| a review of recent immunochemical studies of blomia tropicalis and euroglyphus maynei allergens. | exposure to mites other than dermatophagoides spp., particularly blomia tropicalis and euroglyphus maynei, has been increasingly recognized as a cause of asthma. positive skin tests and serum ige antibodies to b. tropicalis have been reported in asthmatic patients from several areas of the world, including são paulo (brazil), hong kong and tampa (florida, usa). analysis of b. tropicalis extracts showed undetectable levels of the major group i and group ii dermatophagoides spp. allergens. immunoa ... | 1992 | 1493742 |
| prevalence of dust mites in the homes of people with asthma living in eight different geographic areas of the united states. | the density and species prevalence of dust mites were determined at various times over a 5-year-period in 252 homes of dust mite sensitive people with asthma who lived in eight geographic areas of the united states (cincinnati, ohio; new orleans, la.; memphis, tenn.; galveston, texas; greenville, n.c.; delray beach, fla.; san diego and los angeles, calif.). the most common dust mites found in the homes were dermatophagoides farinae (df), d. pteronyssinus (dp), euroglyphus maynei (em), and blomia ... | 1992 | 1527314 |
| sensitization to blomia tropicalis and lepidoglyphus destructor in dermatophagoides spp-allergic individuals. | serum was collected from 77 atopic subjects from cartagena, colombia, who had a positive skin test (greater than or equal to 3 mm) to either dermatophagoides farinae (df) (73) or d. pteronyssinus (dp) (70). twenty-nine healthy volunteers with negative allergy histories and negative skin tests were used as control subjects. rast and rast-inhibition assays were used to study the prevalence of specific ige to blomia tropicalis (bt), lepidoglyphus destructor (ld), and df. rast results were considere ... | 1991 | 1744365 |
| exposure and sensitization to dust mite allergens among asthmatic children in são paulo, brazil. | a group of 20 mite allergic asthmatic children aged 6-12 years old, living in são paulo, brazil, was studied regarding their degree of sensitization to house dust mites and exposure to mite allergens in their homes. in 18 out of 20 houses at least one dust sample was obtained which contained greater than 10 micrograms der p i/g of dust. the highest levels of dermatophagoides pteronyssinus allergens, der p i and group ii, were measured in bedding samples (geometric mean 38.4 and 36.6 micrograms/g ... | 1991 | 1913266 |
| house dust mite allergy in florida. mite survey in households of mite-sensitive individuals in tampa, florida. | this study evaluated the prevalence of positive house dust mite skin tests in a population of atopic individuals and identified the mite species present in mattress and house dust samples in homes of the tampa bay area. four hundred consecutive individuals were evaluated for respiratory complaints and skin tested with standardized extracts of dermatophagoides pteronyssinus (dp) and dermatophagoides farinae (df). two hundred forty individuals (60%) had a positive skin test to the mite extracts. d ... | 1990 | 2292372 |
| house dust mites in caracas, venezuela. | an investigation of bedding dust fauna was carried out in caracas, venezuela. mites were recovered from all samples. the average mite density of 15.6 mites/mg dust was among the highest reported worldwide. pyroglyphid mites were the most frequent and abundant, and dermatophagoides pteronyssinus was the largest contributor to the total mite counts. it was also found that the storage mite blomia tropicalis occurred in 96% of the samples examined and contributed an average of 15% of the total mites ... | 1987 | 3619131 |
| [skin tests for different species of acari in asthmatic children from mexico city]. | house dust mites, dermatophagoides pteronyssinus (dp) and dermatophagoides farinae (df) are the predominant cause of allergic asthma in our population. to detect the presence of sensitivity to other mites besides dp and df in 100 children that live in mexico city, skin tests were performed in their arms with multi-test devices and glycerinated extracts. there were 34 female and 66 male, aged 4 to 14 years (x = 9.1y), with history of mild and moderate asthma, 2 months to 12 years of evolution. dp ... | 1995 | 7552183 |
| [prevalence of sensitization to blomia in gran canaria]. | in the present study we assessed the prevalence of sensitizations to blomia in our environment. perennial climatological conditions in the canarian islands facilitates the habitat to mites. the blomia is found in tropical and subtropical homes of europe, usa and asia like the dermatophagoides. we have designed a transversal and prospective study on 100 patients who consulted our unit for the first time. a prick-test for the common allergens, including blomia kulagini, and in vitro determination ... | 1995 | 7572538 |
| a naturally occurring model of immunoglobulin e antibody-mediated hypersensitivity in laboratory animals. | a naturally occurring model of immunoglobulin e (ige) antibody-mediated hypersensitivity in canine and rhesus monkey species to environmental allergens is described. cutaneous responses to a dust mite mix (dermatophagoides farinae/pteronyssinus) and to four storage mite extracts (aleuroglyphus ovatus, blomia tropicalis, chortoglyphus sp., lepidoglyphus destructor) occurred in the canine species. rhesus monkeys had ige antibody-mediated hypersensitivity to dust mite on cutaneous challenge, but to ... | 1994 | 8201269 |
| mite allergy in the tropics: sensitization to six domestic mite species in cartagena, colombia. | sensitization to the domestic mites blomia tropicalis, chortoglyphus arcuatus, lepidoglyphus destructor and aleuroglyphus ovatus was investigated in 77 subjects with clinical symptoms of asthma and/or allergic rhinitis and a positive skin prick test (wheal > or = 3 mm) to dermatophagoides pteronyssinus and/or d. farinae. seventy patients were skin test-positive to d. pteronyssinus and 73 to d. farinae. serum samples from 29 nonallergic, skin test-negative individuals were used as negative contro ... | 1993 | 8281353 |
| mite fauna, der p i, der f i and blomia tropicalis allergen levels in a tropical environment. | fifty dust samples were collected from the mattresses and bedroom floors of 25 subjects with allergic asthma in cartagena, colombia, in order to identify house dust mites and quantitate der p i, der f i and blomia tropicalis allergens. the geometric mean of the total mite density per gram of dust was 418 (range, 40-2280). twenty-two samples (44%) had more than 500 mites and four, less than 100. b. tropicalis and dermatophagoides pteronyssinus were found in 96% and 90% of the samples, accounting ... | 1993 | 8319126 |
