comparison of ec broth and medium a-1 for the recovery of escherichia coli from frozen shucked snow crab.two variations of the multiple-tube fermentation technique were used to enumerate fecal coliforms in commercially processed, frozen crab meat. these were the ec confirmation test and a more rapid method that requires medium a-1. the method with medium a-1 was more specific than the ec confirmation test for detecting escherichia coli type 1. e. coli was isolated from 84% of the positive medium a-1 tubes, whereas it was isolated from only 64% of the positive tubes of ec broth. when samples of crab ...1979384899
sarcoplasmic calcium-binding protein: identification as a new allergen of the black tiger shrimp penaeus monodon.tropomyosin and arginine kinase have been identified as crustacean allergens. during purification of arginine kinase from black tiger shrimp penaeus monodon, we found a new allergen of 20-kda.200818204275
comparative distribution of a putative egg-laying hormone in neural and reproductive tissues of four decapoda crustaceans.evidence for the presence of a putative egg-laying (elh) hormone has been previously described in the black tiger shrimp, penaeus monodon, so a further investigation was carried out to detect its presence in a range of decapoda crustaceans prior to a full molecular analysis. the crustaceans were represented by the australian fresh water yabbie, cherax destructor, the australian southern rock lobster, jasus edwardsii, the snow crab, chionoecetes opilio, and the blue swimmer crab, portunus pelagic ...200919184148
evidence of antibacterial activities in peptide fractions originating from snow crab (chionoecetes opilio) by-products.antibacterial peptide fractions generated via proteolytic processing of snow crab by-products exhibited activity against gram-negative and gram-positive bacteria. among the bacterial strains tested, peptide fractions demonstrated inhibitory activity against the gram-negative bacteria such as aeromonas caviae, aeromonas hydrophila, campylobacter jejuni, listonella anguillarum, morganella morganii, shewanella putrefasciens, vibrio parahaemolyticus and vibrio vulnificus and against a few gram-posit ...201026781242
chitin degradation proteins produced by the marine bacterium vibrio harveyi growing on different forms of chitin.relatively little is known about the number, diversity, and function of chitinases produced by bacteria, even though chitin is one of the most abundant polymers in nature. because of the importance of chitin, especially in marine environments, we examined chitin-degrading proteins in the marine bacterium vibrio harveyi. this bacterium had a higher growth rate and more chitinase activity when grown on (beta)-chitin (isolated from squid pen) than on (alpha)-chitin (isolated from snow crab), probab ...199716535505
in vitro cultivation of hematodinium sp. isolated from atlantic snow crab, chionoecetes opilio: partial characterization of late developmental stages.hematodinium is a parasitic dinoflagellate of numerous crustacean species, including the economically important atlantic snow crab, chionoecetes opilio. the parasite was cultured in vitro in modified nephrops medium at 0 °c and a partial characterization of the life stages was accomplished using light and transmission electron microscopy (tem). in haemolymph from heavily infected snow crabs two life stages were detected; amoeboid trophonts and sporonts. during in vitro cultivation, several hemat ...201525363617
characterizing changes in snow crab (chionoecetes opilio) cryptocyanin protein during molting using matrix-assisted laser desorption/ionization mass spectrometry and tandem mass spectrometry.we report the matrix-assisted laser desorption/ionization mass spectrometric (maldi-ms) characterization of the cryptocyanin proteins of the juvenile chionoecetes opilio crabs during their molting and non-molting phases. in order to assess the structural cryptocyanin protein differences between the molting and non-molting phases, the obtained peptides were sequenced by maldi low-energy collision-induced dissociation tandem mass spectrometry (cid-ms/ms).201424395503
[application of electron spin resonance spectroscopy for the detection of irradiated crustaceans (prawn, shrimp, and crabs)].prawn, shrimp and crabs sold in japan are mostly imported from overseas. detection of irradiated crustaceans is very important for quality assurance. in this study, we used esr to detect radiation-induced radicals after irradiation of prawn, shrimp and crabs of major species. no radiation-induced radicals were detected in prawn (black tiger prawn) or shrimp (white leg shrimp). radiation-induced radicals due to hydroxyapatite were detected in the claws of snow crab, red king crab, and swimming cr ...201425743382
