| purification and properties of purine nucleoside phosphorylase from brevibacterium acetylicum atcc 954. | purine nucleoside phosphorylase of brevibacterium acetylicum atcc 954, which catalyzes the production of ribavirin (1-beta-d-ribofuranosyl-1,2,4-triazole-3-carboxamide), a potent antiviral agent, from purine nucleoside and 1,2,4-triazole-3-carboxamide in a high yield, was purified 49-fold. this enzyme had a molecular weight of 31,000 and was a monomer. the isoelectric point of the enzyme was 4.7. the optimal temperature and ph of inosine phosphorolyzing reaction catalyzed by the enzyme was aroun ... | 1991 | 1368697 |
| cellular fatty acid composition of oerskovia species, cdc coryneform groups a-3, a-4, a-5, corynebacterium aquaticum, listeria denitrificans and brevibacterium acetylicum. | twenty four strains representing eight species of gram positive yellow-pigmented rods (oerskovia turbata, oerskovia xanthineolytica, cdc coryneform groups a-3, a-4, a-5, listeria denitrificans, corynebacterium aquaticum and brevibacterium acetylicum) were divided into two major groups based on the relative amounts of 12 methyltetradecanoate (15:0a) obtained by capillary gas liquid chromatography. o. turbata, o. xanthineolytica, cdc groups a-3 and a-4, l. denitrificans and c. aquaticum were place ... | 1990 | 2124793 |
| phylogenetic interrelationships of round-spore-forming bacilli containing cell walls based on lysine and the non-spore-forming genera caryophanon, exiguobacterium, kurthia, and planococcus. | the 16s rrna gene sequences of "bacillus aminovorans" and several species considered to be phylogenetically related to the group 2 bacilli of ash et al. (c. ash, j. a. e. farrow, s. wallbanks, and m. d. collins, lett. appl. microbiol. 13:202-206, 1991) were determined. a comparative analysis of the sequence data revealed that the round-spore-forming group 2 bacilli, together with some asporogenous taxa (the genera caryophanon, exiguobacterium, kurthia, planococcus), form a phylogenetically disti ... | 1994 | 8123563 |
| characterization of guanosine kinase from brevibacterium acetylicum atcc 953. | guanosine kinase (gkase) activity was identified in a cell-free extract of brevibacterium acetylicum atcc 953. we have purified the enzyme 4000-fold from the cell-free extract to apparent homogeneity. molecular weight of 71,300 and 36,300 determined by gel filtration and sodium dodecyl sulfate gel electrophoresis, respectively, revealed that the enzyme is a dimer molecule. maximum activity of the gkase was attained when the magnesium/atp concentration ratio was close to 1. the gkase activity was ... | 1997 | 9357959 |
| molecular cloning and transcriptional analysis of a guanosine kinase gene of brevibacterium acetylicum atcc 953. | the brevibacterium acetylicum gsk gene, which encodes guanosine kinase (atp:guanosine 5'-phosphotransferase), a kinase that is involved in guanosine salvage pathways, has been cloned by using the n-terminal amino acid sequence of the purified protein. the cloned chromosomal fragment containing the gsk gene was sequenced and shown to encode a polypeptide of 303 amino acids with a molecular mass of 32,536 da, which is in good agreement with the measured molecular weight of the purified enzyme. rec ... | 1997 | 9371440 |
| molecular characterization of guanosine kinase gene from a facultative alkalophile, exiguobacterium aurantiacum atcc 35652. | guanosine kinase activity was identified in an alkalophile, exiguobacterium aurantiacum atcc 35652, and the gsk gene encoding it was isolated as a homologous fragment of the brevibacterium acetylicum gsk. the cloned chromosomal fragment contained an open reading frame which encodes a polypeptide of 308 amino acids with a molecular mass of 33349 da. the ex. aurantiacum gsk shows strong sequence similarity to b. acetylicum gsk, but possesses five additional amino acids at the c-terminus. an mr 392 ... | 1998 | 9804993 |
| development of an improved assay for purine nucleoside kinase activity in cell extracts and detection of inosine kinase activity in brevibacterium acetylicum atcc 953, related species, and corynebacterium flaccumfaciens atcc 6887. | an improved assay was developed to detect direct purine nucleoside phosphorylating activity in cell-free extracts. direct inosine phosphorylating activity was detected in 2 of 70 species tested. both activities, which depended on magnesium ion and atp, phosphorylated a hydroxyl group at the 5' position of inosine. the new assay was shown to be useful for screening of direct purine nucleoside phosphorylating activity and have the potential to detect inosine kinase in the presence of a background ... | 2000 | 10830490 |
| phosphorylation of guanosine using guanosine-inosine kinase from exiguobacterium acetylicum coupled with atp regeneration. | guanosine 5'-monophosphate (5'-gmp) and inosine 5'-monophosphate (5'-imp) are widely used as flavor enhancers. recently, a novel process for 5'-imp production by phosphorylation of inosine using guanosine-inosine kinase coupled with atp regeneration was reported. in this study, we demonstrated the practical possibility of producing 5'-gmp by phosphorylation of guanosine using a guanosine-inosine kinase from exiguobacterium acetylicum coupled with atp regeneration. | 2000 | 11129609 |