| igg and ige antibody responses to dust mite allergens among children with asthma in brazil. | we compared exposure and levels of ige and igg antibodies to inhalant allergens in 20 children with asthma and positive skin tests to dermatophagoides pteronyssinus, living in são paulo, to 20 control children without history of asthma or allergy and negative skin tests to d. pteronyssinus, d. farinae, and blomia tropicalis. in 16/20 and 17/20 houses of asthmatic and control children, respectively, at least one sample was obtained which contained > 10 micrograms der p i/g of dust. serum ige anti ... | 1993 | 8346869 |
| allergenicity of the mite, blomia tropicalis. | blomia tropicalis (bt) occurs in a significant percentage of homes in tropical and subtropical geographic regions of the united states and europe and in countries in south america and asia along with the pyroglyphid mites, euroglyphus maynei (em), dermatophagoides pteronyssinus, and d. farinae. blomia species may be major sources of allergens in house dust in addition to dermatophagoides species and e. maynei. | 1993 | 8491936 |
| characterization of allergenic components from house dust mite dermatophagoides siboney. purification of der s 1 and der s 2 allergens. | allergic reactions to house dust mites of the genus dermatophagoides play an important role in the pathogenesis of asthma and other atopic diseases. dermatophagoides siboney has been described as a species from cuba. together with d. pteronyssinus and blomia tropicalis, it is frequently found in house dust from homes of asthmatics. | 1995 | 8556562 |
| crossreactivity between dermatophagoides siboney and other house dust mite allergens in sensitized asthmatic patients. | the role of pyroglyphid mites in ige-mediated sensitization has been well established. although mites belonging to the genus dermatophagoides dominate in the acarofauna of domestic dust, non-pyroglyphid mites might also be of clinical importance. in cuba, dermatophagoides siboney is found in dust coexisting with d. pteronyssinus and blomia tropicalis. dermatophagoides farinae is not found. storage mites, such as acarus siro, lepidoglyphus destructor and tyrophagus putrescentiae, might also be pr ... | 1995 | 8556563 |
| allergen-specific ige levels and mite allergen exposure in children with acute asthma first seen in an emergency department and in nonasthmatic control subjects. | sensitization to allergens has been shown to be a risk factor for adults with acute asthma first seen in the emergency department. | 1996 | 8757201 |
| sensitization to dermatophagoides siboney, blomia tropicalis, and other domestic mites in asthmatic patients. | mite species adapted to warm, humid climates are commonly found in house dust in the tropics. in cuba, dermatophagoides pteronyssinus, d. siboney, and blomia tropicalis are the most common and abundant mite species in house dust. to investigate the pattern of sensitization of cuban asthmatic patients to common mite species, we skin-prick-tested (spt) 148 patients with a clinical history of asthma and possible mite allergy, and determined specific ige antibodies against mite allergens (d. pterony ... | 1996 | 8863927 |
| cross-allergenicity of the house dust mites euroglyphus maynei and blomia tropicalis. | the domestic mites euroglyphus maynei and blomia tropicalis frequently co-inhabit homes in subtropical/tropical regions around the world. both species are the source of substances that cause allergic reactions in patients. | 1996 | 8933777 |
| nucleotide sequence analysis of a complementary dna coding for a blomia tropicalis allergen. | blomia tropicalis is a mite of allergenic importance in tropical and subtropical areas. a clone (bt11a) from a b. tropicalis complementary dna library was expressed in lambda phage and analyzed by plaque radioimmunoassay. the recombinant allergen produced by this clone was bound by ige in 16 of 32 sera from individuals with asthma with a positive rast response and none of 3 control sera from healthy individuals with negative rast response to b. tropicalis. the cdna insert was amplified by polyme ... | 1996 | 8939156 |
| mite sensitivity and exposure in the city of santa fe, argentina. | little information is available on mite allergen levels and rates of sensitization to domestic mites in south america. the purpose of this study was to establish the rate of sensitization to domestic mites in asthmatics and measure mite allergen levels in pillows and mattresses of mite allergic individuals in santa fe, argentina. dust samples were collected from the pillows and mattresses of 56 asthmatics (24 females and 32 males, mean age 17.6 +/- 11.5 years), who had been previously skin teste ... | 1996 | 8959537 |
| sensitization to blomia tropicalis in patients with asthma and identification of allergen blo t 5. | in tropical and subtropical regions of the world, allergens produced by blomia tropicalis are an important cause of ige-mediated sensitization among patients with asthma. we compared the relative importance of sensitization to the two mite species among asthma patients from florida, puerto rico, and brazil (n = 83), who were concurrently exposed to b. tropicalis and d. pteronyssinus, with patients from the united states and from the united kingdom (n = 56) exposed to d. pteronyssinus. in additio ... | 1997 | 9001334 |
| hla-dr3 is associated with the ige immune responsiveness to a recombinant allergen from blomia tropicalis (bt). | | 1996 | 9095226 |
| cloning and ige binding of a recombinant allergen from the mite blomia tropicalis, homologous with fatty acid-binding proteins. | to characterize the allergens of blomia tropicalis, a cdna library was constructed and screened with allergic sera from asthmatic patients. one clone, bt6, was subcloned and sequenced. the nucleotide sequence of 934 bp length shows a 390-bp reading frame which encodes a 130-amino acid protein with a mw 14.8 kd. no potential glycosylation site was found in the predicted protein. the inferred amino acid sequence has no homology to known allergens. it has a cytosolic fatty acid-binding protein (fab ... | 1997 | 9104789 |
