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epidemiologic evaluation of the risk factors associated with exposure and seroreactivity to bartonella vinsonii in dogs.to determine seroprevalence to bartonella vinsonii subsp berkhoffii in a population of sick dogs from north carolina and virginia and to evaluate potential risk factors associated with increased likelihood of exposure to the organism.19979140552
sequential evaluation of dogs naturally infected with ehrlichia canis, ehrlichia chaffeensis, ehrlichia equi, ehrlichia ewingii, or bartonella vinsonii.historically, disease manifestations in dogs seroreactive to ehrlichia canis antigens by indirect immunofluorescent antibody testing have been attributed to infection with either e. canis or ehrlichia ewingii. a 1996 study by dawson and colleagues provided pcr evidence that healthy dogs from southeastern virginia could be naturally infected with ehrlichia chaffeensis. this observation stimulated us to determine which ehrlichia spp. infected sick dogs that were referred to our hospital from the s ...19989705408
seroepidemiology of bartonella vinsonii subsp. berkhoffii infection in california coyotes, 1994-1998.the prevalence of antibodies to bartonella vinsonii subsp. berkhoffii in coyotes (canis latrans) in california ranged from 51% in central to 34% in southern and 7% in northern california. seropositive coyotes were more likely to be from coastal than inland counties (p clustered distribution of bartonella seropositivity in coyotes suggests that b. vinsonii subsp. berkhoffii infection is vectorborne. further investigation is warranted to evaluate which arthropods are vectors and what the mode of t ...199910511529
bartonella vinsonii subsp. berkhoffii and related members of the alpha subdivision of the proteobacteria in dogs with cardiac arrhythmias, endocarditis, or myocarditis.cardiac arrhythmias, endocarditis, or myocarditis was identified in 12 dogs, of which 11 were seroreactive to bartonella vinsonii subspecies berkhoffii antigens. historical abnormalities were highly variable but frequently included substantial weight loss, syncope, collapse, or sudden death. fever was an infrequently detected abnormality. cardiac disease was diagnosed following an illness of short duration in most dogs, but a protracted illness of at least 6 months' duration was reported for fou ...199910523564
bartonella vinsonii subsp. berkhoffii as an agent of afebrile blood culture-negative endocarditis in a human.we report a case of endocarditis in a human infected with bartonella vinsonii subsp. berkhoffii, which causes bacteremia and endocarditis in dogs. bacterial identification was established by pcr amplification and sequencing of an intergenic spacer region (its1), 16s ribosomal dna, and a gene encoding citrate synthase (glta). bartonella antibodies were detected by immunofluorescence.200010747175
cyclic cd8+ lymphopenia in dogs experimentally infected with bartonella vinsonii subsp. berkhoffii.until recently, it was presumed that bartonella vinsonii only infected voles, a species of north american rodents. in april of 1993, however, our laboratory isolated a novel subspecies of b. vinsonii (b. vinsonii subsp. berkhoffii) from the blood of a dog diagnosed with vegetative valvular endocarditis. subsequently, based on a seroepidemiologic survey of dogs from north carolina and virginia presenting for a variety of medical problems, we found evidence supporting a potentially important assoc ...200010889298
coyotes (canis latrans) as the reservoir for a human pathogenic bartonella sp.: molecular epidemiology of bartonella vinsonii subsp. berkhoffii infection in coyotes from central coastal california.bartonella vinsonii subsp. berkhoffii was originally isolated from a dog suffering infectious endocarditis and was recently identified as a zoonotic agent causing human endocarditis. following the coyote bite of a child who developed clinical signs compatible with bartonella infection in santa clara county, calif., this epidemiological study was conducted. among 109 coyotes (canis latrans) from central coastal california, 31 animals (28%) were found to be bacteremic with b. vinsonii subsp. berkh ...200011060089
assessing the association between the geographic distribution of deer ticks and seropositivity rates to various tick-transmitted disease organisms in dogs.to determine whether the geographic distribution of deer ticks (ixodes scapularis) was associated with the distribution of dogs seropositive for various tick-transmitted disease organisms (ie, borrelia burgdorferi, rickettsia rickettsii, the human granulocytic ehrlichiosis [hge] agent, ehrlichia canis, and bartonella vinsonii subsp berkhoffii).200111318358
