dna fingerprinting in domestic animals using four different minisatellite probes.four probes known to allow dna fingerprinting in the human (m13, jeffreys' core sequence, the human alpha globin hypervariable region [hvr], and a mouse probe related to the drosophila per gene) were checked for their ability to reveal "genetic bar codes" in cattle, horses, pigs, dogs, chickens, and a european cyprinid fish, the barbel (barbus barbus l.). individual-specific patterns were obtained in cattle using m13, jeffreys' core sequence, and the alpha globin hvr, in horses, dogs, and pigs u ...19883163967
[study of communities of monogenea dactylogyridae parasites of the cyprinidae in lake mikri prespa (northern greece). description of 3 new species from an endemic barbus: barbus cyclolepis prespensis karaman, 1924].in this paper are described 17 species of monogenea dactylogyridae, parasites of cyprinidae from lake mikri prespa, in greece: 14 species have been already reported in mediterranean region; 3 new species have been harvested on an endemic barbus, b. cyclolepis prespensis, dactylogyrus balkanicus n. sp., dactylogyrus crivellius n. sp., dactylogyrus prespensis n. sp. for these communities of dactylogyrus, we discuss some problems of parasite specificity, speciation and biogeography. from the morpho ...19863566079
colonization and establishment of pomphorhynchus laevis (acanthocephala) in an isolated english river.the successful colonization, establishment and spread of pomphorhynchus laevis in a small, isolated, devon river 128 km from the parasite's nearest focus in dorset was followed over 11 years from 1985. the parasite was first detected in anguilla anguilla and platichthys flesus in 1988: by 1995 it had attained prevalence levels of 22.6% in a. anguilla and 43.6% in p. flesus and also occurred in 100% salmo trutta, 50% cottus gobio and noemacheilus barbatulus. as judged by prevalence, abundance, pr ...19968991914
seasonal occurrence and maturation of neoechinorhynchus rutili (acanthocephala) in barbel, barbus barbus (pisces), of the jihlava river, czech republic.from april 1992 until june 1993, the seasonal occurrence and maturation of the acanthocephalan neoechinorhynchus rutili in its definitive host, the barbel (barbus barbus), was studied in the jihlava river (the danube basin), czech republic. the parasite occurred in barbel throughout the year (overall prevalence 84% and intensity of infection 1-533 [mean 25] acanthocephalars per fish), but there were distinct seasonal fluctuations in prevalence and mean intensity values: prevalence attained 100% ...19949140494
long-term recovery of macrobenthos and fish assemblages after water pollution abatement measures in the river petite baïse (france).riverine ecosystems are subject to a large variety of man-made influences, and in recent years a new public awareness of the need to protect rivers has emerged within the industrialized world. the present study focuses on the effect of abatement of pollution from one factory on the recovery of fish and macroinvertebrate species richness in the river petite baïse over a 20-year period (1973-1993). until 1973, the petite baïse (75 km in length) received not only factory waste (nitrogenous rich eff ...19979353205
the intestinal parasite pomphorhynchus laevis (acanthocephala) from barbel as a bioindicator for metal pollution in the danube river near budapest, hungary.concentrations of as, al, ag, ba, bi, cd, co, cr, cu, fe, ga, mg, mn, ni, pb, sb, sn, sr, tl, v and zn were analyzed by inductively coupled mass spectrometry (icp-ms) in the intestinal helminth pomphorhynchus laevis and its host barbus barbus. the fish were caught in the danube river downstream of the city of budapest (hungary). ten out of twenty one elements analyzed were found at higher concentrations in the acanthocephalan than in different tissues (muscle, intestine, liver and kidney) of bar ...200415016463
the intestinal parasite pomphorhynchus laevis as a sensitive accumulation indicator for the platinum group metals pt, pd, and rh.concentrations of the platinum group elements pt, pd, and rh were analyzed by adsorptive cathodic stripping voltammetry (pt, rh) and total-reflection x-ray fluorescence (pd) in the intestinal helminth pomphorhynchus laevis and its host barbus barbus. the fish were caught in the danube river south of the city of budapest (hungary) and were exposed to ground catalytic material for 28 days. following exposure all three precious metals were taken up and accumulated in host organs and the parasites. ...200515721887
helminth communities of the barbel barbus barbus from large river systems in austria.the composition and diversity of the total and intestinal component endohelminth communities were determined in the cyprinid barbel from three study areas in two large river systems in austria. two sample sites in the danube and one site in the river drau are the only free flowing stretches of these rivers in austria. nine helminth species were identified, with the acanthocephalan pomphorhynchus laevis being dominant in the danube, with up to 100% prevalence. in the drau, where the dominant spec ...200515946396
fish as biomonitors of polybrominated diphenyl ethers and hexabromocyclododecane in czech aquatic ecosystems: pollution of the elbe river basin.background: brominated flame retardants (bfrs)--polybrominated diphenyl ethers (pbdes) and hexabromocyclododecane (hbcd)--belong to the group of relatively "new" environmental contaminants. the occurrence of these compounds in the czech aquatic ecosystem was for the first time documented within the 3-year monitoring study initiated in 2001. in 2002-2003 hbcd and the major pbde congeners (28, 47, 49, 66, 85, 99, 100, 153, 154, and 183) were found in 136 freshwater fish samples collected from seve ...200718174947
