulis species. fro ... | 2001 | 11211267 |
| alpha- and beta-proteobacteria control the consumption and release of amino acids on lake snow aggregates. | we analyzed the composition of aggregate (lake snow)-associated bacterial communities in lake constance from 1994 until 1996 between a depth of 25 m and the sediment surface at 110 m by fluorescent in situ hybridization with rrna-targeted oligonucleotide probes of various specificity. in addition, we experimentally examined the turnover of dissolved amino acids and carbohydrates together with the microbial colonization of aggregates formed in rolling tanks in the lab. generally, between 40 and m ... | 2001 | 11157226 |
| development and qualification of a novel virus removal filter for cell culture applications. | commercial bioreactors employing mammalian cell cultures to express biological or pharmaceutical products can become contaminated with adventitious viruses. the high expense of such a contamination can be reduced by passing all gases and fluids feeding the bioreactor through virus inactivation or removal steps, which act as viral barriers around the bioreactor. a novel virus barrier filter has been developed for removing viruses from serum-free cell culture media. this filter removes the 20 nm m ... | 2000 | 10835245 |
| hospital-acquired brevundimonas vesicularis septicaemia following open-heart surgery: case report and literature review. | brevundimonas vesicularis (b. vesicularis) is a pseudomonad rarely encountered in human infection. a case of nosocomial septicaemia with this organism following open-heart surgery is presented, with a review of the literature. the isolate demonstrated resistance to ciprofloxacin and aztreonam, which has not yet been reported. treatment with piperacillin/tazobactam resulted in full recovery. a review of the literature reveals that b. vesicularis is a virulent organism involved in serious infectio ... | 2000 | 10716085 |
| capillary electrophoresis-single-strand conformation polymorphism analysis for rapid identification of pseudomonas aeruginosa and other gram-negative nonfermenting bacilli recovered from patients with cystic fibrosis. | we used capillary electrophoresis-single-strand conformation polymorphism (ce-sscp) analysis of pcr-amplified 16s rrna gene fragments for rapid identification of pseudomonas aeruginosa and other gram-negative nonfermenting bacilli isolated from patients with cystic fibrosis (cf). target sequences were amplified by using forward and reverse primers labeled with various fluorescent dyes. the labeled pcr products were denatured by heating and separated by capillary gel electrophoresis with an autom ... | 1999 | 10488211 |
| phylogeny and polyphasic taxonomy of caulobacter species. proposal of maricaulis gen. nov. with maricaulis maris (poindexter) comb. nov. as the type species, and emended description of the genera brevundimonas and caulobacter. | the genus caulobacter is composed of prosthecate bacteria often specialized for oligotrophic environments. the taxonomy of caulobacter has relied primarily upon morphological criteria: a strain that visually appeared to be a member of the caulobacter has generally been called one without challenge. a polyphasic approach, comprising 16s rdna sequencing, profiling restriction fragments of 16s-23s rdna interspacer regions, lipid analysis, immunological profiling and salt tolerance characterizations ... | 1999 | 10425763 |
| the phylogenetic relationships of caulobacter, asticcacaulis and brevundimonas species and their taxonomic implications. | the phylogenetic relationships among the species of caulobacter, asticcacaulis and brevundimonas were studied by comparison of their 16s rdna sequences. the analysis of almost complete sequences confirmed the early evolutionary divergence of the freshwater and marine species of caulobacter reported previously [stahl, d. a., key, r., flesher, b. & smit, j. (1992). j bacteriol 174, 2193-2198]. the freshwater species formed two distinct clusters. one cluster contained the species caulobacter bacter ... | 1999 | 10319468 |
| comparison of the fuel oil biodegradation potential of hydrocarbon-assimilating microorganisms isolated from a temperate agricultural soil. | strains of hydrocarbon-degrading microorganisms (bacteria and fungi) were isolated from an agricultural soil in france. in a field, a portion was treated with oily cuttings resulting from the drilling of an onshore well. the cuttings which were spread at the rate of 600 g hc m-2 contained 10% of fuel oil hydrocarbons (hc). another part of the field was left untreated. three months after hc spreading, hc adapted bacteria and fungi were isolated at different soil depths in the two plots and identi ... | 1999 | 10231986 |
| preparation and evaluation of bacterial stocks for filter validation. | bacterial cell stock paste of brevundimonas diminuta (american type culture collection # 19146) used for filter validation was evaluated by standard microbiological procedures to determine culture purity, monodispersion, cell size, and strain identity. tests performed included direct microscopic count, standard plate count, gram stain, streak plate, sizing by microfiltration, scanning electron microscopy, and biochemical identification. results indicated the bacterial stocks were of consistent q ... | 1997 | 9277123 |
| structural studies of malate dehydrogenases (mdhs): mdhs in brevundimonas species are the first reported mdhs in proteobacteria which resemble lactate dehydrogenases in primary structure. | the n-terminal sequences of malate dehydrogenases from 10 bacterial strains, representing seven genera of proteobacteria, were determined. of these, the enzyme sequences of species classified in the genus brevundimonas clearly resembled those malate dehydrogenases with greatest similarity to lactate dehydrogenases. additional evidence from subunit molecular weights, peptide mapping, and enzyme mobilities suggested that malate dehydrogenases from species of the genus brevundimonas were structural ... | 1997 | 9190829 |
| reassessment of the phylogenetic position of caulobacter subvibrioides. | determination of the 16s rrna gene sequence of caulobacter subvibrioides atcc 15264t (t = type strain) confirmed that this species is a member of the alpha subclass of the proteobacteria and showed that it is phylogenetically most closely related to the caulobacter group comprising the species caulobacter bacteroides, caulobacter crescentus, and brevundimonas (pseudomonas) diminuta, for which 16s rrna sequences of the type strains are currently available. the closest known relative of strain atc ... | 1997 | 8995825 |
| metabolic characterisation of a novel vanillylmandelate-degrading bacterium. | a newly isolated gram-negative bacterium, possibly brevundimonas diminuta, utilised d,l-vanillylmandelate (d,l-vma) as a sole carbon and energy source. the organism converted d,l-vma to vanillylglyoxylate using a soluble nad-dependent dehydrogenase specific for d-vma and a dye-linked, membrane-associated l-vma dehydrogenase. vanillylglyoxylate was further metabolised by decarboxylation, dehydrogenation and demethylation to protocatechuate. a 4,5-dioxygenase cleaved protocatechuate to 2-hydroxy-4 ... | 1996 | 8824148 |