| identification of the domestic mite fauna of puerto rico. | this study was conducted to identify the domestic mite fauna of puerto rico. a total of 57 dust samples were collected from mattresses in homes of 11 cities on the island. the analysis of the samples revealed that 73.70% of the mattress samples had at least one mite species. the identified species include: dermatophagoides pteronyssinus (45.6%), blomia tropicalis (31.6%), cheyletus sp. (19.3%), dermatophagoides farinae (17.5%), euroglyphus maynei (5.3%), dermatophagoides sibonei (1.8%), dermatop ... | 1997 | 9285987 |
| prevalence and distribution of indoor allergens in singapore. | immediate hypersensitivity to indoor allergens is known to be associated with allergic asthma. this study evaluated the prevalence and distribution of six indoor allergens in 956 dust samples obtained from homes, childcare centres, schools, and a hospital in tropical singapore. seasonality of mite allergens was also assessed. | 1997 | 9291283 |
| ige antibodies to aeroallergens in allergic children in são paulo, brazil. | the sensitization to inhaled allergens was studied in 80 brazilian children and 12 controls, aged 6 to 16 years. we performed skin tests with alternaria alternata, cat, dog, lolium perenne, grasses and the following domestic mites: blomia tropicalis, dermatophagoides pteronyssinus, dermatophagoides farinae, lepidoglyphus destructor, chortoglyphus arcutus and aleuroglyphus ovatus. the frequencies of positive skin tests (mean diameter > or = 23 mm) were respectively 15%, 11%, 11%, 6%, 7%, 95%, 92% ... | 1997 | 9330189 |
| allergenicity of blomia tropicalis. | | 1997 | 9416560 |
| sensitization to blomia tropicalis among asthmatic patients in taiwan. | house dust mites have been reported as one of the most important allergens in taiwan especially in asthmatic patients. this study was conducted to determine the allergenicity of blomia tropicalis and sensitization of asthmatic patients in taiwan. serial dust samples were collected every month between july 1993 and june 1994 from 13 houses of mite-allergic patients. about 1 m2 surface area of a quilt was vacuumed. the floating method was used to collect mites, then identification and counting wer ... | 1998 | 9482703 |
| prevalence of skin reactions to aeroallergens in asthmatics of puerto rico. | in puerto rico, although a high prevalence of asthma has been reported, the sensitization rates to aeroallergens in these patients is unknown. the purpose of this study using a case control design, was to determine and compare the rates of sensitization to common aeroallergens in an asthmatic population of 576 asthmatics and 144 healthy controls. a skin prick test was conducted using standardized extracts of dermatophagoides farinae (df) and d. pteronyssinus (dp), house dust (hd), cat hair and e ... | 1997 | 9580406 |
| sensitization to mite allergens and acute asthma in a tropical environment. | the objective of this study was to determine whether sensitization to certain aeroallergens is associated with acute asthma in cartagena, colombia. ninety-nine subjects with acute asthma and 100 controls evaluated for other reasons were studied at a local emergency room. sensitization was established by specific ige determinations. statistically significant differences between asthmatic subjects and controls were found in the prevalence of specific ige to dermatophagoides pteronyssinus, 64.6% vs ... | 1998 | 9827423 |
| house dust mite fauna of tropical singapore. | sensitization to house dust mites is very common in the tropics. this study evaluated the dust mite fauna in singaporean homes. | 1999 | 10051724 |
| biologic activity of dermatophagoides siboney and blomia tropicalis allergens in exposed and unexposed mite-allergic individuals. effect of patient selection on the biologic standardization of mite extracts. | this study aimed to investigate the influence of patient selection criteria, i.e., mite-allergic individuals exposed and not exposed to dermatophagoides siboney and blomia tropicalis, on the biologic activity of mite extracts. determination of the potency of mite extracts in vivo requires selection of patients with a clinical history of mite allergy. in scandinavia, there are some anamnestic criteria for mite allergy, whereas in the tropics, where patients are continuously exposed to high levels ... | 1999 | 10371100 |
| allergenic differences between the domestic mites blomia tropicalis and dermatophagoides pteronyssinus. | house dust mite allergens are the most important indoor allergens associated with asthma and rhinitis in singapore and the tropics. recent data to suggest that besides the dermatophagoides spp., the domestic mite blomia tropicalis (bt) is also an important source of allergens in these regions. | 1999 | 10383600 |
| prevalence of allergic sensitization to regional inhalants among allergic patients in jakarta, indonesia. | sensitization towards a panel of eight regional inhalant allergens was evaluated among 107 patients with allergic rhinitis and/or asthma. a total of 32 children (age 5-13 years, mean 9 years; 18 male, 14 female), 75 adolescents and adults (aged 14-66 years, mean 32 years; 21 male, 54 female) and 20 normal control volunteers (aged 16-46, mean 30 years; 4 male, 16 female) were evaluated via skin prick test. a weal response of 3 x 3 mm or greater was taken to be positive. the sensitization rates am ... | 1999 | 10403003 |
| identification and characterisation of two allergens from the dust mite acarus siro, homologous with fatty acid-binding proteins. | dust mites are a major cause of allergic disease worldwide. the dust mite acarus siro is an inducer of occupational allergy among farmers, but sensitisation has also been found in non-farming populations. | 1999 | 10474032 |
| sensitization to local dust-mite fauna in singapore. | recent studies showed the presence of a unique dust-mite fauna in the indoor environment of singapore. immediate hypersensitivity to these dust mites, along with other known indoor allergens, may play a role in the pathogenesis of allergic respiratory diseases. this study evaluated the sensitization rates of the local atopic population to these allergens. | 1999 | 10604550 |
| sensitization to blomia tropicalis and dermatophagoides pteronyssinus-a comparative study between singapore and taiwan. | blomia tropicalis (bt) and dermatophagoides pteronyssinus (dp) are the predominant domestic mites species in singapore and taiwan. this study aims to characterize and compare the mite sensitization profiles in both countries. skin prick tests were performed on 203 singaporeans with dp and bt crude extracts. in vitro ige and igg4 reactivity to extracts and specific allergens (der p 1, der p 2 der p 5 and blo t 5) were determined by immunoassays. approximately 91% of the tested singaporeans were s ... | 1999 | 10697257 |
