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the specificity of bacterial siderophore receptors probed by bioassays.the ability to utilize siderophores of bacterial and fungal origin has been studied in wild-type and mutant strains of the enterobacterial genera salmonella, escherichia, shigella, moellerella, klebsiella, enterobacter, hafnia, pantoea, ewingella, tatumella, yersinia, and in the non-enterics aeromonas, pseudomonas and aureobacterium. although only a few representative strains were tested, the results show characteristic genus-specific differences in the utilization of hydroxamate and catecholate ...19911663779
isolation of ewingella americana from a patient with conjunctivitis.ewingella americana (family enterobacteriaceae) was isolated separately from both eyes of a 30-year-old woman. clinical signs and symptoms included adhesive eyelids, itching and edematous upper and lower lids. therapy with amoxicillin-clavulanate resulted in the relief of symptoms. ewingella americana can be isolated worldwide, but seems to be a rare pathogen.19911794367
plasmids as epidemiologic markers in nosocomial gram-negative bacilli: experience at a university and review of the literature.bacterial plasmids have become valuable markers for the comparison of strains of nosocomial gram-negative bacilli. the importance of plasmids in nosocomial infections is primarily due to their transferable antibiotic resistance genes (r plasmids), but other plasmid-mediated traits may eventually serve as potential markers. stable cryptic plasmids have also served to relate outbreak strains, particularly nonfermenting strains of gram-negative bacteria. klebsiella pneumoniae and serratia marcescen ...19863538313
wound colonization by ewingella americana.ewingella americana was recovered from a wound on the left leg of a 46-year-old male after a compound fracture of the tibia and fibula. compared with the reported characteristics of 44 american strains, this strain was shown to belong to biogroup 1. the isolation of this bacterium in south africa confirms its wide geographical distribution in clinical specimens. colonization was not associated with clinical deterioration.19863958154
mechanism of cross-contamination of blood culture bottles in outbreaks of pseudobacteremia associated with nonsterile blood collection tubes.several studies have identified nonsterile blood collection tubes as the reservoir for contaminating microorganisms in outbreaks of pseudobacteremia. microorganisms are thought to be transferred from the nonsterile tubes to the blood culture tubes when blood is drawn for cultures, but the validity of this hypothesis has not previously been tested. we recently investigated an outbreak of ewingella americana pseudobacteremia in which an epidemiological association was found between pseudobacteremi ...19854019740
ewingella americana gen.nov., sp.nov., a new enterobacteriaceae isolated from clinical specimens.we propose the name ewingella gen.nov. for a new group in the enterobacteriaceae. ewingella is phenotypically distinct from all other groups of enterobacteriaceae. the members of this genus are lipase- and deoxyribonuclease-negative; voges-proskauer-positive; lysine-, ornithine- and arginine-decarboxylase-negative; anaerogenic; they produce acid from glucose in the presence (and absence) of iodoacetate, but fail to produce acid from l-arabinose, melibiose, raffinose, d-sorbitol or sucrose. dna-r ...19836847036
isolation of ewingella americana from mollusks.twenty-three of 2446 strains of enterobacteriaceae isolated from mollusks were identified as ewingella americana both biochemically and by dna hybridization with strain s6/1111. the biochemical characteristics of the new strains showed few differences from previously reported strains obtained from human clinical specimens. these are the first strains of e. americana isolated from animals.19957580799
isolation of ewingella americana from the cultivated mushroom, agaricus bisporus.the isolation of ewingella americana, an unusual enterobacteriaceae, is reported here for the first time in a non-animal reservoir. thirty-five strains of e. americana have been recovered from the cultivated mushroom, agaricus bisporus. the biochemical characteristics of these strains are consistent with previously published descriptions of this species recovered from clinical specimens and from molluscs. dna reassociation analysis was used to confirm the identity of mushroom-derived e. american ...19968875916
catheter-related bacteremia due to ewingella americana. 200111298155