the endohelminth fauna of barbel ( barbus barbus) correlates with water quality of the danube river in bulgaria.summary: infection of barbel with 10 species of metazoan parasites including 3 trematodes, 3 acanthocephalans and 4 nematodes was observed in fish collected from 3 localities in the bulgarian part of the river danube between summer 2004 and summer 2007. the composition as well as the diversity characteristics of the parasite communities were analysed seasonally and showed a clear correlation with the composition of the invertebrate fauna and water quality. the most prevalent species was the acan ...200919250599
chemical contaminants in fish species from rivers in the north of luxembourg: potential impact on the eurasian otter (lutra lutra).contamination levels of pcbs, and of the heavy metals cadmium (cd), lead (pb) and mercury (hg) were analyzed in four fish species from seven rivers in the north of luxembourg. during august and september 2007, 85 samples of fish were collected belonging to four species: the stone loach (barbatula barbatula, n=12 pools), the chub (squalius cephalus, n=36), the barbel (barbus barbus, n=23) and eel (anguilla anguilla, n=14). the concentration of seven indicator pcbs ( summation operator(7)pcbs) rea ...201020060148
is metal accumulation in pomphorhynchus laevis dependent on parasite sex or infrapopulation size?concentrations of the elements as, cd, co, cu, fe, mn, mo, ni, pb, sn, v, zn were analysed by inductively coupled plasma mass spectrometry (icp-ms) in the acanthocephalan pomphorhynchus laevis and its fish host barbus barbus. a total of 27 barbels were collected from the danube river in autumn 2006 close to the town kozloduy (685 river kilometer) on the bulgarian river bank. fish were divided into 3 groups. according to their p. laevis infrapopulation size hosts were considered as heavily infect ...201020380766
non-dioxin-like pcbs in ten different fish species from the danube river in serbia.this work has been developed to examine the level of non-dioxin-like (ndl) pcbs (28, 52, 101, 138, 153 and 180) in (a) ten different freshwater fish species from the danube river, (b) two sampling points: up and downstream of the industrial zone of the city of pancevo (ecological hot spot in serbia) and (c) two time points i.e., in 2001 and 2006. obtained results would serve to analyse spatial, temporal and congener profile characteristics of ndl pcbs cumulated in fish tissues due to environment ...201021161586
intestinal immune response of silurus glanis and barbus barbus naturally infected with pomphorhynchus laevis (acanthocephala).immunopathological and ultrastructural studies were conducted on the intestine of barbel barbus barbus and sheatfish silurus glanis that were naturally infected with the acanthocephalan pomphorhynchus laevis. enteric helminths often cause inflammation of the digestive tract, inducing the recruitment of different types of immune cells at the site of infection. the results of our study clearly demonstrated that mast cells (mc) were the dominant immune cells which occur at the site of inflammation ...201121226724
first detection and analysis of a fish circovirus.circoviruses are present worldwide in birds and pigs but their occurrence in fish has not been reported yet. recently, increased mortality was observed in barbel fry (barbus barbus) in hungary. the paper reports the detection of previously unknown circular viral dna genomes in barbels by the use of a circovirus specific wide range nested polymerase chain reaction. the analysis of two complete genomes (barbel circovirus, bacv1 and bacv2) indicated that they belonged into a new genetic group withi ...201121525210
first detection of pike fry-like rhabdovirus in barbel and spring viraemia of carp virus in sturgeon and pike in aquaculture in the czech republic.rapid antigen detection enzyme-linked immunosorbent assay (elisa) testing of cell cultures with organ homogenate from fish, collected from farms with a predominance of common carp or in natural aquaculture in the czech republic between 1995 and 2008, identified piscine vesiculovirus in 27 of 178 samples. using reverse transcription semi-nested pcr, targeting a 550 nucleotide region of the glycoprotein (g) gene, piscine vesiculovirus was confirmed in 23 of the 27 organ samples diagnosed by elisa ...201121848117
understanding trophic interactions in host-parasite associations using stable isotopes of carbon and nitrogen.stable isotope analysis of carbon and nitrogen can deliver insights into trophic interactions between organisms. while many studies on free-living organisms are available, the number of those focusing on trophic interactions between hosts and their associated parasites still remains scarce. in some cases information about taxa (e.g. acanthocephalans) is completely missing. additionally, available data revealed different and occasionally contrasting patterns, depending on the parasite's taxonomic ...201728212669
seasonal profile of metal accumulation in the acanthocephalan pomphorhynchus laevis: a valuable tool to study infection dynamics and implications for metal monitoring.a large number of studies demonstrated that acanthocephalans exhibit a high metal accumulation potential and thus can be used as sensitive accumulation indicators. however, similar to free-living bioindicators, a seasonal variation in metal concentrations in parasites might occur. accordingly, the influence of seasonality has to be elucidated if parasites should be applied as sentinels.201627216617