seroepidemiology of bartonella vinsonii subsp berkhoffii exposure among healthy dogs.to determine seroprevalence of antibodies to bartonella vinsonii subsp berkhoffii and risk factors for seropositivity among working dogs owned by the us government.200111518174
antinuclear antibodies can be detected in dog sera reactive to bartonella vinsonii subsp. berkhoffii, ehrlichia canis, or leishmania infantum antigens.the presence of antinuclear antibodies (anas) is used to support a clinical diagnosis of systemic lupus erythematosus (sle) in dogs. however, clinicians must interpret the detection of anas with caution, particularly in light of increasing evidence that dogs with known bacterial and protozoal infections can have high ana titers. retrospectively, medical records were reviewed for all dogs that were concurrently tested for antinuclear antigens and bartonella vinsonii (berkhoffii), ehrlichia canis, ...200414765731
seroprevalence of antibodies against bartonella species and evaluation of risk factors and clinical signs associated with seropositivity in dogs.to determine the seroprevalence of antibodies against bartonella spp in a population of sick dogs from northern california and identify potential risk factors and clinical signs associated with seropositivity.200515900951
environmental factors associated with bartonella vinsonii subsp. berkhoffii seropositivity in free-ranging coyotes from northern california.bartonella vinsonii subsp. berkhoffii is a newly recognized pathogen of domestic dogs and humans. coyotes (canis latrans) are considered an important reservoir of this bacterium in the western united states, but its vectors are still unknown. our objective was to identify environmental factors associated with bartonella antibody prevalence in 239 coyotes from northern california, using an enzyme-linked immunosorbent assay. in addition, associations were evaluated between b. v. berkhoffii and two ...200516011426
isolation of bacteriophages from bartonella vinsonii subsp. berkhoffii and the characterization of pap31 gene sequences from bacterial and phage dna.bacteriophages enhance bacterial survival, facilitate bacterial adaptation to new environmental conditions, assist in the adaptation to a new host species, and enhance bacterial evasion or inactivation of host defense mechanisms. we describe the detection and purification of a novel tailed bacteriophage from bartonella vinsonii subsp. berkhoffii, which was previously described as a bacteriophage-negative species. we also compare b. vinsonii subsp. berkhoffi pap31 bacteriophage gene sequences to ...200516254445
a serological study of exposure to arthropod-borne pathogens in dogs from northeastern spain.there is limited information regarding the prevalence of many vector borne pathogens in europe and especially in spanish dogs. we investigated 206 sick and 260 clinically healthy dogs from three different regions in northeastern spain for antibodies to rickettsia conorii (rc), ehrlichia canis (ec), anaplasma phagocytophilum (ap), bartonella henselae (bh), bartonella vinsonii subsp. berkhoffii (bvb), leishmania infantum (li) and borrelia burgdorferi (bb) and for antigen of dirofilaria immitis (di ...200616472522
antibodies to bartonella vinsonii subsp. berkhoffii in moroccan dogs.bartonella vinsonii subsp. berkhoffii is a fastidious microorganism that has been recognized as an emerging human and canine pathogen. we report for the first time on the prevalence of antibodies to b. vinsonii subsp. berkhoffii in domestic dogs from morocco. the overall seroprevalence was 38% (56 of 147 dogs tested). most of the seropositive dogs were stray dogs from rabat (36%, 8 of 22) and khenifra (47%, 47 of 101). antibodies against b. vinsonii subsp. berkhoffii were found infrequently amon ...200616474074
canine bartonellosis: serological and molecular prevalence in brazil and evidence of co-infection with bartonella henselae and bartonella vinsonii subsp. berkhoffii.the purpose of this study was to determine the serological and molecular prevalence of bartonella spp. infection in a sick dog population from brazil. at the são paulo state university veterinary teaching hospital in botucatu, 198 consecutive dogs with clinicopathological abnormalities consistent with tick-borne infections were sampled. antibodies to bartonella henselae and bartonella vinsonii subsp. berkhoffii were detected in 2.0% (4/197) and 1.5% (3/197) of the dogs, respectively. using 16s-2 ...200717583666
bartonella vinsonii subsp. berkhoffii endocarditis in a dog from saskatchewan.a dog referred for lameness was diagnosed with culture-negative endocarditis. antibodies to bartonella spp. were detected. antibiotic treatment resulted in transient clinical improvement, but the dog developed cardiac failure and was euthanized. bartonella vinsonii subsp. berkhoffii genotype iv was identified within the aortic heart valve lesions by pcr amplification and dna sequencing.200717824328