| exiguobacterium undae sp. nov. and exiguobacterium antarcticum sp. nov. | four orange-pigmented strains from pond water (l1-l4) have been subjected to polyphasic taxonomic analyses. on the basis of ribotype analysis and fourier-transform infrared spectroscopy, these strains form a genomically highly related group. 16s rdna sequence analysis revealed 98.8% similarity between the 16s rdna sequences of strains l2t and h2t, isolated previously from a microbial mat from lake fryxell, antarctica. dna-dna reassociation values indicated the presence of two genomic clusters. w ... | 2002 | 12148624 |
| exiguobacterium indicum sp. nov., a psychrophilic bacterium from the hamta glacier of the himalayan mountain ranges of india. | strain hhs 31(t), a gram-positive, motile, rod-shaped, non-spore-forming, alkaliphilic bacterium, was isolated from the melt water of a glacier. phenotypic and chemotaxonomic characteristics indicate that strain hhs 31(t) is related to species of the genus exiguobacterium. the 16s rrna gene sequence similarities between hhs 31(t) and strains of known species confirm that it is closely related to members of the genus exiguobacterium (93-99 %) and that it exhibits >97 % similarity with exiguobacte ... | 2006 | 17158975 |
| molecular identification of exiguobacterium acetylicum as the aetiological agent of bacteraemia. | a case of catheter-related bacteraemia caused by exiguobacterium acetylicum is reported in an elderly patient. the availability of sequence-based methods facilitated rapid identification and expanded the spectrum of diseases attributed to coryneform bacteria and specifically to the genus exiguobacterium. | 2007 | 17374901 |
| bioremoval of hexavalent chromium from water by a salt tolerant bacterium, exiguobacterium sp. gs1. | pollution of terrestrial surfaces and aquatic systems by hexavalent chromium, cr(vi), is a worldwide public health problem. a chromium resistant bacterial isolate identified as exiguobacterium sp. gs1 by 16s rrna gene sequencing displayed high rate of removal of cr(vi) from water. exiguobacterium sp. gs1 is 99% identical to exiguobacterium acetylicum. the isolate significantly removed cr(vi) at both high and low concentrations (1-200 microg ml(-1)) within 12 h. the michaelis-menten k ( m ) and v ... | 2008 | 18663504 |
| exiguobacterium soli sp. nov., a psychrophilic bacterium from the mcmurdo dry valleys, antarctica. | a novel psychrophilic bacterium, designated strain dvs 3y(t), was isolated from a moraine sample from the mcmurdo dry valleys, antarctica. phenotypic and chemotaxonomic characteristics and data from a phylogenetic analysis based on 16s rrna gene sequences indicated that strain dvs 3y(t) was related to the genus exiguobacterium. strain dvs 3y(t) exhibited >97 % gene sequence similarity with respect to exiguobacterium acetylicum dsm 20416(t) (97.4 %), exiguobacterium oxidotolerans dsm 17272(t) (97 ... | 2008 | 18842873 |
| efficient decomposition of shrimp shell waste using bacillus cereus and exiguobacterium acetylicum. | two bacterial cultures were isolated and tested for degradation of shrimp shell waste. according to morphological examination, physiological tests, and applied molecular techniques, isolates were identified as bacillus cereus and exiguobacterium acetylicum. both strains were cultivated separately in flasks with 100 ml of shrimp shell waste broth (3% of washed, dried and ground shrimp shell waste in tap water, ph 7.0) at 37 degrees c. at determined periods of time, deproteinization and deminerali ... | 2009 | 19471983 |
| biological activity studies on metal complexes of novel tridentate schiff base ligand. spectroscopic and thermal characterization. | metal complexes of novel schiff base (hl) ligand, prepared via condensation of 4-aminoantipyrine and 2-aminophenol, are prepared. the ligand is characterized based on elemental analysis, mass, ir and (1)h nmr spectra. metal complexes are reported and characterized based on elemental analyses, ir, (1)h nmr, solid reflectance, magnetic moment, molar conductance, esr spectra and thermal analyses (tg, dtg and dta). from the elemental analyses, 1:1 [m]:[ligand] complexes are prepared with the general ... | 2009 | 19758728 |
| molecular and phylogenetic analysis of pyridoxal phosphate-dependent acyltransferase of exiguobacterium acetylicum. | the pyridoxal-5'-phosphate (plp)-dependent family of enzymes is a very diverse group of proteins that metabolize small molecules like amino acids and sugars, and synthesize cofactors for other metabolic pathways through transamination, decarboxylation, racemization, and substitution reactions. in this study we employed degenerated primer-based pcr amplification, using genomic dna isolated from the soil bacterium exiguobacterium acetylicum strain sn as template. we revealed the presence of a plp- ... | 2009 | 20158163 |