| blomia tropicalis sensitization as a risk factor for the development of allergic rhinitis. | | 2000 | 10752925 |
| dermatophagoides farinae (der f 1) and dermatophagoides pteronyssinus (der p 1) allergen exposure among subjects living in uberlândia, brazil. | the role of mite allergen exposure in sensitization and development of asthma has been widely recognized. previous studies have shown that dermatophagoides pteronyssinus and blomia tropicalis were the most prevalent house dust mites in brazil, while d. farinae was rarely found. the aim of this study was to measure der f 1 and der p 1 allergen levels in brazilian asthmatics' and controls' homes. | 2000 | 10971116 |
| [diagnostic clinical trial in children using an allergenic extract of blomia tropicalis]. | blomia tropicalis is a mite that is one of the most important causes of allergic sensitization in studies of tropical and subtropical regions. nonetheless, there are no published studies of the sensitivity and specificity of b. tropicalis extracts. | 2000 | 11022269 |
| house dust mites in the city of lima, peru. | since mites are the most common house dust allergens, knowledge about the species most prevalent in a region is important for diagnostic and specific immunotherapy purposes. in order to establish the prevalence of house dust mites in different city districts, 100 house dust samples were collected from different parts of lima. lima is a city of tropical climate located along the coast of the pacific ocean. the relative air humidity is 80-90% and the various districts studied are located at altitu ... | 2000 | 11108440 |
| sensitization to different mite species in german farmers: clinical aspects. | various mite species referred to collectively as house dust and storage mites are recognized worldwide as a cause of allergic airway disease. our study aimed to investigate the frequency of sensitization and potential importance of mite species in farmers using a broad mite spectrum. a total of 86 german farmers with rhinitis and/or asthma were studied by skin prick testing and/or enzyme allergosorbent test (east) with the following mites: blomia tjibodas, blomia tropicalis, blomia kulagini, gly ... | 2000 | 11206935 |
| evaluation of the acarofauna of the domiciliary ecosystem in juiz de fora, state of minas gerais, brazil. | from august 1999 to january 2000, samples of house dust were collected from 160 domiciles in the city of juiz de fora, state of minas gerais, brazil. in 36 of these domiciles kitchen samples were obtained. prevalence rate was 77.5%, varying according to the geographical sector. there were found 2,278 specimens of mites, with 1,530 (67.2%) in the adult stage and 748 (32.8%) in immature forms. the main species found were dermatophagoides pteronyssinus, d. farinae, euroglyphus maynei, blomia tropic ... | 2001 | 11685254 |
| cdna cloning and expression of blo t 11, the blomia tropicalis allergen homologous to paramyosin. | blomia tropicalis is an important mite species in many parts of the world and the most predominant mite species in tropical countries. the prevalence of sensitization to this species has probably been underestimated because commercial extracts are largely unavailable. identification and characterization of b. tropicalis allergens is an important step toward understanding the role of this species in allergic sensitization and could provide appropriate reagents for diagnostic and therapeutic proce ... | 2001 | 11815735 |
| the clinical efficacy of in vitro allergen-specific ige antibody test in the diagnosis of allergic children with asthma. | asthma is a very common respiratory allergic disease in taiwan. the aims of this study were to investigate the allergen-sensitized profile and its relationship with serum total ige levels in allergic asthmatic children in taiwan. moreover, the number of allergens to be tested for the most efficient and effective diagnosis of allergic diseases was also examined. total ige and ige specific for a panel of common aeroallergens were assayed in 200 serum samples of asthmatic children using pharmacia c ... | 2002 | 11890224 |
| pattern of sensitization to common environmental allergens amongst atopic singapore children in the first 3 years of life. | this study was aimed to investigate the sensitization pattern to a range of common allergens in young singaporean children. a cross-sectional study involving 75 children aged below 3 years was carried out. they presented between december 1995 and april 2000 with symptoms of asthma, rhinitis, eczema, or food allergy. their levels of allergen-specific serum ige to a panel of foods (egg white, milk, soy protein, shrimp, wheat and peanut), pet dander, dust mites and cockroaches were measured with ph ... | 2001 | 12009071 |
| reactivity of anti-blomia tropicalis igg and ige in patients with atopic dermatitis. | patients with atopic dermatitis (ad) commonly develop antibodies of subclasses igg and ige to house dust mites, such as dermatophagoides pteronyssinus and d. farinae. the domestic mite blomia tropicalis is prevalent in brazil and can cause the exacerbation of ad. the objectives of this study were to assess skin reactivity to b. tropicalis extracts of ad patients and a control group of nonallergic subjects, and to determine the in vitro reactivity of anti-b. tropicalis igg and ige in the serum of ... | 2002 | 12139678 |
| dust mite allergens: ecology and distribution. | the most important allergy-causing mites found in homes worldwide are the house dust mites dermatophagoides farinae, d. pteronyssinus, euroglyphus maynei, and the storage mite blomia tropicalis. most homes contain multiple species. the most prevalent mite species and allergen in homes differ geographically, between homes within a geographical region, and among areas within a home. therefore, it is important to know which mite species are present in a geographical area when performing diagnostic ... | 2002 | 12165207 |
| identification of shared and unique immunoglobulin e epitopes of the highly conserved tropomyosins in blomia tropicalis and dermatophagoides pteronyssinus. | tropomyosin belongs to a class of highly conserved proteins in invertebrates and vertebrates. the invertebrate tropomyosins are allergenic in man with high ige cross-reactivity and have been therefore referred to as pan-allergens. | 2002 | 12190660 |