prevalence of ehrlichia canis, anaplasma platys, babesia canis vogeli, hepatozoon canis, bartonella vinsonii berkhoffii, and rickettsia spp. in dogs from grenada.to identify the tick-borne pathogens in dogs from grenada, we conducted a serologic survey for ehrlichia canis in 2004 (104 dogs) and a comprehensive serologic and molecular survey for a variety of tick-borne pathogens in 2006 (73 dogs). in 2004 and 2006, 44 and 32 dogs (42.3% and 43.8%) were seropositive for e. canis, respectively. in 2006, several tick-borne pathogens were identified by serology and pcr. dna of e. canis, anaplasma platys, babesia canis vogeli, hepatozoon canis, and bartonella ...200818160223
molecular characterization of bartonella vinsonii subsp. berkhoffii genotype iii.the molecular characterization of a bartonella vinsonii subsp. berkhoffii genotype iii strain (ncsu strain 06-co1) isolated from the blood of a military working dog diagnosed with endocarditis is reported in this study. several genes were amplified and sequenced for comparative sequence similarity with other strains.200818367567
molecular evidence for bartonella spp. in cat and dog fleas from germany and france.nine hundred and fifty-two fleas were collected from 148 cats and 133 dogs at 18 widely distributed geographic locations in germany and france and examined for the presence of six different bartonella spp. (bartonella bacilliformis, bartonella clarridgeiae, bartonella elizabethae, bartonella henselae, bartonella quintana, bartonella vinsonii subsp. berkhoffii) by pcr. thirty-five specimens (3.7%) tested positive for either b. henselae (14 positive fleas) or b. clarridgeiae (21 positive fleas). d ...200818489542
failure to identify an association between serologic or molecular evidence of bartonella infection and idiopathic rhinitis in dogs.to determine whether infection with or exposure to bartonella spp was associated with idiopathic rhinitis in dogs.200818710315
seroprevalence of bartonella spp. in the endangered island fox (urocyon littoralis).bartonella clarridgeiae-like strains, presently b. rochalimae, were isolated in gray foxes (urocyon cinereoargenteus) in mainland california. the objective of this study was to investigate the presence of bartonella infection in the endangered island fox (urocyon littoralis) found only on the channel islands off the californian coast. between 2001 and 2004, 263 serum samples were collected. antibodies against bartonella vinsonii subsp. berkhoffii (bvb) and b. clarridgeiae (bc) were detected usin ...200919058928
isolation or molecular detection of bartonella henselae and bartonella vinsonii subsp. berkhoffii from dogs with idiopathic cavitary effusions. 200919175739
molecular documentation of bartonella infection in dogs in greece and italy.this study reports the occurrence of "bartonella rochalimae" in europe and the presence of bartonella vinsonii subsp. berkhoffii genotypes ii and iii in dogs in southern italy and provides dna sequencing evidence of a potentially new bartonella sp. infecting dogs in greece and italy.200919261798
isolation of bartonella vinsonii subsp. berkhoffii genotype ii from a boy with epithelioid hemangioendothelioma and a dog with hemangiopericytoma.in this report, we describe isolation of bartonella vinsonii subsp. berkhoffii genotype ii from a boy with epithelioid hemangioendothelioma and a dog with hemangiopericytoma. these results suggest that b. vinsonii subsp. berkhoffii may cause vasoproliferative lesions in both humans and dogs.200919369441
recurrent osteomyelitis in a cat due to infection with bartonella vinsonii subsp. berkhoffii genotype ii. 200919709358
evaluation of brain tissue or cerebrospinal fluid with broadly reactive polymerase chain reaction for ehrlichia, anaplasma, spotted fever group rickettsia, bartonella, and borrelia species in canine neurological diseases (109 cases).vector-transmitted microorganisms in the genera ehrlichia, anaplasma, rickettsia, bartonella, and borrelia are commonly suspected in dogs with meningoencephalomyelitis (mem), but the prevalence of these pathogens in brain tissue and cerebrospinal fluid (csf) of dogs with mem is unknown.201020102497
bartonella vinsonii subsp. berkhoffii and bartonella henselae bacteremia in a father and daughter with neurological disease.abstract:201020377863
molecular evidence of perinatal transmission of bartonella vinsonii subsp. berkhoffii and bartonella henselae to a child.bartonella vinsonii subsp. berkhoffii, bartonella henselae, or dna of both organisms was amplified and sequenced from blood, enrichment blood cultures, or autopsy tissues from four family members. historical and microbiological results support perinatal transmission of bartonella species in this family. it is of clinical relevance that bartonella spp. may adversely influence human reproductive performance.201020392912