| comparative ribosomal protein sequence analyses of a phylogenetically defined genus, pseudomonas, and its relatives. | i analyzed various families of ribosomal proteins obtained from selected species belonging to the genus pseudomonas sensu stricto and allied organisms which were previously classified in the genus pseudomonas. partial amino acid sequencing of l30 preparations revealed that the strains which i examined could be divided into three clusters. the first cluster, which was assigned to the genus pseudomonas sensu stricto, included pseudomonas aeruginosa, pseudomonas putida, pseudomonas mendocina, and p ... | 1995 | 7727274 |
| performance of a novel viresolve nfr virus filter. | mammalian cell-expressed therapeutic proteins are particularly vulnerable to contamination by endogenous retrovirus-like particles (rvlps). the viresolve nfr filter was designed to meet the critical requirement of manufacturing a safe and virus-free therapeutic by retaining rvlps by a minimum of six log reduction value (lrv). the nfr designation refers to retrovirus removal in a normal flow format. to qualify the product, we tested two model viruses: the 78 nm diameter phi6 bacteriophage and the ... | 2002 | 12153313 |
| identification of an opd (organophosphate degradation) gene in an agrobacterium isolate. | we isolated a bacterial strain, agrobacterium radiobacter p230, which can hydrolyze a wide range of organophosphate (op) insecticides. a gene encoding a protein involved in op hydrolysis was cloned from a. radiobacter p230 and sequenced. this gene (called opda) had sequence similarity to opd, a gene previously shown to encode an op-hydrolyzing enzyme in flavobacterium sp. strain atcc 27551 and brevundimonas diminuta mg. insertional mutation of the opda gene produced a strain lacking the ability ... | 2002 | 12089017 |
| prevalent bacterial species and novel phylotypes in advanced noma lesions. | the purpose of this study was to determine the bacterial diversity in advanced noma lesions using culture-independent molecular methods. 16s ribosomal dna bacterial genes from dna isolated from advanced noma lesions of four nigerian children were pcr amplified with universally conserved primers and spirochetal selective primers and cloned into escherichia coli. partial 16s rrna sequences of approximately 500 bases from 212 cloned inserts were used initially to determine species identity or close ... | 2002 | 12037085 |
| changes in the cell size of brevundimonas diminuta using different growth agitation rates. | brevundimonas diminuta (atcc 19146) is a standard organism for validation of sterilizing-grade membrane filters. cell size is critical for the determination of retention characteristics of 0.2 micron rated membrane filters. in this study, cell size changes of b. diminuta cultured under different physiologic states and variable agitations at 50, 100 and 200 rpm were measured by a particle size analyzer and scanning electron microscope (sem). the smallest cells were obtained at initial stationary ... | 2002 | 11977409 |
| classification of a brevundimonas strain detectable after pcr with a helicobacter-specific primer pair. | in a study for the isolation of new helicobacter strains, biopsy samples were taken from the gastric mucosa of dogs and subjected to pcr amplification using a helicobacter-specific primer pair (h276f and h676r, directed to the 16s rdna) to identify members of this genus in the specimens. from one helicobacter positive sample, a bacterial strain was isolated which displayed a characteristic band after pcr amplification with the helicobacter-specific primer pair. the isolate designated h2/98-fundu ... | 2001 | 11822672 |
| [auxotrophy and utilization of oxidized and reduced mineral sulfur forms by brevundimonas diminuta strains]. | connection between auxotrophy of brevundimonas diminuta strains for sulphur-containing amino acids and presence of oxidized or reduced sulphur forms in the medium has been shown. when grown in synthetic medium supplied with sulphate or sulphite b. diminuta strains required cystine, biotin, pantothenate and vitamin b12 for growth and utilized very limited spectrum--from 8 to 12--carbon sources. in the presence of 5 g/l of thiosulphate in the medium instead of mentioned sulphur-containing salts st ... | 2001 | 11785417 |
| method for qualifying microbial removal performance of 0.1 micron rated filters. part iii: bacterial challenge tests on 0.2/0.22 and 0.1 micron rated filter cartridges with hydrogenophaga (formerly pseudomonas) pseudoflava. | we have previously reported on the preliminary characterization of hydrogenophaga (formerly pseudomonas) pseudoflava for potential use as a standard challenge organism to qualify 0.1 microm rated filters. this article reports on the retention efficiencies of a large panel of 0.2/0.22 microm and 0.1 microm rated filter cartridges for h. pseudoflava (atcc 700892) versus the retention capabilities of the same filters for brevundimonas diminuta (atcc 19146). a total of thirty-two 0.2/0.22 microm rat ... | 2001 | 11766824 |
| method for qualifying microbial removal performance of 0.1 micron rated filters. part ii: preliminary characterization of hydrogenophaga (formerly pseudomonas) pseudoflava for use as a standard challenge organism to qualify 0.1 micron rated filters. | in this article, we report on the preliminary characterization of hydrogenophaga (formerly pseudomonas) pseudoflava for potential use as a standard challenge organism to qualify 0.1 microm rated filters. filter-cloned h. pseudoflava (atcc 700892) was easily cultured in a low nutrient broth (r2a broth) under standard laboratory conditions, reaching high titers of 10(8)-10(9) cfu/ml within 48-65 hours of incubation at 25+/-5 degrees c. under these conditions, h. pseudoflava is a rod-shaped bacteri ... | 2001 | 11766823 |
| phylogenies of atpd and reca support the small subunit rrna-based classification of rhizobia. | the current classification of the rhizobia (root-nodule symbionts) assigns them to six genera. it is strongly influenced by the small subunit (16s, ssu) rrna molecular phylogeny, but such single-gene phylogenies may not reflect the evolution of the genome as a whole. to test this, parts of the atpd and reca genes have been sequenced for 25 type strains within the alpha-proteobacteria, representing species in rhizobium, sinorhizobium, mesorhizobium, bradyrhizobium, azorhizobium, agrobacterium, ph ... | 2001 | 11760945 |
| bacteria isolated from the different developmental stages and larval organs of the obligate parasitic fly, wohlfahrtia magnifica (diptera: sarcophagidae). | wohlfahrtia magnifica (diptera: sarcophagidae) is the major myiasis-causing fly species in the whole of eurasia for most important domestic animals. the aim of the present work was to obtain data on the culturable bacteria isolated under aerobic conditions from this fly: bacteria were isolated from all developmental stages (larvae, pupa, and imago) of wohlfahrtia magnifica, and the third-stage larval organs were also sampled. to determine the possible antagonistic effects between the dominant ba ... | 2006 | 16382282 |
