| serological relationship between melon yellow spot virus and watermelon silver mottle virus and differential detection of the two viruses in cucurbits. | melon yellow spot virus (mysv), a tentative member of the genus tospovirus, is considered a distinct serotype due to the lack of a serological relationship with other tospoviruses in its nucleocapsid protein (np). recently, a virus isolate collected from diseased watermelon in central taiwan (mysv-tw) was found to react with a rabbit antiserum (ras) prepared against the np of watermelon silver mottle virus (wsmov), and a monoclonal antibody (mab) prepared against the common epitope of the nss pr ... | 2010 | 20480192 |
| development of interspecific solanum lycopersicum and screening for tospovirus resistance. | tospovirus has emerged as a serious viral pathogen for several crops including tomato. the tomato production is being severely affected worldwide by tospovirus. some reports have been published about the association of plant virus and development of human disease either by direct or indirect consumption. resistance to this virus has been identified as good source in wild tomato species (lycopersicum peruvianum). but the introgression of resistance genes into cultivated tomato lines and the devel ... | 2015 | 26587001 |
| pathogen-derived resistance using a viral nucleocapsid gene confers only partial non-durable protection in peanut against peanut bud necrosis virus. | genetic engineering of peanut (arachis hypogaea l.) using the gene encoding for the nucleocapsid protein (n gene) of peanut bud necrosis virus (pbnv; genus tospovirus, family bunyaviridae) was used to impart resistance to bud necrosis disease in peanut (pbnd), a disease for which no durable resistance is available in the existing germplasm. over 200 transgenic lines of peanut var. jl 24 were developed for which integration and expression of the transgenes was confirmed by pcr, southern hybridiza ... | 2013 | 23011312 |
| transgenic resistance by n gene of a peanut bud necrosis virus isolate of characteristic phylogeny. | the nucleocapsid protein (n) gene of a tospovirus devastating tomato crop in the south indian state of tamil nadu was cloned and characterized. the high identity of the cloned sequence to a peanut bud necrosis virus (pbnv) tomato isolate (97.8/99.6% nucleotide/amino acid) and a pbnv peanut isolate (94.4/96.3% nucleotide/amino acid) identified the tospovirus as an isolate of pbnv, designated pbnv coimbatore tomato (pbnv ct) isolate. phylogenetic analysis of pbnv ct n gene provided useful insights ... | 2009 | 19255836 |
| importance and genetic diversity of vegetable-infecting tospoviruses in india. | a survey for peanut bud necrosis virus (pbnv), watermelon bud necrosis virus (wbnv), capsicum chlorosis virus (cacv), and iris yellow spot virus (iysv) was conducted between 2002 and 2009 in the major vegetable-growing areas in india. pbnv was documented widely in tomato and chili peppers in 14 states representing southern, north-western, north-eastern, and central regions and wbnv was predominantly detected in watermelons and cucurbits in all except north-eastern regions. in addition, the expan ... | 2011 | 21299415 |
| comparison of ambisense m rna of watermelon silver mottle virus with other tospoviruses. | abstract double-stranded genomic rnas (dsrnas) extracted from chenopodium quinoa infected with watermelon silver mottle virus (wsmv) were similar to those of tomato spotted wilt virus (tswv, serogroup i) and impatiens necrotic spot virus (insv, serogroup iii), except that the s dsrna of wsmv is 0.75 and 0.6 kbp longer than those of tswv and insv, respectively. the complete nucleotide sequence of the genomic m rna of wsmv was determined from cdna clones generated from separated m dsrna. the m rna ... | 1998 | 18944959 |
| completion of the genome sequence of watermelon silver mottle virus and utilization of degenerate primers for detecting tospoviruses in five serogroups. | abstract the nucleotide sequence of the l rna of watermelon silver mottle virus (wsmov) was determined. combined with the previous work on m and s rnas, the whole genomic sequence of this member of the genus tospovirus was completed. the l rna is 8,917 nucleotides in length, with one large open reading frame encoding a translation product of 2,878 amino acids (331.8 kda) on the viral complementary strand. the l protein shares amino acid identities of only 44.3 and 46.5% with tomato spotted wilt ... | 2001 | 18943848 |
| identification of common epitopes on a conserved region of nss proteins among tospoviruses of watermelon silver mottle virus serogroup. | abstract the nss protein of watermelon silver mottle virus (wsmov) was expressed by a zucchini yellow mosaic virus (zymv) vector in squash. the expressed nss protein with a histidine tag and an additional nia protease cleavage sequence was isolated by ni(2+)-nta resins as a free-form protein and further eluted after sodium dodecyl sulfate-polyacrylamide gel electrophoresis for production of rabbit antiserum and mouse monoclonal antibodies (mabs). the rabbit antiserum strongly reacted with the ns ... | 2006 | 18943661 |