seroprevalence of bartonella vinsonii subsp. berkhoffii in urban and rural dogs in turkey.the seroprevalence of bartonella vinsonii subsp. berkhoffii was investigated in stray urban dogs and shepherd and farm guard dogs from rural areas sampled from 10 provinces of turkey. sera from 855 dogs were examined for the presence of anti-b. vinsonii subsp. berkhoffii antibodies by indirect fluorescent antibody test. overall, 56 (6.6%) of the 855 dogs examined, including 16 (3%) of the 522 stray dogs and 40 (12%) of the 333 rural dogs, were seropositive. this is the first report on prevalence ...201020574140
molecular evidence of bartonella infection in domestic dogs from algeria, north africa, by polymerase chain reaction (pcr).bartonella species are being recognized as important bacterial human and canine pathogens, and are associated with multiple arthropod vectors. bartonella dna extracted from blood samples was obtained from domestic dogs in algiers, algeria. polymerase chain reaction (pcr) and dna sequence analyses of the ftsz gene and the 16s-23s intergenic spacer region (its) were performed. three bartonella species: bartonella vinsonii subsp. berkhoffii, bartonella clarridgeiae, and bartonells elizabethae were ...201020682871
isolation or detection of bartonella vinsonii subspecies berkhoffii and bartonella rochalimae in the endangered island foxes (urocyon littoralis).bartonella rochalimae (b.r.) and bartonella vinsonii subsp. berkhoffii (b.v.b.) have been isolated from gray foxes (urocyon cinereoargenteus) in mainland california and high bartonella seroprevalence was reported in island foxes (u. litorralis), especially from santa cruz and santa rosa islands. as a follow-up study, the objectives were to determine the prevalence of bartonella bacteremia and seropositivity and to identify the bartonella species infecting a convenience sample of 51 island foxes ...201121802869
bartonella spp. dna in cardiac tissues from dogs in colorado and wyoming.background: several bartonella species (spp.) have been identified in dogs diagnosed with infectious endocarditis (ie) or myocarditis. objective: to interrogate cardiac tissues of dogs with suspected ie for the presence of bartonella spp. dna of dogs in the rocky mountain states. animals: nine dogs with a clinical diagnosis of endocarditis from january 1990 to june 2008 were included. methods: in this retrospective study, medical records at the veterinary teaching hospital were searched. animals ...201121539606
pcr amplification of bartonella koehlerae from human blood and enrichment blood cultures.abstract:201020735840
bacillary angiomatosis in an immunosuppressed dog.abstract a dog being treated with immunosuppressive doses of prednisone and azathioprine for pancytopenia of unknown origin, developed, over a 2-week period, multiple erythematous nodular lesions in the skin including footpads. skin samples revealed lesions identical to those of human bacillary angiomatosis (ba). the nodules were composed of multifocal proliferations of capillaries, each lined by protuberant endothelial cells. the capillary clusters were separated by an oedematous connective tis ...201020374571
detection and genetic diversity of bartonella vinsonii subsp. berkhoffii strains isolated from dogs in ankara, turkey.bartonella vinsonii subsp. berkhoffii has been identified as an important pathogen in dogs and is an emerging pathogen in people with zoonotic potential. this study aimed to isolate bartonella spp. in 250 blood samples collected from dogs in the province of ankara, turkey, between october 2006 and march 2007. the typing of the 23 isolates was carried out by pcr for citrate synthase (glta) and the 16s-23s intergenic transcribed spacer (its). the prevalence of bacteremia was 9.2% in 250 samples. a ...201020234114
co-isolation of bartonella henselae and bartonella vinsonii subsp. berkhoffii from blood, joint and subcutaneous seroma fluids from two naturally infected dogs.this report describes the clinical presentation, isolation and treatment of two dogs naturally infected with bartonella henselae and bartonella vinsonii subsp. berkhoffii. chronic and progressive polyarthritis was the primary complaint for dog #1, from which b. henselae and b. vinsonii subsp. berkhoffii were cultured on three independent occasions from blood and joint fluid samples, despite administration of nearly 4 months of non-consecutive antibiotic therapy. a clinically atypical and progres ...200919560291
