| [the effect of trypsin inhibitor from vetch seeds on the growth of the rumen protozoan entodinium simplex in vitro]. |  | 1988 | 3144798 | 
| the metabolism of starch, glucose, amino acids, purines, pyrimidines and bacteria by the rumen ciliate entodinium simplex. |  | 1972 | 4625069 | 
| factors influencing agnotobiotic cultures of the rumen ciliate, entodinium simplex. | the nutrition of entodinium simplex was studied, with foliage of bluegrass (poa pratense), perennial ryegrass (lolium perenne), and grains of wheat (triticum vulgare) as substrates in agnotobiotic cultures. entodinium grew poorly when the substrates were autoclaved; better growth was obtained when the substrates were sterilized with ethylene oxide vapor. the concentration of ethylene oxide and the amount of moisture influenced the sterility and nutritional adequacy of the treated substrate. auto ... | 1968 | 4969666 | 
| the cultivation and some properties of the rumen ciliate entodinium simplex. |  | 1968 | 4971161 | 
| the cultivation of the rumen ciliate entodinium simplex. |  | 1969 | 4980724 | 
| rumen ciliates of tokara native goat in kagoshima, japan. | the composition of rumen ciliate protozoa was surveyed in 15 tokara native goats, capra hircus domesticus, inhibiting kagoshima, japan. as a result, of 11 species with 3 forms under 5 genera detected, entodinium simplex, e. nanellum and e. rectangulatum were found in all the animals examined. the large ophryoscolecid ciliates detected were polyplastron multivesiculatum and metadinium affine alone, which have been regarded as the typical members of type a composition proposed by eadie. since toka ... | 1995 | 7492663 | 
| phylogeny of the rumen ciliates entodinium, epidinium and polyplastron (litostomatea:entodiniomorphida) inferred from small subunit ribosomal rna sequences. | there complete 18s ribosomal rna gene sequences from the rumen ciliates, entodinium caudatum (1,639 bp), epidinium caudatum (1,638 bp), and polyplastron multivesiculatum (1,640 bp) were determined and confirmed in the opposite direction. trees produced using maximum parsimony and distance-matrix methods (least squares and neighbour-joining), with strong bootstrap support, depict the rumen ciliates as a monophyletic group. entodinium caudatum is the earliest branching rumen ciliate. however, ento ... | 1997 | 9172834 | 
| lipid metabolism of rumen ciliates and bacteria. i. uptake of fatty acids by isotricha prostoma and entodinium simplex. | washed suspensions of the ruminal ciliates, isotricha prostoma and entodinium simplex, concentrated c(14)-labeled oleic, palmitic, stearic, and linoleic acids within the cells during short incubation periods. radioautographs demonstrated that oleic acid-1-c(14) was hydrogenated to stearic acid by i. prostoma, and warburg manometric data showed that the sodium salts of oleic, valeric, caproic, and acetic acids, and methyl myristate, methyl laurate, and the triglyceride tributyrin stimulated ferme ... | 1962 | 13951437 | 
| lipid metabolism of rumen ciliates and bacteria. ii. uptake of fatty acids and lipid analysis of isotricha intestinalis and rumen bacteria with further information on entodinium simplex. | the total lipid and free fatty acid contents of isotricha intestinalis, entodinium simplex, and the rumen bacterial flora of the respective protozoa were determined. warburg manometric data showed that the sodium salts of tributyrin, oleic, and acetic acids stimulated gas production in i. intestinalis, whereas tributyrin was stimulatory with e. simplex and less active with oleic and acetic acids. rumen bacteria provided fatty acids produced lower manometric gaseous increases when compared with t ... | 1963 | 14000915 | 
| rumen bacterial and protozoal responses to insecticide substrates. | insecticides containing organophosphate, chlorinated hydrocarbon, and carbamate were tested with bovine ruminal ingesta fractions. rumen bacteria exposed to insecticide levels of 0 to 500 ppm in rumen fluid for 4 hr were inoculated into rumen fluid-starch feed extract medium. no apparent significant bacterial count inhibitions were noted. also, when insecticides were used as carbon sources at concentrations of 500 ppm in carbohydrate-limited media, no increases in bacterial counts were indicated ... | 1963 | 14075052 | 
| regeneration of cryoresistance of in vitro rumen ciliate cultures. | the purpose of this study was to investigate factors affecting mechanical- and cryo-resistance of the rumen ciliates entodinium caudatum (e.c.), entodinium furca monolobum (e.f.m.), entodinium simplex (e.s.), diplodinium denticulatum (two clones, d.d.01 and d.d.02), diploplastron affine (d.a.) and epidinium ecaudatum forma caudatum (e.e.c.) after long-term in vitro cultivation. following prolonged in vitro cultivation (more than six months), the ciliates were very sensitive to both centrifugatio ... | 2005 | 15950963 | 
| horizontal gene transfer from bacteria to rumen ciliates indicates adaptation to their anaerobic, carbohydrates-rich environment. | the horizontal transfer of expressed genes from bacteria into ciliates which live in close contact with each other in the rumen (the foregut of ruminants) was studied using ciliate expressed sequence tags (ests). more than 4000 ests were sequenced from representatives of the two major groups of rumen cilates: the order entodiniomorphida (entodinium simplex, entodinium caudatum, eudiplodinium maggii, metadinium medium, diploplastron affine, polyplastron multivesiculatum and epidinium ecaudatum) a ... | 2006 | 16472398 | 
| fluorescence in situ hybridization probing of protozoal entodinium spp. and their methanogenic colonizers in the rumen of cattle fed alfalfa hay or triticale straw. | to develop and test a fluorescence in situ hybridization (fish) based technique and to identify and quantify simultaneously those methanogenic populations colonizing entodinium spp. in the rumen of cows fed different forages. | 2014 | 24118832 | 
| design and validation of four new primers for next-generation sequencing to target the 18s rrna genes of gastrointestinal ciliate protozoa. | four new primers and one published primer were used to pcr amplify hypervariable regions within the protozoal 18s rrna gene to determine which primer pair provided the best identification and statistical analysis. pcr amplicons of 394 to 498 bases were generated from three primer sets, sequenced using roche 454 pyrosequencing with titanium, and analyzed using the blast database (ncbi) and mothur version 1.29. the protozoal diversity of rumen contents from moose in alaska was assessed. in the pre ... | 2014 | 24973070 |