| degradation of norgestrel by bacteria from activated sludge: comparison to progesterone. | natural and synthetic progestagens in the environment have become a concern due to their adverse effects on aquatic organisms. laboratory studies were performed to investigate aerobic biodegradation of norgestrel by bacteria from activated sludge in comparison with progesterone, and to identify their degradation products and biotransformation pathways. the degradation of norgestrel followed first order reaction kinetics (t1/2 = 12.5 d), while progesterone followed zero order reaction kinetics (t ... | 2013 | 23952780 |
| indigenous cellulolytic and hemicellulolytic bacteria enhanced rapid co-composting of lignocellulose oil palm empty fruit bunch with palm oil mill effluent anaerobic sludge. | the composting of lignocellulosic oil palm empty fruit bunch (opefb) with continuous addition of palm oil mill (pome) anaerobic sludge which contained nutrients and indigenous microbes was studied. in comparison to the conventional opefb composting which took 60-90 days, the rapid composting in this study can be completed in 40 days with final c/n ratio of 12.4 and nitrogen (2.5%), phosphorus (1.4%), and potassium (2.8%), respectively. twenty-seven cellulolytic bacterial strains of which 23 stra ... | 2013 | 24012093 |
| co-metabolism of thiocyanate and free cyanide by exiguobacterium acetylicum and bacillus marisflavi under alkaline conditions. | the continuous discharge of cyanide-containing effluents to the environment has necessitated for the development of environmentally benign treatment processes that would result in complete detoxification of the cyanide-containing wastewaters, without producing additional environmental toxicants. since biological detoxification of hazardous chemical compounds has been renowned for its robustness and environmental-friendliness, the ability of the exiguobacterium acetylicum (genbank accession numbe ... | 2016 | 28330245 |
| gene cloning and enzymatic characterization of alkali-tolerant type i pullulanase from exiguobacterium acetylicum. | a pullulanase gene (pul3yh5) of 2568 bp, which encodes a protein containing 855 amino acid residues, was cloned from the alkaliphilic bacterium exiguobacterium acetylicum yh5. the pullulanase (pul3yh5) contains the ynwgydp motif of type i pullulanase as well as four conserved glycoside hydrolase sequences of the gh13 (α-amylase) family. when the pullulanase gene was cloned and expressed in escherichia coli bl21 (de3) plyss, the recombinant pullulanase had a molecular mass of ˜100·0 kda. it was o ... | 2015 | 25273816 |
| genomic sequencing-based mutational enrichment analysis identifies motility genes in a genetically intractable gut microbe. | a major roadblock to understanding how microbes in the gastrointestinal tract colonize and influence the physiology of their hosts is our inability to genetically manipulate new bacterial species and experimentally assess the function of their genes. we describe the application of population-based genomic sequencing after chemical mutagenesis to map bacterial genes responsible for motility in exiguobacterium acetylicum, a representative intestinal firmicutes bacterium that is intractable to mole ... | 2016 | 27911803 |
| exiguobacterium enclense sp. nov., isolated from sediment. | a gram-stain-positive bacterium, designated strain nio-1109(t), was isolated from a marine sediment sample from chorao island, goa, india. phenotypic and chemotaxonomic characteristics and data from phylogenetic analysis based on 16s rrna gene sequences indicated that strain nio-1109(t) was related to the genus exiguobacterium . strain nio-1109(t) exhibited >98.0% 16s rrna gene sequence similarity with respect to exiguobacterium indicum hhs 31(t) (99.5%) and exiguobacterium acetylicum ncimb 9889 ... | 2015 | 25716950 |
| growth promotion of wheat seedlings by exiguobacterium acetylicum 1p (mtcc 8707) a cold tolerant bacterial strain from the uttarakhand himalayas. | exiguobacterium acetylicum strain 1p (mtcc 8707) is a gram-positive, rod-shaped, yellow pigmented bacterium isolated from soil on nutrient agar plates at 4°c. the identity of the bacterium was arrived on the basis of the biochemical characterization, biolog sugar utilization pattern and sequencing of the 16s rrna gene. it grew at temperatures ranging from 4 to 42°c, with temperature optima at 30°c. it expressed multiple plant growth promotion attributes such as phosphate solubilization, indole a ... | 2010 | 23100807 |
| investigation of gut microbial communities associated with indigenous honey bee (apis mellifera jemenitica) from two different eco-regions of saudi arabia. | the microbial communities associated with the alimentary tract of honey bees are very important as they help with food digestion, provide essential nutrients, protect the host from pathogens, detoxify harmful molecules, and increase host immunity. in this study, the structural diversity of the gut microbial communities of native honey bees, apis mellifera jemenitica from two different geographical regions (riyadh and al-baha) of saudi arabia was analyzed by culture-dependent methods and 16s ribo ... | 2017 | 28663705 |