| purification and serological analyses of tospoviral nucleocapsid proteins expressed by zucchini yellow mosaic virus vector in squash. | a plant viral vector engineered from an in vivo infectious clone of zucchini yellow mosaic virus (zymv) was used to express the nucleocapsid proteins (nps) of tospoviruses in planta. the open reading frames (orfs) of nps of different serogroups of tospoviruses, including tomato spotted wilt virus, impatiens necrotic spot virus, watermelon silver mottle virus, peanut bud necrosis virus, and watermelon bud necrosis virus (wbnv), were in frame inserted in between the p1 and hc-pro genes of the zymv ... | 2005 | 15992936 |
| peanut bud necrosis tospovirus s rna: complete nucleotide sequence, genome organization and homology to other tospoviruses. | the complete nucleotide sequence of the s rna of peanut bud necrosis virus (pbnv) has been determined. the rna is 3 057 nucleotides in length, contains inverted repeats and two open reading frames (orfs) with an ambisense coding strategy that are separated by an a+u-rich intergenic region. one orf (1 320 nucleotides in the viral sense strand) encodes a mr 49.5 kda protein, identified as the nonstructural (nss) protein based on similarity to published tospovirus sequences. the second orf (831 nuc ... | 1996 | 8629953 |
| gene effects for parameters of peanut bud necrosis virus (pbnv) resistance in peanut. | the objective of this study was to determine relative importance of gene effects for pbnv incidence and pbnv severity evaluated at 30, 40, 50 and 60 days after planting. eight generations of three crosses involved three parental lines were evaluated for disease incidence (percentage of infected plants) and disease severity under natural occurrence of pbnv infection in a randomized complete block design with six replications. evaluations were carried out at 30, 40, 50 and 60 days after planting ( ... | 2007 | 19069965 |
| genetic organisation of iris yellow spot virus m rna: indications for functional homology between the g(c) glycoproteins of tospoviruses and animal-infecting bunyaviruses. | the complete nucleotide sequence (4838 nucleotides) of iris yellow spot virus (iysv) m rna indicates, typical for tospoviruses, the presence of two genes in ambisense arrangement. the vrna orf codes for the potential cell-to-cell movement (nsm) protein (34.8 kda) and the vcrna orf for the viral glycoprotein (g1/g2) precursor (128.6 kda). multiple sequence alignment of the nsm and g1/g2 precursor proteins of iysv with those of other tospoviruses, showed highest homologies to peanut bud necrosis v ... | 2002 | 12491100 |
| the complete nucleotide sequence and genome organization of the m rna segment of peanut bud necrosis tospovirus and comparison with other tospoviruses. | the m rna of peanut bud necrosis virus (pbnv; synonym groundnut bud necrosis virus) is 4801 nucleotides in length. it comprised two orfs in an ambisense organization and terminal inverted repeats. the 3' large orf (3363 nucleotides in the virus-complementary strand) encoded a protein with a predicted size of 127.2 kda which was identified as the glycoprotein precursor (gp) of the g1 and g2 glycoproteins. a comparison of the deduced amino acid sequence of gp revealed 37% identity and 58-59% simil ... | 1996 | 8811036 |
| multiple artificial micrornas targeting conserved motifs of the replicase gene confer robust transgenic resistance to negative-sense single-stranded rna plant virus. | micrornas (mirnas) regulate the abundance of target mrnas by guiding cleavage at sequence complementary regions. in this study, artificial mirnas (amirnas) targeting conserved motifs of the l (replicase) gene of watermelon silver mottle virus (wsmov) were constructed using arabidopsis pre-mirna159a as the backbone. the constructs included six single amirnas targeting motifs a, b1, b2, c, d of e, and two triple amirnas targeting motifs ab1e or b2dc. processing of pre-amirnas was confirmed by agro ... | 2011 | 21929564 |
| identification and characterization of a novel tospovirus species using a new rt-pcr approach. | a novel tospovirus serologically distinct from all established tospovirus species was found in thailand in physalis minima l. the s rna of this virus was cloned by a new rt-pcr approach revealing a nucleotide sequence of 3257 nucleotides. the ambisense rna segment encoded a nonstructural protein (nss) of 469 amino acids, with a predicted mr of 53.2 kda, and a nucleoprotein (n) of 279 amino acids and a mr of 31.0 kda, so far the largest n protein known for any tospovirus species. n protein sequen ... | 2001 | 11315637 |
| characterization of the large (l) rna of peanut bud necrosis tospovirus. | the nucleocapsids purified from peanut plants systemically infected with peanut bud necrosis virus (pbnv), a member of the genus tospovirus, contained both viral(v) and viral complementary(vc) sense l rnas. defective forms of l rna containing 'core polymerase region' were observed. the full length l rna of pbnv was sequenced using overlapping cdna clones. the 8911 nucleotide l rna contains a single open reading frame (orf) in the vc strand, and encodes a protein of 330 kda. at the 5' and 3' term ... | 1998 | 9930194 |