| identity and potential functions of heterotrophic bacterial isolates from a continuous-upflow fixed-bed reactor for denitrification of drinking water with bacterial polyester as source of carbon and electron donor. | a collection of 186 heterotrophic bacteria, isolated directly from a continuous-upflow fixed-bed reactor for the denitrification of drinking water, in which poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (phbv) granules acted as biofilm carrier, carbon source and electron donor, was studied with regard to taxonomic affiliation and degradation and denitrification characteristics. two granule samples were taken from a fully operating reactor for enumeration and isolation of heterotrophic bacteria. o ... | 2001 | 11518336 |
| microbial retention characteristics of sterilizing-grade membrane filters with alginate substituted for oil-based products. | for oil-based products, fda recommends substitution of the oil with a compound which has similar viscosity and physical characteristics. in this study, a substitute for oil-based products was screened by measuring the viscosity and filterability, and examined for the presence of cell clumps in the various test fluids using an optical microscopy. the viscosity of the test fluids measured in the range of about 60-75 cp. brevundimonas diminuta (formerly pseudomonas diminuta), a standard challenge t ... | 2002 | 12404720 |
| structural elucidation of the eps of slime producing brevundimonas vesicularis sp. isolated from a paper machine. | the slime forming bacteria brevundimonas vesicularis sp. was isolated from a paper mill and its eps was produced on laboratory scale. after production, the exopolysaccharide (eps) was purified and analysed for its purity and homogeneity, hpsec revealed one distinct population with a molecular mass of more than 2,000 kda. the protein content was around 9 w/w%. the sample was analysed to determine its chemical structure. the eps was found to consist of rhamnose, glucose, galacturonic acid and gluc ... | 2002 | 12431884 |
| diversity of tetracycline resistance genes in bacteria from chilean salmon farms. | twenty-five distinct tetracycline-resistant gram-negative bacteria recovered from four chilean fish farms with no history of recent antibiotic use were examined for the presence of tetracycline resistance (tet) genes. sixty percent of the isolates carried 1 of the 22 known tet genes examined. the distribution was as follows. the tet(a) gene was found in six isolates. the tet(b) gene was found in two isolates, including the first description in the genus brevundimonas: two isolates carried the te ... | 2003 | 12604516 |
| antimicrobial activity of aqueous and methanol extracts of juniperus oxycedrus l. | aqueous and methanol extracts of the leaves of juniperus oxycedrus were investigated for their in vitro antimicrobial properties. the plant was collected from pelitli village of gebze, kocaeli, in the marmara region of turkey. juniperus oxycedrus is widely used as traditional folk medicine in turkey for treatment of different infectious diseases. the antimicrobial activity of the extracts against 143 laboratory strains belonging to 56 bacterial species, and 31 isolates of 5 fungi species were ev ... | 2003 | 12639746 |
| characterisation of bacterial cultures enriched on the chlorophenoxyalkanoic acid herbicides 4-(2,4-dichlorophenoxy) butyric acid and 4-(4-chloro-2-methylphenoxy) butyric acid. | the aim of this study was to enrich and characterise bacterial consortia from soils around a herbicide production plant through their capability to degrade the herbicides 4-(2,4-dichlorophenoxy) butyric acid (2,4-db) and 4-(4-chloro-2-methylphenoxy) butyric acid (mcpb). partial 16s rrna gene sequencing revealed members of the genera stenotrophomonas, brevundimonas, pseudomonas, and ochrobactrum in the 2,4-db- and mcpb-degrading communities. the degradation of 2,4-db and mcpb was facilitated by t ... | 2003 | 14513383 |
| biodegradation of dna and nucleotides to nucleosides and free bases. | thirty-two different microorganisms were examined in order to check their ability to degrade an exogenous dna. bacteria from species: stenotrophomonas maltophilia, brevundimonas diminuta, bacillus subtilis, mycobacterium butyricum and fungus fusarium moniliforme were capable to degrade dna to nucleic bases or their derivatives. degradation of dna by s. maltophilia resulted in formation of free bases, such as hypoxanthine, thymine, uracil and xanthine. the optimum concentration of dna seemed to b ... | 2004 | 14751311 |
| brevundimonas bacteremia: two case reports and literature review. | brevundimonas spp. are infrequently isolated from clinical samples, including blood. the vast majority of reported cases have underlying diseases. whether these microorganisms represent true pathogens of human disease in all populations remains to be explored. we present 2 cases of bloodstream infection caused by brevundimonas sp., with one of these individuals immunocompetent, and discuss the possible role of these organisms in different patient populations. | 2004 | 15000562 |
| bacterial diversity of the inner mongolian baer soda lake as revealed by 16s rrna gene sequence analyses. | bacterial diversity associated with baer soda lake in inner mongolia of china was investigated using a culture-independent method. bacterial 16s rrna gene libraries were generated using bacterial oligonucleotide primers, and 16s rrna gene sequences of 58 clones were analyzed phylogenetically. the library was dominated by 16s rdnas of gram-negative bacteria (24% alpha-proteobacteria, 31% beta-proteobacteria, 33% gamma-proteobacteria, and 2% delta-proteobacteria), with a lower percentage of clones ... | 2004 | 15064989 |
| sphingomonas yabuuchiae sp. nov. and brevundimonas nasdae sp. nov., isolated from the russian space laboratory mir. | on the basis of phenotypic and genotypic characteristics and 16s rrna gene sequence analysis, novel species belonging to the genera sphingomonas and brevundimonas were identified from samples taken from the russian space laboratory mir. strain a1-18(t) was isolated from the air. 16s rdna sequence analysis showed that strain a1-18(t) formed a coherent cluster with sphingomonas sanguinis, sphingomonas parapaucimobilis, sphingomonas paucimobilis and sphingomonas roseiflava with sequence similarity ... | 2004 | 15143030 |
| ecological significance of microdiversity: identical 16s rrna gene sequences can be found in bacteria with highly divergent genomes and ecophysiologies. | a combination of cultivation-based methods with a molecular biological approach was used to investigate whether planktonic bacteria with identical 16s rrna gene sequences can represent distinct eco- and genotypes. a set of 11 strains of brevundimonas alba were isolated from a bacterial freshwater community by conventional plating or by using a liquid most-probable-number (mpn) dilution series. these strains had identical 16s rrna gene sequences and represented the dominant phylotype in the plate ... | 2004 | 15294821 |