molecular characterization of the sucb gene encoding the immunogenic dihydrolipoamide succinyltransferase protein of bartonella vinsonii subsp. berkhoffii and bartonella quintana.members of the genus bartonella have historically been connected with human disease, such as cat scratch disease, trench fever, and carrion's disease, and recently have been recognized as emerging pathogens causing other clinical manifestations in humans. however, because little is known about the antigens that elicit antibody production in response to bartonella infections, this project was undertaken to identify and molecularly characterize these immunogens. immunologic screening of a bartonel ...200312874367
spatial analysis of yersinia pestis and bartonella vinsonii subsp. berkhoffii seroprevalence in california coyotes (canis latrans).zoonotic transmission of sylvatic plague caused by yersinia pestis occurs in california, usa. human infections with various bartonella species have been reported recently. coyotes (canis latrans) are ubiquitous throughout california and can become infected with both bacterial agents, making the species useful for surveillance purposes. this study examined the geographic distribution of 863 coyotes tested for y. pestis and bartonella vinsonii subsp. berkhoffii serologic status to gain insight int ...200312507856
genetic classification and differentiation of bartonella species based on comparison of partial ftsz gene sequences.currently, 19 species are recognized in the genus bartonella, 7 of which are involved in an increasing variety of human diseases. development of molecular tools for detection, identification, and subtyping of strains and isolates has promoted research on bartonella spp. we amplified and sequenced the portion of the ftsz gene encoding the n-terminal region of the cell division protein for 13 bartonella species: bartonella alsatica, b. birtlesii, b. doshiae, b. elizabethae, b. grahami, b. koehlera ...200212354859
granulomatous disease associated with bartonella infection in 2 dogs.shortly after removal of an engorged tick from the left ear, a 4-year-old greyhound was referred for evaluation of fever and a rapidly enlarging mass in the region of the left submandibular lymph node. histopathologic evaluation of the lymph node resulted in a diagnosis of severe granulomatous lymphadenitis. an 11-year-old mixed-breed dog was referred for evaluation of a 6-week history of serous nasal discharge. histologic examination of a surgical biopsy from a nasal mass indicated multifocal g ...200010668815
bartonella vinsonii subsp. berkhoffii subsp. nov., isolated from dogs; bartonella vinsonii subsp. vinsonii; and emended description of bartonella vinsonii.two bacterial strains, one isolated from the blood of a dog with valvular endocarditis and one isolated from the blood of a healthy dog, were similar to bartonella species, as determined by a number of phenotypic criteria, including growth characteristics, biochemical reactions, and cell wall fatty acid composition. the results of 16s rrna gene sequence similarity studies confirmed that these strains are closely related and belong in the genus bartonella and that bartonella vinsonii is their clo ...19968782679
invasion of canine erythrocytes by bartonella vinsonii subsp. berkhoffii.bartonella vinsonii subsp. berkhoffii is a recognized cause of endocarditis in dogs and human patients and has been associated with cardiac arrhythmias, myocarditis, granulomatous lymphadenitis, polyarthritis, and granulomatous rhinitis in dogs. little is known regarding the mode of transmission or cellular localization of this bacteria following infection of a canine host. the aim of the current study was to determine whether erythrocytes may serve as a site of infection by b. vinsonii subsp. b ...201122071179
ecological diversity of bartonella species infection among dogs and their owner in virginia.abstract bartonella species comprise a genus of gram-negative, fastidious, intracellular bacteria that have been implicated in association with an increasing spectrum of disease manifestations in dogs and human patients. in this study, chronic canine and human disease, for which causation was not diagnostically defined, were reported by the breeder of a kennel of doberman pinschers. in addition to other diagnostic tests, serology, polymerase chain reaction, and enrichment blood culture were used ...201121736485
molecular and serological diagnosis of bartonella infection in 61 dogs from the united states.background: molecular diagnosis of canine bartonellosis can be extremely challenging and often requires the use of an enrichment culture approach followed by pcr amplification of bacterial dna. hypotheses: (1) the use of enrichment culture with pcr will increase molecular detection of bacteremia and will expand the diversity of bartonella species detected. (2) serological testing for bartonella henselae and bartonella vinsonii subsp. berkhoffii does not correlate with documentation of bacteremia ...201121615498
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