| monoclonal antibodies against blo t 13, a recombinant allergen from blomia tropicalis. | the recombinant allergen of blomia tropicalis, rblo t 13, shows 11% of ige reactivity to sera from allergic patients. this allergen belongs to the fatty acid-binding protein family and its natural equivalent remains to be isolated. monoclonal antibodies (mabs) are important tools for specific determination and isolation of natural allergens as well as for characterization of recombinant proteins. | 2002 | 12444318 |
| association between mite allergen (der p 1, der f 1, blo t 5) levels and microscopic identification of mites or skin prick test results in asthmatic subjects. | mite allergens have been involved in airway sensitization and allergic diseases. immunoassays for the identification and quantifiction of house dust mite (hdm) allergens are useful to improve the knowledge of regional mite fauna and the remediation of mite allergens in allergic diseases. the present study analyzed the association between levels of hdm allergen and results of mite identification or skin prick test (spt) in two different areas of bahia, brazil. | 2002 | 12444321 |
| serum immunoglobulin e against storage mite allergens in dogs with atopic dermatitis. | to determine the prevalence of serum ige against the storage mites acarus siro, blomia tropicalis, and tyrophagus putrescentiae in a population of dogs with atopic dermatitis. | 2003 | 12518875 |
| in vivo and in vitro sensitization to domestic mites in german urban and rural allergic patients. | sensitization to domestic mites is common in germany. the main objectives of this study were (1) to establish the rate of skin test sensitivity to dermatophagoides pteronyssinus, acarus siro, lepidoglyphus destructor, and tyrophagus putrescentiae in 512 consecutive patients evaluated for upper and/or lower respiratory complaints; (2) to verify how many of the patients with a positive skin test to at least one of the three storage mites were also skin test positive to d. pteronyssinus; and (3) to ... | 2002 | 12530116 |
| cloning and expression of blo t 1, a novel allergen from the dust mite blomia tropicalis, homologous to cysteine proteases. | house dust mite allergens have been shown to be a very important stimulus in the causation of asthma and triggers for the exacerbation of symptoms. therefore, characterization of mite-derived allergens at the molecular level is an important step for the development of effective diagnostic and therapeutic approaches, as well as for epidemiological studies. | 2003 | 12534546 |
| oral tolerance induction to dermatophagoides pteronyssinus and blomia tropicalis in sensitized mice: occurrence of natural autoantibodies to immunoglobulin e. | the dust mites dermatophagoides pteronyssinus (dp) and blomia tropicalis (bt) are important sources of indoor allergens in tropical and subtropical countries. murine models allow the analysis of the immune response and regulation of ige production to dp and bt allergens. oral tolerance induces unresponsiveness in naive animals, but its application in sensitized animals can provide useful information to improve allergy therapy. | 2002 | 12569990 |
| skin test reactivity to natural and recombinant blomia and dermatophagoides spp. allergens among mite allergic patients in the uk. | many asthmatics in tropical and subtropical areas have positive skin prick tests to both dermatophagoides spp. and to the mite blomia tropicalis. this may be due to recognition by ige of cross-reactive allergens between the different mite species or because of sensitization to species-specific allergens. a 14-kda blomia tropicalis allergen, blo t 5, has been cloned and shows 40% sequence homology with der p 5. the aim of this study was to investigate reactivity to b. tropicalis in patients known ... | 2003 | 12580807 |
| the upper and lower airway responses to nasal challenge with house-dust mite blomia tropicalis. | the house dust mite blomia tropicalis (b. tropicalis) was found to be the most prevalent domestic mite in singapore. however, its pathogenicity in allergic airway diseases remains to be investigated. | 2003 | 12580812 |
| specific ige to 5 different major house dust mites among asthmatic children. | asthma is one of the most commonly occurring manifestation of allergy in taiwan. sensitivity to house dust mites is closely related to childhood asthma. this study was designed to investigate sensitized rates and average concentrations of specific ige antibodies to 5 major house dust mites (hdms) among asthmatic children. a total of 93 asthmatic children aged from 3 to 15 years were enrolled to measure their specific ige concentrations in response to 5 different species of mites: dermatophagoide ... | 2002 | 12607482 |
| an extensive study of human ige cross-reactivity of blo t 5 and der p 5. | dual sensitization by blomia tropicalis and dermatophagoides pteronyssinus mites is common in tropical and subtropical countries. the human ige cross-reactivity between clinical important group 5 allergens, blo t 5 and der p 5, remains controversial. | 2003 | 12642844 |
| peptide mapping of immunoglobulin e and immunoglobulin g immunodominant epitopes of an allergenic blomia tropicalis paramyosin, blo t 11. | the identification of immunodominant peptides containing the ige and igg epitopes on allergen molecules is an important step in understanding the interaction of the allergen with the immune system and, thus, essential for the development of effective immunotherapeutic and diagnostic reagents. the present study aimed to map the ige and igg immunodominant peptides of blomia tropicalis (bt) allergen blo t 11, a high molecular weight allergen homologous to paramyosin, exhibiting important allergenic ... | 2003 | 12680869 |
| ecological analysis of acari recovered from coprolites from archaeological site of northeast brazil. | coprolite samples of human and animal origin from the excavations performed at the archaeological site of furna do estrago, at brejo da madre de deus in the state of pernambuco, brazil and sent to the paleoparasitology laboratory at escola nacional de saúde pública-fiocruz, rio de janeiro, were analyzed for mites. after rehydratation and sedimentation of the coprolites, the alimentary contents and the sediments were examined and the mites collected and prepared in definitive whole mounts, using ... | 2003 | 12687780 |
| cloning of a group 3 allergen from blomia tropicalis mites. | blomia tropicalis is an important mite species in the tropics and subtropical regions of the world. it is well established that the allergen from this species of mite is one of the triggering factors for allergic asthma. the isolation and characterization of allergens in this mite species is desired to provide sensitive and specific reagents for diagnostic as well as therapeutic purposes. | 2003 | 12708986 |