| bacterial diversity in aortic aneurysms determined by 16s ribosomal rna gene analysis. | aortic aneurysms are common vascular conditions that cause considerable morbidity and mortality. understanding of the mechanisms involved in the pathogenesis of the condition remains limited. recently, infection has been suggested as possible contributor in the development of the disease. the aim of the present study was to examine aortic aneurysms for the presence of bacterial dna using polymerase chain reaction (pcr) targeting the 16s ribosomal rna (rrna) gene, followed by cloning and sequenci ... | 2006 | 17098542 |
| bacterial diversity of a soil sample from schirmacher oasis, antarctica. | the bacterial diversity of a soil sample collected in the vicinity of lake zub, schirmacher oasis, antarctica, was determined both by establishing pure colonies of culturable bacteria and by cloning the total 16s rdna of the soil and establishing the phylogeny of the clones. analysis of the 16s rrna gene clones indicated that the bacteria belonged to the classes alpha-proteobacteria, beta-proteobacteria, gamma-proteobacteria, gemmatimonas, bacteriodetes, actinobacteria, chloroflexi and chlamydia ... | 2004 | 15559969 |
| brevundimonas mediterranea sp. nov., a non-stalked species from the mediterranean sea. | six strains of gram-negative, rod-shaped, non-spore-forming bacteria were isolated from the mediterranean sea. 16s rrna gene sequence analysis indicated that the strains were affiliated within the alphaproteobacterial genus brevundimonas, with brevundimonas intermedia (99.4 %) and brevundimonas vesicularis (99.2 %) as their closest relatives. this affiliation was supported by chemotaxonomic data (major polar lipids: phosphatidyl diacylglycerol, sulfoquinovosyl diacylglycerol and phosphatidyl glu ... | 2005 | 15653922 |
| in vitro suppression of fungi caused by combinations of apparently non-antagonistic soil bacteria. | we hypothesized that apparently non-antagonistic soil bacteria may contribute to suppression of fungi during competitive interactions with other bacteria. four soil bacteria (brevundimonas sp., luteibacter sp., pedobacter sp. and pseudomonas sp.) that exhibited little or no visible antifungal activity on different agar media were prescribed. single and mixed strains of these species were tested for antagonism on a nutrient-poor agar medium against the plant pathogenic fungi fusarium culmorum and ... | 2007 | 17233750 |
| 17beta-estradiol-degrading bacteria isolated from activated sludge. | fourteen phylogenetically diverse 17beta-estradiol-degrading bacteria (strains kc1-14) were isolated from activated sludge of a wastewater treatment plant. these isolates widely distributed among eight different genera--aminobacter (strains kc6 and kc7), brevundimonas (strain kc12), escherichia (strain kc13), flavobacterium (strain kc1), microbacterium (strain kc5), nocardioides (strain kc3), rhodococcus (strain kc4), and sphingomonas (strains kc8-kc11 and kc14)--of three phyla: proteobacteria, ... | 2007 | 17310711 |
| nosocomial brevundimonas vesicularis meningitis. | brevundimonas vesicularis infrequently causes human infections. we describe a case of meningitis due to brevundimonas vesicularis, resistant to piperacillin, gentamicin and amikacin as well as to cephalosporins, aztreonam, imipenem and meropenem. the meningitis was acquired in hospital by a patient operated for astrocytoma and represents a classical hospital infection. | 2006 | 17380092 |
| brevundimonas diminuta infections and its resistance to fluoroquinolones. | to report infections caused by brevundimonas diminuta and antibiotic studies of this gram-negative bacterium. | 2005 | 15883180 |
| identification of bacteria on the surface of clinically infected and non-infected prosthetic hip joints removed during revision arthroplasties by 16s rrna gene sequencing and by microbiological culture. | it has been postulated that bacteria attached to the surface of prosthetic hip joints can cause localised inflammation, resulting in failure of the replacement joint. however, diagnosis of infection is difficult with traditional microbiological culture methods, and evidence exists that highly fastidious or non-cultivable organisms have a role in implant infections. the purpose of this study was to use culture and culture-independent methods to detect the bacteria present on the surface of prosth ... | 2007 | 17501992 |
| papaya shoot tip associated endophytic bacteria isolated from in vitro cultures and host-endophyte interaction in vitro and in vivo. | fourteen distinct bacterial clones were isolated from surface-sterilized shoot tips (approximately 1 cm) of papaya (carica papaya l. 'surya') planted on murashige and skoog (ms)-based papaya culture medium (23/50 nos.) during the 2-4 week period following in vitro culturing. these isolates were ascribed to six gram-negative genera, namely pantoea (p. ananatis), enterobacter (e. cloacae), brevundimonas (b. aurantiaca), sphingomonas, methylobacterium (m. rhodesianum), and agrobacterium (a. tumefac ... | 2007 | 17538647 |
| l: -stereoselective amino acid amidase with broad substrate specificity from brevundimonas diminuta: characterization of a new member of the leucine aminopeptidase family. | brevundimonas diminuta tpu 5720 produces an amidase acting l-stereoselectively on phenylalaninamide. the enzyme (laaa(bd)) was purified to electrophoretic homogeneity by ammonium sulfate fractionation and four steps of column chromatography. the final preparation gave a single band on sds-page with a molecular weight of approximately 53,000. the native molecular weight of the enzyme was about 288,000 based on gel filtration chromatography, suggesting that the enzyme is active as a homohexamer. i ... | 2006 | 16001251 |
| characterization of beta-carotene ketolases, crtw, from marine bacteria by complementation analysis in escherichia coli. | a complementation analysis was performed in escherichia coli to evaluate the efficiency of beta-carotene ketolases (crtw) from the marine bacteria brevundimonas sp. sd212, paracoccus sp. pc1 (alcaligenes pc-1), and paracoccus sp. n81106 (agrobacterium aurantiacum), for astaxanthin production. each crtw gene was expressed in escherichia coli synthesizing zeaxanthin due to the presence of plasmid paccar25deltacrtx. carotenoids that accumulated in the resulting e. coli transformants were examined b ... | 2005 | 16007373 |
| elucidation of a carotenoid biosynthesis gene cluster encoding a novel enzyme, 2,2'-beta-hydroxylase, from brevundimonas sp. strain sd212 and combinatorial biosynthesis of new or rare xanthophylls. | a carotenoid biosynthesis gene cluster mediating the production of 2-hydroxyastaxanthin was isolated from the marine bacterium brevundimonas sp. strain sd212 by using a common crti sequence as the probe dna. a sequence analysis revealed this cluster to contain 12 open reading frames (orfs), including the 7 known genes, crtw, crty, crti, crtb, crte, idi, and crtz. the individual orfs were functionally analyzed by complementation studies using escherichia coli that accumulated various carotenoid p ... | 2005 | 16085816 |
| bacteriophages of freshwater brevundimonas vesicularis isolates. | nine strains of brevundimonas vesicularis were isolated from surface water of three ponds in bielefeld, germany. with those strains as indicators seven bacteriophages with different host ranges were isolated. molecular characterization showed that all phages contained linear double-stranded dna with a similar genome size of about 37 kb. restriction analysis and hybridization of phage dnas revealed that three of these phages are closely related to each other. these phages had morphologies typical ... | 2006 | 16183261 |