spatial monitoring of heavy metals in the inland waters of serbia: a multispecies approach based on commercial fish.the study monitored the contamination of fish muscle tissue by elements al, as, cd, co, cr, cu, fe, hg, mn, ni, pb, and zn at 17 sampling sites, in order to assess the pollution status of the main rivers in serbia. of the six commercially important fish species included in the study (pikeperch sander lucioperca, catfish silurus glanis, bream abramis brama, barbel barbus barbus, chub squalius cephalus, nase chondrostoma nasus), the bioconcentration factor (bcf) indicated that benthivore bream and ...201626857004
occurrence of intersex in wild freshwater fish in slovenian rivers: a histological evaluation.the aim of this preliminary research was to establish if there are intersex occurrences in wild freshwater fish in slovenian rivers and streams. in the first study we evaluated all fish species of both sexes obtained from the river ljubljanica from its source to mouth. in the second study we focused on the rainbow trout (oncorhynchus mykiss) and brown trout (salmo trutta m. fario) males from 30 rivers and streams in different parts of slovenia. the male gonads were histologically assessed for th ...201627749265
characterizing the trophic niches of stocked and resident cyprinid fishes: consistency in partitioning over time, space and body sizes.hatchery-reared fish are commonly stocked into freshwaters to enhance recreational angling. as these fishes are often of high trophic position and attain relatively large sizes, they potentially interact with functionally similar resident fishes and modify food-web structure. hatchery-reared barbel barbus barbus are frequently stocked to enhance riverine cyprinid fish communities in europe; these fish can survive for over 20 years and exceed 8 kg. here, their trophic consequences for resident fi ...201627547336
erratum: loss of genetic integrity and biological invasions result from stocking and introductions of barbus barbus: insights from rivers in england.[this corrects the article doi: 10.1002/ece3.1906.].201627252838
high variability in stable isotope diet-tissue discrimination factors of two omnivorous freshwater fishes in controlled ex situ discrimination factors (δ(13)c and δ(15)n) are influenced by variables including the tissues being analysed and the taxon of the consumer and its prey. whilst differences in δ(13)c and δ(15)n are apparent between herbivorous and piscivorous fishes, there is less known for omnivorous fishes that consume plant and animal material. here, the omnivorous cyprinid fishes barbus barbus and squalius cephalus were held in tank aquaria and exposed to three diets that varied in their constituen ...201626896544
loss of genetic integrity and biological invasions result from stocking and introductions of barbus barbus: insights from rivers in england.anthropogenic activities, including the intentional releases of fish for enhancing populations (stocking), are recognized as adversely impacting the adaptive potential of wild populations. here, the genetic characteristics of european barbel barbus barbus were investigated using 18 populations in england, where it is indigenous to eastern-flowing rivers and where stocking has been used to enhance these populations. invasive populations are also present in western-flowing rivers following introdu ...201626843923
massive invasion of exotic barbus barbus and introgressive hybridization with endemic barbus plebejus in northern italy: where, how and why?biological invasions and introgressive hybridization are major drivers for the decline of native freshwater fish. however, the magnitude of the problem across a native species range, the mechanisms shaping introgression as well as invader's dispersal and the relative role of biological invasions in the light of multiple environmental stressors are rarely described. here, we report extensive (n = 665) mtdna sequence and (n = 692) microsatellite genotypic data of 32 northern adriatic sites aimed t ...201324103005
interstitial movement and emergence of barbel barbus barbus free embryos and larvae.the movement of barbel barbus barbus free embryos in the interstitial zone and the timing of larval emergence were studied for 12 days in an artificial stream. after egg fertilization (day 1), hatching occurred on day 6, downstream interstitial movements of free embryos on day 9 and emergence and downstream movement of all larvae on day 12, with density of free embryos decreasing as day of emergence approached. the results suggest that a memory-based response of b. barbus to environmental condit ...201323464560
content of selected metals in muscle of cyprinid fish species from the nitra river, slovakia.this study presents a rate of bioaccumulation of some metal concentrations in the muscle of five common cyprinid fish species (gudgeon - gobio gobio, europen chub - leuciscus cephalus, barbel - barbus barbus, roach - rutilus rutilus, and nase - chondrostoma nasus).201223353849
compensatory growth, condition and food utilization in barbel barbus barbus juveniles reared at different feeding periodicities with a dry a 40 day experiment, barbel barbus barbus juveniles were fed a dry diet continuously, or food was withheld for 2, 5 or 10 days, and then resumed feeding. improved feed utilization efficiency allowed fish deprived of food for 2 or 5 days to fully compensate for body mass loss. short-term food deprivation as a method of preventing negative effects of overfeeding in intensive rearing of juvenile b. barbus is proposed.201323331156
comparison of in vitro and in situ genotoxicity in the danube river by means of the comet assay and the micronucleus test.genotoxicity can be correlated with adverse reproductive effects or may even result in elevated extinction risk for particular species of an ecosystem. it may thus be a valuable tool for screening of pollution and potential environmental harm. since many genotoxicants tend to adsorb onto particulate matter, sediments and suspended materials are of particular interest for genotoxicity screening under field conditions. in order to correlate the genotoxic potential of sediments with genetic damage ...201020417309
