| molecular characterization of cryptosporidium from animal sources in qinghai province of china. | the presence of cryptosporidium oocysts in 20 zoo animals of the xining zoo, 16 farm yaks and 42 farm goats in qinghai province, china was investigated by an immunofluorescence test (ift). the species and/or genotypes were determined by nested polymerase chain reaction (pcr) and sequence analysis of a fragment of the small subunit (ssu) rrna gene. cryptosporidium oocysts were found in 16 zoo animals, 2 yaks, and 15 goats by ift. the ift positive samples were further investigated by pcr, and 16 o ... | 2007 | 17701218 |
| [roosting-site characteristics of wintering black-necked cranes (grus nigricollis) at napahai, yunnan]. | from november 2009 to april 2010, roosting-site characteristics of black-necked cranes (grus nigricollis) were observed at napahai provincial nature reserve, shangri-la, yunnan, china. the positions of roosting-sites were determined by triangulation with markers and field correction. all of the 63 roosting-sites observed were located in patchy marshes with water, which contained some mud on the bottom and 81% of the roosting-sites were covered by plants. they also had a certain distance to areas ... | 2011 | 21509960 |
| molecular and biochemical characterization of a novel multidomain xylanase from arthrobacter sp. gn16 isolated from the feces of grus nigricollis. | a novel glycosyl hydrolase family 10 (gh 10) xylanase (xynagn16), consisting of five domains, was revealed from the genome sequence of arthrobacter sp. gn16 isolated from the feces of grus nigricollis. xynagn16 and its truncated derivatives xynagn16l (gh 10 domain at n-terminus) and xynagn16lpd (gh 10 domain at n-terminus and polysaccharide deacetylases domain) were expressed in escherichia coli and characterized. biochemical characterizations and hydrolysis products analyses of recombinant xyna ... | 2015 | 25331377 |
| [a novel alpha-galactosidase from arthrobacter sp. gn14 isolated from grus nigricollis feces: gene cloning, heterologous expression and characterization]. | cloning and heterologously expressing the alpha-galactosidase gene (agaagn14) from arthrobacter sp. gn14 isolated from feces of black-neck crane (grus nigricollis). | 2012 | 22803347 |
| molecular and biochemical characterizations of a new low-temperature active mannanase. | a mannanase-coding gene was cloned from sphingobacterium sp. gn25 isolated from the feces of grus nigricollis. the gene encodes a 371-residue polypeptide (managn25) showing less than 74 % identity with a number of hypothetical proteins and putative glucanases and mannanases. before experiment's performance, managn25 was predicted to be a low-temperature active mannanase based on the molecular characterization, including (1) managn25 shared the highest identity of 41.1 % with the experimentally v ... | 2015 | 25868895 |
| kinetic and thermodynamic characterization of a novel low-temperature-active xylanase from arthrobacter sp. gn16 isolated from the feces of grus nigricollis. | we previously presented the cloning, heterologous expression, and characterization of a novel multidomain endoxylanase from arthrobacter sp. gn16 isolated from the feces of grus nigricollis. molecular and biochemical characterization studies indicate that the glycoside hydrolase (gh) family 10 domain at the n-terminus of the multidomain xylanase (rxynagn16l) is a low-temperature-active endoxylanase. many low-temperature-active enzymes contain regions of high local flexibility related to their ki ... | 2015 | 25587940 |
| why choose random forest to predict rare species distribution with few samples in large undersampled areas? three asian crane species models provide supporting evidence. | species distribution models (sdms) have become an essential tool in ecology, biogeography, evolution and, more recently, in conservation biology. how to generalize species distributions in large undersampled areas, especially with few samples, is a fundamental issue of sdms. in order to explore this issue, we used the best available presence records for the hooded crane (grus monacha, n = 33), white-naped crane (grus vipio, n = 40), and black-necked crane (grus nigricollis, n = 75) in china as t ... | 2017 | 28097060 |
| machine learning model analysis of breeding habitats for the black-necked crane in central asian uplands under anthropogenic pressures. | the black-necked crane (grus nigricollis) is the only alpine crane species and is endemic to the tibetan plateau. the breeding habitats of this species are poorly understood, which greatly hampers practical research and conservation work. using machine learning methods and the best-available data from our 7,000-kilometer mega-transect survey and open access data, we built the first species distribution model (sdm) to analyze the black-necked crane's breeding habitats. our model showed that curre ... | 2017 | 28733592 |
| nest-site selection, reproductive ecology and shifts within core-use areas of black-necked cranes at the northern limit of the tibetan plateau. | we investigated population dynamics, breeding pairs, breeding habitat selection, nest density, distance between neighboring nests, nest survival, reproductive success, and recruitment rate for black-necked cranes (bnc, grus nigricollis) during 2013-2015 in yanchiwan national nature reserve (ycw), gansu, china. numbers of bnc and breeding pairs remained relatively stable at around 140 individuals and 40 pairs. recruitment rates ranged from 15.7% to 25.8%. the average nest distance was 718.66 ± 43 ... | 2017 | 28168110 |
| winter diet and food selection of the black-necked crane grus nigricollis in dashanbao, yunnan, china. | the black-necked crane grus nigricollis is a globally vulnerable species whose food is the factor determining its long-term survival. understanding dietary habits, food preferences, and related factors will facilitate the development of effective conservation plans for the protection of this vulnerable species. for this purpose, we used video recordings and sampling of food availability to examine the dietary composition and temporal variation in food selection of black-necked cranes wintering i ... | 2016 | 27123382 |
| habitat preferences and conservation threats to black-necked cranes wintering in bhutan. | black-necked crane (grus nigricollis) is a vulnerable red list species whose populations are declining. however, little is known about black-necked cranes' habitat requirements or the causes of their population decline. we identified black-necked cranes' winter roost and foraging preferences of black-necked cranes in bhutan during the winter of 2013-2014. black-necked cranes' roosts were recorded using garmin gpsmap 60csx, while foraging preferences and threats to the birds were identified based ... | 2016 | 27026922 |