| initial stages of bacterial fouling during dead-end microfiltration. | constant pressure experiments were performed using track-etched polycarbonate membranes and rod-shaped bacteria (viz., brevundimonas diminuta and serratia marcescens) to study flux decline and backwashing during the early stages of microfiltration. the intermediate blocking law originally derived for spherical particles was modified to account for the approximate cylindrical shape of the selected bacteria. a deposition factor was introduced to empirically account for the morphology of bacterial ... | 2005 | 16190201 |
| denaturing gradient gel electrophoresis analyses of microbial community from field-scale composter. | the diversity of microbial community during the decomposition of waste in a field-scale composter (hazaka system) was investigated by denaturing gradient gel electrophoresis (dgge). the composter operates at a high temperature through a self-heating system, creating a thermophilic (60-76 degrees c) stage during the initial phase and a mesophilic (45 degrees c) stage towards the later phase of the composting period. the ph of the system (ph 7.75-8.10) did not vary significantly during the process ... | 2001 | 16232968 |
| bacterial communities in petroleum oil in stockpiles. | bacterial communities in crude-oil samples from japanese oil stockpiles were investigated by 16s rrna gene cloning, followed by denaturing gradient gel electrophoresis (dgge) analysis. 16s rrna genes were successfully amplified by pcr after isooctane treatment from three kinds of crude-oil sample collected at four oil stockpiles in japan. dgge profiles showed that bacteria related to ochrobactrum anthropi, burkholderia cepacia, stenotrophomonas maltophilia, propionibacterium acnes, and brevundim ... | 2005 | 16233771 |
| convenient treatment of acetonitrile-containing wastes using the tandem combination of nitrile hydratase and amidase-producing microorganisms. | this study aimed to construct an acetonitrile-containing waste treatment process by using nitrile-degrading microorganisms. to degrade high concentrations of acetonitrile, the microorganisms were newly acquired from soil and water samples. although no nitrilase-producing microorganisms were found to be capable of degrading high concentrations of acetonitrile, the resting cells of rhodococcus pyridinivorans s85-2 containing nitrile hydratase could degrade acetonitrile at concentrations as high as ... | 2006 | 16402166 |
| effect of disinfectants on microbial ecology in model distribution systems. | this research was conducted to assess the impact of various disinfectants on bacterial water quality within model distribution systems (i.e. annular reactors). after colonization with non-disinfected water, annular reactors were treated with relatively low doses of chlorine (0.4 mg/l), chlorine dioxide (0.15 mg/l), or chloramines (0.9 mg/l). under the tested conditions, bacterial inactivation varied as a function of disinfectant type (ranking by efficiency per mg of oxidant: clo2 > cl2 > clnh2) ... | 2005 | 16459842 |
| on the purification and preliminary crystallographic analysis of isoquinoline 1-oxidoreductase from brevundimonas diminuta 7. | isoquinoline 1-oxidoreductase (ior) from brevundimonas diminuta is a mononuclear molybdoenzyme of the xanthine-dehydrogenase family of proteins and catalyzes the conversion of isoquinoline to isoquinoline-1-one. its primary sequence and behaviour, specifically in its substrate specificity and lipophilicity, differ from other members of the family. a crystal structure of the enzyme is expected to provide an explanation for these differences. this paper describes the crystallization and preliminar ... | 2005 | 16508115 |
| brevundimonas kwangchunensis sp. nov., isolated from an alkaline soil in korea. | two gram-negative, rod-shaped bacterial strains, ksl-102t and ksl-110, were isolated from an alkaline soil in korea, and their taxonomic positions were investigated by use of a polyphasic study. the two strains grew optimally at ph 7.0-8.0 and 30 degrees c without nacl. they contained q-10 as the predominant ubiquinone. the major fatty acids were c(18:1)omega7c and c(16:0) on trypticase soy agar, but 11-methyl c(18:1)omega7c was also a major component when the two strains were cultivated on lmg ... | 2006 | 16514037 |
| study of the bacterial load in a gelatine production process focussed on bacillus and related endosporeforming genera. | gelatine is an animal protein with many industrial applications. previous studies pointed out that endosporeforming bacteria, belonging to the genus bacillus or related genera, might contaminate and survive the production process of gelatine, leading to products of low quality and safety. the aim of this study is to determine the bacterial diversity of contaminants isolated from a gelatine production chain with emphasis on aerobic endosporeforming bacteria. contaminants were isolated from sample ... | 2002 | 12583722 |
| characterization of bacterial beta-carotene 3,3'-hydroxylases, crtz, and p450 in astaxanthin biosynthetic pathway and adonirubin production by gene combination in escherichia coli. | beta-carotene hydroxylase (crtz) is one of rate-limiting enzymes for astaxanthin production. a complementation analysis was conducted using escherichia coli transformants to compare the catalytic efficiency of bacterial crtz from brevundimonas sp. sd212, paracoccus sp. pc1 (formerly known as alcaligenes sp. pc-1), paracoccus sp. n81106 (agrobacterium aurantiacum), pantoea ananatis (erwinia uredovora 20d3), marine bacterium p99-3, and p450 monooxygenase (cyp175a1) from thermus thermophilus hb27. ... | 2006 | 16614859 |
| a carotenoid synthesis gene cluster from a non-marine brevundimonas that synthesizes hydroxylated astaxanthin. | a brevundimonas vesicularis strain dc263 isolated from surface soil was shown to produce hydroxylated astaxanthin. a carotenoid synthesis gene cluster containing ten genes was cloned from strain dc263, among which eight genes were involved in carotenoid synthesis. in addition to the crtw gene encoding the 4,4'-beta-ionone ring ketolase and the crtz gene encoding the 3,3'-beta-ionone ring hydroxylase that were responsible for astaxanthin synthesis, the cluster also contained a novel gene crtg ide ... | 2006 | 16781830 |
| evaluation of pyrrolidonyl arylamidase for the identification of nonfermenting gram-negative rods. | to evaluate the activity of pyrrolidonyl arylamidase (pyr) for the differentiation and identification of nonfermenting gram negative rods (nfgnr), 293 isolates were tested. a 24 h culture of each test organism was prepared. from this a 108-109 cfu/ml suspension was added to 0.25 ml of sterile physiologic solution. a pyr disk was then added and the test was incubated for 30 minutes at 35-37 degrees c, at environmental atmosphere. reading was done by adding 1 drop of cinnamaldehyde reagent. strain ... | 2007 | 16822636 |