distribution of heavy metals in muscles and internal organs of korean cephalopods and crustaceans: risk assessment for human health.samples of seven species of cephalopods and crustaceans were collected from major fish markets on the korean coast and analyzed for mercury (hg) using a direct hg analyzer and for the metals cadmium (cd), lead (pb), chromium, silver, nickel, copper, and zinc using inductively coupled plasma mass spectrometry. the distributions of heavy metals in muscles, internal organs, and whole tissues were determined, and a risk assessment was conducted to provide information concerning consumer safety. the ...201425474068
prevalence of asthma and wheezes among snow crab workers in western japan: a cross-sectional study.few reports are available concerning the prevalence of asthma among snow crab workers in japan. the object of this study was to estimate the prevalence of asthma and wheezes among japanese snow crab workers and their associations with exhaled nitric oxide (feno) and ykl-40.201627040057
modelling gastric evacuation in gadoids feeding on crustaceans.a mechanistic, prey surface-dependent model was expanded to describe the course and rate of gastric evacuation in predatory fishes feeding on crustacean prey with robust exoskeletons. this was accomplished by adding a layer of higher resistance to the digestive processes outside the inner softer parts of a prey cylinder abstraction and splitting up the prey evacuation into two stages: an initial stage where the exoskeleton is cracked and a second where the prey remains are digested and evacuated ...201627170110
lacinutrix chionocetis sp. nov., isolated from gut of a red snow crab.a gram-negative, aerobic, non-motile, rod-shaped bacterial strain, designated mab-07(t), was isolated from the gut of a red snow crab. the novel strain grew optimally at 20 °c, ph 7.0-8.0, and in the presence of 3% (w/v) nacl. a phylogenetic analysis based on the 16s rrna gene sequence indicated that the strain mab-07(t) belongs to the type strains of species of the genus lacinutrix. strain mab-07(t) exhibited 16s rrna gene sequence similarity values of 95.5-97.8% with the type strains of specie ...201628028566
simultaneous determination of two major snow crab aeroallergens in processing plants by use of isotopic dilution tandem mass spectrometry.snow crab is a major fishery in the north atlantic region. during crab processing the proteins are aerosolized and some are responsible for development of occupational asthma. tropomyosin and arginine kinase have recently been reported as major snow crab allergens. a liquid chromatographic tandem mass spectrometric method has been developed for simultaneous analysis of these two proteins in air samples collected from processing plants. these proteins were initially isolated then characterized by ...201222392376
isolation and characterization of polymorphic microsatellite markers in the red snow crab (chionoecetes japonicus).a total of 12 polymorphic microsatellite dna loci were isolated from the red snow crab, chionoecetes japonicus (brachyura: majidae), one of important fisheries resources in the far east. the number of alleles observed at each locus ranged from two to 19, with the observed and expected heterozygosities of 0.125-0.875 and 0.156-0.949, respectively, suggesting these loci to be a useful molecular marker for population analysis in this species. of the 12 loci, seven also were available for genotyping ...200821585846
concentrations of 137cs, 90sr, 108m ag, 239+240 pu and atom ratio of 240pu/239pu in tanner crabs, chionoecetes japonicus and chionoecetes opilio collected around japan.the anthropogenic radionuclides, (137)cs, (90)sr, (108m)ag, (239+240)pu, were measured in two chionoecetes species, red queen crab (chionoecetes japonicus) and snow crab (chionoecetes opilio) collected around japan during 1996-2007. there was no increase in the concentrations of these radionuclides and no large variation of the atom ratio of (240)pu/(239)pu during this research period. these results indicated that the source of the radionuclides was not the radioactive wastes dumped by the forme ...201020950831
hematodinium sp. (alveolata, syndinea) detected in marine decapod crustaceans from waters of denmark and greenland.five decapod crustacean species were examined for presence of the parasitic dinoflagellate hematodinium spp. (alveolata, syndinea) by morphological methods (colour and pleopod methods) as well as by pcr and nested pcr with hematodinium-specific primers. nephrops norvegicus, pagurus bernhardus and liocarcinus depurator were sampled by trawling in danish waters and chionoecetes opilio and hyas araneus were sampled by trapping off the west coast of greenland. the existence of hematodinium has not p ...201021166315
molecular phylogeny of a red-snow-crab species complex using mitochondrial and nuclear dna markers.nucleotide sequence variation of mitochondrial dna coi and nuclear rrna gene regions was used to reconstruct phylogenetic relationships for the red-snow-crab species complex, including the red snow crab, chionoecetes japonicus, its nominal subspecies, c. japonicus pacificus, and the triangle tanner crab, c. angulatus. the topologies of the bayesian and neighbor-joining (nj) trees of the coi and of nj trees of rrna sequences placed c. japonicus and c. angulatus in a single clade. the net sequence ...201121466347