intervening sequences in rrl genes and fragmentation of 23s rrna in genera of the family enterobacteriaceae.intervening sequences (ivss) in the rrl genes for 23s rrna are transcribed but later removed by rnase iii without religation during rna processing, leading to fragmented rrna. we examined about 240 strains of the family enterobacteriaceae for presence of ivss using pcr. no ivss were detected in strains belonging to escherichia, shigella, enterobacter, erwinia, ewingella, hafnia, kluyvera, morganella, pantoea, or serratia. previously unreported ivss were detected in klebsiella oxytoca, citrobacte ...200111544246
multi-drug resistant ewingella americana.we report a case of pneumonia due to multi-drug resistant ewingella americana in a young patient admitted in the intensive care unit of hera general hospital, makkah, saudi arabia with severe head injury in a road traffic accident. he was an indonesian pilgrim who had traveled to the kingdom of saudi arabia to perform hajj in december 2007. ewingella americana was identified to be the pathogen of pneumonia with clinical signs and symptoms along with positive radiological findings.200818626541
coliform accumulation in amphibalanus amphitrite (darwin, 1854) (cirripedia) and its use as an organic pollution bioindicator in the estuarine area of recife, pernambuco, brazil.samples of water and barnacles amphibalanus amphitrite were collected from recife, brazil, to assess if it accumulates total (tc) and thermotolerant coliforms (ttc) related with sewage pollution. the most probable number (mpn) values and the standard procedures for examination of shellfish were used. comparatively with the water samples, the highest coliform values came from the barnacles, with tc values ranging from < 3.0 x 10(3) to > 2.4 x 10(6) mpn.g-1, and ttc ranging from > 2.4 x 10(3) to 2 ...201020549061
presence of specific symbiotic bacteria in flies of the subfamily tephritinae (diptera tephritidae) and their phylogenetic relationships: proposal of 'candidatus stammerula tephritidis'.the presence of symbiotic bacteria in flies belonging to the subfamily tephritinae, which predominantly infest the flower heads of composite flowers (asteraceae), was investigated. twenty-five species of flies, collected mainly in northern italy, were examined. the bacteria adhered to the midgut epithelium in a space external to the peritrophic membrane and therefore not in direct contact with the gut contents. specific, unique and live, but unculturable bacteria were consistently found in the m ...200818523165
multidrug-resistant ewingella americana: a case report and review of the literature.to describe and report a case of multidrug-resistant ewingella americana associated with exacerbation of chronic obstructive pulmonary disease (copd).200717956962
possible involvement of psychrotolerant enterobacteriaceae in blown pack spoilage of vacuum-packaged raw meats.recent investigations of blown pack spoilage in new zealand chilled vacuum-packaged meats have found moderate to high numbers of enterobacteriaceae in the spoilage flora, but no clostridia, such as c. estertheticum and c. gasigenes, that are usually associated with blown pack spoilage. this study showed that pyschrotolerant enterobacteriaceae produced gas in a lamb homogenate model under anaerobic conditions and that these organisms could cause blown pack spoilage of vacuum-packaged chilled meat ...200717870199
the general secretory pathway of burkholderia gladioli pv. agaricicola bg164r is necessary for cavity disease in white button mushrooms.cavity disease in white button mushrooms is caused by burkholderia gladioli pv. agaricicola. we describe the isolation and characterization of six mutants of the strain bg164r that no longer cause this disease on mushrooms. the mutations were mapped to genes of the general secretory pathway (gsp). this is the first report of the association of the type ii secretion pathway with a disease in mushrooms. phenotypes of the six avirulent mutants were the following: an inability to degrade mushroom ti ...200616672503
a case of pneumonia caused by ewingella americana in a patient with chronic renal failure.though the pathogenic significance and the reservoir of ewingella americana have not been clarified, this organism has caused several pathogenic infections, especially in immunocompromised patients. we report a pneumonia in a patient with chronic renal failure, who had chronic rejection of transplanted kidney. e. americana was identified to be the pathogen of pneumonia with clinical symptoms and signs and radiological examination. as soon as he was treated with ceftriaxone and isepamicin, clinic ...200515716620