| capd peritonitis due to brevundimonas vesicularis. | | 2006 | 16881352 |
| meganema perideroedes gen. nov., sp. nov., a filamentous alphaproteobacterium from activated sludge. | an industrial wastewater treatment plant at grindsted, denmark, has suffered from bulking problems for several years caused by filamentous bacteria. five strains were isolated from the sludge by micromanipulation. phylogenetic analysis of the 16s rrna gene sequences showed that the strains formed a monophyletic cluster in the alphaproteobacteria, and they were phenotypically different from their closest relatives and from all hitherto known filamentous bacteria described (closest relative brevun ... | 2006 | 16902022 |
| brevundimonas vesicularis septic arthritis in an immunocompetent child. | septic arthritis is a rapidly destructive form of joint disease. the most common causative agents in children are staphylococcus aureus and kingella kingae, followed by group a streptococcus and streptococcus pneumoniae, and in neonates, enterobacteracea and group b streptococcus. in this paper, we describe a previously healthy toddler with septic arthritis of the shoulder joint caused by brevundimonas vesicularis. prompt treatment with cefuroxime resulted in a full recovery. this is the first r ... | 2007 | 16941131 |
| utilization of hexamethylenetetramine (urotropine) by bacteria and yeasts. | a slow growing bacterial population able to utilize hexamethylelenetetramine (urotropine) as sole source of carbon, nitrogen and energy was isolated from soil. from this crude enrichment culture two bacteria were isolated and identified as brevundimonas diminuta and a phyllobacterium sp. by sequencing of 16s ribosomal dna. these bacteria also grew on urotropine but at a lower rate than the enrichment culture. addition of glucose to the latter resulted in growth of some yeasts that overgrew the b ... | 2007 | 17043911 |
| brevundimonas terrae sp. nov., isolated from an alkaline soil in korea. | a gram-negative, rod-shaped, brevundimonas-like bacterial strain, ksl-145(t), was isolated from an alkaline soil in korea and subjected to a polyphasic taxonomic investigation. strain ksl-145(t) grew optimally at ph 7.5-8.0 and 30 degrees c without nacl. it was characterized chemotaxonomically as containing q-10 as the predominant ubiquinone and c(18 : 1)omega7c and c(16 : 0) as the major fatty acids. the dna g+c content was 61.8 mol%. a phylogenetic analysis based on 16s rrna gene sequences sho ... | 2006 | 17158998 |
| case report: infective endocarditis caused by brevundimonas vesicularis. | there are few reports in the literature of invasive infection caused by brevundimonas vesicularis in patients without immunosuppression or other predisposing factors. the choice of antimicrobial therapy for bacteremia caused by the pathogen requires more case experience to be determined. | 2006 | 17194310 |
| polysaccharide-producing bacteria isolated from paper machine slime deposits. | development of novel enzymatic methods for slime deposit control in paper mills requires knowledge of polysaccharide-producing organisms and the polysaccharide structures present in deposits. in this work, 27 polysaccharide-producing bacteria were isolated from slime samples collected from different parts of a paper machine. most of the isolates produced polysaccharides in liquid culture and nine of them were selected for production of polysaccharides for characterisation. the selected isolates ... | 2005 | 15750806 |
| evidence of pathogenic microbes in the international space station drinking water: reason for concern? | molecular analyses were carried out on four preflight and six postflight international space station (iss)-associated potable water samples at various stages of purification, storage, and transport, to ascertain their associated microbial diversities and overall microbial burdens. following dna extraction, pcr amplification, and molecular cloning procedures, rdna sequences closely related to pathogenic species of acidovorax, afipia, brevundimonas, propionibacterium, serratia, and others were rec ... | 2004 | 15880908 |
| adhesion and viability of waterborne pathogens on p-dadmac coatings. | the attachment of waterborne pathogens onto surfaces can be increased by coating the surfaces with positive charge-enhancing polymers. in this paper, the increased efficacy of polydiallyldimethylammonium chloride (p-dadmac) coatings on glass was evaluated in a parallel plate flow chamber with the use of waterborne pathogens (raoultella terrigena, escherichia coli, and brevundimonas diminuta). p-dadmac coatings strongly compensated the highly negative charges on the glass surface and even yielded ... | 2008 | 17570712 |
| characterization of aerobic bacterial and fungal microbiota on surfaces of historic scottish monuments. | twenty samples were taken from the inner or outer surfaces of stone monuments of six historic scottish buildings and ruins. biofilms developing on mineral substrates were analysed by in situ scanning electron microscopy and cultivation. various methods were used to characterize the isolates including automated ribotyping, rapd and sequencing of the 16s rrna gene for bacteria, and stereomicroscopy and sequencing of the internal transcribed spacers (its) for fungi. most samples contained microbes ... | 2007 | 17583461 |
| brevundimonas aveniformis sp. nov., a stalked species isolated from activated sludge. | a gram-negative, rod-like, stalk-producing bacterium, designated strain emb102(t), was isolated from activated sludge that performed enhanced biological phosphorus removal in a sequencing batch reactor. cells without stalks were motile with single polar flagella, but cells that did produce stalks were non-motile and lacked polar flagella. growth of strain emb102(t) was observed at temperatures between 15 and 35 degrees c (optimum, 30 degrees c) and between ph 6.0 and 9.0 (optimum, ph 7.5-8.5). t ... | 2007 | 17625194 |
| biosynthesis of poly-beta-hydroxyalkanoate by brevundimonas vesicularis lmg p-23615 and sphingopyxis macrogoltabida lmg 17324 using acid-hydrolyzed sawdust as carbon source. | poly-beta-hydroxyalkanoate (pha) is a biodegradable polymer accumulated in intracellular granules by different bacterial species. its physical and chemical properties are similar to those of petroleum-derived plastics. material generated by the acid hydrolysis of wood was evaluated for use in the bacterial synthesis of pha. acid-hydrolyzed sawdust was prepared and adjusted to ph 7. mineral salts with carbon:nitrogen (c:n) proportions of 100:1, 100:3.5, 100:10, 100:30, or 100:50 and trace element ... | 2007 | 17630126 |
| bacterial contamination of water in dental unit reservoirs. | the aim of this study was bacteriological assessment of water in dental unit reservoirs--concentration and composition of the aerobe and facultative anaerobe bacterial microflora. reservoir water samples were taken from 25 units. bacterial flora were determined with the plate culture method. bacteria were identified with biochemical microtests: api 20e, api 20ne (biomérieux, france) and gp2 microplatetm (biolog, usa). the concentration of total bacteria isolated from one site was 201,039 cfu/ml, ... | 2007 | 17655191 |