crustacean hyperglycemic hormones of two cold water crab species, chionoecetes opilio and c. japonicus: isolation of cdna sequences and localization of chh neuropeptide in eyestalk ganglia.crustacean hyperglycemic hormone (chh) is primarily known for its prototypical function in hyperglycemia which is induced by the release of chh. the chh release takes place as an adaptive response to the energy demands of the animals experiencing stressful environmental, physiological or behavioral conditions. although >63 decapod chh nucleotide sequences are known (genbank), the majority of them is garnered from the species inhabiting shallow and warm water. in order to understand the adaptive ...201525224573
concentrations of trace metals in tissues of chionoecetes crabs (chionoecetes japonicus and chionoecetes opilio) caught from the east/japan sea waters and potential risk assessment.the concentrations of trace metals (as, cd, co, cu, fe, hg, pb, and zn) were measured in muscle and hepatopancreas of two chionoecetes crabs (chionoecetes japonicus and c. opilio) caught from the east/japan sea (ejs) in order to assess the potential health risk by the consumption of deep sea crabs. the highest metal concentrations in muscle and hepatopancreas were as and fe, respectively, while the lowest metal concentration in two tissues was pb. the mean concentrations of cd, co, cu, fe, and p ...201728303537
advances in our understanding of the global diversity and distribution of hematodinium spp. - significant pathogens of commercially exploited crustaceans.hematodinium species are parasitic dinoflagellates known to infect a growing number of marine crustacean genera from around the world, many of which support important commercial fisheries. affected hosts undergo dramatic pathological alterations to their organs, tissues and hemolymph. there are no known control measures for this disease. economically important wild fished hosts known to be susceptible to hematodinium spp. include tanner crabs chionoecetes bairdi and snow crabs chionoecetes opili ...201222433998
occupational asthma and allergy in snow crab processing in newfoundland and labrador.risk factors and prevalence of occupational asthma (oa) and occupational allergy (oal) in the snow crab-processing industry have been poorly studied.201019736174
identification of crab proteins that elicit ige reactivity in snow crab-processing workers.the expanding snow crab-processing industry has resulted in increased numbers of workers at risk of occupational allergy.200919665776
[medico-legal statistics on occupational asthmna in quebec between 1986 and 1988].the authors have carried out a statistical analysis of cases of occupational asthma and other occupational lung diseases submitted to the ministry of labour in quebec between 1986 and 1988. the total number of claims was 913, 993, and 866 respectively for the 3 years of which 61% to 71% were accepted. 41% to 55% were new assessments. of 228 new claims accepted in 1988, 81 (36%) were for occupational asthma. this number surpassed the number of claims accepted for traditional pneumoconiosis (asbes ...20002144654
sea ice retreat alters the biogeography of the bering sea continental shelf.seasonal ice cover creates a pool of cold bottom water on the eastern bering sea continental shelf each winter. the southern edge of this cold pool, which defines the ecotone between arctic and subarctic communities, has retreated approximately 230 km northward since the early 1980s. bottom trawl surveys of fish and invertebrates in the southeastern bering sea (1982-2006) show a coincident reorganization in community composition by latitude. survey catches show community-wide northward distribut ...200818488598
recovery and characterization of a serine collagenolytic extract from snow crab (chionoecetes opilio) by-products.sequential acidic precipitation followed by a single chromatographic step (gel filtration) allowed the recovery of a collagenolytic fraction containing several proteases from by-products of snow crab (chionoecetes opilio). the partial purification was particularly efficient to recover tryptic (purification fold = 1,352.5; yield = 110%) but also chymotryptic, elastolytic, and collagenolytic activities. a temperature of 40 °c and ph 8.0-8.5 were optimal for enzyme activity, which was stable for 2 ...201120853161
epidemiological determinants in outbreaks of bitter crab disease (hematodinium sp.) in snow crabs chionoecetes opilio from conception bay, newfoundland, canada.bitter crab disease (bcd) is caused by hematodinium sp., an endoparasitic dinoflagellate. it lives within the hemocoeloms of snow crabs chionoecetes opilio and tanner crabs c. bairdi, making them unmarketable due to their bitter flavor. two recent outbreaks of bcd have occurred in conception bay, newfoundland, one from 1999 to 2000 and another from 2003 to 2005. in the earlier outbreak, prevalence was highest in juvenile and primiparous females and juvenile males. it was thought to be highest in ...200717933398