| sensitization to dermatophagoides pteronyssinus and blomia tropicalis extracts and recombinant mite allergens in atopic thai patients. | mite surveys in thailand indicated that dermatophagoides pteronyssinus (dp) is predominant, but so far there were no data available on blomia tropicalis (bt), which is prevalent in the asia pacific region. skin prick testing (spt) was performed in 40 atopic children, 45 atopic adults and 17 non-atopic volunteers. skin reactions to dp were found in 25/40 (62.5%) and 23/45 (51.1%); skin reactions to bt were found in 15/40 (37.5%) and 18/45 (40%) in atopic children and adults, respectively. spt to ... | 2002 | 12744621 |
| comparative allergenicity studies of native and recombinant blomia tropicalis paramyosin (blo t 11). | the complementary dna (cdna) encoding for blo t 11, a 102 kd allergen from blomia tropicalis (bt) was isolated, expressed and characterized previously. this study aimed to isolate the native blo t 11 allergen and compare its allergenicity with the recombinant forms. | 2003 | 12752328 |
| generation of monoclonal antibodies against blo t 3 using dna immunization with in vivo electroporation. | house dust mite allergy is closely associated with allergic diseases. blomia tropicalis mite species is an important clinical species in the tropics. the cdna clone encoding blo t 3, a group 3 allergen from b. tropicalis, has been isolated in our laboratory. | 2003 | 12752596 |
| mite and cockroach sensitization in allergic patients from caracas, venezuela. | mites and cockroaches are important allergen sources in most countries, especially in the tropics. they have been incriminated as the cause of the increased prevalence of inhalant allergen sensitization that has occurred in many areas of latin america. | 2003 | 12839327 |
| skin tests and conjunctival and bronchial challenges with extracts of blomia tropicalis and dermatophagoides pteronyssinus in patients with allergic asthma and/or rhinoconjunctivitis. | blomia tropicalis and dermatophagoides pteronyssinus are important mite species in tenerife, canary islands, spain. several studies have demonstrated a variable degree of allergenic cross-reactivity in vitro. however, only a few have addressed their allergenic cross-reactivity using challenge tests. | 2003 | 12876408 |
| lack of human ige cross-reactivity between mite allergens blo t 1 and der p 1. | the group 1 mite allergens are the most significant indoor allergens and they belong to the papain-like cysteine protease family. to date there is only one published report on the isolation and characterization of group 1 allergens from blomia tropicalis mites. the aims of the study are to determine the cross-reactivity between group 1 allergens and to evaluate their clinical importance in allergic patients. | 2003 | 12911421 |
| sensitization profiles of malaysian and singaporean subjects to allergens from dermatophagoides pteronyssinus and blomia tropicalis. | the house dust mites dermatophagoides pteronyssinus (der p) and blomia tropicalis (blo t) are the most common house dust mite species in southeast asia. to date, there have only been a few studies on the sensitization profile of the general populations in southeast asia to house dust mites. the aim of this study was to determine the profiles of der p and blo t sensitization among singaporean and malaysian subjects. | 2003 | 14646382 |
| [positive skin test and age] | objective: to evaluate positive responses to skin tests for immediate hypersensitivity to allergens in children with asthma and rhinitis at different ages. method: we observed positive skin test reactivity in prick tests using fifteen allergens of same origin (total dust and dermatophagoides sp.; dermatophagoides pteronyssinus; dermatophagoides farinae; blomia tropicalis; penicillium sp; alternaria alternata; cladosporium herbarium; aspergillus fumigatus; bermuda grass; forage grass; dog and cat ... | 2001 | 14647601 |
| [risk factors, clinical and laboratory aspects of asthma in children] | objective: to evaluate the clinical and laboratory aspects and the risk factors associated with asthma in children treated at the pediatric outpatient clinic of hospital universitário júlio müller. methods: a case-control study including 59 asthmatic children (cases) and 104 nonasthmatic children (controls). the following factors were considered for risk analysis: parentś level of education, domestic exposure to allergens, passive smoking, breast feeding, low income, and family history of allerg ... | 2001 | 14647823 |
| identification of the major allergenic components in blomia tropicalis and the relevance of the specific ige in asthmatic patients. | blomia tropicalis has been reported to be a clinically important allergen in house dust. high prevalence of sensitization to b. tropicalis has been noted in asthmatic patients in taiwan; however, the allergenic components and its impact on asthmatic patients remain to be clarified. | 2003 | 14692433 |
| ige reactivity from serum of blomia tropicalis allergic patients to the recombinant protein blo t 1. | to determine the ige reactivity against recombinant protein blo t 1 from the dust mite blomia tropicalis (bt) using serum from patients with positive skin test to this mite and to investigate the cross-reactivity between bt and dermatophagoides pteronyssinus. | 2003 | 14768500 |
| high-level expression of a codon optimized recombinant dust mite allergen, blo t 5, in chinese hamster ovary cells. | blo t 5 is a major allergen from house dust mite blomia tropicalis. purification of native blo t 5 (nblo t 5) from whole dust mite extract is tedious and gave low yield. in this study, we demonstrated that codon optimization facilitated high-level expression of blo t 5 in chinese hamster ovary (cho)-k1 cells and thus allows production of sufficient recombinant cblo t 5 for specific immunotherapy. a codon optimized blo t 5 gene was synthesized by pcr and the codon optimized or wild-type blo t 5 g ... | 2004 | 15044082 |
| allergy to house dust mites and asthma. | house dust mites have been shown to be important sources of indoor allergens associated with asthma and other allergic conditions. asthma is a chronic respiratory disease that affects millions of people worldwide, and numerous scientific studies have shown that the prevalence of asthma is increasing. the most common dust mite species around the world include dermatophagoides pteronyssinus (dp), dermatophagoides farinae (df), euroglyphus maynei (em) and blomia tropicalis (bt). over the past three ... | 2004 | 15125219 |