immunocytochemical study of cell type distribution in the pituitary of barbus barbus (teleostei, cyprinidae).antisera to mammalian pituitary and placental hormones have been used to identify and localize the different cell types in the pituitary of the barbel (barbus barbus, l.). the immunocytochemical labeling employed the immunoperoxidase technique or the immunogold silver staining procedure. corticotrophic and prolactin cells, visualized using antisera to human adrenocorticotropic hormone and ovine prolactin (prl), respectively, occur in the rostral pars distalis (rpd). antisera against mammalian go ...19911652534
occurrence of priority and emerging organic compounds in fishes from the rhone river (france).the main objective of this study was to collect new data on the occurrence, levels of priority and emerging organic compounds in freshwater fish sampled in the rhone river. the 34 studied contaminants included alkylphenols, bisphenol a, polybromodiphenylethers (pbde), perfluorinated compounds, hexabromocyclododecanes (hbcd), hexachlorobenzene and hexachlorobutadiene (hcbd). about 50 fish samples (individual specimens or pooled fish) were collected from three sites located upstream and downstream ...201222760502
assessment of fish health status in the upper danube river by investigation of ultrastructural alterations in the liver of barbel barbus barbus.despite intensive efforts and tightened guidelines for improvement of water quality over the last 2 decades, declines of fish populations have been reported for several rivers around the world. the present study forms part of a comprehensive weight-of-evidence approach, which aims to identify potential causes for the decline in fish catches observed in the upper danube river. the major focus of the present study is the investigation of the health status of wild barbel barbus barbus l. collected ...201020377013
comparison of the myxobolus fauna of common barbel from hungary and iberian barbel from portugal.we compared myxobolus infection of common barbel barbus barbus from the danube river in hungary with that in iberian barbel luciobarbus bocagei from the este river in portugal. in hungary, we recorded 5 known myxobolus species (m. branchialis, m. caudatus, m. musculi, m. squamae, and m. tauricus) and described m. branchilateralis sp. n. in portugal we recorded 6 myxobolus species (m. branchialis, m. branchilateralis sp. n., m. cutanei, m. musculi, m. pfeifferi, and m. tauricus). species found in ...201222968791
supplementation with sodium selenite and selenium-enriched microalgae biomass show varying effects on blood enzymes activities, antioxidant response, and accumulation in common barbel (barbus barbus).yearling common barbel (barbus barbus l.) were fed four purified casein-based diets for 6 weeks in outdoor cages. besides control diet, these were supplemented with 0.3 mg kg(-1) dw selenium (se) from sodium selenite, or 0.3 and 1.0 mg kg(-1) from se-enriched microalgae biomass (chlorella), a previously untested se source for fish. fish mortality, growth, se accumulation in muscle and liver, and activity of selected enzymes in blood plasma, muscle, liver, and intestine were evaluated. there was ...201424772422
physical changes in specimens of five species of cyprinidae preserved in ethanol and frozen.preservation in 30% ethanol and freezing to a temperature of -20 ± 2° c is an appropriate method for measurement of fish eggs, larvae and juveniles. egg diameter of the common carp cyprinus carpio increased insignificantly by 1·32% after preservation compared with live size. the total length (lt ) of 1 day post-hatching (dph) larvae as well as the standard length (ls) of 16 dph larvae of c. carpio increased significantly (2·95 and 1·50%, respectively) after preservation. egg diameter as well as ...201425041177
common barbel (barbus barbus) as a bioindicator of surface river sediment pollution with cu and zn in three rivers of the danube river basin in serbia.river sediments are a major source of metal contamination in aquatic food webs. due to the ability of metals to move up the food chain, fishes, occupying higher trophic levels, are considered to be good environmental indicators of metal pollution. the aim of this study was to analyze the metal content in tissues of the common barbel (barbus barbus), a rheophilous cyprinid fish widely distributed in the danube basin, in order to find out if it can be used as a bioindicator of the metal content in ...201626662100
arsenic speciation patterns in freshwater fish.muscle of 16 freshwater fish (9 different species belonging to 4 different families) was analysed for arsenic species using hplc separation (anion and cation exchange) followed by on-line uv-decomposition, hydride generation and afs detection. the main arsenic compounds found in the extracts were arsenobetaine (asb), which accounted for 92-100% of extractable arsenic in species of salmonids (salmo marmoratus, oncorhynchus mykiss, salmo trutta m. fario), and dimethylarsinic acid (dmaa), which acc ...200418969382
a spatially explicit resource-based approach for managing stream fishes in riverscapes.the article describes a riverscape approach based on landscape ecology concepts, which aims at studying the multiscale relationships between the spatial pattern of stream fish habitat patches and processes depending on fish movements. a review of the literature shows that few operational methods are available to study this relationship due to multiple methodological and practical challenges inherent to underwater environments. we illustrated the approach with literature data on a cyprinid specie ...200616456624