comparison of different application strategies of divergicin m35 for inactivation of listeria monocytogenes in cold-smoked wild salmon.cold-smoked salmon treated with divergicin m35-producing carnobacterium divergens m35, c. divergens atcc 35677 (a non-producer of bacteriocin), purified divergicin m35 or supernatants of c. divergens m35 culture in snow crab hepatopancreas (sch) medium or mrs broth was challenged with listeria monocytogenes (up to 10(3) cfu/g). samples were stored at 4 degrees c for up to four weeks. l. monocytogenes, total bacterial and lactic acid bacterial counts were determined along with changes in total vo ...200919835762
conservation in the first internal transcribed spacer region (its1) in hematodinium species infecting crustacean hosts found in the uk and newfoundland.parasitic dinoflagellates in the genus hematodinium infect a number of decapod crustaceans in waters off the uk, including the norway lobster nephrops norvegicus and the edible crab cancer pagurus. this study investigated sequence variability in the first internal transcribed spacer (its1) region of the ribosomal rna complex of hematodinium spp. infecting n. norvegicus, c. pagurus, and pagurus bernhardus from 4 locations in the uk and from the hematodinium sp. infecting chionoecetes opilio from ...200717629120
molecular cloning of tropomyosins identified as allergens in six species of crustaceans.although tropomyosin is known to be a major allergen of crustaceans, its structural information is limited to only five species. in this study, tropomyosin was confirmed to be a major allergen in six species of crustaceans (black tiger prawn, kuruma prawn, pink shrimp, king crab, snow crab, and horsehair crab) by immunoblotting. then, the amino acid sequences of tropomyosins from these crustaceans were elucidated by a cdna cloning technique. sequence data for crustacean tropomyosins including th ...200717263503
ultrastructure of trichocysts in hematodinium spp. infecting atlantic snow crab, chionoecetes opilio.trichocyst morphology and development were explored using transmission electron microscopy in hematodinium spp. isolated directly from atlantic snow crab (chionoecetes opilio) hemolymph and from in vitro cultures. appearance of trichocysts defines the initiation of a morphological transition in the parasites life cycle from vegetative stage to the transmission stage. trichocysts within sporonts were found in distinct clusters near the nucleus in close apposition to the golgi. as cells transition ...201424975920
a review of the parasitic dinoflagellates hematodinium species and hematodinium-like infections in marine crustaceans.parasitic dinoflagellates in the genus hematodinium are important parasites of marine crustacea. outbreaks of these parasites have damaged commercial stocks of norway lobster nephrops norvegicus, snow crab chionoecetes opilio, tanner crab c. bairdi, american blue crab callinectes sapidus, and velvet swimming crab necora puber. species of hematodinium can reach high enough levels to regulate their host populations, but mortalities are also centred on the unfished juveniles and females, hosts not ...200516175968
human exposure to contaminants in the traditional greenland diet.the traditional diet is a significant source of contaminants to people in greenland, although contaminant levels vary widely among species and tissue from very low in many to very high in a few. our study has included cadmium, mercury, selenium, polychlorinated biphenyls (pcb), dichlorophenyltrichloroethane (ddt), chlordane, hexachlorocyclohexanes (hch), chlorobenzenes, dieldrin and toxaphene in the major species and tissues consumed by greenlanders. in general, the levels of these are very low ...200415325149
chitosan as an edible invisible film for quality preservation of herring and atlantic cod.the effect of chitosan with different molecular weights as coatings for shelf-life extension of fresh fillets of atlantic cod (gadus morhua) and herring (clupea harengus) was evaluated over a 12-day storage at refrigerated temperature (4 +/- 1 degrees c). three chitosan preparations from snow crab (chinoecetes opilio) processing wastes, differing in viscosities and molecular weights, were prepared; their apparent viscosities (360, 57, and 14 cp) depended on the deacetylation time (4, 10, and 20 ...200212188625
taste-active components in some foods: a review of japanese research.japanese research of taste-active components in seafoods, meat and tomatoes is reviewed. the omission (or addition) test with synthetic extracts simulating natural extracts was employed in most of that research. in abalone, sea-urchin, snow crab, scallop and short-necked clam, glutamic acid (glu) and glycine were recognized commonly to be taste-active. it was also reported that the synergism between glu and 5'-ribonucleotides and the presence of sodium and chloride ions were required to produce ...19911679558
prevalence of crab asthma in crab plant workers in newfoundland and labrador.the aim of the study was to determine the prevalence of snow crab sensitisation and occupational asthma.200415736679