| bismuth dimercaptopropanol (bisbal) inhibits the expression of extracellular polysaccharides and proteins by brevundimonas diminuta: implications for membrane microfiltration. | a 2:1 molar ratio preparation of bismuth with a lipophilic dithiol (3-dimercapto-1-propanol, bal) significantly reduced extracellular polymeric substances (eps) expression by brevundimonas diminuta in suspended cultures at levels just below the minimum inhibitory concentration (mic). total polysaccharides and proteins secreted by b. diminuta decreased by approximately 95% over a 5-day period when exposed to the bismuth-bal chelate (bisbal) at near mic (12 microm). fourier-transform infrared spec ... | 2008 | 17705249 |
| brevundimonas lenta sp. nov., isolated from soil. | a gram-negative, rod-shaped, brevundimonas-like bacterial strain, ds-18(t), was isolated from soil in dokdo, korea, and its exact taxonomic position was investigated by using a polyphasic approach. strain ds-18(t) grew optimally at ph 6.5-7.0 and 25 degrees c without nacl. phylogenetic analysis based on 16s rrna gene sequences showed that strain ds-18(t) belonged to the genus brevundimonas. strain ds-18(t) contained q-10 as the predominant ubiquinone and c(18 : 1)omega7c and c(16 : 0) as the maj ... | 2007 | 17911289 |
| [cloning and expression of variants of the glutaryl-7-aminocephalosporic acid acylase of the bacterium brevundimonas diminuta in escherichia coli cells]. | the gene coding for glutaryl-7-aminocephalosporic acid acylase (gl7aca acylase) of the bacterium brevundimonas diminuta (brdgl7aca), a commercial enzyme widely used in modem biocatalytic technologies for manufacture of b-lactam antibiotics, was cloned. efficient expression systems for producing a "native" recombinant brdgl7aca and its analogs modified by attaching affinity groups--the chitin-binding domain of chitinases a1 and hexahistidine sequence--were designed. it was demonstrated that both ... | 2007 | 17929575 |
| [phylogenetic diversity of culturable bacteria in the ancient salt deposits of the yipinglang salt mine, p. r. china]. | the microbial diversity of cultivable bacteria, isolated from the ancient salt deposits from the yipinglang salt mine (ypl) in the yunnan province, p. r. china,was investigated by using conventional culture-dependent method and phylogenetic analyses based on 16s rrna gene sequence comparisons. 38 bacteria strains were isolated from the brine, halite and saline soil samples on mba (marine broth agar 2216, difco) and isp 2 (international streotomyces project medium 2) media supplemented with 0.5-3 ... | 2007 | 17944352 |
| screening of rhizosphere and soil bacteria for transformability. | natural transformation is assumed to be the most likely mechanism by which dna from transgenic plants could be horizontally transferred to bacteria. in order to determine the occurrence of naturally transformable bacteria amongst bulk and rhizosphere soil bacteria, different transformation strategies were employed using either plasmid dna (incq plasmids psm1890 and psm1885, conferring gfp, sm(r), gm(r) and gfp, sm(r), tc(r), respectively) or genomic dna from rhizosphere isolates, which were chro ... | 2007 | 17961483 |
| purification and immobilization of recombinant variants of brevundimonas diminuta glutaryl-7-aminocephalosporanic acid acylase expressed in escherichia coli cells. | modified chitin-binding domain (chbd) from bacillus circulans chitinase a1 with w42f mutation in chitin-binding site was genetically fused to different positions within alpha-subunit of glutaryl-7-aminocephalosporanic acid acylase (gla) gene. hybrid proteins were efficiently expressed in e. coli cells as soluble, enzymatically active and correctly processed holoenzymes. chbd-gla fusions were easily affinity purified on chitin column by changing the salt concentration of binding and elution buffe ... | 2008 | 17963935 |
| [spatial distribution and species composition of prosthecate bacteria in lake baĭkal]. | from the water column of lake baikal, several strains of prosthecate bacteria belonging to the genera caulobacter and brevundimonas were isolated. in this article, the methods applied for their isolation and cell number determination are described; the occurrence frequency and spatial distribution of these microorganisms in the lake are demonstrated. characterization of the species composition of cultivable and uncultivable prosthecate bacteria was carried out using the methods of traditional an ... | 2007 | 17974212 |
| optimization of ascorbic acid-2-phosphate production from ascorbic acid using resting cell of brevundimonas diminuta. | with the aim to produce ascorbic acid-2-phosphate (asa-2-p) from l-ascorbic acid (asa, vitamin c), nine bacteria conferring the ability to transform asa to asa-2-p were isolated from soil samples alongside known strains from culture collections. most isolates were classified to the genus brevundimonas by 16s phylogenetic analysis. among them, brevundimonas diminuta kacc 10306 was selected as the experimental strain because of its the highest productivity of asa-2-p. the optimum set of conditions ... | 2007 | 18051298 |
| two bacterial entophytes eliciting both plant growth promotion and plant defense on pepper (capsicum annuum l.). | plant growth-promoting rhizobacteria (pgpr) have the potential to be used as microbial inoculants to reduce disease incidence and severity and to increase crop yield. some of the pgpr have been reported to be able to enter plant tissues and establish endophytic populations. here, we demonstrated an approach to screen bacterial endophytes that have the capacity to promote the growth of pepper seedlings and protect pepper plants against a bacterial pathogen. initially, out of 150 bacterial isolate ... | 2007 | 18051359 |
| filters reduce the risk of bacterial transmission from contaminated heated humidifiers used with cpap for obstructive sleep apnea. | the treatment of choice for obstructive sleep apnea (osa) is nasal continuous positive airway pressure (ncpap) during sleep, but dryness of the upper airway compromises compliance. heated humidifiers may mitigate such noncompliance; however, recent observations suggest that their use, particularly if not cleaned, increases the risk of respiratory infections. humidifier water may be contaminated, but the long-held view that passive humidifiers cannot aerosolize water may obscure the perception of ... | 2007 | 18198803 |
| chromate-tolerant bacteria for enhanced metal uptake by eichhornia crassipes (mart.). | a total of 85 chromate-resistant bacteria were isolated from the rhizosphere of water hyacinth grown in mariout lake, egypt, as well as the sediment and water of this habitat. only 4 (11%), 2 (8%), and 2 (8%) of isolates from each of the environments, respectively, were able to tolerate 200 mg cr (vi) l(-1). when these eight isolates were tested for their ability to tolerate other metals or to reduce chromate, they were shown to also be resistant to zn, mn, and pb, and to display different degre ... | 2007 | 18246718 |