| importance of including blomia tropicalis in the routine diagnosis of venezuelan patients with persistent allergic symptoms. | blomia tropicalis is a common mite found in the house dust of many tropical countries including venezuela. the prevalence of skin test and specific serum immunoglobulin (ig)e reactivity to b. tropicalis in venezuela has not been previously evaluated. | 2004 | 15180763 |
| immunoglobulin e reactivity of native blo t 5, a major allergen of blomia tropicalis. | blo t 5 is a major allergen of blomia tropicalis and its complementary dna (cdna) has been expressed in both prokaryotic and eukaryotic expression systems. although the recombinant blo t 5 has been well characterized, relatively less is known about its native counterparts and the allergenicity comparison of the native and recombinant blo t 5 allergens has not been reported. | 2004 | 15544602 |
| mite allergen levels and acarologic analysis in house dust samples in uberaba, brazil. | mite allergen exposure has been widely related to sensitization and development of allergic diseases. this study intended to evaluate the degree of allergen exposure in uberaba, brazil, through the measurements of der f 1 and der p 1 allergen levels associated with the acarologic analysis in house dust samples. a total of 240 dust samples were collected from 60 houses through vacuuming sofas and bedding, during the months of march and july 2000. indoor temperature and relative humidity were also ... | 2004 | 15552718 |
| allergenic composition of the mite suidasia medanensis and cross-reactivity with blomia tropicalis. | mites of the genus suidasia are commonly found in house dust and may play an allergenic role in exposed populations. however, the allergenic potential and clinical impact of this genus has not been well established. the main objective of this project was to evaluate the allergenic role of the mite suidasia medanensis. | 2005 | 15575929 |
| quantification of blo t 5 in mite and dust extracts by two-site elisa. | blomia tropicalis is an important mite species in the tropical and sub-tropical regions of the world. blo t 5 is the major allergen with up to 70% sensitization rates in b. tropicalis allergic populations. | 2005 | 15575940 |
| sequence tag catalogs of dust mite-expressed genomes: utility in allergen and acarologic studies. | dust mites are a major source of indoor allergens. they contain a large number of components that react with immunoglobulin (ig) e in individuals with allergies and are capable of inducing sensitization, and allergic respiratory and cutaneous diseases. with a significant proportion of the population affected in some way by mite allergies, it is essential that we improve our understanding of these organisms so that control strategies could be defined and its allergens better understood. thus, we ... | 2004 | 15651897 |
| skin reactivity to aeroallergens is reduced in human t-lymphotropic virus type i-infected healthy blood-donors (asymptomatic carriers). | a type 2 immune response, characterized by high levels of interleukin-4 and immunoglobulin e synthesis is a hallmark of respiratory allergic diseases. individuals infected with human t-lymphotropic virus type i (htlv-i) virus have spontaneous t-cell proliferation and increased interferon gamma production, which are immunological functions associated with a type 1 immune response. | 2005 | 15679726 |
| paradoxical coexistence of atopic asthma and human t-lymphotropic virus type i (htlv-i) infection: a case report. | in this case report, the authors report the presence of two supposedly antagonic immune diseases in the same patient. the patient is a 45-year-old white woman with a history of asthma and allergic rhinitis for the last 10 years. asthmatic symptoms were present and were triggered after exposure to dust and mold. her human t-lymphotropic virus type i (htlv-i) seropositive status was detected by chance five years ago during a routine screening for blood donation. skin prick tests were positive for ... | 2004 | 15736723 |
| bystander effect in synergy to anergy in oral tolerance of blomia tropicalis/ovalbumin murine co-immunization model. | oral tolerance is an important approach in allergic diseases and murine model can provide useful information to improve its understanding and therapeutic measures. to address the influence of non-related allergen sensitization in immunized mice with the mite blomia tropicalis (bt) or ovalbumin (ova) or with both bt/ova allergens. furthermore, we sought to verify oral tolerance effect in the bt/ova co-immunization model. mice sensitized with bt and then exposed to ova developed an enhanced ige re ... | 2005 | 15821892 |
| sensitization to dust mites in children with allergic rhinitis in singapore: does it matter if you scratch while you sneeze? | previously published data established blomia tropicalis, as the major source of allergic sensitization in asthmatic children in tropical singapore. objective to define the prevalence, clinical characteristics and risk factors of species-specific mite sensitization in paediatric allergic rhinitis (ar) patients in this unique environment. | 2005 | 15836750 |
| allergenic cross-reactivity between blomia tropicalis and blomia kulagini (acari: echymiopodidae) extracts from optimized mite cultures. | at present, data about the cross-reactivity of blomia spp. comes from studies made among different genera of mites, and no results have been published involving different species of the genus blomia. | 2005 | 16433207 |
| dust mite species and allergen concentrations in beds of individuals belonging to different urban socioeconomic groups in brazil. | dermatophagoides pteronyssinus, dermatophagoides farinae and blomia tropicalis dust mites are among the most important agents of hypersensitivity reactions in human beings. however, a role of other mites in the etiology of these reactions has not yet been excluded. | 2006 | 16517425 |
| correlation between skin tests to dermatophagoides pteronyssinus, dermatophagoides siboney and blomia tropicalis in cuban asthmatics. | dermatophagoides pteronyssinus, dermatophagoides siboney and blomia tropicalis are the most important allergenic mites in cuba. the aim of this study was to determine the degree of polysensitization and correlation of the skin prick test (spt) reaction size to these mites in asthmatic patients. | 2006 | 16540067 |
