| antigens of aspergillus fumigatus. iii. comparative immunochemical analyses of clinically relevant aspergilli and related fungal taxa. | cell sap (cs) of the pathogenic fungus aspergillus fumigatus strain ag-507 was fractionated by sephadex g-200 column chromatography. a protein fraction designated cs3 was partially characterized by two dimensional electrophoresis (2-de) and analytical ultracentrifugation. cs3 consisted mainly of low molecular weight components (14 k-43 k) of the whole cs, and produced one peak in analytical ultracentrifugation with an sapp of 4.25. cs3 was demonstrated to be different from a previously character ... | 1985 | 3921301 |
| polyene-resistant mutants of aspergillus fennelliae: identification of sterols. | four mutants strains of aspergillus fennelliae resistant to various polyene antibiotics have been analyzed for their sterol content. the mutant strains contained delta(7,22,24(28))-ergostatrien-3beta-o1 and delta(7,22)-ergostadien-3beta-o1 as the major sterols, whereas the wild types contained ergosterol, indicating that the mutants contain metabolic blocks for c(5)-c(6) dehydrogenation and c(24)-c(28) reduction in the biosynthesis of ergosterol. revertant sectors arising from the mutant colonie ... | 1974 | 4157334 |
| utility of random amplified polymorphic dna pcr and taqman automated detection in molecular identification of aspergillus fumigatus. | we developed a method for the identification of aspergillus fumigatus fungal isolates by using random amplified polymorphic dna (rapd) pcr (rapd-pcr) cloning and the taqman ls50b fluorogenic detection system (perkin-elmer corp., applied biosystems, foster city, calif.). dna from seven clinically important aspergillus species was screened by rapd-pcr to identify section- or species-specific amplicons. with the opz19 rapd primer a 1,264-bp product was amplified from all a. fumigatus strains initia ... | 1998 | 9650962 |
| polyene-resistant mutants of aspergillus fennelliae: sterol content and genetics. | mutants of aspergillus fennelliae (neosartorya fennelliae) resistant to relatively high levels of amphotericin b and low levels of nystatin were obtained by successive transfers of wild type in the presence of increasing concentrations of the polyenes. the resistance of the mutants to the polyenes was accompanied by both qualitative and quantitative changes in the sterol composition of the cells. those resistant to amphotericin b (af5-ab1 and p-ab1) lacked ergosterol, the major sterol of the wil ... | 1974 | 15828178 |
| a new dihydrochromone dimer and other secondary metabolites from cultures of the marine sponge-associated fungi neosartorya fennelliae kufa 0811 and neosartorya tsunodae kufc 9213. | a previously unreported dihydrochromone dimer, paecilin e (1), was isolated, together with eleven known compounds: β-sitostenone, ergosta-4,6,8 (14), 22-tetraen-3-one, cyathisterone, byssochlamic acid, dehydromevalonic acid lactone, chevalone b, aszonalenin, dankasterone a (2), helvolic acid, secalonic acid a and fellutanine a, from the culture filtrate extract of the marine sponge-associated fungus neosartorya fennelliae kufa 0811. nine previously reported metabolites, including a chromanol der ... | 2017 | 29194412 |