comparison of the effect of four anaesthetics on haematological profiles, oxidative stress and antioxidant enzymes in barbel (barbus barbus).currently, many questions regarding the effect of anaesthetics to fish remain unresolved. fish species may differ widely in their response to an anaesthetic, the screening of dosages is often necessary. the aim of this study was to compare the effect of tricaine methane sulphonate (ms 222), clove oil, 2-phenoxyethanol and propiscin on haematological profiles, oxidative stress biomarkers and antioxidant enzymes in barbel (barbus barbus).201526757110
combining geometric morphometrics with molecular genetics to investigate a putative hybrid complex: a case study with barbels barbus spp. (teleostei: cyprinidae).this integrative study examined the morphological and genetic affinities of three endemic barbel species from italy (brook barbel barbus caninus, italian barbel barbus plebejus and horse barbel barbus tyberinus) and of putative hybrid specimens to their species of origin. two of the species frequently occur together with the non-native barbel barbus barbus. dna barcoding indicates that mitochondrial (mt) haplotypes often do not match the species expected from morphology. linear distance measurem ...201626805755
metazoan parasite communities: support for the biological invasion of barbus barbus and its hybridization with the endemic barbus meridionalis.recently, human intervention enabled the introduction of barbus barbus from the rhône river basin into the barbus meridionalis habitats of the argens river. after an introduction event, parasite loss and lower infection can be expected in non-native hosts in contrast to native species. still, native species might be endangered by hybridization with the incomer and the introduction of novel parasite species. in our study, we aimed to examine metazoan parasite communities in barbus spp. population ...201627855708
permanent genetic resources added to molecular ecology resources database 1 april 2013-31 may 2013.this article documents the addition of 234 microsatellite marker loci to the molecular ecology resources database. loci were developed for the following species: acipenser sinensis, aleochara bilineata, aleochara bipustulata, barbus meridionalis, colossoma macropomum, delia radicum, drosophila nigrosparsa, fontainea picrosperma, helianthemum cinereum, liomys pictus, megabalanus azoricus, pelteobagrus vachelli, pleuragramma antarcticum, podarcis hispanica type 1a, sardinella brasiliensis and scle ...201323937578
use of histopathology and elemental accumulation in different organs of two benthophagous fish species as indicators of river pollution.the bottom feeding fish species have a good potential to be used for assessments of pollution, as they are under pressure from pollutants from both water and sediments. in this study, the level of similarity of histopathological responses to pollution in gills and liver between barbel (barbus barbus) and sterlet (acipenser ruthenus) from the danube river was assessed, and compared with elemental concentrations in their gills, liver, and muscle. results indicate that the detected metal concentrat ...201524677754
fish predation and offspring survival in the prosobranch snail viviparus this study we identified some of the predators of the freshwater snail viviparus ater and estimated offspring survival to the end of the first summer in a natural population. newborn v. ater were eaten by the fish barbus barbus, rutilus rutilus, scardinius erythrophtalmus and tinca tinca. out of 137 guts of abramis brama caught in lake zürich 1 contained shell fragments and an operculum of a newborn v. ater. on a 40×40 m grid near goldbach, lake zürich, we counted 678 adult females of v. ater ...199328313816
comparison of the metal accumulation capacity between the acanthocephalan pomphorhynchus laevis and larval nematodes of the genus eustrongylides sp. infecting barbel (barbus barbus).metal uptake and accumulation in fish parasites largely depends on the parasite group with acanthocephalans showing the highest accumulation rates. additionally, developmental stage (larvae or adult) as well as parasite location in the host are suggested to be decisive factors for metal bioconcentration in parasites. by using barbel (barbus barbus) simultaneously infected with nematode larvae in the body cavity and adult acanthocephalans in the intestine, the relative importance of all of these ...201323332036
concentration of selected metals in muscle of various fish species.the purpose of this study was to assess concentration of selected metals (fe, mn, zn, cu, ni, co, cr, pb, cd, and hg) in the muscle of four common slovak fish species (chub--leuciscus cephalus, barbel--barbus barbus, roach--rutilus rutilus, and perch--perca fluviatilis). furthermore, correlations among the selected metals and order of metal accumulation in the fish muscle were determined. an electrofishing technique was used for collecting the fish from the nitra river (slovakia) in september 20 ...200515792307
autosomal differences between males and females in hybrid zones: a first report from barbus barbus and barbus meridionalis (cyprinidae).narrow hybrid zones are generally subjected to the action of two forces: dispersal, which tends to homogenise the hybridising taxa, and selection against hybrids, which, in contrast, produces steep clines of introgression for diagnostic markers. although differences between sexes in dispersal abilities or in susceptibility to hybrid counterselection are common in hybrid zones, autosomal genetic differences between males and females have never been reported to our knowledge. barbus barbus and bar ...200415273701