natural antibiotic susceptibility of ewingella americana strains.the natural susceptibility of 20 ewingella americana strains to 72 antibiotics was examined. mic values were determined using a microdilution procedure in cation-adjusted mueller-hinton broth. evaluation of natural antibiotic susceptibility was performed applying the german standard (where applicable). beta-lactamases were examined with a conventional nitrocefin colony testing procedure, activity and induction assays, and sds-page. ewingella strains were naturally resistant or of intermediate su ...200314598935
fatal waterhouse-friderichsen syndrome due to ewingella americana infection.a fatal case of waterhouse-friderichsen syndrome resulting from infection in a previously healthy 74-year-old woman is reported. the patient died suddenly within 14 hours after presentation. the diagnosis of waterhouse-friderichsen syndrome as the cause of death was established post mortem based on autopsy findings, microscopic examination, measurement of serum procalcitonin concentration (113 ng/ml), and outcome of postmortem bacteriologic cultures that grew in heart and spleen blood samples. s ...200312604997
evaluation of the id 32e for the identification of gram-negative glucose-fermenting and glucose-non-fermenting bacilli.objective: to evaluate the id 32e bacterial identification system for accuracy in the identification of members of the family enterobacteriaceae, pseudomonas aeruginosa, stenotrophomonas maltophilia, and acinetobacter baumannii/iwoffii. methods: stock cultures of 497 enterobacteriaceae and 27 commonly encountered non-enteric gram-negative rods were tested in the id 32e system. for each isolate, the resulting 11-digit profile number was converted to an identification using the apilab plus softwar ...199911856267
a case of keratoconjunctivitis due to ewingella americana and a review of unusual organisms causing external eye infections.we report the isolation of ewingella americana from the conjunctivae of a 38 year old female physician with keratoconjunctivitis associated with the use of soft contact lens. the patient was treated successfully with topical ciprofloxacin. the source of the infection remains unknown. all contact lens cleaning materials used by the patient were sterile. since the patient was a physician, and this organism has been recorded as a cause of nosocomial infections, we checked whether cases of ewingella ...200011063558
the phylogenetic position of serratia, buttiauxella and some other genera of the family enterobacteriaceae.the phylogenetic relationships of the type strains of 38 species from 15 genera of the family enterobacteriaceae were investigated by comparative 16s rdna analysis. several sequences of strains from the genera citrobacter, erwinia, pantoea, proteus, rahnella and serratia, analysed in this study, have been analysed previously. however, as the sequences of this study differ slightly from the published ones, they were included in the analysis. of the 23 enterobacterial genera included in an overvie ...199910555323
case of peritonitis caused by ewingella americana in a patient undergoing continuous ambulatory peritoneal dialysis.reports of serious infections caused by ewingella americana have been rare. a case of e. americana peritonitis in a patient receiving continuous ambulatory peritoneal dialysis is described. this is the first report of e. americana causing such an infection.199910523587
plasmodium falciparum: inhibition of sporogonic development in anopheles stephensi by gram-negative bacteria.we investigated the effects of bacteria on plasmodium falciparum sporogonic development in anopheles stephensi. four gram-negative (escherichia coli h243, e. coli hb101, pseudomonas aeruginosa, and ewingella americana) and two gram-positive (staphylococcus aureus and staphylococcus epidermidis) bacterial strains were used in the study. tenfold dilutions of bacteria suspended in phosphate-buffered saline were mixed with an infectious meal of gametocyte-enriched cultures and fed to adult mosquitoe ...19938375488
polymicrobial bacteremia caused by ewingella americana (family enterobacteriaceae) and an unusual pseudomonas species.ewingella americana and a pseudomonas species were isolated from three sets of blood cultures from a 41-year-old patient after coronary bypass surgery. this is the first well-described case of bacteremia due to e. americana. based on data from 31 strains, a detailed description of e. americana is given.19836630449
nosocomial ewingella americana bacteremia in an intensive care unit.between dec 8, 1982, and jan 29, 1983, four cases of primary bacteremia with ewingella americana occurred in the intensive care unit of a community hospital. the patients developed high fever and leukocytosis, which gradually resolved after institution of antibiotic therapy. all infected patients had undergone either cardiovascular or peripheral vascular surgery. epidemiologic investigation revealed that the probable source for the ewingella outbreak was contamination of the ice bath used to coo ...19863942442