population genetic analysis and origin discrimination of snow crab (chionoecetes opilio) using microsatellite markers.major habitats for the snow crab chionoecetes opilio are mostly found within the northwest atlantic and north pacific oceans. however, the east sea populations of c. opilio, along with its relative the red snow crab (c. japonicas), are two of the most important commercial crustacean species for fisheries on the east coast of the korean peninsula. the east sea populations of c. opilio are facing declining resources due to overfishing and global climate change. thus, an analysis of population stru ...201324022521
antibiotic resistance of bacteria isolated from the internal organs of edible snow crabs.antibiotic resistance and microbiota within edible snow crabs are important for the chionoecetes (snow crab) fishing industry. we investigated these parameters using culture methods and antibiotic susceptibility tests with six internal organs from three species of chionoecetes. each sample revealed many unexpected microbial species within chionoecetes internal organs. on the basis of 16s rrna sequence analysis of 381 isolates, the most abundant genera identified in chionoecetes opilio were acine ...201323990916
prey exoskeletons influence the course of gastric evacuation in atlantic cod gadus morhua.this study examined the effects of prey exoskeleton characteristics on gastric evacuation patterns in atlantic cod gadus morhua. three distinct stages were highlighted in the gastric evacuation of crustacean prey characterized by a robust exoskeleton. the experiments confirmed that the three shrimp species, pandalus borealis, pandalus montagui and eualus macilentus, and the crab chionoecetes opilio, were evacuated from the stomach at different rates. the duration of all stages increased with inc ...201323464544
snow crab allergy and asthma among greenlandic workers--a pilot study snow crab sensitization, occupational allergy and asthma in the snow crab industry in greenland, as high rates have been found in canada, but no reports have emerged from the same industry in greenland.201222901288
predicting the distribution and ecological niche of unexploited snow crab (chionoecetes opilio) populations in alaskan waters: a first open-access ensemble model.populations of the snow crab (chionoecetes opilio) are widely distributed on high-latitude continental shelves of the north pacific and north atlantic, and represent a valuable resource in both the united states and canada. in us waters, snow crabs are found throughout the arctic and sub-arctic seas surrounding alaska, north of the aleutian islands, yet commercial harvest currently focuses on the more southerly population in the bering sea. population dynamics are well-monitored in exploited are ...201121873643
aerosolized snow-crab allergens in a processing facility. 199910485396
arctic snow crab-related lung disease. 199810093350
identification of microsatellite markers in the snow crab chionoecetes opilio. 19989561792
detection of snow-crab antigens by air sampling of a snow-crab production plant.we previously assessed the prevalence of occupational asthma (oa) to snow-crab in production plant workers. we also showed that this type of oa is related to immediate immunological reactivity as demonstrated by skin reactivity and increased specific ige antibodies. however, we did not show that snow-crab antigens causing immunological reactivity and oa could be found in the air sampling in the plants atmosphere. this was the purpose of the current work.19979117885
[isolation and characteristics of collagenolytic enzymes from the hepatopancreas of the crab chionoecetes opilio]. 19911663026
impact of ultrafiltration membrane material on peptide separation from a snow crab byproduct hydrolysate by electrodialysis with ultrafiltration membranes.electrodialysis with ultrafiltration membrane (eduf) is a technology based on the separation of molecules according to their charge and molecular mass. some works have already successfully demonstrated the recovery of bioactive peptide fractions. however, the impact of ultrafiltration membrane (ufm) material, used in the eduf system, on the peptide migration has never been studied. consequently, the objectives of this work were (1) to evaluate the effect of two different ufm materials on the sel ...201121254777
ige sensitization in snow crab-processing workers.occupational asthma is a highly prevalent disease among snow crab-processing workers, but its immunologic mechanism has not been identified. prick skin tests with snow crab-meat extract, commercial extracts from other crab genera, and snow crab cooking water collected in 1984 were performed on 119 workers. crab-specific ige was assessed by rast in sera from 115 workers with meat and water extracts. both skin and rast tests were performed in 58 individuals. diagnosis of occupational asthma had pr ...19863734286