myosin, parvalbumin and myofibril expression in barbel (barbus barbus l.) lateral white muscle during development.histo- and immunohistochemical techniques have recently been used to study the fibre type and myosin expression in fish muscle during development. in the present work, embryonic, larval and adult myosin isozymes (heavy and light chains) and parvalbumin isotypes were analyzed, from fertization to the adult stage, by polyacrylamide gel electrophoresis of barbel (barbus barbus l.) trunk muscle extracts. the examined myosins display the sequential transitions from embryonic to larval and adult forms ...199224214210
molecular phylogeny of congeneric monogenean parasites (dactylogyrus): a case of intrahost speciation.dactylogyrus species (dactylogyridae: monogenea) are a group of monogenean gill parasites that are highly specific to freshwater fish of the family cyprinidae. dactylogyrus species were sampled from 19 cyprinids and one percid collected in europe. using partial 18s rdna and its1 sequences, a phylogeny of 51 dactylogyrus species was reconstructed to investigate the patterns of parasite speciation and diversification. three main dactylogyrus lineages were recognized from all phylogenetic trees, th ...200415212381
life history of the nematode rhabdochona hellichi, a parasite of the barbel in the jihlava river, czech republic.from april 1992 until june 1993, the seasonal dynamics of rhabdochona hellichi (srámek, 1901) in its definitive host, the barbel (barbus barbus), was studied in the jihlava river (the danube basin), czech republic. the parasite occurred in barbel throughout the year (overall prevalence 93% and intensity of infection 1-1384 [mean 78] nematodes per fish), but there were distinct seasonal fluctuations in prevalence and mean intensity values associated with the parasite's seasonal cycle of maturatio ...19957622792
celozoic myxosporidians (myxidium spp. and chloromyxum spp.) of cyprinids from the river esla (león, nw spain). ii. population the analysis of the population dynamics of various celozoic myxosporidians found in cyprinids of the river esla (león, nw spain) a bimodal seasonality was observed for myxidium macrocapsulare auerbach, 1910, myxidium carinae alvarez-pellitero et al., 1983 and chloromyxum complicatum alvarez-pellitero et al., 1983. we did not observe a clear influence of the age and sex of the host on the infections. an apparently positive association was observed for myxidium carinae and chloromyxum complicat ...19836412598
celozoic myxosporidians (myxidium spp. and chloromyxum spp.) of cyprinids from the river esla (león, nw spain). i. description of the the examination of 417 cyprinids, belonging to 7 species, caught in the esla river from april 1977 to december 1979, the following celozoic myxosporidians were found: myxidium macrocapsulare auerbach, 1910 in leuciscus cephalus cabeda and chondrostoma polylepis polylepis; myxidium carinae n. sp. in barbus barbus bocagei; chloromyxum complicatum n. sp. in b. barbus bocagei and l. cephalus cabeda; chloromyxum chondrostomi n. sp. in ch. polylepis polylepis; and chloromyxum cyprini fujita, 1927 i ...19836859619
[structure and ultrastructure of the caudal neurosecretory system of barbus barbus gilb. and salmo irideus l]. 19724681623
[concentrating ability of the gallbladder: a comparative study in some vertebrate species (author's transl)].the bile salts, chloride, sodium, potassium and nitrogen content of the gallbladder bile was studied in goat, sheep, cattle, pig, rabbit, dog, monkey (erythrocebus patas), chicken, lizard (lacerta ocellata), frog (rana esculenta), trout (salmo gardnieri) and barbel (barbus barbus). a significant correlation was found between bile salts and sodium (positive), chloride and sodium (negative) and chloride and bile salts (negative). the gallbladder concentrating ability was very high in monkey, lizar ...1975808839
a pcr-based method for diet analysis in freshwater organisms using 18s rdna barcoding on faeces.the development of dna barcoding from faeces represents a promising method for animal diet analysis. however, current studies mainly rely on prior knowledge of prey diversity for a specific predator rather than on a range of its potential prey species. considering that the feeding behaviour of teleosts may evolve with their environment, it could prove difficult to establish an exhaustive listing of their prey. in this article, we extend the dna barcoding approach to diet analysis to allow the in ...201021564994
sperm quality in male barbus barbus l. fed different diets during the spawning season.sperm quality of barbus barbus l. was compared among the three following dietary regimes: group a, fed 100% commercial diet (karpico containing 33% crude protein and 6% fat), group b, fed 78% commercial diet and 22% frozen chironomid (chironomus plumosus) larvae, and group c, fed 56% commercial diet and 44% frozen chironomid larvae. concentrations of polyunsaturated fatty acids (pufas) in group a, b, and c were 39.1, 42.0, and 44.6, respectively, as a percentage of total fatty acids. sperm morph ...200919779852
relationship between semen characteristics and body size in barbus barbus l. (teleostei: cyprinidae) and effects of ions and osmolality on sperm motility.the objectives of the present study were to determine the relationships among length and weight of males, sperm volume, spermatozoa concentration, total number of spermatozoa, ionic contents and osmolality of seminal plasma in barbus barbus. the effect of osmolality on sperm motility parameters after activation in nacl, kcl, or sucrose solutions was also examined. there were significant correlations between spermatozoa concentration - length (r=+0.7) and - weight (r=+0.8) of males. no significan ...200919351565