plasmids of ewingella americana: supplementary epidemiologic markers in an outbreak of pseudobacteremia.during an outbreak of pseudobacteremia in a children's hospital, ewingella americana was found in blood cultures from 20 patients. e. americana was inoculated into blood culture bottles at the time of specimen collection due to cross contamination from nonsterile, citrated blood collection tubes used for coagulation studies. antimicrobial susceptibility testing and plasmid profiling were used to assess the association between patient isolates and isolates from unused blood collection tubes. all ...19873571455
ewingella americana: recurrent pseudobacteremia from a persistent environmental reservoir.from september 1981 through april 1984, 20 patients at one hospital were identified with ewingella americana pseudobacteremia. case-control studies demonstrated an association between having a positive blood culture for e. americana and having blood for culture obtained simultaneously with blood obtained for coagulation studies (15 of 19 case patients versus 4 of 38 controls; p = 4.5 x 10(-7)). review of blood-drawing procedures showed that blood for coagulation studies and culture was drawn wit ...19873571454
septicemia with ewingella americana.ewingella americana was isolated from two blood cultures from a 75-year-old male after cholecystectomy. the characteristics of this strain are compared with the reported biochemical characteristics of 44 american strains. previously described infections and pseudoinfections with e. americana are reviewed.19921400980
[Observation by transmission electron microscope and identification of endophytic bacteria isolated from Bursaphelenchus xylophilus and B. mucronatus].The pine wood nematode, Bursaphlenchus xylophilus, morphologically similar to B. mucronatus, is the pathogen of pine wilt disease. This study was focused on the endophytic bacteria present in these nematodes.201122097772
isolation, selection and characterization of root-associated growth promoting bacteria in brazil pine (araucaria angustifolia).araucaria angustifolia, a unique species of this genus that occurs naturally in brazil, has a high socio-economic and environmental value and is critically endangered of extinction, since it has been submitted to intense predatory exploitation during the last century. root-associated bacteria from a. angustifolia were isolated, selected and characterized for their biotechnological potential of growth promotion and biocontrol of plant pathogenic fungi. ninety-seven strains were isolated and subje ...201121596540
prevalence of ewingella americana in retail fresh cultivated mushrooms (agaricus bisporus, lentinula edodes and pleurotus ostreatus) in zaragoza (spain).the prevalence and mycopathogenic potential of enterobacteriaceae (especially ewingella americana) in cultivated mushrooms were studied. a total of 95 samples of agaricus bisporus, lentinula edodes and pleurotus ostreatus were analyzed to quantify the enterobacteriaceae and to identify the species isolated. the host pathogenicity test was used to verify their mycopathogenic potential. the genus pseudomonas was also quantified, since it is the predominant bacterial group in cultivated mushrooms. ...200419712317
enterobacteriaceae facilitate the anaerobic degradation of glucose by a forest soil.anoxic micro zones that occur in soil aggregates of oxic soils may be temporarily extended after rainfall and thus facilitate the anaerobic degradation of organic compounds in soils. the microbial degradation of glucose by anoxic slurries of a forest soil yielded acetate, co2, h2, succinate, and ethanol, products indicative of mixed acid fermentation. prokaryotes involved in this process were identified by time-resolved 16s rrna gene-targeted stable isotope probing with [13c-u]-glucose. all labe ...200919453494
prevalence of antibiotic-resistant bacteria in herbal products.the objective of this study was to determine the prevalence of antibiotic-resistant bacteria in various herbal products. twenty-nine herbal supplements (18 traditional and 11 organic products) were purchased from stores and analyzed microbiologically. total bacterial counts were determined by pour plate and surface spreading on tryptic soy agar (tsa). antibiotic-resistant bacteria were enumerated on tsa supplemented with ceftriaxone (64 microg/ml) or tetracycline (16 microg/ml). total bacterial ...200818680952
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