arsenobetaine in the red crab, chionoecetes opilio.the meat of the red crab, chionoecetes opilio, caught near the central coast the japan sea, was extracted with methanol. the arsenic compounds were isolated from this extract and purified by anion and cation exchange chromatography and thin-layer chromatography. three arsenic compounds were detected. the major arsenic compound, identified as arsenobetaine by tlc, proton nmr spectroscopy, and high pressure liquid chromatography with an atomic absorption spectrometer as an arsenic-specific detecto ...19863945796
biomolecular characterization of allergenic proteins in snow crab (chionoecetes opilio) and de novo sequencing of the second allergen arginine kinase using tandem mass spectrometry.snow crab (chionoecetes opilio) proteins have been recognized as an important source of both food and occupational allergens. while snow crab causes a significant occupational allergy, only one novel allergen has recently been fully characterized. the muscle proteins from snow crab legs were profiled by sds-page. several of these proteins were characterized using tandem mass spectrometry. five proteins were identified; sarcoplasmic ca-binding (20kda), arginine kinase (40), troponin (23kda) and α ...201121059421
postmortem glycolytic and nucleotide degradative changes in muscle of the atlantic queen crab (chionoecetes opilio) upon iced storage of unfrozen and of thawed meat, and upon cooking. 19751204328
absolute quantification method and validation of airborne snow crab allergen tropomyosin using tandem mass spectrometry.measuring the levels of the major airborne allergens of snow crab in the workplace is very important in studying the prevalence of crab asthma in workers. previously, snow crab tropomyosin (sctm) was identified as the major aeroallergen in crab plants and a unique signature peptide was identified for this protein. the present study advances our knowledge on aeroallergens by developing a method of quantification of airborne sctm by using isotope dilution mass spectrometry. liquid chromatography t ...201021035602
occupational asthma in the commercial fishing industry: a case series and review of the literature.we present a case series of snow crab-induced occupational asthma (oa) from a fishing and processing vessel, followed by a review of oa in the commercial fishing industry. oa is typically caused from an ige-mediated hypersensitivity reaction after respiratory exposure to aerosolized fish and shellfish proteins. it more commonly occurs due to crustaceans, but molluscs and fin fish are implicated as well. standard medical therapy for asthma may be used acutely; however, steps to reduce atmospheric ...201020496322
characterization and de novo sequencing of snow crab tropomyosin enzymatic peptides by both electrospray ionization and matrix-assisted laser desorption ionization qqtof tandem mass spectrometry.the protein tropomyosin (tm) is a known major allergen present in shellfish causing frequent food allergies. tm is also an occupational allergen generated in the working environment of snow crab (chionoecetes opilio) processing plants. the tm protein was purified from both claw and leg meats of snow crab and analyzed by electrospray ionization and matrix-assisted laser desorption/ionization (maldi) using hybrid quadruple time-of-flight tandem mass spectrometry (qqtof-ms). the native polypeptide ...201020198602
characterization of enzymatic hydrolyzed snow crab (chionoecetes opilio) by-product fractions: a source of high-valued biomolecules.snow crab (chionoecetes opilio) constitutes valuable and nutritional sources of components, such as proteins, lipids and chitin. the present investigation was undertaken to evaluate the feasibility of applying a pilot scale enzymatic hydrolysis process of snow crab by-products, followed by fractionation, in order to recover enriched high-valued compounds. the yield of snow crab by-products recovered after manual processing; on a dry weight was 87.4%. the by-products (raw materials) were mainly m ...200919286372
allergens causing occupational asthma: an evidence-based evaluation of the literature.the aim of this work is to provide an evidence-based evaluation and overview of causative substances in order to improve disease management.201423595938
a compendium of causative agents of occupational asthma.the objective is to provide an evidence-based compendium of allergenic and irritant agents that are known to cause occupational asthma in order to improve diagnostics and disease management.201323706060
determination of vitamin e and carotenoid pigments by high performance liquid chromatography in shell of chionoecetes opilio.this study reports the optimization of a method for the determination of vitamin e and carotenoids in shells of chionoecetes opilio samples by online hplc coupled with uv-vis and fluorescence detectors. the carotenoids were determined with diode-array detector (lambda 450 nm) and vitamin e with fluorescence detection (lambda(ex) 288, lambda(em) 331 nm). two extractions methods were compared, saponification followed by an extraction step and a simple extraction with acetone. the last one was sele ...200818486662