| expression of the blomia tropicalis paramyosin blo t 11 and its immunodominant peptide in insect cells. | blo t 11, a dust-mite (blomia tropicalis) paramyosin, is an allergen with significant ige reactivity that has potential as a diagnostic/therapeutic reagent for house-dust-mite allergy. the present study describes the successful expression of blo t 11 and its immunodominant peptide fd in insect cells using a baculovirus expression system. the blo t 11 and fd genes were cloned into the pmelbaca vector and the resulting vectors were co-transfected into sf9 insect cells with bac-n-blue dna. plaque a ... | 2006 | 16584386 |
| molecular characterization, expression and localization of tropomyosin and paramyosin immunodominant allergens from sheep scab mites (psoroptes ovis). | cdnas encoding the immunodominant allergens tropomyosin and paramyosin were amplified from rna extracted from the sheep scab mite psoroptes ovis. the tropomyosin cdna contained an open reading frame (orf) of 852 bp which encoded a predicted protein with 98% and 97% identity to the house dust mite allergens der f 10 and der p 10 respectively. the complete paramyosin orf generated by rt-pcr was 2625 bp in length and encoded an 875aa predicted protein of 102.6 kda with 97%, 95% and 89% identity to ... | 2006 | 16817997 |
| state of the art in developing allergen vaccines in cuba: prospects of novel adjuvanted vaccines. | standardized allergen vaccines have been developed and registered as biopharmaceutical products in cuba. three different vaccines were obtained from the most relevant allergenic mite species: dermatophagoides pteronvssinus, dermatophagoides siboney, and blomia tropicalis. immuno-analytical methods based on murine monoclonal antibodies and human ige antibodies were developed for assessing allergenic potency, composition, and stability. preclinical and clinical studies showed efficacy and safety i ... | 2006 | 16823917 |
| sensitization and exposure to house dust and storage mites in high-altitude areas of ecuador. | few studies have addressed exposure and sensitization to mite allergens in andean countries. | 2006 | 17069110 |
| distribution of allergens in children with different atopic disorders in central taiwan. | allergic disorders, including asthma, allergic rhinitis, and eczema, are the most common chronic childhood diseases. exposure to house dust mites (hdms) can exacerbate allergic disorders in sensitized individuals. the data for sensitization to hdms and other frequent allergens amongst atopic children in taiwan is limited. we studied 498 children (aged 2-16 years) with atopy in central taiwan with cap testing (cationic antimicrobial protein system). our results revealed a high prevalence of sensi ... | 2006 | 17078465 |
| enzymatic activity in extracts of allergy-causing astigmatid mites. | many of the previously characterized allergens of house dust mites are known to be proteases, and this enzymatic activity is thought to contribute to their allergenicity. other astigmatid mites, including stored-product mites and the ectoparasitic itch mite, sarcoptes scabiei de geer, are also known to be allergenic, but little or nothing is known about their enzymatic activities. the purpose of this study was to characterize the enzymatic activities present in extracts of the parasitic itch mit ... | 2006 | 17162954 |
| [indoor allergen sensitization profile in allergic patients of the allergy clinic in the university hospital in uberlândia, brazil]. | to evaluate allergens among patients with allergic respiratory disease attended at the allergy clinic of the university hospital-federal university of uberlândia. | 2007 | 17420889 |
| a nos1 gene polymorphism associated with asthma and specific immunoglobulin e response to mite allergens in a colombian population. | nitric oxide (no) is involved in asthma pathogenesis and is synthesized by three isoforms of no synthase, one of them encoded by nos1 gene. the ca-repeat and the c5266t snp in nos1 exon 29 have been associated with asthma and ige levels. we thought to test the association of asthma and asthma-related phenotypes with the exon 29 ca-repeat and the c5266t snp in a colombian population sample. | 2007 | 17536218 |
| asthma and rhinitis symptoms in individuals from different socioeconomic levels in a brazilian city. | allergy is considered to be caused by complex interactions between genetic and environmental factors. socioeconomic status (ses) may be the most important environmental determinant of allergy because it determines the living environment, but few studies have addressed the causal role of ses in allergy. the aim of this study was to compare the prevalence of asthma and rhinitis symptoms in two ses groups in a brazilian city. history of asthma and rhinitis symptoms was collected using the internati ... | 2007 | 17619568 |
| roles of structure and structural dynamics in the antibody recognition of the allergen proteins: an nmr study on blomia tropicalis major allergen. | blo t 5 is the major allergen from blomia tropicalis mites and shows strong ige reactivity with up to 90% of asthmatic and rhinitis patients' sera. the nmr solution structure of blo t 5 comprises three long alpha helices, forming a coiled-coil, triple-helical bundle with a left-handed twist. trosy-nmr experiments were used to study blo t 5 interaction with the fab' of a specific monoclonal antibody, mab 4a7. the mab epitope comprises two closely spaced surfaces, i and ii, connected by a sharp tu ... | 2008 | 18184590 |
| molecular and biochemical properties of storage mites (except blomia species). | in recent years, the allergological importance of different mite species not belonging to the family pyroglyphidae has been demonstrated. these mites, commonly named storage mites, include lepidoglyphus destructor, glycyphagus domesticus, tyrophagus putrescentiae, acarus siro, aleuroglyphus ovatus, suidasia medanensis and thyreophagus entomophagus. several allergens from these species have been purified, sequenced and cloned. many of these allergens have shown sequence homology and a biological ... | 2007 | 18220992 |
| are cutaneous hypersensitivity tests to inhalant allergens a severity marker for vernal keratoconjunctivitis? | the purpose of this study was to analyze the cutaneous sensitivity to a variety of allergens in patients with vernal keratoconjunctivitis (vkc) and to demonstrate the relation between skin response and clinical aspects of the disease. | 2007 | 18235913 |