| assessment of microbial diversity in effluent treatment plants by culture dependent and culture independent approaches. | microbial community structure of two distinct effluent treatment plants (etps) of pesticide and pharmaceutical industries was assessed and defined by (i) culture dependent and culture independent approaches on the basis of 16s rrna gene sequencing, (ii) diversity index analysis - operational taxonomic units (otus). a total of 38 and 44 bacterial otus having 85-99% similarity with the closest match in the database were detected among pharmaceutical and pesticide sludge samples, respectively. fift ... | 2008 | 18280146 |
| comparative phylogenies of burkholderia, ralstonia, comamonas, brevundimonas and related organisms derived from rpob, gyrb and rrs gene sequences. | phylogenetic analysis of strains from burkholderia, ralstonia, cupriavidus, comamonas, delftia, acidovorax, brevundimonas, herbaspirillum huttiense and "pseudomonas butanovora" was performed based on the protein-coding genes rpob and gyrb and on the 16s rrna-coding gene rrs. overall, the phylogenies deduced from the three genes were concordant among themselves and with current taxonomy. however, a few differences among individual gene phylogenies were noted. for example, the separation of cupria ... | 2008 | 18280706 |
| microbial community response to a release of neat ethanol onto residual hydrocarbons in a pilot-scale aquifer tank. | the microbial community response to a neat ethanol release (e100, 76 l) onto residual hydrocarbons in sandy soil was evaluated in a continuous-flow 8 m(3) pilot-scale aquifer tank, simulating a release at a bulk fuel terminal. microbial genotypic shifts were assessed using quantitative real-time pcr analysis. high ethanol concentrations in the capillary fringe at potentially toxic levels, exceeding 100,000 mg l(-1), were tolerated by the microbial community. the high biochemical oxygen demand ex ... | 2008 | 18484998 |
| biosynthesis of astaxanthin in tobacco leaves by transplastomic engineering. | summary: the natural pigment astaxanthin has attracted much attention because of its beneficial effects on human health, despite its expensive market price. in order to produce astaxanthin, transgenic plants have so far been generated through conventional genetic engineering of agrobacterium-mediated gene transfer. the results of trials have revealed that the method is far from practicable because of low yields, i.e. instead of astaxanthin, large quantities of the astaxanthin intermediates, incl ... | 2008 | 18494855 |
| comparisons of subgingival microbial profiles of refractory periodontitis, severe periodontitis, and periodontal health using the human oral microbe identification microarray. | this study compared the subgingival microbiota of subjects with refractory periodontitis (rp) to those in subjects with treatable periodontitis (grs = good responders) or periodontal health (ph) using the human oral microbe identification microarray (homim). | 2009 | 19722792 |
| colonization of overlaying water by bacteria from dry river sediments. | we studied the diversity, community composition and activity of the primary microbial colonizers of the water above freshly re-wetted sediments from a temporary river. dried sediments, collected from mulargia river (sardinia, italy), were covered with sterile freshwater in triplicate microcosms, and changes of the planktonic microbial assemblage were monitored over a 48 h period. during the first 9 h bacterial abundance was low (1.5 x 10(4) cells ml(-1)); it increased to 3.4 x 10(6) cells ml(-1) ... | 2008 | 18643927 |
| characterization of two beta-carotene ketolases, crto and crtw, by complementation analysis in escherichia coli. | the pathways from beta-carotene to astaxanthin are crucial key steps for producing astaxanthin, one of industrially useful carotenoids, in heterologous hosts. two beta-carotene ketolases (beta-carotene 4,4'-oxygenase), crto and crtw, with different structure are known up to the present. in this paper, we compared the catalytic functions of a crto ketolase that was obtained from a marine bacterium rhodococcus erythropolis strain pr4, crto derived from cyanobacterium synechosistis sp. pcc6803, and ... | 2007 | 17415558 |
| chemotaxonomy of bacteria by comprehensive gc and gc-ms in electron impact and chemical ionisation mode. | the analysis of the cellular lipidic fraction of bacteria is described. after hydrolysis and methylation, the fatty acid methyl esters (fames) are determined by 1-d gc using the sherlock midi bacteria identification system, by comprehensive gc (gc x gc) and by gc-ms in electron impact (ei) and positive chemical ionisation (pci) mode. with gc x gc, the enhanced selectivity and group type separation provides a more complete elucidation of the fatty acids in microorganisms. gc-ei-ms and gc-pci-ms w ... | 2008 | 18792008 |
| 2,2'-beta-hydroxylase (crtg) is involved in carotenogenesis of both nostoxanthin and 2-hydroxymyxol 2'-fucoside in thermosynechococcus elongatus strain bp-1. | we identified the molecular structures, including the stereochemistry, of all carotenoids in thermosynechococcus elongatus strain bp-1. the major carotenoid was beta-carotene, and its hydroxyl derivatives of (3r)-beta-cryptoxanthin, (3r,3'r)-zeaxanthin, (2r,3r,3'r)-caloxanthin and (2r,3r,2'r,3'r)-nostoxanthin were also identified. the myxol glycosides were identified as (3r,2's)-myxol 2'-fucoside and (2r,3r,2's)-2-hydroxymyxol 2'-fucoside. 2-hydroxymyxol 2'-fucoside is a novel carotenoid, and si ... | 2008 | 18794175 |
| influence of size, shape, and flexibility on bacterial passage through micropore membrane filters. | sterilization of fluids by means of microfiltration is commonly applied in research laboratories as well as in pharmaceutical and industrial processes. sterile micropore filters are subject to microbiological validation, where brevundimonas diminuta is used as a standard test organism. however, several recent reports on the ubiquitous presence of filterable bacteria in aquatic environments have cast doubt on the accuracy and validity of the standard filter-testing method. six different bacterial ... | 2008 | 18800559 |
| acclimated biomass that degrades sulfonated naphthalene formaldehyde condensate. | a number of aerobic species were isolated from textile industry activated sludge wastewater. the bacterial consortium was acclimated during seven days before testing its capacity of sulfonated naphthalene-formaldehyde condensate (snfc) recalcitrant compound degradation. snfc's degradation was evaluated by using different techniques including: vapour pressure osmometry, spectroscopy uv-visible and chemical oxygen demand (cod). the degradation of snfc by acclimated bacterial consortium was determi ... | 2008 | 18819646 |
| [degradation of hydrocarbons and their derivatives by a microbial association based on canadian pondweed]. | the degrading action of an aquatic plant-microbial association on the base of canadian pondweed (elodea canadensis) and its components (sterilized plant and two periphytonic strains, pseudomonas fluorescens e1-2.1 and brevundimonas diminuta e1-3.1) on crude oil, the water-soluble crude oil fraction, and individual test compounds (phenol, toluene, benzene, decalin, and naphthalene) was studied. it was found that the native association had a wider range and higher degree of degrading activity than ... | 2008 | 18924409 |