changes in embryonic development and hatching in chionoecetes opilio (snow crab) with variation in incubation temperature.water temperature affects the distribution, movement, and reproductive potential of female snow crab, chionoecetes opilio. ovigerous females of c. opilio from the eastern bering sea were held at five temperatures (-1, 0, 1, 3, and 6 degrees c) in the laboratory while their embryos developed from gastrula to hatching. the duration of incubation increased by 105 d (30%) with decreasing temperature; however, a switch to a 2-year duration of embryo incubation was not observed. for females held at 6, ...200717679721
molecular detection of hematodinium sp. in northeast pacific chionoecetes spp. and evidence of two species in the northern hemisphere.hematodinium is a genus of parasitic dinoflagellates that infects crustaceans worldwide including tanner crabs chionoecetes bairdi and snow crabs c. opilio in the northeast pacific ocean. the present study describes the optimization of a pcr-based assay for the detection and monitoring of hematodinium sp. in snow and tanner crabs. two fragments, 1682 and 187 bp, were amplified from the 18s ribosomal dna region of the parasite. the assay performed well in 6 additional decapod species (1 lobster a ...201020402233
sea urchin roe (uni) anaphylaxis.sea urchin roe, a japanese and korean delicacy served with sushi, has not been previously described as a food allergen.200717521036
seasonal and geographical variations of sterol composition in snow crab hepatopancreas and pelagic fish viscera from eastern quebec.sterol composition was determined in snow crab hepatopancreas and mackerel and herring viscera for various locations and collection periods. a simple and valuable method, using direct saponification and extraction with water-cyclohexane has been optimized to recover total sterol. they were identified and quantified as trimethylsilyl ether derivatives by gc-ms analysis. method validation indicated excellent sensitivity (limit of quantification: 1.25 mg/100 g wet basis for cholesterol and desmoste ...200717374564
pathology of hematodinium infections in snow crabs (chionoecetes opilio) from newfoundland, canada.bitter crab disease (bcd) of snow crabs, chionoecetes opilio, is caused by a parasitic dinoflagellate, hematodinium sp. the disease has shown an alarming increase in prevalence in the commercial fishery in eastern and northeastern areas of newfoundland and labrador since it was first recorded there in the early 1990s. we documented histopathological alterations to the tissues in snow crabs with heavy infections of hematodinium sp. and during sporulation of the parasite. pressure necrosis was evi ...200717336326
high-performance liquid chromatographic determination of chitin in the snow crab, chionoecetes opilio.a hplc method was developed for the determination of glucosamine in crab shells. the amount of glucosamine residue can be used to estimate the amount of chitin in analyzed material. crab samples were hydrolysed with 6m hcl at 100 degrees c for 13 h and the released glucosamine was determined by hplc using refractive index (ri) detection. separations were performed using an ods-2 column with an isocratic mobile phase consisting of 10% methanol, 90% hexanesulfonate, ph 2.1. glucosamine identificat ...200616597445
epidemiology of bitter crab disease (hematodinium sp.) in snow crabs chionoecetes opilio from newfoundland, canada.the parasitic dinoflagellate hematodinium sp. causes a condition known as bitter crab disease (bcd) in snow crabs chionoecetes opilio and tanner crabs c. bairdi. as the name of the condition implies, crabs infected with bcd are unmarketable due to their bitter flavor. we surveyed the distribution of bcd in 3 regions within the snow crab fishery of newfoundland from 1997 to 2003. over time, the disease has become firmly established in conception and bonavista bays and persists at low levels on th ...200515997824
gender and snow crab occupational asthma in newfoundland and labrador, and shellfish processing employs many thousands of people globally, with shellfish processing becoming more important in recent years. shellfish processing is associated with multiple occupational health and safety (ohs) risks. snow crab occupational asthma (oa) is work-related asthma associated with processing snow crab. we present a gender analysis of findings from a 3-year multifaceted study of snow crab oa in newfoundland and labrador, canada. the study was carried out in four snow crab ...200616109398
predation regulation of sedimentary faunal structure: potential effects of a fishery-induced switch in predators in a newfoundland sub-arctic fjord.the collapse of the cod fishery in newfoundland has coincided with marked increases in abundances of snow crab, pandalid shrimp, and other crustaceans that prey on sedimentary infauna. a 3-year sampling program in bonne bay, newfoundland indicates differences in composition and number of these predators in the two main arms of the fjord that coincide with strong differences in benthic community structure. to test whether predation pressure contributes to the observed patterns in sedimentary faun ...200515791429