rare and asymmetrical hybridization of the endemic barbus carpathicus with its widespread congener barbus endemic species that co-occur with widespread congeners, hybridization can lead to an influx of novel and beneficial genetic variation, but high rates of introgression may cause genetic swamping of the endemic species and have detrimental effects on its survival potential. this study examines hybridization between sympatric populations of the carpathian barbel barbus carpathicus, a recently discovered cryptic species with a restricted range, and the widespread common barbel barbus barbus. bas ...200920735568
morphological changes of the nucleolus during oogenesis in oviparous teleost fish, barbus barbus (l.).in fishes, like in amphibians, it is well established that variations in rrna activity occur during oogenesis. contrary to amphibians, however, little is known about the ultrastructural changes of the nucleolus during fish oogenesis. evolution of the nucleolus has been followed during oogenesis in the teleost fish barbus barbus (l.) using light and transmission electron microscopies. we show that the behaviour of the nucleolus during b. barbus oogenesis resembles that reported in amphibians but ...200516165370
thermal acclimation effects differ between voluntary, maximum, and critical swimming velocities in two cyprinid fishes.temperature acclimation may be a critical component of the locomotor physiology and ecology of ectothermic animals, particularly those living in eurythermal environments. several studies of fish report striking acclimation of biochemical and kinetic properties in isolated muscle. however, the relatively few studies of whole-animal performance report variable acclimation responses. we test the hypothesis that different types of whole-animal locomotion will respond differently to temperature accli ...200613130428
a novel functional class i lineage in zebrafish (danio rerio), carp (cyprinus carpio), and large barbus (barbus intermedius) showing an unusual conservation of the peptide binding domains.species from all major jawed vertebrate taxa possess linked polymorphic class i and ii genes located in an mhc. the bony fish are exceptional with class i and ii genes located on different linkage groups. zebrafish (danio rerio), common carp (cyprinus carpio), and barbus (barbus intermedius) represent highly divergent cyprinid genera. the genera danio and cyprinus diverged 50 million years ago, while cyprinus and barbus separated 30 million years ago. in this study, we report the first complete ...200212165519
phylogeography of the barbel (barbus barbus) assessed by mitochondrial dna variation.using the phylogeographic framework, we assessed the dna sequence variation at the mitochondrial cytochrome b gene across the distribution range of the barbel barbus barbus, a widely distributed european cyprinid. reciprocal monophyly of non-mediterranean european and balkan/anatolian populations is taken as evidence for a long-term barrier to gene flow, and interpreted as a consequence of survival of the species in two separate refugia during several later glacial cycles. lack of profound genea ...200111555260
cryopreservation of spermatozoa in cyprinid fishes.the present study investigated semen cryopreservation in cyprinid fish using computer-assisted sperm motility analysis for viability control. spermatozoa of the bleak, chalcalbumus chalcoides, were used as a basic model to describe the toxic and cryoprotective effects of internal and external cryoprotectants, their most effective concentrations and combinations, the freezing and thawing conditions, and the effects of equilibration. we also used these data to develop a cryopreservation protocol f ...200011191871
dna reassociation kinetics in diploid and phylogenetically tetraploid cyprinidae.four diploid and three phylogenetically tetraploid cyprinidae (ostariophysi) have been characterized as for nuclear dna content, modal chromosome number and dna reassociation kinetics (hydroxyapatite chromatography). among the diploid species nuclear dna content (10(-12) g dna/2c) was 1.62 for tinca tinca, 1.87 for scardinius erythrophthalmus, 2.53 for leuciscus cephalus and 2.75 for alburnus alburnus, while the phylogenetically tetraploid species carassius auratus, barbus barbus and cyprinus ca ...1979490124
branchial osteogenetic neoplasm in barbel barbus barbus plebejus.a branchial osteogenetic neoplasm affecting a barbel barbus barbus plebejus (valenciennes, 1829) is described. the osteoblasts' pleomorphism, the lack of a well-developed and complete separation, the presence of eccentric, terminal proliferative edges infiltrating the lining tissues and the abundant tumour matrix suggest a histopathological diagnosis of a 'productive osteoblastic osteosarcoma'. the occurrence of eosinophilic granule cells (egcs) scattered among neoplastic tissue is discussed in ...199910546053
fish p53 as a possible biomarker for genotoxins in the aquatic environment.the p53 gene is a tumour suppressor gene which has a fundamental role in cell cycle control and division, and in mammals certain genotoxic agents induce specific mutations in p53, leading to tumourigenesis. fish have been investigated as models for studying carcinogens, but as yet very little data exists that links exposure to specific chemicals with the aetiology of tumours found in wild populations. in this study, p53 was sequenced from five species of fish with a view to the possible use of m ...199910334619