| habitat use, preference, and utilization distribution of two crane species (genus: grus) in huize national nature reserve, yunnan-guizhou plateau, china. | understanding the habitat use and spatial distribution of wildlife can help conservationists determine high-priority areas and enhance conservation efforts. we studied the wintering habitat use, preference, and utilization distribution of two crane species, that is, the black-necked crane (grus nigricollis, przevalski, 1876) and common crane (grus grus, linnaeus, 1758), in huize national natural reserve, yunnan-guizhou plateau, southwestern china. line transects indicated that anthropogenic farm ... | 2018 | 30042879 |
| home range and habitat use of breeding black-necked cranes. | to effectively protect a species, understanding its habitat needs and threats across its life-history stages is necessary. the black-necked crane (grus nigricollis) is an endangered wetland bird species of the qinghai-tibetan plateau, which is an important grazing area in china. to overcome the conflict between increasing grazing activities and the protection of wild cranes, we investigated the variation of habitat utilization within the home range of cranes at different stages (preincubation, i ... | 2020 | 33126424 |
| evaluation of potential ecological risk, possible sources and controlling factors of heavy metals in surface sediment of caohai wetland, china. | caohai, a plateau wetland in southwest china, is a national nature reserve providing protection for a variety of threatened and endangered species of migrant birds (e.g., the black-necked crane grus nigricollis). it has been experiencing the increasing environmental problems with heavy metals due to anthropogenic activities. however, the contamination of heavy metals in different habitats is unclear. surface sediment samples from these habitats were thus collected to analyze the distribution cha ... | 2020 | 32927581 |
| satellite tracking reveals a new migration route of black-necked cranes (grus nigricollis) in qinghai-tibet plateau. | the black-necked crane (grus nigricollis) is a vulnerable species and the only species that lives in the plateau. five migration routes of different populations have been identified, but for cranes wintering in nyingchi prefecture, tibet, the migration route and breeding/summering area are still unknown. the aim of this study was to investigate the spatio-temporal migration patterns of black-necked cranes in this area and to identify important areas for conservation. | 2020 | 32879798 |
| what factors influence the willingness of protected area communities to relocate? china's ecological relocation policy for dashanbao protected area. | resettlement of local people from protected areas (pas) has been regarded as a preferred method to alleviate human disturbance and environmental pressure. lack of knowledge about local communities' perceptions of resettlement, however, can lead to failed relocation projects and negative impacts on environmental sustainability and livelihoods. to better understand local communities' perception of relocation in pas, we examine factors that affect local communities' willingness to relocate in dasha ... | 2020 | 32334205 |
| characterization of the gut microbiome of black-necked cranes (grus nigricollis) in six wintering areas in china. | the black-necked crane (grus nigricollis) is a vulnerable species, breeding exclusively on the high-altitude wetlands of the qinghai-tibet plateau. bird species harbor diverse communities of microorganisms within their gastrointestinal tracts, which have important roles in the health, nutrition, and physiology of birds. hitherto, virtually nothing was known about the gut microbial communities associated with wild black-necked cranes. for the first time, this study characterized the gut microbial ... | 2020 | 31901964 |
| a high-quality draft genome assembly of the black-necked crane (grus nigricollis) based on nanopore sequencing. | the black-necked crane (grus nigricollis) which inhabits high-altitude areas has the largest body size of the world's 15 crane species, and is classified as threatened by the iucn. to support further studies on population genetics and genomics, we present a high-quality genome assembly based on both illumina and nanopore sequencing. in total, 54.59 gb illumina short reads and 116.5 gb nanopore long reads were generated. the 1.23 gb assembled genome has a high contig n50 of 17.89 mb, and has a lo ... | 2019 | 31725151 |
| dung-associated arthropods influence foraging ecology and habitat selection in black-necked cranes (grus nigricollis) on the qinghai-tibet plateau. | variation in grassland vegetation structure influences the habitat selection of insectivorous birds. this variation presents a trade-off for insectivorous predators: arthropod abundance increases with vegetation height and heterogeneity, but access to arthropod prey items decreases. in contrast, grazing by large herbivores reduces and homogenizes vegetation, decreasing total arthropod abundance and diversity. however, the presence of livestock dung may help counteract the overall reduction in in ... | 2019 | 30847095 |
| prevalence and genetic characterization of eimeriid coccidia from feces of black-necked cranes, grus nigricollis. | disseminated visceral coccidiosis (dvc) is a widely distributed intestinal and extraintestinal disease of cranes caused by eimeriid coccidia and has lethal pathogenicity to several crane species. here, feces of 164 black-necked cranes collected in dashanbao black-necked crane national nature reserve, china, were examined to determine the prevalence of coccidial oocysts. of the 164 fecal samples, 76 (46.3%) were positive for oocysts of eimeria, including e. gruis in 59 (35.9%), e. reichenowi in 5 ... | 2018 | 29383500 |
| can sequential vigilance be predicted? | sequential randomness is one of the three important assumptions for pulliam's vigilance model (1973). here we tested the sequential randomness in black-necked cranes grus nigricollis, to see if the vigilance sequence can be predicted. not similar to other recent studies, we found that most vigilance sequences (44/46) passed runs randomness test, and the length of an inter-scan interval was usually unrelated to its previous scan duration. our findings suggest high predation risk might favor a ran ... | 2017 | 29080805 |