knowledge, attitudes and beliefs regarding crab asthma in four communities of newfoundlad and labrador.this study was conducted to learn from snow crab plants workers and others involved in the industry their knowledge and beliefs of health issues and potential solutions.200415736680
chlorobenzenes in snow crab (chionoectes opilio): time-series monitoring following an accidental release. 200314567581
monitoring the prevalence of the parasitic dinoflagellate hematodinium sp. in snow crabs chionoecetes opilio from conception bay, newfoundland.bitter crab disease (bcd) of snow crabs chionoecetes opilio is caused by a parasitic dinoflagellate, hematodinium sp. in newfoundland's commercial fishery, infected snow crabs are identified using visual, macroscopic signs of disease for separation prior to processing. we estimated the sensitivity and specificity of gross, macroscopic diagnosis of hematodinium sp. by comparing these results with microscopic examination of prepared hemolymph smears. the sensitivity of a diagnostic test is the pro ...200312608571
variable mate-guarding time and sperm allocation by male snow crabs (chionoecetes opilio) in response to sexual competition, and their impact on the mating success of females.two laboratory experiments investigated mate guarding and sperm allocation patterns of adult males with virgin females of the snow crab, chionoecetes opilio, in relation to sex ratio. although females outnumbered males in treatments, operational sex ratios were male-biased because females mature asynchronously and have a limited period of sexual attractiveness after their maturity molt. males guarded females significantly longer as the sex ratio increased: the mean time per female was 2.9 d in a ...200111687392
biochemical indicators of muscle growth in the snow crab chionoecetes opilio (o. fabricius).this study examined the relationships between muscle growth rate, the activity of metabolic enzymes and the rna:dna ratio, in adult snow crabs chionoecetes opilio. after moulting, crabs were assigned to three feeding rations to attain a range of tissue growth rates. muscle growth rate, estimated by the variation in dry tissue content per ml of merus of the first walking leg, was positively correlated with changes in muscle cell number, as evaluated by the dna content per ml of merus. however, no ...200011090851
patterns of improvement in spirometry, bronchial hyperresponsiveness, and specific ige antibody levels after cessation of exposure in occupational asthma caused by snow-crab processing.thirty-one workers with occupational asthma caused by snow-crab processing were assessed by a long-term follow-up on three occasions at mean +/- sd intervals of 12.8 +/- 5.4, 31.4 +/- 6.3, and 64.4 +/- 6.3 months after leaving work. the diagnosis of work-related asthma was initially confirmed in all of them by specific inhalation challenges at the workplace or by laboratory inhalation of snow-crab boiling water (n = 24) or by serial monitoring of airway caliber and bronchial responsiveness to hi ...19883202454
occupational asthma in snow crab-processing workers.the prevalence of occupational asthma was studied in two snow crab-processing industries in operation since 1980. before the 1982 season, all except 10 of the 313 employees were investigated by a questionnaire, prick skin tests with common allergens, crab n and crab-boiling water extracts, and spirometry. the diagnosis was confirmed in 46 (15.6%) workers (including 33 of 64 subjects with a history highly suggestive of occupational asthma in the previous seasons) by (1) specific inhalation challe ...19846470360
effect of eyestalk-ablation on circulating ecdysteroids in hemolymph of snow crabs, chionoecetes opilio: physiological evidence for a terminal molt.bering sea snow crabs (chionoecetes opilio) are a commercially important crab harvested in the bering sea. optimal management of this species requires an understanding of the biology of this crab that is currently incomplete. fisheries managers apply a continuous growth model in their management of snow crab, which assumes that male crabs increase in size throughout their lifespan. male snow crabs undergo a morphometric molt that leads to a disproportionate increase in chelae size and it is stil ...200521676758
impact of disease on the survival of three commercially fished species.recent increases in emergent infectious diseases have raised concerns about the sustainability of some marine species. the complexity and expense of studying diseases in marine systems often dictate that conservation and management decisions are made without quantitative data on population-level impacts of disease. mark-recapture is a powerful, underutilized, tool for calculating impacts of disease on population size and structure, even in the absence of etiological information. we applied logis ...201728675580
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