a fine structural review on the spermatozoa of cyprinidae with attention to their phylogenetic implications.the fine structural organization and dimensions of spermatozoa from species of 4 subfamilies of the cyprinidae (barbus barbus, carassius carassius, cyprinus carpio carpio, cyprinus carpio haematopterus, abramis brama, alburnoides bipunctatus, alburnus alburnus, chalcalburnus chalcoides mento, chondrostoma nasus, hypophthalmichthys molitrix, leuciscus cephalus, phoxinus phoxinus, rutilus rutilus, rutilus meidingerii, scardinius erythrophthalmus, vimba vimba and ctenopharyngodon idella) are compar ...200919688692
does polyploidy lead to fewer and shorter microsatellites in barbus (teleostei:cyprinidae)?screening of a hybrid barbus barbus-b. meridionalis genome was performed for ca, ga, tat, tct, tag, tgt, tatt, tact, atct motifs, and simultaneously on another fish species, tilapia s. melanotheron. sequences of positive clones were obtained for barbus and revealed that repetitive structure significantly depends on the motif: most tat and tatt repeats contain small numbers of repeats, and these repeats are highly heterogeneous, whereas other motifs (we mainly obtained ca and gata repeats) form l ...19979061942
phenotypic plasticity in caryophyllaeus brachycollis janiszewska, 1953 (cestoda: caryophyllidea): does fish host play a role?recent molecular phylogenetic studies on fish tapeworms of the genus caryophyllaeus gmelin, 1790 (cestoda: caryophyllidea), parasites of cyprinid fishes in the palaearctic region, have revealed unexpected phenotypic plasticity that seems to be related to definitive hosts. in the present paper, caryophyllaeus brachycollis janiszewska, 1953 is redescribed and its two morphotypes are circumscribed on the basis of newly-collected specimens. morphotype 1 from barbels [barbus spp. including the type-h ...201424832186
artificial reproduction of wild and cultured barbel (barbus barbus, cyprinidae) under controlled conditions.the aim of this work was to compare the effects of controlled reproduction of cultured and wild common barbel, barbus barbus (l.). preparations containing different gnrh analogues and dopamine receptor antagonists (ovopel, ovaprim) as well as human chorionic gonadotropin (hcg) (in the case of cultured fish) were applied and their influence on ovulation, spermiation and quality of gametes obtained was determined. no differences in the qualitative or quantitative parameters of semen were found bet ...201121727068
heavy metal accumulation and the genotoxicity in barbel (barbus barbus) as indicators of the danube river pollution.the aim of this study was to analyze 16 trace elements (al, as, b, ba, cd, co, cr, cu, fe, li, mn, mo, ni, pb, sr, and zn) in different barbel (barbus barbus) tissues and to detect the presence of genotoxic effects in erythrocytes with the alkaline comet assay. barbel specimens were collected in the danube river near belgrade, serbia, where the discharge of untreated communal and industrial wastewaters is likely to produce negative effects on fish residing in this area. the highest concentration ...201222629137
trichopteran larvae (insecta) as the intermediate hosts of rhabdochona hellichi (nematoda: rhabdochonidae), a parasite of barbus barbus (pisces). 19957770436
myosin heavy chain isoforms in white, red and ventricle muscles of barbel (barbus barbus l.).1. actomyosin extracts of trunk, heart, and head muscles from barbel (barbus barbus l.) were analyzed by sds-polyacrylamide gel electrophoresis to study their myosin heavy chain composition. 2. four heavy chain isoforms were found: trunk white, trunk red, and ventricle muscles yielded one heavy chain typical of the muscle type; head muscles devoid of red fibers displayed two heavy chain isoforms, the slow migrating one corresponding to the trunk white muscle type. 3. the electrophoretic mobility ...19911799973
purification of pcb contaminated water by chitosan: a biological test of efficiency using the common barbel, barbus barbus. 19863098331
the microhabitat distribution of two dactylogyrus species parasitizing the gills of the barbel, barbus barbus.the microhabitat distribution of two congeneric species dactylogyrus carpathicus and d. malleus (monogenea) parasitizing the gills of the barbel (barbus barbus l.) was investigated. we tested whether congeneric species exhibited microhabitat preference and whether interspecific interactions could be attributed to the microhabitat segregation of congeners. the outlying mean index method was used to evaluate species microhabitats. gill variables (different microhabitats within gills) were used as ...200314627448
molecular characterization of the first non-mammalian p73 cdna.the p53 gene is believed to be mutated or deficient in over 50% of human tumours, and is therefore considered to be instrumental in the process of carcinogenesis. recently in humans, homologues of p53 (such as p73 and p63) have been isolated. in our studies in fish, we have been isolating tumour suppressor genes with a view to their potential use to study genotoxins in the aquatic environment. in this paper, we report the characterisation of the first non-mammalian p73 cdna, isolated from barbel ...200010825664
fatty acid composition of common barbel (barbus barbus) roe and evaluation of its haemolytic and cytotoxic activities.eggs of the common barbel (barbus barbus) cause intoxication in humans after ingestion. in this work, the chemical composition of the haemolytically active fraction from methanolic barbel roe extract was analyzed. compounds showing haemolytic activity and cytotoxicity towards normal and transformed cell lines were isolated and identified as polyunsaturated fatty acids, using online liquid chromatography-electrospray ionization mass spectrometry through tandem fragmentation experiments (hplc-ms/m ...201121510968
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