| analysis of the p1 gene sequences and the 3'-terminal sequences and secondary structures of the single-stranded rna genome of potato virus v. | the immunocapture reverse transcriptase pcr method (ic-rt-pcr) was used to selectively amplify specific genome sequences of an isolate of potato virus v (pvv, genus potyvirus) from a potato plant infected by multiple viruses in the field in finland. the sequences of the 5'- and 3'-non-translated regions (ntr) and the p1-and coat protein (cp)-encoding sequences were determined because they are the most variable genomic regions in potyviruses. the sequences of the new finnish pvv isolate obtained ... | 2001 | 11450952 |
| analysis of putative interactions between potyviral replication proteins and plant retinoblastoma proteins. | sequence comparisons suggest that the rna-dependent rna polymerase (nib) of potyviruses and bymoviruses, as well as the viral polymerase of potexviruses may contain a putative retinoblastoma protein (prb) binding motif. the possibility that the potyviral nib may function in the nucleus through interactions with plant prb-related (rbr) proteins, and the modifications of the cell cycle was investigated by a combination of mutagenesis of the nib and yeast two-hybrid system (yths). mutation of a hig ... | 2002 | 11930964 |
| molecular resolution of a complex of potyviruses infecting solanaceous crops at the centre of origin in peru. | peru is a centre of origin and domestication of the potato, pepper and tomato (family solanaceae). many potyviruses (genus potyvirus) that infect these crops were described 20-30 years ago. however, definitive classification of these viruses as distinct species remains unresolved for several reasons, including their close serological relationships, similar symptomatology in test plants and lack of genomic sequence data. using samples collected from peru, we have determined the complete genomic s ... | 2003 | 12917478 |
| characterization of a new potyvirus infecting pepper crops in ecuador. | sequencing 2,951 nucleotides of the 3' proximal region of the genome of a potyvirus isolate collected from capsicum pubescens (rocoto) pepper in ecuador revealed that this was the first representative of a new species tentatively named ecuadorian rocoto virus (erv). phylogeny reconstruction showed that this isolate clustered with potato virus v (pvv), peru tomato virus and wild potato mosaic virus into a monophyletic group, and was closest to pvv. the isolate was shown to be infectious in tobacc ... | 2008 | 18553171 |
| incidence and characterization of potato virus v infections in iran. | a survey was carried out to determine the extent of potato virus v (pvv) infection, together with other potyviruses, in iran in both commercial and local potato varieties. we found a low incidence of pvv in commercial varieties compared to a local potato cultivar zardi, in which we noted a pvv infection up to ~32.9 %. we determined the genomic sequence 9,812 nucleotides of one isolate (ker.lal.p) from cultivar agria and the 3'-terminal sequence including coat protein (cp) gene of four additional ... | 2014 | 24426313 |
| genome sequence of a divergent colombian isolate of potato virus v (pvv) infecting solanum phureja. | deep sequencing analysis of the transcriptome of a solanum phureja cv. criolla colombia plant with symptoms typical of a virus disease revealed an infection with potato virus v (pvv). the pvv-phureja genome comprises 9904 nt, exhibits 83% nucleotide identity with currently fully sequenced pvv isolates and contains one large orf that codes for a polyprotein of 3065 residues flanked by 5' and 3' utr of 217 and 448 nt, respectively. phylogenetic analysis of the pvv-phureja polyprotein indicates tha ... | 2016 | 26982467 |
| molecular characterization of potato virus v genomes from europe indicates limited spatiotemporal strain differentiation. | abstract because there were no previous reports on the molecular characterization of potato virus v (pvv, genus potyvirus, family potyviridae), the complete genomic sequence of pvv isolate dv42 was determined. the length of the single-stranded messenger-polarity rna genome was 9,851 nt (nucleotides), followed by a poly(a) tail. the genome contained a 5'-terminal nontranslated region (5'-ntr; 204 nt), a single open reading frame (nucleotides 205-9406; 3,067 amino acids), and a 3'-ntr that was unu ... | 2000 | 18944596 |
| genomic sequence of wild potato mosaic virus as compared to the genomes of other potyviruses. | the complete nucleotide sequence of wild potato mosaic virus (wpmv) was determined, showing that it is a distinct member of the genus potyvirus (family potyviridae). the genome consists of 9853 nucleotides and encodes a single polyprotein of 3065 amino acids. the 5'- and 3'-non translated regions (ntr) were 182 and 472 nucleotides, respectively. alignment of the predicted wpmv polyprotein sequence with other members of the genus potyvirus revealed nine putative cleavage sites resulting in ten ma ... | 2003 | 12556999 |
| the complete nucleotide sequence of pepper mottle virus-florida rna. | the pepper mottle virus-florida (pepmov-fl) rna genome was cloned and sequenced, and shown to consist of 9,717 nucleotides (nt) excluding the poly (a) tail. a single open reading frame was identified beginning at nucleotide position 169 encoding a polyprotein of 3068 amino acids. phylogenetic sequence analysis revealed that of 44 full-length viral rna genomes analyzed within the family potyviridae, pepmov-fl was most closely related to pepmov-california (pepmov-ca), potato virus y-h (pvy-h), pvy ... | 2003 | 12536304 |
| a mosaic disease of senna hirsuta induced by a potyvirus in nigeria. | a virus inducing mosaic and severe leaf malformation, isolated from senna hirsuta in nigeria, was studied. the virus had a rather narrow host range, infecting a few species in caesalpinaceae, chenopodiaceae and fabaceae families. the virus was widespread in southern nigeria with prevalence ranging from 74% to 86.4% in some locations. it was transmitted mechanically and in a non-persistent manner by myzuspersicae, aphis craccivora and a. spiraecola. there was no evidence of transmission by seeds. ... | 2001 | 11719985 |
| rapid production of single-chain fv fragments in plants using a potato virus x episomal vector. | we have used a plant virus episomal vector, based on potato virus x (pvx) to transiently express a single-chain fv (scfv) and its diabody derivative in plants. the scfv was directed against a continuous epitope (cryptotope) on the coat protein of potato virus v. a cloned, full-length pvx vector sequence, containing the scfv gene, was used to direct in vitro transcription and the resulting rna was used to inoculate nicotiana clevelandii plants. within a few days, plants developed characteristic s ... | 1999 | 10648933 |